CN220450203U - Reaction tube heating device for PCR amplification - Google Patents
Reaction tube heating device for PCR amplification Download PDFInfo
- Publication number
- CN220450203U CN220450203U CN202321532377.2U CN202321532377U CN220450203U CN 220450203 U CN220450203 U CN 220450203U CN 202321532377 U CN202321532377 U CN 202321532377U CN 220450203 U CN220450203 U CN 220450203U
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- pcr amplification
- reaction tube
- heating
- plate
- handle
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 64
- 238000010438 heat treatment Methods 0.000 title claims abstract description 53
- 238000012408 PCR amplification Methods 0.000 title claims abstract description 52
- 238000001816 cooling Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005485 electric heating Methods 0.000 abstract 1
- 238000000137 annealing Methods 0.000 description 14
- 238000004925 denaturation Methods 0.000 description 11
- 230000036425 denaturation Effects 0.000 description 11
- 230000006978 adaptation Effects 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000004153 renaturation Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
Abstract
The utility model relates to the technical field of PCR amplification and discloses a reaction tube heating device for PCR amplification, which comprises a heating box, wherein an electric telescopic rod is arranged in the heating box, the output end of the electric telescopic rod is fixedly connected with a supporting seat, the top of the supporting seat is rotationally connected with a lifting plate, the top wall of the lifting plate is provided with three clamping devices, the part of the lifting plate corresponding to the clamping devices is provided with three vertical holes, the heating box is internally provided with three placing cavities, the placing cavities correspond to the vertical holes, the bottom of the placing cavities is fixedly provided with a heating plate, the inside of the heating plate is provided with an electric heating wire, the inside of the placing cavities is provided with a liner, and the clamping devices comprise connecting blocks; the utility model has the effects of saving time and resources without continuously heating and cooling to change the temperature, and simultaneously can clamp the reaction tubes with different diameters.
Description
Technical Field
The utility model relates to the technical field of PCR amplification, in particular to a reaction tube heating device for PCR amplification.
Background
PCR is a short term of polymerase chain reaction, is a method for synthesizing specific DNA fragments by in vitro enzymatic synthesis, and consists of a cycle of reactions such as high-temperature denaturation, low-temperature annealing (renaturation), temperature-adaptive extension and the like, and is circularly carried out, so that target DNA can be rapidly amplified.
Because PCR is a polymerase chain reaction, in the process, the reaction tube is required to be denatured at a high temperature of 90-95 ℃, then renatured at 55-60 ℃, finally the reaction tube is required to be stretched at a proper temperature of 70-75 ℃ and repeated for many times, in the existing device, the reaction is continuously carried out by changing the temperature through heating or cooling, but the continuous heating and cooling wastes much time and energy, so that the efficiency of the PCR amplification reaction is low, meanwhile, the diameters of different reaction tubes are mostly different, and the reaction tubes are not well fixed when the different reaction tubes are placed.
Accordingly, there is a need to provide a reaction tube heating device for PCR amplification that solves the above-mentioned problems.
Disclosure of Invention
The utility model aims to provide a reaction tube heating device for PCR amplification, which has the effects of saving time and resources and clamping reaction tubes with different diameters without continuously heating and cooling for changing temperature.
The technical aim of the utility model is realized by the following technical scheme: the utility model provides a PCR amplification is with reaction tube heating device, includes the heating cabinet, the inside of heating cabinet is provided with electric telescopic handle, electric telescopic handle's output fixedly connected with supporting seat, the top of supporting seat rotates and is connected with the lifter plate, three clamping device is installed to the roof of lifter plate, three perpendicular hole has been seted up with clamping device's corresponding position on the lifter plate, three place the chamber has been seted up to the inside of heating cabinet, place the chamber and correspond with perpendicular hole, place the bottom fixed mounting in chamber has the hot plate, the inside of hot plate is provided with the heating wire, the inside of placing the chamber is provided with the inner bag, clamping device is including the connecting block, the bottom of connecting block and the top fixed connection of lifter plate, the spout has all been seted up to two surveys of connecting block, the inside sliding connection of spout has the slider, one side fixedly connected with connecting plate that the spout was kept away from to the slider, the adjusting screw runs through, fixedly installed clamping plate between the one end that adjusting screw was kept away from to two connecting plates, the inside fixed mounting of clamping plate has a plurality of spring connection arc plates, the spring connection has the clamping plate one end.
