CN208860743U - Spectrometer suitable for the lossless fluorescence detection of cellular level - Google Patents
Spectrometer suitable for the lossless fluorescence detection of cellular level Download PDFInfo
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- CN208860743U CN208860743U CN201821599702.6U CN201821599702U CN208860743U CN 208860743 U CN208860743 U CN 208860743U CN 201821599702 U CN201821599702 U CN 201821599702U CN 208860743 U CN208860743 U CN 208860743U
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- 230000001413 cellular effect Effects 0.000 title claims abstract description 10
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 9
- 239000000523 sample Substances 0.000 claims abstract description 62
- 238000004458 analytical method Methods 0.000 claims abstract description 22
- 230000005284 excitation Effects 0.000 claims abstract description 21
- 238000002189 fluorescence spectrum Methods 0.000 claims abstract description 9
- 239000003550 marker Substances 0.000 claims description 5
- 230000003287 optical effect Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 12
- 238000012360 testing method Methods 0.000 abstract description 4
- 206010028980 Neoplasm Diseases 0.000 abstract description 3
- 201000011510 cancer Diseases 0.000 abstract description 3
- 238000003384 imaging method Methods 0.000 abstract description 3
- 238000012216 screening Methods 0.000 abstract description 2
- 238000010183 spectrum analysis Methods 0.000 abstract description 2
- 230000003834 intracellular effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002548 cytokinetic effect Effects 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model discloses a kind of spectrometers suitable for the lossless fluorescence detection of cellular level, including exciting light sources, exciting light sources lower end is connected with excitation light probe, light probe is excited to connect exciting light position control system by exciting light probe fixing device, accurate digital control motor is provided in exciting light position control system, excitation light probe issues the sample cell being provided with for placing cell sample, is fluorescence spectrum collection and analysis system below sample cell.Sample cell is arranged in intermediate sample cell position control system hollow, surrounding is fixed.The utility model can be used for unicellular/subcellular fluorescence light analysis, it can be achieved that phosphorescent lifetime test, can realize that surveying fluorescence intensity changes with time within the scope of millisecond to hour time scale；Complete stable state spectrum analysis on unicellular/subcellular fluorescent microscopic imaging, microcell spectrofluorimetry and the direction X-Y, cancer detection and screening to early stage have biggish directive significance.
Description
Technical field
The utility model relates to optical technology biological field applied technical field.
Background technique
In recent years, gradually go deep into cell physiological research, scientists discovery, even same inhereditary material,
Same surrounding enviroment, these cells still can develop towards different directions, can also generate different functions sometimes.It is single thin
Born of the same parents seem identical, but its chemical component and stress reaction are very different, and the variation of individual cells is also relate to cancer, mind
Through major diseases such as diseases.The unicellular organism process of plant and animal is also far from disclosing completely.Therefore single cell analysis
It is the important means for understanding and decoding the life process of many keys.But single celled size is very small, the chemistry included
Ingredient is also ultramicron, and current analysis method Western blot, then needs by being crushed millions of cells
The detection of intracellular biochemical index is realized, so traditional analysis is not enough to find the difference in individual cells level.No
Only in this way, conventional method is mostly to pinpoint detection, real-time monitoring cannot achieve.
Current Fluorescence Spectrometer is commonly used to the detection of macroscopical sample, there are no be directed to cellular level accurate detection,
Main cause is that the exciting light probe diameter of common spectrometer is too thick, because the diameter of general cell is 5-10 microns, and is popped one's head in
Diameter is usually tens millimeters to tens centimetres, can not accurately be excited to cell, in addition, the signal due to cell is more micro-
Weak, laser power is difficult to control, power too it is weak can not in activated cell signal, power it is too strong then to cell damage.
Summary of the invention
The technical problem to be solved by the utility model is to provide one kind for unicellular/subcellular fluorescence light analysis, can
Realize the spectrometer of the lossless fluorescence detection of cellular level of phosphorescent lifetime test.
A kind of spectrometer suitable for the lossless fluorescence detection of cellular level includes that light source generates emitter, micro-nano detection
Device, micro-nano detector controling adjustment device, cell sample pond, cell sample pond controling adjustment device, at spectrographic detection analysis
Manage device.
A kind of spectrometer suitable for the lossless fluorescence detection of cellular level, including exciting light sources, exciting light sources lower end
It is connected with excitation light probe, excitation light probe connects exciting light position control system, excitation by exciting light probe fixing device
Accurate digital control motor is provided in optical position control system, excitation light probe issues the sample being provided with for placing cell sample
Pond, fluorescent marker is provided in cell sample, is that fluorescence spectrum is collected and analysis system below sample cell.
Sample cell is arranged in intermediate sample cell position control system hollow, surrounding is fixed.
The utility model has the advantages that the utility model can be used for it is unicellular/subcellular fluorescence light analysis, it can be achieved that phosphorescent lifetime test,
It can realize that surveying fluorescence intensity changes with time within the scope of millisecond to hour time scale；Unicellular/subcellular fluorescence microscopy
Complete stable state spectrum analysis on imaging, microcell spectrofluorimetry and the direction X-Y, to the cancer detection and screening of early stage
With biggish directive significance.
Detailed description of the invention:
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is another structural schematic diagram of the utility model.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawing.
A kind of spectrometer suitable for the lossless fluorescence detection of cellular level, including exciting light sources 1, under exciting light sources 1
End is connected with excitation light probe 2, and excitation light probe 2 connects exciting light position control system by exciting light probe fixing device 3.2
System 3 is provided with accurate digital control motor 3.1 in exciting light position control system 3, and excitation light probe 2, which issues, to be provided with for placing
The sample cell 4 of cell sample is provided with fluorescent marker 9 in cell sample 8, collected below sample cell 4 for fluorescence spectrum and point
Analysis system 7.
