CN210796436U

CN210796436U - Reaction tube heating device for PCR amplification

Info

Publication number: CN210796436U
Application number: CN201921643565.6U
Authority: CN
Inventors: 贾晶; 许小将; 蒋岩; 任雅南
Original assignee: Shanxi Shining High Tech Medical Testing Center Co Ltd
Current assignee: Shanxi Shining High Tech Medical Testing Center Co Ltd
Priority date: 2019-09-29
Filing date: 2019-09-29
Publication date: 2020-06-19
Anticipated expiration: 2029-09-29

Abstract

The utility model discloses a PCR amplifys uses reaction tube heating device, including the heating cabinet, the heating cabinet is hollow cuboid structure, the top swing joint of heating cabinet has the second closing plate, the bottom fixedly connected with axle sleeve of second closing plate, the side of heating cabinet is provided with the drain pipe, the side-mounting of heating cabinet has the thermometer. The utility model discloses a set up the supporter, the nut, the slider, the threaded rod, a spring, splint convenience carry out the centre gripping to unidimensional reaction tube, the centre gripping is effectual, the flexibility that improves the device and use, through setting up feed liquor pipe, the drain pipe, the heating wire, conveniently heat the liquid in the device, heat to the reaction tube by liquid, the temperature in the convenient to use controlling means of cooperation, simultaneously through setting up motor, the apparatus further comprises a rotating shaft, the carousel, the supporter is convenient carries out the even heating to the reaction tube, it is effectual to heat, improve the accuracy of experimental result simultaneously.

Description

Reaction tube heating device for PCR amplification

Technical Field

The utility model relates to a PCR amplifys technical field, especially relates to a reaction tube heating device for PCR amplifys.

Background

PCR is a short for polymerase chain reaction, and is a method for synthesizing specific DNA fragment by in vitro enzymatic synthesis, wherein a period is formed by high-temperature denaturation, low-temperature annealing (renaturation), moderate-temperature extension and other reactions, and the reaction is performed in a circulating manner, so that the target DNA can be rapidly amplified, and the method has the characteristics of strong specificity, high sensitivity, simple and convenient operation, time saving and the like.

Because PCR is the polymerase chain reaction in-process, need carry out temperature control to each stage, among the current device, put thing groove structure fixed, directly put into the standing groove with the reaction tube, but the diameter of different reaction tubes is mostly different, consequently, when placing different reaction tubes simultaneously, the fixed effect of reaction tube is not good to the reaction tube temperature that is close to the heat source when heating is higher than the reaction tube of keeping away from the heat source, and heating effect is poor, influences the accuracy of experimental result.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects in the prior art and providing a heating device of a reaction tube for PCR amplification.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a reaction tube heating device for PCR amplification comprises a heating box, wherein the heating box is of a hollow cuboid structure, the top of the heating box is movably connected with a second sealing plate, the bottom of the second sealing plate is fixedly connected with an axle sleeve, a liquid outlet pipe is arranged on the side surface of the heating box, a thermometer is arranged on the side surface of the heating box, a first sealing plate is fixed inside the heating box, a mounting seat is fixed on the top of the first sealing plate, the mounting seat is of a circular ring structure, a groove is formed in the top of the mounting seat, frames are arranged at two ends of the bottom of the first sealing plate, an electric heating wire is arranged inside the frames, a heat insulation box is arranged at the bottom of the first sealing plate, a motor is arranged inside the heat insulation box, an output shaft of the motor penetrates through the first sealing plate and is connected with a rotating shaft, and the top of the rotating shaft is, the rotating shaft is connected with a rotating disc through a spline, the bottom of the rotating disc is provided with a slide rail, the slide rail is connected with the mounting seat through a groove in a sliding way, a rack is fixedly sleeved on the rotating shaft, a storage groove is arranged on the rack, threaded rods are arranged on two sides of the storage groove, the screw cap is sleeved on one end of the threaded rod far away from the article placing rack through threads, the threaded rod is inserted into the article placing groove and is connected with the sliding block, the sliding block is connected with the object placing groove in a sliding way, a spring is arranged between the sliding block and the side surface of the object placing groove, two ends of the side surface of the sliding block are fixedly connected with connecting rods, a clamping plate is connected between one ends of the two connecting rods far away from the sliding block, a reaction tube is movably connected between the two clamping plates, the top of reaction tube has cup jointed the seal cover, the feed liquor pipe has been run through to one side that the drain pipe was kept away from to the heating cabinet, the top of heating cabinet is articulated through the hinge has the case lid.

Preferably, the liquid outlet pipe and the liquid inlet pipe are both provided with valves.

Preferably, the bottom of heating cabinet has the landing leg welded, four the landing leg is located the four corners of the bottom of heating cabinet.

