CN208883882U - Loading device and gene sequencing system - Google Patents
Loading device and gene sequencing system Download PDFInfo
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- CN208883882U CN208883882U CN201821327073.1U CN201821327073U CN208883882U CN 208883882 U CN208883882 U CN 208883882U CN 201821327073 U CN201821327073 U CN 201821327073U CN 208883882 U CN208883882 U CN 208883882U
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Abstract
The utility model provides a kind of loading device, biochemical reaction is carried out on sequence testing chip for loading on different liquids reactant, including cover board and pedestal, seal groove and flow path groove are offered on the cover board bottom surface, the flow path groove is set to the inside of the seal groove, an and at least c-type structure, the cover board is contacted on the sequence testing chip by the sealing element of the insertion seal groove and forms cavity, the flow path groove is equipped with inlet and liquid outlet, the inlet or the liquid outlet cross over the seal groove by the correspondence runner inside the cover board, and it is connected to the solenoid valve on the pedestal, the solenoid valve is for controlling liquid feeding and drain.The application of the device is not so that sequence testing chip needs expensive encapsulation process, and reactant can be loaded directly on naked sequence testing chip, loading procedure bubble-free, and it is steady to change liquid, and fringe region changes that liquid is more abundant, is conducive to the automatic biological reaction of quick, big flux.The utility model also provides a kind of gene sequencing system.
Description
Technical field
The utility model relates to gene technology fields, particularly relate to a kind of for several liquid reactants to be loaded on naked survey
The loading device of biochemical reaction, loading method, and the gene sequencing system using the device are carried out on sequence chip.
Background technique
This part intends to provides background or context for the embodiments of the present invention stated in claims.This
The description at place recognizes it is the prior art not because not being included in this section.
Gene sequencing chip (such as silicon wafer) needs and a variety of different reagents carry out biological respinse and can fix on its surface
Or gene molecule or nucleic acid molecules are carried, liquid speed degree is changed, liquid measure is changed, changes the liquid time, change liquid temperature, change liquid during load reagent
Stationarity requires strict control, and chip is unable to ingress of air during replacing reagent, so chip loads reaction process
It needs to carry out inside the locking device being especially tailored.By taking the load of gene nano sphere molecule (DNB) as an example, prior art
It is a kind of closed DNB loading method of silicon wafer, each chip is carried out special processing in advance and encapsulates (chip front side first by this method
Fixed position lid sets sheet glass, and edge glue dispensing and packaging makes internal formation runner, and wherein runner both ends, which correspond to, opens up feed liquor on chip
Mouth and liquid outlet, and combined chip-glass piece is fixed using frame), DNB or reagent are connected to feed liquor by the chip after encapsulation
Mouthful, the syringe drawing liquid through liquid outlet generates negative pressure, and reactant carries out biological respinse from inlet suction passage and after being full of, after
Reactant is until the reaction is complete in continuous drawing liquid replacement runner.
The encapsulation process of above-mentioned silicon chip DNB loading method, each chip is complicated, and the precision of runner, leakproofness are wanted
Ask high, the scrappage of chip is high when encapsulation；Auxiliary material (sheet glass, frame) demand is big simultaneously, at high cost；Moreover, often
One chip is both needed to encapsulation process, is unfavorable for flexibly using, and especially chip is difficult to exclude after air inlet bubble, and packaged glass piece
After be unfavorable for carrying out quickly, big throughput automated biological respinse.
Utility model content
In view of the foregoing, it is necessary to provide a kind of improved for several liquid reactants to be loaded on naked sequence testing chip
On loading device, loading method and gene sequencing system, quick, big throughput automated biological respinse can be carried out.
Technical solution provided by the utility model are as follows: a kind of loading device, for liquid reactant to be loaded on sequencing core
On piece carries out biochemical reaction, including cover board and pedestal, offers seal groove and flow path groove, the flow path groove on the cover board bottom surface
Set on the inside of the seal groove, and an at least c-type structure, the cover board are contradicted by being embedded in the sealing element of the seal groove
In on the sequence testing chip and forming cavity, the flow path groove is equipped with inlet and liquid outlet, the inlet or it is described go out liquid
Mouth crosses over the seal groove by the correspondence runner inside the cover board, and is connected to the solenoid valve on the pedestal, the electricity
Magnet valve is for controlling liquid feeding and drain.
