CN203369308U - Vitrification refrigeration carrier of biology samples and combined device for biology sample cryopreservation - Google Patents
Vitrification refrigeration carrier of biology samples and combined device for biology sample cryopreservation Download PDFInfo
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- CN203369308U CN203369308U CN201320175438.4U CN201320175438U CN203369308U CN 203369308 U CN203369308 U CN 203369308U CN 201320175438 U CN201320175438 U CN 201320175438U CN 203369308 U CN203369308 U CN 203369308U
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Abstract
The utility model provides a vitrification refrigeration carrier of biology samples and a combined device for biology sample cryopreservation. The carrier includes a main body and a sealing cap. The closer end of the main body is sealed with the internal bottom surface of the sealing cap to form an enclosed refrigeration sample placing area. The refrigeration sample placing area is provided with a refrigeration sample placing platform. The main body is provided with a refrigeration liquid flow channel, and the sealing cap is provided with a liquid guiding hole corresponding to the refrigeration liquid flow channel, so that refrigeration liquid which flows in the refrigeration liquid flow channel can contact with the external surface of the refrigeration sample placing area and cool the biology samples in the refrigeration sample placing area, when the biology samples are refrigerated. The vitrification refrigeration carrier of the biology samples has advantages of being safe, being non-poisonous, being high in efficiency of vitrification refrigeration and rewarming operation, and being wide in application prospect in the field of biology sample refrigeration.
Description
Technical field
The utility model relates to Frozen Biological device field, particularly, is included as in the auxiliary procreation technology field and provides safe and effective, operates easy closed freezing carrier and the frozen combination unit of biological specimen for aseptic glass freezing embryo or egg cell.
Background technology
In the human assistance reproductive technology, put into the liquid nitrogen cryopreservation of-196 ℃ by a plurality of embryos of obtaining after in vitro fertilization or from a plurality of ovums that take out in women's body, for short-term or the long-term embryo transplantation of selecting a time, become and improve common method in vitro fertilization, Success Rate of Embryo Transfer.But its clinical meaning of glass freezing biological specimen be cooling agent as liquid nitrogen in longer-term storage, embryo or the cell frozen through cooling agent can stasi also can be preserved many decades, the secondth, frozen biological specimen, after rewarming, can recover the living cells on one or more biological significances.
When the normal temperature material is frozen; normal temperature material and utmost point cryogenic coolant; the Leiden Dan Frost effect produced while meeting as liquid nitrogen; can form and intercept heat conduction steam film at frozen material surface; and the cooling rate of the required frozen material that slows down; generally need to increase the formation that the cryoprotection agent concentration prevents ice crystal in the biological specimen refrigerating process; and the high concentration cryoprotector is to biological specimen tool toxic; cell quality after causing recovering reduces; therefore, the vitrification of pursuit snap frozen is developed.
At present, in order to accelerate freezing rate, the more general all employings of vitrified frozen vector both at home and abroad are open, and stretching straw method, quartz capillary method, Cryoloop method, Cryotip method etc. are arranged.2005, Kuwayama proposed to improve by minimizing liquor capacity new method--the Cryotop method etc. of cooldown rate, and the freezing carrier that is about to be loaded with sample directly immerses in liquid nitrogen.But because biological specimen directly contacts with liquid nitrogen; the gnotobasis that can not provide biological specimen to store; there is cross contamination risk; and liquid nitrogen is to the direct toxic action of cell also not measurable (Covo A; Domingo J, Perez S, et al.Vitrification:an effective new approach to oocyte banking and preserving fertility in cancer patients[J] .Clin Transl Oncol; 2008,10 (5): 268-273; Closed straw vitrifying freeze process is observed for the human body early embryo refrigerating effect, Shandong 2010 the 15th phases of the volume end of the year 50 of medicine; ), and research recently more shows, low molecular compound in liquid nitrogen has toxicity (Y Panagiotidis to biological specimen, P Vanderzwalmen, Y Prapas, E kasapi, et, al Open versus closed vitrification of blastocysts from an oocyte-donation programme:a prospective randomized study.Reproductive BioMedicine Online, In Press, Uncorrected Proof, Mar2013).Patent application (200710192245.9) discloses a kind of closed type glass freezing device that stops external source pathogenic material to pollute biological specimen, although but accomplished the closed system of isolating with cooling agent, but, due to various steps such as needs thermoplastic sealings, the operation of the freezing carrier of the type is still aobvious complicated.
