CN208902747U - Sample detection means based on protein chip - Google Patents
Sample detection means based on protein chip Download PDFInfo
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- CN208902747U CN208902747U CN201821319533.6U CN201821319533U CN208902747U CN 208902747 U CN208902747 U CN 208902747U CN 201821319533 U CN201821319533 U CN 201821319533U CN 208902747 U CN208902747 U CN 208902747U
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Abstract
Sample detection means based on protein chip, X cross over hatch unit to board-washing guide rail；X is arranged in X to board-washing motor to board-washing guide rail one end, and X connects X to board-washing synchronous belt to board-washing motor；X is arranged in X to board-washing synchronous belt to above board-washing guide rail, and X is to the synchronous band connection synchronous belt link block of board-washing, and synchronous belt link block connects X to board-washing sliding block, and X is moved in a straight line to board-washing sliding block connection X to board-washing guide rail and along X to board-washing guide rail；Z-direction electric machine support is connected to X to board-washing upper end of slide block, Z-direction board-washing motor connects Z-direction electric machine support, Z-direction board-washing guide rail is connected to Z-direction electric machine support side, lifting seat connects Z-direction board-washing guide rail by Z-direction board-washing sliding block, hanging plate is connected to lifting seat upper end, and hanging plate extends to the top of hatch unit, and board-washing head connects hanging plate side, board-washing needle is connected to the lower end of board-washing head, and board-washing needle is distributed in the top of hatch unit.The utility model improves detection accuracy and efficiency, and performance is stablized, easy to operate.
Description
Technical field
The utility model relates to a kind of sample detection means based on protein chip, belong to protein chip detection technique neck
Domain.
Background technique
Biochip technology is a kind of high-throughput, the large-scale parallel analysis detection skill to grow up early 1990s
Art, it is using the intermolecular principle specifically to interact, by multiple technologies (such as biochemistry, micro Process, microelectronics, calculating
Machine etc.) analysis and detection technology combining together.Protein chip is a kind of high-throughout monitoring system, passes through target molecule and capture
Interaction of molecules monitors the interaction between protein molecular.Capture molecule is pre-fixed in chip surface, due to antibody
High degree of specificity and with the strong binding characteristic of antigen so by capture molecule is widely used as.Human body certain diseases (such as cancer, from
Body immunity disease etc.) it can be along with the variation of specific biomarker expression, therefore using albumen while occurring
Chip is monitored this variation, to carry out the research and diagnosis of disease.Currently, protein chip in cancer, itself exempt from
It possesses some special knowledge and applies in the diseases such as epidemic disease disease, cardiovascular disease, obesity, be especially widely used in the sieve of tumor markers
It selects and determines.
Traditional ELISA detection has certain unicity, and the project of single detection is few, and sample treatment is complicated, cost performance
Low, single sample usage amount is larger.And protein chip detection technique is once tested and can detecte several hundred kinds of target elements, it is only necessary to
The sample of 10-100UL.The processing of protein chip test sample is simple, can directly use crude biological sample (serum, blood plasma, urine,
Body fluid), can quantitative detection, cost performance is high.The existing sample detection means based on protein chip technology, structure design do not conform to
Reason, complicated for operation, sample-adding precision is low, and performance is poor, and especially concussion is incubated for and finishes, it is also necessary to which slide carrier is transferred to board-washing machine
On, detection efficiency is low.
Utility model content
The utility model in view of the deficienciess of the prior art, provide a kind of sample detection means based on protein chip,
The testing conditions that can satisfy protein chip detection technique, it is not necessary that slide carrier is transferred on board-washing machine, detection efficiency and standard
Exactness is high.
The technical solution that the utility model solves above-mentioned technical problem is as follows: the sample detection means based on protein chip,
The detection device includes work top, work top upper end be equipped with barcode scanning unit, suction nozzle unit, load bearing unit, concussion unit,
Hatch unit and board-washing unit, work top top are equipped with sample-adding unit, and the load bearing unit side is equipped with the concussion unit,
The rear side of the load bearing unit and concussion unit is equipped with the hatch unit, and the rear side of the hatch unit is equipped with the board-washing list
Member；
The board-washing unit includes board-washing pedestal, X to board-washing guide rail, X to board-washing motor, X to board-washing synchronous belt, synchronous belt
Link block, X to board-washing sliding block, Z-direction electric machine support, Z-direction board-washing motor, Z-direction board-washing guide rail, lifting seat, hanging plate, board-washing head and are washed
Plate needle；
Hanging plate, board-washing head and the board-washing needle of board-washing unit move back and forth above the hatch unit to the hatch unit
It is cleaned；
The board-washing pedestal is connected to the work top upper end, and the X is connected to the board-washing pedestal to board-washing guide rail
Upper end, X cross over the hatch unit to board-washing guide rail；The X is arranged in one end of board-washing guide rail, X to board-washing motor in the X
The X is connected to board-washing synchronous belt to board-washing motor；The X is arranged in the X to board-washing synchronous belt to above board-washing guide rail, X
To synchronous belt link block described in the synchronous band connection of board-washing, the synchronous belt link block connects the X to board-washing sliding block, the X to
Board-washing sliding block connects the X and moves in a straight line to board-washing guide rail and along X to board-washing guide rail；The Z-direction electric machine support is connected to institute
X is stated to board-washing upper end of slide block, the Z-direction board-washing motor connects the Z-direction electric machine support, and the Z-direction board-washing guide rail is connected to institute
Z-direction electric machine support side is stated, the lifting seat connects the Z-direction board-washing guide rail by Z-direction board-washing sliding block, and the hanging plate is connected to
The lifting seat upper end, hanging plate extend to the top of the hatch unit, and the board-washing head connects the hanging plate side, described to wash
Plate needle is connected to the lower end of the board-washing head, and board-washing needle is distributed in the top of the hatch unit.
