CN207109001U - A kind of novel agent box - Google Patents
A kind of novel agent box Download PDFInfo
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- CN207109001U CN207109001U CN201721092583.0U CN201721092583U CN207109001U CN 207109001 U CN207109001 U CN 207109001U CN 201721092583 U CN201721092583 U CN 201721092583U CN 207109001 U CN207109001 U CN 207109001U
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Abstract
The utility model discloses a kind of novel agent box, it is related to field of biomedicine technology, the novel agent box includes lid, box body and cooling chamber, pore setter plate and spill mounting seat installed in tray interior, the bottom of box body is provided with cooling chamber, heat-conducting plate is provided between cooling chamber and tray interior, cooling chamber is internally provided with multiple independent cooling blocks, and the opening of cooling chamber is arranged on the side of box body, and sealing side door is provided with the opening of cooling chamber；The inwall both sides of box body are correspondingly provided with polylith spill mounting seat, and the both sides of pore setter plate are arranged in the mounting groove of spill mounting seat.The utility model is simple in construction, reasonable in design, by being arranged on multiple PCR pipes jacks and 96 hole PCR plates in pore setter plate, detection efficiency can be improved, it is short to shorten the cycle, there is Multiple detection, avoid pollution, independent cooling chamber and modularization cooling block is provided with the bottom of box body simultaneously, has ensured the quality of sample in pipe to greatest extent.
Description
Technical field
It the utility model is related to and be related to field of biomedicine technology, more particularly to a kind of novel agent box.
Background technology
PCR（PCR）Detection technique is that molecular biology often uses detection means, is generally used for detecting certain section
The presence of purpose nucleic acid sequence, and fluorescence quantitative PCR detection, because high sensitivity, detection cycle are short, automaticity height etc. is excellent
Gesture is widely used.Fluorescence quantitative PCR detection is generally carried out using quantitative real time PCR Instrument, and various reaction reagents are reacted in PCR
Mixed in pipe, then PCR reaction tubes are placed in quantitative real time PCR Instrument and start to react.The most cumbersome step in PCR detection process
Rapid is exactly to mix various samples in PCR reaction tubes because to add PCR buffer solutions, archaeal dna polymerase, primer, MgCl2,
DNTP and water etc., and these reagents are generally in dispersity, that is, these reagents bought all are to separate storage.In order to avoid
Reagent contamination, will change liquid transfer gun head when adding different reagents every time, the loss of these pipette tips also increases testing cost.It is this
Sample-adding flow consumes the time and adds testing cost.Thus be necessary to provide it is a kind of shorten testing process while reduce detect into
This detection kit.
Utility model content
In view of this, it is simple in construction, reasonable in design the utility model proposes a kind of novel agent box, by being arranged on
Multiple PCR pipes jacks and 96 hole PCR plates in pore setter plate, it is possible to increase detection efficiency, the shortening cycle is short, has multiple inspection
Survey, avoid the advantages that pollution, while independent cooling chamber and modularization cooling block is provided with the bottom of box body, protect to greatest extent
The quality of sample in pipe is hindered.
The utility model is solved the above problems by following technological means：
A kind of novel agent box, it is characterised in that including lid, box body and cooling chamber installed in tray interior, pipe
Hole setter plate and spill mounting seat, wherein：
Lid side is arranged on box body by hinge, and the opposite side of lid is fixed by snap on box body；
The bottom of box body is provided with cooling chamber, and heat-conducting plate, the inside of cooling chamber are provided between cooling chamber and tray interior
Multiple independent cooling blocks are provided with, the outer surface of cooling block is provided with longitudinal spacing groove, is provided with the inwall of cooling chamber
The longitudinal spacing boss adapted to limited impression, the opening of cooling chamber are arranged on the side of box body, pacify in the opening of cooling chamber
Equipped with sealing side door, seal the top of side door and be arranged on by hinge on box body, seal side door bottom it is fixed by snap
On box body；
The inwall both sides of box body are correspondingly provided with polylith spill mounting seat, and the both sides of pore setter plate are installed installed in spill
In the mounting groove of seat, multiple PCR pipes jacks and 96 hole PCR plates are offered in pore setter plate, use is consolidated with jack aperture
In the snap ring of banding PCR pipe；
The both sides of pore setter plate are provided with telescopic tongue, are provided with the top of telescopic tongue for controlling stretching for telescopic tongue position
Abbreviated dialing piece, one end of telescopic tongue coordinates with spill mounting seat to be fixed, and the other end of telescopic tongue is provided with base, and base is pacified with pore
Put and be provided with extension spring between plate two sides.
Further, the PCR pipes jack of the pore setter plate includes negative controls pore and positive control liquid pipe
Hole, negative controls pore and positive control solution pore are located at 96 hole PCR plates the same sides, pacify in the negative controls pore
Equipped with negative control liquid pipe, positive control liquid pipe is installed in the negative control liquid pipe.
