CN109382194A - Thallus sample broke mechanism - Google Patents
Thallus sample broke mechanism Download PDFInfo
- Publication number
- CN109382194A CN109382194A CN201811459617.4A CN201811459617A CN109382194A CN 109382194 A CN109382194 A CN 109382194A CN 201811459617 A CN201811459617 A CN 201811459617A CN 109382194 A CN109382194 A CN 109382194A
- Authority
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- China
- Prior art keywords
- sample
- thallus
- liquid channel
- cup
- cold
- Prior art date
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- 230000007246 mechanism Effects 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 238000001816 cooling Methods 0.000 claims abstract description 22
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims description 21
- 238000005192 partition Methods 0.000 claims description 13
- 230000009467 reduction Effects 0.000 claims description 8
- 229920000570 polyether Polymers 0.000 claims description 3
- 238000003908 quality control method Methods 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims 1
- 230000004071 biological effect Effects 0.000 abstract description 7
- 230000000694 effects Effects 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C21/00—Disintegrating plant with or without drying of the material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/0056—Other disintegrating devices or methods specially adapted for specific materials not otherwise provided for
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/18—Use of auxiliary physical effects, e.g. ultrasonics, irradiation, for disintegrating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C19/00—Other disintegrating devices or methods
- B02C19/22—Crushing mills with screw-shaped crushing means
Landscapes
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of thallus sample broke mechanisms, including sample introduction cup, peristaltic pump, cooling cycle machine, high pressure homogenizer and receipts sample cup, cooling cycle machine is equipped with cold rinse bank, sample cell is equipped in cold rinse bank, sample cell is helical structure, high pressure homogenizer includes body, body is equipped with sample introduction tank, crushing device, sample introduction cup, peristaltic pump, sample cell, sample introduction tank, crushing device, sample cup is received to be sequentially connected, the outside of cold rinse bank is equipped with the first cold liquid channel, the outside of crushing device is equipped with the second cold liquid channel, the liquid outlet in the first cold liquid channel is connect with the inlet in the second cold liquid channel, the inlet in the first cold liquid channel is connect with the liquid outlet in the second cold liquid channel.The configuration of the present invention is simple, crushing efficiency are high to can guarantee thallus downstream biological activity of albumen again simultaneously.
Description
Technical field
The present invention relates to bacterial cell disruption technical fields, more particularly to a kind of thallus sample broke mechanism.
Background technique
The inside temperature of the high-pressure homogeneous case of reduction only simple when in the prior art for BL21-FMDV-B4 bacterial cell disruption
Degree, there is no the temperature for inherently reducing BL21-FMDV-B4 thallus, therefore BL21-FMDV-B4 thallus is in shattering process
Big calorimetric is generated by high-speed impact, causes BL21-FMDV-B4 thallus downstream albumen to lose biological activity, if in order to reduce
The big calorimetric generated during BL21-FMDV-B4 bacterial cell disruption, then must reduce the cracking pressure of high pressure homogenizer, thus again
Reduce the crushing efficiency of high pressure homogenizer.
Summary of the invention
That the technical problem to be solved in the present invention is to provide a kind of structures is simple, crushing efficiency is high while can guarantee under thallus again
Swim the thallus sample broke mechanism of biological activity of albumen.
A kind of thallus sample broke mechanism of the invention, including sample introduction cup, peristaltic pump, cooling cycle machine, high pressure homogenizer and
Sample cup is received, the cooling cycle machine is equipped with cold rinse bank, and sample cell is equipped in the cold rinse bank, and the sample cell is spiral knot
Structure, the high pressure homogenizer include body, and the body is equipped with sample introduction tank, crushing device, the sample introduction cup, peristaltic pump, sample
Quality control, sample introduction tank, crushing device, receipts sample cup are sequentially connected, and the outside of the cold rinse bank is equipped with the first cold liquid channel, described broken
The outside of device is equipped with the second cold liquid channel, the liquid outlet in the first cold liquid channel and the inlet in the described second cold liquid channel
Connection, the inlet in the first cold liquid channel are connect with the liquid outlet in the described second cold liquid channel.