Therefore, in the urgent need to developing, a kind of glass freezing efficiency is high, easy to operate, the biological sample closed refrigerating plant of safety non-toxic in this area.
The utility model content
The highly-efficient glassization that the utility model provides is freezing and operate the airtight save set of easy biological specimen.
The utility model provides a kind of biological specimen vitrified frozen vector, described carrier comprises body and sealing cap, one airtight freezing sample rest area is formed on the near-end of wherein said body and sealing cap bottom sealing mutually, and described freezing sample rest area is provided with a freezing sample placement platform
And described body is provided with coolant flow channel, and described sealing cap is provided with the pod apertures corresponding with described coolant flow channel, make and carry out biological specimen when freezing, the cooling agent circulated in described passage with the outer surface of described freezing sample rest area, contact and cooling described freezing sample rest area in biological specimen.
In another preference, the frozen liq circulated in described passage directly contacts with the outer surface of described freezing sample placement platform, thus the cooling biological specimen be positioned on described freezing sample placement platform.
In another preference, carrying out biological specimen with the biological specimen vitrified frozen vector when freezing, mobile freezant outside described vitrified frozen vector, with the outer surface of described freezing sample placement platform, directly contact, thus the cooling biological specimen be positioned on described freezing sample placement platform.
In another preference, also be provided with seal washer between described body proximal end and sealing cap.
In another preference, the flat surfaces that described freezing sample placement platform is a suitable sample size;
And be arranged at the top of being located at described body proximal end, or be fixed in the top of described body proximal end;
Or be arranged at the bottom (especially inner bottom surface) of described sealing cap; Or be fixed in the bottom of described sealing cap.
In another preference, the bottom of described sealing cap is inner bottom surface.
In another preference, described freezing sample placement platform and described body proximal end end face are in same level; Or described freezing sample placement platform is lower than described body proximal end end face level.
In another preference, described freezing sample rest area height is 0.5-5mm
In another preference, that described placement platform molding mode comprises is one-body molded with carrier body or sealing cap, bonding forming, snapping moulding.
In another preference, described biological specimen comprises: cell, tissue, organ.
In another preference, described cell comprises egg cell, embryonic cell.
In another preference, described body also comprise the holding part of body distal end and between body proximal end and far-end, for the engaging piece with the sealing cap sealed engagement.
In another preference, described passage comprises the distal openings that is positioned at described holding part and the side opening of being located at described body sidewall, and described distal openings and described side opening are connected.
In another preference, described distal openings extends to described body proximal end from described holding part to described body central inner, with freezing sample placement platform, jointly forms the described body that far-end is provided with opening hollow.
In another preference, the side opening of described body sidewall is between described freezing sample rest area and described engaging piece, and more preferably, the side opening of described body sidewall is close to described freezing sample rest area.
In another preference, the side opening of described body sidewall is at least 2, more preferably, is 4-6.
In another preference, described engaging piece surface is provided with for the movable secure component with the airtight engagement of sealing cap.
In another preference, described movable secure component comprises screw-tightened parts, socket secure component, snapping secure component.
In another preference, the outer end face of described sealing cap or side are provided with a sample information flag district.
In another preference, described sealing cap has different color, in order to distinguish embryo, the different classes of cell or tissue of different growth cycles.
In another preference, described platform diameter 1-15mm, preferably, be 3-12mm, and that better is 5-10mm; Best, be 6-8mm; And/or
Described land thickness is 0.06-0.15mm, preferably, is 0.07-0.12mm, more preferably 0.08-0.10mm; And/or
Described biological specimen rest area height is 0.5-5mm, and preferably 1-4mm, be more preferably 2-3mm.
Be the freezing rate of described carrier after placing biological specimen at least 10000-20000 ℃/minute in another preference? Preferably, be 30000-50000 ℃/minute; More preferably, be 60000-100000 ℃/minute.
