CN208917200U - A kind of high extracting system device of reverse micelle protein - Google Patents
A kind of high extracting system device of reverse micelle protein Download PDFInfo
- Publication number
- CN208917200U CN208917200U CN201820920935.5U CN201820920935U CN208917200U CN 208917200 U CN208917200 U CN 208917200U CN 201820920935 U CN201820920935 U CN 201820920935U CN 208917200 U CN208917200 U CN 208917200U
- Authority
- CN
- China
- Prior art keywords
- reaction kettle
- centrifuge
- kettle
- reverse micelle
- system device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to extraction equipment field, specifically a kind of high extracting system device of reverse micelle protein.Including reaction kettle and centrifuge, the reaction kettle includes the first reaction kettle and the second reaction kettle, supersonic generator is set in first reaction kettle, pulverizer is arranged in first reaction kettle, enzyme inlet pipe and water inlet pipe are provided with below the pulverizer, first reaction kettle outlet end connects the centrifuge by pipeline, the centrifuge connects second reaction kettle, pressurizer is set in second reaction kettle, inverse micellar solution injection pipe is set above second reaction kettle, the outlet end of second reaction kettle connects another centrifuge by pipeline, the centrifuge is connected by pipeline and collects kettle.The utility model first carries out ultrasonic wave enzymolysis processing to raw material, is then acted at high temperature under high pressure by inverse micellar solution by protein extract and impurity extraction again, centrifuge is recycled to be separated, simple process, process non-environmental-pollution.
Description
Technical field
The utility model relates to extraction equipment field, specifically a kind of high extracting system device of reverse micelle protein.
Background technique
With the development of genetic and cell engineering, although traditional separation method (such as solvent extraction technology) is anti-
It is widely applied in the productions of substances such as raw element, and shows excellent separating property, but it is dfficult to apply to mentioning for protein
It takes and separates.There are two its main causes: first is that it is just unstable at 40~50 DEG C by separation object-protein etc., start to become
Property, and most protein all do not dissolve in organic solvent, if making protein and organic solvent exposure, can cause protein yet
There is denaturation；Second is that extractant problem, there are many charge, common hand ion association type extractants to be difficult to play for protein molecule surface band
Effect.Therefore research and development be easy to industrialized, efficient seriation substance separation method the people be the task of top priority.Reverse micelle extraction
Following the example of is exactly a kind of hair style is got up in this context the high extracting system device new separation technology of reverse micelle protein.
Inverse micellar solution is transparent, thermodynamically stable system.Surfactant is dissolved in nonpolar organic solvent,
When its concentration is more than critical micelle concentration (CMC), the micella formed in organic solvent is reverse micelle (reversed
) or reversed phase micelle micelle.In reverse micelle, the non-polar group of surfactant outside with nonpolar organic solvent
Contact, and polar group is then arranged in one polarity core (polar core) of interior formation.This polarity core has dissolution polar substances
Ability, after polarity karyolysis water, be formed " pond " (water pool).Inverse micelle abstraction technique has rate of extraction
Fastly, cost is relatively low, condition is of less demanding and active material will not be caused the superiority such as to lose activity, and is increasingly taken seriously,
The separating-purifying for having been widely used for the bioactive macromolecules such as protein, enzyme shows preferable application prospect.
Currently, there has been no a kind of high extracting system devices of reverse micelle protein, therefore, it is necessary to research and develop a kind of reverse micelle egg
White matter high extracting system device fills up gaps in market.
Utility model content
The utility model in order to overcome the above technical problems, provides a kind of high extracting system device of reverse micelle protein, instead
Micelle abstraction effect is good, and short the time required to extraction process, simple process, processing cost is low, and process non-environmental-pollution, extraction yield can
It sees.