Preferably, the peristaltic pump include pump case, the rotor that is arranged in pump case, the end of the rotor
It is uniformly equipped with roll along its circumferencial direction, the roll is positioned close on the periphery of rotor, the top of the pump case
For arcuate structure, the hose of the peristaltic pump wears the channel formed between the pump case and rotor.
It being preferably in any of the above-described scheme, the hose on the peristaltic pump, one end extends in the sample introduction cup,
The other end is connect with the sample cell.
It is preferably in any of the above-described scheme, the crushing device includes crusher chamber, and partition, institute are equipped in the crusher chamber
It states partition and crusher chamber is separated into upper cavity, lower chamber, the upper cavity is equipped with agitating device, and the lower chamber is equipped with spiral transferring
The center of expects pipe, supersonic generator, the partition is equipped with conveying mouth, and the upper end of the spiral conveying pipeline is connect with conveying mouth,
The lower end of the spiral conveying pipeline is connected with screw conveyor, and the supersonic generator is fixed on the inner wall of lower chamber, institute
The top for stating crusher chamber is equipped with feed inlet, and the feed inlet is connect with sample introduction tank, the discharge end and receipts sample of the screw conveyor
Cup connection.
It is preferably in any of the above-described scheme, the partition is in inverted cone-shaped structure.
It is preferably in any of the above-described scheme, the agitating device includes stirring motor, agitating shaft and stirring blade, described
Stirring motor is fixed on the top of crusher chamber, the output end and stirring axis connection of the stirring motor, the stirring blade setting
In the outside of agitating shaft.
It is preferably in any of the above-described scheme, the inner wall of the body is equipped with noise reduction layer, and the noise reduction layer is polyethers sea
It is continuous.
Compared with prior art, advantage for present invention and the thallus having the beneficial effect that in sample introduction cup are in peristaltic pump
Under the action of be added dropwise and be delivered in sample cell, cooling cycle machine carries out the thallus in sample cell to be delivered to high pressure again after cooling down in advance
In homogenizer, thallus is crushed by crushing device, while being crushed, high pressure homogenizer is carried out by cooling cycle machine
Cooling.The present invention be delivered in high pressure homogenizer again after cooling down in advance by thallus and is crushed, and can fundamentally be reduced
The temperature of thallus itself, prevent because thallus self-temperature is excessively high cause downstream albumen lose biological activity, guarantee it is high
While pressing homogenizer crushing efficiency, it can also guarantee the biological activity of thallus downstream albumen itself；It also can reduce simultaneously
The energy consumption that cooling cycle machine directly cools down to high pressure homogenizer.Peristaltic pump is for the thallus sample in sample introduction cup to be added dropwise to
In sample cell, experimental solutions are added dropwise in substitution manual operation, are avoided during artificial drop takes experimental solutions, the temperature of experimental solutions
Degree can change, to influence experiment effect.Sample cell is helix tube, extends the transport path of thallus, can be sufficiently to bacterium
Body is cooled down in advance, guarantees cooling effect, and then guarantee subsequent crushing efficiency and bioactivity to thallus.Bacterium in sample introduction tank
Body enters in crusher chamber through feed inlet, carries out precomminution to thallus by agitating device, the thallus after smashing enters through conveying mouth
In spiral conveying pipeline, while by supersonic generator to thallus further progress Ultrasonic Pulverization, guarantee the degree of grinding of thallus with
And crush efficiency.The setting of spiral conveying pipeline and screw conveyor prevents thallus blocking in conveying, guarantees that thallus normally conveys.
Thallus sample broke mechanism of the invention is described further with reference to the accompanying drawing.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of thallus sample broke of the present invention mechanism；
Fig. 2 is the structure chart of peristaltic pump in thallus sample broke of the present invention mechanism；
Fig. 3 is the structure chart of crushing device in thallus sample broke of the present invention mechanism；
Wherein: 1, sample introduction cup；2, hose；3, peristaltic pump；31, pump case；32, rotor；33, roll；4, cooling spiral
Pipe；5, cooling cycle machine；51, cold rinse bank；6, sample cup is received；7, high pressure homogenizer；71, body；72, sample introduction tank；73, it is crushed dress
It sets；731, crusher chamber；732, stirring motor；733, agitating shaft；734, stirring blade；735, partition；736, spiral conveying pipeline；
737, supersonic generator；738, screw conveyor.
