CN209916218U

CN209916218U - Portable low-temperature insect dissection box

Info

Publication number: CN209916218U
Application number: CN201821988183.2U
Authority: CN
Inventors: 牛东升; 尤士骏; 尤民生; 李金玉; 张�杰
Original assignee: Fujian Agriculture and Forestry University
Current assignee: Fujian Agriculture and Forestry University
Priority date: 2018-11-29
Filing date: 2018-11-29
Publication date: 2020-01-10
Anticipated expiration: 2028-11-29

Abstract

The utility model provides a case is dissected to portable low temperature insect, including the thermostated container, the inside U type frame of having placed of thermostated container, the dish is dissected to low temperature has been placed in the U type frame, the dish is dissected to low temperature includes the base, the base middle part has the recess, fixedly connected with and the same dish body of base upper surface height in the recess, the dish body includes the paraffin layer, the agar layer has been laid to paraffin layer upper end. The utility model discloses the structure is light and handy, easily carries, low cost, and convenient operation dissects and the cold chain transportation to the low temperature of insect when being fit for the field sampling of insect researcher.

Description

Portable low-temperature insect dissection box

Technical Field

The utility model relates to a portable low temperature insect dissects case.

Background

Insect dissection techniques, as a fundamental skill in insect physiology, help every entomologist study insect function and metabolic features at the system, tissue, organ and cell level. The insect dissection tool is an indispensable auxiliary tool for researching insect physiology, products for assisting insect dissection, such as paraffin dissection discs, are available on the market at present, but no professional dissection tool kit and effective method are available for ensuring that the insect 'anaesthesia-dissection-extraction-sample storage' operation is carried out under a low-temperature cold chain, and particularly, the requirement of extracting active substances such as enzymes, proteins and the like in the insect body on the external environment (mainly temperature) is high. In addition, some precious resource insects are difficult to capture in the field or have high mortality rate in long-distance transportation, and lack related cold chain tools for naked eye dissection in any place and time. Therefore, there is a strong need for a portable cold chain dissection kit for insect researchers.

SUMMERY OF THE UTILITY MODEL

The utility model discloses improve above-mentioned problem, promptly the to-be-solved technical problem of the utility model is to provide a portable low temperature insect dissects case, simple structure and convenient to carry.

The utility model discloses a concrete implementation scheme is: the utility model provides a portable low temperature insect dissects case, includes the thermostated container, the inside U type frame of having placed of thermostated container, the dish is dissected to low temperature has been placed in the U type frame, the dish is dissected to low temperature includes the base, the base middle part has the recess, fixedly connected with and the same dish body of base upper surface height in the recess, the dish body includes the paraffin layer, the agar layer has been laid to paraffin layer upper end.

Furthermore, an anatomy tool box is arranged in the side part of the incubator, three layers of drawers are arranged on the side part of the anatomy tool box, one drawer is a scalpel placement box, the other drawer is an anatomy needle placement box, and the other drawer is a positioning needle placement box.

Further, the lateral part of the incubator is also provided with an ice placing box, the bottom of the ice placing box is provided with a plurality of dry ice, the lateral part of the ice placing box is sleeved with an anesthesia box positioned above the dry ice, and the bottom of the anesthesia box is provided with an air vent communicated with the bottom of the ice placing box.

Further, the thermostated container lateral part still overlaps and is equipped with the sample and stores the box, box middle part fixedly connected with centrifuge tube rack is stored to the sample, the centrifuging tube has been placed on the centrifuge tube rack, the box bottom cover is stored to the sample is equipped with the dry ice storage box that is used for placing the dry ice, the box is stored to the dry ice and the inside intercommunication of box is stored to the sample.

Further, have the centrifuging tube that is used for the exhaust cooling on the base and place the hole, the dish body edge still fixedly connected with is the concave body of crescent.

Furthermore, the U-shaped frame comprises a plurality of U-shaped plates, a U-shaped groove is formed in the middle of the upper end of each U-shaped plate, and a space for placing the low-temperature anatomical plate is formed between every two adjacent U-shaped plates.

Furthermore, the thermostat upper end cover is equipped with flip, fixedly connected with temperature sensor on the flip, thermostat front end fixedly connected with and the liquid crystal display that temperature sensor is electric is connected.

Compared with the prior art, the utility model discloses following beneficial effect has: the device has the advantages of reasonable design, simple structure, easy carrying, convenient use and low cost, and is particularly suitable for low-temperature anatomy and cold chain transportation of insects when insect researchers sample in the field.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

FIG. 2 is a schematic structural view of a cryodissection plate according to an embodiment of the present invention;

FIG. 3 is a top view of a cryodissection plate according to an embodiment of the present invention;

FIG. 4 is a schematic structural view of a U-shaped frame according to an embodiment of the present invention;

fig. 5 is a schematic structural view of an anatomical tool box according to an embodiment of the present invention;

fig. 6 is a schematic structural view of a sample storage box according to an embodiment of the present invention.