To achieve the above object, the utility model uses following technical scheme:
A kind of high extracting system device of reverse micelle protein, including reaction kettle and centrifuge, the reaction kettle include first
Reaction kettle and the second reaction kettle, first reaction kettle is interior to be arranged supersonic generator, is arranged outside first reaction kettle
First controller is electrically connected the supersonic generator, is arranged feed inlet above first reaction kettle, hinged on the feed inlet
Pulverizer is arranged below the feed inlet in removable cover, be provided on the first reaction kettle wall below the pulverizer enzyme into
Pipe and water inlet pipe, first reaction kettle outlet end connect the centrifuge by pipeline, and setting pipeline connects in the centrifuge
Second reaction kettle is connect, pressurizer is set in second reaction kettle, the second control is set outside second reaction kettle
Device is electrically connected the pressurizer, and inverse micellar solution injection pipe, the outlet of second reaction kettle are arranged above second reaction kettle
End connects another centrifuge by pipeline, and the centrifuge is connected by pipeline and collects kettle.
Preferably, it is provided with blender in first reaction kettle and second reaction kettle, the blender
Driving machine is arranged in first reaction kettle and second reactor bottom, makes protein extract using the blender
It is more fully contacted with inverse micellar solution, enhances effect of extracting.
Preferably, pressure transducer is arranged in second reaction kettle, and it is electrically connected with the second controller, utilizes institute
The data that pressure transducer senses are stated, pressurizer work is controlled by the second controller, to maintain described the
Stable pressure atmosphere in two reaction kettles.
Preferably, the second reaction kettle internal upper part is provided with pressure release passage, it is provided on the pressure release passage fast
Fast pressure relief mechanism, the pressure relief mechanism include filter membrane and electrically operated valve, and the electrically operated valve is electrically connected with the second controller,
To enable second reaction kettle to reply normality as early as possible after using.
Preferably, being provided with control on the enzyme inlet pipe, the water inlet pipe and the inverse micellar solution injection pipe
Valve, the control valve respectively correspond be electrically connected first controller and the second controller, by first controller and
The second controller controls influx.
Preferably, the first reaction kettle inner wall is covered with ultrasonic wave absorbing material.
Beneficial effect is: a kind of high extracting system device of reverse micelle protein of the utility model, first carries out to raw material
Then ultrasonic wave enzymolysis processing is acted under high pressure by inverse micellar solution by protein extract and impurity extraction, then benefit again
It is separated with centrifuge, short the time required to whole process, simple process, processing cost is low, process non-environmental-pollution, extraction yield
It is considerable.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described.In all the appended drawings, similar
Element or part are generally identified by similar appended drawing reference.In attached drawing, each element or part might not be according to actual ratios
It draws.
Fig. 1 is a kind of high extracting system apparatus structure schematic diagram of reverse micelle protein of the utility model.
Appended drawing reference
Wherein 1 is the first reaction kettle, and 2 be the second reaction kettle, and 3 be centrifuge, and 4 be feed inlet, and 5 be pulverizer, 6 for enzyme into
Pipe, 7 be water inlet pipe, and 8 be the first controller, and 9 be supersonic generator, and 10 be pressurizer, and 11 be second controller, and 12 be anti-glue
Beam solution injection tube, 13 be pressure inductor, and 14 is collect kettle, and 15 be pressure release passage, and 16 be electrically operated valve, and 17 be control valve,
18 be blender, and 19 be driving machine.
Specific embodiment
The utility model is further described below with reference to embodiment.
As shown in Figure 1, a kind of high extracting system device of reverse micelle protein, including reaction kettle and centrifuge 3, the reaction
Kettle includes the first reaction kettle 1 and the second reaction kettle 2, supersonic generator 9 is arranged in first reaction kettle 1, described first
The first controller 8 is arranged outside reaction kettle 1 to be electrically connected the supersonic generator 9, feed inlet is set above first reaction kettle 1
4, hinged removable cover on the feed inlet 4 is arranged pulverizer 5 below the feed inlet 4, and described the of 5 lower section of pulverizer
Enzyme inlet pipe 6 and water inlet pipe 7 are provided on one reaction kettle, 1 wall, 1 outlet end of the first reaction kettle connects the centrifugation by pipeline
Machine 3, the interior setting pipeline of the centrifuge 3 connect second reaction kettle 2, pressurizer 10 are arranged in second reaction kettle 2,
Second controller 11 is arranged outside second reaction kettle 2 to be electrically connected the pressurizer 10, setting is anti-above second reaction kettle 2
Micellar solution injection pipe 12, the outlet end of second reaction kettle 2 connect another centrifuge 3 by pipeline, it is described from
Scheming 3 is connected by pipeline and collects kettle 14.
