CN208172018U - Western blot instrument - Google Patents

Abstract

The utility model provides a kind of western blot instrument,By sample placement mechanism,Specimen discerning mechanism and incubation mechanism concentrated setting are in station,By sample suction component,Reagent output precision,Waste liquid cleaning assembly and image acquisition mechanism concentrated setting are on the robotic arm,So that complete machine structure is more compact,Since mechanical arm is only moving back and forth between the top side of first through hole and the top side of the second through-hole under the driving of the first driving assembly,So that the moving line of mechanical arm greatly simplifies,And it is substituted for since a monolith of traditional strip is incubated for disk and is vertically arranged and interchangeable two pieces of incubations disk,Two pieces are incubated for disk and can execute the operation of different function respectively,And then the time of detection is saved,So that detection process is more flexible,To solve western blot instrument because of the incubation disk using external strip,Lead to be incubated for mechanism vulnerable to external disturbance,Machine volume is big,Space utilization rate is poor,Moving line is complicated,The inflexible equal technical problem of detection process.

Description

Western blot instrument

Technical field

The utility model belongs to the technical field of Medical Equipment, is to be related to a kind of western blot instrument more specifically.

Background technique

Western blot instrument is characteristic, the equipment of expression and distribution situation for detecting immune protein, utilizes western blot Principle, by will be printed on the nitrocellulose filter solid phase carrier film item of antigen (be equivalent to be coated with) of protein band successively with After specific antibody and the effect of enzyme mark secondary antibody, the enzyme reaction substrate that can form insoluble colour developing object is added, dyes zone, Then it is taken pictures using camera to colour developing film item, picture upload software progress gray scale discriminance analysis is done into qualitative and semi-quantitative results Interpretation.Western blot instrument is mainly used for the various pathogen confirmations such as HIV, HCV, HTLV, HP and the correlations such as detection and rheumatism Autoantibody (ENA) detection, atopic allergen blotting detection and spectrotype blotting detection etc..

Currently, the incubation disk of external is usually used in western blot instrument in the market, it is incubated for the elongated layout of disk, The layout type will lead to following problems：One, the length of complete machine is too long, causes space utilization rate poor；Two, mechanism setting is incubated for exist The outside of equipment is vulnerable to external disturbance；Three, it is loaded the moving parts operation in three directions of this needs, causes moving line multiple It is miscellaneous, detection process is inflexible.

Utility model content

The purpose of this utility model is to provide a kind of western blot instrument, to solve in the prior art, western blot instrument because Using the incubation disk of external strip, cause incubation mechanism vulnerable to external disturbance, machine volume is big, space utilization rate is poor, fortune The technical problem that dynamic route is complicated, detection process is inflexible etc..

To achieve the goals above, the technical solution adopted in the utility model is：A kind of western blot instrument is provided, is wrapped It includes：

Station offers first through hole and the second through-hole on the table top of the station, is provided in the station Sample placement mechanism, the specimen discerning mechanism of bar code and for cultivating in the sample tube for identification for storing sample tube The incubation mechanism of sample, the sample placement mechanism include the sample basin that basin mouth is connected to the first through hole, the incubation machine Structure includes being movably connected on the bottom side of second through-hole and can alternately exposing to two incubation disks in second through-hole；

Mechanical arm is movably connected on the top side of the station, is provided on the mechanical arm for drawing and discharging institute State the sample suction component of sample, the reagent output precision for exporting reagent, the waste liquid cleaning assembly for drawing waste liquid and For shooting the image acquisition mechanism of film reactiveness；And

First driving assembly is connected on the table top of the station, for driving the mechanical arm logical in described first It is moved back and forth between the top side in hole and the top side of second through-hole.

Further, the mechanism that is incubated for further includes the shaking table for being used to support the incubation disk, and described for driving The third driving assembly that shaking table is swung.

Further, the incubation mechanism further includes being fixedly arranged on the heating component of the shaking table bottom, and for putting Set the dry component that the film item in the incubation disk is blowed.

Further, it is provided on the table top of the station and takes disk button with what the third driving assembly was electrically connected.

Further, the sample suction component includes for the sample to be transported to described incubate out of described sample tube The sampling probe in disk is educated, third through-hole is also provided on the table top of the station, the western blot instrument further includes being set to The 4th driving assembly for being used to drive the sampling needle lifting on the mechanical arm, and for cleaning the clear of the sampling probe Washing machine structure；The wiper mechanism includes being set in the station and service sink that Chi Kou is connected to the third through-hole, institute The one end for stating sampling probe can be inserted into the service sink.

Further, the sample placement mechanism further includes being placed in the sample basin to be used to fix the sample tube Rack, and the 5th driving assembly for driving rack rotation.

