CN209081908U - A kind of high-precision quickly changes the cell culture apparatus of oxygen environment - Google Patents
A kind of high-precision quickly changes the cell culture apparatus of oxygen environment Download PDFInfo
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- CN209081908U CN209081908U CN201820932492.1U CN201820932492U CN209081908U CN 209081908 U CN209081908 U CN 209081908U CN 201820932492 U CN201820932492 U CN 201820932492U CN 209081908 U CN209081908 U CN 209081908U
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- insulating box
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Abstract
The utility model discloses the cell culture apparatus that a kind of high-precision quickly changes oxygen environment, belong to medicine instrument technical field.It includes insulating box (10), heater (12) and cell culture chamber (20) and singlechip controller (10), it is internal that the cell culture chamber (20) is set to insulating box (10), the nitrogen mass flow meter (32), oxygen quality flowmeter (33), periodically variable mixed gas is delivered in the humidification water bottle (40) in insulating box (10) by carbon dioxide mass flowmenter (34) under the control of singlechip controller (10) by tracheae (51), the constant temperature and humidity mixed gas of acquisition passes through brachytracheae (53) again and is delivered in cell culture chamber (20).The utility model provides the cell culture apparatus that a kind of high-precision quickly changes oxygen environment, simple for structure, can create the environment of interval anoxic, carries out the research of intermittent hypoxia cell.
Description
Technical field
The utility model relates to the cell culture apparatus that a kind of high-precision quickly changes oxygen environment, belong to medicine instrument technology
Field.
Background technique
Sleep apnea syndrome (sleep apnea syndrome, SAS), especially obstructive sleep apnea
Low hypopnea syndrome (obstructive sleep apnea-hyponea syndrome, OSAHS) is a kind of disease incidence compared with
High chronic sleep breathes illness, seriously affects the physical and mental health of people.SAS means 7h sleep every night, and apnea is repeatedly
In 30 times or more or sleep disordered breathing time index, (respiratory impair time index, RIT are put down for breaking-out
Sleep apnea number+low pass gas number per hour) >=5 times.Thus lead to the hypoxemia that repeated and high-carbon
Acidaemia can produce various influences to body: such as drowsiness, dizzy out of strength, mental act exception is had a headache from morning.With disease
Feelings progress, may occur in which the severe complications such as pulmonary hypertension, pulmonary heart disease, respiratory failure, hypertension, arrhythmia cordis.
The research of SAS is usually all built upon on the animal model of intermittent hypoxia both at home and abroad at present, not yet further into
The research of row intermittent hypoxia cell.Since cell interval anoxic is tested, gas is needed frequently to flow and exchange, meeting when gas flows
Take away the moisture content and heat in Laboratory Module, at this time keep Laboratory Module in humidity, temperature it is constant particularly important, to ensure cell energy
Therefore normal survival and growth are badly in need of researching and developing a kind of experimental provision of cell interval anoxic.
Summary of the invention
Shown on holding, the purpose of the utility model is to overcome above-mentioned deficiency, providing one kind, to can be carried out intermittent hypoxia thin
The high-precision of born of the same parents' research quickly changes the cell culture apparatus of oxygen environment.
Purpose of the utility model is realized as follows:
A kind of high-precision quickly changes the cell culture apparatus of oxygen environment comprising insulating box, heater and cell culture
Room and singlechip controller, the cell culture chamber are set to inside insulating box, and the singlechip controller is arranged in constant temperature
Outside case, the top of insulating box is arranged in the heater, is connect by the second cable with singlechip controller, in single-chip microcontroller
A degree Celsius constant temperature is provided under the control of controller for insulating box.
It further includes nitrogen mass flow meter, oxygen quality flowmeter, carbon dioxide mass flowmenter and humidification water bottle, institute
It states humidification water bottle to be set to beside the cell culture chamber in insulating box, the two is connected by brachytracheae, the nitrogen mass flow
Meter, oxygen quality flowmeter, carbon dioxide mass flowmenter are set to the outside of insulating box, pass through the first cable and single-chip microcontroller
Controller connection, and periodically variable mixed gas is delivered to insulating box by tracheae under the control of singlechip controller
In interior humidification water bottle, the constant temperature and humidity mixed gas of acquisition passes through brachytracheae again and is delivered in cell culture room；
The one side wall of the insulating box is provided with gas outlet, is overflowed the mixed gas in cell culture room by escape pipe
Insulating box.