Preferably, being provided with blender 18, the stirring in first reaction kettle 1 and second reaction kettle 2
The driving machine 19 of device 18 is arranged in first reaction kettle 1 and 2 bottom of the second reaction kettle, is made using the blender 18
Protein extract is more fully contacted with inverse micellar solution, enhances effect of extracting.
Preferably, pressure transducer 13 is arranged in second reaction kettle 2, and it is electrically connected with the second controller 11,
The data sensed using the pressure transducer 13 are controlled the pressurizer 10 by the second controller 11 and worked,
To maintain pressure atmosphere stable in second reaction kettle 2.
Preferably, 2 internal upper part of the second reaction kettle is provided with pressure release passage 15, it is arranged on the pressure release passage 15
There is quick pressure releasing mechanism, the pressure relief mechanism includes filter membrane and electrically operated valve 16, and the electrically operated valve 16 is controlled with described second
Device 11 processed is electrically connected, to enable second reaction kettle 2 to reply normality as early as possible after using.
Preferably, being provided on the enzyme inlet pipe 6, the water inlet pipe 7 and the inverse micellar solution injection pipe 12
Control valve 17, the control valve 17 respectively correspond be electrically connected first controller 8 and the second controller 11, pass through described
One controller 8 and the second controller 11 control influx.
Preferably, 1 inner wall of the first reaction kettle is covered with ultrasonic wave absorbing material.
Through the foregoing embodiment, it can be deduced that: a kind of high extracting system device of reverse micelle protein of the utility model, it is first right
Raw material carry out ultrasonic wave enzymolysis processing, are then acted under high pressure by inverse micellar solution by protein extract and impurity again
Extraction recycles centrifuge to be separated, and short the time required to whole process, simple process, processing cost is low, and process is without environment dirt
Dye, extraction yield are considerable.
Above embodiments are only to illustrate the technical solution of the utility model, rather than its limitations；Although referring to aforementioned each
The utility model is described in detail in embodiment, those skilled in the art should understand that: it still can be right
Technical solution documented by foregoing embodiments is modified, or is equally replaced to some or all of the technical features
It changes；And these are modified or replaceed, various embodiments of the utility model technical solution that it does not separate the essence of the corresponding technical solution
Range, should all cover in the claim of the utility model and the range of specification.
Claims (6)
1. a kind of high extracting system device of reverse micelle protein, including reaction kettle and centrifuge, it is characterised in that: the reaction kettle
Including the first reaction kettle and the second reaction kettle, supersonic generator is set, in first reaction kettle in first reaction kettle
Outside the first controller of setting is electrically connected the supersonic generator, and feed inlet, the charging are arranged above first reaction kettle
Hinged removable cover on mouth, the feed inlet lower section are arranged pulverizer, set on the first reaction kettle wall below the pulverizer
It is equipped with enzyme inlet pipe and water inlet pipe, first reaction kettle outlet end connects the centrifuge by pipeline, sets in the centrifuge
It sets pipeline and connects second reaction kettle, pressurizer is set in second reaction kettle, is arranged outside second reaction kettle
Second controller is electrically connected the pressurizer, and inverse micellar solution injection pipe, second reaction are arranged above second reaction kettle
The outlet end of kettle connects another centrifuge by pipeline, and the centrifuge is connected by pipeline and collects kettle.
2. the high extracting system device of a kind of reverse micelle protein according to claim 1, it is characterised in that: described first is anti-
Answer and be provided with blender in kettle and second reaction kettle, the setting of the driving machine of the blender in first reaction kettle and
Second reactor bottom.
3. the high extracting system device of a kind of reverse micelle protein according to claim 1, it is characterised in that: described second is anti-
It answers and pressure transducer is set in kettle, and be electrically connected with the second controller.