Further, inspection window is offered on the side wall of the sample basin, the specimen discerning mechanism includes for passing through The inspection window scans the scanner of bar code in the sample tube.

Further, the reagent output precision includes infusion mouth, and the western blot instrument further includes for by the examination Agent is pumped to the first pump in the infusion mouth.

Further, the waste liquid cleaning assembly includes the connecting rod group with the 4th driving assembly transmission connection, with And it is fixedly arranged on the imbibition nozzle of connecting rod group one end.

Further, described image collecting mechanism includes camera and the light source assembly for being fixedly arranged on the camera side.

The beneficial effect of western blot instrument provided by the utility model is：Know using by sample placement mechanism, sample Other mechanism and be incubated for mechanism concentrated setting in station, by sample suction component, reagent output precision, waste liquid cleaning assembly and Image acquisition mechanism concentrated setting on the robotic arm so that western blot instrument complete machine structure is more compact, since mechanical arm is The driving of one driving assembly lower moves back and forth between the top side of first through hole and the top side of the second through-hole, so that mechanical arm Moving line greatly simplifies, and make since a monolith of traditional strip to be incubated for disk and be substituted for be vertically arranged and can replace Two pieces of incubation disks reduce the occupancy length for being incubated for disk, and two pieces are incubated for disk and can execute the operation of different function respectively, And then the time of detection has been saved, so that detection process is more flexible, to efficiently solve western blot instrument because using outer The incubation disk for setting formula strip causes incubation mechanism vulnerable to external disturbance, machine volume is big, space utilization rate is poor, moving line The technical problem complicated, detection process is inflexible etc., reduces the volume of western blot instrument, improves the inspection of western blot instrument Survey efficiency.

Detailed description of the invention

It, below will be to embodiment or the prior art in order to illustrate more clearly of the technical scheme in the embodiment of the utility model Attached drawing needed in description is briefly described, it should be apparent that, the accompanying drawings in the following description is only that this is practical new Some embodiments of type for those of ordinary skill in the art without any creative labor, can be with Other attached drawings are obtained according to these attached drawings.

Fig. 1 is the stereoscopic schematic diagram of western blot instrument provided by the embodiment of the utility model；

Fig. 2 is the stereoscopic schematic diagram of another angle of western blot instrument provided by the embodiment of the utility model；

Fig. 3 is that western blot instrument provided by the embodiment of the utility model removes the stereoscopic schematic diagram after shell；

Fig. 4 is the three-dimensional signal that western blot instrument provided by the embodiment of the utility model removes another angle after shell Figure；

Fig. 5 is the stereoscopic schematic diagram provided by the embodiment of the utility model for being incubated for mechanism；

Fig. 6 is the stereoscopic schematic diagram of dry component provided by the embodiment of the utility model；

Fig. 7 is the stereoscopic schematic diagram of sample placement mechanism and specimen discerning mechanism provided by the embodiment of the utility model；

Fig. 8 is that mechanical arm provided by the embodiment of the utility model removes the stereoscopic schematic diagram after shell；

Fig. 9 is the stereoscopic schematic diagram that mechanical arm provided by the embodiment of the utility model removes another angle after shell.

Wherein, each appended drawing reference in figure：

It is 1-western blot instrument, 2-sample tubes, 10-stations, 20-mechanical arms, the 30-the first driving assembly, 40-whole Machine shell, 60-power supply modules, the 70-the first pump, 11-sample placement mechanisms, 12-specimen discerning mechanisms, 13-are incubated Educate mechanism, 15-take disk button, 21-sample suction components, 22-reagent output precisions, 23-waste liquid cleaning assemblies, 24-figure As collecting mechanism, the 25-the four driving assembly, the 41-the first avoid holes, the 42-the second avoid holes, 43-shields, 44-ventilations Hole, 51-master control borads, 52-interface boards, 53-protocol conversion plates, 101-first through hole, the 102-the second through-hole, 103-thirds Through-hole, 111-sample basins, 112-racks, the 113-the five driving assembly, 121-scanners, 122-mounting assemblies, 131-are incubated for disk, the 132-the second driving assembly, 133-shaking tables, 134-third driving assemblies, 135-dry components, 141- Service sink, 142-liquid pumps, the 143-the first solenoid valve, 211-sampling probes, 212-syringes, 213-first pressure sensors, 221-infusion mouths, 231-connection rod sets, 232-imbibition nozzles, 233-vacuum pumps, 234-second solenoid valves, 235-second pressures Sensor, 241-cameras, 242-light source assemblies, 1351-hair dryers, 1352-exhaust fans, 2311-drive rods, 2312-levers.

