CN216237093U - Double-chamber permselectivity cell culture device - Google Patents
Double-chamber permselectivity cell culture device Download PDFInfo
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- CN216237093U CN216237093U CN202123047931.1U CN202123047931U CN216237093U CN 216237093 U CN216237093 U CN 216237093U CN 202123047931 U CN202123047931 U CN 202123047931U CN 216237093 U CN216237093 U CN 216237093U
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- cell culture
- culture device
- dual chamber
- chamber
- permselective
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- 238000004113 cell culture Methods 0.000 title claims abstract description 33
- 239000010409 thin film Substances 0.000 claims description 10
- 239000002985 plastic film Substances 0.000 claims description 8
- 238000003860 storage Methods 0.000 claims description 5
- 230000009977 dual effect Effects 0.000 claims 6
- 230000005389 magnetism Effects 0.000 claims 1
- 239000004033 plastic Substances 0.000 claims 1
- 230000035699 permeability Effects 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 19
- 239000010408 film Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 4
- 210000004748 cultured cell Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000010367 cloning Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The utility model discloses a double-chamber selective permeability cell culture device, which comprises a culture device body, wherein a plurality of springs are fixedly arranged on two sides of the inner wall of the culture device body, one end of one part of the springs is fixedly provided with a first culture plate, one end of the other part of the springs is fixedly provided with a second culture plate, the front surfaces of the first culture plate and the second culture plate are movably hinged with box covers, one sides of the two box covers are respectively provided with a groove, and the insides of the two grooves are respectively clamped and connected with a rubber tube. Improves the quality of cell culture.
Description
Technical Field
The utility model relates to the technical field of cell culture devices, in particular to a double-chamber selective permeability cell culture device.
Background
The cell culture refers to a method for simulating in-vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the in-vivo environment to survive, grow, reproduce and maintain main structure and functions, the cell culture is also called cell cloning technology, the formal term in biology is cell culture technology, the cell culture is an essential process for the whole bioengineering technology and one of the biological cloning technologies, the cell culture is the large-scale cloning of cells, the cell culture technology can enable one cell to be cultured into simple single cells or minimally differentiated multiple cells in a large scale, and a double-chamber selective permeable cell culture device is required to be selected for culturing two different cells.
However, the existing cell culture device still has some defects:
when the culture device cultures cells, the progress of cell culture and the reproduction condition of the cells need to be continuously checked, but the existing culture device needs to open more areas to contact the cells, so that the contact time of the cells and the outside air is prolonged, the quality of cell culture is reduced, the checking area cannot be effectively adjusted, and the application range of the culture device is reduced.
SUMMERY OF THE UTILITY MODEL
The present invention aims to provide a dual-chamber permselective cell culture device to solve the problems of the prior art that the prior art culture device needs to open a large area to contact with cells, the contact time between the cells and the outside air is increased, and the viewing area cannot be effectively adjusted.
In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a two room permselectivity cell culture devices, includes the culture apparatus body, the equal fixed mounting in both sides of culture apparatus body inner wall has a plurality of springs, one of them part the one end fixed mounting of spring has first cultivation board, another part the one end fixed mounting of spring has the second to cultivate the board, the equal movable hinge in front of first cultivation board and second cultivation board has the case lid, two one side of case lid is all seted up flutedly, two the equal block in inside of recess is connected with the rubber tube, two the equal fixed mounting in one side of case lid has the case, two one side of case all alternates and is connected with first pivot.
As a preferable technical scheme of the utility model, one end parts of the two first rotating shafts are respectively wound with a film glove, and two sides of the bottom ends of the two storage boxes are respectively fixedly provided with a supporting plate.
As a preferable technical scheme of the utility model, second rotating shafts are respectively inserted and connected between the inner walls of two adjacent supporting plates, and blades are fixedly sleeved at one end parts of the two second rotating shafts.
As a preferable technical solution of the present invention, the two blades are disposed at corresponding positions, and the opening diameters of the two second rotating shafts are smaller than the opening diameters of the two blades.
As a preferred technical scheme of the utility model, the top ends of the two box covers are respectively provided with an auxiliary groove, and plastic sheets are placed in the two auxiliary grooves.
