CN209113907U - A kind of lactic acid dientification of bacteria detection device - Google Patents
A kind of lactic acid dientification of bacteria detection device Download PDFInfo
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- CN209113907U CN209113907U CN201821537073.4U CN201821537073U CN209113907U CN 209113907 U CN209113907 U CN 209113907U CN 201821537073 U CN201821537073 U CN 201821537073U CN 209113907 U CN209113907 U CN 209113907U
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- lactic acid
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Abstract
The utility model relates to a kind of lactic acid dientification of bacteria detection devices, including the reagent bottle for holding lactic acid bacteria sample to be detected, sampling pump, filter element, flushing pump, waste liquid pool, for holding the solvent bottle of flushing liquor, anaerobism for cultivating lactic acid bacteria sample to be detected is enriched with bottle and the detection bottle for detecting lactic acid bacteria sample to be detected, filter element includes cavity and filter core within the cavity, the both ends setting injection port and outlet of cavity, the internal cavities of filter core form filtering inner cavity, the outer wall and cavity of filter core form filtering exocoel, outer chamber wall is filtered to be open equipped with exocoel.The lactic acid dientification of bacteria detection device of the utility model saves the step of strain to be detected is moved into detector, eliminates the risk of contamination of raw material in detection process, increases detected bacterium by enrichment, keeps subsequent detection result more accurate.
Description
Technical field
The utility model belongs to identification equipment apparatus field, in particular to a kind of lactic acid dientification of bacteria detection device.
Background technique
Lactic acid bacteria is the bacterium for generating a large amount of lactic acid for fermenting raw materials with sugar, is a kind of flora beneficial to the mankind, wide
It is general applied in the dairy products eaten for the mankind.Lactic acid bacteria can be used for making Yoghourt, cheese, sour milk beverage and cultured butter;Bubble
The lactic acid bacteria contained in dish can promote intestines peristalsis, treat constipation;Soya bean and mung bean are made accordingly using lactobacillus-fermented
Drink.Lactic acid bacteria is applied in food, nutritive value of food, the flavor for improving food, the guarantor for enhancing food can be improved
Strong effect, the storage life for extending food.
It is currently that lactic acid bacteria detection method is mostly detected using instrument, lactic acid bacteria culturers is extracted into the equipment instrument that detection needs
There are many device, time-consuming very long, and pollution can be inevitably generated in sample transfer process, will also result in and seriously affect to testing result.
Utility model content
The purpose of this utility model is that in order to solve the above problem, the utility model provides a kind of lactic acid dientification of bacteria detection dress
It sets.
A kind of lactic acid dientification of bacteria detection device, including reagent bottle, the sampling pump, mistake for holding lactic acid bacteria sample to be detected
Filter unit, flushing pump, waste liquid pool, the solvent bottle for holding flushing liquor, the Anaerobic Rich for cultivating lactic acid bacteria sample to be detected
Collect bottle and the detection bottle for detecting lactic acid bacteria sample to be detected, filter element include cavity and filter core within the cavity, chamber
The both ends setting injection port and outlet of body, the internal cavities of filter core form filtering inner cavity, injection port and outlet with filtering
Inner cavity communicates, and reagent bottle is connected to injection port, and between reagent bottle and injection port, outer wall and the cavity of filter core are formed sampling pump
Exocoel is filtered, filtering outer chamber wall is open equipped with exocoel, and exocoel opening is connected to waste liquid pool and solvent bottle by triple valve, rinses
Pump is between triple valve and solvent bottle, and injection port, outlet and exocoel opening are equipped with switch valve, and anaerobism is enriched with bottle and sample introduction
It is connected between mouthful by bacterium sample delivery pipe, anaerobism enrichment bottle also has pregnant solution delivery pipe, pregnant solution delivery pipe insertion detection bottle
In, pregnant solution delivery pipe is equipped with switch valve, has lactic acid bacteria culture solution in anaerobism enrichment bottle, and detection bottle has lactic acid bacteria detection training
Nutrient solution.
Wherein, anaerobism enrichment bottle includes enrichment bottle, without oxygen gas mixture steel cylinder, pressure gauge, surge flask and vacuum pump, anaerobic
Mixed gas steel cylinder, surge flask are connected to enrichment bottle by triple valve, and surge flask is also controlled to a vacuum pump, and pressure gauge is set to nothing
The gas outlet of oxygen gas mixture steel cylinder.
Wherein, lactic acid dientification of bacteria detection device further includes enrichment water bath, and water-bath, anaerobism enrichment are equipped in enrichment water bath
Bottom of bottle portion is immersed in water-bath, and heating rod is equipped in water-bath.
