CN206756840U - A kind of preoperative hepatitis A, five indexes of hepatitis b and blood sieve four detection means - Google Patents

Abstract

The utility model provides a kind of preoperative hepatitis A, five indexes of hepatitis b and blood and sieves four detection means, including box body；The reservoir to match with its size is provided with the test flume, the opening of the reservoir is provided with supporting plate；The supporting plate is provided with manhole, and the hole inwall of the manhole is provided with concentric annular protuberance, and the core filter disc is pasted onto the upper surface of protuberance；The funnel is located at the lower section of core filter disc；Vavuum pump and battery are provided with the equipment slots；The exhaust tube passes through the top that jack is extend into inside reservoir；The nitrocellulose filter is circle, and nature controlling line and several test points are coated with the center on the surface of nitrocellulose filter.The detection means, simple in construction, high sensitivity, detection time is short, can detect hepatitis A, five indexes of hepatitis b and blood simultaneously and sieve four 9 kinds of infection sexually transmitted diseases, and help is provided for especially preoperative detection on hospital clinical.

Description

A kind of preoperative hepatitis A, five indexes of hepatitis b and blood sieve four detection means

Technical field

The utility model is applied to preoperative detection, and it is fast to ooze worry method (DIGFA) colloidal gold technique especially with spot immune Infectious disease and blood sieve four detection means before fast detection technique.

Background technology

In order to take precautions against medical-risk, operation safety is ensured, before surgery, hospital typically will carry out conventional inspection to patient to it Look into, its inspection project, which generally comprises five indexes of hepatitis b, hepatitis A and blood, which sieves four, (mainly includes anti-hepatitis b form original, hepatitis, syphilis and Chinese mugwort Grow disease) detection, operation risk is reduced, doctor is carried out the precautionary measures in advance to patient and is likely to occur the preparation of problem.

Infectious disease (Infectious Diseases) is can be in person to person, animal and animal as caused by various pathogen Or a kind of disease mutually propagated between humans and animals.Infectious disease divides rapid infectious disease and chronic infection sexually transmitted disease again, quickly It is largely microorganism in the pathogen of infectious disease, fraction is parasite, and parasite causer is also known as parasitic disease.With biography Broadcast that speed is fast, disseminates the features such as area is wide, harmfulness is big, propagated by air, can, this kind of infectious disease is typically easier to be sent out It is existing, mostly also can timely it be treated, the state of an illness is easily controlled, and reduces probability of infection.And chronic infection sexually transmitted disease is main Four (including hepatitis B mark antigen, hepatitis, syphilis and AIDS) etc. are sieved including hepatitis A, five indexes of hepatitis b blood, these diseases typically have There is rest period in incubation period, its incubation period or rest period are generally some months, several years or even decades, will not enter by air Row infects, and is usually infectious infection, can just be infected by the directly contact such as blood, saliva, preclinical patient is typically seldom Show illness, be not easy to find, also often it is ignored, often cannot timely and effectively treat, clinically typically by serum, Blood plasma etc. is detected, and only needs to treat in time for general patient, but for the patient to be undergone surgery, such as Fruit doctor does not know that patient with infection sexually transmitted disease, cannot carry out the reply of emergency situations in sufficiently preparation, art in the preoperative Measure and the prediction of postoperative risk, there is threat to doctor and patient, it is therefore, preoperative that patient is carried out to infect sexually transmitted disease detection It is most important.

Infection sexually transmitted disease five indexes of hepatitis b, hepatitis A and blood, which sieve four, (mainly includes anti-hepatitis b form original, hepatitis, syphilis and Chinese mugwort Grow disease) detection method mainly have conventional detection, colloidal gold immunochromatographimethod technology for detection, colloidal gold dot immunity percolation method (DIGFA), molecular detecting method etc., typically take the methods of conventional detection, Molecular Detection more, it is necessary to professional operates, Higher is required to staff quality；Colloidal gold immunochromatographimethod detection method has the advantages that high sensitivity, detection time is short, is to face Preferably a kind of detection method of preoperative detection on bed.But traditional colloidal gold immunochromatographimethod technology can only typically detect therein A kind of, two kinds or an intermediate item detection, is mostly single deck tape-recorder or connection card, the error caused by outside cause easily occurs during detection, Influence the accuracy of testing result；Colloidal gold dot immunity percolation method (DIGFA) is set up on the basis of ELISA, no Reaction of the enzyme to substrate is needed, it is easier, quick, but existing at present ooze considers detection kit typically by percolation box, nitre Acid cellulose film, absorbent material composition, often require higher to the water imbibition of absorbent material, water-absorption material during detection below film Material mainly siphons away useless albumen or impurity, but because the water imbibition of absorbent material is difficult to control, as serum, blood plasma etc. are relatively viscous Property sample be not easy to be oozed worry, can also produce the interference such as color to background, and as when the liquid flows such as urine try pattern detection effectively into Point easily being oozed worry walks, and reduces the reaction time of sample and antibody (or antigen) on film, influences Detection results, make doctor to patient Disease condition judgement produce interference.