Specific embodiment
As Figure 1-Figure 2, a kind of thallus sample broke of the invention mechanism, including sample introduction cup, peristaltic pump, cooling cycle
Machine, high pressure homogenizer and receipts sample cup, cooling cycle machine are equipped with cold rinse bank, are equipped with sample cell, high pressure homogenizer packet in cold rinse bank
Body is included, body is equipped with sample introduction tank, crushing device, and sample introduction cup, sample cell, sample introduction tank, crushing device, receives sample cup at peristaltic pump
It is sequentially connected, the outside of cold rinse bank is equipped with the first cold liquid channel, and the outside of crushing device is equipped with the second cold liquid channel, the first cold liquid
The liquid outlet in channel is connect with the inlet in the second cold liquid channel, and the inlet in the first cold liquid channel and the second cold liquid channel go out
The connection of liquid mouth.
Sample cell is helical structure, extends the transport path of thallus, can sufficiently be cooled down in advance to thallus, guarantees cooling
Effect, and then guarantee subsequent crushing efficiency and bioactivity to thallus.
Thallus in sample introduction cup is added dropwise under the action of peristaltic pump to be delivered in sample cell, and cooling cycle machine is in sample cell
Thallus carry out in advance cool down after be delivered in high pressure homogenizer again, thallus is crushed by crushing device, be crushed while,
Cooled down by cooling cycle machine to high pressure homogenizer.The present invention be delivered to again after cooling down in advance by thallus high-pressure homogeneous
It is crushed in machine, can fundamentally reduce the temperature of thallus itself, prevent from causing under it because thallus self-temperature is excessively high
Trip albumen loses biological activity, while guaranteeing high pressure homogenizer crushing efficiency, can also guarantee thallus downstream egg itself
White biological activity；It also can reduce the energy consumption that cooling cycle machine directly cools down to high pressure homogenizer simultaneously.
Further, peristaltic pump include pump case, the rotor that is arranged in pump case, the end of rotor is along its circumference
Direction is uniformly equipped with roll, and the roll is positioned close on the periphery of rotor, and the top of pump case is arcuate structure, compacted
The hose of dynamic pump wears the channel formed between pump case and rotor.Hose on peristaltic pump, one end extend to sample introduction cup
Interior, the other end is connect with sample cell.Rotor rotates, the roll rotation in drive motor rotor, when the roll of rotation turns
When moving to pump case top position, rolling extrusion is carried out to the hose in channel, realizes the dropwise addition conveying of experimental solutions.Peristaltic pump is used
It is added dropwise in sample cell in by the thallus sample in sample introduction cup, experimental solutions are added dropwise in substitution manual operation, avoid taking in artificial drop
During experimental solutions, the temperature of experimental solutions can change, to influence experiment effect.
Further, as shown in figure 3, crushing device includes crusher chamber, partition is equipped in crusher chamber, partition is in back taper knot
Crusher chamber is separated into upper cavity, lower chamber by structure, partition, and upper cavity is equipped with agitating device, and agitating device includes stirring motor, stirs
Axis and stirring blade are mixed, stirring motor is fixed on the top of crusher chamber, the output end and stirring axis connection of stirring motor, paddle
The outside of agitating shaft is arranged in piece.Lower chamber is equipped with spiral conveying pipeline, supersonic generator, and the center of partition is equipped with conveying mouth,
The upper end of spiral conveying pipeline is connect with conveying mouth, and the lower end of spiral conveying pipeline is connected with screw conveyor, and supersonic generator is solid
It is scheduled on the inner wall of lower chamber, the top of crusher chamber is equipped with feed inlet, and feed inlet is connect with sample introduction tank, the discharging of screw conveyor
End is connect with sample cup is received.Thallus in sample introduction tank enters in crusher chamber through feed inlet, carries out pre- powder to thallus by agitating device
Broken, the thallus after smashing enters in spiral conveying pipeline through conveying mouth, while by supersonic generator to thallus further progress
Ultrasonic Pulverization guarantees the degree of grinding and crush efficiency of thallus.The setting of spiral conveying pipeline and screw conveyor prevents thallus from existing
It is blocked when conveying, guarantees that thallus normally conveys.