In another preference, described body and sealing cap material are identical or different, and are selected from lower group: macromolecular material, metal; And/or
Described carrier platform material is selected from lower group: macromolecular material, metal.
In another preference, described macromolecular material comprises the PE(polyethylene), HDPE (high density polyethylene (HDPE)); The PP(polypropylene); The PET(PETG);
In another preference, described metal comprises stainless steel, titanium alloy.
In another preference, described macromolecular material or metal are medical macromolecular materials or medical metal.
In another preference, described holding part diameter 6-15mm, described distal openings diameter 2-10mm; And/or
The size of described base side opening is 1-3mm * 1-3mm; And/or
Described sealing cap pod apertures size is 2-5mm * 2-5mm.
In another preference, described frozen liq circulation passage is stepped, go the tip circle column.
In another preference, described base side opening can be circular port, elliptical aperture, irregularly-shaped hole or flat hole.
In another preference, described base side opening is at least 2, is more preferably 4-6, and described sealing cap pod apertures is at least 2, is more preferably 4-6.
The utility model second aspect, provide a kind of closed vitrification method of biological specimen, comprises step:
A) provide the utility model first aspect described biological specimen vitrified frozen vector, described carrier comprises body and sealing cap, and by described biological specimen, be positioned on the freezing sample placement platform of described biological specimen vitrified frozen vector, thereby form the carrier body containing biological specimen;
B) carrier body and the described sealing cap containing biological specimen of step a) are meshed and tighten, thereby form the seal carrier containing biological specimen;
C) seal carrier containing biological specimen of step b) is carried out to the sample information mark in mark zone;
D) seal carrier that step c) has been marked with to biological specimen information is inserted in cooling agent and is preserved.
In another preference, the closed vitrification method of described biological specimen also comprises that the seal carrier to containing biological specimen carries out mark; And/or freezing carrier is carried out placing biological specimen and being sealed after mark, thereby obtain the biological specimen seal carrier of mark.
The utility model third aspect, provide a kind of biological specimen frozen combination unit, and described combination unit comprises the described carrier of one or more the utility model first aspects; Liquid nitrogen; With the liquid nitrogen storage device.
The utility model also provides the purposes of the described biological specimen closed of a kind of the utility model first aspect vitrified frozen vector, for biological specimen being carried out to airtight freezing preservation.
In should be understood that in the utility model scope, above-mentioned each technical characterictic of the present utility model and can combining mutually between specifically described each technical characterictic in below (eg embodiment), thus form new or preferred technical scheme.As space is limited, tire out and state no longer one by one at this.
The accompanying drawing explanation
Fig. 1 has shown the assembling schematic diagram of the utility model biological specimen vitrified frozen vector one.
Fig. 2 has shown the assembling schematic diagram of the utility model biological specimen vitrified frozen vector two.
Fig. 3 has shown the assembling schematic diagram of the utility model biological specimen vitrified frozen vector three.
Wherein, Reference numeral is corresponding each component names of carrier and function are as follows:
1 frozen liq circulation passage; 2 pod apertures; 4 side openings; 5 distal openings; 10 sealing caps; 11 sample labeling districts; 12 holding parts; 13 far-ends; 20 freezing sample placement platforms; 22 sealing rings; 23 freezing sample rest areas; 24 near-ends.
Embodiment
The inventor, through extensive and deep research, provides a kind of carrier for the airtight freezing preservation of biological sample and device first.Evidence, the designed coolant flow channel of the utility model carrier, cooling agent pod apertures and Frozen Biological platform are when making the airtight isolation of biological sample and freezing liquid, still can reach efficient glass freezing effect, after rewarming, survival rate of embryo is high, and simple to operate.On this basis, completed the utility model.
Freezing sample placement platform and rest area
The freezing sample rest area that the formation one sealed to each other of the inner bottom surface of the utility model body proximal end and the utility model sealing cap is airtight, and described freezing sample rest area is provided with a freezing sample placement platform.