Exciting light probe fixing device 3.2 can drive exciting light sources 1 and the excitation light probe 2 of connection along exciting light
Track in position control system 3 moves up and down.
Sample cell 4 is arranged in intermediate sample cell position control system 5 hollow, surrounding is fixed.Sample cell 4 is for placing
The cell sample of required detection.Sample cell position control system 5 is used for precise positioning cell, minimal adjustment precision 10um.
Exciting light sources 1: the excitation of any one single wavelength of generation wavelength within the scope of 230-850nm can be excited
Light.
It is two kinds that excitation light probe 2, which is divided to: big 12 head sizes of excitation light probe of one kind are used within the scope of 100-900nm
In detection subcellsular level, it can stretch to inside cell and nucleus and be excited；Another small 2 head ruler of excitation light probe
It is very little within the scope of 1-10um, for detecting entire individual cell level,
3.1 minimal adjustment precision 30nm of accurate digital control motor.
The minimal adjustment precision 10um of sample cell position control system 5, can be adjusted, stroke is positive and negative in X, Y, Z axis
13mm。
When containing fluorescent marker 9 in cell sample 8, exciting light sources 1 will emit the exciting light of corresponding wavelength, should
Excitation imported into the outside of cell sample 8 by excitation light probe or is pierced into the inside of cell sample 8, will excite fluorescent marker
9, which generate transmitting light, forms fluorescence signal.
Fluorescence spectrum collects the hypersensitive fluorescence signal that individual cells level can be collected into analysis system 7, Neng Goushou
The minimum photon numbers collected are 50, and the smallest wave crest bandwidth is 5nm.
Fluorescence spectrum collects that (including fluorescence is strong and weak, fluorescence luminous point point by the fluorescence signal that is collected into analysis system 7
Cloth, fluorescence luminous point change over time) it is analyzed, can be used for: 1) by laser probe, 2) carry out intracellular phosphorescence point
Analysis realizes that phosphorescent lifetime is tested, 3) intracellular Fluorescence microscopic image analysis.
Fluorescence spectrum collects that (including fluorescence is strong and weak, fluorescence luminous point point by the fluorescence signal that is collected into analysis system 7
Cloth, fluorescence luminous point change over time) it is analyzed, it can be used for:
1) entire cell phosphorescence analysis is carried out by big laser probe (12), realizes phosphorescent lifetime test；
2) entire cytokinetic analysis is carried out by big laser probe 12；
3) entire luminescence of cell efficiency analysis；
4) complete stable state spectrum point on entire cell fluorescence micro-imaging, microcell spectrofluorimetry and the direction X-Y
Analysis.
Light source required for the utility model is generated by exciting light sources 1 is by micro-nano detection probe (by micro-nano
Detection probe controling adjustment device is accurately positioned detector) cell to be detected on Lai Jifa sample cell 4, the result quilt of generation
Fluorescence spectrum, which is collected, receives analysis processing with analysis system 7, obtains required result.
1 energy generation wavelength of exciting light sources sweeps excitation within the scope of 230-850nm and retouches light, can also pass through built-in monochromator
Any one wavelength of selected wavelength within the scope of 230-850nm generates monochromatic excitation light.
Exciting light sources 1 can select model M365F1, M490F3, M530F2 equal-specification.
It is known product, model RMA-PMS-400A that fluorescence spectrum, which is collected with analysis system 7,.
Claims (2)
1. a kind of spectrometer suitable for the lossless fluorescence detection of cellular level, it is characterised in that: including exciting light sources (1), swash
Illuminating source (1) lower end is connected with excitation light probe, and excitation light probe passes through exciting light probe fixing device (3.2) connection excitation
Optical position control system (3) is provided with accurate digital control motor (3.1) in exciting light position control system (3), excites light probe
(2) sample cell (4) being provided with for placing cell sample (8) is issued, is provided with fluorescent marker (9) in cell sample (8),
It is that fluorescence spectrum is collected and analysis system (7) below sample cell (4).
2. the spectrometer according to claim 1 suitable for the lossless fluorescence detection of cellular level, it is characterised in that: the sample
Product pond (4) is arranged in intermediate sample cell position control system (5) hollow, surrounding is fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821599702.6U CN208860743U (en) | 2018-09-29 | 2018-09-29 | Spectrometer suitable for the lossless fluorescence detection of cellular level |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821599702.6U CN208860743U (en) | 2018-09-29 | 2018-09-29 | Spectrometer suitable for the lossless fluorescence detection of cellular level |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208860743U true CN208860743U (en) | 2019-05-14 |
Family
ID=66419814
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821599702.6U Withdrawn - After Issue CN208860743U (en) | 2018-09-29 | 2018-09-29 | Spectrometer suitable for the lossless fluorescence detection of cellular level |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109164080A (en) * | 2018-09-29 | 2019-01-08 | 江苏瑞明生物科技有限公司 | Suitable for the lossless fluorescence detection spectrometer of cellular level |
-
2018
- 2018-09-29 CN CN201821599702.6U patent/CN208860743U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109164080A (en) * | 2018-09-29 | 2019-01-08 | 江苏瑞明生物科技有限公司 | Suitable for the lossless fluorescence detection spectrometer of cellular level |
| CN109164080B (en) * | 2018-09-29 | 2024-06-14 | 江苏瑞明生物科技有限公司 | Spectrum suitable for cell level nondestructive fluorescence detection |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20190514 Effective date of abandoning: 20240614 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20190514 Effective date of abandoning: 20240614 |
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| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |