CN210945553U - Ice box for second-generation sequencing - Google Patents

Abstract

The utility model discloses an ice box used in the second generation sequencing, which comprises an ice box main body, a reagent taking and placing moving device and a plastic rubber stick, wherein the middle parts of the two sides of the inner part of the ice box main body are embedded and connected with the reagent taking and placing moving device, the inner part of the ice box main body is fixedly connected with a roller, the inner part of the ice box main body is movably connected with a rubber ball, the front surface of the reagent taking and placing moving device is fixedly connected with a handle, the middle parts of the two sides of the reagent taking and placing moving device are embedded and connected with a roller moving groove, the top of the reagent taking and placing moving device is fixedly connected with a test tube storage groove, the test tube storage groove embodies the real-time property of the ice box, the reagent taking and placing moving device embodies the taking high efficiency of the ice box, a foaming plate embodies the temperature stability of the ice box, a water collecting groove embodies the cleanness of the ice box, embedded visual glass, is suitable for biology use and has wide development prospect in the future.

Description

Ice box for second-generation sequencing

Technical Field

The utility model relates to the field of biological exploration, in particular to an ice box used in the second generation sequencing.

Background

The second-generation sequencing technology greatly reduces the sequencing cost, greatly improves the sequencing speed, maintains high accuracy, and needs 3 years for completing the sequencing of a human genome in the past, while the second-generation sequencing technology only needs 1 week, but is much shorter than the first-generation sequencing technology in the sequence reading aspect.

The secondary sequencing is very much to test the safety of reagents or cultured cells during sequencing, once some reagents are polluted or different reagents are mixed and melted when the secondary sequencing is stored in an ice box, great errors can be caused in sequencing, the constant temperature capability of the secondary sequencing device is not strong at present, the secondary sequencing device is optimal for testing and culturing cells and reagents at low temperature, and the conventional device is easy to change along with the external temperature.

Therefore, how to design an ice box for use in next generation sequencing becomes a problem to be solved currently.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an ice box for in two take place ofs sequencing to the ice box functional insufficiency, the protectiveness that are arranged in two take place ofs sequencing that provide in solving above-mentioned background art are not strong and get the general problem of putting the nature.

In order to achieve the above object, the utility model provides a following technical scheme: an ice box for second-generation sequencing comprises an ice box main body, a reagent taking and placing moving device and a plastic rubber rod, wherein the top of the ice box main body is movably connected with an ice box cover, the middle parts of two sides in the ice box main body are connected with the reagent taking and placing moving device in an embedding manner, the front of the reagent taking and placing moving device is fixedly connected with a handle, the middle parts of two sides of the reagent taking and placing moving device are connected with roller moving grooves in an embedding manner, the top of the reagent taking and placing moving device is fixedly connected with a test tube storage groove, the other end of the reagent taking and placing moving device is connected with a groove in an embedding manner, one side of the reagent taking and placing moving device is connected with a convex block in an embedding manner, the inner part of the ice box main body is fixedly connected with rollers, the bottom in the ice box main body is fixedly connected with a cushioning spring, the inner part, and plastic rubber rods are embedded and connected to two sides of the ice box main body.

Preferably, the top of the refrigerator cover is fixedly connected with embedded visual glass.

Preferably, two sides of the interior of the ice box main body are fixedly connected with PE plastic shells, and the interior of the PE plastic shells is fixedly connected with a foaming plate.

Preferably, a PCR reaction plate is embedded and connected to the bottom of the ice box main body, and a reaction port is fixedly connected to the top of the PCR reaction plate.

Preferably, the front bottom end of the ice box main body is fixedly connected with a water collecting tank, and the top of the water collecting tank is fixedly connected with a filter screen.

Preferably, the reagent taking and placing moving device is composed of roller moving grooves on two sides, a test tube storage groove at the top of the reagent taking and placing moving device, a groove at the other end of the reagent taking and placing moving device, a protruding block on one side of the reagent taking and placing moving device and rollers inside the ice box main body.

Preferably, the cushioning protection mechanism is composed of a cushioning spring inside the ice box main body, a rubber ball inside the ice box main body, cylindrical grooves on two sides of the ice box main body and plastic rubber rods on two sides of the ice box main body.

Compared with the prior art, the beneficial effects of the utility model are that:

1. this kind of an ice box for in two take place ofs sequencing opens the ice box lid, alright put into the material that needs deposit both sides reagent and get the test tube storage tank of putting mobile device, can also select to carry and carry the arbitrary handle in both sides, and pulling force makes gyro wheel and gyro wheel movable slot take place to slide, and the device is pulled out then lays, then alright push away the handle inwards, until protruding piece and recess coincidence can.

2. This kind of an ice box for in two take place ofs sequencing, when the ice box received the external force of below, the cushioning spring maximize fall certain impact force buffering, the rubber ball is further absorbs the impact force and disperses, the loss that brings external force falls to minimumly, when both sides received external force, plasticity rubber stick protected the device, if surpass the limit, just can take place deformation with external force dispersion, maximize protection device.

Drawings

Fig. 1 is an overall schematic view of the present invention;

FIG. 2 is a schematic cross-sectional view of an ice box in the second generation sequencing of the present invention;

fig. 3 is a schematic sectional view of the storage device of the present invention;

fig. 4 is a schematic top view of the storage device of the present invention.

In the figure: 1. ice box main part, 2, reagent are got and are put mobile device, 201, handle, 202, gyro wheel activity groove, 203, test tube storage tank, 204, gyro wheel, 205, recess, 206, protruding piece, 3, the ice-box lid, 4, embedded visual glass, 5, PCR reaction plate, 501, reaction mouth, 6, water catch bowl, 601, filter screen, 7, plasticity rubber stick, 8, bradyseism spring, 9, rubber ball, 10, cylindricality recess, 11, foaming board, 12, PE plastic housing.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative work belong to the protection scope of the present invention based on the embodiments of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: an ice box used in the second generation sequencing comprises an ice box main body 1, a reagent taking and placing moving device 2 and a plastic rubber rod 7, wherein the top of the ice box main body 1 is movably connected with an ice box cover 3, the middle parts of the two sides in the ice box main body 1 are connected with the reagent taking and placing moving device 2 in an embedding way, the front of the reagent taking and placing moving device 2 is fixedly connected with a handle 201, the middle parts of the two sides of the reagent taking and placing moving device 2 are connected with a roller moving groove 202 in an embedding way, the top of the reagent taking and placing moving device 2 is fixedly connected with a test tube storage groove 203, the other end of the reagent taking and placing moving device 2 is connected with a groove 205 in an embedding way, one side of the reagent taking and placing moving device 2 is connected with a convex block 206 in an embedding way, the roller 204 is fixedly connected in the ice box main body 1, the, the two sides of the ice box main body 1 are fixedly connected with cylindrical grooves 10, and the two sides of the ice box main body 1 are embedded and connected with plastic rubber rods 7.

Preferably, the top of the ice box cover 3 is fixedly connected with the embedded visual glass 4, the safety of reagents or cultured cells is greatly tested during sequencing by secondary sequencing, once the ice box is stored, certain contaminated or different reagents are mixed and melted, great sequencing errors can be caused, the embedded visual glass 4 is arranged, the condition of the ice box can be observed, once the contamination or abnormal phenomenon occurs, the treatment can be immediately carried out, and the reagents in the whole device are prevented from being contaminated.

Preferably, the inside both sides fixedly connected with PE plastic housing 12 of ice box main part 1, the inside fixedly connected with foaming board 11 of PE plastic housing 12, the constant temperature ability of secondary sequencing device is not strong now, and the experiment is optimal with cell and the reagent to the cultivation in low temperature, present device is very easy along with external temperature variation, set up foaming board 11, the ice-box is put into the refrigerator, the ice crystal material in PE plastic housing 12 freezes stores up coldly, the excellent heat preservation effect that foaming board 11 possessed, mutual interaction between separation ambient temperature and the device temperature that can be fine, make the reaction possess good environment.

Preferably, the bottom embedding of ice box main part 1 is connected with PCR reaction plate 5, the top fixed connection reaction port 501 of PCR reaction plate 5, in present second generation sequencing, the cell of cultivateing and reaction plate are two independent units, it is very not convenient to make the operation during the experiment, set up PCR reaction plate 5, make the experiment can accomplish in same device, get reagent and put reagent or cell that mobile device 2 was chosen to carry out the sequencing needs in reagent, get reagent and put mobile device 2, outwards pull, mix reagent and pour reaction port 501 into, observe the reaction, the record data.

Preferably, the preceding bottom fixedly connected with water catch bowl 6 of ice box main part 1, the top fixedly connected with filter screen 601 of water catch bowl 6, when the laboratory is tested, ice box contacts the slow interaction between the ambient temperature, must have the drop of water that melts and fall on the laboratory bench, and such experiment of second generation sequencing is very high to sanitary requirement, then water is infected with bacterium and pollutant very easily, set up basin 6, the drop of water all can enter into the inslot, filter screen 601 can filter the absorbent pollutant of drop of water, the holistic cleanliness factor of holding device, also make things convenient for the clearance after the experiment.

Preferably, the reagent taking and placing moving device 2 is composed of roller moving grooves 202 on two sides, a test tube storage groove 203 on the top of the reagent taking and placing moving device 2, a groove 205 on the other end of the reagent taking and placing moving device 2, a convex block 206 on one side of the reagent taking and placing moving device 2, and rollers 204 inside the ice box main body 1, the equipment such as an ice box in the second-generation sequencing is not mature enough, the storage and the taking of reagents are very complicated, the reagent taking and placing moving device 2 is arranged, when the reagents or cultured cells need to be stored, the ice box cover 3 is opened, the substances needing to be stored can be placed in the test tube storage grooves 203 of the reagent taking and placing moving devices 2 on two sides, and when a large amount of storage is not needed, any handle 201 on two sides can be selected to be lifted, the rollers 204 slide with the roller moving grooves 202 due to the pulling force, the device is pulled out, and then any reagent, then alright push handle 201 inwards, until protruding piece 206 and recess 205 coincidence can, just can not take place device's skew, if when needing the experiment, just can be with the handle 201 of both sides all outwards draw, pulling force makes gyro wheel 204 and gyro wheel movable slot 202 take place to slide, the reagent of both sides is got and is put mobile device 2 and is pulled out, because gyro wheel movable slot 202 is close to laminating with gyro wheel 204, so its both frictional force can make reagent under most circumstances get and put mobile device 2 and fix, take out required article alright with direct sequencing on below PCR reaction plate 5 at test tube storage tank 203, still make the device have certain dismantlement, convenient washing.

Preferably, the cushioning spring 8 in the ice box main body 1, the rubber ball 9 in the ice box main body 1, the cylindrical grooves 10 on both sides of the ice box main body 1 and the plastic rubber bars 7 on both sides of the ice box main body 1 jointly form a cushioning protection mechanism, most ice boxes in sequencing do not have any protection measure, experimenters can bring cultured cells to a torch if carelessness occurs during taking and placing, the cushioning protection mechanism is arranged, when the ice boxes are subjected to external force below, the cushioning spring 8 has the characteristic of maximally cushioning a certain impact force, the impact force is further absorbed and dispersed through the rubber ball, the loss caused by the external force is reduced to the minimum, when the external force is applied to both sides, the plastic rubber bars 7 protect the device at the maximum cushioning limit, if the limit is exceeded, the deformation can occur to disperse the external force, the device is maximally protected, the plasticity rubber stick 7 is installed outside the device, is very easy to be replaced by low temperature and normal temperature, and the rubber characteristic will be gradually weakened after a long time, and at this time, the plasticity rubber stick 7 can be taken out from the cylindrical groove 10, and the plasticity rubber stick 7 which is replaced by new one is installed in the cylindrical groove 10, so that the safety of the ice box is ensured.

The working principle is as follows: firstly, the embedded visual glass 4 is arranged, so that the condition of the ice box can be observed, once pollution or abnormal phenomenon occurs, the ice box can be immediately treated, and the reagent in the whole device is prevented from being polluted;

then, a foaming plate 11 is arranged, the ice box is placed in a refrigerator, ice crystal substances in the PE plastic shell 12 are frozen and stored cold, the foaming plate 11 has excellent heat preservation effect, the interaction between the external temperature and the device temperature can be well blocked, and the reaction has a good environment;

