CN209348806U - Thallus sample broke mechanism - Google Patents
Thallus sample broke mechanism Download PDFInfo
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- CN209348806U CN209348806U CN201822007880.1U CN201822007880U CN209348806U CN 209348806 U CN209348806 U CN 209348806U CN 201822007880 U CN201822007880 U CN 201822007880U CN 209348806 U CN209348806 U CN 209348806U
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Abstract
The utility model relates to a kind of thallus sample broke mechanisms, including sample introduction cup, peristaltic pump, cooling cycle machine, high pressure homogenizer and receipts sample cup, cooling cycle machine is equipped with cold rinse bank, sample cell is equipped in cold rinse bank, sample cell is helical structure, high pressure homogenizer includes body, body is equipped with sample introduction tank, crushing device, sample introduction cup, peristaltic pump, sample cell, sample introduction tank, crushing device, sample cup is received to be sequentially connected, the outside of cold rinse bank is equipped with the first cold liquid channel, the outside of crushing device is equipped with the second cold liquid channel, the liquid outlet in the first cold liquid channel is connect with the inlet in the second cold liquid channel, the inlet in the first cold liquid channel is connect with the liquid outlet in the second cold liquid channel.The utility model structure is simple, crushing efficiency is high while can guarantee thallus downstream biological activity of albumen again.
Description
Technical field
The utility model relates to bacterial cell disruption technical fields, more particularly to a kind of thallus sample broke mechanism.
Background technique
The inside temperature of the high-pressure homogeneous case of reduction only simple when in the prior art for BL21-FMDV-B4 bacterial cell disruption
Degree, there is no the temperature for inherently reducing BL21-FMDV-B4 thallus, therefore BL21-FMDV-B4 thallus is in shattering process
Big calorimetric is generated by high-speed impact, causes BL21-FMDV-B4 thallus downstream albumen to lose biological activity, if in order to reduce
The big calorimetric generated during BL21-FMDV-B4 bacterial cell disruption, then must reduce the cracking pressure of high pressure homogenizer, thus again
Reduce the crushing efficiency of high pressure homogenizer.
Utility model content
That the technical problem to be solved by the present invention is to provide a kind of structures is simple, crushing efficiency is high while can guarantee bacterium again
The thallus sample broke mechanism of body downstream biological activity of albumen.
A kind of thallus sample broke of the utility model mechanism, including it is sample introduction cup, peristaltic pump, cooling cycle machine, high-pressure homogeneous
Machine and receipts sample cup, the cooling cycle machine are equipped with cold rinse bank, and sample cell is equipped in the cold rinse bank, and the sample cell is spiral
Structure, the high pressure homogenizer include body, the body be equipped with sample introduction tank, crushing device, the sample introduction cup, peristaltic pump,
Sample cell, sample introduction tank, crushing device, receipts sample cup are sequentially connected, and the outside of the cold rinse bank is equipped with the first cold liquid channel, described broken
The outside of crushing device is equipped with the second cold liquid channel, the liquid outlet in the first cold liquid channel and the feed liquor in the described second cold liquid channel
Mouth connection, the inlet in the first cold liquid channel are connect with the liquid outlet in the described second cold liquid channel.
Preferably, the peristaltic pump include pump case, the rotor that is arranged in pump case, the end of the rotor
It is uniformly equipped with roll along its circumferencial direction, the roll is positioned close on the periphery of rotor, the top of the pump case
For arcuate structure, the hose of the peristaltic pump wears the channel formed between the pump case and rotor.
It being preferably in any of the above-described scheme, the hose on the peristaltic pump, one end extends in the sample introduction cup,
The other end is connect with the sample cell.
