CN207908244U - Fully automatic blood cell isolates and purifies device - Google Patents
Fully automatic blood cell isolates and purifies device Download PDFInfo
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- CN207908244U CN207908244U CN201820373685.8U CN201820373685U CN207908244U CN 207908244 U CN207908244 U CN 207908244U CN 201820373685 U CN201820373685 U CN 201820373685U CN 207908244 U CN207908244 U CN 207908244U
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- mixing tube
- solenoid valve
- support base
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- 210000000601 blood cell Anatomy 0.000 title claims abstract description 10
- 210000004027 cell Anatomy 0.000 claims abstract description 73
- 210000004369 blood Anatomy 0.000 claims abstract description 43
- 239000008280 blood Substances 0.000 claims abstract description 43
- 238000000926 separation method Methods 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 20
- 238000011160 research Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000001914 calming effect Effects 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004801 process automation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
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Abstract
The utility model discloses fully automatic blood cells to isolate and purify device, relates generally to technical field of cell biology.Including support base, the top of the support base is equipped with supporting rack, the middle part at the top of the support base is equipped with mixing tube vertically, piston is equipped in the mixing tube, the middle part of the piston is equipped with rotary shaft, the top of the piston is equipped with rotating disk, the bottom of the piston is equipped with air pressure motor, the top of the mixing tube is equipped with support plate, the support plate is equipped with the separation agent entrance being connected to the upper space of mixing tube, whole blood inlet, aim cell liquid exports, the top of support frame as described above is equipped with cell and detaches microballon storage bag, blood taking bag, aim cell collecting bag, the top of the support base is equipped with air pump, the support base is equipped with controller.The beneficial effects of the utility model are：Buoyancy can be isolated and purified to cell processes automation, the problems such as capable of overcoming manual separation cell, time-consuming, cumbersome and result poor repeatability.
Description
Technical field
The utility model is related to technical field of cell biology, specifically fully automatic blood cell isolates and purifies device.
Background technology
Basic constitutional unit of the cell as human body and animal is the important base of scientific research institution and drug research mechanism
Plinth raw material.Almost all of Basic of Biology research and medicament research and development will use cell, and the product quality of cell is direct
It is related to the quality of entire scientific experiment and drug research.
Cell is frequently found in tissue or blood, accurately study certain specific cells just need by specific cells from
It isolates and purifies out in different types of tissue and blood, currently used cell isolation and purification method is manual separation side
Method, it is cumbersome there is time-consuming, as a result the shortcomings of poor repeatability.
Utility model content
Utility model aims to solve problems of the prior art, provide a kind of with whirlpool concussion mixing
Principle, buoyancy can be isolated and purified cell processes automation, capable of overcoming manual separation cell, time-consuming, it is cumbersome and
As a result fully automatic blood cell the problems such as poor repeatability isolates and purifies device.
The utility model to achieve the above object, is achieved through the following technical solutions：
Fully automatic blood cell isolates and purifies device, including support base, and the top of the support base is equipped with supporting rack, described
The middle part at the top of support base is equipped with mixing tube vertically, and piston is equipped in the mixing tube, and the middle part of the piston is equipped with rotation
Axis, the rotary shaft extend vertically through piston and are connect with piston rotation, and the top of the piston is equipped with rotating disk, the rotating disk
It is fixedly connected with rotary shaft, the bottom of the piston is equipped with air pressure motor, and the air pressure motor is connect with transmission of rotary axis, described
The top of mixing tube be equipped with support plate, the support plate be equipped be connected to the upper space of mixing tube separation agent entrance,
The top of whole blood inlet, the outlet of aim cell liquid, support frame as described above is thin equipped with cell separation microballon storage bag, blood taking bag, purpose
Born of the same parents' collecting bag, the cell separation microballon storage bag are connected to by the first pipeline with separation agent entrance, and the blood taking bag passes through
Second pipeline is connected to whole blood inlet, and the aim cell collecting bag passes through third pipeline and aim cell liquid outlet, institute
It states the first pipeline and is equipped with the first solenoid valve, second pipeline is equipped with second solenoid valve, the bottom of the outer wall of the mixing tube
Portion is equipped with vent valve, and the top of the support base is equipped with air pump, and the gas outlet of the air pump is equipped with three-way pipe, the threeway
One end of pipe is connected to by telescoping tube with air pressure motor, and the other end is connected to by the 4th pipeline with the lower space of mixing tube, institute
It states telescoping tube and is equipped with third solenoid valve, the 4th pipeline is equipped with the 4th solenoid valve, and the support base is equipped with controller,
First solenoid valve, second solenoid valve, third solenoid valve, the 4th solenoid valve, air pressure motor, air pump, vent valve with control
Device signal connection processed.
