CN208293022U - A kind of culture apparatus for repeating to adsorb T cell - Google Patents
A kind of culture apparatus for repeating to adsorb T cell Download PDFInfo
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- CN208293022U CN208293022U CN201820503348.6U CN201820503348U CN208293022U CN 208293022 U CN208293022 U CN 208293022U CN 201820503348 U CN201820503348 U CN 201820503348U CN 208293022 U CN208293022 U CN 208293022U
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- culture
- culture tank
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- top cover
- culturing rack
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Abstract
The utility model provides a kind of culture apparatus for repeating to adsorb T cell, including pedestal, culturing rack and top cover, top cover is covered forms the confined space isolation external world on culturing rack, the culturing rack includes the L-type end plate at both ends, by transverse direction between two end plates, longitudinal partition is separated into multiple culture spaces, in culture space on two sidewalls recessing to engage culture tank, culture tank from top to bottom successively includes the first culture tank, second culture tank, wherein the first culture tank, by being cased with, the pin shaft of torsional spring is hinged and articulated position is laid with rubber elastic membrane for second culture tank two sides cell wall and bottom wall, both ends are made of elastic rubber wall, and strainer is set on bottom wall, closure door is set below strainer, first culture tank, two cell wall top is provided with liquid-leaking nozzle, electromagnet is provided in top cover and pedestal, the separation and increment of present apparatus integration progress T cell, The loss of T cell is reduced, repeatability absorption T cell improves separation quantity, replaceable culture tank, validity period limit for length.
Description
Technical field
The utility model relates to a kind of culture apparatus for repeating to adsorb T cell.
Background technique
T cell is segmented into many types, such as cytotoxic T cell according to function and surface marker, T helper cell, adjust/
Inhibit T cell etc., with the development of biological therapy clinical application, T cell is more and more wider in the research and application of medical field
General, T cell is generally in-vitro multiplication, need to first separate and be proliferated again, and it is carrier that this uses the utensils such as culture dish in the process, and is being cultivated
In the process, the replacement for needing often to carry out utensil, causes the loss of T cell during replacement, improve cost, can further make
Biggish error is generated at experimental result.
Utility model content
In view of this, reducing T cell the purpose of the utility model is to provide a kind of culture apparatus for repeating to adsorb T cell
Loss, integrated Isolation and proliferation T cell, other this culture apparatus validity period limit for length.
In order to solve the above technical problems, the technical solution of the utility model is:
A kind of culture apparatus for repeating to adsorb T cell, including pedestal, culturing rack and top cover, top cover cover the shape on culturing rack
Completely cut off the external world at confined space, the culturing rack includes the L-type end plate at both ends, is divided between two end plates by lateral, longitudinal partition
Multiple culture spaces are divided into, to engage culture tank, culture tank from top to bottom successively includes for recessing on two sidewalls in culture space
First culture tank, the second culture tank, wherein the first culture tank, the second culture tank two sides cell wall and bottom wall are by being cased with torsional spring
Pin shaft is hinged and articulated position is laid with rubber elastic membrane, and both ends are made of elastic rubber wall, and strainer is set on bottom wall, under strainer
Side sets closure door, and two cell wall top of the first culture tank is provided with liquid-leaking nozzle, is provided with electromagnet in top cover and pedestal.
This culture apparatus is by installing electromagnet in top cover, pedestal, using the magnetic force of pedestal electromagnet first by culture tank
Interior magnetic bead concentrates on bottom wall, and the closure door in the first culture tank is opened, and mixed liquor falls into the second culture tank through magnetic bead, further
Magnetic bead is contacted, the separation quantity of T cell is improved, the second culture tank can continue with magnetic bead absorption T cell, and dual absorption increases T
The separation quantity of cell, the electromagnet in top cover help the second culture tank row by the magnetic bead in the second culture tank of magnetic-adsorption
Waste liquid out, in order to carry out the proliferation of T cell, the liquid-leaking nozzle of the first culture tank can leak into the second culture tank through culture solution, carry out T
The proliferation of cell, and two cell wall elastic hinge joint bottom wall of culture tank, end is elastic rubber wall, compressible, convenient for culture tank and training
The assembling and replacement of frame are supported, service life is improved, the rubber membrane of articulated position can prevent leakage.
Further, the pedestal upper limb sets card slot, engages culturing rack.