Further, the cavity is rectangular.
Further, the flow path groove is equipped with a straight trough and the C slot with its face, and the flow path groove is equipped with several feed liquors
The interface of mouth and liquid outlet, the inlet or the liquid outlet and the pedestal is equipped with second seal.
Specifically, the end of the straight trough is respectively equipped with an inlet and a liquid outlet.
Specifically, the corner of the C slot is respectively equipped with a liquid outlet.
Further, the interface of the inlet or the liquid outlet and the pedestal is equipped with second seal.
Further, the second seal is embedded in inside the cover board, and is contacted on the pedestal.
Further, the solenoid valve includes the liquid feed valve, tapping valve and an exhaust valve of corresponding number, wherein the feed liquor
Valve is connected conducting into liquid feeding flow path with pipeline with infusion port, the inlet, and each tapping valve goes out with described in corresponding one
Liquid mouth and waste liquid port are connected conducting liquid stream road in a row with pipeline, the exhaust valve and the infusion port, the liquid feed valve, extremely
A few tapping valve and the waste liquid port are connected with pipeline to be connected into exhaust flow path.
Specifically, the solenoid valve is equipped with 5, including 1 liquid feed valve, 1 exhaust valve and 3 tapping valves, wherein described
Liquid feed valve and infusion port, the inlet are connected conducting into liquid feeding flow path with pipeline, each tapping valve and a corresponding institute
Liquid outlet and waste liquid port are stated with the connected conducting liquid stream road in a row of pipeline, the exhaust valve and the infusion port, the feed liquor
Valve, at least one tapping valve and the waste liquid port are connected conducting into exhaust flow path with pipeline.
Further, the pedestal is successively arranged the first heat-conducting plate, valve seat and the second heat-conducting plate, the valve seat from top to bottom
For hollow structure, the bottom of first heat-conducting plate is contacted with second heat-conducting plate and fixed, the side of first heat-conducting plate
Side upper surface is contacted with the cover board and screw is fixed, and lower surface is contacted with the valve seat.
Further, the top surface of first heat-conducting plate is equipped with the groove outside the conducting of side, sets the sequencing for card
Chip, and the sequence testing chip top surface is concordant with the side upper surface of first heat-conducting plate after installation.
Further, the recess outlet of first heat-conducting plate is used for the stretching of the sequence testing chip, and extension sets up separately
There is clamping piece.
Further, described by the fixed depth adjustment to realize cavity of screw between the cover board and the pedestal
Cover board is equipped with positioning through hole.
The utility model further provides for a kind of gene sequencing system, impregnates reaction test system, packet for naked sequence testing chip
Include fluid system, heating system and the loading device, the infusion port company of the fluid system and the loading device
Logical, and the opening and closing of solenoid valve described in automatic program control, the second of the heating system and the loading device is thermally conductive
The bottom of plate contacts to provide and control the required temperature of reaction.
Compared with prior art, loading device provided by the utility model, for liquid reactant to be loaded on sequencing core
On piece carries out biochemical reaction, including cover board and pedestal, offers seal groove and flow path groove, the flow path groove on the cover board bottom surface
Set on the inside of the seal groove, and an at least c-type structure, the cover board are contradicted by being embedded in the sealing element of the seal groove
In on the sequence testing chip and forming cavity, the flow path groove is equipped with inlet and liquid outlet, the inlet or it is described go out liquid
Mouth crosses over the seal groove by the correspondence runner inside the cover board, and is connected to the solenoid valve on the pedestal, the electricity
Magnet valve is for controlling liquid feeding and drain.So that sequence testing chip does not need expensive encapsulation process, reactant can for the application of the device
It is loaded directly on naked sequence testing chip, at low cost, it is convenient to operate；Its loading method, simply, efficiently, loading procedure bubble-free is changed
Liquid is steadily abundant, is conducive to the automatic biological reaction of quick, big flux；It can automatically will be different using its gene sequencing system
Reactant is loaded on naked sequence testing chip, so that it is carried gene molecule or nucleic acid molecules, is not only increased the utilization of sequence testing chip
Rate, sequencing throughput are big；And the needs of repeated authentication test can be realized in single, and testing efficiency is high, and test result is more acurrate；Together
When automation loading procedure control, in conjunction with seal cavity design so that the systematic error of repeated loading is small, test result more may be used
It leans on.
Detailed description of the invention
Utility model will be further described in detail below with reference to the attached drawings and specific embodiments.
Fig. 1 is the perspective view of the loading device of one embodiment of the utility model.
Fig. 2 is the exploded view that loading device shown in FIG. 1 is presented from a visual angle.
Fig. 3 is the exploded view that loading device shown in FIG. 1 is presented from another visual angle.
Fig. 4 is longitudinal section view of the cover board shown in FIG. 1 at inlet.
Fig. 5 is the bottom view of cover board shown in FIG. 1.
Description of symbols:
Loading device 100
Cover board 10
Sealing element 11
Second seal 12
Positioning through hole 13
Tapped through hole 14
Seal groove 15
Flow path groove 16
Inlet 161
First liquid outlet 162
Second liquid outlet 163
Third liquid outlet 164
First heat-conducting plate 20
Valve seat 30
Infusion port 1
Waste liquid port 2
Sequence testing chip 50
Second heat-conducting plate 60
The utility model embodiment that the following detailed description will be further explained with reference to the above drawings.
Specific embodiment
In order to be more clearly understood that the above objects, features, and advantages of the utility model embodiment, below with reference to attached
The utility model is described in detail in figure and specific embodiment.It should be noted that in the absence of conflict, the application
Embodiment in feature can be combined with each other.
Many details are explained in the following description in order to fully understand the utility model embodiment, it is described
Embodiment be only a part of embodiment of the utility model, rather than whole embodiment.Based in the utility model
Embodiment, every other embodiment party obtained by those of ordinary skill in the art without making creative efforts
Formula belongs to the range of the utility model embodiment protection.
Unless otherwise defined, all technical and scientific terms used herein and belong to the utility model embodiment
The normally understood meaning of those skilled in the art is identical.Terminology used in the description of the utility model herein
It is only for the purpose of describing specific embodiments, it is not intended that in limitation the utility model embodiment.
Gene sequencing system, for detecting the sequence rule of the base-pair on gene molecule or nucleic acid molecules, analysis prediction disease
Disease and risk.The system will be by that will be sequenced core dissolved with the different liquids reactant load of gene molecule or nucleic acid molecules
On piece is sufficiently reacted, so that sequence testing chip surface carries several gene molecules or nucleic acid molecules main chain according to certain rules, then
Group on each main chain is subjected to base pairing one by one, fluorescent marker, is taken pictures, to obtain complete sequence.It wherein will be molten
It is primary committed step that solution, which has the different liquids reactant of gene molecule or nucleic acid molecules to be loaded on sequence testing chip, after determining
The sequencing throughput and sequencing quality of phase.
Because sequence testing chip 50 needs and a variety of different reagents carry out biological respinse, existing pre-packaged chip loading method, envelope
It is high and cumbersome to fill processing cost, scrappage is high, the utilization rate of sequence testing chip 50 is low, and can not prevent the entrance of bubble, will affect
The progress (sequence testing chip 50 is unable to ingress of air in reaction) of biochemical reaction is unfavorable for quick, big throughput automated biological respinse,
So the utility model devises the loading device 100 of c-type flow passage cover plate formula accordingly.
The overall structure of the loading device 100 to the utility model is made into detailed elaboration below with reference to Fig. 1 to Fig. 5.
The device 100 carries out biochemical reaction for loading on liquid reactant on sequence testing chip, application is so that sequencing
Chip 50 does not need expensive encapsulation process, and reactant can be loaded directly on naked sequence testing chip 50, and at low cost, it is convenient to operate.
