CN208857264U - Loading device and gene sequencing system - Google Patents
Loading device and gene sequencing system Download PDFInfo
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- CN208857264U CN208857264U CN201821327060.4U CN201821327060U CN208857264U CN 208857264 U CN208857264 U CN 208857264U CN 201821327060 U CN201821327060 U CN 201821327060U CN 208857264 U CN208857264 U CN 208857264U
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Abstract
The utility model provides a kind of loading device, for several liquid reactants to be loaded on naked sequence testing chip, including rotating disk, the waste liquid disk being fixedly installed in below the rotating disk, locating slot is opened up in the middle part of the rotating disk, the locating slot is for installing the sequence testing chip, the surrounding of the rotating disk opens up the first groove, and first groove extends downwardly out drain runner from bottom, the waste liquid disk opens up the second groove, and the drainage hole that drain runner port opens up on the waste liquid disk in correspondence first recess region.The application of the device is not so that sequence testing chip needs expensive encapsulation process, reactant can be loaded directly into changing on naked sequence testing chip that liquid is steady, make full use of the effective area of sequence testing chip, utilization rate is conducive to the automatic biological reaction of quick, big flux close to 100%.The utility model also provides a kind of gene sequencing system.
Description
Technical field
The utility model relates to gene technology fields, particularly relate to a kind of for several liquid reactants to be loaded on naked survey
The loading device of biochemical reaction, and the gene sequencing system using the device are carried out on sequence chip.
Background technique
This part intends to provides background or context for the embodiments of the present invention stated in claims.This
The description at place recognizes it is the prior art not because not being included in this section.
Gene sequencing chip (such as silicon wafer) needs and a variety of different reagents carry out biological respinse and can fix on its surface
Or gene molecule or nucleic acid molecules are carried, liquid speed degree is changed, liquid measure is changed, changes the liquid time, change liquid temperature, change liquid during load reagent
Stationarity requires strict control, and chip is unable to ingress of air during replacing reagent, so chip loads reaction process
It needs to carry out inside the locking device being especially tailored.By taking the load of gene nano sphere molecule (DNB) as an example, prior art
It is a kind of closed DNB loading method of silicon wafer, each chip is carried out special processing in advance and encapsulates (chip front side first by this method
Fixed position lid sets sheet glass, and edge glue dispensing and packaging makes internal formation runner, and wherein runner both ends, which correspond to, opens up feed liquor on chip
Mouth and liquid outlet, and combined chip-glass piece is fixed using frame), DNB or reagent are connected to feed liquor by the chip after encapsulation
Mouthful, the syringe drawing liquid through liquid outlet generates negative pressure, and reactant carries out biological respinse from inlet suction passage and after being full of, after
Reactant is until the reaction is complete in continuous drawing liquid replacement runner.
The encapsulation process of above-mentioned silicon wafer DNB loading method, each chip is complicated, and the precision of runner, sealing requirements
High, the scrappage of chip is high when encapsulation；Auxiliary material (sheet glass, frame) demand is big simultaneously, at high cost；Moreover, each
Chip is both needed to encapsulation process, is unfavorable for flexibly using, and especially chip is difficult to exclude after air inlet bubble, and after packaged glass piece not
Throughput automated biological respinse quick conducive to progress, big.It needs in the existing scheme for the load of naked sequence testing chip in sequence testing chip
Upper capping cover board, and in edge, dispensing is sealed, and opens up the bubble that flow path groove drains emptying into cavity, needs to match in this scheme
Standby complicated peripheral auxiliary equipment increases cost, and cover board and glue can contact reagent, still remain the risk of pollution chip.
Utility model content
In view of the foregoing, it is necessary to a kind of reagents loaded device for the naked sequence testing chip of large scale be provided and gene is surveyed
Sequence system, can carry out quick, big throughput automated biological respinse, and sequence testing chip utilization rate is close to 100%.