Described platform can be fixedly connected on the end face of described body proximal end, with described body proximal end, jointly forms the hollow body structure that near-end seals.Wherein, the plane of described platform can be with the end face of described body proximal end in same level, or lower than the end face of body proximal end.And described platform can be fixed by the mode that is fixedly connected with and the body proximal end end face of various routines, usually, can be for body moulded section, be connected with body proximal end by the macromolecule bonding material, or connect and be fastened on body proximal end by snapping.A kind of preferred connected mode is and body moulded section.
Described platform can also be fixedly connected on the inner bottom surface of described sealing cap.When described platform, with after described sealing cap is connected, described body back-off enters sealing cap, and chimeric by body proximal end and platform, and forms airtight freezing sample rest area.
Usually, Zhou Jing along described freezing sample rest area, be equipped with sealing ring, for the space between airtight body proximal end and sealing cap, ensure that freezing sample rest area reaches airtight, effective contacting of isolation biological specimen and cooling agent, thus prevent cross pollution and the murder by poisoning to biological specimen of toxicants such as unknown pathogene in freezant.
The material of the freezing sample placement platform of the utility model is nontoxic and can tolerates medical macromolecular materials, the metal of quenching and shock heating variations in temperature.Usually, the material that can be used for the freezing sample placement platform of the utility model comprises PE, HDPE, PET, PP, medical stainless steel, medical titanium alloy.
It is characterized in that of the freezing sample placement platform of the utility model, described platform diameter 1-15mm, preferably, be 3-12mm, and that better is 5-10mm; Be 6-8mm best; And/or the thickness of carrier platform is 0.06-0.15mm, preferably, be 0.07-0.12mm, more preferably 0.08-0.10mm.
In addition, the size of the utility model biological specimen vitrified frozen vector can change according to the required freezing concrete size of biological specimen and the operation removed of unnecessary cryoprotector.Usually, the diameter of freezing carrier platform can be 1-15mm, height 0.5-5mm.
The freezing liquid circulation passage
Can be used for coolant flow channel of the present utility model and comprise following part:
(a) be positioned at the distal openings of body distal end;
(b) be positioned at the side opening of body proximal end;
(c) be positioned at sealing cap sidewall, the pod apertures corresponding with body proximal end side opening position.
Wherein, described distal openings, side opening are interconnected via the body central inner, and corresponding to the pod apertures that is positioned at described sealing cap sidewall.Described side opening is positioned at body proximal end, and more preferably, described side opening is close to freezing sample placement platform.
Usually, described side opening and pod apertures are at least respectively two, are more preferably four or more.
In the utility model, after sealing cap and the mutual closing seam of body, insert in frozen liq, frozen liq can be exchanged by distal openings-side opening-pod apertures circulation through the freezing liquid circulation passage, so, and the process of ANALYSIS OF COOLANT FLOW, can make the biological specimen carrier platform surface carry out precooling, enough thin due to carrier platform, thus the leidenfrost effect reduced, and the tissue samples that makes to be positioned at freezing placement platform is cooling rapidly.
In the utility model, described base side opening diameter 1-3mm, or the oval transverse holes of 2 * 3mm, the oval transverse holes that described sealing cap pod apertures diameter is 2-5mm * 2-5mm.
The three-dimensional shape that can be used for distal openings of the present utility model or base side opening or pod apertures is not particularly limited, can for any be conducive to freezant circulation, the shape of coupling in correspondence with each other, usually, in the utility model the three-dimensional shape of each opening or pod apertures stepped, go the tip circle column.
The method of snap frozen biological specimen
The method of snap frozen biological specimen in the utility model comprises step:
A) provide claim 1 described biological specimen vitrified frozen vector, described carrier comprises body and sealing cap, and by described biological specimen, be positioned on the freezing sample placement platform of described biological specimen vitrified frozen vector, thereby form the carrier body containing biological specimen;
B) carrier body and the described sealing cap containing biological specimen of step a) are meshed and tighten, thereby form the seal carrier containing biological specimen;
C) seal carrier containing biological specimen of step b) is carried out to the sample information mark in mark zone;
D) seal carrier that step c) has been marked with to biological specimen information is inserted in cooling agent and is preserved.