Fig. 1 shows the main structure of the device 100, specifically includes that
Cover board 10 is covered on sequence testing chip 50, forms sealing structure with sequence testing chip 50；
Sequence testing chip 50 is placed between cover board 10 and pedestal, and external equipped with clamping piece (not shown), convenient for taking
It takes；
Pedestal supports sequence testing chip 50 from bottom, and provides heat source and installation solenoid valve (not shown).
Wherein, pedestal further includes the first heat-conducting plate 20 and valve seat 30, and the left side of valve seat 30, right edge are equipped with electromagnetism outside
Valve (not shown), front side edge are equipped with infusion port 1 and waste liquid port 2.
For sealing structure is described in detail, integrally-built internal component shown in FIG. 1 is decomposed along longitudinal direction, such as Fig. 2 and Fig. 3
It is shown.
In present embodiment, it is close which from top to bottom is successively arranged cover board 10, sealing element 11, second
Sealing 12, sequence testing chip 50, the first heat-conducting plate 20, valve seat 30, the second heat-conducting plate 60.
Please refer to fig. 5, offering seal groove 15 on 10 bottom surface of cover board.In present embodiment, seal groove 15 is
The groove of square on 10 bottom surface of cover board, the groove is in convex shape (as shown in Figure 4), and the sealing element 11 is also accordingly
Convex shape can be completely embedded into seal groove 15 at the top of sealing element 11, and its bottom contacts on the sequence testing chip 50, from
And rectangular cavity is formed between the cover board 10, the sequence testing chip 50, the sealing element 11, the cover board 10 and the survey
Gap between sequence chip 50 is controlled 100 microns or less (for the cavity of conventional vessel, the gap is minimum).
It is appreciated that the shape of the seal groove 15, sealing element 11 is not limited to present embodiment；In other embodiments
In, the shape of the cavity be not limited to it is rectangular, can be it is round or other.
As shown in figure 5, being additionally provided with flow path groove 16 on 10 bottom surface of cover board, the flow path groove 16 is set to the seal groove
15 inside.In present embodiment, the flow path groove 16 is equipped with 2, the straight stream of a side for being parallel to seal groove 15
Road slot 16, another is c-type flow path groove 16 corresponding thereto, is c-type structure, and end is opened up to the midpoint of vertical edges, 2 streams
The inner side edge of road slot 16 and neighbouring seal groove 15 has certain distance, and both seal groove 15 and flow path groove 16 are not turned on.Flow path groove
16 effect is effectively to exclude gas, and sequence testing chip 50 is contacted with air when avoiding changing liquid, and liquid is guided to flow to.
It is appreciated that the end position of c-type flow path groove 16 is not limited to present embodiment；In other embodiments, institute
It states flow path groove 16 and not necessarily corresponds to liquid approach axis and discharge direction.
From Fig. 5 it is further seen that, the flow path groove 16 be equipped with an inlet 161 and several liquid outlets (162,163,
164), as shown in figure 4, the inlet 161 or the liquid outlet (162,163,164) pass through the corresponding stream inside the cover board
The seal groove 15 is crossed in road, and is connected external.The inlet 161 or the liquid outlet (162,163,164) and outside
Interface be equipped with second seal 12, the second seal 12 is embedded in inside the cover board 10.It is described in present embodiment
The end of flow path groove 16 is equipped with 1 inlet 161 and 3 liquid outlet (162,163,164) altogether, is located at the 4 of seal groove 15
A inner corner region.Correspondingly second seal 12 is equipped with 4, also to fall convex shape.In conjunction with Fig. 4 as it can be seen that 4 second seals 12
End contacts on valve seat 30, in this way, the solenoid valve (not shown) loaded on inlet 161 or liquid outlet and valve seat 30
Conducting.
It is appreciated that the quantity of liquid outlet (162,163,164) is not limited to present embodiment；In other embodiments
In, the position of the inlet 161, quantity are not limited to present embodiment.
10 top edge of cover board shown in Fig. 2 is equipped with perforative tapped through hole 14, opposite with the hole on the first heat-conducting plate 20
It answers, for the screw-threaded coupling of the two, realizes the regulation of the depth of cavity.