Through adopting above-mentioned technical scheme, can be through the hot plate in the three placing chamber respectively with the water heating in the three inner bag to corresponding high temperature denaturation, low temperature annealing (renaturation) and the temperature interval that the moderate temperature extends, place the PCR amplification reaction tube between two arc, adjusting screw, drive the grip block and tighten the PCR amplification reaction tube, spring and the arc in the grip block can better carry out the centre gripping fixedly, place the PCR amplification reaction tube into the inner bag of high temperature denaturation through electric telescopic handle, treat the high temperature denaturation and end, rise the lifter plate, pass through the supporting seat with the PCR amplification reaction tube and change the inner bag top of low temperature annealing, treat the low temperature annealing and end, rise the lifter plate, pass through the supporting seat with the moderate temperature extension inner bag top that the PCR amplification reaction tube changed, rethread electric telescopic handle with the PCR amplification reaction tube place into the moderate temperature extension inner bag top, with this is a circulation, do not need constantly through heating or cooling go on, the temperature change device that can carry out the PCR amplification reaction through the heating and cooling, the variable temperature device can not be fixed to the PCR amplification reaction.
The utility model is further provided with: the top of the heating box is rotationally connected with a box cover through a hinge.
Through adopting above-mentioned technical scheme, the case lid can prevent heat loss when reacting, increases the heat preservation effect.
The utility model is further provided with: one side of the case cover is fixedly provided with a first handle.
Through adopting above-mentioned technical scheme, can conveniently open and close the case lid through first handle.
The utility model is further provided with: and an anti-slip strip is fixedly arranged on one side of the arc-shaped plate, which is far away from the clamping plate.
By adopting the technical scheme, the loosening is prevented when the PCR amplification reaction tube is clamped, and the PCR amplification reaction tube is better clamped and fixed.
The utility model is further provided with: the inside of the inner container is provided with a temperature sensor.
By adopting the technical scheme, the reaction temperature in the three inner containers can be conveniently known, and the reaction can be better detected.
The utility model is further provided with: the inner wall both sides of inner bag are all fixedly connected with rotation axis, the inside of inner bag is provided with the second handle, the both ends of second handle are connected with the rotation axis rotation respectively.
Through adopting above-mentioned technical scheme, can conveniently change the water in inner bag and the inner bag through the second handle, when not using, can rotate, hide the second handle on the inner wall of inner bag.
In summary, the utility model has the following beneficial effects: the water in the three inner containers is heated to the corresponding temperature ranges of high-temperature denaturation, low-temperature annealing (renaturation) and temperature adaptation extension respectively through the heating plates in the three placing cavities, the PCR amplification reaction tube is clamped and fixed through the clamping device, the PCR amplification reaction tube is placed into the inner container of the high-temperature denaturation through the electric telescopic rod, the lifting plate is lifted, the PCR amplification reaction tube is transferred to the upper part of the inner container of the low-temperature annealing through the supporting seat, the PCR amplification reaction tube is placed into the inner container of the low-temperature annealing through the electric telescopic rod, the lifting plate is lifted after the low-temperature annealing is finished, the PCR amplification reaction tube is transferred to the upper part of the temperature adaptation extension inner container through the supporting seat, and the PCR amplification reaction tube is placed into the inner container of the temperature adaptation extension through the electric telescopic rod, so that the PCR amplification reaction tube is circulated, the temperature change is not required to be continuously performed through heating or cooling, the time of heating and cooling is saved, and the PCR amplification reaction tubes with different diameters can be clamped and fixed through the clamping device.
Drawings
FIG. 1 is a schematic three-dimensional structure of the present utility model;
FIG. 2 is a schematic top view of the present utility model;
FIG. 3 is a schematic view of a front cross-sectional structure of the present utility model;
FIG. 4 is a schematic cross-sectional three-dimensional structure of a clamping device according to the present utility model;
FIG. 5 is a schematic diagram showing a sectional front view of the inner container of the heating box according to the present utility model;
fig. 6 is an enlarged schematic view of the structure of the present utility model at a.
In the figure: 1. a case cover; 2. a first handle; 3. a hinge; 4. an arc-shaped plate; 5. a lifting plate; 6. a heating box; 7. an inner container; 8. a connecting block; 9. adjusting a screw; 10. a support base; 11. an electric telescopic rod; 12. a slide block; 13. a chute; 14. a connecting plate; 15. a clamping plate; 16. a spring; 17. a second handle; 18. a temperature sensor; 19. a heating plate; 20. a placement cavity; 21. a rotation shaft; 22. an anti-slip strip; 23. and (5) erecting holes.
