CN106771116B - A kind of strainer antianaphylaxis detection method and device - Google Patents
A kind of strainer antianaphylaxis detection method and device Download PDFInfo
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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Abstract
The invention discloses a kind of strainer antianaphylaxis detection methods to include the following steps for detecting the antianaphylaxis of air cleaning unit Inner filter net：S100:The strainer is cut to 50 millimeters of diameter of print A；S200：Sterilization treatment is carried out to print A, print B and print C respectively；S300：Print A is placed in 250 milliliters of sterile triangular flask A；S400：The dilution of dye allergen solution is dripped on print A and print B；S500：Sterile triangular flask A, sterile triangular flask B and sterile triangular flask C are placed in the space that environment temperature is 25 degrees Celsius and stand 1 hour；S600：10 milliliters of anaphylactogen extracting solutions are added into sterile triangular flask A, sterile triangular flask B and sterile triangular flask C respectively.The present invention also provides a kind of strainer antianaphylaxis detection devices.The present invention is capable of detecting when result of the antianaphylaxis of sample strainer compared with non-woven fabrics using non-woven fabrics as benchmark.
Description
Technical field
The present invention relates to a kind of strainer antianaphylaxis detection method and device, belong to household electrical appliance inspection, detection field.
Background technique
With the continuous deterioration of environment, air quality problems increasingly attract people's attention, such as indoor air-conditioning, sky
Gas purifier etc. plays the role of improving air quality.Its principle for purifying air is all largely to be carried out by strainer to air
Filtering, the impurity being mingled in air is separated, so, filter screen plays the role of impurity separation, also results in having big
The impurity of amount is attached on filter screen, causes to breed a large amount of anaphylactogens on filter screen.The filter screen of unlike material is to anaphylactogen
Resistivity is different, can often be cleaned, be sterilized to filter screen in daily life, cannot be led to by the strainer that anaphylactogen corrodes
It is continued to use after crossing simple cleaning, disinfection, but has to the strainer more renewed, but filter screen supports anaphylactogen
Anti- ability, so being badly in need of a kind of method for being able to detect filter screen antianaphylaxis, cannot be that air is net by visually being recognized
Change equipment and selects the higher strainer of antianaphylaxis.
Summary of the invention
The object of the present invention is to provide a kind of strainer antianaphylaxis detection methods, are able to detect strainer in household electrical appliance
Antianaphylaxis, while the present invention also provides the detection devices that the detection method is used.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
A kind of strainer antianaphylaxis detection method, for detecting the antianaphylaxis of air cleaning unit Inner filter net, including such as
Lower step：
S100:The strainer is cut to 50 millimeters of diameter of print A；Taking two diameters is 50 millimeters of non-woven fabrics print,
It is denoted as print B and print C；
S200：Sterilization treatment is carried out to print A, print B and print C respectively, this step is primarily to later analysis
One fair basis is provided, sterilizes first to three prints, prevents parasitism on three prints from having anaphylactogen, if print
Upper parasitism has anaphylactogen, will affect subsequent comparison, judgement；
S300：Print A is placed in 250 milliliters of sterile triangular flask A, print B is placed in sterile the three of 250 milliliters
In the bottle B of angle, print C is placed in 250 milliliters of sterile triangular flask C；
S400：On print A and print B drop dye allergen solution dilution, with sealed membrane by sterile triangular flask A,
The bottle sealing of sterile triangular flask B and sterile triangular flask C；
S500：It is 25 degrees Celsius that sterile triangular flask A, sterile triangular flask B and sterile triangular flask C, which are placed in environment temperature,
Space in stand 1 hour, this detection method select dust mite mite body as anaphylactogen, dust mite mite body is grown most at 25 degrees Celsius
Be it is rapid, help to shorten detection cycle, save the time, and standing one hour is because overlong time then will lead to anaphylactogen number
Amount is too many, too intensive, the difficulty that the increase later period compares, judges；
S600：10 milliliters of anaphylactogens are added into sterile triangular flask A, sterile triangular flask B and sterile triangular flask C respectively to extract
Liquid is to carry out concussion 18 hours in 25 degrees Celsius of space with the revolving speed of 200r/min in environment temperature, by many experiments and
It accurately calculates, if it is too fast, anaphylactogen and solution height can be mixed, it is difficult to which environment temperature is worked as in the separation for carrying out the later period
It is 25 degrees Celsius, with best separating effect when revolving speed is 200r/min；
S700：Supernatant in sterile triangular flask A, sterile triangular flask B and sterile triangular flask C is extracted respectively, and is detected respectively
Allergen content in corresponding supernatant, the anaphylaxis that can analyze anaphylactogen is compared to the print in three triangular flasks
It can be with antianaphylaxis of the strainer relative to non-woven fabrics, comparison basis of this detection method using non-woven fabrics as detection.