Preferably, the material of the heat insulation box is glass fiber, and the material of the first sealing plate is diamond.

Preferably, the mounting seat, the sliding rail, the rotating shaft and the rotating disc are coaxial.

Preferably, the clamping plate is of a circular arc structure.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model has the advantages that reaction tubes with different sizes can be conveniently clamped by arranging the storage rack, the screw cap, the slide block, the threaded rod, the spring and the clamping plate, the clamping effect is good, and the use flexibility of the device is improved;

2. the utility model discloses in through setting up feed liquor pipe, drain pipe, heating wire, conveniently heating the liquid in the device, heat to the reaction tube by liquid, the temperature in the convenient to use controlling means of cooperation is simultaneously through setting up motor, pivot, carousel, supporter convenience and evenly heating the reaction tube, and heating effect is good, improves the accuracy of experimental result simultaneously.

Drawings

FIG. 1 is a schematic structural view of a heating device for a PCR amplification reaction tube according to the present invention;

FIG. 2 is a plan view of the rack of the heating device for PCR amplification reaction tube according to the present invention.

In the figure: 1 heating box, 2 drain pipes, 3 first sealing plates, 4 frames, 5 heating wires, 6 heat insulation boxes, 7 motors, 8 rotating shafts, 9 mounting seats, 10 sliding rails, 11 rotating discs, 12 storage racks, 13 clamping plates, 14 screw caps, 15 threaded rods, 16 springs, 17 sliding blocks, 18 connecting rods, 19 liquid inlet pipes, 20 box covers, 21 second sealing plates, 22 shaft sleeves, 23 sealing sleeves, 24 reaction pipes and 25 thermometers.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-2, a reaction tube heating device for PCR amplification comprises a heating box 1, the heating box 1 is a hollow cuboid structure, a second sealing plate 21 is movably connected to the top of the heating box 1, a shaft sleeve 22 is fixedly connected to the bottom of the second sealing plate 21, a liquid outlet tube 2 is arranged on the side of the heating box 1, a thermometer 25 is arranged on the side of the heating box 1, a first sealing plate 3 is fixed inside the heating box 1, a mounting seat 9 is fixed on the top of the first sealing plate 3, the mounting seat 9 is of a circular structure, a groove is formed in the top of the mounting seat 9, frames 4 are arranged at two ends of the bottom of the first sealing plate 3, an electric heating wire 5 is arranged inside the frames 4, a heat insulation box 6 is arranged at the bottom of the first sealing plate 3, a motor 7 is arranged inside the heat insulation box 6, an output shaft of the motor 7 penetrates through the first sealing plate 3 and is connected with a rotating, there is carousel 11 through splined connection in pivot 8, slide rail 10 is installed to carousel 11's bottom, slide rail 10 passes through recess and 9 sliding connection of mount pad, fixed cover has been connected with supporter 12 in the pivot 8, it puts the thing groove to have seted up on supporter 12, put the both sides in thing groove and install threaded rod 15, threaded rod 15 keeps away from the one end of supporter 12 and has cup jointed nut 14 through the screw thread, threaded rod 15 inserts to put the thing inslot and is connected with slider 17, slider 17 and put thing groove sliding connection, install spring 16 between the side of slider 17 and thing groove, the both ends fixedly connected with connecting rod 18 of slider 17 side, be connected with splint 13 between the one end that slider 17 was kept away from to two connecting rods 18, swing joint has reaction tube 24 between two splint 13, seal cover 23 has been cup jointed at reaction tube 24's top, one side that drain pipe 2 was kept away from to heating cabinet 1 runs through feed.

Wherein, the liquid outlet pipe 2 and the liquid inlet pipe 19 are both provided with valves.

Wherein, the welding of the bottom of heating cabinet 1 has the landing leg, and four landing legs are located the four corners of the bottom of heating cabinet 1.

The heat insulation box 6 is made of glass fiber, and the first sealing plate 3 is made of diamond.

Wherein, the mounting seat 9, the slide rail 10, the rotating shaft 8 and the turntable 11 are coaxial.

Wherein, the clamping plate 13 is in a circular arc structure.