Technical solution provided by the utility model are as follows: a kind of loading device, for liquid reactant to be loaded on sequencing core
On piece carries out biochemical reaction, including rotating disk, the waste liquid disk being fixedly installed in below the rotating disk, the middle part of the rotating disk
Locating slot is opened up, for the locating slot for installing the sequence testing chip, the surrounding of the rotating disk opens up the first groove, and described
First groove extends downwardly out drain runner from bottom, and it is recessed that the waste liquid disk in correspondence first recess region opens up second
Slot, and the drainage hole that drain runner port opens up on the waste liquid disk.
Further, the waste liquid disk be hollow structure disk, the cover board, the rotating disk, the waste liquid disk with
The sequence testing chip coaxial arrangement.
Further, the inside of the waste liquid disk is equipped with mounting groove, is engaged with driver, and by the driver drives
It rotates about the axis.
Further, circumferential direction is uniformly distributed 4 mounting grooves in the inside circumference of the waste liquid disk.
Further, the drain runner is equipped with 2, is distributed in the both ends of one diameter of the first groove circumference.
Further, the drainage hole is equipped with 2, respectively corresponds a drain runner.
Further, several blind holes are opened up on the rotating disk top surface of first groove inside edge, for installing
Spring, so that the cover board with top retains the gap between the cover board and the sequence testing chip while fixation with screw.
Further, several through-holes are opened up on the rotating disk top surface of first groove inside edge, with it is described
Driver is fixed at the mounting groove with screw.
Further, the cover board top surface is circle, and the cover plate central opens up inlet opening.
The utility model also provides a kind of gene sequencing system, impregnates reaction test system, including stream for naked sequence testing chip
System system and the loading device, automatic program control fluid injection, drawing liquid and the load of the fluid system to set
The rotation of device.
Compared with prior art, provided by the utility model for several liquid reactants to be loaded on naked sequence testing chip
Loading device, including rotating disk, the waste liquid disk being fixedly installed in below the rotating disk, it is fixed to open up in the middle part of the rotating disk
Position slot, for the locating slot for installing the sequence testing chip, the surrounding of the rotating disk opens up the first groove, and described first recessed
Slot extends downwardly out drain runner from bottom, and the waste liquid disk opens up the second groove, and institute in correspondence first recess region
State the drainage hole that drain runner port opens up on the waste liquid disk.The application of the device is not so that sequence testing chip needs valuableness
Encapsulation process, reactant can be loaded directly on naked sequence testing chip, and at low cost, it is convenient to operate, can be complete using centrifugal action
At load, simply, efficiently, bubble-free changes that liquid is steady, takes full advantage of the response area of large scale circle sequence testing chip, favorably
It is reacted in the automatic biological of quick, big flux；Differential responses agent can be loaded onto automatically using its gene sequencing system naked
On sequence testing chip, it being made to carry gene molecule or nucleic acid molecules, improves the utilization rate of round sequence testing chip, reaction flux is big,
Expanded application.
Detailed description of the invention
Utility model will be further described in detail below with reference to the attached drawings and specific embodiments.
Fig. 1 is the perspective view of the loading device of one embodiment of the utility model.
Fig. 2 is the exploded view that loading device shown in FIG. 1 is presented from a visual angle.
Fig. 3 is the exploded view that loading device shown in FIG. 1 is presented from another visual angle.
Description of symbols:
Loading device 100
Cover board 10
Rotating disk 11
Locating slot 12
First groove 21
Inlet opening 161
Drainage hole 162
Drain runner 163
Waste liquid disk 30
Mounting groove 31
Second groove 32
Sequence testing chip 50
The utility model embodiment that the following detailed description will be further explained with reference to the above drawings.
Specific embodiment
In order to be more clearly understood that the above objects, features, and advantages of the utility model embodiment, below with reference to attached
The utility model is described in detail in figure and specific embodiment.It should be noted that in the absence of conflict, the application
Embodiment in feature can be combined with each other.
Many details are explained in the following description in order to fully understand the utility model embodiment, it is described
Embodiment be only a part of embodiment of the utility model, rather than whole embodiment.Based in the utility model
Embodiment, every other embodiment party obtained by those of ordinary skill in the art without making creative efforts
Formula belongs to the range of the utility model embodiment protection.