Usually, the closed vitrification method of described biological specimen also comprises that the seal carrier to containing biological specimen carries out mark; And/or freezing carrier is carried out placing sample and being sealed after mark, thereby obtain the seal carrier of mark.
Use the method for the utility model vitrified frozen vector and snap frozen biological specimen, can reach the cooldown rate of 20000 ℃/minute, more preferably, the cooldown rate of 40000-100000 ℃/minute, obtain different cooldown rates and biological specimen vitrifying effect depending on adopting carrier platform different materials or size.
In addition, the carrier in the utility model, when being applied to experiment or diagnosis and treatment, need carrying out after asepticize is processed using, and keep gnotobasis in operating process.
Material and outward appearance
The material that can be used for preparing the utility model biological specimen vitrified frozen vector is nontoxic, and through aseptic process, and can tolerate macromolecular material or the metal of quenching and shock heating variations in temperature.
In another preference, described macromolecular material comprises the PE(polyethylene), HDPE (high density polyethylene (HDPE)); The PP(polypropylene); The PET(PETG).
In another preference, described metal comprises stainless steel, titanium alloy.
In another preference, described macromolecular material or metal are medical macromolecular materials or medical metal.
In the utility model, body fastens sealing with sealing cap mutually in comings and goings socket modes such as mutual snapping or screw thread rotation are connected.Usually, described snapping or screw thread rotation jockey can reach by various conventional methods the effect of sealing, as increased the sealing ring can tolerate quenching and shock heating variations in temperature, as the form that changes screw thread or angle etc., the enclosed self-locking of assurance obturator in the suddenly cold and hot process.
Body in the utility model and the face shaping of sealing cap are not particularly limited, can be for any cylindricality outward appearance matched each other, and as cylindrical, hexagonal cylindricality, thereby the convenient operation person holds and carrier stable when placing the biological specimen such as embryo, ovum.In addition, body of the present utility model and sealing cap can adopt various different colors, to distinguish the frozen sign of carrier for different biological specimens, tissue, cell etc.
The beneficial effects of the utility model:
1. biological specimen, safety non-toxic are preserved in sealing: the utility model carrier and method for biological specimen and frozen liq are isolated, airtight preservation, safety non-pollution, reduced the genotoxic potential danger of frozen liq to biological specimen.
2. freezing rate is fast, reach highly-efficient glassization freezing: adopt the utility model carrier and method can reach the freezing rate of 10000-100000 ℃/minute, depending on adopting carrier platform different materials or size, obtain different cooldown rates and biological specimen vitrifying effect.Thereby play the impact that rapid glassization is freezing and avoid in the biological specimen refrigerating process being subject to ice crystal to destroy.
3. easy to operate: the utility model carrier adopts screw threads for fastening or socket, the buckled sealing means such as fastening, after only needing during operation biological specimen is positioned over to platform, tightens sealing cap, can put into liquid nitrogen to store, and has replaced the complicated process of traditional thermoplastic sealing.
4. safe and effective: as due to the special construction of this device and operate simple and easyly, to take out after sample heats up and load general conveniently as sample, be difficult for the loss of generation biological specimen after heating up.
Embodiment 1: the sealing property test
Respectively get 50 cover closed vitrified frozen vectors one (as Fig. 1) and carrier three (as Fig. 3), after sterilizing, put into respectively liquid nitrogen after one day, one week, 30 days, 60 days, 90 days, take out in liquid nitrogen, drop into rapidly in 37 ℃ of water, place 10 minutes, be put on filter paper and stand the several seconds after taking-up, open sealing cap, with clean filter paper wiping placement platform, the whether watermark of Microscopic observation filter paper, result is as shown in table 1:
Table 1
Result shows, this device airtight performance reaches 100%.
Embodiment 2: ovum or embryo survival
1. experimental subjects
Laboratory animal is clean level kunming mice (purchased from Shanghai Vaccine and Serum Institute), and female is 9 week age, 29-33g/, and totally 80,
Male is 9 week age, and only, totally 20, illumination adopts the 12h light and shade alternately to body weight 38-45g/.