In the figure: 1-an incubator, 2-a U-shaped frame, 20-a U-shaped plate, 210-a U-shaped groove, 3-a low-temperature dissecting plate, 31-a base, 310-a groove, 311-a centrifuge tube placing hole, 32-a plate-shaped body, 320-a paraffin layer, 321-an agarose layer, 4-a dissecting tool box, 41-a dissecting knife placing box, 42-a dissecting needle placing box, 43-a positioning needle placing box, 5-an ice placing box, 6-a sample storing box, 61-a centrifuge tube rack, 62-a dry ice storing box, 8-a concave body, 9-a flip cover, 10-a temperature sensor and 11-a liquid crystal display screen.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

Embodiment 1 as shown in fig. 1 ~ 6, in the present embodiment, a portable low-temperature insect dissection chamber is provided, which includes an incubator 1, a U-shaped frame 2 is placed inside the incubator 1, a low-temperature dissection plate 3 is placed inside the U-shaped frame 2, the low-temperature dissection plate 3 includes a base 31, a groove 310 is formed in the middle of the base 31, a plate-shaped body 32 having the same height as the upper surface of the base 31 is fixedly connected inside the groove 310, the plate-shaped body 32 includes a paraffin layer 320, and an agar layer 321 is laid on the upper end of the paraffin layer 320.

In this embodiment, the outer layer of the base 31 may be made of soft PVC plastic, the inner layer of the base 31 is made of 304 stainless steel, and the disc-shaped body may be embedded in the groove of the base through a screw structure on the side of the disc-shaped body.

In this embodiment, the base 31 is filled with a coolant and polymer gel; 4/5 the dish-shaped body is highly filled with modified paraffin layer as base layer for anchoring positioning needle for fixing insect, and the rest 1/5 dish-shaped body is provided with agar layer as upper surface for keeping moisture and buffering external force to reduce and protect insect.

In this embodiment, the outside material of thermostated container 1 is EPS, has the advantage of low coefficient of thermal conductivity and buffering striking, and the inside heat preservation that is equipped with of thermostated container 1, heat preservation material are double-deck 304 stainless steel.

In this embodiment, the side of the incubator 1 is provided with the dissecting tool box 4, and the side of the dissecting tool box 4 is provided with three drawers, one of which is a scalpel placing box 41, the other of which is a dissecting needle placing box 42, and the other of which is a positioning needle placing box 43.

In this embodiment, a dissecting needle is placed in the dissecting needle placing box 42, a dissecting knife is placed in the dissecting knife placing box 41, and a positioning needle is placed in the positioning needle placing box 43.

In this embodiment, the lateral part of the incubator 1 is further provided with an ice placing box 5, the bottom of the ice placing box 5 is provided with a plurality of dry ices, the lateral part of the ice placing box 5 is provided with an anesthesia box located above the dry ices in a sleeved mode, and the bottom of the anesthesia box is provided with an air vent communicated with the bottom of the ice placing box 5.

In this embodiment, 1 lateral part of thermostated container is still overlapped and is equipped with

sample storage box

6, 6 middle part fixedly connected with centrifuge tube rack 61 of sample storage box, centrifuge tube rack 61 has placed the centrifuging tube, 6 bottom covers of sample storage box are equipped with the dry ice storage box 62 that is used for placing the dry ice, the dry ice storage box 62 stores the inside intercommunication of box 6 with the sample.

In this embodiment, the base 31 has a centrifugal tube placing hole 311 for exhausting air and cooling, and the edge of the disk-shaped body 32 is further fixedly connected with a crescent concave body 8.

In this embodiment, the U-shaped frame 2 includes a plurality of U-shaped plates 20, a U-shaped groove 210 is formed in the middle of the upper end of each U-shaped plate 20, and a space for placing the cryogenic anatomical disc 3 is formed between adjacent U-shaped plates 20.

In the implementation 2: on the basis of 1 in the implementation, in this embodiment, the pot head is equipped with flip 9 on thermostated container 1, fixedly connected with temperature sensor 10 on flip 9, thermostated container 1 front end fixedly connected with the liquid crystal display 11 that is connected with temperature sensor 10 electricity.

In this embodiment, the temperature sensor may be a pt100 temperature sensor manufactured by wuhan mazize electronic technology limited.