As described above based on the sample detection means of protein chip, barcode scanning channel, institute are formed on the work top
Barcode scanning unit is stated equipped with bar code reader, reader fixed frame, reader attachment base, reader sliding block, reader guide rail and reading
Take device motor；The bar code reader connects the reader fixed frame, and the reader fixed frame passes through the barcode scanning channel,
Reader fixed frame drives the bar code reader along the movement outside of the load bearing unit and carries out barcode scanning；The reader connects
Joint chair connects the reader fixed frame, and the reader sliding block connects the reader attachment base；The reader guide rail connects
The reader sliding block is connect, the reader motor is connected to one end of the reader guide rail.Bar code reader belongs to existing
Technology, recognition principle are the objects due to different colours, and the wavelength of the visible light of reflection is different, and white object can reflect respectively
The visible light of kind wavelength, black object then absorb the visible light of various wavelength, so when the light of bar code scanner light source sending
After diaphragm and convex lens, when being irradiated on chequered with black and white bar code, reflected light is irradiated to light after the focusing of another convex lens
On electric transducer, then photoelectric converter receives reflected light signal of different strengths and weaknesses corresponding with informal voucher and secret note, and converts
It is output to amplification and rectification circuit at corresponding electric signal, analog signal is converted to digital electric signal by shaping circuit, then through decoding
Interface circuit is translated into numerical character information.
As described above based on the sample detection means of protein chip, the load bearing unit is equipped with sample tube and reagent
Box, the concussion unit are equipped with concussion pallet, and deep-well plates are equipped with inside concussion pallet, concussion electricity is connected with below concussion pallet
Machine, the hatch unit, which is equipped with, is incubated for pallet, is incubated for inside pallet and is equipped with slide carrier.
As described above based on the sample detection means of protein chip, the work top upper end is connected with lift frame,
The lift frame is equipped with the sample-adding unit, and the sample-adding unit is led including sample-adding pedestal, X to sample-adding crossbeam, X to sample-adding
Rail, X are loaded guide shaft, Y-direction sample-adding to sample-adding synchronous belt, X to sample-adding synchronization blocks, Y-direction sample-adding attachment base, Y-direction to sample-adding motor, X
Motor and Y-direction are loaded synchronous belt；The sample-adding pedestal is connected to the lift frame upper end, and the X is connected to sample-adding crossbeam
The sample-adding pedestal upper end, the X are connected to the X to sample-adding crossbeam upper end to sample-adding guide rail, and the X is connected to sample-adding motor
In the X to one end of sample-adding crossbeam, to X described in synchronous band connection is loaded to sample-adding motor, the X is synchronized the X to sample-adding
Block connects the X to sample-adding synchronous belt, and the Y-direction sample-adding attachment base connects the X to sample-adding synchronization blocks, and the Y-direction sample-adding is led
Y-direction described in axis connection is loaded attachment base, and Y-direction sample-adding guide shaft extends to the suction nozzle unit, load bearing unit, concussion unit and incubation
The top of unit；The Y-direction sample-adding motor is connected to the side of the Y-direction sample-adding attachment base, and Y-direction is loaded motor and connects the Y
To sample-adding synchronous belt.X to sample-adding crossbeam, X to sample-adding guide rail, X to sample-adding motor, X to sample-adding synchronous belt and X to sample-adding synchronization blocks
Design realize the X of sample-adding unit to movement, it is same that Y-direction is loaded attachment base, Y-direction sample-adding guide shaft, Y-direction sample-adding motor and Y-direction sample-adding
The Y-direction movement of sample-adding unit is realized in the design for walking band.