Further, the structure of the 96 hole PCR plates is formed in one structure or demountable structure, the detachable knot
Structure is made up of support and 12 eight unions.
Further, in addition to reinforcing agent pipe is expanded, and amplification reinforcing agent pipe is provided with the pore setter plate
Hole, the amplification reinforcing agent pore are located at 96 hole PCR plate holes the same sides, and the amplification with the negative controls pore
Reinforcing agent pipe is separably in the amplification reinforcing agent pore.
Further, the 96 hole PCR plates include 96 PCR reaction tubes, and PCR is respectively arranged with the PCR reaction tubes
Reaction solution.
Further, negative controls are housed in the negative control liquid pipe, positive control is housed in positive control liquid pipe
Liquid.
Further, the negative control liquid pipe is different from the positive control liquid pipe specification.
Further, equipped with amplification reinforcing agent in the amplification reinforcing agent pipe.
A kind of novel agent box of the present utility model has the advantages that：
The utility model is simple in construction, reasonable in design：, can using detection of the kit of the present utility model to clinical sample
To complete PCR detections within 1.2 hours, detection cycle is short；Each PCR pipe in 96 hole PCR plates can accommodate different PCR reactions
Reagent, make multiple PCR detections reactions while carry out so that the flux of detection is higher；Kit includes positive control and feminine gender is right
According to avoiding the occurrence of due to reagent such as is contaminated at the reason and occurs false positive signal or gone out due to reasons such as reagent inactivations
Existing false negative signal；The PCR detection process of the detection kit uses fully closed detection, effectively PCR can be avoided to produce
The environmental pollution of thing.Simultaneously, it is ensured that PCR pipes are under cryogenic conditions all the time, it is ensured that the quality of sample in pipe, due to setting
Independent cooling block is equipped with, therefore it is more convenient to change cooling block.
Brief description of the drawings
In order to illustrate more clearly of the technical solution of the utility model, below by embodiment it is required use it is attached
Figure is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the present utility model, for
For those of ordinary skill in the art, on the premise of not paying creative work, other can also be obtained according to these accompanying drawings
Accompanying drawing.
Fig. 1 is a kind of external structure schematic diagram of novel agent box of the utility model；
Fig. 2 is a kind of internal structure schematic diagram of novel agent box of the utility model；
Fig. 3 is a kind of pore setter plate side structure schematic diagram of novel agent box of the utility model；
Fig. 4 is a kind of partial structural diagram of the pore setter plate of novel agent box of the utility model；
Fig. 5 is a kind of pore setter plate positive structure schematic of novel agent box of the utility model；
Fig. 6 is a kind of cooling block structural representation of novel agent box of the utility model.
The title of each number designation is respectively in figure：1- lids, 2- box bodys, 201- hinges, 202- sealings side door, 203- cards
Button, 2031- baffle plates, 2032- rotating shafts, 3- cooling chambers, 301- heat-conducting plates, 302- positive stop lug boss, 4- pores setter plate, 401- stretch
The flexible plectrum of tongue, 402-, 403- bases, 404- extension springs, 405-PCR pipes jack, 406- snap rings, 407-96 holes PCR plate, 5-
Spill mounting seat, 6- cooling blocks, 601- limited impressions.
Embodiment
In description of the present utility model, it is to be understood that term " " center ", " on ", " under ", "front", "rear",
The orientation or position relationship of the instruction such as "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " is based on accompanying drawing institutes
The orientation or position relationship shown, it is for only for ease of description the utility model and simplifies description, rather than indicates or imply and be signified
Device or element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to this practicality
New limitation.Term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity for indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can be expressed or
Implicitly include one or more this feature.In description of the present utility model, unless otherwise indicated, the implication of " multiple "
It is two or more.
The utility model is described in detail below with reference to accompanying drawing.
As shown in Figure 1, 2, a kind of novel agent box, including lid 1, box body 2 and the cooling chamber inside box body 2
3rd, pore setter plate 4 and spill mounting seat 5, wherein：The side of lid 1 by hinge 201 be arranged on box body 2 on, lid 1 it is another
Side is fixed on box body 2 by buckle 203；The bottom of box body 2 is provided with cooling chamber 3, is set between cooling chamber 3 and the inside of box body 2
Heat-conducting plate 301 is equipped with, cooling chamber 3 is internally provided with multiple independent cooling blocks 6, and the opening of cooling chamber 3 is arranged on box body 2
Side, sealing side door 202 is installed in the opening of cooling chamber 3, the top of sealing side door 202 is arranged on box by hinge 201
On body 2, the bottom of sealing side door 202 is fixed on box body 2 by buckle 203.
