CN210367691U

CN210367691U - Staphylococcus aureus detection device

Info

Publication number: CN210367691U
Application number: CN201920712436.1U
Authority: CN
Inventors: 才秀华
Original assignee: Yaheen Beijing Technology Co ltd
Current assignee: Yaheen Beijing Technology Co ltd
Priority date: 2019-05-17
Filing date: 2019-05-17
Publication date: 2020-04-21
Anticipated expiration: 2029-05-17

Abstract

The utility model discloses a staphylococcus aureus check out test set, including casing, mixed oscillation mechanism and cultivation mechanism, mixed oscillation mechanism be located the inner chamber of casing and with casing swing joint, cultivate the inner chamber that the mechanism is located mixed oscillation mechanism and with mixed oscillation mechanism swing joint, the bottom of mixed oscillation mechanism inner chamber is provided with heating mechanism, heating mechanism's one end runs through to mixed oscillation mechanism's outside. The utility model has the advantages that: can use through the cooperation of mixing oscillation mechanism, training mechanism and heating mechanism and shorten the culture time to the color development step that has reduced staphylococcus aureus reaches short-term test's purpose, structural design is simple reasonable, and it is quick convenient to use, and the operation is with low costs with the use, has very strong practicality, has fine adaptability to the great raw materials of detected quantity, has reduced user's work load, has improved user's work efficiency.

Description

Staphylococcus aureus detection device

Technical Field

The utility model belongs to the technical field of the bacterium detects, especially relate to a staphylococcus aureus check out test set.

Background

At least 20 species of Staphylococcus genus are included. Staphylococcus aureus is an important pathogenic bacterium in humans, causing many serious infections. Typically, Staphylococcus aureus is spherical, about 0.8 μm in diameter, and arranged microscopically in a grape string. Staphylococcus aureus is sporulate-free, flagellar-free, mostly capsular-free, gram-positive. Is a bacterium which is not beneficial to human body.

The staphylococcus aureus detection can make preliminary judgment according to the separation culture, dyeing observation, plasma coagulase test, oxidase test and drug resistance detection of bacteria, such as a fluorescent PCR technology, wherein a fluorescent group is added into a common PCR reaction system, the whole PCR reaction process is detected by utilizing fluorescent signal accumulation, and finally, an unknown template is quantified through a standard curve. Real-timePCR detects the tst gene of staphylococcus aureus, and is an effective monitoring means of molecular epidemiology.

The existing staphylococcus aureus detection has the defects of complex equipment, low use and detection efficiency, inconvenience for quick and simple detection and judgment of the existence of the staphylococcus aureus, and long time is needed especially in the field of food detection.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a staphylococcus aureus check out test set to it is complicated to solve the equipment that current staphylococcus aureus detected and relates to, uses and the inefficiency that detects, is unfavorable for the quick existence of carrying out simple detection judgement staphylococcus aureus that carries on, especially needs long time technical problem in food detection field.

The utility model provides an above-mentioned technical problem's technical scheme as follows: the utility model provides a staphylococcus aureus check out test set, includes casing, mixed oscillation mechanism and cultivates the mechanism, mixed oscillation mechanism be located the inner chamber of casing and with casing swing joint, cultivate the mechanism be located mixed oscillation mechanism's inner chamber and with mixed oscillation mechanism swing joint, the bottom of mixed oscillation mechanism inner chamber is provided with heating mechanism, heating mechanism's one end runs through to mixed oscillation mechanism's outside, the inner chamber fixedly connected with third baffle of casing, a plurality of gas outlets have been seted up on the third baffle, heating mechanism is located the bottom of third baffle, the top and the equal fixedly connected with damping spring in bottom of mixed oscillation mechanism both sides, damping spring keeps away from mixed oscillation mechanism's one end and casing fixed connection.

Preferably, mixed oscillation mechanism includes box, case lid, pull ring, pulley yoke, first pulley and vibrating cylinder, the both sides of box all with damping spring fixed connection, the top swing joint of box has the case lid, the top fixedly connected with pull ring of case lid, the equal fixedly connected with pulley yoke in both sides of bottom half, pulley yoke's inner chamber has first pulley through pivot swing joint, the first pulley groove with first pulley looks adaptation is seted up to the bottom of casing inner chamber, the left side fixedly connected with vibrating cylinder of casing inner chamber, vibrating cylinder's output and box fixed connection.

