CN207882283U - A kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system - Google Patents
A kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system Download PDFInfo
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- CN207882283U CN207882283U CN201721828120.6U CN201721828120U CN207882283U CN 207882283 U CN207882283 U CN 207882283U CN 201721828120 U CN201721828120 U CN 201721828120U CN 207882283 U CN207882283 U CN 207882283U
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- detecting system
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Abstract
The utility model belongs to fluoroimmunoassay technical field, it is related to a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system, including optical system, data transmission system and data processing system；Optical system includes image acquisition units, light source, narrow band filter slice, and the fluorescence immune chromatography strip mounting table immediately below it, fluorescence immune chromatography strip mounting table are equipped with the fluorescence immune chromatography strip card slot for placing fluorescence immune chromatography strip to be measured；The light source is located among image acquisition units, and narrow band filter is located at image acquisition units front end；The signal output end of optical system is connected by data transmission system with the signal input part of data processing system.The operation principle of whole system is handled the collected high-resolution fluorescence strip image of microscope, and the fluorescence signal of high information quantity is obtained, the final content for obtaining determinand in strip sample.The device has many advantages, such as that accuracy of detection is high, small, simple in structure and at low cost.
Description
Technical field
The utility model belongs to fluoroimmunoassay technical field, it be related to a kind of Portable fluorescence immunity-chromatography test strip at
As detecting system.
Background technology
Immunity-chromatography test strip detecting system is applied to biomedical detection, the every field such as food security and environment measuring.
With the development of technology, people are more and more stronger to the requirement of instant portable type accurately detected.Traditional immunochromatography scanning
Detecting system is complicated, and processing speed is slow.The principles of the present invention are that fluorescent marker is stimulated and will produce specific wavelength
Fluorescence.Fluorescence immune chromatography strip can be such that fluorescent marker and sample to be tested combines, and be sent out according to fluorescent marker glimmering
The power of light can quantify the concentration for detecting determinand.
Utility model content
To solve the problems, such as that immunochromatography scanning-detecting system traditional in background technology is complicated, processing speed is slow,
The utility model becomes the fluorescent collecting system that power microscope is combined using CMOS with hand-held.Not only solve conventional scanning systems
The shortcomings that, and improve the accuracy of system detectio.
The utility model is achieved through the following technical solutions：
A kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system, including optical system, data transmission system sum number
According to processing system；Optical system includes image acquisition units, light source, narrow band filter slice, and the fluorescence immunoassay immediately below it
Strip mounting table is chromatographed, fluorescence immune chromatography strip mounting table is equipped with the fluorescence for placing fluorescence immune chromatography strip to be measured
Immunity-chromatography test clamp bar slot, size adapt to the fluorescence area uniformity of the fluorescent image to ensure acquisition；The light source is located at figure
As among collecting unit, narrow band filter is located at image acquisition units front end；The signal output end of optical system is passed by data
Defeated system is connected with the signal input part of data processing system, collection optical system to clear strip image pass through data transmission
System inputs the data processing system in computer and calculates the content for obtaining determinand in fluorescence immunoassay test strips sample.
Preferably, described image collecting unit includes CMOS cameras and light microscope, and light microscope is set to CMOS
Before camera.CMOS cameras can be acquired the fluorescence signal of fluorescence immune chromatography strip, and utilize light microscope can
With the fluorescent image being more clear, and then obtain the higher handling result of precision.
Preferably, the resolution ratio of the CMOS cameras is 1280*1024.
Preferably, the enlargement ratio of the light microscope is 20~50x.
Preferably, the light microscope be hand-held become power microscope, using hand-held change power microscope have it is small, at
This low advantage.
Preferably, the light source is annular LED light source, generates the ultraviolet light of 4~400nm, and annular LED light sources make
Fluorescence strip is irradiated by uniform light.
Preferably, before the narrow band filter slice is placed in optical microphotograph lens head, bore is slightly less than light microscope top
Bore so that light microscope only receives fluorescence signal and eliminates the influence of stray light.
Preferably, wavelength 365nm centered on the parameter of the spike filter, half-wave peak width 30nm.
Preferably, when the light microscope and the operating distance of fluorescence immune chromatography strip are 9mm, light microscope
Enlargement ratio is set as 40x.
The beneficial effects of the utility model are：
The utility model is using the fluorescence signal of image-forming detecting system acquisition fluorescence immune chromatography strip, and once acquisition is all
Data, processing speed is fast, and it is that may be present not true during mechanical scanning to avoid general fluoroscopic examination scanning system
Determine factor.The device has many advantages, such as that accuracy of detection is high, small, simple in structure and at low cost.
Description of the drawings
Fig. 1 is a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system schematic front view of the utility model and annular
The top perspective view of LED light source and fluorescence immune chromatography strip mounting table.
Reference numeral：
1, CMOS camera；2, hand-held becomes power microscope；3, annular LED light source；4, spike filter；5, fluorescence immunoassay layer
Analyse strip card slot；6, fluorescence immune chromatography strip mounting table；7, fluorescence immune chromatography strip；8, data line；9, at data
Reason system.
