CN211263284U - Magnetic device for preparing working electrode of electrochemical sensor - Google Patents
Magnetic device for preparing working electrode of electrochemical sensor Download PDFInfo
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- CN211263284U CN211263284U CN201920884512.7U CN201920884512U CN211263284U CN 211263284 U CN211263284 U CN 211263284U CN 201920884512 U CN201920884512 U CN 201920884512U CN 211263284 U CN211263284 U CN 211263284U
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Abstract
The utility model provides a can be used to prepare electrochemical sensor working electrode's magnetic means belongs to electrochemical sensor preparation technical field. It has solved the device scheduling problem that is applicable to this experimental study at present, a can be used to prepare electrochemical sensor working electrode's magnetic means, including the base, power supply scraps of paper one end has been seted up to base one side and has been inserted the insertion groove, and the base is provided with the recess, and the base still is provided with inlet channel and the liquid outlet channel with the recess intercommunication, and inlet channel is used for the entering of eluant, and liquid outlet channel is used for the outflow of eluant, and the bottom of base is provided with the bottom plate, is provided with the magnetic path on the bottom plate, and the magnetic path is located under electrode scraps of paper carbon electrode, the utility model has the advantages of effectual drop that prevents the.
Description
Technical Field
The utility model belongs to the technical field of electrochemical sensor preparation facilities, in particular to can be used to prepare electrochemical sensor working electrode's magnetic means.
Background
Ovarian cancer is a common malignant tumor of the female reproductive system, and the mortality rate is the first of gynecological malignant tumors. The early accurate and sensitive detection of the ovarian cancer is the key for improving the prognosis of ovarian cancer patients, and has very important significance for guaranteeing the health of women.
The combined detection of specific gene methylation is a biomarker for diagnosing ovarian cancer, and can improve the sensitivity and specificity of early diagnosis. For the convenience of detection, an electrochemical sensor, namely an electrode paper is commonly used, the electrode paper adopts a screen printing process, and a reference electrode and a counter electrode are miniature silver/silver chloride electrodes. And aiming at a special biomarker, the working electrode needs to be formed by modifying various compounds on the carbon electrode.
During the experimental research, a magnetic bead layer coated by avidin, a biotinylated DAN single-chain layer, a DNA complementary single-chain layer, a DNA methylation transferase layer, a MeCP2 protein layer with a GST label and an enzyme-labeled antibody layer resisting the GST label are required to be modified on a carbon electrode.
In order to prevent the decorative material from falling off, the decorative material needs to be adsorbed by a magnetic material. At present, no device suitable for the experimental study exists.
Disclosure of Invention
The utility model aims at the above-mentioned problem that exists among the prior art, provide a magnetic means that can be used to preparation electrochemical sensor working electrode.
The purpose of the utility model can be realized by the following technical proposal: the utility model provides a can be used to prepare electrochemical sensor working electrode's magnetic means, a serial communication port, which comprises a base, power supply scraps of paper one end is seted up to base one side and is inserted the insertion groove, the base be provided with the recess, the base still be provided with the inlet channel and the liquid outlet channel of recess intercommunication, inlet channel be used for the entering of eluant, liquid outlet channel be used for the outflow of eluant, the bottom of base is provided with the bottom plate, the bottom plate on be provided with the magnetic path, the magnetic path be located electrode scraps of paper carbon electrode under.
The utility model discloses a theory of operation: the utility model discloses the during operation, insert the one end of electrode scraps of paper to the recess in from the insertion groove, insert the back, the one end of electrode scraps of paper is located the recess, the bottom plate sets up in the bottom of base, magnetic path on the bottom plate is located electrode scraps of paper carbon electrode under, the magnetic bead of avidin peridium is added at first in toward the recess through the opening of recess, under the adsorption of magnetic path, the magnetic bead of avidin peridium enriches on electrode scraps of paper carbon electrode, then loop through the opening of recess and add required compound in toward the recess, in this process, because be the combination of specificity between the compound, in order to prevent that compound nonspecific from adsorbing on the one end of electrode scraps of paper or the lateral wall of recess, it elutes to need the eluant, for example, take after the addition of the MeCP2 protein of GST label, react for a period in the recess, add behind the elution nonspecific combination of eluant take the MeCP2 protein of, and then adding an enzyme-labeled antibody resisting the GST label, so that in the process of modifying and forming the working electrode, elution of eluent is required, and the eluent flows in from a liquid inlet pipeline and flows out from a liquid outlet pipeline. And after the preparation is finished, washing the surfaces of the groove and the working electrode by using eluent to obtain a modified electrode paper sheet. There are many types of eluents, and the kinds of eluents differ for eluting different compounds.