Preferably, the culture mechanism is including holding dish, first baffle, normal saline ware, enrichment agent ware, mixed ware, chromogenic medium and fly leaf, the both sides of fly leaf are all through second pulley and casing sliding connection, the second pulley groove with second pulley looks adaptation is seted up to the inner chamber of casing, the top of fly leaf is provided with holds the dish, hold two first baffles of two top fixedly connected with of dish, two first baffles will hold the dish and separate into four cavities that the area equals, and four cavities are provided with normal saline ware, enrichment agent ware, mixed ware and chromogenic medium respectively.

Preferably, the recess with holding dish looks adaptation is seted up at the top of fly leaf, two first baffles become the cross, the top of holding dish is provided with the recess with normal saline ware, enrichment agent ware, mixed ware and color development culture medium looks adaptation, the equal fixedly connected with in both sides at fly leaf top rotates the handrail, the surface setting and the anti-skidding line of rotatory handrail.

Preferably, heating mechanism includes heating plate, second baffle, filter screen, fan and air-supply line, the bottom fixed mounting who mixes the oscillation mechanism inner chamber has the heating plate, the bottom fixedly connected with second baffle of third baffle, the bottom and the box fixed connection of second baffle, one side fixed mounting of second baffle has the filter screen, the rear side fixed mounting of box inner chamber has the fan, the air outlet of fan passes through hose and second baffle intercommunication, the air intake intercommunication of fan has the air-supply line, the one end that the fan was kept away from to the air-supply line runs through to the outside of box.

1. The utility model has the advantages that: can use through the cooperation of mixing oscillation mechanism, training mechanism and heating mechanism and shorten the culture time to the color development step that has reduced staphylococcus aureus reaches short-term test's purpose, structural design is simple reasonable, and it is quick convenient to use, and the operation is with low costs with the use, has very strong practicality, has fine adaptability to the great raw materials of detected quantity, has reduced user's work load, has improved user's work efficiency.

2. The utility model discloses a setting of case lid can play the sealed effect of box, and through the setting of pull ring, convenient to use person takes off and installs the case lid, uses through the cooperation of pulley yoke and first pulley, vibrates about can being convenient for the box for the more smooth of box motion avoids appearing the condition that the card went up, is favorable to cultivateing the mixing of in-process culture material.

3. The utility model discloses a plurality of culture dishes can be placed in holding tray's setting. Convenient to use person takes and cultivates, has improved user's work efficiency, has shortened the process of cultivateing the detection, has very strong practicality, through the setting of first baffle, can separate into four regions with holding the dish, has avoided the appearance of every regional mutual interference pollution condition, is favorable to improving the accuracy that detects for detect structure accuracy more.

4. The utility model discloses a setting of heating plate and fan can become the hot-blast inner chamber that blows to the box with outside cold wind, controls the temperature of box inner chamber then, reaches the ambient temperature of best cultivation, has shortened the time of cultivateing the detection, has guaranteed the normal clear of cultivateing the testing process.

5. The utility model discloses a damping spring's setting can play the effect of shock attenuation buffering to the box for box motion more stable, through the setting of second pulley and rotatory handrail, can be convenient for the user through the rotatory fly leaf of rotatory handrail, the material that is convenient for to use in the process of will cultivateing carries out mixed oscillation, has improved mixed oscillation's efficiency.

Drawings

The above and/or other aspects and advantages of the present invention will become more apparent and more readily appreciated from the detailed description taken in conjunction with the following drawings, which are given by way of illustration only and not by way of limitation, wherein:

FIG. 1 is a schematic structural diagram of an embodiment of a Staphylococcus aureus detection apparatus of the present invention;

FIG. 2 is a schematic perspective view of a tray of an embodiment of a Staphylococcus aureus detection apparatus;

fig. 3 is an enlarged schematic view of a in fig. 1 according to the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

1. a housing; 101. a first pulley groove; 102. a second pulley groove; 2. a mixing and oscillating mechanism; 201. a box body; 202. a box cover; 203. a pull ring; 204. a pulley yoke; 205. a first pulley; 206. a vibration cylinder; 3. a culture mechanism; 301. a holding tray; 302. a first separator; 303. a saline dish; 304. a bacteria increasing agent vessel; 305. a mixing vessel; 306. a chromogenic medium; 307. a movable plate; 4. a heating mechanism; 401. a heating plate; 402. a second separator; 403. a filter screen; 404. a fan; 405. an air inlet pipe; 5. a damping spring; 6. a second pulley; 7. a third partition plate; 8. an air outlet; 9. the armrest is rotated.

