CN208776710U - Loading device and gene sequencing system - Google Patents
Loading device and gene sequencing system Download PDFInfo
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- CN208776710U CN208776710U CN201821327067.6U CN201821327067U CN208776710U CN 208776710 U CN208776710 U CN 208776710U CN 201821327067 U CN201821327067 U CN 201821327067U CN 208776710 U CN208776710 U CN 208776710U
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- path groove
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Abstract
A kind of loading device and gene sequencing system provided by the utility model, pedestal including cover board and below the cover board, the arc side of the cover board bottom surface divides several segments, each section of arc side is along preset direction by the cover board subregion, the edge dispensing of each subregion to form independent flow path groove with being bonded with sequence testing chip, each flow path groove is equipped with inlet opening far from the end of arc side, the inlet opening extends air discharge duct in the flow path groove, gas vent is additionally provided on the air discharge duct, the flow path groove corresponds to arc side area to export or being equipped with fluid hole.The application of the device is not so that sequence testing chip needs expensive encapsulation process, reactant can be loaded directly on naked sequence testing chip, it is at low cost, it is convenient to operate, and it is steady to change liquid for loading procedure bubble-free, be conducive to the automatic biological reaction of quick, big flux, and open type leakage fluid dram can sufficiently exclude runner gas and waste liquid, guarantee paving liquid or to change liquid abundant, and it is practical.
Description
Technical field
The utility model relates to gene technology fields, particularly relate to a kind of for several liquid reactants to be loaded on naked survey
The loading device of biochemical reaction, and the gene sequencing system using the device are carried out on sequence chip.
Background technique
This part intends to provides background or context for the embodiments of the present invention stated in claims.This
The description at place recognizes it is the prior art not because not being included in this section.
Gene sequencing chip (such as silicon wafer) needs and a variety of different reagents carry out biological respinse and can fix on its surface
Or gene molecule or nucleic acid molecules are carried, liquid speed degree is changed, liquid measure is changed, changes the liquid time, change liquid temperature, change liquid during load reagent
Stationarity requires strict control, and chip is unable to ingress of air during replacing reagent, so chip loads reaction process
It needs to carry out inside the locking device being especially tailored.By taking the load of gene nano sphere molecule (DNB) as an example, prior art
It is a kind of closed DNB loading method of silicon wafer, each chip is carried out special processing in advance and encapsulates (chip front side first by this method
Fixed position lid sets sheet glass, and edge glue dispensing and packaging makes internal formation runner, and wherein runner both ends, which correspond to, opens up feed liquor on chip
Mouth and liquid outlet, and combined chip-glass piece is fixed using frame), DNB or reagent are connected to feed liquor by the chip after encapsulation
Mouthful, the syringe drawing liquid through liquid outlet generates negative pressure, and reactant carries out biological respinse from inlet suction passage and after being full of, after
Reactant is until the reaction is complete in continuous drawing liquid replacement runner.
The encapsulation process of above-mentioned silicon wafer DNB loading method, each chip is complicated, and the precision of runner, sealing requirements
High, the scrappage of chip is high when encapsulation；Auxiliary material (sheet glass, frame) demand is big simultaneously, at high cost；Moreover, each
Chip is both needed to encapsulation process, is unfavorable for flexibly using, and especially chip is difficult to exclude after air inlet bubble, and after packaged glass piece
It is unfavorable for carrying out quick, big throughput automated biological respinse.
Utility model content
In view of the foregoing, it is necessary to a kind of reagents loaded device for the naked sequence testing chip of large scale be provided and gene is surveyed
Sequence system can carry out quick, big throughput automated biological respinse.
Technical solution provided by the utility model are as follows: a kind of loading device, for liquid reactant to be loaded on sequencing core
On piece carries out biochemical reaction, the pedestal including cover board and below the cover board, and the arc side of the cover board bottom surface divides several
Section, along preset direction by the cover board subregion, the edge dispensing of each subregion to be formed each section of arc side with being bonded with sequence testing chip
Independent flow path groove, each flow path groove are equipped with inlet opening far from the end of arc side, and the inlet opening is in the flow path groove
Extend air discharge duct, gas vent is additionally provided on the air discharge duct, the flow path groove corresponds to arc side area to export or being equipped with fluid hole.