Further, the carbon dioxide content of the periodically variable mixed gas is 5%, oxygen content in 21% and
Change between 1%.
Further, period minimum 8 seconds of the concentration variation of the periodically variable mixed gas.
Further, first cable is eight core wires.
Further, second cable is eight core wires.
The beneficial effects of the utility model are:
The utility model provides the cell culture apparatus that a kind of high-precision quickly changes oxygen environment, simple for structure, leads to
The gas of equilibrium temperature and humidity can be obtained by crossing heater and humidifying water bottle, so that cell culture indoor environment is suitble to cell raw
It is long, and changed between two states of height by oxygen content in singlechip controller periodic adjustment cell incubator and formed
Period anaerobic environment can create the environment of interval anoxic, carry out the research of intermittent hypoxia cell.
Detailed description of the invention
Fig. 1 is the schematic diagram that a kind of high-precision of the utility model quickly changes the cell culture apparatus of oxygen environment；
Wherein:
Insulating box 10
Heater 12
Gas outlet 14
Cell culture chamber 20
Nitrogen mass flow meter 32
Oxygen quality flowmeter 33
Carbon dioxide mass flowmenter 34
Humidify water bottle 40
Tracheae 51
Brachytracheae 53
Escape pipe 55
First cable 61
Second cable 63
Singlechip controller 60.
Specific embodiment
Referring to Fig. 1,Embodiment
A kind of high-precision of the utility model quickly changes the cell culture apparatus of oxygen environment, and insulating box 10 is a closed box
The top of insulating box 10 is arranged in body, heater 12, is connect by the second cable 63 with singlechip controller 60, in single-chip microcontroller
It is that insulating box 10 provides 37 degrees Celsius of constant temperature under the control of controller 60.Second cable 63 uses power supply, signal input/output one
Eight core wires of body.Cell culture chamber 20 is set in insulating box 10, can effectively keep the perseverance of temperature in cell culture chamber 20
It is fixed, to guarantee the temperature condition of culture of cell.Generally, culturing room is advisable with 200mL, and lesser volume can quickly change
The culture environment of cell.
Nitrogen mass flow meter 32, oxygen quality flowmeter 33, carbon dioxide mass flowmenter 34 are set to insulating box 10
Outside, connect with singlechip controller 60 by the first cable 61.First cable 61 is also using power supply, signal input/defeated
Eight integrated core wires out.Nitrogen mass flow meter 32, oxygen quality flowmeter 33, carbon dioxide mass flowmenter 34 are three
The concentration of gas can be carried out accurate control in real time by high-precision micrometeor gas mass flow meter.Its respectively with nitrogen
Gas source, oxygen source are connected with carbon dioxide air source, and doctor can be respectively adopted in Nitrogen source gases, oxygen source and carbon dioxide air source
It is provided with high-pure gas steel cylinder.Nitrogen mass flow meter 32, oxygen quality flow are controlled by singlechip controller 60 respectively again
The flow of meter 33, carbon dioxide mass flowmenter 34, makes the respective flow of its cyclomorphosis reach the mixed gas of various concentration.
Humidification water bottle 40 is set to 20 side of cell culture chamber in insulating box 10 nearby, and the two is connected by brachytracheae 53, brachytracheae
53 connection length is shorter as far as possible, thick, to be conducive to the quick variation of gas concentration.
The mixed gas of nitrogen, oxygen, carbon dioxide is conveyed by tracheae 51 under the control of singlechip controller 60
In humidification water bottle 40 in insulating box 10, then passes through brachytracheae 53 and be connected to cell culture chamber 20.Singlechip controller 60 weeks
Phase property adjusts oxygen content variation between two states of height in cell incubator and forms period anaerobic environment, between can creating
The environment for anoxic of having a rest carries out the research of intermittent hypoxia cell.The periodically variable mixed gas of intermittent hypoxia cell research
Carbon dioxide content is constant to be advisable 5%, oxygen content is arranged in 21% and 1%(according to different requirements) between change, concentration becomes
Change the period minimum 8 seconds, it can exploratory regulating cycle duration.The water of 40 the inside of humidification water bottle can be warming up to 37 degrees Celsius, mix
Gas is closed after humidifying water bottle, the temperature of mixed gas reaches 37 degrees Celsius, while increasing the humidity of mixed gas, obtains steady
Determine the gas of temperature and humidity.Gaseous mixture with steady temperature and humidity enters culturing room, in the control of singlechip controller 60
The lower environment that can quickly change culturing room of system, and then change cell growing environment, the more accurate simulation mould of disease
Type.Moisture is provided to cell culture chamber 20 using independent humidification water bottle 40, can effectively be kept wet in cell culture chamber 20
That spends is constant, to ensure that cell normally can survive and grow.