The bottom of the piston is equipped with guide rod, and the inner wall of the mixing tube is equipped with the guiding being adapted with guide rod
Set.
The prior art is compared, the beneficial effects of the utility model are：
1, for the cell separation microballon storage bag of the utility model for storing microballon separation agent, blood taking bag is complete for storing
Blood, aim cell collecting bag are used for the microballon liquid of the purposeful cell of collection belt, and mixing tube is divided into upper space under by piston
Portion space, when progress cell isolates and purifies, microballon separation agent, whole blood enter the upper space of mixing tube, air pump and air pressure
When motor is connected to, rotating disk can be driven to rotate, microballon separation agent can be made to be sufficiently mixed with whole blood, air pump and mixing tube
Lower space connection when, piston can be pushed up, the first solenoid valve, second solenoid valve, the can be controlled by controller
Buoyancy can be isolated and purified cell by the open and close of three solenoid valves, the 4th solenoid valve, air pressure motor, air pump, vent valve
The problems such as process automation, capable of overcoming manual separation cell, time-consuming, cumbersome and result poor repeatability.
2, the bottom of the piston of the utility model is equipped with guide rod, and the inner wall of the mixing tube is equipped with mutually fits with guide rod
The guide sleeve answered can prevent during air pressure motor starts, and piston deflects, and can ensure that the utility model uses
Stability.
Description of the drawings
Attached drawing 1 is the structural schematic diagram of the utility model.
Label shown in attached drawing：1, support base；2, supporting rack；3, mixing tube；4, piston；5, rotary shaft；6, rotating disk；7、
Air pressure motor；8, support plate；9, separation agent entrance, 10, whole blood inlet；11, aim cell liquid exports；12, cell separation is micro-
Pearl storage bag；13, blood taking bag；14, aim cell collecting bag；15, air pump；16, guide rod；17, guide sleeve；18, vent valve.
Specific implementation mode
With reference to specific embodiment, the utility model is expanded on further.It should be understood that these embodiments are merely to illustrate this
Utility model rather than limitation the scope of the utility model.In addition, it should also be understood that, in the content for having read the utility model instruction
Later, those skilled in the art can make various changes or modifications the utility model, and such equivalent forms equally fall within this Shen
It please limited range.
Described in the utility model is that fully automatic blood cell isolates and purifies device, including support base 1, the support base 1
Top is equipped with supporting rack 2, and the middle part at the top of the support base 1 is equipped with mixing tube 3, piston is equipped in the mixing tube 3 vertically
4, piston 4 can slide up and down in mixing tube 3, and mixing tube 3 is divided into upper space and lower space by piston 4, piston 4
Upside is upper space, and the downside of piston 4 is lower space.The middle part of the piston 4 is equipped with rotary shaft 5, and the rotary shaft 5 is hung down
It is directly rotatablely connected through piston 4 and with piston 4, the top of the piston 4 is equipped with rotating disk 6, the rotating disk 6 and rotary shaft 5
It is fixedly connected, rotating disk 6 can be rotated with rotary shaft 5, and the bottom of the piston 4 is equipped with air pressure motor 7, the air pressure motor
7 are sequentially connected with rotary shaft 5, and air pressure motor 7 can drive rotary shaft 5 to rotate.The top of the mixing tube 3 is equipped with support plate 8,
The support plate 8 is equipped with separation agent entrance 9, whole blood inlet 10, the aim cell liquid being connected to the upper space of mixing tube 3
The top of outlet 11, support frame as described above 2 is equipped with cell separation microballon storage bag 12, blood taking bag 13, aim cell collecting bag 14, carefully
Born of the same parents detach microballon storage bag 12 for storing microballon separation agent, and blood taking bag 13 is for storing whole blood, aim cell collecting bag 14
Microballon liquid for the purposeful cell of collection belt.The cell separation microballon storage bag 12 is entered by the first pipeline with separation agent
Mouth 9 is connected to, and the blood taking bag 13 is connected to by the second pipeline with whole blood inlet 10, and the aim cell collecting bag 14 passes through third
Pipeline and aim cell liquid outlet 11 be connected to, and first pipeline is equipped with the first solenoid valve, and second pipeline is equipped with the
The bottom of two solenoid valves, the outer wall of the mixing tube 3 is equipped with vent valve 18, and the top of the support base 1 is equipped with air pump 15, institute
The gas outlet for stating air pump 15 is equipped with three-way pipe, and one end of the three-way pipe is connected to by telescoping tube with air pressure motor 7, the other end
It is connected to the lower space of mixing tube 3 by the 4th pipeline, the telescoping tube is equipped with third solenoid valve, on the 4th pipeline
Equipped with the 4th solenoid valve, when air pump 15 is connected to air pressure motor 7, rotating disk 6 can be driven to rotate, microballon can be made to detach
Reagent is sufficiently mixed with whole blood, when air pump 15 is connected to the lower space of mixing tube 3, can push up piston 4, can be incited somebody to action
The microballon liquid with aim cell in the upper space of mixing tube 3 is back to aim cell collecting bag 14.On the support base 1
Equipped with controller, first solenoid valve, second solenoid valve, third solenoid valve, the 4th solenoid valve, air pressure motor 7, air pump
15, vent valve 18 is connect with controller signals, and when progress cell isolates and purifies, microballon separation agent, whole blood enter mixing tube 3
Upper space, the first solenoid valve, second solenoid valve, third solenoid valve, the 4th solenoid valve, air pressure can be controlled by controller
Buoyancy can be isolated and purified cell processes automation, can overcome by the open and close of motor 7, air pump 15, vent valve 18
The problems such as time-consuming for manual separation cell, cumbersome and result poor repeatability.
The micro- of purposeful antibody is connected when the utility model is used, detaching microballon storage bag 12 by cell and storing 30ml
Pearl separation agent stores the whole blood of 50ml by blood taking bag 13, opens the first solenoid valve by controller so that cell separation is micro-
Microballon separation agent in pearl storage bag 12 flows into the upper space of mixing tube 3, opens second solenoid valve by controller, makes
The whole blood obtained in blood taking bag 13 flows into the upper space of mixing tube 3, and air pump 15, third solenoid valve are opened by controller,
Rotating disk 6 starts to rotate, and separation agent and whole blood in mixing tube 3 is caused to be sufficiently mixed 10min, aim cell and carries at this time
The microballon of antibody is stood after fully combining to be connected with the microballon of aim cell and floats on 3 upper layer of mixing tube after ten minutes, remaining is thin
Born of the same parents sink to the bottom of the upper space of mixing tube 3 under gravity, close third solenoid valve by controller, open the 4th
The lower space of solenoid valve, mixing tube 3 is full of high pressure gas, after piston 10 is pushed up about 10cm, you can keeps band purposeful
The microballon liquid of cell is back in aim cell collecting bag 14, and controlling vent valve 18 by controller opens, can be by mixing tube 3
High pressure gas discharge in lower space so that piston 4 resets.
In order to ensure that stability that the utility model uses, the bottom of the piston 4 are equipped with guide rod 16, the mixing tube
3 inner wall is equipped with the guide sleeve 17 being adapted with guide rod 16, and guide rod 16 is matched with guide sleeve 17, can be prevented in gas
During pressure motor 7 starts, piston 4 deflects, and can ensure the stability that the utility model uses.
Embodiment：Described in the utility model is that fully automatic blood cell isolates and purifies device, including support base 1, the branch
The top for supportting seat 1 is equipped with supporting rack 2, and gate frame can be used in supporting rack 2, and the middle part at the top of the support base 1 is inlayed vertically
There are mixing tube 3, the bottom opening of mixing tube 3 to be supported the closing of seat 1, piston 4 is equipped in the mixing tube 3, piston 4 can be mixed
It closing and is slided up and down in pipe 3, mixing tube 3 is divided into upper space and lower space by piston 4, and the upside of piston 4 is upper space,
The downside of piston 4 is lower space.The bottom of the piston 4 is equipped with guide rod 16, and the inner wall of the mixing tube 3 is equipped with and leads
The guide sleeve 17 being adapted to bar 16, guide rod 16 are matched with guide sleeve 17, can prevent the process started in air pressure motor 7
Middle piston 4 deflects, and can ensure the stability that the utility model uses.The middle part of the piston 4 is equipped with rotary shaft 5, institute
It states rotary shaft 5 to extend vertically through piston 4 and be rotatablely connected with piston 4, the top of the piston 4 is equipped with rotating disk 6, the rotating disk
Use integral structure, rotating disk 6 that can be rotated with rotary shaft 5 between 6 and rotary shaft 5, the bottom of the piston 4 is equipped with gas
Pressure motor 7, the air pressure motor 7 are sequentially connected by connector sleeve and rotary shaft 5, and air pressure motor 7 can drive 5 turns of rotary shaft
It is dynamic.The top of the mixing tube 3 is equipped with support plate 8, and the support plate 8 is equipped with point being connected to the upper space of mixing tube 3
From reagent inlet 9, whole blood inlet 10, aim cell liquid outlet 11, the top of support frame as described above 2 is equipped with cell separation microballon storage
Bag 12, blood taking bag 13, aim cell collecting bag 14, cell separation microballon storage bag 12 are taken a blood sample for storing microballon separation agent
Bag 13 is used for the microballon liquid of the purposeful cell of collection belt for storing whole blood, aim cell collecting bag 14.It is set on support frame as described above 2
There are three hook, the cell separation microballon storage bag 12, blood taking bag 13, aim cell collecting bag 14 pass through hook and supporting rack
2 are detachably connected.The cell separation microballon storage bag 12 is connected to by the first pipeline with separation agent entrance 9, cell separation
Microballon storage bag 12 is located at the upside of separation agent entrance 9, and cell detaches the microballon separation agent in microballon storage bag 12 with certainly
Body gravity flows to separation agent entrance 9 along the first pipeline, and the blood taking bag 13 is connected to by the second pipeline with whole blood inlet 10,
Whole blood in blood taking bag 13 flows to whole blood inlet 10 with its own gravity along the second pipeline, and the aim cell collecting bag 14 passes through
Third pipeline is connected to aim cell liquid outlet 11.First pipeline is equipped with the first solenoid valve, and the first solenoid valve can be controlled
The break-make of the first pipeline is made, second pipeline is equipped with second solenoid valve, and second solenoid valve is used to control the logical of the second pipeline
It is disconnected.The bottom of the outer wall of the mixing tube 3 is equipped with vent valve 18, and vent valve 18 can be by the height in the lower space of mixing tube 3
Body of calming the anger quickly is discharged.The top of the support base 1 is equipped with air pump 15, and the gas outlet of the air pump 15 is equipped with three-way pipe,
One end of the three-way pipe is connected to by telescoping tube with air pressure motor 7, and the other end is empty by the lower part of the 4th pipeline and mixing tube 3
Between be connected to, the telescoping tube be equipped with third solenoid valve, the 4th pipeline be equipped with the 4th solenoid valve, when air pump 15 and gas
Pressure motor 7 be connected to when, can drive rotating disk 6 rotate, microballon separation agent can be made to be sufficiently mixed with whole blood, air pump 15 and
When the lower space connection of mixing tube 3, piston 4 can be pushed up, it can be purposeful by the band in the upper space of mixing tube 3
The microballon liquid of cell is back to aim cell collecting bag 14.The support base 1 is equipped with controller, and controller is based on microcontroller
Controller, first solenoid valve, second solenoid valve, third solenoid valve, the 4th solenoid valve, air pressure motor 7, air pump 15,
Vent valve 18 is connect with controller signals, and when progress cell isolates and purifies, microballon separation agent, whole blood enter mixing tube 3
Upper space can control the first solenoid valve, second solenoid valve, third solenoid valve, the 4th solenoid valve, air pressure horse by controller
Up to 7, air pump 15, the open and close of vent valve 18, buoyancy can be isolated and purified to cell processes automation, hand can be overcome
The problems such as time-consuming for dynamic separation cell, cumbersome and result poor repeatability.