Detailed Description

Hereinafter, an embodiment of a staphylococcus aureus detecting apparatus of the present invention will be described with reference to the accompanying drawings.

The embodiments described herein are specific embodiments of the present invention, and are intended to be illustrative of the concepts of the present invention, which are intended to be illustrative and exemplary, and should not be construed as limiting the scope of the embodiments of the present invention. In addition to the embodiments described herein, those skilled in the art will be able to employ other technical solutions which are obvious based on the disclosure of the claims and the specification of the present application, and these technical solutions include technical solutions which make any obvious replacement or modification for the embodiments described herein.

The drawings in the present specification are schematic views to assist in explaining the concept of the present invention, and schematically show the shapes of the respective portions and the mutual relationships thereof. It should be noted that for the sake of clarity in showing the structures of the various components of the embodiments of the present invention, the drawings are not drawn to the same scale. Like reference numerals are used to denote like parts.

Fig. 1-3 show an embodiment of a staphylococcus aureus detecting apparatus of the present invention, which comprises a housing 1, a mixed oscillation mechanism 2 and a culturing mechanism 3, wherein the mixed oscillation mechanism 2 is located in an inner cavity of the housing 1 and movably connected with the housing 1, the mixed oscillation mechanism 2 comprises a box 201, a box cover 202, a pull ring 203, a pulley frame 204, a first pulley 205 and a vibrating cylinder 206, both sides of the box 201 are fixedly connected with a damping spring 5, the top of the box 201 is movably connected with the box cover 202, the top of the box cover 202 is fixedly connected with the pull ring 203, both sides of the bottom of the box 201 are fixedly connected with the pulley frame 204, the inner cavity of the pulley frame 204 is movably connected with the first pulley 205 through a rotating shaft, the bottom of the inner cavity of the housing 1 is provided with a first pulley groove 101 adapted to the first pulley 205, the left side of the inner cavity of the housing 1 is fixedly connected with the vibrating, the output end of the vibrating cylinder 206 is fixedly connected with the box body 201, the box cover 202 is arranged to play a role of sealing the box body 201, the box cover 202 is convenient for a user to take down and install through the arrangement of the pull ring 203, the box body 201 can vibrate left and right through the matching use of the pulley frame 204 and the first pulley 205, so that the movement of the box body 201 is smoother, the occurrence of blocking is avoided, and the mixing of culture substances in the culture process is facilitated, the culture mechanism 3 is positioned in the inner cavity of the mixed oscillation mechanism 2 and is movably connected with the mixed oscillation mechanism 2, the culture mechanism 3 comprises a holding plate 301, a first partition plate 302, a physiological saline dish 303, an agent enrichment dish 304, a mixed dish 305, a chromogenic culture medium 306 and a movable plate 307, the two sides of the movable plate 307 are both connected with the shell body 1 through the second pulley 6 in a sliding manner, the inner cavity of the shell body 1 is provided with a second pulley groove 102 matched with the, the top of fly leaf 307 is provided with holding tray 301, hold two first baffles 302 of two top fixedly connected with of tray 301, two first baffles 302 will hold tray 301 and separate into four cavities that the area is equal, four cavities are provided with normal saline ware 303 respectively, the agent ware 304 that increases, mix ware 305 and color development culture medium 306, the recess with holding tray 301 looks adaptation is seted up at the top of fly leaf 307, two first baffles 302 become the cross, the top that holds tray 301 is provided with and normal saline ware 303, the agent ware 304 that increases, the recess of mixing ware 305 and color development culture medium 306 looks adaptation, the equal fixedly connected with in both sides at fly leaf 307 top rotates handrail 9, the surface setting and the anti-skidding line of rotatory handrail 9, setting through holding tray 301, can place a plurality of culture dishes. The utility model has the advantages of convenient use and cultivation for users, improved working efficiency for users, shortened cultivation and detection process, strong practicability, the arrangement of the first clapboard 302 can divide the containing disc 301 into four areas, avoiding the occurrence of mutual interference and pollution of each area, being beneficial to improving detection accuracy, and enabling detection structure to be more accurate, the bottom of the inner cavity of the mixed oscillation mechanism 2 is provided with the heating mechanism 4, the heating mechanism 4 comprises a heating sheet 401, a second clapboard 402, a filter screen 403, a fan 404 and an air inlet pipe 405, the bottom of the inner cavity of the mixed oscillation mechanism 2 is fixedly provided with the heating sheet 401, the bottom of the third clapboard 7 is fixedly connected with the second clapboard 402, the bottom of the second clapboard 402 is fixedly connected with the box body 201, one side of the second clapboard 402 is fixedly provided with the filter screen 403, the rear side of the inner cavity of the box body 201 is fixedly provided with the fan 404, an air outlet of the fan 404 is communicated with the second partition plate 402 through a hose, an air inlet of the fan 404 is communicated with an air inlet pipe 405, one end, far away from the fan 404, of the air inlet pipe 405 penetrates to the outside of the box body 201, through the arrangement of the heating plate 401 and the fan 404, external cold air can be changed into hot air to be blown to the inner cavity of the box body 201, the temperature of the inner cavity of the box body 201 is controlled, the optimal cultivation environment temperature is achieved, cultivation detection time is shortened, normal operation of cultivation detection process is guaranteed, one end of the heating mechanism 4 penetrates to the outside of the hybrid oscillation mechanism 2, the inner cavity of the shell 1 is fixedly connected with a third partition plate 7, a plurality of air outlets 8 are formed in the third partition plate 7, the heating mechanism 4 is located at the bottom of the third partition plate 7, damping springs 5 are fixedly connected to the top and the bottom of the two sides of the hybrid oscillation mechanism 2, and through the arrangement, make more stable of box 201 motion, through the setting of second pulley 6 and rotatory handrail 9, can be convenient for the user through rotatory fly leaf 307 of rotatory handrail 9, be convenient for carry out the mixed oscillation with the material that cultivates the in-process and use, improved mixed oscillation's efficiency, damping spring 5 keeps away from mixed oscillation mechanism 2's one end and casing 1 fixed connection.

