CN211043143U - Correction device for plant chlorophyll fluorescence parameters - Google Patents
Correction device for plant chlorophyll fluorescence parameters Download PDFInfo
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- CN211043143U CN211043143U CN201921656657.8U CN201921656657U CN211043143U CN 211043143 U CN211043143 U CN 211043143U CN 201921656657 U CN201921656657 U CN 201921656657U CN 211043143 U CN211043143 U CN 211043143U
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Abstract
The utility model provides a correction device for plant chlorophyll fluorescence parameters, which comprises a control module and a box body, wherein the side wall of the box body is provided with a reflector, and the box body can contain a plant to be measured; a light source component and a camera are arranged on the top plate of the box body; the photographing direction of the camera points to the plants in the box; a lens is arranged in front of the camera, and a filter element is arranged between the camera and the lens; the light source assembly can change the illumination environment of the plant under the control of the control module; the light that light filtering element can enter the light end to the camera under control module control filters, the utility model discloses can accurately detect photosynthesis efficiency more.
Description
Technical Field
The utility model belongs to the technical field of plant phenotype analysis technique and specifically relates to a correcting unit of plant chlorophyll fluorescence parameter.
Background
Photosynthetic efficiency is an important trait in plant phenotype and is one of the key points of research. The chloroplast is used as a mechanism of photosynthesis, and the rapid nondestructive monitoring of the physiological state of the chloroplast is an important link for realizing high-flux photosynthesis analysis. Chlorophyll fluorescence is a "probe" of plant photosynthesis, and can reflect the real-time plant photosynthesis efficiency. However, when measuring chlorophyll fluorescence signals of plant leaves, the behavior (aggregation and movement) that chloroplasts can move under illumination is often ignored, so that the measured fluorescence yield is deviated, the accuracy of measuring the photosynthesis efficiency is reduced, and the selection of varieties with excellent photosynthesis efficiency is not facilitated.
Disclosure of Invention
The utility model provides a correcting unit of plant chlorophyll fluorescence parameter can be used to the correction of original chlorophyll fluorescence image in order to obtain real chlorophyll fluorescence image, can realize the purpose of more accurate detection photosynthesis efficiency.
The utility model adopts the following technical scheme.
A correction device for plant chlorophyll fluorescence parameters comprises a control module and a box body, wherein a reflector is arranged on the side wall of the box body, and the box body can accommodate a plant to be measured; a light source component and a camera are arranged on the top plate of the box body; the photographing direction of the camera points to the plants in the box; a lens is arranged in front of the camera, and a filter element is arranged between the camera and the lens; the light source assembly can change the illumination environment of the plant under the control of the control module; the light filtering element can filter the light rays emitted to the light inlet end of the camera under the control of the control module.
A conveying belt is arranged in the box body; the transmission belt is provided with a hole tray for loading plants; the transmission band can be sent into or send the box with the plant.
The camera is a monochrome CCD camera.
The filtering element is a filtering wheel; six working positions are arranged on the filtering wheel; the working positions comprise five filtering positions and a zero position; the zero position does not filter light; each light filtering position is provided with a light filter for filtering light; the filters of each filtering position are respectively a 680 nm band-pass filter, a 440 nm band-pass filter, a 520nm band-pass filter, a 690 nm band-pass filter and a 740 nm band-pass filter.
the light source assembly comprises a plurality of annular L ED light sources, the camera is arranged in the center of the ring where the L ED light sources are arranged, and the plants are located below the camera during measurement.
the L ED light source comprises an actinic light source with the central wavelength of 620nm and an ultraviolet light source with the central wavelength of 400nm, wherein the actinic light source is used for exciting plant dynamic chlorophyll fluorescence and providing a light source for obtaining a plant reflection image, and the ultraviolet light source is used for exciting plant steady chlorophyll fluorescence.
