CN208917110U - A kind of integral type reverse micelle protein extraction device - Google Patents
A kind of integral type reverse micelle protein extraction device Download PDFInfo
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- CN208917110U CN208917110U CN201820920943.XU CN201820920943U CN208917110U CN 208917110 U CN208917110 U CN 208917110U CN 201820920943 U CN201820920943 U CN 201820920943U CN 208917110 U CN208917110 U CN 208917110U
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- extraction
- reaction kettle
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- reverse micelle
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Abstract
The utility model relates to extraction equipment field, specifically a kind of integral type reverse micelle protein extraction device.Including reaction kettle, feed inlet is offered above the reaction kettle, pulverizer is set below the feed inlet, it is enzymatic hydrolysis storehouse below the pulverizer, enzyme jet pipe and water spray pipe is respectively set at left and right sides of the enzymatic hydrolysis storehouse, filter screen is provided at the lower exit port in the enzymatic hydrolysis storehouse and connects extraction storehouse, the extraction storehouse side is provided with inverse micellar solution adding tube, pressurizer is arranged in the corresponding extraction bin location on the outside of the reaction kettle, discharge pipe is arranged in the extraction orlop portion, and the discharge pipe connects centrifuge.The utility model carries out raw material to crush sprinkling enzymolysis agent and water, make its Rapid contact enzyme that enzyme digestion reaction occur, the substance completed is digested through filter screen filtration, then it is acted under high pressure with inverse micellar solution again and carries out protein extraction, whole process high degree of automation, and equipment investment is reduced, one completes simple process.
Description
Technical field
The utility model relates to extraction equipment field, specifically a kind of integral type reverse micelle protein extraction device.
Background technique
With the development of genetic and cell engineering, although traditional separation method (such as solvent extraction technology) is anti-
It is widely applied in the productions of substances such as raw element, and shows excellent separating property, but it is dfficult to apply to mentioning for protein
It takes and separates.There are two its main causes: first is that it is just unstable at 40~50 DEG C by separation object-protein etc., start to become
Property, and most protein all do not dissolve in organic solvent, if making protein and organic solvent exposure, can cause protein yet
There is denaturation；Second is that extractant problem, there are many charge, common hand ion association type extractants to be difficult to play for protein molecule surface band
Effect.Therefore research and development be easy to industrialized, efficient seriation substance separation method the people be the task of top priority.Reverse micelle extraction
Following the example of is exactly a kind of hair style is got up in this context integral type reverse micelle protein extraction device new separation technology.
Inverse micellar solution is transparent, thermodynamically stable system.Surfactant is dissolved in nonpolar organic solvent,
When its concentration is more than critical micelle concentration (CMC), the micella formed in organic solvent is reverse micelle (reversed
) or reversed phase micelle micelle.In reverse micelle, the non-polar group of surfactant outside with nonpolar organic solvent
Contact, and polar group is then arranged in one polarity core (polar core) of interior formation.This polarity core has dissolution polar substances
Ability, after polarity karyolysis water, be formed " pond " (water pool).Inverse micelle abstraction technique has rate of extraction
Fastly, cost is relatively low, condition is of less demanding and active material will not be caused the superiority such as to lose activity, and is increasingly taken seriously,
The separating-purifying for having been widely used for the bioactive macromolecules such as protein, enzyme shows preferable application prospect.
Currently, there has been no a kind of integral type reverse micelle protein extraction devices, therefore, it is necessary to which it is anti-to research and develop a kind of integral type
Micella protein extraction device fills up gaps in market.
Utility model content
The utility model in order to overcome the above technical problems, provides a kind of integral type reverse micelle protein extraction device, instead
Micelle abstraction effect is good, reduces equipment investment, and one completes simple process, and processing cost is low, process non-environmental-pollution, extraction yield
It is considerable.
