CN109350253A - A kind of Portable low-temp insect anatomy case and its application method - Google Patents
A kind of Portable low-temp insect anatomy case and its application method Download PDFInfo
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- CN109350253A CN109350253A CN201811447588.XA CN201811447588A CN109350253A CN 109350253 A CN109350253 A CN 109350253A CN 201811447588 A CN201811447588 A CN 201811447588A CN 109350253 A CN109350253 A CN 109350253A
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- 241000238631 Hexapoda Species 0.000 title claims abstract description 47
- 210000003484 anatomy Anatomy 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 17
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 18
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000012188 paraffin wax Substances 0.000 claims abstract description 11
- 238000002224 dissection Methods 0.000 claims abstract description 9
- 229920001817 Agar Polymers 0.000 claims abstract description 6
- 239000008272 agar Substances 0.000 claims abstract description 6
- 238000005070 sampling Methods 0.000 claims abstract description 5
- 238000003860 storage Methods 0.000 claims description 35
- 206010002091 Anaesthesia Diseases 0.000 claims description 32
- 230000037005 anaesthesia Effects 0.000 claims description 31
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 28
- 235000011089 carbon dioxide Nutrition 0.000 claims description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 239000004973 liquid crystal related substance Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 3
- 235000019441 ethanol Nutrition 0.000 claims description 3
- 230000002631 hypothermal effect Effects 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 238000005086 pumping Methods 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 3
- 230000003139 buffering effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 241000931526 Acer campestre Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000002309 gasification Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000011232 storage material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
- A61B50/30—Containers specially adapted for packaging, protecting, dispensing, collecting or disposing of surgical or diagnostic appliances or instruments
- A61B50/31—Carrying cases or bags, e.g. doctors' bags
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
- A61D7/04—Devices for anaesthetising animals by gases or vapours; Inhaling devices
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
- A61B2050/001—Temperature-modifying means
- A61B2050/0014—Cooling means
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
- A61B2050/005—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers with a lid or cover
- A61B2050/0051—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers with a lid or cover closable by rotation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
- A61B50/30—Containers specially adapted for packaging, protecting, dispensing, collecting or disposing of surgical or diagnostic appliances or instruments
- A61B2050/3008—Containers specially adapted for packaging, protecting, dispensing, collecting or disposing of surgical or diagnostic appliances or instruments having multiple compartments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
- A61B50/30—Containers specially adapted for packaging, protecting, dispensing, collecting or disposing of surgical or diagnostic appliances or instruments
- A61B50/31—Carrying cases or bags, e.g. doctors' bags
- A61B2050/311—Cases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
- A61D2003/003—Appliances for supporting or fettering animals for operative purposes with head or neck restraining means
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Animal Husbandry (AREA)
- Heart & Thoracic Surgery (AREA)
- Biomedical Technology (AREA)
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Abstract
The present invention provides a kind of Portable low-temp insect anatomy case and its application method, including insulating box, U-shaped frame is placed with inside the insulating box, low temperature dissecting pan is placed in the U-shaped frame, the low temperature dissecting pan includes pedestal, has groove in the middle part of the pedestal, and the identical discoid body with pedestal upper surface height is fixedly connected in the groove, the discoid body includes paraffin layer, and the paraffin layer upper end is equipped with agar layer.Structure of the invention is light and handy, is easy to carry about with one, low in cost, easy to operate, to the dissection of the low temperature of insect and cold chain transportation when being suitble to insect researcher field sampling.
Description
Technical field
The present invention relates to a kind of Portable low-temp insect anatomy case and its application methods.
Background technique
Basic skills of the insect anatomy technology as Entomological Physiology, facilitate each entomology worker can from system,
Insect function and metabolic feature are studied on tissue, organ and cellular level.Insect anatomy tool is research Entomological Physiology
Indispensable auxiliary tool has the product of related supplemental insect dissection currently on the market, and such as paraffin dissecting pan, but there is no specially
Industry dissecting tool case and effective method ensure insect " anesthesia-dissection-extraction-sample storage " operation under low temperature cold chain into
Row, the especially intracorporal enzyme of extraction insect, protein isoreactivity substance require external environment (mainly temperature) high.In addition,
Some precious resources insects are high in the more difficult capture in field or over long distances the transhipment death rate and carry out naked eye dissection everywhere immediately and lack
Weary correlation cold chain tool.Therefore, insect researcher is badly in need of a kind of cold chain dissecting tool case that portability is strong.