The device adopts a correction method of the plant chlorophyll fluorescence parameter for measuring the plant chlorophyll fluorescence parameter, and the method comprises the following steps;
A1, placing the plant in a dark environment, and carrying out dark adaptation treatment on the plant to reset the photosynthetic system of the plant to an initial state;
A2, in a dark environment, measuring light illumination, acquiring a minimum chlorophyll fluorescence image of the plant after dark adaptation treatment by a camera through a filter element, and acquiring a maximum chlorophyll fluorescence image of the plant after dark adaptation treatment by the camera through the filter element by oversaturated light illumination;
A3, exposing the plant to the environment of actinic light, and immediately acquiring an initial reflection image of the plant exposed to the environment of actinic light through a camera;
A4, irradiating the plants with actinic light for t time, and acquiring reflected images of the plants irradiated with the actinic light for t time by a camera after the plants reach a light adaptation state; then a camera is used for obtaining the plant dynamic instantaneous fluorescence image at the moment through a light filtering element;
A5, after the plant is in an ultraviolet light illumination environment for a time t, a camera is used for obtaining a blue spectrum fluorescence image, a green spectrum fluorescence image, a red spectrum fluorescence image and a far infrared spectrum fluorescence image of the plant steady state after the plant is irradiated by the ultraviolet light for the time t through a light filtering element;
A6, obtaining the chlorophyll yield Ro at the moment according to the initial reflection image of the plant in the actinic light irradiation environment; obtaining the chlorophyll yield Rt at the time according to the reflection image of the plant irradiated by the long-time actinic light at t, and calculating the deviation coefficient of the chlorophyll fluorescence yield caused by chloroplast movement under the illumination condition according to the formula c =1+ ((Rt-Ro)) ⁄ Ro;
A7, correcting the dynamic and steady chlorophyll fluorescence yield to obtain actual chlorophyll fluorescence yield parameters under illumination conditions;
And A8, performing mathematical operation on the corrected chlorophyll fluorescence yield parameters to obtain a chlorophyll fluorescence parameter image capable of reflecting the actual photosynthesis of the plant for analysis.
In the step A1, the dark adaptation treatment time for the plant is not less than 25 minutes, and the intensity of the dark adaptation treatment can be about 10. mu. mol. m -2·s-1The 740nm far infrared light of (1) is irradiated to the plant.
In step a3, the actinic light irradiation environment simulates a natural environment with actinic light of varying light intensity; the pattern of light intensity variations comprises one or more of a constant light intensity, a sin variation, a cos variation or a step variation.
The chlorophyll fluorescence parameters comprise non-actinic light quenching coefficients, minimum fluorescence variable fluorescence in a light adaptation state and steady-state fluorescence parameters in the light adaptation state.
Compared with the prior art, the beneficial effects of the utility model are embodied in:
(1) The utility model discloses a method and device can overcome the removal that the chloroplast arouses the response of setting a camera to the light and cause chlorophyll fluorescence signal measuring deviation, can be more accurate acquire the photosynthesis information of plant
(2) The utility model discloses a method and device pass through the different illumination mode of program setting, simulate natural condition's light intensity change, strengthen the difference of plant to different irradiant photoresponse, realize the accurate detection of plant photosynthesis efficiency.
(3) The obtained corrected fluorescence signals comprise dynamic fluorescence and steady-state fluorescence, and the photosynthesis difference of plants with different genotypes can be explained from different photosynthesis scales.
The utility model provides a chlorophyll fluorescence parameter's correction method and device is through the fluorescence image under the dark adaptation that obtains the plant respectively and the illumination condition to gather the red light reflection image that illumination initial moment and t correspond constantly, calculate the deviation coefficient, be used for the correction of original chlorophyll fluorescence image, finally obtain real chlorophyll fluorescence image, realize the purpose that photosynthesis efficiency is accurate more detected.
Drawings
The invention will be described in further detail with reference to the following drawings and detailed description:
Figure 1 is a schematic view of the apparatus of the present invention;
FIG. 2 is a schematic view of the top plate of the box body of the present invention;
FIG. 3 is a schematic flow diagram of the method of the present invention;
In the figure: 1-a camera; 2-a plant; 3-plug tray; 4, conveying the belt; 5-a box body; 6-source of actinic light; 7-ultraviolet light source; 14-a filter wheel; 15-a control module; 201-lens; 202-working bit.
Detailed Description
1-3, a device for correcting fluorescence parameters of plant chlorophyll, said device being used in the above method;
The correcting device comprises a control module 15 and a box body, wherein a reflector is arranged on the side wall of the box body, and the box body can contain the plant 2 to be measured; a light source component and a camera 1 are arranged on the top plate of the box body; the photographing direction of the camera points to the plants in the box; a lens 201 is arranged in front of the camera, and a filter element is arranged between the camera and the lens; the light source assembly can change the illumination environment of the plant under the control of the control module; the light filtering element can filter the light rays emitted to the light inlet end of the camera under the control of the control module.
A conveying belt 4 is arranged in the box body; the transmission belt is provided with a hole tray 3 for loading plants; the transmission band can be sent into or send the box with the plant.
The camera is a monochrome CCD camera.
The filter element is a filter wheel 14; six working positions 202 are arranged on the filter wheel; the working positions comprise five filtering positions and a zero position; the zero position does not filter light; each light filtering position is provided with a light filter for filtering light; the filters of each filtering position are respectively a 680 nm band-pass filter, a 440 nm band-pass filter, a 520nm band-pass filter, a 690 nm band-pass filter and a 740 nm band-pass filter.
the light source assembly comprises a plurality of annular L ED light sources, the camera is arranged in the center of the ring where the L ED light sources are arranged, and the plants are located below the camera during measurement.
the L ED light source comprises an actinic light source 6 with the central wavelength of 620nm and an ultraviolet light source 7 with the central wavelength of 400nm, wherein the actinic light source is used for exciting plant dynamic chlorophyll fluorescence and providing a light source for obtaining a plant reflection image, and the ultraviolet light source is used for exciting plant steady-state chlorophyll fluorescence.