As described above based on the sample detection means of protein chip, the X is to being connected with X on the outside of sample-adding crossbeam to adding
Sample guide shaft, the Y-direction sample-adding attachment base are connected with auxiliary wheel mounting plate through auxiliary wheel pull rod, and auxiliary wheel mounting plate lower end connects
X is connected to cantilever auxiliary wheel, the X is contacted to sample-adding guide shaft with the X to cantilever auxiliary wheel and done straight line to sample-adding guide shaft along X
Movement.X can guarantee to be loaded accuracy of the unit X to movement to cantilever auxiliary wheel to sample-adding guide shaft and X.
As described above based on the sample detection means of protein chip, the Y-direction sample-adding guide shaft is equipped with sample-adding component, institute
Stating sample-adding component includes that sample-adding mounting base, Z-direction sample-adding guide block and Z-direction sample-adding guide rail, the sample-adding mounting base connect the Y-direction and add
Sample synchronous belt, the Z-direction sample-adding guide block connect the sample-adding mounting base, and the Z-direction sample-adding guide rail connects the Z-direction sample-adding and leads
Block.It is loaded the Z-direction movement that mounting base, Z-direction sample-adding guide block and Z-direction sample-adding guide rail realize sample-adding component.
As described above based on the sample detection means of protein chip, the Z-direction sample-adding guide rail is connected with the mobile peace in channel
Loading board, the mobile mounting plate side in the channel are connected with balance weight, and the balance weight is connected with balancing pole, connection below balance weight
There is piston motor.The design of balance weight and balancing pole can guarantee the accuracy of the Z-direction movement of piston rod.
As described above based on the sample detection means of protein chip, the balance weight side is connected with piston rod, piston
Piston/cylinder is equipped on the outside of bar, the piston motor drives the piston rod to move up and down by the balance weight, the piston
Bar lower end is equipped with air pump connector, and the air pump connector is connected with sample needle through air pump seat, is equipped with airflow channel inside air pump seat, adds
Sleeve is equipped on the outside of sample needle.Piston rod and piston/cylinder can be realized the absorption function of sample needle.
As described above based on the sample detection means of protein chip, the sample needle side is equipped with close to switch, described
The lower part of the mobile mounting plate in channel is connected to close to switch.It is a kind of without carrying out mechanically direct connect with moving component close to switch
The position switch that can operate is touched, when the sensitive surface of object proximity switch is to operating distance, Mechanical Contact is not needed and applies
Add any pressure that can make switch motion.Itself belong to the prior art close to switch.
In the utility model use process,
(1) biological sample to be detected is placed into the load bearing unit on work top, passes through the bar code reading of barcode scanning unit
Take device scanning and confirmatory sample information；
(2) it is loaded the piston motor work of unit, piston motor drives piston rod to move up and down by balance weight, and air pump connects
Head makes the suction nozzle of sample needle loading suction nozzle unit by air pump seat；
(3) sample-adding unit is moved integrally by X to sample-adding crossbeam, and it is quantitative that sample needle is moved to absorption in designated samples test tube
Then sample is assigned in the deep-well plates of concussion unit by sample；
(4) sample needle takes off suction nozzle, and the top that sample-adding unit is reset to suction nozzle unit loads suction nozzle again, draws and hold again
Reagent on carrier unit in kit is added in deep-well plates；
(5) earthquake unit mixes well reagent and sample, is then filled into the sample mixed by sample needle
In the slide carrier of hatch unit, the concussion motor of starting concussion unit carries out concussion mixing and 37 ° of incubations；
(6) after the completion of concussion is incubated for, board-washing unit motion carries out slide by board-washing needle in the top of concussion unit one by one
Carrier cleaning, takes out interpretation for slide carrier after the completion of cleaning.
The beneficial effects of the utility model are: the barcode scanning unit of the utility model, suction nozzle unit, load bearing unit, concussion list
Member, hatch unit, board-washing unit and sample-adding cell layout are reasonable, and successional sampling, reagent adding, concussion, incubation may be implemented
It being operated with board-washing etc., concussion, which is incubated for, to be finished without shifting to slide carrier, it can directly be cleaned by board-washing unit,
Improve detection efficiency；X to sample-adding crossbeam on the outside of be connected with X to sample-adding guide shaft, Y-direction sample-adding attachment base connect through auxiliary wheel pull rod
It is connected to auxiliary wheel mounting plate, auxiliary wheel mounting plate lower end is connected with X to cantilever auxiliary wheel, and X is led to cantilever auxiliary wheel and X to sample-adding
Axis contact is simultaneously moved in a straight line along X to sample-adding guide shaft, meanwhile, Z-direction sample-adding guide rail is connected with the mobile mounting plate in channel, and channel is mobile
Mounting plate side is connected with balance weight, and balance weight is connected with balancing pole, ensure that the location accuracy of sample-adding unit.