Detailed Description
The present utility model will be further described with reference to the drawings in the embodiments of the present utility model.
Referring to fig. 1-5, in the embodiment of the utility model, a reaction tube heating device for PCR amplification comprises a heating box 6, an electric telescopic rod 11 is arranged in the heating box 6, the output end of the electric telescopic rod 11 is fixedly connected with a supporting seat 10, the top of the supporting seat 10 is rotatably connected with a lifting plate 5, three clamping devices are arranged on the top wall of the lifting plate 5, three vertical holes 23 are arranged on the lifting plate 5 corresponding to the clamping devices, three placing cavities 20 are arranged in the heating box 6, the placing cavities 20 correspond to the vertical holes 23, a heating plate 19 is fixedly arranged at the bottom of the placing cavities 20, heating wires are arranged in the heating plate 19, an inner container 7 is arranged in the placing cavities 20, the clamping devices comprise connecting blocks 8, the bottom of the connecting blocks 8 is fixedly connected with the top of the lifting plate 5, the two measurements of the connecting block 8 are provided with sliding grooves 13, the inside of the sliding grooves 13 is connected with sliding blocks 12 in a sliding manner, one side of the sliding blocks 12 far away from the sliding grooves 13 is fixedly connected with connecting plates 14, the inside of the connecting block 8 is connected with adjusting screws 9 in a threaded manner, the adjusting screws 9 penetrate through the connecting block 8, clamping plates 15 are fixedly arranged between one ends of the two connecting plates 14 far away from the adjusting screws 9, a plurality of springs 16 are fixedly arranged in the clamping plates 15, one ends of the springs 16 far away from the clamping plates 15 are fixedly connected with arc plates 4, water in three inner containers 7 is heated to corresponding temperature ranges of high temperature denaturation, low temperature annealing (renaturation) and suitable temperature extension respectively through heating plates 19 in three placing cavities 20, a PCR amplification reaction tube is placed between the two arc plates 4, the adjusting screws 9 are adjusted, the clamping plate 15 is driven to tighten the PCR amplification reaction tube, the spring 16 and the arc plate 4 in the clamping plate 15 can be better clamped and fixed, the PCR amplification reaction tube is placed into the inner container 7 which is denatured at high temperature through the electric telescopic rod 11, the lifting plate 5 is lifted up after the high-temperature denaturation is finished, the PCR amplification reaction tube is transferred to the upper part of the inner container 7 which is annealed at low temperature through the supporting seat 10, the PCR amplification reaction tube is placed into the inner container 7 which is annealed at low temperature through the electric telescopic rod 11, the lifting plate 5 is lifted up after the low-temperature annealing is finished, the PCR amplification reaction tube is transferred to the upper part of the inner container 7 which is stretched at proper temperature through the supporting seat 10, and the PCR amplification reaction tube is placed into the inner container 7 which is stretched at proper temperature through the electric telescopic rod 11, so that the PCR amplification reaction tube is circulated, the temperature change is not needed continuously through heating or cooling, the heating and cooling time is saved, and the PCR amplification reaction tubes with different diameters can be clamped and fixed through the clamping device.
Referring to fig. 1 to 3, in the embodiment of the present utility model, the top of the heating box 6 is rotatably connected with the box cover 1 through the hinge 3, and the box cover 1 can prevent heat loss during the reaction, thereby increasing the heat insulation effect.
Referring to fig. 1 to 3, in the embodiment of the present utility model, a first handle 2 is fixedly installed on one side of the case cover 1, and the case cover 1 is conveniently opened and closed by the first handle 2.
Referring to fig. 4, in the embodiment of the present utility model, an anti-slip strip 22 is fixedly installed on the side of the arc plate 4 far from the clamping plate 15, so as to prevent loosening during clamping the PCR amplification reaction tube, and perform better clamping and fixing on the PCR amplification reaction tube.
Referring to fig. 5, in the embodiment of the present utility model, a temperature sensor 18 is disposed inside the inner container 7, and the model of the temperature sensor 18 is AITM1000S-CTAITM1100S-B, so that the reaction temperatures in the three inner containers 7 can be conveniently known, and the reaction can be better detected.