Further, the pedestal is used to support the sequence testing chip, and locks with the cover board to compress colloid realization
The isolation and sealing of each subregion.
Further, each flow path groove corresponds to arc side area as outlet.
Further, the cover board, which corresponds to, is divided on the bottom surface of the circular sequence testing chip by dispensing.
Further, the preset direction is the central axes direction of entire arc side, and the width of each flow path groove is solid
Definite value.
Further, each flow path groove is equipped with an inlet opening far from the end angular region of arc side.
Further, the air discharge duct is arranged along each flow path groove far from the tip inside of arc side.
Further, the end on the air discharge duct far from the inlet opening opens up gas vent.
Further, the edge of the sequence testing chip and the flow path groove seal, and the flow path groove corresponds to the end of arc side
Fluid hole is opened up, the discharge for waste liquid.
The utility model also provides a kind of gene sequencing system, described including fluid system and the loading device
Fluid system includes solenoid valve, and the open and close of the solenoid valve are for controlling the feed liquor and liquid out of the loading device.
Compared with prior art, provided by the utility model for several liquid reactants to be loaded on naked sequence testing chip
Loading device, the pedestal including cover board and below the cover board, the arc side of the cover board bottom surface divides several segments, each
For section arc side along preset direction by the cover board subregion, the edge dispensing of each subregion to form independent stream with being bonded with sequence testing chip
Road slot, each flow path groove are equipped with inlet opening far from the end of arc side, and the inlet opening extends row in the flow path groove
Air drain, gas vent is additionally provided on the air discharge duct, and the flow path groove corresponds to arc side area to export or being equipped with fluid hole.The device
Using so that sequence testing chip does not need expensive encapsulation process, reactant can be loaded directly on naked sequence testing chip, at low cost, behaviour
It is convenient to make, and loading procedure is simple, and efficiently, bubble-free changes that liquid is steady, suitable for the reagents loaded of large scale circle sequence testing chip, has
It is reacted conducive to the automatic biological of quick, big flux；Differential responses agent can be loaded onto automatically using its gene sequencing system
On naked sequence testing chip, it is made to carry gene molecule or nucleic acid molecules, improve the utilization rate of round sequence testing chip, reacts flux
Greatly, expanded application.
Detailed description of the invention
Utility model will be further described in detail below with reference to the attached drawings and specific embodiments.
Fig. 1 is the perspective view of the loading device of one embodiment of the utility model.
Fig. 2 is the exploded view that loading device shown in FIG. 1 is presented from a visual angle.
Fig. 3 is the exploded view that loading device shown in FIG. 1 is presented from another visual angle.
Fig. 4 is the bottom view of cover board shown in FIG. 1.
Description of symbols:
| Loading device | 100 |
| Cover board | 10 |
| Glue | 11 |
| Flow path groove | 16 |
| Inlet opening | 161 |
| Fluid hole | 162 |
| Air discharge duct | 15 |
| Gas vent | 1601 |
| Pedestal | 30 |
| Sequence testing chip | 50 |
The utility model embodiment that the following detailed description will be further explained with reference to the above drawings.
Specific embodiment
In order to be more clearly understood that the above objects, features, and advantages of the utility model embodiment, below with reference to attached
The utility model is described in detail in figure and specific embodiment.It should be noted that in the absence of conflict, the application
Embodiment in feature can be combined with each other.
Many details are explained in the following description in order to fully understand the utility model embodiment, it is described
Embodiment be only a part of embodiment of the utility model, rather than whole embodiment.Based in the utility model
Embodiment, every other embodiment party obtained by those of ordinary skill in the art without making creative efforts
Formula belongs to the range of the utility model embodiment protection.