Detailed description of the invention
Fig. 1 is the sample detection means schematic perspective view based on protein chip；
Fig. 2 is the sample detection means barcode scanning cellular construction schematic diagram based on protein chip；
Fig. 3 is sample detection means suction nozzle unit, load bearing unit, concussion unit and hatch unit point based on protein chip
Cloth schematic diagram；
Fig. 4 is the sample detection means board-washing cellular construction schematic diagram based on protein chip；
Fig. 5 is that the sample detection means based on protein chip are loaded cellular construction schematic diagram；
Fig. 6 is that the sample detection means based on protein chip under another visual angle are loaded cellular construction schematic diagram；
Fig. 7 is that the sample detection means based on protein chip are loaded component structure diagram；
Fig. 8 is that the sample detection means based on protein chip are loaded component internal structural schematic diagram；
Fig. 9 is sample-adding component air pump connector schematic diagram；
Figure 10 is the sample detection methods flow chart based on protein chip；
Wherein, 1, work top；2, barcode scanning unit；3, suction nozzle unit；4, load bearing unit；5, unit is shaken；6, it is incubated for single
Member；7, board-washing unit；8, it is loaded unit；9, board-washing pedestal；10, X is to board-washing guide rail；11, X is to board-washing motor；12, X is to board-washing
Synchronous belt；13, synchronous belt link block；14, X is to board-washing sliding block；15, Z-direction electric machine support；16, Z-direction board-washing motor；17, Z-direction is washed
Panel guide；18, lifting seat；19, hanging plate；20, board-washing head；21, board-washing needle；22, barcode scanning channel；23, bar code reader；24, it reads
Take device fixed frame；25, reader attachment base；26, reader sliding block；27, reader guide rail；28, reader motor；29, sample
Test tube；30, kit；31, pallet is shaken；It is interior；32, deep-well plates；33, motor is shaken；34, it is incubated for pallet；35, slide carrier；
36, it is loaded pedestal；37, X to sample-adding crossbeam；38, X to sample-adding guide rail；39, X to sample-adding motor；40, X to sample-adding synchronous belt；41,
X to sample-adding synchronization blocks；42, Y-direction is loaded attachment base；43, Y-direction is loaded guide shaft；44, Y-direction is loaded motor；45, Y-direction sample-adding synchronizes
Band；46, lift frame；47, X to sample-adding guide shaft；48, auxiliary wheel pull rod；49；Auxiliary wheel mounting plate；50, X is to cantilever auxiliary wheel；
51, it is loaded mounting base；52, Z-direction is loaded guide block；53, Z-direction is loaded guide rail；54, the mobile mounting plate in channel；55, balance weight；56, it puts down
Weigh bar；57, piston motor；58, piston rod；59, piston/cylinder；60, air pump connector；61, sample needle；62, air pump seat；63, gas
Circulation road；64, sleeve；65, close to switch.
Specific embodiment
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, with reference to the accompanying drawing to this
The specific embodiment of utility model is described in detail.Many details are explained in the following description in order to abundant
Understand the utility model.But the utility model can be implemented with being much different from other way described herein, this field
Technical staff can do similar improvement without prejudice to the utility model connotation, therefore the utility model is not by following public affairs
The limitation for the specific embodiment opened.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" packet
Include any and all combinations of one or more related listed items.
Referring to Fig. 1 to Fig. 8, the sample detection means based on protein chip, the detection device includes work top 1, work
Make 1 upper end of table top and be equipped with barcode scanning unit 2, suction nozzle unit 3, load bearing unit 4, concussion unit 5, hatch unit 6 and board-washing unit 7,
Sample-adding unit 8 is equipped with above work top 1,4 side of load bearing unit is equipped with the concussion unit 5,4 He of load bearing unit
The rear side for shaking unit 5 is equipped with the hatch unit 6, and the rear side of the hatch unit 6 is equipped with the board-washing unit 7；
Referring to fig. 4, the board-washing unit 7 includes board-washing pedestal 9, X to board-washing guide rail 10, X to board-washing motor 11, X to washing
Plate synchronous belt 12, synchronous belt link block 13, X are led to board-washing sliding block 14, Z-direction electric machine support 15, Z-direction board-washing motor 16, Z-direction board-washing
Rail 17, lifting seat 18, hanging plate 19, board-washing head 20 and board-washing needle 21；
Hanging plate 19, board-washing head 20 and the board-washing needle 21 of board-washing unit 7 move back and forth above the hatch unit 6 to described
Hatch unit 6 is cleaned；
The board-washing pedestal 9 is connected to 1 upper end of work top, and the X is connected to the board-washing to board-washing guide rail 10
9 upper end of pedestal, X cross over the hatch unit 6 to board-washing guide rail 10；The X is arranged to board-washing motor 11 to be led in the X to board-washing
One end of rail 10, X connect the X to board-washing synchronous belt 12 to board-washing motor 11；The X is arranged to board-washing synchronous belt 12 described
X connects the synchronous belt link block 13 to board-washing synchronous belt 12 to 10 top of board-washing guide rail, X, and the synchronous belt link block 13 connects
The X is met to board-washing sliding block 14, the X connects the X to board-washing guide rail 10 to board-washing sliding block 14 and does along X to board-washing guide rail 10
Linear motion；The Z-direction electric machine support 15 is connected to the X to 14 upper end of board-washing sliding block, and the Z-direction board-washing motor 16 connects institute
Z-direction electric machine support 15 is stated, the Z-direction board-washing guide rail 17 is connected to 15 side of Z-direction electric machine support, and the lifting seat 18 passes through
Z-direction board-washing sliding block connects the Z-direction board-washing guide rail 17, and the hanging plate 19 is connected to 18 upper end of lifting seat, and hanging plate 19 extends
To the top of the hatch unit 6, the board-washing head 20 connects 19 side of hanging plate, and the board-washing needle 21 is connected to described wash
The lower end of wrench 20, board-washing needle 21 are distributed in the top of the hatch unit 6.