To achieve the above object, the utility model uses following technical scheme:
A kind of integral type reverse micelle protein extraction device, including reaction kettle, the reaction kettle top offer feed inlet,
Removable cover is installed on the feed inlet, pulverizer is set below the feed inlet, is enzymatic hydrolysis storehouse, institute below the pulverizer
It states and enzyme jet pipe and water spray pipe is respectively set at left and right sides of enzymatic hydrolysis storehouse, the spray head of the enzyme jet pipe and the water spray pipe is high pressure spray
Head, the enzymatic hydrolysis storehouse lower part is tapered, is provided with filter screen at the lower exit port in the enzymatic hydrolysis storehouse and connects feeding-passage, described
Electromagnetic valve is installed, the controller outside the reaction kettle, the charging is arranged in the electromagnetic valve connection on feeding-passage
Channel connection extraction storehouse, the extraction storehouse side are provided with inverse micellar solution adding tube, the corresponding extraction on the outside of the reaction kettle
Take bin location that pressurizer is set, discharge pipe is arranged in the extraction orlop portion, and the discharge pipe connects centrifuge.
Preferably, spiral agitator is arranged in bottom level in the reaction kettle, the driving machine of the spiral agitator is set
It sets in the reactor bottom side, makes protein extract and extracting substance more fully using the spiral agitator
Contact enhances effect of extracting.
Preferably, pressure transducer is arranged on the inner wall of the extraction storehouse, it is electrically connected with the controller, utilizes the pressure
The data that sensor sensing arrives control the pressurizer work by the controller, to maintain to stablize in the reaction kettle
Pressure atmosphere.
Preferably, pressure release passage is arranged in the extraction storehouse side, quick pressure releasing mechanism is set on the pressure release passage,
The pressure relief mechanism includes filter membrane and electrically operated valve, and the electrically operated valve is electrically connected with the controller, to finish in use
After enable the reaction kettle to reply normality as early as possible.
Preferably, on the enzyme jet pipe, the water spray pipe, the inverse micellar solution adding tube and the discharge pipe
It is provided with control valve, the control valve is electrically connected the controller, by the controller come to influx and discharge progress
Control.
Preferably, waste material discharge line, the waste material is arranged in the enzymatic hydrolysis storehouse bottom side at the filter screen
Discharge line stretches out the reaction kettle, and waste material discharge line inlet is arranged movable valve, the movable valve with it is described
Controller is electrically connected, convenient for the enzymatic hydrolysis storehouse is discharged in the substance not digested, in order to avoid block the filter screen.
Beneficial effect is: a kind of integral type reverse micelle protein extraction device of the utility model, carries out powder to raw material
Broken sprinkling enzymolysis agent and water make its Rapid contact enzyme that enzyme digestion reaction occur, digest the substance of completion through filter screen filtration, then again
It being acted under high pressure with inverse micellar solution and carries out protein extraction, the matter utilization centrifuge for finally extracting completion is separated,
Whole process high degree of automation, and equipment investment is reduced, one completes simple process, and processing cost is low, and process is without environment dirt
Dye, extraction yield are considerable.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described.In all the appended drawings, similar
Element or part are generally identified by similar appended drawing reference.In attached drawing, each element or part might not be according to actual ratios
It draws.
Fig. 1 is a kind of integral type reverse micelle protein extraction apparatus structure schematic diagram of the utility model.
Appended drawing reference
Wherein 1 is reaction kettle, and 2 be feed inlet, and 3 be pulverizer, and 4 is digest storehouse, and 5 be enzyme jet pipe, and 6 be water spray pipe, and 7 were
Strainer, 8 be electromagnetic valve, and 9 be controller, and 10 be extraction storehouse, and 11 be inverse micellar solution adding tube, and 12 be pressurizer, and 13 is out
Expect channel, 14 be centrifuge, and 15 be spiral agitator, and 16 be driving machine, and 17 be pressure inductor, and 18 be pressure release passage, and 19 are
Control valve, 20 be waste material passing away, and 21 be movable valve.
Specific embodiment
The utility model is further described below with reference to embodiment.
