CN210030662U

CN210030662U - Inserting piece device for culturing actinomycetes

Info

Publication number: CN210030662U
Application number: CN201920597184.2U
Authority: CN
Inventors: 熊泽; 邵伟
Original assignee: China Three Gorges University CTGU
Current assignee: China Three Gorges University CTGU
Priority date: 2019-04-28
Filing date: 2019-04-28
Publication date: 2020-02-07
Anticipated expiration: 2029-04-28

Abstract

The utility model discloses an inserted sheet device for actinomycete is cultivateed, including glass lid, go up inserted sheet board, end box down, glass lid covers on last inserted sheet board disc, goes up the inserted sheet board and inserts on the inserted sheet double-layered post of inserted sheet board down through the inserted sheet groove cover, and lower inserted sheet board is installed in end box, and the second screw is fixed in on the inserted sheet board with end box, lower inserted sheet board through the fixed ear in end box both sides, second screw hole and last inserted sheet board first screw hole. The device has solved the problem that the actinomycete inserted sheet was cultivateed betterly, has made things convenient for the inserted sheet that the actinomycete was cultivateed to realize quick, efficient inserted sheet, reduced the damage of inserted sheet, improved the experimental effect.

Description

Inserting piece device for culturing actinomycetes

Technical Field

The utility model relates to an inserted sheet device, especially an inserted sheet device is used in actinomycete cultivation belongs to microbial cultivation film-making technical field.

Background

The observation of the actinomycete hypha is an inserting method, namely, the actinomycete hypha is streaked and inoculated on a culture medium, and a sterilized cover glass is inserted for culture, so that the actinomycete hypha grows along the intersection of the culture medium and the cover glass and is attached to the cover glass. During observation, the cover glass is taken out slightly and placed on a glass slide for direct microscopic examination. The method maintains the natural growth state of the actinomycetes and is convenient for observing the growth characteristics of the actinomycetes in the natural growth state. However, in the process of inserting the pieces, because the pieces are needed to be inserted one by one, the inserting efficiency is very low, meanwhile, the exposure time of the culture medium is long, the contamination of the mixed bacteria is easily caused, and the cover glass is easily damaged in the process of picking by tweezers, so that the observation of hyphae is influenced.

Disclosure of Invention

The utility model provides an inserted sheet device for actinomycete is cultivateed, the device can solve the problem that the actinomycete inserted sheet was cultivateed betterly, cover glass's inserted sheet when making things convenient for the actinomycete to can realize quick, efficient inserted sheet, reduce the damage of inserted sheet, improve the experiment effect.

The utility model adopts the technical proposal that: an inserting piece device for culturing actinomycetes comprises a bottom box, wherein a lower inserting piece plate is arranged in the bottom box, an upper inserting piece plate is connected onto the bottom box, a groove is formed in the side wall of the lower inserting piece plate, a spring is arranged in the groove, a button is arranged on the other side of the lower inserting piece plate opposite to the groove, the spring and the button are located on the same axis, a notch is formed in the side wall of the bottom box, and the outer side of the button penetrates through the notch to be communicated with the outside; a plurality of inserting sheet clamping columns are arranged on the lower inserting sheet plate, a plurality of inserting sheet holes corresponding to the inserting sheet clamping columns are communicated on the upper inserting sheet plate, and gaps exist after the inserting sheet holes and the inserting sheet clamping columns are sleeved.

Furthermore, two sliding grooves are formed in the lower inserting sheet plate and are parallel to the axis of the spring and the axis of the button; be equipped with two guide posts in every spout, the top of guide post is equipped with the fourth screw hole, goes up inserted sheet board top and is equipped with corresponding third screw hole, and first bolt passes third screw hole and fourth screw hole and guide post is fixed.

Further, still be equipped with two fixed ears on the lateral wall of end box, be equipped with the second screw hole on the fixed ear, two fixed ears are located the both sides of spring and button place axis respectively, go up inserted sheet lateral wall and are equipped with corresponding first screw hole, and the second bolt passes second screw hole and first screw hole and will end box and go up the inserted sheet board fixedly.

Furthermore, a glass cover is further arranged on the upper insertion plate, and the glass cover is a dish cover which is hollow and one end of which is sealed.

Furthermore, the insert clamping column is a horizontal straight parallelepiped, the side edge and the spring of the insert clamping column are perpendicular to the axis where the button is located, the shape and the size of the insert hole are matched with the insert clamping column, and the gap formed after the insert hole and the insert clamping column are sleeved is 5-6 times of the thickness of a cover glass to be inserted.