The utility model discloses in: the heater chip 401 is of type YK-BW, the fan 404 is of type FS127, and the vibrating cylinder 206 is of type SC 160.

The utility model discloses during the use, S1: and (3) enrichment: adding a sample to be detected into a normal saline dish 303 containing normal saline to prepare a sample homogenizing solution with the sample mass concentration of 20%; according to the following steps: mixing the enrichment agent and water according to the mass ratio of 200, and dissolving the mixture in an enrichment agent vessel 304 to obtain enrichment liquid; mixing the sample homogenizing solution and enrichment solution according to the proportion of 1: 5, uniformly mixing the mixture in a mixing vessel 305, controlling the temperature to be 37 ℃, putting a thermometer into an inner cavity of the mixing and oscillating mechanism 2, starting a heating plate 401, blowing cold air onto the heating plate 401 by a fan 404 through an air inlet pipe 405 to become hot air, blowing the hot air to the top of a box body 201 through an air outlet 8, enabling the top of the box body 201 to reach a proper temperature, starting a vibration cylinder 206, driving the box body 201 to vibrate left and right by an output end of the vibration cylinder 206, driving a first pulley 205 to slide at the bottom of a shell 1 by the box body 201, driving a culture mechanism 3 in the inner cavity of the mixing and oscillating mechanism 2 to vibrate by the box body 201, and performing vibration culture for 5 hours to obtain a sample enrichment;

s2: performing heat treatment to ensure that the liquid level height of the enrichment liquid is 0.7cm, raising the temperature to ensure that the temperature of the inner cavity of the box body 201 is about 65 ℃, and continuously culturing for 1.5 hours;

s3: culturing and developing: and uniformly coating 1mL of the enrichment fluid after heat treatment in a chromogenic medium 306, controlling the temperature to be about 40 ℃, continuously culturing for 19 hours, observing the color change in the chromogenic medium 306, wherein if pink halo appears, the existence of staphylococcus aureus in the sample to be detected is indicated, and if the pink halo does not appear, the absence of staphylococcus aureus is indicated, so that the aim of quickly detecting the staphylococcus aureus is fulfilled.