Specific embodiment

In order to which technical problem to be solved in the utility model, technical solution and beneficial effect is more clearly understood, with Lower combination accompanying drawings and embodiments, the present invention will be further described in detail.It should be appreciated that specific reality described herein It applies example to be only used to explain the utility model, is not used to limit the utility model.

It should be noted that it can be directly another when element is referred to as " being fixed on " or " being set to " another element On one element or indirectly on another element.When an element is known as " being connected to " another element, it can To be directly to another element or be indirectly connected on another element.

It is to be appreciated that term " length ", " width ", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientation or positional relationship of the instructions such as "horizontal", "top", "bottom" "inner", "outside" is that orientation based on the figure or position are closed System, is merely for convenience of describing the present invention and simplifying the description, rather than the device or element of indication or suggestion meaning are necessary It with specific orientation, is constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.

In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or Implicitly include one or more of the features.The meaning of " plurality " is two or two in the description of the present invention, More than, unless otherwise specifically defined.

Fig. 1 to Fig. 9 is please referred to, now western blot instrument provided by the utility model is illustrated.The western blot instrument 1 packet Include station 10, mechanical arm 20 and the first driving assembly 30, wherein offer first through hole on the table top of station 10 101 and second through-hole 102, it is provided with sample placement mechanism 11, specimen discerning mechanism 12 in station 10 and is incubated for mechanism 13, Herein, sample placement mechanism 11 is for storing sample tube 2, and specifically, sample placement mechanism 11 includes sample basin 111, sample basin 111 basin mouth is connected to first through hole 101；The bar code in sample tube 2 for identification of specimen discerning mechanism 12；Mechanism 13 is incubated for use In cultivating sample, specifically, being incubated for mechanism 13 includes two pieces of incubation disks 131, and two pieces of incubation disks 131 are movably connected in the second through-hole 102 bottom side, and two pieces of incubation disks 131 are alternately exposed in the second through-hole 102；Mechanical arm 20 is movably connected in operation The top side of platform 10, and be provided on mechanical arm 10 sample suction component 21, reagent output precision 22, waste liquid cleaning assembly 23 and Image acquisition mechanism 24, herein, sample suction component 21 is for absorption and emission sample, and reagent output precision 22 is for exporting examination Agent, waste liquid cleaning assembly 23 are used to shoot the reactiveness of film item for drawing waste liquid, image acquisition mechanism 24；First driving group Part 30 is connected on the table top of station 10, for driving top side and second through-hole 102 of the mechanical arm 20 in first through hole 101 It is moved back and forth between top side.

The working principle of western blot instrument 1：First the sample for needing to detect is put into sample tube 2, and in the outer of sample tube 2 Sample tube 2, is then put into sample basin 111 by the bar code that representative sample information is pasted on wall, then starts western blot instrument 1, The control mechanism (not marking) of western blot instrument 1 identifies sample tube according to preset computer program control specimen discerning mechanism 12 The information of sample in 2 then controls the top side that mechanical arm 20 is moved to first through hole 101, then controls sample suction component 21 Sample in sample tube 2 needed for drawing then controls the top side that mechanical arm 20 is moved to the second through-hole 102, then controls sample The sample of absorption is discharged into the incubation disk 131 for being placed with film item by suction component 21, then controls reagent output precision 22 to incubating It educates and injects reagent in disk 131, then control waste liquid cleaning assembly 23 and absorbed sample and reagent extra in disk 131 is incubated for, connect Waiting sample and reagent on film item after the reaction was completed, the figure that control mechanism control image acquisition mechanism 24 shows film item As being shot, and the image data collected is transferred to outer computer by wired or wireless mode and is stored It is analyzed with calculating, and then completes the detection of a sample, so above steps may be repeated multiple times, until the secondary Detection task is completed, Or until this time is exposed at the film item on the incubation disk 131 in the second through-hole 102 all using finishing, control mechanism can control hidden Another piece ensconced in station 10 is incubated for disk 131 and replaces the original position for being incubated for disk 131, continues to repeat the above steps to other Sample is detected, until all samples stored in sample placement mechanism 11 complete detection, is opened from starting western blot instrument 1 Begin, entire detection process can be completed in the case where unattended.