Furthermore, in the horizontal straight parallelepiped inserting sheet clamping column, two end faces are parallelograms, the upper bottom face and the lower bottom face are rectangles, two side faces are also rectangles, the included angle between the two side faces and the plane where the lower inserting sheet plate is located is 60 degrees, and the expansion amount of the spring is larger than the gap existing after the inserting sheet hole and the inserting sheet clamping column are sleeved.

Furthermore, the upper inserting sheet plate comprises a disc at the upper part and an oval bottom plate connected with the disc; the lower inserting sheet plate is an oval flat plate, the spring and the button are positioned on the long axis of the lower inserting sheet plate, the bottom box is of an oval box-shaped structure, the long axis of the oval bottom plate is equal to the long axis of the edge of the inner wall of the bottom box, and the short axis of the oval bottom plate is equal to the short axis of the edge of the inner wall of the bottom box; the long axis of the lower insertion plate is smaller than the long axis of the edge of the inner wall of the bottom box, and the short axis of the lower insertion plate is equal to the short axis of the edge of the inner wall of the bottom box; the diameter of the disc is equal to the minor axis of the elliptical bottom plate; the height of the insert clamping column is 2-3mm, the depth of the insert hole is 2-3mm, the width of the insert clamping column is 20-22mm, and the width of the insert hole is 21-23 mm.

The utility model discloses following beneficial effect has:

1. through adopting end box, the built-in lower insert plate that establishes of end box, connect the insert plate on the end box, the cover glass that the grafting was treated in the centre gripping between insert plate and the last insert plate down can realize the quick dress of polylith cover glass and insert, and the insert is efficient, need not use tweezers moreover, avoids the emergence of the broken condition of cover glass.

2. The lower sheet inserting plate is provided with an sheet inserting clamping column, the upper sheet inserting plate is provided with a sheet inserting hole matched with the sheet inserting clamping column, and a gap is formed after the sheet inserting hole and the sheet inserting clamping column are sleeved, so that the slide to be inserted can be conveniently installed and clamped; in addition, a groove is formed in the lower inserting sheet plate, a spring is arranged in the groove, a button is arranged on the other side of the lower inserting sheet plate opposite to the groove, the lower inserting sheet plate can extrude the spring by pushing the button, relative sliding is generated between the lower inserting sheet plate and the upper inserting sheet plate, and the cover glass is convenient to mount and release.

3. The use of the upper sliding groove of the lower insert plate, the guide column and the first nut can enable the moving direction between the lower insert plate and the upper insert plate to be more accurate, and avoid the deviation. An included angle exists between the insert clamping column and the lower insert plate, so that a cover glass which is inserted into the culture medium in a later period has a certain included angle, and the growth of actinomycete hyphae is facilitated.

4. The use of the glass cover facilitates the sterilization treatment.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

in the figure: glass lid 1, first screw 2, go up inserted sheet board 3, guide post 4, spring 5, inserted sheet board 6 down, end box 7, second screw 8, button 9, oval bottom plate 301, disc 302, third screw hole 303, inserted sheet hole 304, first screw hole 305, fourth screw hole 401, inserted sheet presss from both sides post 601, spout 602, recess 603, fixed ear 701, second screw hole 702, notch 703.

Detailed Description

The invention is further described with reference to the following examples, but the scope of the invention as claimed is not limited to the scope of the examples.

As shown in figure 1, the inserting piece device for culturing actinomycetes comprises a bottom box 7, wherein a lower inserting piece plate 6 is arranged in the bottom box 7, an upper inserting piece plate 3 is connected onto the bottom box 7, a groove 603 is formed in the side wall of the lower inserting piece plate 6, a spring 5 is arranged in the groove 603, a button 9 is arranged on the other side of the lower inserting piece plate 6 opposite to the groove 603, the spring 5 and the button 9 are positioned on the same axis, a notch 703 is formed in the side wall of the bottom box 7, and the outer side of the button 9 penetrates through the notch 703 to be communicated with the outside; a plurality of inserting sheet clamping columns 601 are arranged on the lower inserting sheet plate 6, a plurality of inserting sheet holes 304 corresponding to the inserting sheet clamping columns 601 penetrate through the upper inserting sheet plate 3, and gaps exist after the inserting sheet holes 304 are sleeved with the inserting sheet clamping columns 601.