It is preferably in any of the above-described scheme, the crushing device includes crusher chamber, and partition, institute are equipped in the crusher chamber
It states partition and crusher chamber is separated into upper cavity, lower chamber, the upper cavity is equipped with agitating device, and the lower chamber is equipped with spiral transferring
The center of expects pipe, supersonic generator, the partition is equipped with conveying mouth, and the upper end of the spiral conveying pipeline is connect with conveying mouth,
The lower end of the spiral conveying pipeline is connected with screw conveyor, and the supersonic generator is fixed on the inner wall of lower chamber, institute
The top for stating crusher chamber is equipped with feed inlet, and the feed inlet is connect with sample introduction tank, the discharge end and receipts sample of the screw conveyor
Cup connection.
It is preferably in any of the above-described scheme, the partition is in inverted cone-shaped structure.
It is preferably in any of the above-described scheme, the agitating device includes stirring motor, agitating shaft and stirring blade, described
Stirring motor is fixed on the top of crusher chamber, the output end and stirring axis connection of the stirring motor, the stirring blade setting
In the outside of agitating shaft.
It is preferably in any of the above-described scheme, the inner wall of the body is equipped with noise reduction layer, and the noise reduction layer is polyethers sea
It is continuous.
Compared with prior art, advantages and beneficial effects possessed by the utility model are as follows: the thallus in sample introduction cup is compacted
It is added dropwise and is delivered in sample cell under the action of dynamic pump, cooling cycle machine be delivered to again after cooling down in advance to the thallus in sample cell
In high pressure homogenizer, thallus is crushed by crushing device, while being crushed, by cooling cycle machine to high pressure homogenizer
Cool down.The utility model be delivered in high pressure homogenizer again after cooling down in advance by thallus and is crushed, can be from root
In sheet reduce thallus itself temperature, prevent because thallus self-temperature is excessively high cause downstream albumen lose biological activity,
While guaranteeing high pressure homogenizer crushing efficiency, it can also guarantee the biological activity of thallus downstream albumen itself；Simultaneously
It can reduce the energy consumption that cooling cycle machine directly cools down to high pressure homogenizer.Peristaltic pump is used for the thallus sample in sample introduction cup
Product are added dropwise in sample cell, and experimental solutions are added dropwise in substitution manual operation, avoid experiment during artificial drop takes experimental solutions
The temperature of solution can change, to influence experiment effect.Sample cell is helix tube, extends the transport path of thallus, can
Sufficiently cooled down in advance to thallus, guarantees cooling effect, and then guarantee subsequent crushing efficiency and bioactivity to thallus.Sample introduction
Thallus in slot enters in crusher chamber through feed inlet, carries out precomminution to thallus by agitating device, the thallus after smashing is through defeated
Material mouth enters in spiral conveying pipeline, while guaranteeing thallus to thallus further progress Ultrasonic Pulverization by supersonic generator
Degree of grinding and crush efficiency.The setting of spiral conveying pipeline and screw conveyor prevents thallus blocking in conveying, guarantees thallus
Normal conveying.
The thallus sample broke mechanism of the utility model is described further with reference to the accompanying drawing.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model thallus sample broke mechanism；
Fig. 2 is the structure chart of peristaltic pump in the utility model thallus sample broke mechanism；
Fig. 3 is the structure chart of crushing device in the utility model thallus sample broke mechanism；
Wherein: 1, sample introduction cup；2, hose；3, peristaltic pump；31, pump case；32, rotor；33, roll；4, cooling spiral
Pipe；5, cooling cycle machine；51, cold rinse bank；6, sample cup is received；7, high pressure homogenizer；71, body；72, sample introduction tank；73, it is crushed dress
It sets；731, crusher chamber；732, stirring motor；733, agitating shaft；734, stirring blade；735, partition；736, spiral conveying pipeline；
737, supersonic generator；738, screw conveyor.