According to a preferable technical scheme of the utility model, N-pole magnets are arranged on one sides of the two plastic sheets, S-pole magnets are magnetically connected to one sides of the two N-pole magnets, and one sides of the two S-pole magnets are respectively contacted with one sides of the two box covers.
As a preferable technical scheme of the utility model, one side of each of the two N-pole magnets is rotatably connected with a magnifying lens, and the positions of the two magnifying lenses are corresponding to each other.
Compared with the prior art, the utility model has the beneficial effects that:
1. the utility model relates to a double-chamber permselectivity cell culture device, which is provided with a rubber tube, a first rotating shaft, a film glove, a supporting plate, a blade and a second rotating shaft, wherein a box cover is movably hinged on one side of the first culture plate and one side of the second culture plate, the length of the film glove wound on one end part of the first rotating shaft is longer, the film glove is downwards pulled and pulled to a specific length, and then the film glove is contacted with the blade and slowly pressed inwards, so that the film glove can be divided and can be divided into proper lengths, the rubber tube is made of rubber and has enough flexibility, the hand of a worker wearing the film glove extends into the rubber tube, cultured cells can be checked, the problem that the cells are greatly contacted with outside air is solved through the structure, and the quality during cell culture is improved.
2. According to the double-chamber selective permeability cell culture device, the plastic sheet, the S-pole magnet, the N-pole magnet and the magnifier are arranged, the S-pole magnet and the N-pole magnet can attract each other, and the N-pole magnet can move along with the S-pole magnet in the process of sliding the S-pole magnet due to the fact that magnetic fields are generated by respective currents, and are firmly adsorbed on the surface of the plastic sheet, the magnifier can amplify the condition of cells, the detection efficiency is improved, the position of the S-pole magnet can be effectively adjusted according to the position detected by the cells, and the double-chamber selective permeability cell culture device is more convenient and convenient to use.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is an enlarged view of the utility model at A in FIG. 1;
FIG. 3 is a partial bottom view of the present invention;
fig. 4 is a partial front view structure diagram of the present invention.
In the figure: 1. a culture device body; 2. a first culture plate; 3. a second culture plate; 4. a spring; 5. a box cover; 6. a rubber tube; 7. a storage box; 8. a first rotating shaft; 9. a thin film glove; 10. a support plate; 11. a second rotating shaft; 12. a blade; 13. an N-pole magnet; 14. a magnifying glass; 15. an S-pole magnet; 16. a plastic sheet.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a dual-chamber permselective cell culture device, comprising:
the first embodiment is as follows:
as shown in FIGS. 1-3, a double-chamber permselective cell culture device comprises a culture device body 1, wherein a plurality of springs 4 are fixedly mounted on both sides of the inner wall of the culture device body 1, a first culture plate 2 is fixedly mounted at one end of one part of the springs 4, a second culture plate 3 is fixedly mounted at one end of the other part of the springs 4, box covers 5 are movably hinged on the front surfaces of the first culture plate 2 and the second culture plate 3, one side of each of the two box covers 5 is provided with a groove, rubber tubes 6 are respectively connected in the two grooves in a clamping manner, storage boxes 7 are respectively fixedly mounted on one side of each of the two box covers 5, a first rotating shaft 8 is respectively and alternately connected on one side of each of the two storage boxes 7, a second rotating shaft 11 is respectively and alternately connected between the inner walls of two adjacent support plates 10, a blade 12 is fixedly sleeved on one end of each of the two second rotating shafts 11, and is pulled to a specific length, the film glove 9 is in contact with the blade 12 and is slowly pressed inwards, so that the film glove 9 can be divided into appropriate lengths, the rubber tube 6 is made of rubber and has enough flexibility, and the hand of a worker wearing the film glove 9 extends into the rubber tube 6 to check cultured cells.
Example two:
on the basis of the first embodiment, as shown in fig. 1 and 4, the two blades 12 are arranged at corresponding positions, the opening diameters of the two second rotating shafts 11 are smaller than those of the two blades 12, the top ends of the two box covers 5 are both provided with auxiliary grooves, plastic sheets 16 are placed in the two auxiliary grooves, one sides of the two N-pole magnets 13 are rotatably connected with magnifying lenses 14, and the positions of the two magnifying lenses 14 are corresponding.