It is additionally provided with gas outlet 14 in the one side wall of insulating box 10, by escape pipe 55 by the mixing in cell culture chamber 20
Gas overflowing insulating box 10 makes the gaseous environment that a flowing is kept in cell culture chamber 20.
The cell culture apparatus that a kind of high-precision of the utility model quickly changes oxygen environment can be applied to simulation clinically
There is the In vitro cell model building of chronic intermittent hypoxia state in sleep apnea patient, can be adjusted according to experimental design low
Oxygen/reoxygenation time, low oxygen concentration and cycle period simulates the light middle heavy degree of chronic intermittent hypoxia in vitro well, is applicable in
In including vascular endothelial cell, the building of the chronic intermittent hypoxia damage model of smooth muscle cell etc..It even can be according to experiment
Adjustment, the hypoxic-ischemic damage that external structure duration hypoxic cell lesion mimic clinically occurs, such as myocardial infarction, cerebral infarction
Extremely, thrombotic diseases etc..Or the experimental study of anerobic glycolysis existence etc. is carried out applied to simulation tumour cell.
It should be pointed out that one skilled in the art specific embodiment of the present utility model is done it is any
Change the range of claims all without departing from the utility model.Correspondingly, the scope of the claims of the utility model
It is not limited only to previous embodiment.
Claims (4)
1. the cell culture apparatus that a kind of high-precision quickly changes oxygen environment, it is characterised in that: it includes insulating box (10), heating
Device (12) and cell culture chamber (20) and singlechip controller (60), the cell culture chamber (20) are set to insulating box (10)
Inside, singlechip controller (60) setting is external in insulating box (10), and the heater (12) is arranged in insulating box (10)
Top, connect with singlechip controller (60) by the second cable (63), be under the control of singlechip controller (60)
Insulating box (10) provides 37 degrees Celsius of constant temperature,
It further includes nitrogen mass flow meter (32), oxygen quality flowmeter (33), carbon dioxide mass flowmenter (34) and adds
Wet water bottle (40), humidification water bottle (40) are set to beside the cell culture chamber (20) in insulating box (10), and the two passes through short
Tracheae (53) connection, the nitrogen mass flow meter (32), oxygen quality flowmeter (33), carbon dioxide mass flowmenter (34)
It is set to the outside of insulating box (10), is connect by the first cable (61) with singlechip controller (60), and in single-chip microcontroller control
Periodically variable mixed gas is delivered to the humidification water in insulating box (10) by tracheae (51) under the control of device (60) processed
In bottle (40), the constant temperature and humidity mixed gas of acquisition passes through brachytracheae (53) again and is delivered in cell culture chamber (20)；
The one side wall of the insulating box (10) is provided with gas outlet (14), will be in cell culture chamber (20) by escape pipe (55)
Mixed gas overflows insulating box (10).
2. the cell culture apparatus that high-precision according to claim 1 quickly changes oxygen environment, it is characterised in that: the week
Period minimum 8 seconds of the concentration variation of the mixed gas of phase property variation.
3. the cell culture apparatus that high-precision according to claim 1 quickly changes oxygen environment, it is characterised in that: described
One cable (61) is eight core wires.
4. the cell culture apparatus that high-precision according to claim 1 quickly changes oxygen environment, it is characterised in that: described
Two cables (63) are eight core wires.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820932492.1U CN209081908U (en) | 2018-06-15 | 2018-06-15 | A kind of high-precision quickly changes the cell culture apparatus of oxygen environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820932492.1U CN209081908U (en) | 2018-06-15 | 2018-06-15 | A kind of high-precision quickly changes the cell culture apparatus of oxygen environment |
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| CN209081908U true CN209081908U (en) | 2019-07-09 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112790889A (en) * | 2021-02-01 | 2021-05-14 | 成都森威实验动物有限公司 | Preparation method and system of mixed gas for preparing intermittent hypoxia animal model |
-
2018
- 2018-06-15 CN CN201820932492.1U patent/CN209081908U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112790889A (en) * | 2021-02-01 | 2021-05-14 | 成都森威实验动物有限公司 | Preparation method and system of mixed gas for preparing intermittent hypoxia animal model |
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