4. the high extracting system device of a kind of reverse micelle protein according to claim 1, it is characterised in that: described second is anti-
It answers kettle internal upper part to be provided with pressure release passage, quick pressure releasing mechanism is provided on the pressure release passage, the pressure relief mechanism includes
Filter membrane and electrically operated valve, the electrically operated valve are electrically connected with the second controller.
5. the high extracting system device of a kind of reverse micelle protein according to claim 1, it is characterised in that: the enzyme into
It is provided with control valve on pipe, the water inlet pipe and the inverse micellar solution injection pipe, the control valve, which respectively corresponds, to be electrically connected
First controller and the second controller.
6. the high extracting system device of a kind of reverse micelle protein according to claim 1, it is characterised in that: described first is anti-
Kettle inner wall is answered to be covered with ultrasonic wave absorbing material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820920935.5U CN208917200U (en) | 2018-06-14 | 2018-06-14 | A kind of high extracting system device of reverse micelle protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820920935.5U CN208917200U (en) | 2018-06-14 | 2018-06-14 | A kind of high extracting system device of reverse micelle protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208917200U true CN208917200U (en) | 2019-05-31 |
Family
ID=66694765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820920935.5U Active CN208917200U (en) | 2018-06-14 | 2018-06-14 | A kind of high extracting system device of reverse micelle protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208917200U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388511A (en) * | 2021-07-02 | 2021-09-14 | 泉州市嗣家名茶发展有限公司 | Device and method for extracting plant protein from okra fruits by bundle extraction method |
| CN116440542A (en) * | 2022-06-16 | 2023-07-18 | 华大工程生物学长荡湖研究所 | Continuous flow centrifugal harvesting system and control method |
-
2018
- 2018-06-14 CN CN201820920935.5U patent/CN208917200U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388511A (en) * | 2021-07-02 | 2021-09-14 | 泉州市嗣家名茶发展有限公司 | Device and method for extracting plant protein from okra fruits by bundle extraction method |
| CN116440542A (en) * | 2022-06-16 | 2023-07-18 | 华大工程生物学长荡湖研究所 | Continuous flow centrifugal harvesting system and control method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN208917200U (en) | A kind of high extracting system device of reverse micelle protein | |
| CN101870722B (en) | Process for concentrating protein in soy protein wastewater by two-stage foam separation method | |
| CN209155177U (en) | A kind of vegetable protein extraction element | |
| CN102038793A (en) | Preparation method of soapberry saponin | |
| CN203411496U (en) | Ganoderan separation and extraction system | |
| CN102242093A (en) | Comprehensive development method of high value-added active components of eggs | |
| CN208917110U (en) | A kind of integral type reverse micelle protein extraction device | |
| CN104531342B (en) | A kind of method for extracting microbial grease | |
| CN208949307U (en) | A kind of reverse micelle protein efficient extraction plant | |
| CN202131303U (en) | Intelligent plant SOD (superoxide dismutase) extracting device | |
| CN206033640U (en) | Trichosanthes bark extraction of polysaccharide purification device | |
| CN106823458A (en) | A kind of extraction equipment | |
| KR20110040367A (en) | Method and apparatus for continuous fractionation of biomass | |
| CN110305028A (en) | A kind of device and technique of alcohol phase method washing glycine crude product | |
| CN206674838U (en) | The flat angle stratiomyiid worm material automatic separating apparatus of speck | |
| US20220204915A1 (en) | Use of a microjet reactor for processing biomass | |
| CN207811633U (en) | A kind of retort dedicated for green algae polysaccharide extraction removes slag piece-rate system | |
| CN207056016U (en) | One kind of plant protein extraction element | |
| CN110066702A (en) | A kind of dry granulation extraction process of ganoderma lucidum spore oil | |
| CN207708846U (en) | A kind of nanofiltration film condensing device producing natural pigment | |
| CN110078782A (en) | A kind of Tea Saponin extraction and purification process | |
| KR101251191B1 (en) | Method and Device for Producing Cell and Fat Solubles Material by Culturing Cell | |
| CN207002660U (en) | The reactor of wet underwater welding microbial grease | |
| CN218553186U (en) | Plant essence extractor | |
| CN205323279U (en) | Circulation ultrasound ripples draws equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |