CN208868551U - Small sample short distance transports portable liquid nitrogen cup - Google Patents

Abstract

The utility model discloses a kind of small sample short distances to transport portable liquid nitrogen cup, including cup body, cup lid and floating body, cup body is vacuum dual structure, cup body surface is equipped with fluid level scale, cup body side is equipped with several first buckles, first buckle and cup body rotation connection, floating body is hollow closed block, the density of floating body is lower than the density of liquid nitrogen, floating body is equipped with several first grooves, floating body bottom surface is equipped with hollow block corresponding with several first groove locations, first groove and hollow block are integrally formed, hollow block side is equipped with several water inlets, stomata is equipped at the top of cup lid, it is equipped with and matched second groove of cup body in cup lid, the first convex block corresponding with the first groove location is equipped in cup lid, the utility model both can guarantee storage transport temperature, realize -136 degree of whole process or less low temperature guarantee, it can subtract significantly again The volatilizing loss amount of few liquid nitrogen is provided convenience for cryopreservation transport.

Description

Small sample short distance transports portable liquid nitrogen cup

Technical field

The utility model relates to biological sample technical field of transportation, in particular to a kind of small sample short distance transport is portable Liquid nitrogen cup.

Background technique

Traditional experiment room, various cell strain samples are placed in this ultralow temperature ring of liquid nitrogen generally after addition freezes preservative agent Long-term preservation under border.Cell biology shows: -136 degree are the glass transition temperatures of water, the crystallization of water to cell and tissue there is no Obvious to injure, the various biochemical reactions in sample almost stop.Liquid nitrogen environment lower than the critical-temperature is to save in sample carefully The most ecotopia of cytoactive.Freeze-stored cell can be taken out out of liquid nitrogen container and be recovered in 37 degree when needed.Therefore cell When library is designed, often the laboratory GMP and the liquid nitrogen container room of freezing are adjoined, convenience sample resuscitation.Operation is substantially are as follows: liquid nitrogen container Cell sample is taken out, the laboratory GMP pass-through box is passed to.Sample is in 37 degree of water-bath defrostings in the laboratory GMP, finally in clean bench Recovery.But there is also the laboratories GMP and the liquid nitrogen container room of freezing certain distance in reality.The solution provided on the market is big In being mostly, long-range distance then shift sample using liquid nitrogen transfer cask, short distance distance shifts sample using liquid nitrogen transfer cask/transport vehicle Product.For short distance distance transport sample, there is that volumetric constraint, liquid nitrogen consumption are big, transported really using liquid nitrogen transfer cask/vehicle There are many drawbacks such as hidden danger for journey.In addition when recovering experiment, when being removed from liquid nitrogen cell to this section of 37 degree of water-bath recovery cells Between, sample has had disengaged from liquid nitrogen storage environment, operates carry out under normal temperature environment later, sample is in nothing within a certain period of time Low-temperature protection is easy to appear freezing-thawing condition, it is possible to impact to batch sample quality stability.

Summary of the invention

For the above-mentioned prior art, the utility model lies in that provide a kind of small sample short distance transport portable liquid nitrogen cup, Efficiently solve the problems, such as short distance transport cells and guarantee recovery cell before temperature control etc. require.

The technical scheme of the present invention is realized as follows:

A kind of portable liquid nitrogen cup of small sample short distance transport, including cup body, cup lid and floating body, the cup body are vacuum Dual structure, the cup body surface be equipped with fluid level scale, the cup body side be equipped with it is several first buckle, it is described first buckle and The cup body rotation connection, the floating body are hollow closed block, and the density of the floating body is lower than the density of liquid nitrogen, The floating body is equipped with several first grooves, and the floating body bottom surface is equipped with corresponding with several first groove locations Hollow block, first groove and the hollow block are integrally formed, and the hollow block side is equipped with several water inlets, the cup lid Top is equipped with stomata, is equipped in the cup lid and matches with matched second groove of the cup body, the cup lid with the cup body, institute It states and is equipped with the first convex block corresponding with first groove location in cup lid, the cup lid is equipped with matches with first buckle The snap ring of conjunction.

Further, first groove is divided into upper, middle and lower, the middle part protrusion and the top and described For lower part forming position difference for placing cryopreservation tube, the top and the lower part are all larger than the middle part.

Further, the cup body side is equipped with handle.

Further, rubber washer is equipped in second groove.

Further, the cup body and the handle are stainless steel material.

Further, the cup body bottom is equipped with seat cushion.