The working principle is as follows: the reaction tube is placed between the two clamping plates 13, the screw cap 14 is rotated, the screw cap 14 drives the threaded rod 15, the threaded rod 15 drives the sliding block 17, the sliding block 17 drives the connecting rod 18, the connecting rod 18 drives the clamping plates 13, the distance between the two clamping plates 13 is adjusted by rotating the screw cap 14, and the reaction tube is clamped through the spring 16, so that the reaction tube clamping device is suitable for clamping reaction tubes with different sizes, the clamping effect is good, and the device is flexible to use;

when the device is used, the box cover 20 is closed, liquid is added into the device through the liquid inlet pipe 19, the liquid adding is stopped and the valve is closed when the liquid level is flush with the lower surface of the shelf 12, the device is electrified, the heating wire 5 and the motor 7 are opened, the heating wire 5 is electrified to generate heat, the liquid in the device is heated through the first sealing plate 3, the temperature of the device is observed through the thermometer 25, the motor 7 drives the rotating shaft 8, the rotating shaft 8 drives the rotating disc 11, the rotating disc 11 drives the shelf 12, the shelf 12 drives the reaction pipe to enable the reaction pipe to move at a constant speed and to enable the reaction pipe to be uniformly heated, when the temperature reaches ninety ℃, the heating wire 5 is closed to stop heating, at the moment, the first stage (high temperature denaturation) of PCR in the reaction pipe is ended, the valve is opened, the liquid at room temperature is slowly injected through the liquid inlet pipe 19, the liquid in the device is slowly discharged through the liquid, the valve is closed to maintain liquid in the device at fifty ℃ for a period of time, the second PCR stage (low-temperature annealing) in the reaction tube is finished at the moment, the heating wire 5 is opened, the device reaches seventy ℃ by observing the thermometer 25 and the heating wire 5 is closed, the liquid in the device is maintained at seventy ℃ for a period of time, the third PCR stage (proper temperature extension) in the reaction tube is finished at the moment, the PCR is finished at the moment, the motor is closed, the reaction tube is convenient to be uniformly heated and the temperature is controlled, the reaction accuracy is improved, meanwhile, water is preferably used as a heating medium, the cost is saved, and the pollution is reduced.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The heating device for the reaction tube for PCR amplification comprises a heating box (1) and is characterized in that the heating box (1) is of a hollow cuboid structure, a second sealing plate (21) is movably connected to the top of the heating box (1), a shaft sleeve (22) is fixedly connected to the bottom of the second sealing plate (21), a liquid outlet tube (2) is arranged on the side face of the heating box (1), a thermometer (25) is installed on the side face of the heating box (1), a first sealing plate (3) is fixed inside the heating box (1), a mounting seat (9) is fixed to the top of the first sealing plate (3), the mounting seat (9) is of a ring-shaped structure, a groove is formed in the top of the mounting seat (9), frames (4) are installed at two ends of the bottom of the first sealing plate (3), and heating wires (5) are arranged inside the frames (4), the heat insulation box (6) is installed at the bottom of the first sealing plate (3), a motor (7) is installed inside the heat insulation box (6), an output shaft of the motor (7) penetrates through the first sealing plate (3) and is connected with a rotating shaft (8), the top of the rotating shaft (8) is rotatably connected with a shaft sleeve (22), a rotating plate (11) is connected onto the rotating shaft (8) through a spline, a sliding rail (10) is installed at the bottom of the rotating plate (11), the sliding rail (10) is slidably connected with a mounting seat (9) through a groove, a storage rack (12) is fixedly sleeved on the rotating shaft (8), a storage groove is formed in the storage rack (12), threaded rods (15) are installed on two sides of the storage groove, a nut (14) is sleeved at one end, far away from the storage rack (12), of each threaded rod (15), is inserted into the storage groove and connected with a sliding block (, slider (17) with put thing groove sliding connection, slider (17) and put and install spring (16) between the side in thing groove, both ends fixedly connected with connecting rod (18) of slider (17) side, two be connected with splint (13) between the one end of slider (17) is kept away from in connecting rod (18), two swing joint has reaction tube (24) between splint (13), seal cover (23) have been cup jointed at the top of reaction tube (24), feed liquor pipe (19) have been run through to one side that drain pipe (2) were kept away from in heating cabinet (1), the top of heating cabinet (1) is passed through the hinge and is articulated to have case lid (20).

2. The heating apparatus for a reaction tube used in PCR amplification of claim 1, wherein the liquid outlet tube (2) and the liquid inlet tube (19) are provided with valves.

3. The heating apparatus for a reaction tube for PCR amplification according to claim 1, wherein the heating chamber (1) has legs welded to the bottom thereof, and four legs are provided at four corners of the bottom of the heating chamber (1).

4. The heating device for a reaction tube for PCR amplification according to claim 1, wherein the heat insulating box (6) is made of glass fiber, and the first sealing plate (3) is made of diamond.

5. The heating device for a reaction tube for PCR amplification according to claim 1, wherein the mounting seat (9), the slide rail (10), the rotating shaft (8), and the turntable (11) are coaxial.

6. The heating device for a reaction tube for PCR amplification according to claim 1, wherein the holder plate (13) has a circular arc-shaped configuration.