then, when reagent or cultured cells need to be stored, the ice box cover 3 is opened, substances needing to be stored can be placed into the test tube storage grooves 203 of the reagent taking and placing moving devices 2 on the two sides, and when a large amount of the substances do not need to be stored, any handle 201 on the two sides can be selectively lifted, the roller 204 and the roller movable groove 202 slide by pulling force, the device is pulled out, then any reagent taking and placing moving device 2 is selected for placing, then the handle 201 can be pushed inwards until the protruding block 206 is overlapped with the groove 205, the device can not shift, if an experiment is needed, all the handles 201 on the two sides can be pulled outwards, the roller 204 and the roller movable groove 202 slide by pulling force, the reagent taking and placing moving devices 2 on the two sides are pulled out, because the roller movable groove 202 is closely attached to the roller 204, the friction force between the two can fix the reagent taking and placing moving devices 2 under the condition that most of the two devices are closely attached, the needed articles are taken out from the test tube storage tank 203, so that sequencing can be directly carried out on the PCR reaction plate below the test tube storage tank, and the device has certain detachability and is convenient to clean;

next, a cushioning protection mechanism is arranged, when the ice box is subjected to external force below, certain impact force can be maximally buffered by the cushioning spring 8, then the impact force is further absorbed and dispersed by the rubber ball, loss caused by the external force is minimized, when the ice box is subjected to the force from two sides, the plastic rubber rod 7 can protect the ice box at the maximum cushioning limit, if the impact force exceeds the limit, the plastic rubber rod 7 can deform to disperse the external force, the device is maximally protected, the plastic rubber rod 7 is arranged outside the ice box and is easily replaced by low temperature and normal temperature, the rubber characteristic can be gradually weakened after a long time, and in this time, the plastic rubber rod 7 can be taken out of the cylindrical groove 10, a new plastic rubber rod 7 is arranged in the cylindrical groove 10, and the safety of the ice box is ensured;

finally, set up basin 6, the drop of water all can enter the inslot, and filter screen 601 can filter the absorbent pollutant of drop of water, the holistic cleanliness factor of retention device, also convenient clearance after the experiment, fine embodiment take high efficiency and the practicality of the ice chest in the second generation sequencing, this kind of theory of operation that is arranged in the ice chest of second generation sequencing.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. The utility model provides an ice chest for in next generation sequencing, includes that ice chest main part (1), reagent are got and are put mobile device (2) and plasticity rubber stick (7), its characterized in that: the reagent taking and placing device comprises an ice box main body (1), an ice box cover (3) is movably connected to the top of the ice box main body (1), reagent taking and placing moving devices (2) are connected to the middle parts of two sides of the inner portion of the ice box main body (1) in an embedded mode, a handle (201) is fixedly connected to the front of each reagent taking and placing moving device (2), roller moving grooves (202) are connected to the middle parts of two sides of each reagent taking and placing moving device (2) in an embedded mode, test tube storage grooves (203) are fixedly connected to the top of each reagent taking and placing moving device (2), grooves (205) are connected to the other ends of the reagent taking and placing moving devices (2) in an embedded mode, protruding blocks (206) are connected to one side of each reagent taking and placing moving device (2) in an embedded mode, rollers (204) are fixedly connected to the inner portion of the ice box main body (1), a cushioning spring (, the ice box is characterized in that cylindrical grooves (10) are fixedly connected to two sides of the ice box body (1), and plastic rubber rods (7) are embedded and connected to two sides of the ice box body (1).

2. An ice cassette for use in next generation sequencing according to claim 1, wherein: the top of the refrigerator cover (3) is fixedly connected with embedded visual glass (4).

3. An ice cassette for use in next generation sequencing according to claim 1, wherein: the ice box is characterized in that PE plastic shells (12) are fixedly connected to two sides of the interior of the ice box main body (1), and foaming plates (11) are fixedly connected to the interior of the PE plastic shells (12).

4. An ice cassette for use in next generation sequencing according to claim 1, wherein: the bottom of the ice box main body (1) is embedded and connected with a PCR reaction plate (5), and the top of the PCR reaction plate (5) is fixedly connected with a reaction port (501).

5. An ice cassette for use in next generation sequencing according to claim 1, wherein: the ice box is characterized in that the front bottom end of the ice box main body (1) is fixedly connected with a water collecting tank (6), and the top of the water collecting tank (6) is fixedly connected with a filter screen (601).

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