Summary of the invention
The present invention improves the above problem, i.e., the technical problem to be solved in the present invention is to provide a kind of portable low
Warm insect anatomy case and its application method, structure are simple and easy to carry.
Specific embodiments of the present invention are: a kind of Portable low-temp insect anatomy case and its application method are provided, including
Insulating box is placed with U-shaped frame inside the insulating box, low temperature dissecting pan, the low temperature dissecting pan packet is placed in the U-shaped frame
Pedestal is included, there is groove in the middle part of the pedestal, the identical discoid body with pedestal upper surface height is fixedly connected in the groove,
The discoid body includes paraffin layer, and the paraffin layer upper end is equipped with agar layer.
Further, dissecting tool box is equipped in the insulating box side, dissecting tool box side is equipped with three layers of pumping
Drawer, wherein a drawer is scalpel holder, it is pilot pin holder there are also a drawer that another drawer, which is dissecting needle holder,.
Further, the insulating box side, which is additionally provided with, sets ice chest, and the ice chest bottom of setting is placed with several blocks of dry ice, institute
It states and sets the anesthesia box that ice chest side is arranged with above dry ice, the anesthesia cassette bottom portion has to be led to set that ice chest bottom be connected to
Stomata.
Further, the insulating box side is also arranged with sample storage box, is fixedly connected in the middle part of the sample storage box
There is centrifuge tube shelf, centrifuge tube is placed on the centrifuge tube shelf, the sample storage box bottom is arranged with for placing dry ice
It is connected to inside dry ice Storage Box, the dry ice Storage Box and sample storage box.
Further, there is the centrifuge tube putting hole for exhaust cooling, the discoid body edge is also solid on the pedestal
Surely it is connected with the concavity body in crescent shape.
Further, the U-shaped frame includes several U-shaped boards, and each U-shaped board upper center has U-type groove, adjacent U-shaped
There is the space for placing low temperature dissecting pan between plate.
Further, the insulating box upper end is arranged with flip lid, and temperature sensor is fixedly connected on the flip lid, described
Insulating box front end is fixedly connected with the liquid crystal display being electrically connected with temperature sensor.
Further, a kind of a kind of application method of Portable low-temp insect anatomy case as claimed in claim 7, including
Steps are as follows: (1) putting low temperature dissecting pan, U-shaped frame, dissecting tool box, scalpel holder, dissecting needle holder, pilot pin
Set box, set ice chest, anesthesia box, sample storage box and dry ice Storage Box take out, be placed into refrigerator, refrigerator be arranged temperature be-
20 °C, placing 2 hours is pre-chilled；After (2) 2 hours, take out low temperature dissecting pan, U-shaped frame, dissecting tool box, solution
Cut open cutter holder, pilot pin holder, sets ice chest, anesthesia box, sample storage box and dry ice Storage Box again at dissecting needle holder
It assembles, carries sampling of setting out；(3) after being collected into insect living body, suitable anesthesia method is selected according to the active degree of insect,
Insect is placed into anesthesia box and is anaesthetized, the dry ice for setting ice chest bottom becomes gas through distillation, and gas absorbs in anesthesia box
Heat and generate CO2 , CO2Gas is full of anesthesia box；(4) under normal circumstances, as fruit insect be larva, put it into anesthesia box
Interior 10s-30s carries out hypothermic anesthesia, if it is adult, then puts it into 30s-60s in anesthesia box and carries out low temperature and CO2Anesthesia；
(5) after the completion of anaesthetizing, insect is taken out, is placed on low temperature dissecting pan, take out the dissecting tool on dissecting tool box, first with fixed
Position needle fixes insect head, slowly stretches polypide, then fixed tail portion, so that polypide is in tensioned state, carried out using scalpel
Dissection is extracted；(6) after the completion of extract extracts, extract is transferred in the centrifuge tube equipped with 95% ethyl alcohol, sample is put it into
On centrifuge tube shelf in Storage Box, sample storage box bottom is placed with dry ice to maintain temperature；(7) bring it back into laboratory or
Designated place, whole process pay attention to observing insulating box internal temperature by liquid crystal display.
Compared with prior art, the invention has the following advantages: present apparatus design is reasonable, structure is simple, is easy to take
Band, it is easy to use, it is low in cost, to the dissection of the low temperature of insect and cold chain transportation when being especially suitable for insect researcher field sampling.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the embodiment of the present invention；
Fig. 2 is low temperature of embodiment of the present invention dissecting pan structural schematic diagram；
Fig. 3 is low temperature of embodiment of the present invention dissecting pan top view；
Fig. 4 is the U-shaped frame structural schematic diagram of the embodiment of the present invention；
Fig. 5 is dissecting tool of embodiment of the present invention box structure schematic diagram；
Fig. 6 is that the embodiment of the present invention sets ice box structure schematic diagram；
Fig. 7 is sample storage box of embodiment of the present invention structural schematic diagram.
In figure: 1- insulating box, 2-U type frame, 20-U template, 210-U type groove, 3- low temperature dissecting pan, 31- pedestal, 310- are recessed
Slot, 311- centrifuge tube putting hole, 32- discoid body, 320- paraffin layer, 321- agar layer, 4- dissecting tool box, 41- scalpel are put
Set box, 42- dissecting needle holder, 43- pilot pin holder, 5- sets ice chest, and 50- anaesthetizes box, 510- venthole, the storage of 6- sample
Box, 61- centrifuge tube shelf, 62- dry ice Storage Box, 7- dry ice, 8- concavity body, 9- flip lid, 10- temperature sensor, 11- liquid crystal display
Screen.
Specific embodiment
The present invention will be further described in detail with reference to the accompanying drawings and detailed description.
Embodiment 1: as shown in Fig. 1 ~ 7, in the present embodiment, providing a kind of Portable low-temp insect anatomy case, including
Insulating box 1 is placed with U-shaped frame 2 inside the insulating box 1, low temperature dissecting pan 3, the low temperature solution is placed in the U-shaped frame 2
Cuing open disk 3 includes pedestal 31, has groove 310 in the middle part of the pedestal 31, is fixedly connected with and table on pedestal 31 in the groove 310
The identical discoid body 32 of face height, the discoid body 32 includes paraffin layer 320, and 320 upper end of paraffin layer is equipped with agar layer
321。
In the present embodiment, 31 outer layer of pedestal can use soft pvc plastic, and 31 internal layer of pedestal uses 304 stainless steels
Material, discoid body can be embedded on base recess by plate-like body side surface screw structural.
In the present embodiment, cold-storage material and high-molecular gel are filled inside pedestal 31；The highly-filled improvement paraffin of 4/5 discoid body
Layer is used as base, can be anchored the pilot pin for fixing insect, and remaining 1/5 discoid body height, as upper surface, is played by agar layer
Moisturizing and buffering external force act on reducing protection polypide.
In the present embodiment, the 1 outside material of insulating box is EPS, has the advantages that low thermal conductivity and buffering collision of closed, permanent
Insulating layer is equipped with inside incubator 1, insulating layer material is double-deck 304 stainless steels.
In the present embodiment, dissecting tool box 4 is equipped in 1 side of insulating box, 4 side of dissecting tool box is equipped with three
Layer drawer, wherein a drawer is scalpel holder 41, it is pilot pin there are also a drawer that another drawer, which is dissecting needle holder 42,
Holder 43.
In the present embodiment, dissecting needle is placed in the dissecting needle holder 42,41 place in the scalpel holder
There is scalpel, is placed with pilot pin in the pilot pin holder 43.
In the present embodiment, 1 side of insulating box, which is additionally provided with, sets ice chest 5, it is described set 5 bottom of ice chest be placed with several pieces it is dry
Ice 7, the anesthesia box 50 setting 5 side of ice chest and being arranged with above dry ice, 50 bottom of anesthesia box have and set ice chest 5
The venthole 510 of bottom connection.
In the present embodiment, 1 side of insulating box is also arranged with sample storage box 6, fixes in the middle part of the sample storage box 6
It is connected with centrifuge tube shelf 61, centrifuge tube is placed on the centrifuge tube shelf 61,6 bottom of sample storage box is arranged with for putting
The dry ice Storage Box 62 of dry ice is set, is connected to inside the dry ice Storage Box 62 and sample storage box 6.
There is the centrifuge tube putting hole 311 for exhaust cooling, the discoid body 32 in the present embodiment, on the pedestal 31
Edge is also fixedly connected with the concavity body 8 in crescent shape.
In the present embodiment, the U-shaped frame 2 includes several U-shaped boards 20, and each 20 upper center of U-shaped board has U-type groove
210, there is between adjacent U-shaped board 20 space for placing low temperature dissecting pan 3.
2 in implementation: on the basis of 1 in implementation, in the present embodiment, 1 upper end of insulating box is arranged with flip lid 9, described
On flip lid 9 it is fixedly connected with temperature sensor 10,1 front end of insulating box is fixedly connected with to be electrically connected with temperature sensor 10
Liquid crystal display 11.
In in this implementation, the temperature sensor can be using the type of the Wuhan maser Electronic Science and Technology Co., Ltd. manufacturing
Number be pt100 temperature sensor.
In the present embodiment, in use, by U-shaped frame, low temperature dissecting pan, dissecting tool box, setting ice chest, anesthesia box and sample
Storage Box is removed and placed into (refrigerator temperature is set as -20 °C) is pre-chilled in refrigerator, is taken out after freezing 2 hours, again group
It installs；Then it goes out to carry to use, after obtaining the success of insect sample, insect is anaesthetized, is selected according to the active degree of insect
Suitable anesthesia is selected, in general, if it is larva, puts it into progress hypothermic anesthesia in 10-30 seconds in anesthesia box；If
It is adult, 30-60 seconds progress low temperature and CO in anesthesia box can be put it into2Anesthesia, the dry ice set on ice chest bottom rise through gasification
Huawei's gas absorbs the heat of anesthesia etui and generates CO2, the entire anesthesia box of gas filling；After the completion of anesthesia, insect is put
It sets in low temperature dissection disk, with pilot pin elder generation fixing head, slowly stretches polypide, then fixed tail portion, be in polypide and tighten
State carries out dissection extraction；Finally, being transferred to extract in the centrifuge tube equipped with 95% ethyl alcohol, places into bottom and be placed in advance
For band to laboratory, whole process pays attention to observation display screen temperature in the sample storage box of dry ice.The configuration of the present invention is simple, design is reasonable,
It is easy to carry, field sampling, the low temperature dissection of insect and cold chain transportation effectively can be carried out to insect, it is convenient and efficient, have practical
Property.
Any technical solution disclosed in aforementioned present invention unless otherwise stated, if it discloses numberical range,
Disclosed numberical range is preferred numberical range, and any it should be appreciated by those skilled in the art preferred numberical ranges
The only obvious or representative numerical value of technical effect in many enforceable numerical value.It, can not since numerical value is more
Exhaustion, so the present invention just discloses technical solution of the component values to illustrate the present invention, also, the above-mentioned numerical value enumerated is not
The limitation to the invention protection scope should be constituted.
Meanwhile if aforementioned present invention discloses or relates to the components or structural member of connection fastened to each other, except another
There is statement outer, is fixedly connected it is to be understood that connection (such as using bolt or screw connection) can be removedly fixed, it can also
To understand are as follows: non-removable to be fixedly connected with (such as riveting, welding), certainly, connection fastened to each other or integral type knot
Structure (such as manufacturing using casting technique is integrally formed) is replaced (obviously can not be using except integrally formed technique).
In addition, for indicating the art of positional relationship or shape applied in any technical solution disclosed in aforementioned present invention
Its meaning includes approximate with its, similar or close state or shape to language unless otherwise stated.
Either component provided by the invention by multiple individual component parts either assembled, or one
The separate part that forming technology manufactures.
Finally it should be noted that: the above embodiments are merely illustrative of the technical scheme of the present invention and are not intended to be limiting thereof；To the greatest extent
The present invention is described in detail with reference to preferred embodiments for pipe, it should be understood by those ordinary skilled in the art that: still
It can modify to a specific embodiment of the invention or some technical features can be equivalently replaced；Without departing from this hair
The spirit of bright technical solution should all cover within the scope of the technical scheme claimed by the invention.
Claims (8)
1. a kind of Portable low-temp insect anatomy case, which is characterized in that including insulating box, be placed with inside the insulating box U-shaped
Frame is placed with low temperature dissecting pan in the U-shaped frame, and the low temperature dissecting pan includes pedestal, has groove, institute in the middle part of the pedestal
It states and is fixedly connected with the identical discoid body with pedestal upper surface height in groove, the discoid body includes paraffin layer, the paraffin
Layer upper end is equipped with agar layer.
2. a kind of Portable low-temp insect anatomy case according to claim 1, which is characterized in that in the insulating box side
Equipped with dissecting tool box, dissecting tool box side is equipped with three layers of drawers, wherein a drawer is scalpel holder, another pumping
Drawer is dissecting needle holder, is pilot pin holder there are also a drawer.
3. a kind of Portable low-temp insect anatomy case according to claim 2, which is characterized in that the insulating box side is also
Equipped with ice chest is set, the ice chest bottom of setting is placed with several blocks of dry ice, and the ice chest side of setting is arranged with above dry ice
Anaesthetize box, the anesthesia cassette bottom portion has and the venthole setting ice chest bottom and be connected to.
4. a kind of Portable low-temp insect anatomy case according to claim 3, which is characterized in that the insulating box side is also
Be arranged with sample storage box, be fixedly connected with centrifuge tube shelf in the middle part of the sample storage box, be placed on the centrifuge tube shelf from
Heart pipe, the sample storage box bottom are arranged with the dry ice Storage Box for placing dry ice, and the dry ice Storage Box and sample store up
Deposit connection inside box.
5. a kind of Portable low-temp insect anatomy case according to claim 4, which is characterized in that have on the pedestal and use
In the centrifuge tube putting hole of cooling, the discoid body edge is also fixedly connected with the concavity body in crescent shape.
6. a kind of Portable low-temp insect anatomy case according to claim 5, which is characterized in that if the U-shaped frame includes
Dry U-shaped board, each U-shaped board upper center have U-type groove, have the sky for placing low temperature dissecting pan between adjacent U-shaped board
Between.
7. a kind of Portable low-temp insect anatomy case according to claim 6, which is characterized in that end cap on the insulating box
Equipped with flip lid, temperature sensor is fixedly connected on the flip lid, the insulating box front end is fixedly connected with and temperature sensor
The liquid crystal display of electrical connection.
8. a kind of a kind of application method of Portable low-temp insect anatomy case as claimed in claim 7, which is characterized in that including
Steps are as follows: (1) putting low temperature dissecting pan, U-shaped frame, dissecting tool box, scalpel holder, dissecting needle holder, pilot pin
Set box, set ice chest, anesthesia box, sample storage box and dry ice Storage Box take out, be placed into refrigerator, refrigerator be arranged temperature be-
20 °C, placing 2 hours is pre-chilled；After (2) 2 hours, take out low temperature dissecting pan, U-shaped frame, dissecting tool box, solution
Cut open cutter holder, pilot pin holder, sets ice chest, anesthesia box, sample storage box and dry ice Storage Box again at dissecting needle holder
It assembles, carries sampling of setting out；(3) after being collected into insect living body, suitable anesthesia method is selected according to the active degree of insect,
Insect is placed into anesthesia box and is anaesthetized, the dry ice for setting ice chest bottom becomes gas through distillation, and gas absorbs in anesthesia box
Heat and generate CO2 , CO2Gas is full of anesthesia box；(4) under normal circumstances, as fruit insect be larva, put it into anesthesia box
Interior 10s-30s carries out hypothermic anesthesia, if it is adult, then puts it into 30s-60s in anesthesia box and carries out low temperature and CO2Anesthesia；
(5) after the completion of anaesthetizing, insect is taken out, is placed on low temperature dissecting pan, take out the dissecting tool on dissecting tool box, first with fixed
Position needle fixes insect head, slowly stretches polypide, then fixed tail portion, so that polypide is in tensioned state, carried out using scalpel
Dissection is extracted；(6) after the completion of extract extracts, extract is transferred in the centrifuge tube equipped with 95% ethyl alcohol, sample is put it into
On centrifuge tube shelf in Storage Box, sample storage box bottom is placed with dry ice to maintain temperature；(7) bring it back into laboratory or
Designated place, whole process pay attention to observing insulating box internal temperature by liquid crystal display.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811447588.XA CN109350253B (en) | 2018-11-29 | Portable low-temperature insect dissecting box and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811447588.XA CN109350253B (en) | 2018-11-29 | Portable low-temperature insect dissecting box and use method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109350253A true CN109350253A (en) | 2019-02-19 |
| CN109350253B CN109350253B (en) | 2024-06-25 |
Family
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110279483A (en) * | 2019-06-27 | 2019-09-27 | 山东科技大学 | Experiment porch is used in a kind of operation of insect |
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