Unless otherwise defined, all technical and scientific terms used herein and belong to the utility model embodiment
The normally understood meaning of those skilled in the art is identical.Terminology used in the description of the utility model herein
It is only for the purpose of describing specific embodiments, it is not intended that in limitation the utility model embodiment.
Gene sequencing system, for detecting the sequence rule of the base-pair on gene molecule or nucleic acid molecules, analysis prediction disease
Disease and risk.The system will be by that will be sequenced core dissolved with the different liquids reactant load of gene molecule or nucleic acid molecules
On piece is sufficiently reacted, so that sequence testing chip surface carries several gene molecules or nucleic acid molecules main chain according to certain rules, then
Group on each main chain is subjected to base pairing one by one, fluorescent marker, is taken pictures, to obtain complete sequence.It wherein will be molten
It is primary committed step that solution, which has the different liquids reactant of gene molecule or nucleic acid molecules to be loaded on sequence testing chip, after determining
The sequencing throughput and sequencing quality of phase.
Because sequence testing chip 50 needs and a variety of different reagents carry out biological respinse, existing pre-packaged chip loading method, envelope
It is high and cumbersome to fill processing cost, scrappage is high, the utilization rate of sequence testing chip 50 is low, and can not prevent the entrance of bubble, will affect
The progress (sequence testing chip 50 is unable to ingress of air in reaction) of biochemical reaction is unfavorable for quick, big throughput automated biological respinse.
So the utility model devises dispensing accordingly and divides flow channel type loading device 100.
The overall structure of the loading device 100 to the utility model is made into detailed elaboration below with reference to Fig. 1 to Fig. 4.
The device 100 carries out biochemical reaction for loading on liquid reactant on sequence testing chip, application is so that sequencing
Chip 50 does not need expensive encapsulation process, and reactant can be loaded directly on naked sequence testing chip 50, and at low cost, it is convenient to operate.
Fig. 1 shows the main structure of the device 100, specifically includes that
Cover board 10 is covered on sequence testing chip 50, forms flow passage structure with sequence testing chip 50；
Pedestal 30 supports sequence testing chip 50 from bottom；
Sequence testing chip 50 is placed between cover board 10 and pedestal 30, for round (being not shown in the figure, be clipped in inside).
For flow passage structure is described in detail, integrally-built internal component shown in FIG. 1 is decomposed along longitudinal direction, such as Fig. 2 and Fig. 3
It is shown.
In present embodiment, which is successively arranged cover board 10, sequence testing chip 50, pedestal from top to bottom
30。
Referring to Figure 4 together, the cover board 10 is the combination of square structure and arc, and arc side is one in 10 inner circle of cover board
Section, for arc angle less than 180 °, the arc side of 10 bottom surface of cover board divides several segments, and each section of arc side is along preset direction by the cover board
10 subregions.In present embodiment, arc side is divided into 12 sections, and each section of arc side is along the central axes direction of entire arc side by 10 bottom of cover board
Millet cake glue is divided into a subregion.More specifically, the width of 12 subregions is equal in present embodiment, both ends are mirror symmetry
Arc side.
It is appreciated that the width in partitioned mode (direction, path), each subregion is not limited to present embodiment, it is visual real
Border needs to adjust.
As shown in figure 4, the edge of each subregion carries out dispensing processing, one end is formed after being bonded with corresponding sequence testing chip
It opens wide, the independent flow path groove 16 of remaining sealing.In present embodiment, the flow path groove is equipped with 12, is strip, width one
It causes, independently of each other, does not interfere with each other.
Meanwhile each flow path groove 16 is equipped with inlet opening 161 far from the end of arc side, the inlet opening 161 is described
Extend air discharge duct 15 in flow path groove 16, is additionally provided with gas vent 1601 on the air discharge duct 15.It is each described in present embodiment
Flow path groove 16 is equipped with an inlet opening 161 far from the end angular region of arc side.The air discharge duct 15 is separate along each flow path groove 116
The tip inside of arc side is arranged.End on the air discharge duct 15 far from the inlet opening 161 opens up a gas vent 1601.It is described
Gas vent 1601, the inlet opening 161 are circle, and the former diameter is less than the latter.In corresponding present embodiment, inlet opening
161 have 12, and gas vent 1601 has 12, and air discharge duct 15 is also 12.The air discharge duct 15 differs in size, and shape is not
One.The effect of air discharge duct 15 is mainly from inevitably there is individual bubbles to enter when 161 fluid injection of inlet opening, since air discharge duct 15 is in cover board 10
Above, so the bubble entered can be flowed under buoyancy by air discharge duct 15, to be discharged from gas vent 1601.