The device uses a correction method of the plant chlorophyll fluorescence parameter for measuring the plant chlorophyll fluorescence parameter, and the method comprises the following steps;
A1, placing the plant in a dark environment, and carrying out dark adaptation treatment on the plant to reset the photosynthetic system of the plant to an initial state;
A2, in a dark environment, measuring light illumination, acquiring a minimum chlorophyll fluorescence image of the plant after dark adaptation treatment by a camera through a filter element, and acquiring a maximum chlorophyll fluorescence image of the plant after dark adaptation treatment by the camera through the filter element by oversaturated light illumination;
A3, exposing the plant to the environment of actinic light, and immediately acquiring an initial reflection image of the plant exposed to the environment of actinic light through a camera;
A4, irradiating the plants with actinic light for t time, and acquiring reflected images of the plants irradiated with the actinic light for t time by a camera after the plants reach a light adaptation state; then a camera is used for obtaining the plant dynamic instantaneous fluorescence image at the moment through a light filtering element;
A5, after the plant is in an ultraviolet light illumination environment for a time t, a camera is used for obtaining a blue spectrum fluorescence image, a green spectrum fluorescence image, a red spectrum fluorescence image and a far infrared spectrum fluorescence image of the plant steady state after the plant is irradiated by the ultraviolet light for the time t through a light filtering element;
A6, obtaining the chlorophyll yield Ro at the moment according to the initial reflection image of the plant in the actinic light irradiation environment; obtaining the chlorophyll yield Rt at the time according to the reflection image of the plant irradiated by the long-time actinic light at t, and calculating the deviation coefficient of the chlorophyll fluorescence yield caused by chloroplast movement under the illumination condition according to the formula c =1+ ((Rt-Ro)) ⁄ Ro;
A7, correcting the dynamic and steady chlorophyll fluorescence yield to obtain actual chlorophyll fluorescence yield parameters under illumination conditions;
And A8, performing mathematical operation on the corrected chlorophyll fluorescence yield parameters to obtain a chlorophyll fluorescence parameter image capable of reflecting the actual photosynthesis of the plant for analysis.
In the step A1, the dark adaptation treatment time for the plant is not less than 25 minutes, and the intensity of the dark adaptation treatment can be about 10. mu. mol. m -2·s-1The 740nm far infrared light of (1) is irradiated to the plant.
In step a3, the actinic light irradiation environment simulates a natural environment with actinic light of varying light intensity; the pattern of light intensity variations comprises one or more of a constant light intensity, a sin variation, a cos variation or a step variation.
The chlorophyll fluorescence parameters comprise non-actinic light quenching coefficients, minimum fluorescence variable fluorescence in a light adaptation state and steady-state fluorescence parameters in the light adaptation state.
Example (b):
As shown in fig. 1, the apparatus of the correction method for chlorophyll fluorescence parameters includes an imaging system, which is composed of a CCD monochrome camera 1, a 10 mm lens, and a filter wheel 14. Six working positions on the filter wheel included a 680nm bandpass filter, narrow band 440 nm, 520 nm, 690 nm and 740 nm filters, and a null (no filter). A 680nm band-pass filter used for dynamic fluorescence measurement, filters with the narrow bands of 440 nm, 520 nm, 690 nm and 740 nm used for multispectral fluorescence measurement, and a zero position (without a filter) used for red light reflectivity image acquisition. Plants 2 of different genotypes are planted in the hole trays 3 in the same way, and the hole trays are placed on the conveying belt 4, so that the plants in different hole trays can enter the visual field range of the camera. In order to obtain images of dynamic fluorescence, steady-state fluorescence and red light reflectivity, the system provides 4 actinic light sources (620 nm) and 4 ultraviolet lights (400 nm), and the light sources are respectively arranged around the camera and are uniformly distributed above the bottom of the box body, so that uniform light sources are provided for plants.
The flow chart of the correction process of chlorophyll fluorescence parameters is shown in figure 3,
(1) Under weak far-red light, the plants were first dark-adapted in a dark room of the box 5 for 25 minutes.