Further, the top cover two sidewalls are provided with eaves edge, and eaves is bonded along outside with card slot, and inside is bonded culturing rack, lead to
It crosses top cover to cover on culturing rack, on the one hand makes the culture space in culturing rack relatively independent, another aspect top cover, culturing rack and bottom
Seat constitutes closed culture space, reduces extraneous interference.
Further, electromagnet is set at the top of the top cover.
Further, the pedestal side is provided with liquid outlet, and is connected to each culture space by pipeline, useless convenient for discharge
Liquid.
The utility model has the beneficial effects that this culture dish repeats adsorbing separation T cell using same covering device, it is thin to improve T
The separation quantity and Multiplying culture T cell of born of the same parents, and the culture tank of elastic hinge joint is convenient for assembling and replacement, expanded service life.
Detailed description of the invention
Fig. 1: the structure chart of the utility model；
Fig. 2: the first, second culture tank structure chart of the utility model；
Fig. 3: the culturing rack structure chart of the utility model；
Fig. 4: the outline drawing of the utility model；
In figure: 1, pedestal；1.1, electromagnet；1.2, liquid outlet；1.3, card slot；2, top cover；2.1, eaves edge；3, culturing rack；
3.1, partition；3.2, end plate；4, the first culture tank；4.1, liquid-leaking nozzle；4.2, rubber membrane；4.3, pin shaft；4.4, strainer；4.5, it seals
Door；5, the second culture tank.
Specific embodiment
Below in conjunction with attached drawing, specific embodiment of the present utility model is described in further detail, so that the utility model skill
Art scheme is more readily understood and grasps.
Fig. 1 illustrates a kind of specific structure of culture apparatus for repeating absorption T cell, including pedestal 1, culturing rack 3 and top
Lid 2 is provided with electromagnet 1.1 in the top of top cover 2 and pedestal 1, utilizes the magnetic in the magnetic-adsorption culture tank of electromagnet 1.1
Pearl, to separate T cell, 2 sets of top cover form the confined space isolation external world on culturing rack 3, and pedestal 1 and culturing rack 3 can be by poly-
Polypropylene injection molding, 1 upper limb of pedestal set card slot 1.3, engage culturing rack 3, and the culturing rack 3 includes the L-type end plate 3.2 at both ends,
Multiple culture spaces are separated by partition 3.1 laterally, longitudinal between two end plates 3.2, as shown in figure 3, using in the present embodiment
Vertical 6 partitions 3.1 of one cross five are divided into 12 culture spaces, in culture space on two sidewalls recessing to engage culture tank, such as Fig. 1
It is shown, culture tank from top to bottom successively include the first culture tank 4, the second culture tank 5, as shown in Fig. 2, wherein the first culture tank 4,
By being cased with, the pin shaft 4.3 of torsional spring is hinged and articulated position is laid with rubber elastic membrane for second culture tank, 5 two sides cell wall and bottom wall
4.2,4.2 gluing of rubber membrane does not interfere the rotation of two cell walls, and both ends are made of elastic rubber wall, convenient for two cell walls to internal pressure,
And strainer 4.4 is set on bottom wall, closure door 4.5 is set below strainer 4.4, carries out repeating absorption and culture by closure door 4.5 and strainer 4.4
The replacement of liquid, the first 4 liang of culture tank cell wall top are provided with liquid-leaking nozzle 4.1, can first pick up top cover 2 when replacing culture solution, inject
Nutrient solution, with the increase of liquid measure, extra nutrient solution enters the second culture tank along liquid-leaking nozzle 4.1.
As a further improvement, as shown in figure 4,2 two sidewalls of the top cover are provided with eaves along 2.1, eaves along 2.1 outsides with
Card slot 1.3 is bonded, and inside is bonded culturing rack 3, by 2 sets of top cover on culturing rack 3, on the one hand makes the culture in culturing rack 3 empty
Between it is relatively independent, another aspect top cover 2, culturing rack 3 and pedestal 1 constitute closed culture space, reduce extraneous interference.
As a further improvement, 1 side of pedestal is provided with liquid outlet 1.2, and is connected to each culture sky by pipeline
Between, convenient for discharge waste liquid.
When use:
(1) cell to be separated is collected by centrifugation, is sufficiently suspended cell with a small amount of PBE Incubating Solution, is added corresponding primary antibody, 5 DEG C
It is incubated for 20 minutes, it is primary with appropriate PBE to wash cell after centrifugation, after centrifugation again plus after PBE sufficiently suspension cell, is transferred to culture tank
In, and the corresponding coated magnetic bead of secondary antibody is added, it mixes postposition and is incubated at room temperature 10~15 minutes.
(2) start the electromagnet 1.1 in top cover 2, it is static and magnetic-adsorption 5~10 minutes, close the electromagnet in top cover 2
1.1, top cover 2 is picked up, PBE is added, cell liquid is resuspended, the electromagnet 1.1 being then turned in top cover 2 recycles 2 times, opens in pedestal 1
Electromagnet 1.1 simultaneously close off the electromagnet 1.1 in top cover 2, after 1min, pick up top cover 2, open the envelope in the first culture tank 4
Door 4.5, the mixed liquid in the first culture tank 4 enter the second culture tank after the magnetic bead on strainer 4.4 and strainer 4.4, adsorb again,
Next subsequent directly culture or detection etc. can be carried out by the way that culture solution etc. is added.
This culture dish repeats adsorbing separation T cell, improves the separation quantity of T cell, and the culture tank of elastic hinge joint is convenient for
Assembling and replacement, expanded service life.
Certainly, the above is only the specific application examples of the utility model, do not constitute and appoint to the protection scope of the utility model
What is limited.Any technical scheme formed by adopting equivalent transformation or equivalent replacement, all falls within the utility model rights protection model
Within enclosing.
Claims (5)
1. a kind of culture apparatus for repeating to adsorb T cell, including pedestal, culturing rack and top cover, top cover is covered to be formed on culturing rack
Confined space isolation is extraneous, which is characterized in that the culturing rack includes the L-type end plate at both ends, between two end plates by laterally, it is longitudinal
Partition be separated into multiple culture spaces, recessing is on two sidewalls to engage culture tank in culture space, and culture tank is from top to bottom
It include successively the first culture tank, the second culture tank, wherein the first culture tank, the second culture tank two sides cell wall and bottom wall pass through set
There is the pin shaft of torsional spring hinged and articulated position laying rubber elastic membrane, both ends are made of elastic rubber wall, and set filter on bottom wall
Net, strainer lower section set closure door, and two cell wall top of the first culture tank is provided with liquid-leaking nozzle, is provided with electromagnet in top cover and pedestal.
2. a kind of culture apparatus for repeating to adsorb T cell according to claim 1, which is characterized in that the pedestal upper limb
If card slot, culturing rack is engaged.
3. a kind of culture apparatus for repeating to adsorb T cell according to claim 1 or 2, which is characterized in that the top cover two
Side wall is provided with eaves edge, and eaves is bonded along outside with card slot, and inside is bonded culturing rack.
4. a kind of culture apparatus for repeating to adsorb T cell according to claim 1, which is characterized in that at the top of the top cover
If electromagnet.
5. a kind of culture apparatus for repeating to adsorb T cell according to claim 1, which is characterized in that the pedestal side
It is provided with liquid outlet, and each culture space is connected to by pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820503348.6U CN208293022U (en) | 2018-04-10 | 2018-04-10 | A kind of culture apparatus for repeating to adsorb T cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820503348.6U CN208293022U (en) | 2018-04-10 | 2018-04-10 | A kind of culture apparatus for repeating to adsorb T cell |
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| Publication Number | Publication Date |
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| CN208293022U true CN208293022U (en) | 2018-12-28 |
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| CN201820503348.6U Active CN208293022U (en) | 2018-04-10 | 2018-04-10 | A kind of culture apparatus for repeating to adsorb T cell |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114377464A (en) * | 2021-12-23 | 2022-04-22 | 呼和浩特科林热电有限责任公司 | Desulfurization slurry filtering system of thermal power plant |
-
2018
- 2018-04-10 CN CN201820503348.6U patent/CN208293022U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114377464A (en) * | 2021-12-23 | 2022-04-22 | 呼和浩特科林热电有限责任公司 | Desulfurization slurry filtering system of thermal power plant |
| CN114377464B (en) * | 2021-12-23 | 2023-08-15 | 呼和浩特科林热电有限责任公司 | Desulfurization slurry filtering system of thermal power plant |
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