The technical features disclosed above are not limited to the combinations with other features disclosed, and other combinations between the technical features can be performed by those skilled in the art according to the purpose of the invention to achieve the aim of the invention.

Claims

1. The staphylococcus aureus detection device comprises a shell (1), a mixed oscillation mechanism (2) and a culture mechanism (3), and is characterized in that the mixed oscillation mechanism (2) is positioned in an inner cavity of the shell (1) and movably connected with the shell (1), the culture mechanism (3) is positioned in the inner cavity of the mixed oscillation mechanism (2) and movably connected with the mixed oscillation mechanism (2), a heating mechanism (4) is arranged at the bottom of the inner cavity of the mixed oscillation mechanism (2), one end of the heating mechanism (4) penetrates through the outer part of the mixed oscillation mechanism (2), a third partition plate (7) is fixedly connected with the inner cavity of the shell (1), a plurality of air outlets (8) are formed in the third partition plate (7), the heating mechanism (4) is positioned at the bottom of the third partition plate (7), and damping springs (5) are fixedly connected with the top and the bottom of the two sides of the mixed oscillation mechanism (2), and one end of the damping spring (5) far away from the mixed oscillation mechanism (2) is fixedly connected with the shell (1).

2. The staphylococcus aureus detecting device according to claim 1, wherein the mixed oscillating mechanism (2) comprises a box body (201), a box cover (202), a pull ring (203), a pulley frame (204), a first pulley (205) and a vibrating cylinder (206), both sides of the box body (201) are fixedly connected with a damping spring (5), the top of the box body (201) is movably connected with the box cover (202), the top of the box cover (202) is fixedly connected with the pull ring (203), both sides of the bottom of the box body (201) are fixedly connected with the pulley frame (204), an inner cavity of the pulley frame (204) is movably connected with the first pulley (205) through a rotating shaft, the bottom of the inner cavity of the housing (1) is provided with a first pulley groove (101) matched with the first pulley (205), and the left side of the inner cavity of the housing (1) is fixedly connected with the vibrating cylinder (206), the output end of the vibration cylinder (206) is fixedly connected with the box body (201).

3. The staphylococcus aureus detection device according to claim 2, wherein the culture mechanism (3) comprises a containing tray (301), a first partition plate (302), a saline dish (303), a bactericide dish (304), a mixing dish (305), a chromogenic medium (306) and a movable plate (307), wherein two sides of the movable plate (307) are slidably connected with the housing (1) through a second pulley (6), a second pulley groove (102) matched with the second pulley (6) is formed in an inner cavity of the housing (1), the containing tray (301) is arranged at the top of the movable plate (307), two first partition plates (302) are fixedly connected to the top of the containing tray (301), the containing tray (301) is divided into four chambers with equal areas by the two first partition plates (302), and the saline dishes (303) and the movable plate (307) are respectively arranged in the four chambers, A bacteria increasing agent dish (304), a mixing dish (305) and a chromogenic medium (306).

4. The staphylococcus aureus detection device according to claim 3, wherein a groove matched with the containing tray (301) is formed in the top of the movable plate (307), the two first partition plates (302) are cross-shaped, the groove matched with the saline container (303), the bacteria increasing container (304), the mixing container (305) and the chromogenic medium (306) is formed in the top of the containing tray (301), rotary handrails (9) are fixedly connected to two sides of the top of the movable plate (307), and anti-skid patterns are formed in the surface of each rotary handrail (9).

5. Staphylococcus aureus apparatus according to claim 2, the heating mechanism (4) comprises a heating sheet (401), a second clapboard (402), a filter screen (403), a fan (404) and an air inlet pipe (405), the bottom of the inner cavity of the mixing and oscillating mechanism (2) is fixedly provided with a heating plate (401), the bottom of the third clapboard (7) is fixedly connected with a second clapboard (402), the bottom of the second clapboard (402) is fixedly connected with the box body (201), a filter screen (403) is fixedly arranged on one side of the second clapboard (402), a fan (404) is fixedly arranged on the rear side of the inner cavity of the box body (201), the air outlet of the fan (404) is communicated with the second clapboard (402) through a hose, an air inlet of the fan (404) is communicated with an air inlet pipe (405), and one end, far away from the fan (404), of the air inlet pipe (405) penetrates through the outer portion of the box body (201).