CN102586108A - Biological safety constant-temperature vibratory cultivating box - Google Patents

Biological safety constant-temperature vibratory cultivating box Download PDF

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Publication number
CN102586108A
CN102586108A CN2012100638282A CN201210063828A CN102586108A CN 102586108 A CN102586108 A CN 102586108A CN 2012100638282 A CN2012100638282 A CN 2012100638282A CN 201210063828 A CN201210063828 A CN 201210063828A CN 102586108 A CN102586108 A CN 102586108A
Authority
CN
China
Prior art keywords
operational zone
crosswind road
temperature
air
casing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2012100638282A
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Chinese (zh)
Inventor
谢清华
徐永光
甘宜梧
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JINAN BIOBASE BIOTECH CO Ltd
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JINAN BIOBASE BIOTECH CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by JINAN BIOBASE BIOTECH CO Ltd filed Critical JINAN BIOBASE BIOTECH CO Ltd
Priority to CN2012100638282A priority Critical patent/CN102586108A/en
Publication of CN102586108A publication Critical patent/CN102586108A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14Incubators; Climatic chambers

Abstract

The invention discloses a biological safety constant-temperature vibratory cultivating box. The biological safety constant-temperature vibratory cultivating box comprises a box body, a vibrating device, a vibrating power system and an air conditioning device, wherein the vibrating device is arranged in the box body; a door is arranged on the box body; a fan and a filter are arranged in the box body; the interior of the box body is partitioned into an operation area, a bottom air duct and a side air duct by partition boards; the bottom air duct is positioned below the operation area; the side air duct is positioned at one side part of the operation area; the upper part of the side air duct is communicated with the operation area; the lower part of the side air duct is communicated with the bottom air duct; and the bottom air duct is communicated with the operation area. According to the invention, the gas in the box body is enabled to flow circularly through the fan, and aerosol in the gas is filtered by the filter, so that the aerosol in the box body can be effectively eliminated. The biological safety constant-temperature vibratory cultivating box disclosed by the invention can prevent cross infection of test specimen, and is an ideal instrument for cell and microorganism culture as well as biological and chemical specimen tests. Operators can be protected and the environment pollution risk can be reduced.