Referring to fig. 6, in the embodiment of the present utility model, two sides of the inner wall of the inner container 7 are fixedly connected with a rotation shaft 21, a second handle 17 is disposed inside the inner container 7, two ends of the second handle 17 are respectively rotatably connected with the rotation shaft 21, and the second handle 17 can facilitate replacement of the inner container 7 and water in the inner container 7, and can rotate to hide the second handle 17 on the inner wall of the inner container 7 when not in use.
Working principle: the water in the three inner containers 7 is heated to the corresponding temperature ranges of high temperature denaturation, low temperature annealing (renaturation) and temperature adaptation extension through the heating plates 19 in the three placing cavities 20, the PCR amplification reaction tube is clamped and fixed through the clamping device, the PCR amplification reaction tube is placed into the inner containers 7 of the high temperature denaturation through the electric telescopic rod 11, the lifting plate 5 is lifted, the PCR amplification reaction tube is transferred to the upper part of the inner containers 7 of the low temperature annealing through the supporting seat 10 after the high temperature denaturation is finished, the PCR amplification reaction tube is placed into the inner containers 7 of the low temperature annealing through the electric telescopic rod 11, the lifting plate 5 is lifted after the low temperature annealing is finished, the PCR amplification reaction tube is transferred to the upper part of the temperature adaptation extension inner containers 7 through the supporting seat 10, and the PCR amplification reaction tube is placed into the inner containers 7 of the temperature adaptation extension through the electric telescopic rod 11.
The foregoing description is only of the preferred embodiments of the utility model, and all changes and modifications that come within the meaning and range of equivalency of the structures, features and principles of the utility model are therefore intended to be embraced therein.
Claims (6)
1. The utility model provides a PCR amplification is with reaction tube heating device, includes heating cabinet (6), its characterized in that: the inside of heating cabinet (6) is provided with electric telescopic handle (11), the output fixedly connected with supporting seat (10) of electric telescopic handle (11), the top of supporting seat (10) is rotated and is connected with lifter plate (5), three clamping device is installed to the roof of lifter plate (5), three vertical hole (23) have been seted up with the corresponding position of clamping device on lifter plate (5), three chamber (20) of placing have been seted up to the inside of heating cabinet (6), it is corresponding with vertical hole (23) to place chamber (20), the bottom fixed mounting of placing chamber (20) has hot plate (19), the inside of hot plate (19) is provided with the heating wire, the inside of placing chamber (20) is provided with inner bag (7), clamping device is including connecting block (8), the bottom of connecting block (8) and the top fixed connection of lifter plate (5), the spout (13) have all been seted up to two surveys of connecting block (8), the inside sliding connection slider (12) have the screw rod (9) of adjusting screw rod (9) are kept away from to one side, connecting block (9) are connected with screw rod (9), clamping plates (15) are fixedly arranged between one ends, far away from the adjusting screw rods (9), of the two connecting plates (14), a plurality of springs (16) are fixedly arranged in the clamping plates (15), and one ends, far away from the clamping plates (15), of the springs (16) are fixedly connected with arc-shaped plates (4).
2. The reaction tube heating apparatus for PCR amplification according to claim 1, wherein: the top of the heating box (6) is rotationally connected with a box cover (1) through a hinge (3).
3. The reaction tube heating apparatus for PCR amplification according to claim 2, wherein: one side of the box cover (1) is fixedly provided with a first handle (2).
4. The reaction tube heating apparatus for PCR amplification according to claim 1, wherein: and an anti-slip strip (22) is fixedly arranged on one side of the arc-shaped plate (4) far away from the clamping plate (15).
5. The reaction tube heating apparatus for PCR amplification according to claim 1, wherein: the inside of the inner container (7) is provided with a temperature sensor (18).
6. The reaction tube heating apparatus for PCR amplification according to claim 1, wherein: the inner wall both sides of inner bag (7) are all fixedly connected with rotation axis (21), the inside of inner bag (7) is provided with second handle (17), the both ends of second handle (17) are connected with rotation axis (21) rotation respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321532377.2U CN220450203U (en) | 2023-06-15 | 2023-06-15 | Reaction tube heating device for PCR amplification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321532377.2U CN220450203U (en) | 2023-06-15 | 2023-06-15 | Reaction tube heating device for PCR amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220450203U true CN220450203U (en) | 2024-02-06 |
Family
ID=89729847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321532377.2U Active CN220450203U (en) | 2023-06-15 | 2023-06-15 | Reaction tube heating device for PCR amplification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220450203U (en) |
-
2023
- 2023-06-15 CN CN202321532377.2U patent/CN220450203U/en active Active
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