It is appreciated that shape, the size of the air discharge duct 15 be not limited to present embodiment, slot length and width and depth according to
Actual needs can be changed；In other embodiments, quantity, position, the size of the inlet opening 161 and the gas vent 1601
It is not limited to present embodiment.
In the present embodiment, the corresponding arc side area of the flow path groove 16 is outlet, is fluid hole 162, open type drain
Speed is faster, more efficient；In another specific embodiment, it can be equipped between the sequence testing chip 50 and the cover board 10
Glue-line, the glue-line, at this time need to be in 16 arcs of flow path groove by the sequence testing chip 50, the flow path groove 16 and extraneous seal isolation
Fluid hole 162 is set at side so as to waste liquid discharge.
As shown in Figures 2 and 3, the pedestal 30 is rectangular, is matched with cover board 10.30 top surface of pedestal offers arc
Shape slot and strip groove, the strip groove are vertically intersected on the middle part of the arc groove, the end line of the arc groove with it is described
Strip groove intersection.
The sequence testing chip 50 is placed on the pedestal 30, and top surface is by 10 gluing of cover board and is divided into multizone.This implementation
In mode, sequence testing chip 50 is circle, is bonded with the cover board 10 and forms 12 subregion flow path grooves 16.Institute on pedestal 30 simultaneously
The clamping piece that strip groove is used to place sequence testing chip 50 is stated, the back side of sequence testing chip 50 is fixed in a manner of adhesion, convenient for taking,
50 surface of sequence testing chip for having loaded gene molecule or nucleic acid molecules is not polluted.
The liquid feeding process work of the loading device of the utility model 100 is further specifically illustrated below.
For the first time when liquid feeding (paving liquid), each subregion substep or simultaneous implantation liquid, liquid is first along corresponding flow path groove
16 tilings are sufficiently reacted until arrival fluid hole 162；The bubble for being mixed into flow path groove 16 simultaneously is moved along air discharge duct 15, until exhaust
Hole 1601 is discharged.It is open type leakage fluid dram formula in the utility model, leakage fluid dram effect is can to drain runner gas, guarantees chip
Runner spreads liquid effect.
The liquid process work that changes of the loading device of the utility model 100 is further specifically illustrated below.
Each subregion substep or the new liquid of simultaneous implantation, liquid tile along corresponding flow path groove 16 push waste liquid first
It to the movement of fluid hole 162 and is discharged, full of rear sufficiently reaction；The bubble for being mixed into flow path groove 16 simultaneously is moved along air discharge duct 15, until
Gas vent 1601 is discharged.It is open type leakage fluid dram formula in the utility model, effect can drain waste liquid when being change liquid, protect
A kind of liquid can a kind of liquid in full replacement in runner under card.
To sum up, using the loading procedure of the device, simply, efficiently, loading procedure eliminates the adverse effect of bubble, and it is flat to change liquid
Surely, the reagents loaded suitable for large scale circle sequence testing chip is conducive to the automatic biological reaction of quick, big flux.
The gene sequencing system of the application provided by the utility model loading device 100, further includes fluid system, the stream
System system is equipped with solenoid valve, and the solenoid valve is connect with the loading device；In present embodiment, the solenoid valve divides liquid feed valve
And exhaust valve, the liquid feed valve are connected into valve port with storage bottle, pump；The liquid feed valve goes out valve port and the inlet
161 with pipeline connection；Similarly, the gas vent 1601, the exhaust valve are conducted into valve port, air pump and outside.Work as presence
When fluid hole 162, tapping valve is also accordingly set, communicates therewith, waste liquid is discharged.