Specifically, buckle 203 includes baffle plate 2031 and rotating shaft 2032, baffle plate 2031 is arranged on box body 2 by rotating shaft 2032
On, lid 1 is realized by butterfly 2031 and seals the opening and closing of side door 202.
As shown in Figure 3,4, the inwall both sides of box body 2 are correspondingly provided with polylith spill mounting seat 5, and the two of pore setter plate 4
Side is arranged in the mounting groove of spill mounting seat 5, and the both sides of pore setter plate 4 are provided with telescopic tongue 401, the top of telescopic tongue 401
Portion is provided with the flexible plectrum 402 for controlling the position of telescopic tongue 401, and one end and the spill mounting seat 5 of telescopic tongue 401 coordinate admittedly
Fixed, the other end of telescopic tongue 401 is provided with base 403, and extension spring 404 is provided between base and the two sides of pore setter plate 4.It is more
Block spill mounting seat 5 can be met not with the cooperating of pore setter plate 4 with flexible modulation pore setter plate 4 and the height of bottom
The PCR pipe of same specification.
As shown in figure 5, multiple hole PCR plates 407 of PCR pipe jack 405 and 96 are offered in pore setter plate 4, in jack hole
Snap ring 406 for banding PCR pipe is consolidated with mouthful, by the circular hole of the flexible snap ring 406 of PCR pipe insert band, elasticity
Snap ring 406 can firmly fix PCR pipe, prevent PCR pipe from inadvertently falling off.
Specifically, the PCR pipe jack 405 of the pore setter plate 4 includes negative controls pore and positive control liquid pipe
Hole, negative controls pore and positive control solution pore are located at 96 hole PCR plates the same sides, pacify in the negative controls pore
Equipped with negative control liquid pipe, positive control liquid pipe is installed in the negative control liquid pipe.
Structure or demountable structure specifically, the structure of 96 hole PCR plate 407 is formed in one, the detachable knot
Structure is made up of support and 12 eight unions.Can take out in such cases the union of which part eight enter performing PCR detection and incite somebody to action
Other eight unions are preserved for later detection.In 96 orifice plate, for detecting one or more pathogen/microorganisms
PCR pipes can be represented with different colours or non-isolabeling, to avoid wrong sample-adding, improve sample-adding efficiency.
Specifically, also including amplification reinforcing agent pipe, and amplification reinforcing agent pipe is provided with the pore setter plate 4
Hole, the amplification reinforcing agent pore is located at the same side of 96 hole PCR plate 407 with the negative controls pore, and the amplification increases
Strong agent pipe is separably in the amplification reinforcing agent pore.
Specifically, 96 hole PCR plate 407 includes 96 PCR reaction tubes, it is anti-to be respectively arranged with PCR in the PCR reaction tubes
Answer liquid.
Specifically, being equipped with negative controls in the negative control liquid pipe, positive control solution is housed in positive control liquid pipe.
Specifically, the negative control liquid pipe is different from the positive control liquid pipe specification.
Specifically, equipped with amplification reinforcing agent in the amplification reinforcing agent pipe.
It should be noted that negative controls pore is provided in round, and coordinate with negative control liquid pipe, positive control solution
Pore is also configured as circle and coordinated with positive control liquid pipe.Negative control liquid pipe and positive control liquid pipe in a particular embodiment
Can be the reagent bottle of same size or the reagent bottle of different size, negative control liquid pipe and positive control liquid pipe can
Think, but be not limited to, one or both of 0.5ml, 1.5ml, 2ml and 5ml screw socket pipe/centrifuge tube.In preferred embodiment
Negative control liquid pipe and positive control liquid pipe are of different sizes to identify and can prevent to be loaded the maloperations such as mistake.
As shown in fig. 6, the outer surface of cooling block 6 is provided with longitudinal spacing groove 601, it is provided with the inwall of cooling chamber 3
The longitudinal spacing boss 302 adapted to limited impression 601.On the one hand the setting of positive stop lug boss 302, limited impression 601 is easy to cold
But block 6 is installed, and is placed in cooling chamber 3 and is rocked, and on the other hand can be worked independently using 1,2 or 3 cooling block 6.
A kind of novel agent box of the present utility model has the advantages that：
The utility model is simple in construction, reasonable in design：, can using detection of the kit of the present utility model to clinical sample
To complete PCR detections within 1.2 hours, detection cycle is short；Each PCR pipe in 96 hole PCR plates 407 can accommodate different PCR
Reaction reagent, make multiple PCR detections reactions while carry out so that the flux of detection is higher；Kit includes positive control and the moon
Property control, avoid the occurrence of due to reagent such as is contaminated at the reason and occurs false positive signal or due to reasons such as reagent inactivations
And the false negative signal occurred；The PCR detection process of the detection kit uses fully closed detection, can effectively avoid
The environmental pollution of PCR primer.Simultaneously, it is ensured that PCR pipes are under cryogenic conditions all the time, it is ensured that the quality of sample in pipe, by
In being provided with independent cooling block 6, therefore it is more convenient to change cooling block 6.