Specific embodiment
As Figure 1-Figure 2, a kind of thallus sample broke of the utility model mechanism, including sample introduction cup, peristaltic pump, cooling follow
Ring machine, high pressure homogenizer and receipts sample cup, cooling cycle machine are equipped with cold rinse bank, are equipped with sample cell, high pressure homogenizer in cold rinse bank
Including body, body is equipped with sample introduction tank, crushing device, and sample introduction cup, sample cell, sample introduction tank, crushing device, receives sample at peristaltic pump
Cup is sequentially connected, and the outside of cold rinse bank is equipped with the first cold liquid channel, and the outside of crushing device is equipped with the second cold liquid channel, and first is cold
The liquid outlet in liquid channel is connect with the inlet in the second cold liquid channel, the inlet in the first cold liquid channel and the second cold liquid channel
Liquid outlet connection.
Sample cell is helical structure, extends the transport path of thallus, can sufficiently be cooled down in advance to thallus, guarantees cooling
Effect, and then guarantee subsequent crushing efficiency and bioactivity to thallus.
Thallus in sample introduction cup is added dropwise under the action of peristaltic pump to be delivered in sample cell, and cooling cycle machine is in sample cell
Thallus carry out in advance cool down after be delivered in high pressure homogenizer again, thallus is crushed by crushing device, be crushed while,
Cooled down by cooling cycle machine to high pressure homogenizer.The utility model carries out being delivered to high pressure again after cooling down in advance by thallus
It is crushed in homogenizer, can fundamentally reduce the temperature of thallus itself, prevent from causing because thallus self-temperature is excessively high
Albumen loses biological activity downstream, while guaranteeing high pressure homogenizer crushing efficiency, can also guarantee under thallus itself
Swim the biological activity of albumen；It also can reduce the energy consumption that cooling cycle machine directly cools down to high pressure homogenizer simultaneously.
Further, peristaltic pump include pump case, the rotor that is arranged in pump case, the end of rotor is along its circumference
Direction is uniformly equipped with roll, and the roll is positioned close on the periphery of rotor, and the top of pump case is arcuate structure, compacted
The hose of dynamic pump wears the channel formed between pump case and rotor.Hose on peristaltic pump, one end extend to sample introduction cup
Interior, the other end is connect with sample cell.Rotor rotates, the roll rotation in drive motor rotor, when the roll of rotation turns
When moving to pump case top position, rolling extrusion is carried out to the hose in channel, realizes the dropwise addition conveying of experimental solutions.Peristaltic pump is used
It is added dropwise in sample cell in by the thallus sample in sample introduction cup, experimental solutions are added dropwise in substitution manual operation, avoid taking in artificial drop
During experimental solutions, the temperature of experimental solutions can change, to influence experiment effect.
Further, as shown in figure 3, crushing device includes crusher chamber, partition is equipped in crusher chamber, partition is in back taper knot
Crusher chamber is separated into upper cavity, lower chamber by structure, partition, and upper cavity is equipped with agitating device, and agitating device includes stirring motor, stirs
Axis and stirring blade are mixed, stirring motor is fixed on the top of crusher chamber, the output end and stirring axis connection of stirring motor, paddle
The outside of agitating shaft is arranged in piece.Lower chamber is equipped with spiral conveying pipeline, supersonic generator, and the center of partition is equipped with conveying mouth,
The upper end of spiral conveying pipeline is connect with conveying mouth, and the lower end of spiral conveying pipeline is connected with screw conveyor, and supersonic generator is solid
It is scheduled on the inner wall of lower chamber, the top of crusher chamber is equipped with feed inlet, and feed inlet is connect with sample introduction tank, the discharging of screw conveyor
End is connect with sample cup is received.Thallus in sample introduction tank enters in crusher chamber through feed inlet, carries out pre- powder to thallus by agitating device
Broken, the thallus after smashing enters in spiral conveying pipeline through conveying mouth, while by supersonic generator to thallus further progress
Ultrasonic Pulverization guarantees the degree of grinding and crush efficiency of thallus.The setting of spiral conveying pipeline and screw conveyor prevents thallus from existing
It is blocked when conveying, guarantees that thallus normally conveys.
Further, the inner wall of body is equipped with noise reduction layer, and noise reduction layer is polyethers sponge, has Noise Reduction.
