CN207002660U - The reactor of wet underwater welding microbial grease - Google Patents

Abstract

The utility model belongs to reactor, particularly a kind of reactor of wet underwater welding microbial grease.Including open-topped tank body, the closure of tank body top surface is packaged in, the agitator being connected in tank body and with closure overhead power source, pressure gauge, thermometer and relief valve in tank body with connecting；Tank body is the jacket type structure being made up of spaced inside and outside wall, its intracavity bottom and top form loop with water-bath circulating slot by the road respectively, feed arrangement connects with charging aperture by the road, charging aperture, probe tube, reaction solution conveying end, the organic solvent connected with tank body adds mouth, lysate adds mouth and organic vapor outlet outside tank body respectively through control valve with connecting, tank wall opens up form, and probe tube is longitudinally disposed with form interval and its outlet is outside tank body.The utility model solve the problems, such as production in lack effective microbial grease extraction element, have the advantages that it is simple to operate, be easy to continuous production, production efficiency high.

Description

The reactor of wet underwater welding microbial grease

Technical field

The utility model belongs to reactor, particularly a kind of reactor of wet underwater welding microbial grease.

Background technology

In recent years, the microbial grease as energy fuel or with functions such as health cares is widely deployed and applied. The production process of microbial grease generally includes following four step：1st, it is thin that microorganism is cultivated in fermentation tank from appropriate culture medium Born of the same parents；2nd, the most liquid removed by means such as centrifugations in zymotic fluid harvest microbial cell, and are washed, are dehydrated or dried Processing；3rd, using physics (such as steam blasting, high-pressure homogenization), chemical (such as alkaline lysis), biological (such as biological enzymatic lysis) or machinery (such as grinding, squeezing) method fracturing cell walls；4th, organic solvent extraction is added into the material after broken wall or is directly centrifuged Method extraction grease.In microbial grease production procedure, grease extraction is that committed step decides the grease most in the lump Whole yield.

Compared with traditional vegetable oil extraction process, microbial cell grease extraction process has its particularity, it is impossible to directly Duplication uses vegetable fat extraction process and production equipment.The microbial cell obtained by fermented and cultured is generally present in aqueous phase In system, cell is small and oil content is high, has tough and tensile cell membrane, for the microalgae cell of most of species, Cell membrane is cellulosic structure, and some are also containing being difficult to the composition that digests, and wall thickness and strong but pliable in texture is highly detrimental to intracellular The extraction of grease, it is necessary to broken wall treatment is carried out to it in advance.

Therefore, those skilled in the art have carried out numerous studies to grease extraction, disclosed in CN102634549B The method of extraction microbial grease be that the microbial cell of collection is first dried processing, add acid solution as cracking Liquid, cell lysis wall, adds organic solvent oil and grease extracting under certain condition.CN102533879B discloses a kind of microorganism Grease extraction, it need not concentrate fermentation liquid, recovery thalline, dry the pre-treatments such as thalline operation, and directly with oil-producing Microbial fermentation mash is raw material, adds biological enzyme formulation, reacts in a mild condition with cell lysis wall, then use organic solvent Extraction obtains microbial grease.A kind of microbial oil extraction method disclosed in CN103421595A, microorganism life is prepared first Material, including the zymotic fluid of dry biomass or certain cell concentration, mixed with a certain proportion of lower alcohol-aqueous mixtures Afterwards, then using physically or chemically or the method for machinery carries out breaking-wall cell, but due to violent stirring or shake during broken wall Swing and result in emulsion so that oil and water delamination, oil phase is regathered after centrifugal treating need to being carried out.CN103421600A is carried The method for taking wet algae oil is to mix the wet algal gel of oil-producing microalgae with organic solvent, obtained mixed liquor is added to pressure-resistant anti- Answer in kettle, start to react after rising to certain temperature, reaction terminates rear blowing, collects upper strata mixing oil phase and lower floor's grain slag respectively Phase, mixing oil phase in upper strata is removed into solvent, obtains crude oil.