The utility model has the beneficial effects that: vacuum dual structure, effectively completely cuts off temperature, there is good heat insulating ability Energy；Cup body surface is equipped with fluid level scale, facilitates the pouring volume of viewing liquid nitrogen；Cup body side is equipped with the first buckle, Convenient cup cover lid Tight cup body can effectively reduce after turning on one's side accidentally because of liquid nitrogen cup, and sample spills the risk of loss；Float body swims in liquid nitrogen surface, Space is limited in barycentre steadiness and the first groove, even if being also not easy Fluid Contacting in case of turbulence at cryopreservation tube screw thread mouth Nitrogen efficiently avoids liquid nitrogen and penetrates into cryopreservation tube, to reduce the risk of sample room cross contamination；First groove and hollow block one It is body formed, and hollow block side is equipped with several water inlets, facilitates liquid nitrogen to penetrate into and holds up cryopreservation tube, the final screw thread for guaranteeing cryopreservation tube It is higher than liquid nitrogen liquid level at mouthful；Liquid nitrogen volatilization process can generate pressure, be equipped with stomata at the top of cup lid and be used for pressure release；Second on cup lid Convex block prevents cryopreservation tube from hitting cup lid, and the utility model freezes sample and liquid nitrogen non-direct contact, both can guarantee storage transport Temperature realizes -136 degree of whole process or less low temperature guarantee, and can greatly reduce the volatilizing loss amount of liquid nitrogen, is provided simultaneously with and facilitates reality With, it is safe and reliable, at low cost the advantages that, provide convenience for cryopreservation.Prospect with commercial applications.

Detailed description of the invention

It, below will be to required in embodiment description in order to illustrate more clearly of the technical scheme in the embodiment of the utility model Attached drawing to be used is briefly described, it should be apparent that, the accompanying drawings in the following description is only the preferred reality of the utility model Example is applied, it for those of ordinary skill in the art, without any creative labor, can also be attached according to these Figure obtains other attached drawings.

Fig. 1 is the using renderings that the utility model small sample short distance transports portable liquid nitrogen cup；

Fig. 2 is the cross-sectional view for the cup body that the utility model small sample short distance transports portable liquid nitrogen cup；

Fig. 3 is the front view for the cup body that the utility model small sample short distance transports portable liquid nitrogen cup；

Fig. 4 is the schematic diagram for the cup lid that the utility model small sample short distance transports portable liquid nitrogen cup；

Fig. 5 is the cup lid top view that the utility model small sample short distance transports portable liquid nitrogen cup；

Fig. 6 is the top view for the floating body that the utility model small sample short distance transports portable liquid nitrogen cup.

In figure, 1 cup body, 2 cup lids, 3 floating bodies, 4 fluid level scales, 5 first buckles, 6 first grooves, 7 hollow blocks, 8 water inlets Mouthful, 9 stomatas, 10 second grooves, 11 first convex blocks, 12 snap rings, 13 handles, 14 seat cushions, 15 rubber washers.

Specific embodiment

In order to be best understood from the utility model technology contents, specific embodiment is provided below, and practical to this in conjunction with attached drawing It is novel to be described further.

Referring to Fig. 1 to Fig. 6, a kind of portable liquid nitrogen cup of small sample short distance transport, including cup body 1, cup lid 2 and floating body 3, the cup body 1 is vacuum dual structure, effectively completely cuts off temperature, has good thermal insulation property, and 1 diameter of cup body is 80- 120mm, 1 height of cup body be 80-250mm, 1 surface of cup body be equipped with fluid level scale 4, indicate highest liquid nitrogen liquid level with most Low liquid nitrogen liquid level；1 top-side of cup body is equipped with several first buckles, and Convenient cup cover 2 covers tightly cup body 1, can effectively subtract After turning on one's side accidentally because of liquid nitrogen cup less, sample spills the risk of loss；First buckle and the cup body 1 rotation connection, it is described Floating body 3 is hollow closed block, and 3 material of floating body is polycarbonate or polypropylene, and 3 shape of floating body here is selected Cylindrical body, diameter length 72mm, thick 30mm；The density of the floating body 3 is lower than the density of liquid nitrogen, makes floating body 3 that can float on liquid On nitrogen face, the floating body 3 is equipped with several first grooves 6, and for storing cryopreservation tube, 3-6 the first grooves 6, tool can be selected Body selects 5 the first grooves 6, considers that the diameter of commercial goods cryopreservation tube is generally 10.8-12.5mm, 6 diameter of the first groove About 13-16mm, it is specific to select 6 diameter 14mm of the first groove；3 bottom surface of floating body is equipped with and several first grooves 6 The corresponding hollow block 7 in position, 7 length of hollow block are 5-60mm, specifically select 5mm, diameter and the first groove 6 match；Institute It states the first groove 6 to be integrally formed with the hollow block 7, hollow block 7 is combined with the first groove 6 guarantees that cryopreservation tube can be in always Upright fixed position；7 side of hollow block is equipped with several water inlets 8, and 7 side of hollow block is uniformly provided with 4 water inlets 8, side Just liquid nitrogen, which penetrates into, holds up cryopreservation tube, is higher than liquid nitrogen liquid level at the final screw thread mouth for guaranteeing cryopreservation tube；Gas is equipped at the top of the cup lid 2 Hole 9, liquid nitrogen volatilization process can generate pressure, and 2 top plenum 9 of cup lid is used for pressure release；It is equipped with and the cup body 1 in the cup lid 2 Matched second groove 10, the cup lid 2 are matched with the cup body 1, are equipped with and 6 position of the first groove in the cup lid 2 Corresponding first convex block 11, the first convex block 11 on cup lid 2 prevent cryopreservation tube from hitting cup lid 2, and the cup lid 2 is equipped with and institute State the first snap ring 12 being clasped.