As shown in Figure 1, a kind of integral type reverse micelle protein extraction device, including reaction kettle 1,1 top of reaction kettle
Feed inlet 2 is offered, removable cover is installed on the feed inlet 2, pulverizer 3, the crushing are set below the feed inlet 2
It is enzymatic hydrolysis storehouse 4 below machine 3, enzyme jet pipe 5 and water spray pipe 6, the enzyme jet pipe 5 and institute is respectively set at left and right sides of the enzymatic hydrolysis storehouse 4
The spray head for stating water spray pipe 6 is high-pressure nozzle, and 4 lower parts of the enzymatic hydrolysis storehouse are tapered, is provided at the lower exit port in the enzymatic hydrolysis storehouse 4
Filter screen 7 simultaneously connects feeding-passage, and electromagnetic valve 8 is installed on the feeding-passage, and the connection of electromagnetic valve 8 is arranged described
Controller 9 outside reaction kettle 1, the feeding-passage connection extraction storehouse 10, it is molten that 10 side of extraction storehouse is provided with reverse micelle
Liquid adding tube 11 corresponds to 10 position of the extraction storehouse setting pressurizer 12 on the outside of the reaction kettle 1, and 10 bottom of extraction storehouse is set
Discharge pipe 13 is set, the discharge pipe 13 connects centrifuge 14.
Preferably, spiral agitator 15, the driving of the spiral agitator 15 is arranged in bottom level in the reaction kettle 1
Machine 16 is arranged in 1 bottom side of reaction kettle, makes protein extract and extracting substance using the spiral agitator 15
It more fully contacts, enhances effect of extracting.
Preferably, pressure transducer 17 is arranged on 10 inner wall of extraction storehouse, it is electrically connected with the controller 9, utilizes institute
The data that pressure transducer 17 senses are stated, the pressurizer 12 is controlled by the controller 9 and is worked, to remain described anti-
Answer pressure atmosphere stable in kettle 1.
Preferably, pressure release passage 18 is arranged in 10 side of extraction storehouse, it is arranged on the pressure release passage 18 and quickly lets out
Press mechanism, the pressure relief mechanism include filter membrane and electrically operated valve, and the electrically operated valve is electrically connected with the controller 9, so as to
The reaction kettle 1 is enabled to reply normality as early as possible after using.
Preferably, the enzyme jet pipe 5, the water spray pipe 6, the inverse micellar solution adding tube 11 and the discharge nozzle
It is provided with control valve 19 on road 13, the control valve 29 is electrically connected the controller 9, by the controller 9 come to influx
It is controlled with discharge.
Preferably, waste material discharge line 20 is arranged in 4 bottom side of enzymatic hydrolysis storehouse at the filter screen 7, it is described
Waste material discharge line 20 stretches out the reaction kettle 1, and movable valve 21, the activity is arranged in 20 inlet of waste material discharge line
Valve 21 is electrically connected with the controller 9, convenient for the enzymatic hydrolysis storehouse 4 is discharged in the substance not digested, in order to avoid block the filter screen
7。
Through the foregoing embodiment, it can be deduced that: a kind of integral type reverse micelle protein extraction device of the utility model, to original
Material carries out crushing sprinkling enzymolysis agent and water, makes its Rapid contact enzyme that enzyme digestion reaction occur, digests the substance of completion through filter screen
Then filtering acts under high pressure with inverse micellar solution again and carries out protein extraction, finally extract the matter utilization centrifugation of completion
Machine is separated, whole process high degree of automation, and reduces equipment investment, and one completes simple process, and processing cost is low, mistake
Journey non-environmental-pollution, extraction yield are considerable.
Above embodiments are only to illustrate the technical solution of the utility model, rather than its limitations；Although referring to aforementioned each
The utility model is described in detail in embodiment, those skilled in the art should understand that: it still can be right
Technical solution documented by foregoing embodiments is modified, or is equally replaced to some or all of the technical features
It changes；And these are modified or replaceed, various embodiments of the utility model technical solution that it does not separate the essence of the corresponding technical solution
Range, should all cover in the claim of the utility model and the range of specification.
Claims (6)
1. a kind of integral type reverse micelle protein extraction device, including reaction kettle, it is characterised in that: opened up above the reaction kettle
There is feed inlet, removable cover is installed on the feed inlet, pulverizer is set below the feed inlet, is below the pulverizer
Storehouse is digested, enzyme jet pipe and water spray pipe, the spray head of the enzyme jet pipe and the water spray pipe is respectively set at left and right sides of the enzymatic hydrolysis storehouse
For high-pressure nozzle, the enzymatic hydrolysis storehouse lower part is tapered, is provided with filter screen at the lower exit port in the enzymatic hydrolysis storehouse and connects charging
Electromagnetic valve is installed in channel on the feeding-passage, and the controller outside the reaction kettle is arranged in the electromagnetic valve connection,
Feeding-passage connection extraction storehouse, the extraction storehouse side are provided with inverse micellar solution adding tube, right on the outside of the reaction kettle
The extraction bin location setting pressurizer is answered, discharge pipe is arranged in the extraction orlop portion, and the discharge pipe connects centrifuge.
2. a kind of integral type reverse micelle protein extraction device according to claim 1, it is characterised in that: the reaction kettle
Spiral agitator is arranged in interior bottom level, and the driving machine of the spiral agitator is arranged in the reactor bottom side.
3. a kind of integral type reverse micelle protein extraction device according to claim 1, it is characterised in that: the extraction storehouse
Pressure transducer is set on inner wall, is electrically connected with the controller.
4. a kind of integral type reverse micelle protein extraction device according to claim 1, it is characterised in that: the extraction storehouse
Pressure release passage is arranged in side, quick pressure releasing mechanism is arranged on the pressure release passage, the pressure relief mechanism includes filter membrane and electricity
Movable valve, the electrically operated valve are electrically connected with the controller.
5. a kind of integral type reverse micelle protein extraction device according to claim 1, it is characterised in that: the enzyme spray
Control valve, the control valve are provided on pipe, the water spray pipe, the inverse micellar solution adding tube and the discharge pipe
Be electrically connected the controller.
6. a kind of integral type reverse micelle protein extraction device according to claim 1, it is characterised in that: the enzymatic hydrolysis storehouse
Waste material discharge line is arranged in bottom side at the filter screen, and the waste material discharge line stretches out the reaction kettle, described
Movable valve is arranged in waste material discharge line inlet, and the movable valve is electrically connected with the controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820920943.XU CN208917110U (en) | 2018-06-14 | 2018-06-14 | A kind of integral type reverse micelle protein extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820920943.XU CN208917110U (en) | 2018-06-14 | 2018-06-14 | A kind of integral type reverse micelle protein extraction device |
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| Publication Number | Publication Date |
|---|---|
| CN208917110U true CN208917110U (en) | 2019-05-31 |
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| CN201820920943.XU Active CN208917110U (en) | 2018-06-14 | 2018-06-14 | A kind of integral type reverse micelle protein extraction device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388511A (en) * | 2021-07-02 | 2021-09-14 | 泉州市嗣家名茶发展有限公司 | Device and method for extracting plant protein from okra fruits by bundle extraction method |
| CN115382247A (en) * | 2022-09-16 | 2022-11-25 | 长春师范大学 | Continuous extraction element of traditional chinese medicine |
-
2018
- 2018-06-14 CN CN201820920943.XU patent/CN208917110U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388511A (en) * | 2021-07-02 | 2021-09-14 | 泉州市嗣家名茶发展有限公司 | Device and method for extracting plant protein from okra fruits by bundle extraction method |
| CN115382247A (en) * | 2022-09-16 | 2022-11-25 | 长春师范大学 | Continuous extraction element of traditional chinese medicine |
| CN115382247B (en) * | 2022-09-16 | 2024-04-19 | 长春师范大学 | Traditional chinese medicine continuous extraction element |
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