The common ground of above microbial oil extraction method is that the final step for extracting flow will carry out two-phase laminated flow, Grease is obtained from isolated organic phase again, however, two-phase laminated flow is relatively difficult in these grease extraction systems.Because A large amount of cell fragments are produced after microbial cell broken wall, and discharge the albumen of intracellular, nucleic acid, film fragment and with amphiphilic The material such as phosphatide, add organic solvent during extraction, mixture easily produces emulsion in the case where being stirred vigorously or shaking so that Water-oil phase is not easy to be layered, and later separation is more difficult, causes grease extraction efficiency relatively low.Therefore, many extracting methods all use The method of centrifugation is demulsified, to accelerate two-phase laminated flow.Need to extract reaction solution from reactor during centrifugation, be sent into centrifugation Machine is centrifuged, and when repeatedly extract, it is necessary to which the aqueous phase after centrifugation is re-fed into reactor, is added organic solvent, is stirred Centrifuge is re-fed into after mixing, this considerably increases operating procedure, flow is become extremely complex, and from reactor to In the transfer process of centrifuge, and insufficient loss that can cause grease of centrifugation.So two-phase laminated flow difficulty is to hinder The technical bottleneck problem of wet extraction industrial production application.

In summary, although microbial oil extraction method (especially wall-breaking method) is updated and improved, mesh It is preceding still without a kind of effective reaction unit to realize the commercial Application of these methods.

Utility model content

The purpose of this utility model is to provide a kind of special reaction device for extracting microbial grease, existing micro- to solve Lack a kind of reaction unit of effective function centralization in bio-oil extracting method to realize these method commercial Applications Problem, water-oil phase layering is difficult when specifically solving grease extraction, complex for operation step, and grease loss is serious, equipment investment The problem of larger.

Overall technology of the present utility model is conceived：

The reactor of wet underwater welding microbial grease, including open-topped tank body, it is packaged in tank body open top surface Closure, the agitator that is arranged in tank body and is connected with closure overhead power source, the pressure gauge connected with tank interior, Thermometer and relief valve；Described tank body uses the jacket type structure being made up of spaced inwall and outer wall, the jacket type The intracavity bottom of structure and top form loop by pipeline and water-bath circulating slot respectively, and feed arrangement passes through pipeline and charging aperture Connection, the charging aperture that connect with tank inner chamber, probe tube, reaction solution conveying end, organic solvent add mouth, lysate addition mouth and Organic vapor is exported respectively through control valve and tank body ft connection, and tank wall offers form, between probe tube and form Every longitudinally disposed and its outlet outside tank body.

Control valve can easily be used according to technology essential factors such as the temperature environment under its application state, roles and cut One kind in disconnected valve, regulating valve etc. or its combine to realize, because it belongs to prior art, applicant will not be repeated here.

Specific configuration of the present utility model also has：

The sealing of closure and tank body coordinates to be realized using multiple technologies scheme, real without departing from technology of the present utility model Matter, wherein preferable technical scheme is, sealing ring, tank body open top, sealing are provided between tank body open top and closure Lid and sealing ring are worn by bolt connects fixed realization sealed connection.

Effective mixing can be produced for the ease of organic phase during stirring and aqueous phase, preferable technical scheme is inner tank wall Inner surface setting have baffle plate.

Cell after Wet-process wall breaking needs to use organic solvent oil and grease extracting.Also there are some not have to organic solvent in the prior art The method of extraction, but the cell liquid centrifugation of Wet-process wall breaking is directly obtained into grease phase, but often the grease in cell can not be complete Extract entirely.Extraction efficiency can be effectively improved with organic solvent.Organic solvent easily emulsifies after being mixed with aqueous phase homogeneous Phenomenon, layering is difficult, causes organic phase to be removed so that grease can not extract.Conventional method be by mixed phase be sent into from CENTRIFUGAL ACCELERATING layering is carried out in scheming.Accelerate two phase stratification to realize reactor situ, save the equipment investment of centrifuge And mixed phase grease caused by transmit process loses, preferable technical scheme is that described inner tank wall is provided with ultrasound Generator.

For the ease of adding thalline material into reactor, preferable technical scheme is, feed arrangement include bucket body and with Its connected pusher.

For ease of the taking-up of organic phase in reactor or reaction solution, pressurization of being ventilated into tank body by pressurizeing mouth, using The inside and outside pressure difference that formed of tank body discharges liquid, and preferable technical scheme is also to include the pressurization mouth connected with tank inner chamber.

To simplify the construction of tank body, it is easy to the arrangement of pipeline, is easy to the manufacture and assembling of parts, preferable technical scheme It is that described charging aperture, probe tube, pressurization mouth, reaction solution conveying end, organic solvent add mouth, lysate adds mouth and organic Solvent vapo(u)r outlet is on closure.

Moved up and down for ease of probe tube according to the height of liquid level of organic phase and inorganic phase, preferable technical scheme is institute Outside is coordinated or is slidably matched using suit with closure in the middle part of the probe tube stated.