It is appreciated that cover board 10 and the first heat-conducting plate 20 not necessarily screw-threaded coupling, is also possible to top clamping up and down, or be
Other modes, it is only necessary to meet fastening function and adjusting function.
The valve seat 30 is hollow structure.In present embodiment, 30 lateral wall of valve seat is equipped with 5 solenoid valves
(not shown), including 1 liquid feed valve, 1 exhaust valve and 3 tapping valves, wherein the liquid feed valve and infusion port 1, described
Inlet 161 is connected conducting into liquid feeding flow path with pipeline, each tapping valve and a corresponding liquid outlet and waste liquid
Mouth 2 is connected with pipeline is connected liquid stream road in a row, the exhaust valve and the infusion port 1, the liquid feed valve, at least one drain
Valve and the waste liquid port 2 are connected with pipeline to be connected into exhaust flow path.In specific embodiment, liquid feed valve and infusion port 1 are apart from most
Close and conducting, third tapping valve are nearest apart from the waste liquid port 2.Wherein, the inlet 161 on straight flow path groove 16, liquid feed valve
And infusion port 1 is connected, the first liquid outlet 162, the first tapping valve, waste liquid port 2 on straight flow path groove 16 are connected, c-type flow path groove
The second liquid outlet 163, the second tapping valve, waste liquid port 2 on 16 are connected, third liquid outlet 164, third on c-type flow path groove 16
Tapping valve, waste liquid port 2 are connected, and the Single port of exhaust valve is connected with liquid feed valve, and the another port of exhaust valve and 3 drain flow paths
It simultaneously turns on, so exhaust valve can remove the gas and liquid to be changed (various reactants) in pipeline.Due to c-type flow path groove
It is connected between second liquid outlet 163 (corner) and neighbouring end, correspondingly, the third liquid outlet 164 of c-type flow path groove (turns
At angle) and neighbouring end between be also switched on, on the one hand this runner section extended can be played in fluid injection or while changing liquid draws
The effect of stream solves the problems, such as that border district changes that liquid is insufficient, on the other hand provides the channel that one is bubble water conservancy diversion, can be auxiliary
Help the bubble for being discharged into liquid (reaction reagent) loading zone.
It is appreciated that the quantity of inlet 161, liquid outlet and position are not limited, and correspondingly, the quantity of the solenoid valve
It is not limited to above-mentioned configuration with function, can satisfy exhaust, liquid feeding, drain operation.
The sequence testing chip 50 is placed on first heat-conducting plate 20, and top surface is surrounded to be formed with sealing element 11 by cover board 10
Cavity.In present embodiment, the sequence testing chip 50 of cavity sealing area be it is rectangular, 50 edge card of sequence testing chip is set to described
On first heat-conducting plate 20,50 top surface of sequence testing chip is concordant with the side upper surface of first heat-conducting plate 20.The sequencing
Chip 50 stretches out the region of first heat-conducting plate 20 for sector, and the sector portion is equipped with clamping piece, and specifically, clamping piece is set
It above sequence testing chip 50, is fixed in a manner of adhesion, convenient for taking, does not pollute the survey for having carried gene molecule or nucleic acid molecules
50 surface of sequence chip.
It is appreciated that the shape of the sequence testing chip 50 is not limited to present embodiment, meet utilization rate as high as possible
With ensure to clamp.
The bottom of first heat-conducting plate 20 is contacted with second heat-conducting plate 60, and from the bottom of the second heat-conducting plate 60 to
Screwing is fixed, and the side upper surface of first heat-conducting plate 20 is contacted with the cover board 10 and screw is fixed, and side following table
Face contacts and engages with the valve seat 30.The cover board 10 is equipped with positioning through hole 13, is positioned with the first heat-conducting plate 20 with bolt.
First heat-conducting plate 20, the second heat-conducting plate 60 shape set according to actual needs, therefore not to repeat here.
It is further specifically illustrated below with reference to liquid feeding process work of the Fig. 5 to the loading device 100 of the utility model.
Step 1: for the first time when liquid feeding, opening the exhaust valve, suck liquid, remove pipeline air；
Step 2: opening the liquid feed valve, the first tapping valve, and close exhaust valve, liquid flows to the along straight flow path groove 16
One liquid outlet 162；
Step 3: when liquid reaches first liquid outlet 162, the first tapping valve of closing, and the second tapping valve of unlatching, liquid
Body flows to the second liquid outlet 163 from the tiling of straight flow path groove 16, and is sufficient filling with the second liquid outlet 163 to neighbouring c-type flow path groove
Runner between 16 end；
Step 4: when liquid the second liquid outlet 163 of arrival, closing the second tapping valve, and open third tapping valve, liquid is filled out
Full c-type flow path groove 16 closes the liquid feed valve and the third tapping valve waits for quietly instead to third liquid outlet 164 and the other end
It should be complete.
It is further specifically illustrated below with reference to change liquid process work of the Fig. 5 to the loading device 100 of the utility model.
Step 1: opening the exhaust valve, suck new liquid, replace the liquid (waste liquid) reacted in pipeline；
Step 2: opening the liquid feed valve, the first tapping valve, and close exhaust valve, liquid flows to the along straight flow path groove 16
One liquid outlet 162, while drain flow path is extracted or be expressed into waste liquid out from the first liquid outlet 162, until being discharged from waste liquid port 2；
Step 3: when liquid reaches first liquid outlet 162, the first tapping valve of closing, and the second tapping valve of unlatching, liquid
Body tiles along straight flow path groove 16 and flows to the end of the second liquid outlet 163 and its neighbouring c-type flow path groove 16, at the same waste liquid from
Drain flow path is extracted or is expressed into out in second liquid outlet 163, until being discharged from waste liquid port 2；The unlatching of this second tapping valve of stage
The opening time is longer when time need to be than liquid feeding for the first time, mainly since the viscosity of liquid itself causes the emptying speed of velocity ratio gas
Degree wants low.
Step 4: when liquid the second liquid outlet 163 of arrival, closing the second tapping valve, and open third tapping valve, liquid is filled out
Full c-type flow path groove 16 is to third liquid outlet 164 and its other end, while waste liquid is extracted out or squeezed out from third liquid outlet 164
To drain flow path, until being discharged from waste liquid port 2, closes the liquid feed valve and the third tapping valve waits for fully reacting quietly.
To sum up, using the loading method of the device, simply, efficiently, loading procedure bubble-free, it is steady to change liquid, fringe region
It changes that liquid is more abundant, is conducive to the automatic biological reaction of quick, big flux.
The gene sequencing system of the application provided by the utility model loading device 100 further includes fluid system, heating system
System.
Wherein:
The fluid system is connected to the infusion port 1, and the opening and closing of solenoid valve described in automatic program control；
The heating system contacts to provide and control the required temperature of reaction with the bottom of second heat-conducting plate 60.
Various liquid reactants can be loaded on naked sequence testing chip 50 by the system automatically, change liquid processing efficient, stabilization, nothing
Bubble, not only increases the utilization rate of sequence testing chip 50, and reaction flux is big；And the need of repeated authentication test can be realized in single
It wants, testing efficiency is high, and test result is more acurrate；Loading procedure control is automated simultaneously, is designed in conjunction with seal cavity, so that repeatedly
The systematic error of load is small, and test result is more reliable.
Embodiment of above is only to illustrate the technical solution of the utility model embodiment rather than limits, although referring to more than
The utility model embodiment is described in detail in better embodiment, those skilled in the art should understand that, it can
It is modified with the technical solution to the utility model embodiment or equivalent replacement should not all be detached from the utility model embodiment
The spirit and scope of technical solution.
Claims (10)
1. a kind of loading device carries out biochemical reaction for loading on liquid reactant on sequence testing chip, it is characterised in that: packet
Cover board and pedestal are included, seal groove and flow path groove are offered on the cover board bottom surface, the flow path groove is set to the interior of the seal groove
Side, and an at least c-type structure, the cover board are contacted on the sequence testing chip simultaneously by being embedded in the sealing element of the seal groove
Cavity is formed, the flow path groove is equipped with inlet and liquid outlet, and the inlet or the liquid outlet pass through inside the cover board
Correspondence runner cross over the seal groove, and be connected to the solenoid valve on the pedestal, the solenoid valve for control liquid feeding with
Drain.