In a preferable scheme, two sliding grooves 602 are formed in the lower insert plate 6, and the sliding grooves 602 are parallel to the axis where the spring 5 and the button 9 are located; be equipped with two guide posts 4 in every spout 602, the top of guide post is equipped with fourth screw hole 401, goes up inserted sheet board 3 top and is equipped with corresponding third screw hole 303, and first bolt 2 passes third screw hole 303 and fourth screw hole 401 and guide post is fixed. Through the setting, when pressing button 9, lower inserted sheet board 6 removes along the axis at spring 5 and button 9 place, avoids the skew and the later stage can't press from both sides tightly the inserted sheet.

In a preferable scheme, two fixing lugs 701 are further arranged on the side wall of the bottom box 7, second threaded holes 702 are formed in the fixing lugs 701, the two fixing lugs 701 are respectively located on two sides of the axis where the spring 5 and the button 9 are located, corresponding first threaded holes 305 are formed in the side wall of the upper inserting plate 3, and the second bolt 8 penetrates through the second threaded holes 702 and the first threaded holes 305 to fix the bottom box 7 and the upper inserting plate 3. Through going up inserted sheet board 3 and end box 7 fixed, avoid removing the in-process of inserting the piece box down and lead to the fact the influence to going up inserted sheet board 3.

In the preferred scheme, the upper inserting sheet plate 3 is further provided with a glass cover 1 which is a hollow dish cover with one closed end. The adoption of the dish cover is convenient for disinfection, and the upper inserting sheet plate 3 after disinfection is prevented from being polluted as much as possible.

In the preferred scheme, the insert clamping column 601 is a horizontal straight parallelepiped, the side edge of the insert clamping column is vertical to the axis where the spring 5 is located, the shape and the size of the insert hole 304 are matched with the insert clamping column, and the gap existing after the insert hole 304 is sleeved with the insert clamping column 601 is 5-6 times of the thickness of a cover glass to be inserted.

Furthermore, in the horizontal straight parallelepiped insert clamping column 601, two end faces are parallelograms, an upper bottom face and a lower bottom face are rectangles, two side faces are also rectangles, an included angle between the two side faces and a plane where the lower insert plate 6 is located is 60 degrees, and the expansion amount of the spring 5 is larger than that of a gap existing after the insert hole 304 and the insert clamping column 601 are sleeved.

Furthermore, the upper insert plate 3 comprises a disk 302 at the upper part and an oval bottom plate 301 connected with the disk 302; the lower insert plate 6 is an oval flat plate, the spring 5 and the button 9 are positioned on the long axis of the lower insert plate 6, the bottom box 7 is an oval box-shaped structure, the long axis of the oval bottom plate 301 is equal to the long axis of the edge of the inner wall of the bottom box 7, and the short axis of the oval bottom plate 301 is equal to the short axis of the edge of the inner wall of the bottom box 7; the long axis of the lower insert plate 6 is smaller than the long axis of the edge of the inner wall of the bottom box 7, and the short axis of the lower insert plate 6 is equal to the short axis of the edge of the inner wall of the bottom box 7; the diameter of the disk 302 is equal to the minor axis of the elliptical base 301; the height of the inserting sheet clamping column 601 is 2-3mm, the depth of the inserting sheet hole 304 is 2-3mm, the width of the inserting sheet clamping column 601 is 20-22mm, and the width of the inserting sheet hole 304 is 21-23 mm.

The device is used for the following specific operations:

1) uncovering the glass cover 1 of this device, pressing button 9 on one hand, making lower inserted sheet board 6 extrude the spring, slightly to the spring orientation certain, form the gap between inserted sheet hole 304 and inserted sheet holder 601, another hand will cover the glass slide (if: 18 multiplied by 18 mm) are sequentially plugged into each gap, each gap is filled with one piece until the gap is full, the button 9 is stopped to be pressed, the lower inserting piece plate rebounds towards the direction of the button, and the inserting piece clamping column is matched with the inserting piece hole to tightly squeeze the cover glass;

2) covering the glass cover 1, and performing sterilization operation on the device;

3) cooling to room temperature for later use after sterilization;

4) marking the actinomycete strain on an actinomycete culture plate;

5) uncovering the glass cover 1 of the device, turning the device for 180 degrees, aligning the device with an actinomycete culture plate, slowly pressing down the device on an actinomycete culture medium, simultaneously pressing a button 9 to release a cover glass, and then lifting the device to complete the operation of inserting the sheet;

6) and (4) covering a dish cover of the actinomycete culture plate, and transferring the culture medium into an incubator for culture.