In the above magnetic device for preparing the working electrode of the electrochemical sensor, the magnetic device further comprises a detection table, the detection table is provided with a port, the port is used for inserting the end of the electrode paper sheet outside the insertion groove, and the detection table is used for detecting the electrochemical information of the electrode paper sheet.
In the above magnetic device for preparing the working electrode of the electrochemical sensor, the port is provided with a connecting end, the connecting end is respectively in contact connection with the working electrode, the reference electrode and the counter electrode, and the connecting end is used for being externally connected with a computer.
In the magnetic device for preparing the working electrode of the electrochemical sensor, the detection table is provided with a clamping groove, the base is provided with a clamping block, and when one end of the electrode paper sheet positioned outside the insertion groove is inserted into the port, the clamping block is inserted into the clamping groove.
In the magnetic device for preparing the working electrode of the electrochemical sensor, the peripheral wall of the insertion groove is provided with the sealing ring.
In the magnetic device for preparing the working electrode of the electrochemical sensor, the base plate is detachably connected with the base.
In the above magnetic device for preparing the working electrode of the electrochemical sensor, the bottom of the base is provided with a slot into which the bottom plate is inserted, the base is provided with a fixing plate for fixing the base, the two ends of the base are provided with insertion holes, and the fixing plate is inserted into the insertion holes to fix the bottom plate.
In foretell a magnetic means for preparing electrochemical sensor working electrode, inlet channel and outlet channel all be provided with the check valve, outlet channel still be connected with the drain pipe, the drain pipe be provided with the stagnant water and press from both sides, inlet channel be connected with the inlet channel.
Compared with the prior art, the utility model has the advantages of it is following:
1. the utility model discloses the during operation, insert the one end of electrode scraps of paper to the recess in from the insertion groove, insert the back, the one end of electrode scraps of paper is located the recess, the bottom plate sets up in the bottom of base, magnetic path on the bottom plate is located electrode scraps of paper carbon electrode under, the magnetic bead of avidin peridium is added at first in toward the recess through the opening of recess, under the adsorption of magnetic path, the magnetic bead of avidin peridium enriches on electrode scraps of paper carbon electrode, then loop through the opening of recess and add required compound in toward the recess, in this process, because be the combination of specificity between the compound, in order to prevent that compound nonspecific from adsorbing on the one end of electrode scraps of paper or the lateral wall of recess, it elutes to need the eluant, for example, take after the addition of the MeCP2 protein of GST label, react for a period in the recess, add behind the elution nonspecific combination of eluant take the MeCP2 protein of, and then adding an enzyme-labeled antibody resisting the GST label, so that in the process of modifying and forming the working electrode, elution of eluent is required, and the eluent flows in from a liquid inlet pipeline and flows out from a liquid outlet pipeline. And after the preparation is finished, washing the surfaces of the groove and the working electrode by using eluent to obtain a modified electrode paper sheet. There are many types of eluents, and the kinds of eluents differ for eluting different compounds.
2. The utility model discloses a need pass through the magnetic path and adsorb the modification material, effectually prevent to modify droing of material. And the groove is used as a reaction pool, so that various reactions can be carried out in the groove when the carbon electrode is modified.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
FIG. 2 is a schematic structural view of a fixing sheet of the present invention;
FIG. 3 is a schematic structural diagram of the bottom plate of the present invention;
fig. 4 is a schematic structural diagram of the liquid inlet channel and the liquid outlet channel of the present invention;
FIG. 5 is a schematic structural view of the inspection table of the present invention;
fig. 6 is a schematic structural diagram of the electrode paper sheet of the present invention.
In the figure, 1, a base; 2. inserting the groove; 3. a groove; 4. a liquid inlet channel; 5. a liquid outlet channel; 6. a base plate; 7. a magnetic block; 8. a detection table; 9. a port; 10. a connecting end; 11. a clamping groove is formed; 12. clamping the block; 13. A seal ring; 14. grooving; 15. a fixing sheet; 16. an insertion hole; 17. a one-way valve; 18. a liquid outlet pipe; 19. A water stop clip; 20. a liquid inlet pipe; 21. a working electrode; 22. a counter electrode; 23. a reference electrode.
Detailed Description
The following are specific embodiments of the present invention and the accompanying drawings are used to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.
As shown in fig. 1-6, a magnetic device for preparing a working electrode of an electrochemical sensor comprises a base 1, wherein one side of the base 1 is provided with a power supply paper sheet, one end of the power supply paper sheet is inserted into an insertion groove 2, the base 1 is provided with a groove 3, the base 1 is further provided with a liquid inlet channel 4 and a liquid outlet channel 5 which are communicated with the groove 3, the liquid inlet channel 4 is used for entering of eluent, the liquid outlet channel 5 is used for flowing out of the eluent, the bottom of the base 1 is provided with a bottom plate 6, the bottom plate 6 is provided with a magnetic block 7, and the magnetic block 7 is positioned right.
In further detail, the electrochemical detection device further comprises a detection table 8, the detection table 8 is provided with a port 9, the port 9 is used for inserting the end, located outside the insertion groove 2, of the electrode paper sheet, and the detection table 8 is used for detecting the electrochemical information of the electrode paper sheet. The port 9 is provided with a connecting end 10, the connecting end 10 is respectively in contact connection with the working electrode 21, the reference electrode 23 and the counter electrode 22, and the connecting end 10 is used for being externally connected with a computer. The test table 8 is provided with a card-in slot 11, the base 1 is provided with a card-in block 12, and when the end of the electrode paper sheet outside the insertion slot 2 is inserted into the port 9, the card-in block 12 is inserted into the card-in slot 11. After the working electrode 21 of the electrode paper is modified, a substance which can perform enzymatic reaction with enzyme in an enzyme-labeled antibody resisting a GST label is added, then the other end (positioned outside the insertion groove 2) of the electrode paper is inserted into the port 9, the clamping block 12 of the base 1 is inserted into the clamping groove 11, the base 1 is connected with the detection table 8, the connecting ends 10 are respectively in contact connection with the working electrode 21, the reference electrode 23 and the counter electrode 22, the number of the connecting ends 10 is 3, then the connecting ends 10 are connected with a computer, electrochemical information is displayed on a computer display screen after being processed by the computer, and an experimenter can reversely deduce the level of methylation of a specific gene according to the added substance of the enzymatic reaction.
More specifically, the peripheral wall of the insertion groove 2 is provided with a gasket 13. The sealing ring 13 is to prevent liquid from leaking from the insertion groove 2 in the process of forming a carbon electrode by modifying after the insertion of the electrode paper piece.
In further detail, the base plate 6 is detachably connected to the base 1. The base 1 is provided at the bottom thereof with a slot 14 into which the base plate 6 is inserted, the base 1 is provided with fixing pieces 15 for fixing the base 1, both ends of the base 1 are provided with insertion holes 16, and the fixing pieces 15 are inserted into the insertion holes 16 to fix the base plate 6. The bottom plate 6 is inserted into the base 1 from the slot 14, the fixing piece 15 is inserted into the fixing bottom plate 6 from the insertion hole 16, and the magnetic block 7 is positioned right below the carbon electrode of the electrode paper sheet after the bottom plate 6 is inserted.
In further detail, the liquid inlet pipe 20 and the liquid outlet pipe 18 are both provided with a one-way valve 17, the liquid outlet pipe 18 is also connected with the liquid outlet pipe 18, the liquid outlet pipe 18 is provided with a water stop clamp 19, and the liquid inlet pipe 20 is connected with the liquid inlet pipe 20. And (3) enabling the eluent to flow out of the liquid inlet pipe 20, opening the water stop clamp 19, enabling the eluent to flow in the groove 3, continuously eluting the electrode paper sheet by the eluent, and introducing air to blow away the liquid in the groove 3 after the electrode paper sheet is cleaned. When the experimenter adds the reaction liquid into the groove 3 to modify the electrode, the water stop clamp 19 is closed. The check valve 17 makes the liquid flow in one direction, and prevents the liquid from flowing backwards.
In fig. 6, the left end a of the dotted line is inserted into the groove 3 and the right end B is inserted into the port 9. The magnetic block 7 is located directly below the working electrode circle, which is shown filled with an inclined dotted line in fig. 6.
The specific embodiments described herein are merely illustrative of the spirit of the invention. Various modifications, additions and substitutions for the specific embodiments described herein may be made by those skilled in the art without departing from the spirit of the invention or exceeding the scope of the invention as defined in the accompanying claims.
Although the terms of the base 1, the insertion groove 2, the groove 3, the liquid inlet channel 4, the liquid outlet channel 5, the bottom plate 6, the magnetic block 7, the detection table 8, the port 9, the connection end 10, the snap-in groove 11, the snap-in block 12, the sealing ring 13, the slot 14, the fixing piece 15, the insertion hole 16, the one-way valve 17, the liquid outlet pipe 18, the water stop clip 19, the liquid inlet pipe 20, the working electrode 21, the counter electrode 22, the reference electrode 23, etc. are used more frequently, the possibility of using other terms is not excluded. These terms are used merely to more conveniently describe and explain the nature of the present invention; they are to be construed in a manner that is inconsistent with the spirit of the invention.
Claims (7)
1. The utility model provides a can be used to prepare electrochemical sensor working electrode's magnetic means, a serial communication port, including base (1), power supply scraps of paper one end is seted up to base (1) one side and is inserted insertion groove (2), base (1) be provided with recess (3), base (1) still be provided with inlet channel (4) and liquid outlet channel (5) with recess (3) intercommunication, inlet channel (4) be used for the entering of eluant, liquid outlet channel (5) be used for the outflow of eluant, the bottom of base (1) is provided with bottom plate (6), bottom plate (6) on be provided with magnetic path (7), magnetic path (7) be located under electrode scraps of paper carbon electrode.
2. The magnetic device for preparing the working electrode of the electrochemical sensor according to claim 1, further comprising a detection table (8), wherein the detection table (8) is provided with a port (9), the port (9) is used for inserting an electrode paper sheet at one end outside the insertion slot (2), and the detection table (8) is used for detecting electrochemical information of the electrode paper sheet.
3. The magnetic device for preparing the working electrode of the electrochemical sensor according to claim 2, wherein the port (9) is provided with a connecting end (10), the connecting end (10) is respectively connected with the working electrode (21), the reference electrode (23) and the counter electrode (22) in a contact manner, and the connecting end (10) is used for being externally connected with a computer.
4. The magnetic device for preparing the working electrode of the electrochemical sensor according to claim 2, wherein the test platform (8) is provided with a snap-in groove (11), the base (1) is provided with a snap-in block (12), and when the end of the electrode paper sheet outside the insertion groove (2) is inserted into the port (9), the snap-in block (12) is inserted into the snap-in groove (11).
5. The magnetic device for preparing a working electrode of an electrochemical sensor according to claim 1, characterized in that the peripheral wall of the insertion groove (2) is provided with a sealing ring (13).
6. The magnetic device for preparing a working electrode of an electrochemical sensor according to claim 1, wherein the base plate (6) is detachably connected to the base (1).
7. The magnetic device for preparing the working electrode of the electrochemical sensor according to claim 6, wherein the base (1) is provided with a slot (14) at the bottom for inserting the bottom plate (6), the base (1) is provided with a fixing plate (15) for fixing the base (1), the two ends of the base (1) are provided with insertion holes (16), and the fixing plate (15) is inserted into the insertion holes (16) to fix the bottom plate (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920884512.7U CN211263284U (en) | 2019-06-12 | 2019-06-12 | Magnetic device for preparing working electrode of electrochemical sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920884512.7U CN211263284U (en) | 2019-06-12 | 2019-06-12 | Magnetic device for preparing working electrode of electrochemical sensor |
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| Publication Number | Publication Date |
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| CN211263284U true CN211263284U (en) | 2020-08-14 |
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| CN201920884512.7U Expired - Fee Related CN211263284U (en) | 2019-06-12 | 2019-06-12 | Magnetic device for preparing working electrode of electrochemical sensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683976A (en) * | 2020-12-24 | 2021-04-20 | 南京云优生物科技有限公司 | Electrochemical magnetic biosensor and preparation method thereof |
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2019
- 2019-06-12 CN CN201920884512.7U patent/CN211263284U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683976A (en) * | 2020-12-24 | 2021-04-20 | 南京云优生物科技有限公司 | Electrochemical magnetic biosensor and preparation method thereof |
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