2 experimental techniques:
1) oocytes collection: short neck is put to death super ovulation induction sexual maturity Kunming female mice and is separated oviduct, get oolemma complete, the egg mother cell that the matrix refractivity is good (oolemma and after birth not damaged, the all gaps of ovum are clear, without kytoplasm, leak outside or the egg cell atrophy, normal in size, the refractive power of endochylema homogeneous is good).
2) sperm is collected: be fertilized the same day, disconnected post is put to death healthy male mice and is obtained seminal fluid.
3) in vitro fertilization: with 2 * 10
6/ ml extractive liquids in different consistencies and ovum are hatched jointly, take out 24 hours 37 ℃ of 5%CO of ovum after 5 hours
2after cultivating in incubator, observe the formation of two prokaryotics.
4) freezing carrier: respectively get 80 covers the utility model freezing carrier one (Fig. 1), two (Fig. 2), three (Fig. 3) after sterilizing, load and select after qualified protokaryon embryo in liquid nitrogen frozen, within 3 days, take out afterwards, rewarming, cultivate, and observes its embryo's division situation.
Wherein, 2 cell stage numbers are that fertilization cell is cultivated the survival 2 somatoblast embryo numbers that obtain in latter 1 day; 4 cell number are that fertilization cell is cultivated the survival 4 somatoblast embryo numbers that obtain after latter 48 hours; The blastaea number is that fertilization cell is cultivated the cell number that enters blastula stage obtained in latter 96 hours.
3. the utility model device is as shown in table 2 to the result of Mouse Pronuclear embryo cryopreservation:
Table 2
| Freezing carrier | Protokaryon embryo number (individual) | 2 cell rates (%) | 4 cell rates (%) | Blastaea rate (%) |
| Device one | 80 | 75.00（60/80） | 65.00(39/60) | 51.67(31/60) |
| Device two | 80 | 81.25(65/80) | 72.30(47/65) | 66.15(43/65) |
| Device three | 80 | 83.75(67/80) | 76.12(51/67) | 73.13(49/67) |
Annotate: 2 cell rates=2 cell stage numbers/protokaryon embryo number; 4 cell rates=4 cell stage numbers/2 cell stage numbers; Blastaea rate=blastaea number/2 cell stage numbers.
5. conclusion
In the art, while using opening or closed freezing carrier to carry out glass freezing to biological specimen (embryo), the blastaea rate is about 50%-70%.From the result of the present embodiment, use the utility model carrier to carry out the glass freezing embryo, the blastaea rate is respectively 51.67%, 66.15% and 73.13%, and the blastaea rate of its result and other apparatus and method of this area is close or even more excellent.In addition, the utility model carrier also has advantages of simple to operate, safe, therefore, can substitute conventional apparatus and method, is suitable for extensive use.
All documents of mentioning at the utility model are all quoted as a reference in this application, just as each piece of document quoted separately as a reference.Should be understood that in addition those skilled in the art can make various changes or modifications the utility model after having read above-mentioned instruction content of the present utility model, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (10)
1. a biological specimen vitrified frozen vector, it is characterized in that, described carrier comprises body and sealing cap, and an airtight freezing sample rest area is formed on the near-end of wherein said body and sealing cap bottom sealing mutually, described freezing sample rest area is provided with a freezing sample placement platform
And described body is provided with coolant flow channel, and described sealing cap is provided with the pod apertures corresponding with described coolant flow channel, make and carry out biological specimen when freezing, the cooling agent circulated in described passage with the outer surface of described freezing sample rest area, contact and cooling described freezing sample rest area in biological specimen.
2. carrier as claimed in claim 1, is characterized in that, the flat surfaces that described freezing sample placement platform is a suitable sample size; And be arranged at the top of being located at described body proximal end, or be fixed in the top of described body proximal end; Or
Be arranged at the bottom of described sealing cap; Or be fixed in the bottom of described sealing cap.
3. carrier as claimed in claim 2, is characterized in that, the bottom of described sealing cap is inner bottom surface.