As one of embodiment, the step S200 further includes following step：If print A can be resistant to height
Warm high pressure is sterilized 20 minutes using 121 degrees Celsius；If print A does not tolerate high temperature and pressure, using ultraviolet sterilization 30 minutes.
Alternatively embodiment, the step S200 further includes following step：If print A can be resistant to height
Warm high pressure is sterilized 20 minutes using 121 degrees Celsius；If print A does not tolerate high temperature and pressure, with aseptic water washing 11 minutes, dry in the air
It is dry.
In a kind of strainer antianaphylaxis detection method above-mentioned, the step S700 further includes following step：By enzyme-linked
Immunization detects the content of anaphylactogen in the supernatant.
In a kind of strainer antianaphylaxis detection method above-mentioned, the preparation method of the allergen solution includes following steps
Suddenly：
B100：Dust mite mite body is put into mortar；
B200：Liquid nitrogen is added in mortar, so that liquid nitrogen was not had dust mite mite body, mortar is rested on into 15-20 after liquid nitrogen volatilization
It is kept for 10 minutes in degree Celsius environment, the process that liquid nitrogen is a multigelation is added, it is complete to be conducive to clasmatosis, temperature mistake
It is low to be unfavorable for liquid nitrogen volatilization, the excessively high destructible protein stability of temperature；
B300：It repeats step B200 tetra- times, grinds dust mite mite body 10 minutes；
B400：Dust mite mite body after grinding is placed in plastic centrifuge tube, by 0.1g：1mlCoca alkalinity extracts liquid proportional
Alkaline extracting solution is added, plastic centrifuge tube is placed in the space that environment temperature is 4 degrees Celsius and is placed 48-72 hours, institute
It is protein denaturization in order to prevent to be placed in 4 degrees Celsius of space；
B500：It is stirred 4 hours with magnetic stirring apparatus or oscillator within every 24 hours, is filtered out with double-layer filter paper during the extraction process
Extracting solution is poured into bag filter by polypide and impurity in solution, and every 4 hours replace a bag filter, can extract so more
Completely；
B600：Degerming is carried out to filtered anaphylactogen, protein content is then measured by sampling, and mark；
B700：Anaphylactogen is placed in subzero 20 degrees Celsius of environment and is saved, this be in order to maintain protein structure, if
Temperature is excessively high, and protein is apt to deteriorate.
In a kind of strainer antianaphylaxis detection method above-mentioned, the component of the Coca alkalinity extracting solution is：Sodium chloride, carbon
Sour hydrogen sodium, phenol and water, each component weight ratio are followed successively by：5：2.75：4：1000；Sodium chloride, sodium bicarbonate and phenol are put into
It in water, stirs evenly, is then placed in 4 degrees Celsius of environment, sodium chloride provides liquid environment concentration, and sodium bicarbonate provides alkalescent ring
Border, phenol anti-corrosion, water prepare solution base substance.