Specifically, first groove 6 divides for upper, middle and lower, the middle part protrusion and the top and described For placing cryopreservation tube, the top and the lower part are all larger than the middle part, can make cryopreservation tube lower part forming position difference Screw thread mouth is stuck in top, prevents cryopreservation tube from falling off into the first groove 6, prevents from contacting liquid nitrogen at screw thread mouth, avoids liquid nitrogen infiltration Enter cryopreservation tube, to reduce the risk of sample room cross contamination.

Specifically, 1 side of cup body is equipped with handle 13, it is convenient for taking and placing.

Specifically, rubber washer 15 is equipped in second groove 10, it is convenient closed with cup body 1.

Specifically, the cup body 1 and the handle 13 are stainless steel material, non-corrosive is not easy to be corroded by liquid nitrogen.

Specifically, 1 bottom of cup body is equipped with seat cushion 14, bottom of a cup is protected.

Working principle: in use, opening cup lid 2, it is just put into float body, is poured into enough liquid nitrogen, so that the height of float body Degree is between the highest liquid nitrogen liquid level and minimum liquid nitrogen liquid level of 1 surface level scale 4 of cup body, with hemostasis out of liquid nitrogen container Pincers take out cryopreservation tube, direct plunge into the first groove 6 of float body, cryopreservation tube at this time floats on liquid nitrogen, but screw thread mouth Locating chaotropic nitrogen, there are certain distances, cover cup lid 2, the snap ring 12 of cup lid 2 is sticked into the first card by the first buckle of rotation cup body 1 Button, covers tightly cup lid 2, then takes liquid nitrogen cup and be transferred to by laboratory water bath pot through pass-through box, after laboratory technician is ready Start resuscitation, is removed from liquid nitrogen cell to recovery cell this period, whole process is maintained at -136 and spends hereinafter, avoiding cold Freezing temperature recovery of the sample in transfer process, the water in freeze-stored cell not will form crystallization, and eucaryotic cell structure not will receive destruction, And cryopreservation tube does not leak into liquid nitrogen, to guarantee the quality of freeze-stored cell to greatest extent, keeps Cell viability after recovery higher.

The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this Within the spirit and principle of utility model, any modification, equivalent replacement, improvement and so on should be included in the utility model Protection scope within.

Claims (6)

1. a kind of small sample short distance transports portable liquid nitrogen cup, which is characterized in that described including cup body, cup lid and floating body Cup body is vacuum dual structure, and the cup body surface is equipped with fluid level scale, and the cup body side is equipped with several first buckles, described First buckle and cup body rotation connection, the floating body are hollow closed block, and the density of the floating body is lower than The density of liquid nitrogen, the floating body are equipped with several first grooves, and the floating body bottom surface is equipped with and several first grooves The corresponding hollow block in position, first groove and the hollow block are integrally formed, the hollow block side be equipped with it is several into The mouth of a river is equipped with stomata at the top of the cup lid, is equipped in the cup lid and matched second groove of the cup body, the cup lid and institute State cup body matching, be equipped with the first convex block corresponding with first groove location in the cup lid, the cup lid be equipped with Described first snap ring being clasped.

2. a kind of small sample short distance according to claim 1 transports portable liquid nitrogen cup, which is characterized in that described first Groove is divided into upper, middle and lower, the middle part protrusion and the top and the lower part forming position difference and freezes for placing Pipe is deposited, the top and the lower part are all larger than the middle part.

3. a kind of small sample short distance according to claim 1 transports portable liquid nitrogen cup, which is characterized in that the cup body Side is equipped with handle.

4. a kind of small sample short distance according to claim 1 transports portable liquid nitrogen cup, which is characterized in that described second Rubber washer is equipped in groove.

5. a kind of small sample short distance according to claim 3 transports portable liquid nitrogen cup, which is characterized in that the cup body It is stainless steel material with the handle.

6. a kind of small sample short distance according to claim 1 transports portable liquid nitrogen cup, which is characterized in that the cup body Bottom is equipped with seat cushion.