Specific implementation mode
To be best understood from the utility model, the utility model is further described with reference to embodiment and attached drawing, with
Lower embodiment is only to illustrate to the utility model rather than be limited to it.
As shown in Figure 1, a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system is mainly passed by optical system, data
Defeated system and data processing system composition.Optical system includes CMOS cameras 1, hand-held change power microscope 2, annular LED light source
3, narrow band filter slice 4, fluorescence immune chromatography strip card slot 5, fluorescence immune chromatography strip mounting table 6, fluorescence immune chromatography strip
7.Hand-held becomes power microscope 2 and is set to before CMOS cameras 1, before narrow band filter slice is placed in hand-held change 2 camera lens of power microscope,
Its bore is slightly less than the bore that hand-held becomes 2 top of power microscope.
In detection process, by sample draw it is a certain amount of be added dropwise in the sample pad of fluorescence strip, sample passes through absorption pad
Siphonage enters bonding pad, and specific reaction is generated with fluorescent microsphere therein；Priority is detected survey line by the compound of generation
Antibody capture on (T lines) and control line (C lines).
Fluorescence immunoassay test strips after the completion of reaction process are inserted into fluorescence immune chromatography strip card slot 5.In annular LED
The fluorescent microsphere being trapped under the uniform irradiation of light source 3 on T lines and C lines can send out the fluorescence of specific wavelength, by narrow-band filtering
The filtration of piece 4 and hand-held become 2 amplification of power microscope and are acquired by CMOS cameras 1.And pass through data line 8
Data processing system 9 is transferred to be handled.Data processing system 9 is partitioned by image segmentation algorithm in fluorescence strip image
T lines and C lines region.It is excited the power to fluoresce, the concentration of rational judgment sample according to fluorescent microsphere on T lines and C lines.
The above content is the further descriptions done to the utility model in conjunction with specific preferred embodiment, can not
Assert that the specific implementation of the utility model is confined to these explanations.For the ordinary skill of the utility model technical field
For personnel, without departing from the concept of the premise utility, several improvement and replacement can also be made, should all belong to this
The protection domain of utility model.
Claims (9)
1. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system, it is characterised in that：Including optical system, data transmission
System and data processing system；Optical system includes image acquisition units, light source, narrow band filter slice, and immediately below it
Fluorescence immune chromatography strip mounting table, fluorescence immune chromatography strip mounting table are equipped with for placing fluorescence immune chromatography examination to be measured
The fluorescence immune chromatography strip card slot of item；The light source is located among image acquisition units, and narrow band filter is located at Image Acquisition
Unit front end；The signal output end of optical system is connected by data transmission system with the signal input part of data processing system.
2. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system according to claim 1, it is characterised in that：Institute
It includes CMOS cameras and light microscope to state image acquisition units, and light microscope is set to before CMOS cameras.
3. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system according to claim 2, it is characterised in that：Institute
The resolution ratio for stating CMOS cameras is 1280*1024.
4. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system according to claim 2, it is characterised in that：Institute
The enlargement ratio for stating light microscope is 20 ~ 50x.
5. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system according to claim 2 or 4, feature exist
In：The light microscope is that hand-held becomes power microscope.
6. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system according to claim 1, it is characterised in that：Institute
It is annular LED light source to state light source, generates the ultraviolet light of 4 ~ 400nm.
7. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system according to claim 2, it is characterised in that：Institute
It states before narrow band filter slice is placed in optical microphotograph lens head, bore is slightly less than the bore on light microscope top.
8. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system according to claim 1, it is characterised in that：Institute
State wavelength 365nm, half-wave peak width 30nm centered on the parameter of spike filter.
9. a kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system according to claim 2, it is characterised in that：Institute
When the operating distance for stating light microscope and fluorescence immune chromatography strip is 9mm, the enlargement ratio of light microscope is set as
40x。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721828120.6U CN207882283U (en) | 2017-12-22 | 2017-12-22 | A kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721828120.6U CN207882283U (en) | 2017-12-22 | 2017-12-22 | A kind of Portable fluorescence immunity-chromatography test strip image-forming detecting system |
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| CN207882283U true CN207882283U (en) | 2018-09-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111624331A (en) * | 2020-05-22 | 2020-09-04 | 军事科学院系统工程研究院卫勤保障技术研究所 | Multichannel synchronous fluorescence immunochromatography detection system |
-
2017
- 2017-12-22 CN CN201721828120.6U patent/CN207882283U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111624331A (en) * | 2020-05-22 | 2020-09-04 | 军事科学院系统工程研究院卫勤保障技术研究所 | Multichannel synchronous fluorescence immunochromatography detection system |
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Address after: 230088 biohan biological industrial park, northeast corner of the intersection of kongtai road and Chang'an Road, high tech Zone, Hefei, Anhui Province Patentee after: BIOHIT BIOTECHNOLOGY (HEFEI) Co.,Ltd. Address before: 230088 first to fourth floors, D9 building, Hefei Innovation Industrial Park, 800 Wangjiang West Road, high tech Zone, Hefei, Anhui Province Patentee before: BIOHIT BIOTECHNOLOGY (HEFEI) Co.,Ltd. |