Utility model content

The utility model is directed to above-mentioned problem and improved, there is provided a kind of preoperative hepatitis A, five indexes of hepatitis b Four spot immunes are sieved with blood and ooze worry method (DIGFA) collaurum detection means, and spot gold is immunized and oozes worry method and small-sized suction filtration dress Put and be combined, realize high flux quick detection, have it is simple in construction, high sensitivity, detection time is short, can detect simultaneously hepatitis A, Five indexes of hepatitis b and blood sieve four 9 kinds of infection sexually transmitted diseases, provide help for especially preoperative detection on hospital clinical, join for doctor Examine, there is higher reference value.

To reach above-mentioned purpose, what the technical solution of the utility model was realized in：

A kind of preoperative hepatitis A, five indexes of hepatitis b and blood sieve four detection means, including the filter of box body, supporting plate, funnel, core Piece, reservoir, vavuum pump and battery；

The inside of the box body is divided into trial zone, battery limits and medicine area from left to right, and the trial zone is provided with examination Check of foundation subsoil, the battery limits are provided with equipment slots, the medicine area be provided with golden standard liquid freeze-dried powder reagent bottle groove, cleaning solution reagent bottle groove and Nitrocellulose filter groove；

The reservoir to match with its size is provided with the test flume, the opening of the reservoir is provided with supporting plate, The size of the supporting plate and the opening size of reservoir match；The supporting plate is provided with manhole, described circular logical The hole inwall in hole is provided with concentric annular protuberance, and the core filter disc is pasted onto the upper surface of protuberance；The leakage Bucket is connected positioned at the lower section of core filter disc with supporting plate；

Vavuum pump and battery are provided with the equipment slots, the vavuum pump electrically connects with battery；The pumping of the vavuum pump Exhaust tube is connected on mouth, jack is additionally provided with the supporting plate, the exhaust tube extend into upper inside reservoir through jack Side；

Nitrocellulose filter is placed in the nitrocellulose filter groove, the nitrocellulose filter is circle, and nitric acid is fine Tie up and nature controlling line (C lines) and several test points (T lines) are coated with the center on the surface of plain film, the test point is in the form of an array Arrangement.

Further, the manhole is 4, and is uniformly distributed on the supporting plate, the manhole it is a diameter of 17mm。

Further, the external diameter of the protuberance is 17mm, internal diameter 15mm.

Further, a diameter of 17mm of the core filter disc, the internal package of the core filter disc have core, the core For diameter 15mm thickness 4mm cylindrical structure, the core and core filter disc are concentric.

Further, a diameter of 17mm of the nitrocellulose filter, the test point are hepatitis A, five indexes of hepatitis b, hepatitis, plum 3 × 3 matrix dots that the corresponding antibodies or antigen of poison and AIDS are formed, the test point are the round dot that diameter is less than or equal to 2mm, And the distance between each test point is 2mm；The nature controlling line is sheep anti-mouse igg；The test point and nature controlling line are located at diameter In border circular areas no more than 15mm, and the center of the border circular areas and the center of nitrocellulose filter are concentric.

Further, the bottom of the reservoir is provided with liquid overflow pipe, and the liquid overflow pipe is provided with valve.

Further, the bottom of the reservoir is ramped shaped.

Further, the porch of the exhaust tube is provided with baffle plate, has gap between the baffle plate and entrance.

Further, closure is additionally provided with the manhole, the edge of the closure is provided with sealing ring.

Further, the exhaust nozzle on the vavuum pump extend out to the outside of box body.

Further, the thickness of the supporting plate is 6mm.

Further, the core includes hydrophobic material；The surface of the core filter disc is smooth and with some ultra-fine more micro- Hole, aperture are 1.5-2.5 μm.

Further, the present apparatus is also equipped with detection project tag card, and tag card indicates project name by detection ordering, is easy to make User detects observation.