Further, the inner wall of body is equipped with noise reduction layer, and noise reduction layer is polyethers sponge, has Noise Reduction.
Embodiment described above only describe the preferred embodiments of the invention, not to model of the invention
It encloses and is defined, without departing from the spirit of the design of the present invention, those of ordinary skill in the art are to technical side of the invention
The various changes and improvements that case is made should all be fallen into the protection scope that claims of the present invention determines.
Claims (7)
1. a kind of thallus sample broke mechanism, it is characterised in that: including sample introduction cup, peristaltic pump, cooling cycle machine, high pressure homogenizer
With receipts sample cup, the cooling cycle machine is equipped with cold rinse bank, and sample cell is equipped in the cold rinse bank, and the sample cell is spiral knot
Structure, the high pressure homogenizer include body, and the body is equipped with sample introduction tank, crushing device, the sample introduction cup, peristaltic pump, sample
Quality control, sample introduction tank, crushing device, receipts sample cup are sequentially connected, and the outside of the cold rinse bank is equipped with the first cold liquid channel, described broken
The outside of device is equipped with the second cold liquid channel, the liquid outlet in the first cold liquid channel and the inlet in the described second cold liquid channel
Connection, the inlet in the first cold liquid channel are connect with the liquid outlet in the described second cold liquid channel.
2. thallus sample broke according to claim 1 mechanism, it is characterised in that: the peristaltic pump includes pump case, setting
The end of rotor in pump case, the rotor is uniformly equipped with roll, the roll setting along its circumferencial direction
On the periphery close to rotor, the top of the pump case is arcuate structure, and the hose of the peristaltic pump wears the pump case
The channel formed between rotor.
3. thallus sample broke according to claim 2 mechanism, it is characterised in that: the hose on the peristaltic pump, one
End extends in the sample introduction cup, and the other end is connect with the sample cell.
4. thallus sample broke according to claim 1 mechanism, it is characterised in that: the crushing device includes crusher chamber,
Partition is equipped in the crusher chamber, crusher chamber is separated into upper cavity, lower chamber by the partition, and the upper cavity is equipped with stirring dress
It sets, the lower chamber is equipped with spiral conveying pipeline, supersonic generator, and the center of the partition is equipped with conveying mouth, the spiral transferring
The upper end of expects pipe is connect with conveying mouth, and the lower end of the spiral conveying pipeline is connected with screw conveyor, the supersonic generator
It is fixed on the inner wall of lower chamber, the top of the crusher chamber is equipped with feed inlet, and the feed inlet is connect with sample introduction tank, the spiral shell
The discharge end for revolving conveyer is connect with sample cup is received.
5. thallus sample broke according to claim 4 mechanism, it is characterised in that: the partition is in inverted cone-shaped structure.
6. thallus sample broke according to claim 4 mechanism, it is characterised in that: the agitating device includes stirring electricity
Machine, agitating shaft and stirring blade, the stirring motor are fixed on the top of crusher chamber, the output end of the stirring motor and stirring
The outside of agitating shaft is arranged in axis connection, the stirring blade.
7. thallus sample broke according to claim 1 mechanism, it is characterised in that: the inner wall of the body is equipped with noise reduction
Layer, the noise reduction layer are polyethers sponge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811459617.4A CN109382194A (en) | 2018-11-30 | 2018-11-30 | Thallus sample broke mechanism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811459617.4A CN109382194A (en) | 2018-11-30 | 2018-11-30 | Thallus sample broke mechanism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109382194A true CN109382194A (en) | 2019-02-26 |
Family
ID=65430250
Family Applications (1)
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|---|---|---|---|
| CN201811459617.4A Pending CN109382194A (en) | 2018-11-30 | 2018-11-30 | Thallus sample broke mechanism |
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Application publication date: 20190226 |