Claims

1. The utility model provides an actinomycete is cultivateed and is used inserted sheet device which characterized in that: the novel film packaging box comprises a bottom box (7), wherein a lower insert plate (6) is arranged in the bottom box (7), an upper insert plate (3) is connected onto the bottom box (7), a groove (603) is formed in the side wall of the lower insert plate (6), a spring (5) is arranged in the groove (603), a button (9) is arranged on the other side of the lower insert plate (6) opposite to the groove (603), the spring (5) and the button (9) are located on the same axis, a notch (703) is formed in the side wall of the bottom box (7), and the outer side of the button (9) penetrates through the notch (703) to be communicated with the outside; a plurality of inserting sheet clamping columns (601) are arranged on the lower inserting sheet plate (6), a plurality of inserting sheet holes (304) corresponding to the inserting sheet clamping columns (601) penetrate through the upper inserting sheet plate (3), and gaps exist after the inserting sheet holes (304) are sleeved with the inserting sheet clamping columns (601).

2. The apparatus of claim 1, wherein: two sliding grooves (602) are formed in the lower insertion plate (6), and the sliding grooves (602) are parallel to the axis where the spring (5) and the button (9) are located; be equipped with two guide posts (4) in every spout (602), the top of guide post is equipped with fourth screw hole (401), goes up inserted sheet board (3) top and is equipped with corresponding third screw hole (303), and first bolt (2) pass third screw hole (303) and fourth screw hole (401) and are fixed with the guide post.

3. The apparatus of claim 1, wherein: still be equipped with two fixed ears (701) on the lateral wall of end box (7), be equipped with second screw hole (702) on fixed ear (701), two fixed ears (701) are located the both sides of spring (5) and button (9) place axis respectively, go up insertion piece board (3) lateral wall and are equipped with corresponding first screw hole (305), second bolt (8) pass second screw hole (702) and first screw hole (305) with end box (7) and last insertion piece board (3) fixed.

4. The apparatus of claim 1, wherein: the upper inserting plate (3) is also provided with a glass cover (1) which is a hollow dish cover with one closed end.

5. The apparatus of any one of claims 1-4, wherein: the insert clamping column (601) is a horizontal straight parallelepiped, the side edge of the insert clamping column is perpendicular to the axis where the spring (5) and the button (9) are located, the shape and the size of the insert hole (304) are matched with the insert clamping column, and a gap existing after the insert hole (304) is sleeved with the insert clamping column (601) is 5-6 times of the thickness of a cover glass to be inserted.

6. The apparatus of claim 5, wherein: in horizontal straight parallelepiped inserted sheet press from both sides post (601), the both ends face is parallelogram, goes up the bottom surface and is the rectangle with lower bottom surface, and both sides face also is the rectangle, and the planar contained angle in both sides face and lower inserted sheet board (6) place is 60 degrees, the flexible volume of spring (5) is greater than and has the gap after inserted sheet hole (304) and inserted sheet press from both sides post (601) suit.

7. The apparatus of claim 5, wherein: the upper inserting plate (3) comprises a disc (302) at the upper part and an oval bottom plate (301) connected with the disc (302); the lower insertion plate (6) is an oval flat plate, the spring (5) and the button (9) are positioned on the long axis of the lower insertion plate (6), the bottom box (7) is of an oval box-shaped structure, the long axis of the oval bottom plate (301) is equal to the long axis of the edge of the inner wall of the bottom box (7), and the short axis of the oval bottom plate (301) is equal to the short axis of the edge of the inner wall of the bottom box (7); the long axis of the lower insertion plate (6) is smaller than the long axis of the edge of the inner wall of the bottom box (7), and the short axis of the lower insertion plate (6) is equal to the short axis of the edge of the inner wall of the bottom box (7); the diameter of the disc (302) is equal to the minor axis of the elliptical base plate (301).

8. The apparatus of claim 7, wherein: the height of the inserting sheet clamping column (601) is 2-3mm, the depth of the inserting sheet hole (304) is 2-3mm, the width of the inserting sheet clamping column (601) is 20-22mm, and the width of the inserting sheet hole (304) is 21-23 mm.