The slight perturbations of layering two-phase interface easily cause the fluctuation at interface, and acutely concussion can cause remixing for two-phase. Sucking-off of the fluctuation at interface to upper organic phase is very unfavorable, and the mouth of pipe readily accesses lower floor's aqueous phase so that aqueous phase is sucked out.Have Solvent should set the mouth of pipe to be located at more than interface when taking out, for the mouth of pipe that Open Side Down, in the last rank that organic solvent takes out Section, the mouth of pipe for being close to liquid level have gas suction and form perturbation wave, easily cause the sucking-off of aqueous phase.In order to accurately take out upper strata Organic phase avoids taking out lower floor aqueous phase simultaneously, and preferable technical scheme is, the arrival end of probe tube take the shape of the letter U or V-arrangement in one kind.

" entrance ", " outlet ", " inwall ", " outer wall ", " top ", " bottom ", " interior table in description of the present utility model The orientation or position relationship of the instruction such as face ", " longitudinal direction ", " outside " be based on orientation shown in the drawings or position relationship, be only for Be easy to simplify description the utility model, rather than instruction or imply signified device or element must have specific orientation, With specific azimuth configuration and operation, therefore it is not intended that to limitation of the present utility model.

The utility model so works：

The methods of collecting the zymotic fluid containing microbial cells, being dehydrated by centrifugation, flocculation sediment or film is removed in zymotic fluid Most of moisture obtains wet bacterium mud, wet bacterium mud is added in the bucket body of feed arrangement, opens the control on pusher and charging aperture Valve, wet bacterium mud are admitted in reactor, the control valve closed on charging aperture.As needed by the enzyme preparation of cell lysis wall, acid Liquid or alkali lye etc. add mouth by lysate and are added in reactor.Turn on agitator stirs lysate and bacterium mud, opens Open water-bath circulating slot to heat reactor, control suitable temperature to start cracking reaction.After reaction terminates, into reactor interlayer It is passed through cooling water temperature.Mouth is added by organic solvent and adds extractant, turn on agitator causes two-phase fully to mix extraction. Stop stirring after a period of time, open ultrasonic generator, foam and emulsion are eliminated in the presence of ultrasonic wave, reaction solution and are had Solvent accelerates layering, and a moment is stood after two phase stratification.The height of probe tube is adjusted, opens its end bend pipe by form observation The a little higher than two-phase interface layer of mouth, bottom aqueous layer is not removed after can so ensureing extraction every time, while takes out organic phase as far as possible, To reduce extraction times.By pressurizeing, mouth pressurizes, and opens the control valve of probe tube, control pressure size, has under differential pressure action Machine is mutually discharged reactor, collects organic phase.If extraction organic solvent can be added again by above-mentioned steps weight by carrying out repeatedly extraction Multiple extraction.Extraction is passed through the organic solvent evaporation that hot bath makes to remain in reactor after terminating, and finally, opens reaction solution conveying end Control valve, to reactor pressurize, the reacted liquid conveying end of bottom reaction solution is discharged in the presence of pressure difference.Filled by feeding Put and add the production that wet bacterium mud carries out next batch.

Technological progress acquired by the utility model is：

1st, the utility model is located away from integral microbial grease and extracts reaction unit for collection breaking walls and cracking and grease, avoids The multisection type operating procedure that broken wall reaction separates with grease, there is the advantages of step is simple, equipment investment is few, avoid because operating The problem of complex steps cause grease loss amount big.

2nd, the structure design of pressurization mouth, liquid can be taken out by using pressure differential method, mode of operation is easy and effective, is advantageous to Realize continuous production.

3rd, the structure design of ultrasonic generator, two phase stratification during extraction can be accelerated, extraction time is greatly shortened, carry High production efficiency.

4th, probe tube opening upwards and adjustable for height structure design, the sucking-off of lower floor's aqueous phase is effectively avoided, and can tried one's best More taking-up organic phases, improves extraction efficiency.

Brief description of the drawings

Fig. 1 is overall structure diagram of the present utility model.

Mark in accompanying drawing is as follows：

1st, pusher；2nd, pressure gauge；3rd, thermometer；4th, relief valve；5th, pressurize mouth；6th, reaction solution conveying end；7th, You Jirong Agent adds mouth；8th, lysate adds mouth；9th, organic vapor exports；10th, closure；11st, ultrasonic generator；12nd, baffle plate； 13rd, agitator；14th, form；15th, water-bath circulating slot；16th, tank body；17th, sealing ring；18th, control valve；19th, probe tube；20th, struggle against Body.

Embodiment

The utility model is further described with reference to embodiments, but not as to restriction of the present utility model, sheet The protection domain of utility model is defined by the content that claim is recorded, any equivalent technical elements made according to specification Replace, all without departing from the scope of protection of the utility model.