Description

Biosafety constant-temperature shaking culture case
Technical field
The present invention relates to the constant-temperature shaking culture case of a kind of culturing micro-organisms and cell.
Background technology
The constant-temperature shaking culture case is widely used in the BOD that the water body that temperature and vibration have higher requirements is analyzed is measured, and the cultivation of bacterium, mikrobe etc. is preserved, plant growing, occasions such as breeding experiment.In environment protection, scientific research, production departments such as medical educational, health and epidemic prevention, medicine inspection, zoology and botany, ocean science food engineering have extensively and important use.
Existing dynamic system of constant-temperature shaking culture case and oscillatory system provide rotation/to-and-fro movement, keep higher the temperature inside the box precision through airflow circulation, thereby reach the required envrionment conditions of experiment.But existing constant-temperature shaking culture case can not effectively be handled the aerosol that incubator inside produces in carrying out processes such as cell, microorganism culturing, and the crossed contamination that the internal specimen that aerosol brings is arranged, thus the final precision and the result of influence experiment.Simultaneously experiment beginning, switch door and door design itself and the use of long period when finishing produce poorly sealed, as easy as rolling off a logly cause aerocolloidal leakage in the case, experimenter, environment are brought huge potential safety hazard.
Summary of the invention
The problem that the present invention will solve is: the present invention provides a kind of can effectively eliminate aerocolloidal Biosafety constant-temperature shaking culture case in the case.
In order to solve the problems of the technologies described above; The present invention includes and be provided with oscillation device, vibration power system and air-conditioning plant in casing, the casing, casing is provided with door, is provided with blower fan and strainer in the described casing; Be separated into the operational zone, be positioned at the air channel, the end of below, operational zone and be positioned at the crosswind road of operational zone sidepiece through dividing plate in the casing; The top in crosswind road communicates with the operational zone, and the bottom in crosswind road communicates with air channel, the end, and air channel, the end communicates with the operational zone.
For at the square one-tenth air curtain of the student; With gas and environmental gas in the isolated operational zone; Described door is arranged on the position that is positioned at the operational zone upside on the casing, and the crosswind road is communicated with through even air regulator with the operational zone, the lower end of sparing air regulator with respect to the upper end of sparing air regulator to crosswind road inner inclination.
For easy to make; Dividing plate between described operational zone and the crosswind road and even air regulator are integral structure; There is the bottom to extend into the V-shaped groove of the horizontal expansion in the crosswind road on the dividing plate between operational zone and the crosswind road; Be distributed with a plurality of air-permeating holes on the upper side wall of V-shaped groove, the upper side wall of V-shaped groove is described even air regulator.Directly on dividing plate, squeeze out V-shaped groove, the processing ventilation hole forms even air regulator on the V-shaped groove upper wall, need not make even air regulator separately, does not need web member to be connected between even air regulator and the dividing plate, makes simply, and cost is low.
In order to improve filter effect, described strainer is a high efficiency filter.
For not overslaugh operation, described crosswind road is positioned at the rear side of operational zone.
In order to improve filter effect, described blower fan is arranged on the position of air channel, the end and the connection of crosswind road, and described air-conditioning plant and strainer are arranged in the crosswind road.
Arrange that for ease the transmission mechanism of described vibration power system, connection oscillation device and vibration power system is arranged in air channel, the end, the pallet of described oscillation device is arranged in the operational zone.
For the ease of temperature in the control casing; Be provided with TP in the described casing; Described air-conditioning plant is connected with unit; Unit is used for the temperature of the detection of interior temperature of preset casing and displays temperature transmitter, C.T. sensor detecting temperature and design temperature, and according to comparative result control air-conditioning plant.
Beneficial effect of the present invention: the present invention flows the intravital gas circulation of case through blower fan, filters out the aerosol in the gas through strainer, thereby can effectively eliminate the intravital aerosol of case.Can prevent the cross infection of test sample, be cell, microorganism culturing, the desirable instrument biological, that chemical sample is tested.Can protect operator, reduce the environmental pollution risk.
Description of drawings
Fig. 1 is an internal structure synoptic diagram of the present invention;
Among the figure: 1, vibration power system, 2, oscillation device, 2-1, pallet, 3, air channel, the end, 4, blower fan; 5, high efficiency filter, 6, air-conditioning plant, 7, casing, 8, radiator fan, 9, the crosswind road; 10, door hinge, 11, even air regulator, 12, the loam cake door, 13, the operational zone.
Embodiment
A kind of specific embodiment as shown in Figure 1, it comprises and is provided with oscillation device 2, vibration power system 1, air-conditioning plant 6, blower fan 4 and high efficiency filter 5 in casing 7, the casing 7.
Casing 7 is provided with loam cake door 12; Be separated into operational zone 13, be positioned at the air channel, the end 3 of 13 belows, operational zone and be positioned at the crosswind road 9 of operational zone 13 rear sides through dividing plate in the casing 7; The top in crosswind road 9 communicates with operational zone 13; The bottom in crosswind road 9 communicates with air channel, the end 3, and air channel, the end 3 communicates with operational zone 13.Loam cake door 12 is positioned at the upside of operational zone 13, and loam cake door 12 is connected through door hinge 10 with casing 7.
Crosswind road 9 is communicated with through even air regulator 11 with operational zone 13, and even air regulator 11 is ventilative window, with the top plate body that is furnished with a plurality of air-permeating holes.Even air regulator 11 is obliquely installed, the lower end of even air regulator 11 with respect to the upper end of even air regulator to crosswind road 9 inner inclination.
Dividing plate between operational zone 13 and the crosswind road 9 and even air regulator 11 are integral structure; There is the bottom to extend into the V-shaped groove of the horizontal expansion in the crosswind road on the dividing plate between operational zone 13 and the crosswind road 9; Be distributed with a plurality of air-permeating holes on the upper side wall of V-shaped groove, the upper side wall of V-shaped groove is described even air regulator 11.
Blower fan 4 is arranged on the position of air channel, the end 3 and 9 connections of crosswind road, and air-conditioning plant 6 is arranged in the crosswind road 9 with high efficiency filter 5.Outside casing 7, also be provided with the radiator fan 8 that connects air-conditioning plant.The transmission mechanism of vibration power system 1, connection oscillation device 2 and vibration power system 1 is arranged in air channel, the end 3, and the pallet 2-1 of oscillation device 2 is arranged in operational zone 13.Oscillation device 2 all adopts prior art with vibration power system 1.
Be provided with TP in the casing 7; Described air-conditioning plant 6 is connected with unit; Unit is used for the temperature of the detection of preset casing 7 interior temperature and displays temperature transmitter, C.T. sensor detecting temperature and design temperature, and according to comparative result control air-conditioning plant.
Principle of work: required drive when vibration power system 1 provides product work, through oscillation device 2 conversions, realize the pallet 2-1 rotation and the to-and-fro movement of oscillation device 2.Circulating inside air-flow through blower fan 4 generations; Forming air channel, the end 3, crosswind road 9 and operational zone 13 interior gases effectively circulates; The refrigerating duty (perhaps heat) that air-conditioning plant in the crosswind road 96 is produced in time is sent in the operational zone 13, guarantees the precision and the homogeneity of temperature in the operational zone 13.The aerosol that in the airflow circulation process, test sample in the operational zone 13 is produced in time takes crosswind road 9 to, has high efficiency filter 5 to filter the back and removes.To filter the back clean gas simultaneously and take in the operational zone 13, prevent the cross infection of sample.Simultaneously, because the special construction of even air regulator, during 9 entering operational zones 13, the oblique operational zone that flows into down of air-flow forms air curtain, gas and environmental gas in the effectively isolated operational zone 13 to air-flow below loam cake door 12 from the crosswind road.
High efficiency filter is mainly used in particle dust and the various suspended substance that captures below the 0.5um.Adopt beta glass fibre paper to make filtrate, be widely used in biomedicine field at present.

Claims (8)

1. Biosafety constant-temperature shaking culture case; Comprise being provided with oscillation device, vibration power system and air-conditioning plant in casing, the casing, casing is provided with door, it is characterized in that: be provided with blower fan and strainer in the described casing; Be separated into the operational zone, be positioned at the air channel, the end of below, operational zone and be positioned at the crosswind road of operational zone sidepiece through dividing plate in the casing; The top in crosswind road communicates with the operational zone, and the bottom in crosswind road communicates with air channel, the end, and air channel, the end communicates with the operational zone.
2. Biosafety constant-temperature shaking culture case according to claim 1; It is characterized in that: described door is arranged on the position that is positioned at the operational zone upside on the casing; Crosswind road and operational zone are communicated with through even air regulator, the lower end of even air regulator with respect to the upper end of sparing air regulator to crosswind road inner inclination.
3. Biosafety constant-temperature shaking culture case according to claim 2; It is characterized in that: dividing plate between described operational zone and the crosswind road and even air regulator are integral structure; There is the bottom to extend into the V-shaped groove of the horizontal expansion in the crosswind road on the dividing plate between operational zone and the crosswind road; Be distributed with a plurality of air-permeating holes on the upper side wall of V-shaped groove, the upper side wall of V-shaped groove is described even air regulator.
4. Biosafety constant-temperature shaking culture case according to claim 1 is characterized in that: described strainer is a high efficiency filter.
5. Biosafety constant-temperature shaking culture case according to claim 1 is characterized in that: described crosswind road is positioned at the rear side of operational zone.
6. according to any one described Biosafety constant-temperature shaking culture case among the claim 1-5, it is characterized in that: described blower fan is arranged on the position of air channel, the end and the connection of crosswind road, and described air-conditioning plant and strainer are arranged in the crosswind road.
7. Biosafety constant-temperature shaking culture case according to claim 6 is characterized in that: the transmission mechanism of described vibration power system, connection oscillation device and vibration power system is arranged in air channel, the end, and the pallet of described oscillation device is arranged in the operational zone.
8. Biosafety constant-temperature shaking culture case according to claim 6; It is characterized in that: be provided with TP in the described casing; Described air-conditioning plant is connected with unit; Unit is used for the temperature of the detection of interior temperature of preset casing and displays temperature transmitter, C.T. sensor detecting temperature and design temperature, and according to comparative result control air-conditioning plant.
CN2012100638282A 2012-03-13 2012-03-13 Biological safety constant-temperature vibratory cultivating box Pending CN102586108A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2012100638282A CN102586108A (en) 2012-03-13 2012-03-13 Biological safety constant-temperature vibratory cultivating box

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2012100638282A CN102586108A (en) 2012-03-13 2012-03-13 Biological safety constant-temperature vibratory cultivating box

Publications (1)

Publication Number Publication Date
CN102586108A true CN102586108A (en) 2012-07-18