The multipair PCR primer for being used to detect different pathogens can be included in the present embodiment, in each PCR reaction tubes, i.e.
Continue multi-PRC reaction in each PCR reaction tubes, while difference can also be carried out in the different PCR reaction tubes of 6 hole PCR plates
PCR reacts so that the flux of detection is higher.Such as when being detected for respiratory pathogen, the multiple PCR detection kit can
Detect 10-12 sample, each pattern detection 15-23 kind respiratory pathogens simultaneously.
Finally it should be noted that：Above example is only to illustrate the technical solution of the utility model, rather than its limitations；
Although the utility model is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：
It can still modify to the technical scheme described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the utility model technology
The spirit and scope of scheme.
Claims (8)
1. a kind of novel agent box, it is characterised in that including lid, box body and cooling chamber installed in tray interior, pore
Setter plate and spill mounting seat, wherein：
Lid side is arranged on box body by hinge, and the opposite side of lid is fixed by snap on box body；
The bottom of box body is provided with cooling chamber, and heat-conducting plate is provided between cooling chamber and tray interior, and the inside of cooling chamber is set
There are multiple independent cooling blocks, the outer surface of cooling block is provided with longitudinal spacing groove, is provided with and limits on the inwall of cooling chamber
The longitudinal spacing boss that position groove adapts to, the opening of cooling chamber are arranged on the side of box body, are provided with the opening of cooling chamber
Side door is sealed, the top for sealing side door is arranged on box body by hinge, and the bottom for sealing side door is fixed by snap in box body
On；
The inwall both sides of box body are correspondingly provided with polylith spill mounting seat, and the both sides of pore setter plate are arranged on spill mounting seat
In mounting groove, multiple PCR pipes jacks and 96 hole PCR plates are offered in pore setter plate, are consolidated with jack aperture for binding round
The snap ring of tight PCR pipe；
The both sides of pore setter plate are provided with telescopic tongue, are provided with the top of telescopic tongue for controlling the flexible of telescopic tongue position to dial
Piece, one end of telescopic tongue coordinates with spill mounting seat to be fixed, and the other end of telescopic tongue is provided with base, base and pore setter plate
Extension spring is provided between two sides.
2. novel agent box as claimed in claim 1, it is characterised in that the PCR pipe jacks of the pore setter plate include
It is same that negative controls pore and positive control solution pore, negative controls pore and positive control solution pore are located at 96 hole PCR plates
Side, negative control liquid pipe is installed in the negative controls pore, positive control is installed in the negative control liquid pipe
Liquid pipe.
3. novel agent box as claimed in claim 2, it is characterised in that the structure of the 96 hole PCR plates is formed in one
Structure or demountable structure, the demountable structure are made up of support and 12 eight unions.
4. novel agent box as claimed in claim 3, it is characterised in that also include amplification reinforcing agent pipe and disposed in pore
Amplification reinforcing agent pore is provided with plate, it is same that amplification reinforcing agent pore with negative controls pore is located at 96 hole PCR plate holes
Side and amplification reinforcing agent pipe are separably located in the amplification reinforcing agent pore.
5. novel agent box as claimed in claim 4, it is characterised in that the 96 hole PCR plates include 96 PCR and reacted
Manage, PCR reaction solutions are respectively arranged with the PCR reaction tubes.
6. novel agent box as claimed in claim 5, it is characterised in that negative control is housed in the negative control liquid pipe
Liquid, positive control solution is housed in positive control liquid pipe.
7. novel agent box as claimed in claim 6, it is characterised in that the negative control liquid pipe and the positive control solution
Pipe specification is different.
8. novel agent box as claimed in claim 7, it is characterised in that equipped with amplification enhancing in the amplification reinforcing agent pipe
Agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721092583.0U CN207109001U (en) | 2017-08-29 | 2017-08-29 | A kind of novel agent box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721092583.0U CN207109001U (en) | 2017-08-29 | 2017-08-29 | A kind of novel agent box |
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| Publication Number | Publication Date |
|---|---|
| CN207109001U true CN207109001U (en) | 2018-03-16 |
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ID=61586502
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721092583.0U Active CN207109001U (en) | 2017-08-29 | 2017-08-29 | A kind of novel agent box |
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| CN (1) | CN207109001U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439737A (en) * | 2019-01-09 | 2019-03-08 | 赵志军 | A kind of kit and its diagnostic device for cerebrospinal fluid difficulty pathogen diagnosis |
-
2017
- 2017-08-29 CN CN201721092583.0U patent/CN207109001U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439737A (en) * | 2019-01-09 | 2019-03-08 | 赵志军 | A kind of kit and its diagnostic device for cerebrospinal fluid difficulty pathogen diagnosis |
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