It can be understood that：First driving assembly 30 may include first motor and the first transmission component, wherein the first transmission Component connects first motor and mechanical arm 20, certainly, as the case may be and demand, in the other embodiments of the utility model In, the first driving assembly 30 may also include the first driving belt or sliding block etc., not limit uniquely herein；Control mechanism is settable It in the side of the first driving assembly 30, may also be arranged in station 10, for controlling sample placement mechanism 11, specimen discerning machine Structure 12, incubation mechanism 13, sample suction mechanism (not marking), reagent output mechanism (not marking), waste liquid cleaning mechanism (are not marked Note) and image acquisition mechanism 24 according to preset computer program run, specifically, control mechanism may include master control borad 51, connect Oralia 52 and protocol conversion plate 53 etc., wherein outside the first avoid holes 41 that interface board 52 passes through machine shell 40 are exposed to, and Rj45 interface, USB interface etc. are provided on interface board 52, protocol conversion plate 53 passes through the second avoid holes 42 of machine shell 40 Outside being exposed to, in this way, being manipulated convenient for technical staff to control mechanism.Certainly, as the case may be and demand, practical at this In novel other embodiments, control mechanism may also include other components, not limit uniquely herein.

Preferably, western blot instrument 1 further includes machine shell 40 and power supply module 60, is rotatablely connected on machine shell 40 There is shield 43, shield 43 can cover first through hole 101, the second through-hole 102 and mechanical arm 20, so that in pattern detection Dust in air-isolation and the foreign object that may surprisingly invade in the process, eliminate the risk of Interference Detection, ensure that sample is examined Survey the validity and accuracy of result；Power supply module 60 be arranged in aforesaid operations platform 10, and with sample placement mechanism 11, sample This identification mechanism 12 is incubated for mechanism 13, sample suction mechanism, reagent output mechanism, waste liquid cleaning mechanism, image acquisition mechanism 24 And control mechanism electrical connection, play the role of burning voltage and backup power supply, it is therefore prevented that external power supply powers off suddenly, avoids inspection The fortuitous events such as flow gauge interrupts, detection data is lost occur.

Western blot instrument 1 provided by the utility model, compared with prior art, beneficial effect is：Using by sample Placement mechanism 11, specimen discerning mechanism 12 and incubation 13 concentrated setting of mechanism are in station 10, by sample suction component 21, examination Agent output precision 22, waste liquid cleaning assembly 23 and 24 concentrated setting of image acquisition mechanism are on mechanical arm 20, so that western blot 1 complete machine structure of instrument is more compact, due to mechanical arm 20 under the driving of the first driving assembly 30 only in the top side of first through hole 101 And second through-hole 102 top side between move back and forth so that the moving line of mechanical arm 20 greatly simplifies, and due to that will pass One monolith of system strip, which is incubated for disk and is substituted for, to be vertically arranged and interchangeable two pieces of incubations disk 131, and incubation disk is reduced Occupancy length, and two pieces of incubation disks 131 can execute respectively the operation of different function, and then save the time of detection, make Detection process it is more flexible, thus efficiently solve western blot instrument because using external strip incubation disk, cause Be incubated for mechanism vulnerable to external disturbance, machine volume is big, space utilization rate is poor, moving line is complicated, detection process is inflexible etc. The technical issues of, the volume of western blot instrument 1 is reduced, the detection efficiency of western blot instrument 1 is improved.

Further, referring to Fig. 5, a kind of specific embodiment as western blot instrument provided by the utility model, Above-mentioned incubation mechanism 13 further includes shaking table 133 and third driving assembly 134, wherein shaking table 133 is used to support above-mentioned incubation disk 131, third driving assembly 134 is for driving shaking table 133 to swing.Specifically, being incubated for mechanism 13 further includes the second driving assembly 132, the second driving assembly 132 is used to that two pieces of incubation disks 131 to be driven alternately to be exposed in above-mentioned second through-hole 102, and the Two driving assemblies 132 include support base 1321 and the second motor 1322, and support base 1321 and the second motor 1322 are sequentially connected, the Two motors 1322 can drive support base 1321 to rotate around its central axes, and third driving assembly 134 is fastenedly connected in support base 1321 On, it can be rotated together with support base 1321, shaking table 133 and third driving assembly 134 are sequentially connected, and shaking table 133 includes the One shaking table fission and the second shaking table are seperated (not marking), and two pieces of incubation disks 131 are detachably connected to the first shaking table fission respectively On the second shaking table fission, in this way, starting the second motor 1322 when needing to replace incubation disk 131, the second motor 1322 is driven Support base 1321 rotates 180 ° and can be completed；Herein, the first shaking table fission and the second shaking table fission are distributed in support base 1321 The opposite sides of axis, and the first shaking table fission and the second shaking table fission can make one under the driving of third driving assembly 134 Tentering degree is swung up and down, and then is conducive to shake up in the sample being incubated in disk 131 and reagent.It can be understood that：Third is driven Dynamic component 134 may include third motor, third transmission shaft and be biased to wheel group, wherein third is driven axis connection third motor and partially It to wheel group, is biased to wheel group and shaking table 133 is sequentially connected, certainly, as the case may be and demand, in other realities of the utility model It applies in example, third driving assembly 134 may also include other components, not limit uniquely herein.

Further, referring to Fig. 6, a kind of specific embodiment as western blot instrument provided by the utility model, Above-mentioned incubation mechanism 13 further includes heating component (not marking) and dry component 135, wherein heating component is fixed at above-mentioned The bottom of shaking table 133, dry component 135 is for being blowed the film item being placed in above-mentioned incubation disk 131.Specifically, add Hot component includes heating film, and heating film is attached to the bottom for being incubated for disk 131, and being incubated for disk 131 is passed using with good heat The material for the property led is made；Dry component 135 include hair dryer 1351 and exhaust fan 1352, hair dryer 1351 towards be incubated for disk 131, And make air duct that the film item for being placed on and being incubated in disk 131 be completely covered, exhaust fan 1352 is by being provided with above-mentioned machine shell 40 On ventilation hole 44 by outside air be pumped into aforesaid operations platform 10 in, in this way, in heating film, hair dryer 1351 and exhaust fan 1352 Under collective effect, the drying efficiency of film item can be greatly improved.

Further, referring to Fig. 3, a kind of specific embodiment as western blot instrument provided by the utility model, It is provided on the table top of aforesaid operations platform 10 and takes disk button 15, disk button 15 is taken to be electrically connected with above-mentioned third driving assembly 134. When needing to take out or replace above-mentioned incubation disk 131, presses and take disk button 15, third driving assembly 134 can be started, pass through third Driving assembly 134 drives the incubation disk 131 being exposed in the second through-hole 102 to rise, and prominent outside the second through-hole 102, into And it is incubated for disk 131 convenient for taking out or replacing, improve the detection efficiency of western blot instrument 1.

Further, Fig. 1, Fig. 3, Fig. 4, Fig. 8 and Fig. 9 are please referred to, as western blot instrument provided by the utility model A kind of specific embodiment, above-mentioned sample suction component 21 include sampling probe 211, and sampling probe 211 is used for sample from above-mentioned sample It is transported in this pipe 2 in above-mentioned incubation disk 131, meanwhile, third through-hole 103 is also provided on the table top of aforesaid operations platform 10, Above-mentioned western blot instrument 1 further includes the 4th driving assembly 25 and wiper mechanism (not marking), wherein the setting of the 4th driving assembly 25 On above-mentioned mechanical arm 20, for driving sampling probe 211 to go up and down, the wiper mechanism is for cleaning sampling probe 211 comprising cleaning Pond 141, service sink 141 is arranged in station 10, and its Chi Kou is connected to third through-hole 103, for accommodating cleaning solution, is taken One end of sample needle 211 can be protruded into service sink 141.Specifically, the 4th driving assembly 25 is sequentially connected with sampling probe 211, and the 4th Driving assembly 25 can drive sampling probe 211 to make back and forth to go up and down along the vertical direction, it is to be appreciated that：4th driving assembly 25 can wrap Include the 4th motor and the 4th transmission component, wherein the 4th transmission component connects the 4th motor and sampling probe 211, certainly, according to tool Body situation and demand, in the other embodiments of the utility model, the 4th driving assembly 25 may also include other components, herein not Make unique limit.When sampling probe 211 needs to carry out suction sample, stock layout or cleaning, the 4th driving assembly 25 can drive sampling probe 211 It falls and protrudes into sample tube 2, be incubated in the reactive tank or service sink 141 of disk 131, when mechanical arm 20 needs mobile, 4 wheel driven Dynamic component 25 can drive sampling probe 211 to rise reset and move together with mechanical arm 20；Herein, western blot instrument 1 further includes note Emitter 212 and first pressure sensor 213, sampling probe 211, syringe 212 and first pressure sensor 213 form above-mentioned sample Suction mechanism, syringe 212 and first pressure sensor 213 can be fastenedly connected in the side or station 10 of mechanical arm 20, Wherein, syringe 212 is connected to sampling probe 211, for sample to be drawn and is distributed by sampling probe 211, first pressure Sensor 213 is used to detect the stopping state of sampling probe 211, it is to be understood that in order to complete the suction of sample, sample suction Mechanism further includes the components such as tracheae and the first pipe fitting, and one end of tracheae is connect by the first pipe fitting with sampling probe 211, tracheae The other end connect with syringe 212 by the first pipe fitting；Meanwhile wiper mechanism further includes liquid pump 142 and the first solenoid valve 143, liquid pump 142 may be provided in station 10, and the first solenoid valve 143 may be provided at 20 side of mechanical arm, take when needing to clean When sample needle 211, the 4th driving assembly 25 drives sampling probe 211 to be fallen and protrudes into service sink 141, then opens the first solenoid valve 143, start liquid pump 142, cleaning solution is pumped into sampling probe 211 to the inner wall of sampling probe 211 by the way of similar spring It is cleaned, while cleaning solution being pumped into service sink 141, the outer wall of sampling probe 211 is cleaned, and then by sampling probe 211 clean up, convenient for next sample is drawn and distributed, it is to be understood that in order to complete the clear of sampling probe 211 It washes, wiper mechanism further includes the components such as fluid reservoir, the first perfusion tube and the second pipe fitting, wherein the two of first perfusion tube End is separately connected fluid reservoir and liquid pump 142 by two the second pipe fittings, and the both ends of first perfusion tube pass through two second Pipe fitting is separately connected liquid pump 142 and the first solenoid valve 143, and the both ends of first perfusion tube pass through two the second pipe fittings point The first solenoid valve 143 and sampling probe 211 are not connected, and the both ends of first perfusion tube pass through two the second pipe fitting difference Liquid pump 142 and service sink 141 are connected, herein, the first solenoid valve 143 is preferably two three-way electromagnetic valves, for being connected to 211 He of sampling probe Liquid pump 142.In addition, the first float-type transmitter (not shown) is provided in service sink 141, for in service sink 141 The liquid level of cleaning solution is detected, and when the liquid level of cleaning solution is excessively high, triggers warning note, and starting waste liquid cleaning mechanism will Extra cleaning solution siphons away.

Further, referring to Fig. 7, a kind of specific embodiment as western blot instrument provided by the utility model, Above-mentioned sample placement mechanism 11 further includes rack 112 and the 5th driving assembly 113, wherein rack 112 is accommodated in above-mentioned sample In this basin 111, for fixing above-mentioned sample tube 2；5th driving assembly 113 is for driving rack 112 to rotate.Specifically, exist Multiple slots (not marking) is offered on rack 112, slot extends along the vertical direction, for inserting for sample tube 2；5th drives Dynamic component 113 includes the 5th motor and the 5th transmission shaft, and one end of the 5th transmission shaft is connect with the 5th motor drive, the 5th transmission The other end of axis passes through sample basin 111 and rack 112 is sequentially connected, and the 5th driving assembly 113 can drive the 5th transmission shaft to turn Dynamic, the 5th transmission shaft can drive rack 112 to rotate around the central axes of sample basin 111, certainly, as the case may be and demand, In the other embodiments of the utility model, the 5th driving assembly 113 further includes the 5th driving belt etc., is not limited uniquely herein It is fixed.

Further, 7 are please referred to, as a kind of specific embodiment of western blot instrument provided by the utility model, It is offered on the side wall of above-mentioned sample basin 111 inspection window (not marking), meanwhile, above-mentioned specimen discerning mechanism 12 includes scanner 121, scanner 121 is used to scan the bar code in above-mentioned sample tube 2 by inspection window.Specifically, the mistake rotated in rack 112 Cheng Zhong, sample tube 2 can only seriatim be exposed to the inside of inspection window, i.e., each through the light that inspection window scanner 121 issues In the bar code that a sample tube 2 can only be projected, the validity and accuracy of sample information identification ensure that；And specimen discerning Mechanism 12 further includes mounting assembly 122, and scanner 121 is fastenedly connected by mounting assembly 122 with sample basin 111, herein, installation Component 122 includes support plate, pinboard and mounting plate (not marking), and one end and the sample basin 111 of support plate are fastenedly connected, and is supported The other end of plate and one end of pinboard are fastenedly connected, and the relative position between support plate and pinboard is adjustable, pinboard The other end be fastenedly connected with mounting plate, and the relative position between pinboard and mounting plate is adjustable, and the fixation of scanner 122 is set It sets on a mounting board, in this way, by the adjustment of relative position between support plate, pinboard and mounting plate, adjustable scanner 121 Relative position between inspection window ensure that the validity and accuracy of sample information identification.