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103923163A (en) * 2013-01-15 2014-07-16 北京金麦格生物技术有限公司 Workbench system and the method for bioactive substance extractor
CN103923830A (en) * 2013-01-15 2014-07-16 北京金麦格生物技术有限公司 Microenvironment improvement method and system for bioactive substance extractor
CN104877891A (en) * 2015-05-22 2015-09-02 邢孝玲 Intravenous injection drug cultivating safety cabinet
CN105532314A (en) * 2016-01-11 2016-05-04 中国农业科学院作物科学研究所 Low-concentration carbon dioxide incubator for basic scientific research and control method
CN106399050A (en) * 2016-11-17 2017-02-15 成都聚智工业设计有限公司 Culture box for microorganism
CN106554918A (en) * 2016-05-12 2017-04-05 厦门医学高等专科学校 A kind of microorganism cultivates integration apparatus
CN108587908A (en) * 2017-12-27 2018-09-28 上海力申科学仪器有限公司 It is uniformly distributed with gas and the use for laboratory incubator of high efficiency filter
CN113881566A (en) * 2021-11-01 2022-01-04 孙蕾蕾 Intelligent efficient variable-temperature embryo incubator based on Internet and use method
EP4293101A1 (en) * 2022-06-14 2023-12-20 Asklepios Biopharmaceutical, Inc. Reactor with temperature control and method of using the same

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2477646Y (en) * 2001-05-08 2002-02-20 冯超 Biochemical cultivation case
CN2677384Y (en) * 2004-02-19 2005-02-09 刘怀增 Drive of microbiological thermostatic vibration culturing box
CN201045140Y (en) * 2006-12-27 2008-04-09 甘宜梧 Organism safe incubator
CN201940232U (en) * 2010-12-03 2011-08-24 上海力申科学仪器有限公司 Multipurpose biosafety cabinet capable of high-speed circulation
CN202460648U (en) * 2012-03-13 2012-10-03 济南鑫贝西生物技术有限公司 Biological safety thermostatic shaking incubator

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2477646Y (en) * 2001-05-08 2002-02-20 冯超 Biochemical cultivation case
CN2677384Y (en) * 2004-02-19 2005-02-09 刘怀增 Drive of microbiological thermostatic vibration culturing box
CN201045140Y (en) * 2006-12-27 2008-04-09 甘宜梧 Organism safe incubator
CN201940232U (en) * 2010-12-03 2011-08-24 上海力申科学仪器有限公司 Multipurpose biosafety cabinet capable of high-speed circulation
CN202460648U (en) * 2012-03-13 2012-10-03 济南鑫贝西生物技术有限公司 Biological safety thermostatic shaking incubator

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103923163A (en) * 2013-01-15 2014-07-16 北京金麦格生物技术有限公司 Workbench system and the method for bioactive substance extractor
CN103923830A (en) * 2013-01-15 2014-07-16 北京金麦格生物技术有限公司 Microenvironment improvement method and system for bioactive substance extractor
CN103923830B (en) * 2013-01-15 2016-06-29 常州金麦格生物技术有限公司 The microenvironment improvements method and system of bioactive substance extraction apparatus
CN103923163B (en) * 2013-01-15 2017-05-24 常州金麦格生物技术有限公司 Workbench system and the method for bioactive substance extractor
CN104877891A (en) * 2015-05-22 2015-09-02 邢孝玲 Intravenous injection drug cultivating safety cabinet
CN105532314A (en) * 2016-01-11 2016-05-04 中国农业科学院作物科学研究所 Low-concentration carbon dioxide incubator for basic scientific research and control method
CN106554918A (en) * 2016-05-12 2017-04-05 厦门医学高等专科学校 A kind of microorganism cultivates integration apparatus
CN106554918B (en) * 2016-05-12 2019-04-16 厦门医学院 A kind of microculture integration apparatus
CN106399050A (en) * 2016-11-17 2017-02-15 成都聚智工业设计有限公司 Culture box for microorganism
CN108587908A (en) * 2017-12-27 2018-09-28 上海力申科学仪器有限公司 It is uniformly distributed with gas and the use for laboratory incubator of high efficiency filter
CN113881566A (en) * 2021-11-01 2022-01-04 孙蕾蕾 Intelligent efficient variable-temperature embryo incubator based on Internet and use method
EP4293101A1 (en) * 2022-06-14 2023-12-20 Asklepios Biopharmaceutical, Inc. Reactor with temperature control and method of using the same

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Application publication date: 20120718