The overall structure of the present embodiment as shown, including open-topped tank body 16, is packaged at the top of tank body 16 and opened The closure 10 of discharge surface, the agitator 13 being connected in tank body 16 and with the overhead power source of closure 10 is arranged at, with tank body 16 Pressure gauge 2, thermometer 3 and the relief valve 4 of inside connection；Described tank body 16 is used and is made up of spaced inwall and outer wall Jacket type structure, the intracavity bottom of the jacket type structure and top form loop by pipeline and water-bath circulating slot 15 respectively, Feed arrangement is connected by pipeline with charging aperture, the charging aperture that is connected with the inner chamber of tank body 16, probe tube 19, reaction solution conveying end 6, Organic solvent adds mouth 7, lysate adds mouth 8 and organic vapor outlet 9 outside tank body 16 respectively through control valve 18 with connecting Logical, the side wall of tank body 16 offers form 14, and probe tube 19 is spaced longitudinally disposed and its outlet outside tank body 16 with form 14.

Be provided with sealing ring 17 between the open top of tank body 16 and closure 10, the open top of tank body 16, closure 10 and Sealing ring 17 is worn by bolt connects fixed realization sealed connection.

The inner surface setting of the inwall of tank body 16 has baffle plate 12.

The inwall of tank body 16 is provided with ultrasonic generator 11.

Feed arrangement includes bucket body 20 and coupled pusher 1.

Also include the pressurization mouth 5 connected with the inner chamber of tank body 16.

Charging aperture, probe tube 19, pressurization mouth 5, reaction solution conveying end 6, organic solvent add mouth 7, lysate add mouth 8 and Organic vapor outlet 9 is on closure 10.

The middle part of probe tube 19 outside is coordinated or is slidably matched using suit with closure 10.

The arrival end of described probe tube 19 take the shape of the letter U or V-arrangement in one kind.

Claims (9)

1. the reactor of wet underwater welding microbial grease, including open-topped tank body (16), it is packaged at the top of tank body (16) and opens The closure (10) of discharge surface, the agitator (13) being connected in tank body (16) and with closure (10) overhead power source is arranged at, With pressure gauge (2), thermometer (3) and the relief valve (4) connected inside tank body (16)；It is characterized in that described tank body (16) is adopted With the jacket type structure being made up of spaced inwall and outer wall, the intracavity bottom of the jacket type structure and top pass through respectively Pipeline forms loop with water-bath circulating slot (15), and feed arrangement is connected with charging aperture by pipeline, connected with tank body (16) inner chamber Charging aperture, probe tube (19), reaction solution conveying end (6), organic solvent adds mouth (7), lysate adds mouth (8) and organic molten Agent steam (vapor) outlet (9) offers form (14), taken through control valve (18) and tank body (16) ft connection, tank body (16) side wall respectively Sample pipe (19) is longitudinally disposed with form (14) interval and its outlet is located at tank body (16) outside.

2. the reactor of wet underwater welding microbial grease according to claim 1, it is characterised in that described tank body (16) Sealing ring (17), tank body (16) open top, closure (10) and sealing ring are provided between open top and closure (10) (17) worn by bolt and connect fixed realization sealed connection.

3. the reactor of wet underwater welding microbial grease according to claim 1, it is characterised in that described tank body (16) The inner surface setting of inwall has baffle plate (12).

4. the reactor of wet underwater welding microbial grease according to claim 1, it is characterised in that described tank body (16) Inwall is provided with ultrasonic generator (11).

5. the reactor of wet underwater welding microbial grease according to claim 1, it is characterised in that described feed arrangement Including bucket body (20) and coupled pusher (1).

6. the reactor of wet underwater welding microbial grease according to claim 1, it is characterised in that also include and tank body (16) the pressurization mouth (5) of inner chamber connection.

7. the reactor of the wet underwater welding microbial grease according to any one of claim 1 or 6, it is characterised in that described Charging aperture, probe tube (19), pressurization mouth (5), reaction solution conveying end (6), organic solvent add mouth (7), lysate add mouth (8) and organic vapor outlet (9) is on closure (10).

8. the reactor of wet underwater welding microbial grease according to claim 1, it is characterised in that described probe tube (19) middle part outside is coordinated or is slidably matched using suit with closure (10).

9. the reactor of the wet underwater welding microbial grease according to any one of claim 1 or 8, it is characterised in that described Probe tube (19) arrival end take the shape of the letter U or V-arrangement in one kind.