4. carrier as claimed in claim 1, is characterized in that, described body also comprise the holding part of body distal end and between body proximal end and far-end, for the engaging piece with the sealing cap sealed engagement.
5. carrier as claimed in claim 4, is characterized in that, described passage comprises the distal openings that is positioned at described holding part and the side opening of being located at the sidewall of described body, and described distal openings and described side opening are connected.
6. carrier as claimed in claim 4, is characterized in that, described engaging piece surface is provided with for the movable secure component with the airtight engagement of sealing cap.
7. carrier as claimed in claim 1, is characterized in that, described platform diameter 1-15mm; And/or
Described land thickness is 0.06-0.15mm; And/or
Described biological specimen rest area height is 0.5-5mm.
8. carrier as claimed in claim 5, is characterized in that, described holding part diameter 6-15mm, described distal openings diameter 2-10mm; And/or
The size of described base side opening is 1-3mm * 1-3mm; And/or
Described sealing cap pod apertures size is 2-5mm * 2-5mm.
9. carrier as claimed in claim 5, is characterized in that, described base side opening is at least 2, and described sealing cap pod apertures is at least 2.
10. the frozen combination unit of biological specimen, is characterized in that, described combination unit comprises
One or more carriers claimed in claim 1; Liquid nitrogen; With
The liquid nitrogen storage device.
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| CN201320175438.4U CN203369308U (en) | 2013-04-09 | 2013-04-09 | Vitrification refrigeration carrier of biology samples and combined device for biology sample cryopreservation |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105163579A (en) * | 2013-04-09 | 2015-12-16 | 楼伟 | Biological sample vitrification carrier and usage thereof |
| CN106459862A (en) * | 2014-06-30 | 2017-02-22 | 三菱制纸株式会社 | Jig for vitrification preservation of cells or tissues |
| US10624335B2 (en) | 2014-10-23 | 2020-04-21 | Mitsubishi Paper Mills Limited | Tool for cryopreservation of cell or tissue and cryopreservation method |
| CN111700061A (en) * | 2020-06-12 | 2020-09-25 | 山东大学齐鲁医院 | Closed vitrification carrier and application thereof |
| CN113016776A (en) * | 2019-12-24 | 2021-06-25 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
| CN114532329A (en) * | 2017-04-21 | 2022-05-27 | 富士胶片欧文科技有限公司 | Vitrification device and method for preparing a sample |
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2013
- 2013-04-09 CN CN201320175438.4U patent/CN203369308U/en not_active Expired - Lifetime
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105163579A (en) * | 2013-04-09 | 2015-12-16 | 楼伟 | Biological sample vitrification carrier and usage thereof |
| EP2984928A4 (en) * | 2013-04-09 | 2017-01-11 | Wei Lou | Biological sample vitrification carrier and usage thereof |
| CN105163579B (en) * | 2013-04-09 | 2018-01-05 | 楼伟 | A kind of biological sample vitrification carrier and application thereof |
| CN106459862A (en) * | 2014-06-30 | 2017-02-22 | 三菱制纸株式会社 | Jig for vitrification preservation of cells or tissues |
| US10039278B2 (en) | 2014-06-30 | 2018-08-07 | Mitsubishi Paper Mills Limited | Device for vitrification preservation of cells or tissues |
| CN106459862B (en) * | 2014-06-30 | 2018-12-18 | 三菱制纸株式会社 | The vitrificated cryopreserration fixture of cell or tissue |
| US10624335B2 (en) | 2014-10-23 | 2020-04-21 | Mitsubishi Paper Mills Limited | Tool for cryopreservation of cell or tissue and cryopreservation method |
| CN114532329A (en) * | 2017-04-21 | 2022-05-27 | 富士胶片欧文科技有限公司 | Vitrification device and method for preparing a sample |
| CN114532329B (en) * | 2017-04-21 | 2023-11-28 | 富士胶片欧文科技有限公司 | Vitrification device and method for preparing a sample |
| CN113016776A (en) * | 2019-12-24 | 2021-06-25 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
| CN111700061A (en) * | 2020-06-12 | 2020-09-25 | 山东大学齐鲁医院 | Closed vitrification carrier and application thereof |
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