The strainer antianaphylaxis detection device that a kind of strainer antianaphylaxis detection method uses, including ontology and close
Capping, sealing cover are located at the top of ontology, and sealing cover can cover the top of ontology, and ontology is equipped with workbench, workbench
Lower section has independent sealing temperature control compartment A and sealing temperature control compartment B, is equipped with heat generating device and cooling device in workbench；Fever
The port A communication seals temperature control compartment A of device, the port B communication seals temperature control compartment B of heat generating device, the port C connection of cooling device
Seal temperature control compartment A, the port D communication seals temperature control compartment B of cooling device；Seal temperature control compartment A and sealing temperature control compartment B by partition every
It opens, partition runs through the upper and lower part of workbench, is respectively equipped at least three rotating bases on the workbench of partition two sides, rotates
Pedestal is equipped with the fixing seat for fixed triangle bottle；Workbench be equipped with control system, control system respectively with rotating base,
Heat generating device and cooling device electrical connection.
In strainer antianaphylaxis detection device above-mentioned, it is equipped with the first temperature sensor in the sealing temperature control compartment A, first
Temperature sensor and control system electrical connection；It seals and is equipped with second temperature sensor, second temperature sensor in temperature control compartment B
It is electrically connected with the control system；Control system has temperature display panel.
In strainer antianaphylaxis detection device above-mentioned, partition has the first airflow channel and the second airflow channel, sealing
Temperature control compartment A is connected to by the first airflow channel with the workbench upper space of partition side, and sealing temperature control compartment B passes through the second air-flow
Channel is connected to the workbench upper space of the partition other side.
Compared with prior art, the present invention is capable of detecting when the antianaphylaxis of sample strainer using non-woven fabrics as benchmark
It is compared with non-woven fabrics as a result, detected using this method to strainer, more intuitive can observe the antiallergy of strainer
Property, consequently facilitating picking out more good screen material, have the function of to the optimized Selection of indoor household electric appliance positive.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of embodiment of the invention；
Fig. 2 is the structural schematic diagram for opening the ontology after sealing cover.
Appended drawing reference：1- workbench, 2- rotating base, 3- fixing seat, the first airflow channel of 4-, the second airflow channel of 5-,
6- ontology, 7- seal temperature control compartment B, 8- second temperature sensor, the port 9- D, 10- cooling device, the first temperature of the port 11- C, 12-
Sensor is spent, 13- seals temperature control compartment A, 14- control system, the port 15- A, 16- heat generating device, the port 17- B.
The present invention is further illustrated with reference to the accompanying drawings and detailed description.
Specific embodiment
The embodiment of the present invention 1：A kind of strainer antianaphylaxis detection method, for detecting air cleaning unit Inner filter net
Antianaphylaxis includes the following steps：
S100:The strainer is cut to 50 millimeters of diameter of print A；Taking two diameters is 50 millimeters of non-woven fabrics print,
It is denoted as print B and print C；
S200：Sterilization treatment is carried out to print A, print B and print C respectively, if print A can be resistant to high temperature and pressure,
It is sterilized 20 minutes using 121 degrees Celsius；If print A does not tolerate high temperature and pressure, with aseptic water washing 11 minutes, dry；
S300：Print A is placed in 250 milliliters of sterile triangular flask A, print B is placed in sterile the three of 250 milliliters
In the bottle B of angle, print C is placed in 250 milliliters of sterile triangular flask C；
S400：On print A and print B drop dye allergen solution dilution, with sealed membrane by sterile triangular flask A,
The bottle sealing of sterile triangular flask B and sterile triangular flask C；
S500：It is 25 degrees Celsius that sterile triangular flask A, sterile triangular flask B and sterile triangular flask C, which are placed in environment temperature,
Space in stand 1 hour；
S600：10 milliliters of anaphylactogens are added into sterile triangular flask A, sterile triangular flask B and sterile triangular flask C respectively to extract
Liquid carries out concussion 18 hours in the space that environment temperature is 25 degrees Celsius with the revolving speed of 200r/min；
S700：Supernatant in sterile triangular flask A, sterile triangular flask B and sterile triangular flask C is extracted respectively, and is detected respectively
Allergen content in corresponding supernatant, the content of anaphylactogen in the supernatant is detected by enzyme-linked immunization.