Wherein, lactic acid dientification of bacteria detection device is additionally provided with the dilution flow container for having dilution, and dilution flow container passes through dilution
It is connected to inside delivery pipe and detection bottle, dilution flow container is also connected with pressurizing device, and dilution can enter detection by pressurizing device
Bottle.
The lactic acid dientification of bacteria detection device of the utility model, by lactobacillus suspension to be detected by filter element be filtered to remove it is miscellaneous
Matter obtains strain to be detected, brings in anaerobism enrichment bottle strain to be detected into using flushing liquor and is enriched with, finally enters in detection tank
Detection, the device save the step of strain to be detected is moved into detector, eliminate the risk of contamination of raw material in detection process, lead to
Crossing enrichment increases detected bacterium, keeps subsequent detection result more accurate.
Detailed description of the invention
By reading the following detailed description of the preferred embodiment, various other advantages and benefits are common for this field
Technical staff will become clear.The drawings are only for the purpose of illustrating a preferred embodiment, and is not considered as practical to this
Novel limitation.And throughout the drawings, the same reference numbers will be used to refer to the same parts.In the accompanying drawings:
Fig. 1 shows the schematic diagram of the lactic acid dientification of bacteria detection device according to the utility model embodiment.
Specific embodiment
The illustrative embodiments of the disclosure are more fully described below with reference to accompanying drawings.Although showing this public affairs in attached drawing
The illustrative embodiments opened, it being understood, however, that may be realized in various forms the disclosure without the reality that should be illustrated here
The mode of applying is limited.It is to be able to thoroughly understand the disclosure on the contrary, providing these embodiments, and can be by this public affairs
The range opened is fully disclosed to those skilled in the art.
As shown in Figure 1, a kind of lactic acid dientification of bacteria detection device, including for holding lactobacillus suspension to be detected reagent bottle 1,
Sampling pump 2, filter element 3, flushing pump 4, waste liquid pool 5, the solvent bottle 8 for holding flushing liquor, for cultivating lactic acid to be detected
The anaerobism enrichment bottle 6 of bacterium sample and the detection bottle 7 for detecting lactic acid bacteria sample to be detected, filter element 3 include cavity and position
In the intracorporal filter core of chamber, the both ends setting injection port 310 and outlet 320 of cavity, the internal cavities of filter core form filtering inner cavity,
Injection port 310 and outlet 320 are communicated with filtering inner cavity, and reagent bottle 1 is connected to injection port 310, and sampling pump 2 is located at reagent bottle
Between 1 and injection port 320, the outer wall and cavity of filter core form filtering exocoel, and filtering outer chamber wall is equipped with exocoel opening 330, outside
Chamber opening 330 is connected to waste liquid pool 5 and solvent bottle 8 by triple valve, and flushing pump 4 is between triple valve and solvent bottle 8, sample introduction
Mouth 310, outlet 320 and exocoel opening 330 are equipped with switch valve, and anaerobism is enriched with defeated by bacterium sample between bottle 6 and injection port 310
Pipe is sent to be connected to, anaerobism is enriched with bottle 6 and also has pregnant solution delivery pipe, and the insertion of pregnant solution delivery pipe detects in bottle 7, pregnant solution delivery pipe
Equipped with switch valve, there is lactic acid bacteria culture solution in anaerobism enrichment bottle, detection bottle 7 has lactic acid bacteria detection culture solution.
When detection, lactobacillus suspension to be detected is sent into injection port 310 using sampling pump 2, closing outlet 320 makes lactic acid bacteria
Impurity (metal ion, solution, small molecule compound etc.) in liquid is flowed out through the filtering of filtering inner cavity by exocoel opening 330, and
Entered in waste liquid pool 5 by triple valve.Flushing pump 4 is opened, triple valve is reversed, flushing liquor was sent by exocoel opening 330
Filter in unit 3, injection port 310 be in closed state at this time, flushing liquor with the lactic acid bacteria to be detected retained by filtering inner cavity with
Its metabolin is entered in anaerobism enrichment bottle 6 by the outflow of outlet 320 to be enriched with, and after specific time, is opened in pregnant solution delivery pipe
Switch valve, enter bacterium solution to be detected in detection bottle and detect.
It includes enrichment bottle 610, without oxygen gas mixture steel cylinder 620, pressure gauge 630, surge flask 640 and true that anaerobism, which is enriched with bottle 6,
Sky pump 650, no oxygen gas mixture steel cylinder 620, surge flask 640 be enriched with bottle 610 be connected to by triple valve, surge flask 640 also with
Vacuum pump 650 connects, and pressure gauge 630 is set to the gas outlet of no oxygen gas mixture steel cylinder 620.In use, opening vacuum pump 650
It with no oxygen gas mixture steel cylinder 620, replaces the air being enriched in bottle 610 by no oxygen gas mixture, is the richness of bacterium solution to be detected
Collection builds oxygen-free environment.