Claims (2)
1. fully automatic blood cell isolates and purifies device, including support base (1), the top of the support base (1) is equipped with supporting rack
(2), it is characterised in that：The middle part at the top of the support base (1) is equipped with mixing tube (3) vertically, is equipped in the mixing tube (3)
The middle part of piston (4), the piston (4) is equipped with rotary shaft (5), and the rotary shaft (5) extends vertically through piston (4) and and piston
(4) it being rotatablely connected, the top of the piston (4) is equipped with rotating disk (6), and the rotating disk (6) is fixedly connected with rotary shaft (5),
The bottom of the piston (4) is equipped with air pressure motor (7), and the air pressure motor (7) is sequentially connected with rotary shaft (5), the mixing
The top for managing (3) is equipped with support plate (8), and the support plate (8) is equipped with the separation examination being connected to the upper space of mixing tube (3)
Agent entrance (9), whole blood inlet (10), aim cell liquid outlet (11), the top of support frame as described above (2) are equipped with cell and detach microballon
Storage bag (12), blood taking bag (13), aim cell collecting bag (14), the cell separation microballon storage bag (12) pass through the first pipe
Road is connected to separation agent entrance (9), and the blood taking bag (13) is connected to by the second pipeline with whole blood inlet (10), the purpose
Cell collecting bag (14) is connected to by third pipeline with aim cell liquid outlet (11), and first pipeline is equipped with the first electromagnetism
Valve, second pipeline are equipped with second solenoid valve, and the bottom of the outer wall of the mixing tube (3) is equipped with vent valve (18), described
The top of support base (1) is equipped with air pump (15), and the gas outlet of the air pump (15) is equipped with three-way pipe, and the one of the three-way pipe
End is connected to by telescoping tube with air pressure motor (7), and the other end is connected to by the 4th pipeline with the lower space of mixing tube (3), institute
It states telescoping tube and is equipped with third solenoid valve, the 4th pipeline is equipped with the 4th solenoid valve, and the support base (1) is equipped with control
Device, first solenoid valve, third solenoid valve, the 4th solenoid valve, air pressure motor (7), air pump (15), are put at second solenoid valve
Air valve (18) is connect with controller signals.
2. fully automatic blood cell according to claim 1 isolates and purifies device, it is characterised in that：The piston (4)
Bottom is equipped with guide rod (16), and the inner wall of the mixing tube (3) is equipped with the guide sleeve (17) being adapted with guide rod (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820373685.8U CN207908244U (en) | 2018-03-19 | 2018-03-19 | Fully automatic blood cell isolates and purifies device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820373685.8U CN207908244U (en) | 2018-03-19 | 2018-03-19 | Fully automatic blood cell isolates and purifies device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207908244U true CN207908244U (en) | 2018-09-25 |
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ID=63559190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820373685.8U Active CN207908244U (en) | 2018-03-19 | 2018-03-19 | Fully automatic blood cell isolates and purifies device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207908244U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112386756A (en) * | 2019-08-13 | 2021-02-23 | 萨斯特德特股份两合公司 | Separator and method for separating plasma and blood cells |
-
2018
- 2018-03-19 CN CN201820373685.8U patent/CN207908244U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112386756A (en) * | 2019-08-13 | 2021-02-23 | 萨斯特德特股份两合公司 | Separator and method for separating plasma and blood cells |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220615 Address after: 361012 unit 431, floor 4, building C, Xiamen international shipping center, No. 93, Xiangyu Road, Xiamen area, China (Fujian) pilot Free Trade Zone, Xiamen, Fujian Patentee after: Xiamen Sany hematopoietic Technology Co.,Ltd. Address before: 350108 building 10, innovation park, Haixi Park, high tech Zone, Fuzhou City, Fujian Province Patentee before: FUJIAN STEMERY TECHNOLOGY CO.,LTD. |
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| TR01 | Transfer of patent right |