Wherein, step S300~S700 is to be operated in 5 DEG C~35 DEG C in environment temperature, and the precision of environment temperature is wanted
Control is within ± 1 DEG C；Entire detection method requires control surrounding air relative humidity 10%~85%, and the precision of humidity will be controlled
System is within ± 5%.
Embodiment 2：A kind of strainer antianaphylaxis detection method, for detecting the antiallergy of air cleaning unit Inner filter net
Property, include the following steps：
S100:The strainer is cut to 50 millimeters of diameter of print A；Taking two diameters is 50 millimeters of non-woven fabrics print,
It is denoted as print B and print C；
S200：Sterilization treatment is carried out to print A, print B and print C respectively, if print A can be resistant to high temperature and pressure,
It is sterilized 20 minutes using 121 degrees Celsius；If print A does not tolerate high temperature and pressure, using ultraviolet sterilization 30 minutes；
S300：Print A is placed in 250 milliliters of sterile triangular flask A, print B is placed in sterile the three of 250 milliliters
In the bottle B of angle, print C is placed in 250 milliliters of sterile triangular flask C；
S400：On print A and print B drop dye allergen solution dilution, with sealed membrane by sterile triangular flask A,
The bottle sealing of sterile triangular flask B and sterile triangular flask C；
S500：It is 25 degrees Celsius that sterile triangular flask A, sterile triangular flask B and sterile triangular flask C, which are placed in environment temperature,
Space in stand 1 hour；
S600：10 milliliters of anaphylactogens are added into sterile triangular flask A, sterile triangular flask B and sterile triangular flask C respectively to extract
Liquid carries out concussion 18 hours in the space that environment temperature is 25 degrees Celsius with the revolving speed of 200r/min；
S700：Supernatant in sterile triangular flask A, sterile triangular flask B and sterile triangular flask C is extracted respectively, and is detected respectively
Allergen content in corresponding supernatant, the content of anaphylactogen in the supernatant is detected by enzyme-linked immunization.
Wherein, step S300~S700 is to be operated in 5 DEG C~35 DEG C in environment temperature, and the precision of environment temperature is wanted
Control is within ± 1 DEG C；Entire detection method requires control surrounding air relative humidity 10%~85%, and the precision of humidity will be controlled
System is within ± 5%.
Embodiment 3：1. a kind of strainer antianaphylaxis detection method, for detecting the antiallergy of air cleaning unit Inner filter net
Property, which is characterized in that include the following steps：
S100:The strainer is cut to 50 millimeters of diameter of print A；Taking two diameters is 50 millimeters of non-woven fabrics print,
It is denoted as print B and print C；
S200：Sterilization treatment is carried out to print A, print B and print C respectively, if print A can be resistant to high temperature and pressure,
It is sterilized 20 minutes using 121 degrees Celsius；If print A does not tolerate high temperature and pressure, using ultraviolet sterilization 30 minutes；
S300：Print A is placed in 250 milliliters of sterile triangular flask A, print B is placed in sterile the three of 250 milliliters
In the bottle B of angle, print C is placed in 250 milliliters of sterile triangular flask C；
S400：On print A and print B drop dye allergen solution dilution, with sealed membrane by sterile triangular flask A,
The bottle sealing of sterile triangular flask B and sterile triangular flask C；
S500：It is 25 degrees Celsius that sterile triangular flask A, sterile triangular flask B and sterile triangular flask C, which are placed in environment temperature,
Space in stand 1 hour；
S600：10 milliliters of anaphylactogens are added into sterile triangular flask A, sterile triangular flask B and sterile triangular flask C respectively to extract
Liquid carries out concussion 18 hours in the space that environment temperature is 25 degrees Celsius with the revolving speed of 200r/min；
S700：Supernatant in sterile triangular flask A, sterile triangular flask B and sterile triangular flask C is extracted respectively, and is detected respectively
Allergen content in corresponding supernatant, the content of anaphylactogen in the supernatant is detected by enzyme-linked immunization.
In a kind of strainer antianaphylaxis detection method above-mentioned, the preparation method of the allergen solution includes following steps
Suddenly：
B100：Dust mite mite body is put into mortar；
B200：Liquid nitrogen is added in mortar, so that liquid nitrogen was not had dust mite mite body, mortar is rested on into 15-20 after liquid nitrogen volatilization
It is kept for 10 minutes in degree Celsius environment；
B300：It repeats step B200 tetra- times, grinds dust mite mite body 10 minutes；
B400：Dust mite mite body after grinding is placed in plastic centrifuge tube, by 0.1g：1mlCoca alkalinity extracts liquid proportional
Alkaline extracting solution is added, plastic centrifuge tube is placed in the space that environment temperature is 4 degrees Celsius and is placed 48-72 hours；Specifically
, the component of the Coca alkalinity extracting solution is：Sodium chloride, sodium bicarbonate, phenol and water, each component weight ratio are followed successively by：5：
2.75：4：1000；Into the water by sodium chloride, sodium bicarbonate and phenol, it stirs evenly, is then placed in 4 degrees Celsius of environment；
B500：It is stirred 4 hours with magnetic stirring apparatus or oscillator within every 24 hours, is filtered out with double-layer filter paper during the extraction process
Extracting solution is poured into bag filter by polypide and impurity in solution, and every 4 hours replace a bag filter；
B600：Degerming is carried out to filtered anaphylactogen, protein content is then measured by sampling, and mark；
B700：Anaphylactogen is placed in subzero 20 degrees Celsius of environment and is saved.
Wherein, step S300~S700 is to be operated in 5 DEG C~35 DEG C in environment temperature, and the precision of environment temperature is wanted
Control is within ± 1 DEG C；Entire detection method requires control surrounding air relative humidity 10%~85%, and the precision of humidity will be controlled
System is within ± 5%.
The strainer antianaphylaxis detection device that detection method described in above embodiments is used, including ontology 6 and sealing cover, this
Body 6 is equipped with workbench 1, and the lower section of workbench 1 has independent sealing temperature control compartment A13 and seals temperature control compartment B7, in workbench 1
Heat generating device 16 and cooling device 10 are installed；The port A15 communication seals temperature control compartment A13 of heat generating device 16, heat generating device 16
Port B17 communication seals temperature control compartment B7, the port C11 communication seals temperature control compartment A13 of cooling device 10, the end of cooling device 10
Mouth D9 communication seals temperature control compartment B7；Sealing temperature control compartment A13 and sealing temperature control compartment B7 are separated by partition, and partition runs through workbench 1
Upper and lower part, at least three rotating bases 2 are respectively equipped on the workbench 1 of partition two sides, rotating base 2 is equipped with and is used for
The fixing seat 3 of fixed triangle bottle；Workbench 1 is equipped with control system 14, and control system 14 is filled with rotating base 2, fever respectively
Set 16 and the electrical connection of cooling device 10.
The first temperature sensor 12, the first temperature sensor 12 and control system are equipped in the sealing temperature control compartment A13
14 electrical connection of system；It seals and is equipped with second temperature sensor 8, second temperature sensor 8 and the control system 14 in temperature control compartment B7
Electrical connection；Control system 14 has temperature display panel.
Partition has the first airflow channel 4 and the second airflow channel 5, and sealing temperature control compartment A13 passes through 4 He of the first airflow channel
1 upper space of workbench of partition side is connected to, and sealing temperature control compartment B7 passes through the work of the second airflow channel 5 and the partition other side
The connection of 1 upper space of platform.
After sealing cover covers, the workbench 1 below sealing cover forms two mutually independent spaces, and two mutually indepedent
Space be separated by partition, this is to make two separate spaces be in different temperatures.Fixing seat 3 is used for fixed triangle
Bottle, rotating base 2 complete centrifugation, the separation of solution for driving triangular flask to rotate.It can be controlled by operation control system 14
Rotating base 2 rotates, and heat generating device 16 can also be controlled by operation control system 14 and cooling device 10 is run.The present apparatus can
To build the different detection environment of two temperature, the different detection environment of two temperature shares a set of heat generating device 16 and cooling dress
Set 10.Pass through the first temperature sensor 12 and the monitoring of second temperature sensor 8 sealing temperature control compartment A13 and the temperature for sealing temperature control compartment B7
Degree, and shown by the display panel of control system 14, meanwhile, control system 14 also has timing module, can pass through
Operation control system 14 customizes the working time of temperature pattern and rotating base 2 in advance.