Referring to fig. 2, it in one embodiment of the sample detection means based on protein chip, is formed on the work top 1
There is barcode scanning channel 22, the barcode scanning unit 2 is equipped with bar code reader 23, reader fixed frame 24, reader attachment base 25, reads
Device sliding block 26, reader guide rail 27 and reader motor 28；The bar code reader 23 connects the reader fixed frame 24, institute
Reader fixed frame 24 is stated across the barcode scanning channel 22, reader fixed frame 24 drives the bar code reader 23 to hold along described
The movement outside of carrier unit 4 simultaneously carries out barcode scanning；The reader attachment base 25 connects the reader fixed frame 24, the reading
Device sliding block 26 connects the reader attachment base 25；The reader guide rail 27 connects the reader sliding block 26, the reading
Device motor 28 is connected to one end of the reader guide rail 27.
Bar code reader 23 belongs to the prior art, and recognition principle is the object due to different colours, and what is reflected is visible
The wavelength of light is different, and white object can reflect the visible light of various wavelength, and black object then absorbs the visible light of various wavelength, institute
When being irradiated on chequered with black and white bar code, to reflect when the light that bar code scanner light source issues is after diaphragm and convex lens
Light is irradiated on photoelectric converter, then photoelectric converter receives corresponding to informal voucher and secret note after the focusing of another convex lens
Reflected light signal of different strengths and weaknesses, and be converted into corresponding electric signal and be output to amplification and rectification circuit, shaping circuit is simulation
Signal is converted to digital electric signal, then is translated into numerical character information through decoding interface circuit.
Referring to Fig. 3, in one embodiment of the sample detection means based on protein chip, the load bearing unit 4 is equipped with sample
This test tube 29 and kit 30, the concussion unit 5 are equipped with concussion pallet 31, shake and are equipped with deep-well plates 32 inside pallet 31, shake
It swings and is connected with concussion motor 33 below pallet 31, the hatch unit 6, which is equipped with, is incubated for pallet 34, is incubated for inside pallet 34 and is equipped with glass
Piece carrier 35.
Referring to figs. 5 and 6, in one embodiment of the sample detection means based on protein chip, on the work top 1
End is connected with lift frame 46, and the lift frame 46 is equipped with the sample-adding unit 8, and the sample-adding unit 8 includes sample-adding bottom
Seat 36, X to sample-adding crossbeam 37, X to sample-adding guide rail 38, X to sample-adding motor 39, X to sample-adding synchronous belt 40, X to sample-adding synchronization blocks
41, Y-direction sample-adding attachment base 42, Y-direction sample-adding guide shaft 43, Y-direction sample-adding motor 44 and Y-direction are loaded synchronous belt 45；The sample-adding pedestal
36 are connected to 46 upper end of lift frame, and the X is connected to 36 upper end of sample-adding pedestal to sample-adding crossbeam 37, the X to
Sample-adding guide rail 38 is connected to the X to sample-adding 37 upper end of crossbeam, and the X is connected to the X to sample-adding crossbeam to sample-adding motor 39
37 one end, the X connect the X to sample-adding motor 39 to sample-adding synchronous belt 40, and the X connects described to sample-adding synchronization blocks 41
X connects the X to sample-adding synchronization blocks 41 to sample-adding synchronous belt 40, the Y-direction sample-adding attachment base 42, and the Y-direction is loaded guide shaft 43
The Y-direction sample-adding attachment base 42 is connected, Y-direction sample-adding guide shaft 43 extends to the suction nozzle unit 3, load bearing unit 4, concussion unit 5
With the top of hatch unit 6；The Y-direction sample-adding motor 44 is connected to the side of the Y-direction sample-adding attachment base 42, Y-direction sample-adding electricity
Machine 44 connects the Y-direction sample-adding synchronous belt 45.X is to sample-adding crossbeam 37, X to sample-adding guide rail 38, X to sample-adding motor 39, X to sample-adding
Synchronous belt 40 and X realize the X for being loaded unit 8 to the design of sample-adding synchronization blocks 41 to movement, and Y-direction is loaded attachment base 42, Y-direction sample-adding
The Y-direction movement of sample-adding unit 8 is realized in the design of guide shaft 43, Y-direction sample-adding motor 44 and Y-direction sample-adding synchronous belt 45.