The working principle is as follows: two kinds of cells are respectively placed in the first culture plate 2 and the second culture plate 3 for culture, when the condition of cell culture needs to be detected, the thin film glove 9 is pulled downwards, the thin film glove 9 is pulled to a proper length, the proper length can be used for controlling fingers at the moment, after the proper length of the thin film glove 9 is found, a worker inclines the thin film glove 9 towards one side, the back surface of the thin film glove 9 is contacted with the blade 12, the thin film glove 9 is slowly and continuously pressed inwards again, the blade 12 on the sharp surface can divide the thin film glove 9 into proper lengths, the rubber tube 6 is made of rubber, the rubber tube 6 has enough flexibility, the hand of the worker wearing the thin film glove 9 extends into the rubber tube 6, the rubber tube 6 can deform to a certain degree, and the problem that the cells are greatly contacted with the outside air is solved, quality when having improved cell culture, the box of the cultured cell that will take out moves the back of magnifying glass 14, magnifying glass 14 can enlarge the cell, the reproductive aspect and the progress of cultivateing of cell can be more clear seen, can inter attraction between S utmost point magnet 15 and the N utmost point magnet 13, the reason has a magnetic field for electric current production separately, consequently at the in-process of sliding S utmost point magnet 15, N utmost point magnet 13 also can follow the removal, and firmly adsorb on the surface of plastic sheet 16, can effectually adjust S utmost point magnet 15 'S position according to the position that the cell detected, culture apparatus' S application range has been improved.
In the description of the present invention, it is to be understood that the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings and are only for convenience in describing the present invention and simplifying the description, but are not intended to indicate or imply that the indicated devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.
In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly attached, detachably attached, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A dual-chamber permselective cell culture device comprising a culture device body (1), characterized in that: the equal fixed mounting in both sides of culture apparatus body (1) inner wall has a plurality of springs (4), partly wherein the one end fixed mounting of spring (4) has first cultivation board (2), another part the one end fixed mounting of spring (4) has second cultivation board (3), the equal movable hinge in front of first cultivation board (2) and second cultivation board (3) has case lid (5), two one side of case lid (5) is all seted up flutedly, two the equal block in inside of recess is connected with rubber tube (6), two the equal fixed mounting in one side of case lid (5) has case (7), one side of two case (7) all alternates and is connected with first pivot (8).
2. A dual chamber permselective cell culture device according to claim 1, characterized in that: two a thin film glove (9) is wound on one end of the first rotating shaft (8), and two supporting plates (10) are fixedly mounted on two sides of the bottom end of the storage box (7).
3. A dual chamber permselective cell culture device according to claim 2, characterized in that: two adjacent all interlude between the inner wall of backup pad (10) is connected with second pivot (11), two the equal fixed cover of a tip of second pivot (11) is equipped with blade (12).
4. A dual chamber permselective cell culture device according to claim 3, characterized in that: the two blades (12) are arranged at corresponding positions, and the opening diameters of the two second rotating shafts (11) are smaller than those of the two blades (12).
5. A dual chamber permselective cell culture device according to claim 1, characterized in that: auxiliary grooves are formed in the top ends of the two box covers (5), and plastic thin sheets (16) are placed in the two auxiliary grooves.
6. A dual chamber permselective cell culture device according to claim 5, characterized in that: two one side of plastic sheet (16) all is equipped with N utmost point magnet (13), two the equal magnetism in one side of N utmost point magnet (13) is connected with S utmost point magnet (15), two one side of S utmost point magnet (15) contacts with one side of two case lids (5) respectively.
7. A dual chamber permselective cell culture device according to claim 6, characterized in that: one side of each of the two N-pole magnets (13) is rotatably connected with a magnifying lens (14), and the positions of the two magnifying lenses (14) correspond to each other.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123047931.1U CN216237093U (en) | 2021-12-06 | 2021-12-06 | Double-chamber permselectivity cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123047931.1U CN216237093U (en) | 2021-12-06 | 2021-12-06 | Double-chamber permselectivity cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216237093U true CN216237093U (en) | 2022-04-08 |
Family
ID=80961950
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123047931.1U Expired - Fee Related CN216237093U (en) | 2021-12-06 | 2021-12-06 | Double-chamber permselectivity cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216237093U (en) |
-
2021
- 2021-12-06 CN CN202123047931.1U patent/CN216237093U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220408 |