Claims (3)
1. a kind of strainer antianaphylaxis detection device, which is characterized in that including ontology (6) and sealing cover, ontology (6) is equipped with work
Make platform (1), the lower section of workbench (1) has independent sealing temperature control compartment A (13) and sealing temperature control compartment B (7), and workbench (1) is interior
Heat generating device (16) and cooling device (10) are installed；Port A (15) communication seals temperature control compartment A (13) of heat generating device (16),
Port B (17) communication seals temperature control compartment B (7) of heat generating device (16), port C (11) communication seals temperature control of cooling device (10)
Storehouse A (13), port D (9) communication seals temperature control compartment B (7) of cooling device (10)；Seal temperature control compartment A (13) and sealing temperature control compartment B
(7) be separated by partition, partition runs through the upper and lower part of workbench (1), be respectively equipped on the workbench (1) of partition two sides to
Few three rotating bases (2), rotating base (2) are equipped with the fixing seat (3) for fixed triangle bottle；Workbench (1) is equipped with
Control system (14), control system (14) are electrically connected with rotating base (2), heat generating device (16) and cooling device (10) respectively；
Control system (14) also has timing module, passes through operation control system (14) customization temperature pattern and rotation in advance
The working time of pedestal (2).
2. strainer antianaphylaxis detection device according to claim 1, which is characterized in that the sealing temperature control compartment A (13)
It is inside equipped with the first temperature sensor (12), the first temperature sensor (12) and the control system (14) electrical connection；Seal temperature control
Second temperature sensor (8) are equipped in storehouse B (7), second temperature sensor (8) and the control system (14) electrical connection；Control
System (14) has temperature display panel.
3. strainer antianaphylaxis detection device according to claim 2, which is characterized in that partition has the first airflow channel
(4) and the second airflow channel (5), sealing temperature control compartment A (13) pass through the workbench (1) of the first airflow channel (4) and partition side
Upper space connection, sealing temperature control compartment B (7) pass through workbench (1) upper space of the second airflow channel (5) and the partition other side
Connection.
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| CN201710120809.1A CN106771116B (en) | 2017-03-02 | 2017-03-02 | A kind of strainer antianaphylaxis detection method and device |
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| CN201710120809.1A CN106771116B (en) | 2017-03-02 | 2017-03-02 | A kind of strainer antianaphylaxis detection method and device |
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| CN106771116B true CN106771116B (en) | 2018-11-30 |
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| CN107764987A (en) * | 2017-09-05 | 2018-03-06 | 广州市微生物研究所 | A kind of air purifier removes the detection method of house dust mite antigen effect |
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| CN102671488A (en) * | 2011-03-12 | 2012-09-19 | 东丽纤维研究所（中国）有限公司 | Air filter and application thereof |
| CN104083758A (en) * | 2014-07-28 | 2014-10-08 | 北京新华联协和药业有限责任公司 | Platanus pollen allergen mixture |
| CN206497122U (en) * | 2017-03-02 | 2017-09-15 | 中国家用电器研究院 | A kind of filter screen antianaphylaxis detection means |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE521370T1 (en) * | 2005-07-12 | 2011-09-15 | Stepan Co | COMPOSITION AND METHOD FOR DEACTIVATION OF ALLERGENIC PROTEINS ON SURFACES |
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2017
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| CN102671488A (en) * | 2011-03-12 | 2012-09-19 | 东丽纤维研究所（中国）有限公司 | Air filter and application thereof |
| CN104083758A (en) * | 2014-07-28 | 2014-10-08 | 北京新华联协和药业有限责任公司 | Platanus pollen allergen mixture |
| CN206497122U (en) * | 2017-03-02 | 2017-09-15 | 中国家用电器研究院 | A kind of filter screen antianaphylaxis detection means |
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