In one embodiment of sample detection means based on protein chip, the X is connected to the outside of sample-adding crossbeam 37
There is X to sample-adding guide shaft 47, the Y-direction sample-adding attachment base 42 is connected with auxiliary wheel mounting plate 49 through auxiliary wheel pull rod 48, described auxiliary
Wheel 49 lower end of mounting plate is helped to be connected with X to cantilever auxiliary wheel 50, the X is to cantilever auxiliary wheel 50 and the X to sample-adding guide shaft 47
It contacts and is moved in a straight line along X to sample-adding guide shaft 47.X can guarantee that sample-adding is single to sample-adding guide shaft 47 and X to cantilever auxiliary wheel 50
Accuracy of the X of member 8 to movement.
Referring to Fig. 7, Fig. 8 and Fig. 9, in one embodiment of the sample detection means based on protein chip, the Y-direction sample-adding
Guide shaft 43 is equipped with sample-adding component, and the sample-adding component includes that sample-adding mounting base 51, Z-direction sample-adding guide block 52 and Z-direction are loaded guide rail
53, the sample-adding mounting base 51 connects the Y-direction sample-adding synchronous belt 45, and the Z-direction sample-adding guide block 52 connects the sample-adding installation
Seat 51, the Z-direction sample-adding guide rail 53 connect the Z-direction sample-adding guide block 52.Sample-adding mounting base 51, Z-direction sample-adding guide block 52 and Z-direction add
Sample guide rail 53 realizes the Z-direction movement of sample-adding component.
In one embodiment of sample detection means based on protein chip, the Z-direction sample-adding guide rail 53 is connected with channel
Mobile mounting plate 54, mobile 54 side of mounting plate in the channel are connected with balance weight 55, and the balance weight 55 is connected with balancing pole
56, piston motor 57 is connected with below balance weight 55.The design of balance weight 55 and balancing pole 56 can guarantee the Z-direction of piston rod 58
The accuracy of movement.55 side of balance weight is connected with piston rod 58, is equipped with piston/cylinder 59, the work on the outside of piston rod 58
Plug motor 57 drives the piston rod 58 to move up and down by the balance weight 55, and 58 lower end of piston rod is equipped with air pump connector
60, the air pump connector 60 is connected with sample needle 61 through air pump seat 62, is equipped with airflow channel 63, sample needle 61 inside air pump seat 62
Outside is equipped with sleeve 64.Piston rod 58 and piston/cylinder 59 can be realized the absorption function of sample needle 61.
In one embodiment of sample detection means based on protein chip, 61 side of sample needle is equipped with close to switch
65, the lower part that the mobile mounting plate 54 in channel is connected to close to switch 65.It is that one kind is not necessarily to and moving component close to switch 65
The position switch that mechanically direct contact can operate is carried out, when the sensitive surface of object proximity switch 65 is to operating distance, no
It needs Mechanical Contact and applies any pressure that switch motion can be made.Itself belong to the prior art close to switch 65.
Referring to Figure 10, the utility model relates to the sample detection methods based on protein chip the following steps are included:
S1: biological sample to be detected is placed into the load bearing unit 4 on work top 1, passes through the bar code of barcode scanning unit 2
The scanning of reader 23 and confirmatory sample information；
S2: the piston motor 57 of sample-adding unit 8 works, and piston motor 57 drives about 58 piston rod to transport by balance weight 55
Dynamic, air pump connector 60 makes the suction nozzle of the loading suction nozzle unit 3 of sample needle 61 by air pump seat 62；
S3: sample-adding unit 8 is moved integrally by X to sample-adding crossbeam 37, and sample needle 61, which is moved in designated samples test tube, to be inhaled
Quantitative sample is taken, is then assigned to sample in the deep-well plates 32 of concussion unit 5；
S4: sample needle 61 takes off suction nozzle, and the top that sample-adding unit 8 is reset to suction nozzle unit 3 loads suction nozzle again, inhales again
The reagent on load bearing unit 4 in kit 30 is taken to be added in deep-well plates 32；
S5: earthquake unit 5 mixes well reagent and sample, is then filled the sample mixed by sample needle 61
Into the slide carrier 35 of hatch unit 6, the concussion motor 33 of starting concussion unit 5 carries out concussion mixing and 37 ° of incubations；
S6: after the completion of concussion is incubated for, the movement of board-washing unit 7 is carried out in the top of concussion unit 5 by board-washing needle 21 one by one
Slide carrier 35 cleans, and slide carrier 35 is taken out interpretation after the completion of cleaning.