Embodiment described above is only that preferred embodiments of the present invention are described, not practical to this
Novel range is defined, and under the premise of not departing from the spirit of the design of the utility model, those of ordinary skill in the art are to this
The various changes and improvements that the technical solution of utility model is made should all fall into the protection that the utility model claims book determines
In range.
Claims (7)
1. a kind of thallus sample broke mechanism, it is characterised in that: including sample introduction cup, peristaltic pump, cooling cycle machine, high pressure homogenizer
With receipts sample cup, the cooling cycle machine is equipped with cold rinse bank, and sample cell is equipped in the cold rinse bank, and the sample cell is spiral knot
Structure, the high pressure homogenizer include body, and the body is equipped with sample introduction tank, crushing device, the sample introduction cup, peristaltic pump, sample
Quality control, sample introduction tank, crushing device, receipts sample cup are sequentially connected, and the outside of the cold rinse bank is equipped with the first cold liquid channel, described broken
The outside of device is equipped with the second cold liquid channel, the liquid outlet in the first cold liquid channel and the inlet in the described second cold liquid channel
Connection, the inlet in the first cold liquid channel are connect with the liquid outlet in the described second cold liquid channel.
2. thallus sample broke according to claim 1 mechanism, it is characterised in that: the peristaltic pump includes pump case, setting
The end of rotor in pump case, the rotor is uniformly equipped with roll, the roll setting along its circumferencial direction
On the periphery close to rotor, the top of the pump case is arcuate structure, and the hose of the peristaltic pump wears the pump case
The channel formed between rotor.
3. thallus sample broke according to claim 2 mechanism, it is characterised in that: the hose on the peristaltic pump, one
End extends in the sample introduction cup, and the other end is connect with the sample cell.
4. thallus sample broke according to claim 1 mechanism, it is characterised in that: the crushing device includes crusher chamber,
Partition is equipped in the crusher chamber, crusher chamber is separated into upper cavity, lower chamber by the partition, and the upper cavity is equipped with stirring dress
It sets, the lower chamber is equipped with spiral conveying pipeline, supersonic generator, and the center of the partition is equipped with conveying mouth, the spiral transferring
The upper end of expects pipe is connect with conveying mouth, and the lower end of the spiral conveying pipeline is connected with screw conveyor, the supersonic generator
It is fixed on the inner wall of lower chamber, the top of the crusher chamber is equipped with feed inlet, and the feed inlet is connect with sample introduction tank, the spiral shell
The discharge end for revolving conveyer is connect with sample cup is received.
5. thallus sample broke according to claim 4 mechanism, it is characterised in that: the partition is in inverted cone-shaped structure.
6. thallus sample broke according to claim 4 mechanism, it is characterised in that: the agitating device includes stirring electricity
Machine, agitating shaft and stirring blade, the stirring motor are fixed on the top of crusher chamber, the output end of the stirring motor and stirring
The outside of agitating shaft is arranged in axis connection, the stirring blade.
7. thallus sample broke according to claim 1 mechanism, it is characterised in that: the inner wall of the body is equipped with noise reduction
Layer, the noise reduction layer are polyethers sponge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822007880.1U CN209348806U (en) | 2018-11-30 | 2018-11-30 | Thallus sample broke mechanism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822007880.1U CN209348806U (en) | 2018-11-30 | 2018-11-30 | Thallus sample broke mechanism |
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| CN209348806U true CN209348806U (en) | 2019-09-06 |
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| CN201822007880.1U Active CN209348806U (en) | 2018-11-30 | 2018-11-30 | Thallus sample broke mechanism |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109382194A (en) * | 2018-11-30 | 2019-02-26 | 天津威特生物医药有限责任公司 | Thallus sample broke mechanism |
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2018
- 2018-11-30 CN CN201822007880.1U patent/CN209348806U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109382194A (en) * | 2018-11-30 | 2019-02-26 | 天津威特生物医药有限责任公司 | Thallus sample broke mechanism |
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