Various liquid reactants can be loaded on naked sequence testing chip 50 by the system automatically, not only increase sequence testing chip 50
Utilization rate, reaction flux it is big, round sequence testing chip 50 can sufficiently change liquid, extend the application of round sequence testing chip 50；And
And open type leakage fluid dram can sufficiently exclude runner gas and waste liquid, guarantee paving liquid or to change liquid abundant, it is practical；It automates simultaneously
Loading procedure control, so that the systematic error of repeated loading is small, test result is more reliable.
Embodiment of above is only to illustrate the technical solution of the utility model embodiment rather than limits, although referring to more than
The utility model embodiment is described in detail in better embodiment, those skilled in the art should understand that, it can
It is modified with the technical solution to the utility model embodiment or equivalent replacement should not all be detached from the utility model embodiment
The spirit and scope of technical solution.
Claims (10)
1. a kind of loading device carries out biochemical reaction for loading on liquid reactant on sequence testing chip, it is characterised in that: packet
Cover board and the pedestal below the cover board are included, the arc side of the cover board bottom surface divides several segments, and each section of arc side is along default
The cover board subregion, the edge dispensing of each subregion to be formed independent flow path groove, Mei Yisuo with being bonded with sequence testing chip by direction
It states flow path groove and is equipped with inlet opening far from the end of arc side, the inlet opening extends air discharge duct, the row in the flow path groove
Gas vent is additionally provided on air drain, the flow path groove corresponds to arc side area to export or being equipped with fluid hole.
2. loading device according to claim 1, it is characterised in that: the pedestal is used to support the sequence testing chip, and
With the cover board lock with compress colloid realize each subregion be isolated and sealing.
3. loading device according to claim 1, it is characterised in that: each flow path groove corresponds to arc side area as outlet.
4. loading device according to claim 1, it is characterised in that: the cover board corresponds to the circular sequence testing chip
It is divided on bottom surface by dispensing.
5. loading device according to claim 1, it is characterised in that: the preset direction is the central axes side of entire arc side
To the width of each flow path groove is fixed value.
6. loading device according to claim 1, it is characterised in that: end angular region of each flow path groove far from arc side
Equipped with an inlet opening.
7. loading device according to claim 6, it is characterised in that: the air discharge duct is along each flow path groove far from arc
The tip inside on side is arranged.
8. loading device according to claim 7, it is characterised in that: far from the end of the inlet opening on the air discharge duct
Open up gas vent.
9. loading device according to claim 8, it is characterised in that: the edge of the sequence testing chip and the flow path groove are close
Envelope, the end that the flow path groove corresponds to arc side open up fluid hole, the discharge for waste liquid.
10. a kind of gene sequencing system, it is characterised in that: including fluid system and such as any one of claims 1 to 9 institute
The loading device stated, the fluid system include solenoid valve, and the open and close of the solenoid valve are for controlling the load dress
The feed liquor set and out liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821327067.6U CN208776710U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821327067.6U CN208776710U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
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| Publication Number | Publication Date |
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| CN208776710U true CN208776710U (en) | 2019-04-23 |
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| CN201821327067.6U Active CN208776710U (en) | 2018-08-16 | 2018-08-16 | Loading device and gene sequencing system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021056445A1 (en) * | 2019-09-27 | 2021-04-01 | 深圳华大智造科技有限公司 | Flow cell and biochemical substance reaction device using the flow cell |
-
2018
- 2018-08-16 CN CN201821327067.6U patent/CN208776710U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021056445A1 (en) * | 2019-09-27 | 2021-04-01 | 深圳华大智造科技有限公司 | Flow cell and biochemical substance reaction device using the flow cell |
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Address after: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee after: Shenzhen Huada Zhizao Technology Co., Ltd Address before: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee before: Shenzhen Huada Zhizao Technology Co.,Ltd. |
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| CP01 | Change in the name or title of a patent holder |