Lactic acid dientification of bacteria detection device further includes enrichment water bath 9, and water-bath is equipped in enrichment water bath 9, and anaerobism is enriched with bottle 6
Bottom is immersed in water-bath, and heating rod and temperature controller are equipped in water-bath.Heating rod and temperature controller jointly control the temperature of water-bath, make it
It is suitble to lactobacillus-fermented.
Lactic acid dientification of bacteria detection device is additionally provided with the dilution flow container 10 for having dilution, and dilution flow container is conveyed by dilution
It is connected to inside pipe and detection bottle 7, dilution flow container is also connected with pressurizing device, and dilution can enter detection bottle 7 by pressurizing device.
The preferable specific embodiment of the above, only the utility model, but the protection scope of the utility model is not
It is confined to this, anyone skilled in the art within the technical scope disclosed by the utility model, can readily occur in
Change or replacement, should be covered within the scope of the utility model.Therefore, the protection scope of the utility model should be with
Subject to the scope of protection of the claims.
Claims (4)
1. a kind of lactic acid dientification of bacteria detection device, which is characterized in that including the reagent bottle for holding lactobacillus suspension to be detected
(1), sampling pump (2), filter element (3), flushing pump (4), waste liquid pool (5), the solvent bottle (8) for holding flushing liquor, be used for
Anaerobism enrichment bottle (6) for cultivating lactic acid bacteria sample to be detected and the detection bottle (7) for detecting lactic acid bacteria sample to be detected, it is described
Filter element (3) includes cavity and filter core within the cavity, and injection port (310) and outlet is arranged in the both ends of the cavity
(320), the internal cavities of the filter core form filtering inner cavity, the injection port (310) and the outlet (320) with it is described
Filtering inner cavity communicates, and the reagent bottle (1) is connected to the injection port (310), and the sampling pump (2) is located at the reagent bottle
(1) between the injection port (310), the outer wall of the filter core and the cavity form filtering exocoel, filter and set in outer chamber wall
There is exocoel to be open (330), the exocoel opening (330) and the waste liquid pool (5) and the solvent bottle (8) are connected by triple valve
Logical, the flushing pump (4) is between the triple valve and the solvent bottle (8), the injection port (310), the outlet
(320) and exocoel opening (330) is equipped with switch valve, leads between anaerobism enrichment bottle (6) and the injection port (310)
Cross the connection of bacterium sample delivery pipe, anaerobism enrichment bottle (6) also has a pregnant solution delivery pipe, described in the pregnant solution delivery pipe insertion
It detects in bottle (7), the pregnant solution delivery pipe is equipped with switch valve, has lactic acid bacteria culture solution in the anaerobism enrichment bottle, described
Detection bottle (7) has lactic acid bacteria detection culture solution.
2. lactic acid dientification of bacteria detection device as described in claim 1, which is characterized in that
Anaerobism enrichment bottle (6) includes enrichment bottle (610), without oxygen gas mixture steel cylinder (620), pressure gauge (630), surge flask
(640) and vacuum pump (650), the no oxygen gas mixture steel cylinder (620), the surge flask (640) and the enrichment bottle (610)
It is connected to by triple valve, the surge flask (640) also connect with the vacuum pump (650), and the pressure gauge (630) is set to institute
State the gas outlet of no oxygen gas mixture steel cylinder (620).
3. lactic acid dientification of bacteria detection device as described in claim 1, which is characterized in that
The lactic acid dientification of bacteria detection device further includes enrichment water bath (9), is equipped with water-bath, institute in the enrichment water bath (9)
It states anaerobism enrichment bottle (6) bottom to immerse in water-bath, heating rod is equipped in water-bath.
4. lactic acid dientification of bacteria detection device as described in claim 1, which is characterized in that
The lactic acid dientification of bacteria detection device is additionally provided with the dilution flow container (10) for having dilution, and the dilution flow container passes through dilution
It is connected to inside liquid delivery pipe and the detection bottle (7), dilution flow container is also connected with pressurizing device, and the dilution can pass through pressurization
Device enters the detection bottle (7).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201821537073.4U CN209113907U (en) | 2018-09-19 | 2018-09-19 | A kind of lactic acid dientification of bacteria detection device |
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CN201821537073.4U CN209113907U (en) | 2018-09-19 | 2018-09-19 | A kind of lactic acid dientification of bacteria detection device |
Publications (1)
Publication Number | Publication Date |
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CN209113907U true CN209113907U (en) | 2019-07-16 |
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CN201821537073.4U Active CN209113907U (en) | 2018-09-19 | 2018-09-19 | A kind of lactic acid dientification of bacteria detection device |
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CN (1) | CN209113907U (en) |
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2018
- 2018-09-19 CN CN201821537073.4U patent/CN209113907U/en active Active
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