(2) The control module controls the filter wheel to turn to the position of the 680 nm band-pass filter, and the measuring light (the intensity is about 1 mu mol.m) -2·s-1) The camera acquires the minimal fluorescence image and after 3 seconds turns on the saturation light (2000. mu. mol. m) -2·s-1) The camera acquires a maximum fluorescence image. Program control Turning the filter wheel to a zero position, turning on actinic light, and immediately acquiring a reflection image of the plant by the camera;
(3) Continuously irradiating the plant with actinic light, and acquiring a reflection image of the plant at the t moment by a camera at the t moment; then controlling the filter wheel to turn to the position of the 680 nm band-pass filter by a program, acquiring a dynamic instantaneous fluorescence image at the moment, turning on saturated light, and acquiring the maximum fluorescence image under the illumination condition again by the camera;
(4) Turning off actinic light, turning on an ultraviolet light source, controlling the filter wheel to rotate to the position of a 440 nm narrow-band filter by a program to obtain a blue spectral fluorescence image, then controlling the filter wheel to rotate to the position of a 520nm narrow-band filter by the program to obtain a green spectral fluorescence image, then controlling the filter wheel to rotate to the position of a 690nm narrow-band filter by the program to obtain a red spectral fluorescence image, and controlling the filter wheel to rotate to the position of a 740nm narrow-band filter by a cut-off program to obtain a far red spectral fluorescence image;
(5) Calculating a deviation coefficient c =1+ ((Rt-Ro)) ⁄ Ro of chlorophyll fluorescence yield due to chloroplast movement under light conditions;
(6) Correcting the dynamic and steady chlorophyll fluorescence yield to obtain the actual chlorophyll fluorescence yield under the illumination condition;
(7) Performing mathematical operation on the corrected chlorophyll fluorescence parameters to obtain a chlorophyll fluorescence parameter image capable of reflecting the actual photosynthesis of the plant for analysis, wherein the dynamic fluorescence parameters comprise non-actinic light quenching coefficients and minimum fluorescence variable fluorescence under light adaptation;
(8) And controlling the next plant to be detected in the aperture disk to enter the camera view field by a program, circulating the steps, detecting all the plants, analyzing the photosynthesis efficiency of the plants with different genotypes, and screening the varieties with excellent photosynthesis efficiency.
In this example, the half-bandwidths of the four narrowband filters, namely the narrowband 440 nm, the narrowband 520 nm, the narrowband 690 nm and the narrowband 740 nm, are all 15 nm, and the control program and the analysis program which are built in the control module are written in C + +.
The above are only specific embodiments of the present invention, and all the changes and modifications made according to the claims of the present invention shall belong to the coverage of the present invention.
Claims (6)
1. A correction device of plant chlorophyll fluorescence parameter is characterized in that: the correcting device comprises a control module and a box body, wherein a reflector is arranged on the side wall of the box body, and the box body can contain plants to be measured; a light source component and a camera are arranged on the top plate of the box body; the photographing direction of the camera points to the plants in the box; a lens is arranged in front of the camera, and a filter element is arranged between the camera and the lens; the light source assembly can change the illumination environment of the plant under the control of the control module; the light filtering element can filter the light rays emitted to the light inlet end of the camera under the control of the control module.
2. The device for correcting the fluorescence parameters of plant chlorophyll according to claim 1, wherein: a conveying belt is arranged in the box body; the transmission belt is provided with a hole tray for loading plants; the transmission band can be sent into or send the box with the plant.
3. The device for correcting the fluorescence parameters of plant chlorophyll according to claim 1, wherein: the camera is a monochrome CCD camera.
4. The device for correcting the fluorescence parameters of plant chlorophyll according to claim 3, wherein: the filtering element is a filtering wheel; six working positions are arranged on the filtering wheel; the working positions comprise five filtering positions and a zero position; the zero position does not filter light; each light filtering position is provided with a light filter for filtering light; the filters of each filtering position are respectively a 680 nm band-pass filter, a 440 nm band-pass filter, a 520nm band-pass filter, a 690 nm band-pass filter and a 740 nm band-pass filter.
5. the device for correcting the fluorescence parameters of plant chlorophyll according to claim 3, wherein said light source assembly comprises a plurality of LED light sources arranged in a ring shape, said camera is disposed at the center of the ring shape of the L ED light sources, and said plant is located under the camera during measurement.
6. the device for correcting the fluorescence parameters of plant chlorophyll according to claim 5, wherein the L ED light source comprises an actinic light source with a central wavelength of 620nm and an ultraviolet light source with a central wavelength of 400nm, the actinic light source is used for exciting plant dynamic chlorophyll fluorescence and providing a light source for obtaining a plant reflection image, and the ultraviolet light source is used for exciting plant steady chlorophyll fluorescence.
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| CN201921656657.8U CN211043143U (en) | 2019-09-30 | 2019-09-30 | Correction device for plant chlorophyll fluorescence parameters |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110554017A (en) * | 2019-09-30 | 2019-12-10 | 福建农林大学 | Method and device for correcting fluorescence parameters of plant chlorophyll |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110554017A (en) * | 2019-09-30 | 2019-12-10 | 福建农林大学 | Method and device for correcting fluorescence parameters of plant chlorophyll |
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Granted publication date: 20200717 Termination date: 20210930 |