Unless otherwise defined, all technical and scientific terms used herein and belong to the utility model embodiment
The normally understood meaning of those skilled in the art is identical.Terminology used in the description of the utility model herein
It is only for the purpose of describing specific embodiments, it is not intended that in limitation the utility model embodiment.
Gene sequencing system, for detecting the sequence rule of the base-pair on gene molecule or nucleic acid molecules, analysis prediction disease
Disease and risk.The system will be by that will be sequenced core dissolved with the different liquids reactant load of gene molecule or nucleic acid molecules
On piece is sufficiently reacted, so that sequence testing chip surface carries several gene molecules or nucleic acid molecules main chain according to certain rules, then
Group on each main chain is subjected to base pairing one by one, fluorescent marker, is taken pictures, to obtain complete sequence.It wherein will be molten
It is primary committed step that solution, which has the different liquids reactant of gene molecule or nucleic acid molecules to be loaded on sequence testing chip, after determining
The sequencing throughput and sequencing quality of phase.
Because sequence testing chip 50 needs and a variety of different reagents carry out biological respinse, existing pre-packaged chip loading method, envelope
It is high and cumbersome to fill processing cost, scrappage is high, the utilization rate of sequence testing chip 50 is low, and can not prevent the entrance of bubble, will affect
The progress (sequence testing chip 50 is unable to ingress of air in reaction) of biochemical reaction is unfavorable for quick, big throughput automated biological respinse.
So the utility model devises centrifugal loading device 100 accordingly.
The overall structure of the loading device 100 to the utility model is made into detailed elaboration below with reference to Fig. 1 to Fig. 3.
The device 100 carries out biochemical reaction for loading on liquid reactant on sequence testing chip, application is so that sequencing
Chip 50 does not need expensive encapsulation process, and reactant can be loaded directly on naked sequence testing chip 50, and at low cost, it is convenient to operate.
Fig. 1 shows the main structure of the device 100, specifically includes that
Cover board 10 is set to 50 top of sequence testing chip, positions for top fluid injection；
Rotating disk 11 for supporting sequence testing chip 50 from bottom, and drives sequence testing chip 50 to rotate together, makees by centrifugation
With paving liquid；
Waste liquid disk 30, for collecting and waste liquid being discharged.
Sequence testing chip 50 is placed in rotating disk 11, for round (being not shown in the figure, in inside).
For the device 100 is described in detail, integrally-built internal component shown in FIG. 1 is decomposed along longitudinal direction, such as Fig. 2 and figure
Shown in 3.
In present embodiment, which is successively arranged cover board 10, rotating disk 11, sequencing core from top to bottom
Piece 50, waste liquid disk 30, are circular configuration, coaxial arrangement.
The cover board 10 is circle, and center offers inlet opening 161.In present embodiment, inlet opening 161 is equipped with 1,
Section is circle.It is appreciated that the cross sectional shape of the cover board 10 is not limited to circle, it is only necessary to realize from center injection liquid examination
Agent such as need to be sized at the parameters depending on actual conditions.
The rotating disk 11 is circle, and middle part opens up locating slot 12, and the locating slot 12 is for installing the sequencing core
Piece 50.In present embodiment, the locating slot 12 is strip.The surrounding of the rotating disk 11 opens up the first groove 21.This reality
It applies in mode, first groove 21 is connected to the locating slot 12, and bottom surface is concordant, and first groove 21 is two stage steps
Shape, outside bottom surface are lower than inside bottom surface；And it is opened up on the rotating disk top surface of 21 inside edge of the first groove several blind
Hole while so that the cover board 10 with top is fixed with screw, retains the cover board 10 and the sequencing for installing spring
Gap between chip 50, in other words, the cover board 10 are not in contact with each other with the sequence testing chip 50, the diameter of the cover board 10
Greater than the diameter of the sequence testing chip 50.In present embodiment, 4 circumferential directions are opened up on the circumference of the cover board 10 and are uniformly distributed
Hole, it is corresponding with blind hole.The effect of the cover board 10 is to prevent liquid splash.
It is appreciated that the connection type of the cover board 10 and the sequence testing chip 50 is not limited to present embodiment, it is described
Hole on cover board 10, the blind hole in the rotating disk 11 quantity be also possible to 3,5 or more；Form bullet used in gap
Spring is also possible to pin etc.；When being furnished with perpendicular positioning liquid injection pipe, in the case where not considering liquid splash, the cover board 10 can also
Not use.
First groove 21 extends downwardly out drain runner 163 from bottom.Drain runner described in present embodiment 163
Equipped with 2, hollow circular is distributed in the both ends of 21 circumference of the first groove, one diameter.It is appreciated that the drain runner
163 shape, quantity, position are not limited to present embodiment, it is only necessary to meet the waste liquid for converging the first groove 21 and be discharged.
The waste liquid disk 30 is fixedly installed in 11 lower section of rotating disk.The waste liquid disk 30 is recessed in correspondence described first
21 region of slot opens up the second groove 32, and the drainage hole that 163 port of drain runner opens up on the waste liquid disk 30
162.In present embodiment, the drain runner 163 is equipped with 2, and accordingly drainage hole 162 also is provided with 2.As shown in figure 3, institute
The disk that waste liquid disk 30 is hollow structure is stated, the inside of the waste liquid disk 30 is equipped with mounting groove 31, is engaged with driver, and
It is rotated about the axis by the driver drives.It can also be seen that the institute of 21 inside edge of the first groove in conjunction with Fig. 2 and Fig. 3
It states and opens up several through-holes on 11 top surface of rotating disk, be fixed with the driver at the mounting groove 31 with screw.
The liquid feeding process work of the loading device of the utility model 100 is further specifically illustrated below.
For the first time when liquid feeding (paving liquid), it is passed through liquid in inlet opening 161, opening driver driving device 100 rotates, and liquid arrives
It up to 50 surface of sequence testing chip, sprawls under the action of the centrifugal force, until the surface for being paved with entire sequence testing chip 50 is reacted.
The liquid process work that changes of the loading device of the utility model 100 is further specifically illustrated below.
Fluid system replaces liquid reactant, continues to inject from inlet opening 161, synchronous to open drainage hole 162, new liquid
Liquid is gradually replaced at 50 center of circle of sequence testing chip to edge under the action of the centrifugal force, while waste liquid is discharged；
To sum up, using the loading procedure of the device, simply, efficiently, change that liquid is steady, and 50 surface of sequence testing chip is without partition, instead
Answer agent that can sufficiently react with sequence testing chip 50, utilization rate is up to 100%, suitable for the reagents loaded of large scale circle sequence testing chip,
Be conducive to the automatic biological reaction of quick, big flux.
The gene sequencing system of the application provided by the utility model loading device 100, further includes fluid system, the stream
System system includes driving mechanism, the rotation for loading device described in the automatic program control with setting；It can also further set
There are solenoid valve, solenoid valve connection inlet opening 161 (or drainage hole 162) and liquid pump, the open and close of the solenoid valve are used
In the liquid feeding (or liquid out) for controlling the loading device, the solenoid valve, the driving mechanism by the fluid system control
Molding block regulates and controls state.
Various liquid reactants can be loaded on naked sequence testing chip 50 by the system automatically, improve the benefit of sequence testing chip 50
With rate, it is big to react flux, and round sequence testing chip 50 can sufficiently change liquid, extend the application of round sequence testing chip 50；Simultaneously
Loading procedure control is automated, so that the systematic error of repeated loading is small, test result is more reliable.
Embodiment of above is only to illustrate the technical solution of the utility model embodiment rather than limits, although referring to more than
The utility model embodiment is described in detail in better embodiment, those skilled in the art should understand that, it can
It is modified with the technical solution to the utility model embodiment or equivalent replacement should not all be detached from the utility model embodiment
The spirit and scope of technical solution.
Claims (10)
1. a kind of loading device carries out biochemical reaction for loading on liquid reactant on sequence testing chip, it is characterised in that: packet
The waste liquid disk for including rotating disk, being fixedly installed in below the rotating disk opens up locating slot, the positioning in the middle part of the rotating disk
Slot is for installing the sequence testing chip, and the surrounding of the rotating disk opens up the first groove, and first groove is downward from bottom
Extend drain runner, the waste liquid disk opens up the second groove, and drain runner end in correspondence first recess region
The drainage hole that mouth opens up on the waste liquid disk.
2. loading device according to claim 1, it is characterised in that: the waste liquid disk is the disk of hollow structure, described
Rotating disk, the waste liquid disk and the sequence testing chip are coaxially disposed.
3. loading device according to claim 1, it is characterised in that: the inside of the waste liquid disk be equipped with mounting groove, with
Driver engaging, and rotated about the axis by the driver drives.
4. loading device according to claim 3, it is characterised in that: circumferential in the inside circumference of the waste liquid disk uniformly to divide
4 mounting grooves of cloth.
5. loading device according to claim 1, it is characterised in that: the drain runner is equipped with 2, is distributed in described the
The both ends of one groove circumference, one diameter.
6. loading device according to claim 1, it is characterised in that: the drainage hole is equipped with 2, respectively corresponds described in one
Drain runner.
7. loading device according to claim 1, it is characterised in that: the rotating disk of first groove inside edge
Several blind holes are opened up on top surface, for installing spring, so that the cover board with top retains the cover board while fixed with screw
With the gap between the sequence testing chip.
8. loading device according to claim 3, it is characterised in that: the rotating disk of first groove inside edge
Several through-holes are opened up on top surface, are fixed with the driver at the mounting groove with screw.
9. loading device according to claim 7, it is characterised in that: the cover board top surface is circle, the cover plate central
Open up inlet opening.
10. a kind of gene sequencing system, it is characterised in that: including fluid system and such as any one of claims 1 to 9 institute
The loading device stated, the fluid system is with the automatic program control fluid injection set, the rotation of drawing liquid and the loading device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821327060.4U CN208857264U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821327060.4U CN208857264U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
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| Publication Number | Publication Date |
|---|---|
| CN208857264U true CN208857264U (en) | 2019-05-14 |
Family
ID=66414844
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| Application Number | Title | Priority Date | Filing Date |
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| CN201821327060.4U Active CN208857264U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021056208A1 (en) * | 2019-09-24 | 2021-04-01 | 深圳华大智造科技有限公司 | Biochemical substance analysis system, method, and device |
| WO2022262833A1 (en) * | 2021-06-17 | 2022-12-22 | Mgi Tech Co., Ltd. | Sequencing systems and methods utilizing curved imaging paths on rotating substrates |
| CN114341618B (en) * | 2019-09-24 | 2024-05-14 | 深圳华大智造科技股份有限公司 | Biochemical substance analysis system, method and device |
-
2018
- 2018-08-16 CN CN201821327060.4U patent/CN208857264U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021056208A1 (en) * | 2019-09-24 | 2021-04-01 | 深圳华大智造科技有限公司 | Biochemical substance analysis system, method, and device |
| CN114341618A (en) * | 2019-09-24 | 2022-04-12 | 深圳华大智造科技股份有限公司 | Biochemical substance analysis system, method and device |
| AU2019467369B2 (en) * | 2019-09-24 | 2023-10-19 | Mgi Tech Co., Ltd. | Biochemical substance analysis system, method, and device |
| CN114341618B (en) * | 2019-09-24 | 2024-05-14 | 深圳华大智造科技股份有限公司 | Biochemical substance analysis system, method and device |
| WO2022262833A1 (en) * | 2021-06-17 | 2022-12-22 | Mgi Tech Co., Ltd. | Sequencing systems and methods utilizing curved imaging paths on rotating substrates |
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Address after: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee after: Shenzhen Huada Zhizao Technology Co., Ltd Address before: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee before: Shenzhen Huada Zhizao Technology Co.,Ltd. |
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