Further, Fig. 3, Fig. 4 and Fig. 9 are please referred to, one kind as western blot instrument provided by the utility model is specific Embodiment, mentioned reagent output precision 22 include infusion mouth 221, meanwhile, above-mentioned western blot instrument 1 further includes the first pumping group Part 70, the first pump 70 are used to be pumped to mentioned reagent in infusion mouth 221.Specifically, western blot instrument 1 further includes examination Agent storage tank (does not mark), and reagent output precision 22, the first pump 70 and reagent storage tank composition mentioned reagent export machine Structure, wherein the first pump 70 includes peristaltic pump, the second perfusion tube and third pipe fitting (not marking), when reagent storage tank When being arranged in aforesaid operations platform 10, peristaltic pump be may be provided in station 10, and the both ends of second perfusion tube pass through two Third pipe fitting is separately connected infusion mouth 221 and peristaltic pump, and the both ends of another the second perfusion tube pass through two third pipe fittings It is separately connected peristaltic pump and reagent storage tank, when adding reagent into the reactive tank of above-mentioned incubation disk 131, by peristaltic pump from examination Reagent is pumped in the reactive tank by the second perfusion tube and infusion mouth 221 in agent storage tank, and passes through the anti-of peristaltic pump The extension liquid on infusion mouth 221 is eliminated to movement, and then prevents from getting rid of the generation of liquid phenomenon.Certainly, as the case may be and demand, at this In the other embodiments of utility model, reagent storage tank may be provided at the outside of western blot instrument 1, pass through the second perfusion tube and egg White seal mark instrument 1 connects, and peristaltic pump may be provided at the side of mechanical arm 20, not limit uniquely herein.In addition, in the second perfusion tube On can connect liquid sensor (not shown), for detect the second perfusion tube in reagent storage, when reagent deficiency, triggering alarm And prompt the reagent storage tank for supplementing reagent to reagent storage tank or more renewing.

Further, Fig. 4 and Fig. 8 are please referred to, a kind of specific implementation as western blot instrument provided by the utility model Mode, above-mentioned waste liquid cleaning assembly 23 include connecting rod group 231 and imbibition nozzle 232, wherein connecting rod group 231 and the above-mentioned 4th Driving assembly 25 is sequentially connected, and imbibition nozzle 232 is fixed at one end of connecting rod group 231.Specifically, connecting rod group 231 is wrapped Drive rod 2311 and lever 2312 are included, one end of drive rod 2311 and the 4th driving assembly 25 are sequentially connected, drive rod 2311 One end of the other end and lever 2312 is hinged, and the other end and the imbibition nozzle 232 of lever 2312 are fastenedly connected, and lever 2312 with Above-mentioned mechanical arm 20 is rotatablely connected, and tie point is located at the one end at the 2311 place end of close drive rod of lever 2312, works as needs When sample extra in above-mentioned incubation disk 131 and reagent are removed, the certain stroke of above-mentioned 4th motor reversal simultaneously drives drive rod 2311 move up, and the one end that will drive lever 2312 during drive rod 2311 moves up moves up, Jin Er Under the action of lever principle, the one end of lever 2312 being fastenedly connected with imbibition nozzle 232 is moved down, and by imbibition nozzle 232 End is sent into the reactive tank for being incubated for disk 131.In this way, same set of 4th driving assembly 25 can not only drive sampling probe 211, but also can drive Dynamic imbibition nozzle 232, saves a set of driving assembly, reduces production cost, so that the structure of entire western blot instrument 1 is more stepped up It gathers.Herein, above-mentioned western blot instrument 1 further includes the second pump and waste collection tank (not marking), waste liquid cleaning assembly 23, Second pump and above-mentioned waste liquid cleaning mechanism is formed with waste collection tank, wherein the second pump includes vacuum pump 233, second solenoid valve 234, third perfusion tube and the 4th pipe fitting (not marking), when waste collection tank is arranged in aforesaid operations platform When in 10, vacuum pump 233 and second solenoid valve 234 are arranged in station 10, wherein the both ends of a third perfusion tube pass through Two the 4th pipe fittings are separately connected waste collection tank and vacuum pump 233, and the both ends of one article of third perfusion tube pass through two the 4th Pipe fitting is separately connected waste collection tank and second solenoid valve 234, and the both ends of one article of third perfusion tube are connect by two the 4th pipes Head is separately connected second solenoid valve 234 and imbibition nozzle 232, and the both ends of one article of third perfusion tube pass through two the 4th pipe fitting difference Second solenoid valve 234 and above-mentioned service sink 141 are connected, herein, second solenoid valve 234 is preferably three-way magnetic valve, for being connected to Waste collection tank, imbibition nozzle 232 and service sink 141, when needing to remove sample and reagent extra in the reactive tank, the second electricity Waste collection tank is connected to by magnet valve 234 with imbibition nozzle 232, starts vacuum pump 233, makes to generate negative pressure in waste collection tank, in turn Sample extra in reactive tank and reagent are sucked in waste collection tank by third perfusion tube and imbibition nozzle 232, work as service sink When first float-type transmitter is alarmed in 141, waste collection tank is connected to by second solenoid valve 234 with service sink 141, starts vacuum Pump 233 makes to generate negative pressure in waste collection tank, and then by third perfusion tube directly by cleaning solution excessive in service sink 141 It sucks in waste collection tank.Certainly, as the case may be and demand, in the other embodiments of the utility model, waste collection Tank may be provided at the outside of western blot instrument 1, is connect by third perfusion tube with western blot instrument 1, is not limited uniquely herein. In addition, waste liquid cleaning mechanism further includes second pressure sensor 235 and the second float-type transmitter (not shown), second pressure is passed Sensor 235 and the second float-type transmitter are arranged in waste collection tank, wherein second pressure sensor 235 is used for waste liquid Negative pressure in collecting tank is detected, and when negative pressure deficiency, triggers warning note, and start vacuum pump 233 to waste collection Tank is vacuumized；Second float-type transmitter is for detecting the liquid level of the waste liquid in waste collection tank, when waste liquid When liquid level is excessively high, triggering alarms and prompts to empty the waste collection tank of waste collection tank or replacement sky.

Further, referring to Fig. 9, a kind of specific embodiment as western blot instrument provided by the utility model, Above-mentioned image acquisition mechanism 24 includes camera 241 and light source assembly 242, wherein light source assembly 242 is fixed at camera 241 side.Specifically, camera 241 is towards the bottom end of above-mentioned mechanical arm 20, when mechanical arm 20 is located at above-mentioned second through-hole When 102 top side, camera 241, which can focus on, to be completed in reaction and the film item being oven-dried, and the state that the film item is shown Image recording is got off, at this point, light source assembly 242 plays the role of illumination, camera 241 passes through the image data of acquisition above-mentioned Control mechanism is transferred to outer computer and is stored and calculated analysis.

The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this Made any modifications, equivalent replacements, and improvements etc., should be included in the utility model within the spirit and principle of utility model Protection scope within.

Claims (10)

1. western blot instrument, which is characterized in that including：

Station offers first through hole and the second through-hole on the table top of the station, is provided with and is used in the station The sample placement mechanism of storing sample tube, the specimen discerning mechanism of bar code and for cultivating sample in the sample tube for identification Incubation mechanism, the sample placement mechanism includes the sample basin that basin mouth is connected to the first through hole, incubation mechanism packet It includes the bottom side for being movably connected on second through-hole and can alternately expose to two incubation disks in second through-hole；

Mechanical arm is movably connected on the top side of the station, is provided on the mechanical arm for drawing and discharging the sample It this sample suction component, the reagent output precision for exporting reagent, the waste liquid cleaning assembly for drawing waste liquid and is used for Shoot the image acquisition mechanism of film reactiveness；And

First driving assembly is connected on the table top of the station, for driving the mechanical arm in the first through hole It is moved back and forth between top side and the top side of second through-hole.

2. western blot instrument as described in claim 1, which is characterized in that the incubation mechanism further includes being used to support described incubate Educate the shaking table of disk, and the third driving assembly for driving the shaking table to swing.

3. western blot instrument as claimed in claim 2, which is characterized in that the incubation mechanism further includes being fixedly arranged on the shaking table The heating component of bottom, and the dry component for the film item being placed in the incubation disk to be blowed.

4. western blot instrument as claimed in claim 3, which is characterized in that be provided on the table top of the station and described The electrical connection of three driving assemblies takes disk button.

5. such as the described in any item western blot instrument of claim 2 to 4, which is characterized in that the sample suction component includes using It is also opened up on the sampling probe being transported to the sample out of described sample tube in the incubation disk, the table top of the station Have a third through-hole, the western blot instrument further include be set on the mechanical arm for driving the of the sampling needle lifting Four driving assemblies, and the wiper mechanism for cleaning the sampling probe；The wiper mechanism includes being set to the station One end of the service sink that interior and Chi Kou is connected to the third through-hole, the sampling probe can be inserted into the service sink.

6. western blot instrument as claimed in claim 5, which is characterized in that the sample placement mechanism further include be placed in it is described The rack for being used to fix the sample tube in sample basin, and the 5th driving group for driving the rack rotation Part.

7. western blot instrument as claimed in claim 6, which is characterized in that inspection window is offered on the side wall of the sample basin, The specimen discerning mechanism includes the scanner for scanning bar code in the sample tube by the inspection window.

8. western blot instrument as claimed in claim 5, which is characterized in that the reagent output precision includes infusion mouth, described Western blot instrument further includes the first pump for being pumped to the reagent in the infusion mouth.

9. western blot instrument as claimed in claim 5, which is characterized in that the waste liquid cleaning assembly includes and the 4 wheel driven The connecting rod group of dynamic component transmission connection, and it is fixedly arranged on the imbibition nozzle of connecting rod group one end.

10. western blot instrument as claimed in claim 5, which is characterized in that described image collecting mechanism includes camera and consolidates Set on the light source assembly of the camera side.