2. loading device according to claim 1, it is characterised in that: the cavity be it is rectangular, the flow path groove be equipped with one
Straight trough and C slot with its face, the flow path groove are equipped with several inlets and liquid outlet, the inlet or the liquid outlet
Second seal is equipped with the interface of the pedestal.
3. loading device according to claim 2, it is characterised in that: the end of the straight trough be respectively equipped with an inlet and
One liquid outlet.
4. loading device according to claim 2, it is characterised in that: the corner of the C slot is respectively equipped with a liquid outlet.
5. loading device according to claim 2, it is characterised in that: the solenoid valve include corresponding number liquid feed valve,
Tapping valve and an exhaust valve, wherein the liquid feed valve is connected conducting at liquid feeding flow path, often with pipeline with infusion port, the inlet
One tapping valve, which is connected with a corresponding liquid outlet and waste liquid port with pipeline, is connected liquid stream road in a row, the exhaust
Valve is connected conducting into exhaust stream with pipeline with the infusion port, the liquid feed valve, at least one tapping valve and the waste liquid port
Road.
6. loading device according to claim 1, it is characterised in that: it is thermally conductive that the pedestal is successively arranged first from top to bottom
Plate, valve seat and the second heat-conducting plate, the valve seat are hollow structure, and the bottom of first heat-conducting plate connects with second heat-conducting plate
It touches and fixed, the side upper surface of first heat-conducting plate is contacted with the cover board and screw is fixed, and lower surface and the valve
Seated connection touching.
7. loading device according to claim 6, it is characterised in that: the top surface of first heat-conducting plate is connected equipped with side
External groove sets the sequence testing chip for card, and after installation the sequence testing chip top surface and first heat-conducting plate side
Side upper surface is concordant.
8. loading device according to claim 7, it is characterised in that: the recess outlet of first heat-conducting plate is for described
The stretching of sequence testing chip.
9. loading device according to claim 1, it is characterised in that: solid by screw between the cover board and the pedestal
The fixed depth adjustment to realize cavity, the cover board are equipped with positioning through hole.
10. a kind of gene sequencing system, it is characterised in that: including fluid system, heating system and as in claim 1 to 9
Described in any item loading devices, the fluid system are connected to the infusion port of the loading device, and automatic program control institute
The opening and closing of solenoid valve are stated, the heating system contacts to provide simultaneously with the bottom of the second heat-conducting plate of the loading device
Temperature needed for control reaction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821327073.1U CN208883882U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821327073.1U CN208883882U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
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| Publication Number | Publication Date |
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| CN208883882U true CN208883882U (en) | 2019-05-21 |
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ID=66509121
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| CN201821327073.1U Active CN208883882U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020103126A1 (en) * | 2018-11-23 | 2020-05-28 | 深圳华大智造科技有限公司 | Gene sequencing reaction platform, sequencing chip and related method and system |
| WO2024021464A1 (en) * | 2022-07-26 | 2024-02-01 | 深圳太古语科技有限公司 | Method for surface chemical treatment and packaging bonding of sequencing chip |
-
2018
- 2018-08-16 CN CN201821327073.1U patent/CN208883882U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020103126A1 (en) * | 2018-11-23 | 2020-05-28 | 深圳华大智造科技有限公司 | Gene sequencing reaction platform, sequencing chip and related method and system |
| CN113242904A (en) * | 2018-11-23 | 2021-08-10 | 深圳华大智造科技股份有限公司 | Gene sequencing reaction platform, sequencing chip, and related method and system |
| CN113242904B (en) * | 2018-11-23 | 2024-04-30 | 深圳华大智造科技股份有限公司 | Gene sequencing reaction platform, sequencing chip, and related methods and systems |
| WO2024021464A1 (en) * | 2022-07-26 | 2024-02-01 | 深圳太古语科技有限公司 | Method for surface chemical treatment and packaging bonding of sequencing chip |
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