Barcode scanning unit 2, suction nozzle unit 3, load bearing unit 4, the concussion unit 5, hatch unit 6, board-washing list of the utility model
Member 7 and sample-adding unit 8 are rationally distributed, and the operation such as successional sampling, reagent adding, concussion, incubation and board-washing, concussion may be implemented
Incubation is finished without shifting to slide carrier 35, can directly be cleaned by board-washing unit 7, and detection effect is improved
Rate；X is connected with X to sample-adding guide shaft 47 to the outside of sample-adding crossbeam 37, and Y-direction sample-adding attachment base 42 is connected with through auxiliary wheel pull rod 48
Auxiliary wheel mounting plate 49,49 lower end of auxiliary wheel mounting plate are connected with X to cantilever auxiliary wheel 50, and X is to cantilever auxiliary wheel 50 and X to adding
The contact of sample guide shaft 47 is simultaneously moved in a straight line along X to sample-adding guide shaft 47, meanwhile, Z-direction sample-adding guide rail 53 is connected with the mobile installation in channel
Plate 54, mobile 54 side of mounting plate in channel are connected with balance weight 55, and balance weight 55 is connected with balancing pole 56, ensure that sample-adding unit
8 location accuracy.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it cannot be understood as the limitations to utility model patent range.It should be pointed out that for the common skill of this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (9)
1. the sample detection means based on protein chip, the detection device includes work top, and work top upper end is equipped with and sweeps
Code unit, suction nozzle unit, load bearing unit, concussion unit, hatch unit and board-washing unit, it is single that work top top is equipped with sample-adding
Member, it is characterised in that: the load bearing unit side is equipped with the concussion unit, sets on rear side of the load bearing unit and concussion unit
There is the hatch unit, the rear side of the hatch unit is equipped with the board-washing unit；
The board-washing unit includes board-washing pedestal, X to board-washing guide rail, X to board-washing motor, X to board-washing synchronous belt, synchronous band connection
Block, X are to board-washing sliding block, Z-direction electric machine support, Z-direction board-washing motor, Z-direction board-washing guide rail, lifting seat, hanging plate, board-washing head and board-washing
Needle；
The board-washing pedestal is connected to the work top upper end, and the X is connected to board-washing pedestal upper end to board-washing guide rail,
X crosses over the hatch unit to board-washing guide rail；To board-washing motor the X is arranged in one end of board-washing guide rail in the X, and X is to washing
Board motor connects the X to board-washing synchronous belt；The X is arranged in the X to board-washing synchronous belt to above board-washing guide rail, and X is to washing
Synchronous belt link block described in the synchronous band connection of plate, the synchronous belt link block connect the X to board-washing sliding block, and the X is to board-washing
Sliding block connects the X and moves in a straight line to board-washing guide rail and along X to board-washing guide rail；The Z-direction electric machine support be connected to the X to
Board-washing upper end of slide block, the Z-direction board-washing motor connect the Z-direction electric machine support, and the Z-direction board-washing guide rail is connected to the Z-direction
Electric machine support side, the lifting seat connect the Z-direction board-washing guide rail by Z-direction board-washing sliding block, and the hanging plate is connected to described
Lifting seat upper end, hanging plate extend to the top of the hatch unit, and the board-washing head connects the hanging plate side, the board-washing needle
It is connected to the lower end of the board-washing head, board-washing needle is distributed in the top of the hatch unit.
2. the sample detection means according to claim 1 based on protein chip, it is characterised in that: on the work top
It is formed with barcode scanning channel, it is sliding that the barcode scanning unit is equipped with bar code reader, reader fixed frame, reader attachment base, reader
Block, reader guide rail and reader motor；The bar code reader connects the reader fixed frame, the reader fixed frame
Across the barcode scanning channel, reader fixed frame drives the bar code reader along the movement outside of the load bearing unit and progress
Barcode scanning；The reader attachment base connects the reader fixed frame, and the reader sliding block connects the reader attachment base；
The reader guide rail connects the reader sliding block, and the reader motor is connected to one end of the reader guide rail.
3. the sample detection means according to claim 1 based on protein chip, it is characterised in that: the load bearing unit is set
There are sample tube and kit, the concussion unit is equipped with concussion pallet, is equipped with deep-well plates inside concussion pallet, shakes under pallet
Side is connected with concussion motor, and the hatch unit, which is equipped with, is incubated for pallet, is incubated for inside pallet and is equipped with slide carrier.
4. the sample detection means according to claim 1 based on protein chip, it is characterised in that: on the work top
End is connected with lift frame, and the lift frame is equipped with the sample-adding unit, the sample-adding unit include be loaded pedestal, X to
It is loaded crossbeam, X and is loaded attachment base, Y to sample-adding synchronous belt, X to sample-adding synchronization blocks, Y-direction to sample-adding guide rail, X to sample-adding motor, X
Synchronous belt is loaded to sample-adding guide shaft, Y-direction sample-adding motor and Y-direction；The sample-adding pedestal is connected to the lift frame upper end, described
X is connected to the sample-adding pedestal upper end to sample-adding crossbeam, and the X is connected to the X to sample-adding crossbeam upper end, institute to sample-adding guide rail
It states X and is connected to the X to one end of sample-adding crossbeam to sample-adding motor, the X synchronizes X described in band connection to sample-adding electricity to sample-adding
Machine, the X connect the X to sample-adding synchronous belt to sample-adding synchronization blocks, and it is same to sample-adding that the Y-direction sample-adding attachment base connects the X
Block is walked, the Y-direction sample-adding guide shaft connects the Y-direction and is loaded attachment base, and Y-direction sample-adding guide shaft extends to the suction nozzle unit, carrying
Unit, the top for shaking unit and hatch unit；The Y-direction sample-adding motor is connected to the side of the Y-direction sample-adding attachment base, Y
The Y-direction, which is connected, to sample-adding motor is loaded synchronous belt.
5. the sample detection means according to claim 4 based on protein chip, it is characterised in that: the X is horizontal to sample-adding
X is connected on the outside of beam to sample-adding guide shaft, the Y-direction sample-adding attachment base is connected with auxiliary wheel mounting plate, institute through auxiliary wheel pull rod
It states auxiliary wheel mounting plate lower end and is connected with X to cantilever auxiliary wheel, the X is contacted with the X to sample-adding guide shaft to cantilever auxiliary wheel
And it is moved in a straight line along X to sample-adding guide shaft.
6. the sample detection means according to claim 4 based on protein chip, it is characterised in that: the Y-direction sample-adding is led
Axis is equipped with sample-adding component, and the sample-adding component includes that sample-adding mounting base, Z-direction sample-adding guide block and Z-direction are loaded guide rail, the sample-adding
Mounting base connects the Y-direction and is loaded synchronous belt, and the Z-direction sample-adding guide block connects the sample-adding mounting base, and the Z-direction is loaded guide rail
Connect the Z-direction sample-adding guide block.
7. the sample detection means according to claim 6 based on protein chip, it is characterised in that: the Z-direction sample-adding is led
Rail is connected with the mobile mounting plate in channel, and the mobile mounting plate side in the channel is connected with balance weight, and the balance weight is connected with flat
Weigh bar, is connected with piston motor below balance weight.
8. the sample detection means according to claim 7 based on protein chip, it is characterised in that: the balance weight side
It is connected with piston rod, piston/cylinder is equipped on the outside of piston rod, the piston motor drives the piston rod by the balance weight
It moves up and down, the piston-rod lower end is equipped with air pump connector, and the air pump connector is connected with sample needle through air pump seat, in air pump seat
Portion is equipped with airflow channel, is equipped with sleeve on the outside of sample needle.
9. the sample detection means according to claim 8 based on protein chip, it is characterised in that: the sample needle side
Equipped with close to switch, the close switch is connected to the lower part of the mobile mounting plate in channel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821319533.6U CN208902747U (en) | 2018-08-15 | 2018-08-15 | Sample detection means based on protein chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821319533.6U CN208902747U (en) | 2018-08-15 | 2018-08-15 | Sample detection means based on protein chip |
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| Publication Number | Publication Date |
|---|---|
| CN208902747U true CN208902747U (en) | 2019-05-24 |
Family
ID=66569366
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821319533.6U Active CN208902747U (en) | 2018-08-15 | 2018-08-15 | Sample detection means based on protein chip |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108957022A (en) * | 2018-08-15 | 2018-12-07 | 烟台艾德康生物科技有限公司 | Sample detection means and method based on protein chip |
-
2018
- 2018-08-15 CN CN201821319533.6U patent/CN208902747U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108957022A (en) * | 2018-08-15 | 2018-12-07 | 烟台艾德康生物科技有限公司 | Sample detection means and method based on protein chip |
| CN108957022B (en) * | 2018-08-15 | 2023-12-26 | 烟台艾德康生物科技有限公司 | Sample detection device and method based on protein chip |
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