In the embodiment, when the device is used, the U-shaped frame, the low-temperature dissecting plate, the dissecting tool box, the ice placing box, the anesthesia box and the sample storage box are taken out and placed in a refrigerator for precooling (the temperature of the refrigerator is set to be-20 ℃), and the sample storage box is taken out after being frozen for 2 hours and then reassembled; carrying the insect specimen out of the house for use, carrying out anesthesia on the insect after the insect specimen is successfully obtained, selecting a proper anesthesia method according to the activity degree of the insect, generally speaking, if the insect is a larva, putting the larva into an anesthesia box for 10-30 secondsPerforming low-temperature anesthesia; if the adult insect is adult, the adult insect can be placed in an anesthesia box for 30-60 seconds for low temperature and CO₂Anaesthetizing, the dry ice on the bottom of the ice box is sublimated into gas through gasification, the heat of the small anaesthesia box is absorbed and CO is generated₂Gas fills the entire anesthesia box; after anesthesia is finished, the insects are placed on the low-temperature dissecting disk surface, the head is firstly fixed by a positioning needle, the insect body is slowly stretched, and then the tail is fixed, so that the insect body is in a tight state for dissecting and extracting; and finally, transferring the extract into a centrifugal tube filled with 95% ethanol, putting the extract into a sample storage box with the bottom provided with dry ice in advance, bringing the sample storage box to a laboratory, and observing the temperature of a display screen in the whole process. The utility model discloses simple structure, reasonable in design, portable can effectively carry out the low temperature dissection and the cold chain transportation of field sampling, insect to the insect, and convenient and fast has the practicality.

Any technical solution disclosed in the present invention is, unless otherwise stated, disclosed a numerical range if it is disclosed, and the disclosed numerical range is a preferred numerical range, and any person skilled in the art should understand that: the preferred ranges are merely those values which are obvious or representative of the technical effect which can be achieved. Because numerical value is more, can't be exhaustive, so the utility model discloses just disclose some numerical values with the illustration the technical scheme of the utility model to, the numerical value that the aforesaid was enumerated should not constitute right the utility model discloses create the restriction of protection scope.

Also, above-mentioned the utility model discloses if disclose or related to mutually fixed connection's spare part or structure, then, except that other the note, fixed connection can understand: a detachable fixed connection (for example using bolts or screws) is also understood as: non-detachable fixed connections (e.g. riveting, welding), but of course, fixed connections to each other may also be replaced by one-piece structures (e.g. manufactured integrally using a casting process) (unless it is obviously impossible to use an integral forming process).

In addition, the terms used in any aspect of the present disclosure as described above to indicate positional relationships or shapes include similar, analogous, or approximate states or shapes unless otherwise stated.

The utility model provides an arbitrary part both can be assembled by a plurality of solitary component parts and form, also can be the solitary part that the integrated into one piece technology was made.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the same; although the present invention has been described in detail with reference to preferred embodiments, it should be understood by those skilled in the art that: the invention can be modified or equivalent substituted for some technical features; without departing from the spirit of the present invention, it should be understood that the scope of the claims is intended to cover all such modifications and variations.

Claims

1. The utility model provides a case is dissected to portable low temperature insect, a serial communication port, including the thermostated container, the inside U type frame of having placed of thermostated container, the dish is dissected to low temperature has been placed in the U type frame, the dish is dissected to low temperature includes the base, the base middle part has the recess, fixedly connected with and the same dish body of base upper surface height in the recess, the dish body includes the paraffin layer, the agar layer has been laid to paraffin layer upper end.

2. The portable low-temperature insect dissection box according to claim 1, wherein the side part of the incubator is internally provided with a dissection tool box, the side part of the dissection tool box is provided with three layers of drawers, one drawer is a scalpel placement box, the other drawer is a dissection needle placement box, and the other drawer is a positioning needle placement box.

3. The portable low-temperature insect dissection box according to claim 2, wherein an ice placing box is further arranged on the side portion of the incubator, a plurality of dry ice is placed at the bottom of the ice placing box, an anesthesia box located above the dry ice is sleeved on the side portion of the ice placing box, and an air vent communicated with the bottom of the ice placing box is formed in the bottom of the anesthesia box.

4. The portable low-temperature insect dissection box according to claim 3, wherein a sample storage box is further sleeved on the side portion of the incubator, a centrifuge tube rack is fixedly connected to the middle portion of the sample storage box, a centrifuge tube is placed on the centrifuge tube rack, a dry ice storage box for placing dry ice is sleeved on the bottom portion of the sample storage box, and the dry ice storage box is communicated with the inside of the sample storage box.

5. The portable cryogenic insect dissection chamber of claim 1 ~ 4, wherein the base is provided with a centrifugal tube placing hole for cooling, and the edge of the disk-shaped body is fixedly connected with a crescent concave body.

6. The portable low temperature insect dissection chamber of claim 1 ~ 4, wherein the U-shaped frame comprises a plurality of U-shaped plates, each U-shaped plate has a U-shaped groove at the upper middle part, and a space for placing the low temperature dissection disc is arranged between adjacent U-shaped plates.

7. The portable low-temperature insect dissection box according to claim 1 ~ 4, wherein a flip cover is sleeved on the upper end of the incubator, a temperature sensor is fixedly connected to the flip cover, and a liquid crystal display screen electrically connected with the temperature sensor is fixedly connected to the front end of the incubator.