Relative to prior art, detection means described in the utility model has the advantage that：

On nitrocellulose filter with the corresponding antibody of the different infection sexually transmitted disease detection project of microarray formats point sample or Antigen, while detect hepatitis A, five indexes of hepatitis b and blood and sieve four (including anti-hepatitis b form original, hepatitis, syphilis and AIDS) 9 kinds of infection Sexually transmitted disease, convenient detection observation, makes doctor carry out the counter-measure of emergency situations and postoperative wind in preoperative adequate preparation, art Danger prediction, this clinically especially pre-operative patients infection sexually transmitted disease detection it is all most important, provide reference for doctor, With higher reference value.

Collaurum diafiltration diaphragm is combined by the detection means with Suction filtration device, has simple to operate, portable, sealing Good, controllability is strong, can repetitive cycling use, detection sample is filled with the antibody (or antigen) on nitrocellulose filter during detection Redundance is removed again after point haptoreaction, and 4 manholes and 4 funnels composition core filter passages, can be examined simultaneously in addition The disparity items of different samples is surveyed, realizes high flux quick detection.Vavuum pump is not direct to be connected with funnel, but is connected to liquid storage In groove, it can effectively prevent from, because pressure crosses the damage of senior general's nitrocellulose filter, influenceing testing result or testing result being inaccurate.

Brief description of the drawings

Form a part of accompanying drawing of the present utility model to be used for providing further understanding to of the present utility model, this practicality is new The schematic description and description of type is used to explain the utility model, does not form to improper restriction of the present utility model. In accompanying drawing：

Fig. 1 sieves the structure of four detection means for the preoperative hepatitis A described in the utility model embodiment, five indexes of hepatitis b and blood Schematic diagram；

Fig. 2 sieves the vertical view of four detection means for the preoperative hepatitis A described in the utility model embodiment, five indexes of hepatitis b and blood Figure；

Fig. 3 is the top view of the closure described in the utility model embodiment；

Fig. 4 is the side view of the closure described in the utility model embodiment；

Fig. 5 is the testing result display figure of the nitrocellulose filter described in the utility model embodiment；

Fig. 6 is the detection project tag card described in the utility model embodiment；

Fig. 7 is the partial enlarged drawing at the A of the detection means described in the utility model embodiment；

Fig. 8 is the top view of the protuberance described in the utility model embodiment.

Description of reference numerals：

1- box bodys；2- supporting plates；3- manholes；4- protuberances；5- funnels；6- core filter discs；7- reservoirs；8- discharges Pipe；9- valves；10- vavuum pumps；11- exhaust tubes；12- batteries；13- gold standard liquid freeze-dried powder reagent bottle grooves；14- cleaning solution reagent bottles Groove；15- nitrocellulose filter grooves；16- closures；17- sealing rings；18- exhaust nozzles；19- baffle plates.

Embodiment

It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the utility model can To be mutually combined.

In description of the present utility model, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " on ", " under ", The orientation or position relationship of the instruction such as "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are Based on orientation shown in the drawings or position relationship, it is for only for ease of description the utility model and simplifies description, rather than instruction Or imply that signified device or element must have specific orientation, with specific azimuth configuration and operation, therefore be not understood that For to limitation of the present utility model.In addition, term " first ", " second " etc. are only used for describing purpose, and it is not intended that instruction Or imply relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, " first ", " second " etc. are defined Feature can express or implicitly include one or more this feature.In description of the present utility model, unless separately It is described, " multiple " are meant that two or more.

, it is necessary to which explanation, unless otherwise clearly defined and limited, term " are pacified in description of the present utility model Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integratedly Connection；Can be mechanical connection or electrical connection；Can be joined directly together, can also be indirectly connected by intermediary, It can be the connection of two element internals.For the ordinary skill in the art, on being understood by concrete condition State concrete meaning of the term in the utility model.

Describe the utility model in detail below with reference to the accompanying drawings and in conjunction with the embodiments.

A kind of preoperative hepatitis A, five indexes of hepatitis b and blood sieve four detection means, including box body 1, supporting plate 2, funnel 5, core Filter disc 6, reservoir 7, vavuum pump 10 and battery 12；

The inside of the box body 1 is divided into trial zone, battery limits and medicine area from left to right, and the trial zone is provided with examination Check of foundation subsoil, the battery limits are provided with equipment slots, and the medicine area is provided with golden standard liquid freeze-dried powder reagent bottle groove 13, cleaning solution reagent bottle groove 14 and nitrocellulose filter groove 15, and golden standard liquid freeze-dried powder reagent bottle groove 13, cleaning solution reagent bottle groove 14 and nitrocellulose filter Groove 15 is arranged in a row by from rear to preceding order；

The reservoir 7 to match with its size is provided with the test flume, the opening of the reservoir 7 is provided with thick 6mm Supporting plate 2, the size and the opening size of reservoir 7 of the supporting plate 2 match；The supporting plate 2 is provided with 4 uniformly The manhole 3 of distribution, a diameter of 17mm of the manhole 3；The hole inwall of the manhole 3 is provided with concentric The protuberance 4 of annular, the external diameter of the protuberance 4 is 17mm, internal diameter 15mm；The core filter disc 6 is closely pasted onto prominent Go out the upper surface in portion 4, prevent gas leakage；A diameter of 17mm of the core filter disc 6, the internal package of the core filter disc 6 have sand Core, the core are diameter 15mm thickness 4mm cylindrical structure, and the core and core filter disc 6 are concentric, and the core includes thin Water material, making detection sample, (or antigen fully contacts, and is percolated by external force, can with the antibody on nitrocellulose filter Control property is strong, makes reaction more abundant, testing result is more accurate；The surface of the core filter disc 6 is smooth and with some ultra-fine more micro- Hole, aperture are 1.5-2.5 μm, can filter out larger bacterium of foreign protein and volume in sample etc., and nitrocellulose filter is risen To good supporting role, and core filter surface is smooth, will not damage nitrocellulose filter；The funnel 5 is located at sand The lower section of core filter disc 6, and be connected with supporting plate 2；The bottom of the reservoir 7 is provided with liquid overflow pipe 8, and the liquid overflow pipe 8 is provided with Valve 9, valve 9 can ensure the seal of whole device, due to the bad gas leakage effect testing result of seal when preventing from using, The bottom of the reservoir 7 is ramped shaped, is advantageous to the spilling of waste liquid；It is additionally provided with what is be matched therewith at the manhole 3 Closure 16, the edge of the closure 16 are provided with sealing ring 17, play better seal effect, and closure 16 can be with during detection By without using manhole 3 cover, avoid filter when to detect sample influence, and then influence testing result；

Vavuum pump 10 and battery 12 are provided with the equipment slots, the vavuum pump 10 electrically connects with battery 12；The vacuum Exhaust tube 11 is connected on the air exhaust nozzle of pump 10, is additionally provided with jack in the supporting plate 2, the exhaust tube 11 is extend into through jack Top inside reservoir 7；The porch of the exhaust tube 11 is provided with baffle plate 19, has gap between the baffle plate 19 and entrance, Baffle plate 19 prevents that solution excessively enters in vavuum pump 10 in reservoir, damages instrument；Exhaust nozzle 18 on the vavuum pump 10 is stretched Go out to the outside of box body 1；

Nitrocellulose filter is placed in the nitrocellulose filter groove 15, the nitrocellulose filter is shaped as diameter 17mm circle, and 1 nature controlling line (C lines) and 9 test points (T lines) are coated with the center on the surface of nitrocellulose filter, The test point is 3 × 3 matrix dots that the corresponding antibodies or antigen of hepatitis A, five indexes of hepatitis b, hepatitis, syphilis and AIDS are formed, The test point is the round dot that diameter is less than or equal to 2mm, and the distance between each test point is 2mm；The nature controlling line is sheep Anti- mouse IgG；The test point and nature controlling line be located at diameter no more than 15mm border circular areas in, and the center of the border circular areas with The center of nitrocellulose filter is concentric, and the detection zone of wherein test point composition accounts for the 2/3 of circle, and nature controlling line accounts for the 1/3 of circle, inspection Survey area and nature controlling line is at core, so can not only ensure the sealing of device but also not influence testing result.

The present apparatus is also equipped with detection project tag card, and tag card indicates project name by detection ordering, is easy to user to examine Survey observation.

Tell about the use process of the device in detail below in conjunction with the accompanying drawings：

The nitrocellulose filter of good seal and placed it in during detection, before taking-up on core filter disc 6, detection is then added dropwise Sample, it is fully reacted 30-60s, open vavuum pump 10, by the diafiltration of more waste liquids into reservoir 7；Then it is molten with buffer Liquid redissolves to be freezed made of the golden standard liquid for the antibody (or antigen) containing above-mentioned 9 kinds of detection projects being stored in brown ampoule bottle Powder, it is added dropwise on nitrocellulose filter, is filtered after reacting 30-60s；Then cleaning solution is added dropwise again, after reacting certain time, filters And result is observed, wherein cleaning solution can effectively prevent hepatitis A, five indexes of hepatitis b and blood from sieving the cross reaction between four.

After detection terminates, valve 9 is opened, the waste liquid in reservoir 7 is flowed out by liquid overflow pipe 8, close valve 9, ensured The seal of device.

Vavuum pump 10 of the present utility model is not connected directly between on funnel 5, so can effectively be prevented because pressure is excessive Nitrocellulose filter is damaged.

Hepatitis B surface antigen, hepatitis B surface antibody, hepatitis B e in hepatitis A, hepatitis, syphilis, AIDS and five indexes of hepatitis b resist It is former to be detected using the method that double antibody (or antigen) is sandwich or captures, when sample contains the corresponding antigen of above-mentioned detection When (or antibody), round dot is in aubergine at nature controlling line C line and T lines, is as a result the positive, as shown in Fig. 5 (a)；When in sample not During containing the corresponding antigen of above-mentioned detection (or antibody), there is aubergine band in nature controlling line C line, and T line round dots occur without aubergine, As a result it is feminine gender, as shown in Fig. 5 (b).

And hepatitis B e antibody and hepatitis B core antibody detect using collaurum competition law, its testing result just with above On the contrary, when detect contain hepatitis B e antibody and hepatitis B core antibody in sample when, there is aubergine band in nature controlling line C line, T lines circle Point occurs without aubergine, is as a result the positive, as shown in Fig. 5 (b)；Hepatitis B e antibody and hepatitis B core are not contained when detecting in sample During antibody, round dot is in aubergine at nature controlling line C line and T lines, is as a result feminine gender, as shown in Fig. 5 (a).

Regardless of detection method, if Quality Control C lines occur without aubergine band in detection, illustrate that operation is incorrect Or detection blocks to fail, such as Fig. 5 (shown in c).

The detection means is not limited only to infect the detection of sexually transmitted disease, the point sample method for coating on NC films, can examine simultaneously Multiple projects of a sample are surveyed, four passages that manhole and core filter disc form can detect different samples simultaneously not Same project, it can be achieved to detect while multisample, entry, and testing result is directly perceived, effecting reaction detection project situation, nothing Professional is needed, it is simple to operate, save detection time.Other method for coating of the present utility model is equally applicable to drugs, a huge sum of money The detection of pathogenic bacteria, antibiotic etc. in category, food, in addition, point-type, line style and other shapes belong to the utility model.

Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model Within the spirit and principle of utility model, any modification, equivalent substitution and improvements made etc., the utility model should be included in Protection domain within.

Claims (10)

1. a kind of preoperative hepatitis A, five indexes of hepatitis b and blood sieve four detection means, it is characterised in that：Including box body (1), supporting plate (2), funnel (5), core filter disc (6), reservoir (7), vavuum pump (10) and battery (12)；

The inside of the box body (1) is divided into trial zone, battery limits and medicine area from left to right, and the trial zone is provided with experiment Groove, the battery limits are provided with equipment slots, and the medicine area is provided with golden standard liquid freeze-dried powder reagent bottle groove (13), cleaning solution reagent bottle groove And nitrocellulose filter groove (15) (14)；

The reservoir (7) to match with its size is provided with the test flume, the opening of the reservoir (7) is provided with supporting plate (2), the size of the supporting plate (2) and the opening size of reservoir (7) match；The supporting plate (2) is provided with circular logical Hole (3), the hole inwall of the manhole (3) are provided with concentric annular protuberance (4), and the core filter disc (6) is viscous It is attached to the upper surface of protuberance (4)；The funnel (5) is located at the lower section of core filter disc (6), and is connected with supporting plate (2)；

Vavuum pump (10) and battery (12) are provided with the equipment slots, the vavuum pump (10) electrically connects with battery (12)；It is described Exhaust tube (11) is connected on the air exhaust nozzle of vavuum pump (10), is additionally provided with jack on the supporting plate (2), the exhaust tube (11) is worn Cross jack and extend into the internal top of reservoir (7)；

Nitrocellulose filter is placed in the nitrocellulose filter groove (15), the nitrocellulose filter is circle, and nitric acid is fine Tie up and nature controlling line and several test points are coated with the center on the surface of plain film, the test point is arranged in the form of an array.

2. detection means according to claim 1, it is characterised in that：The manhole (3) is 4, and is uniformly distributed In supporting plate (2), a diameter of 17mm of the manhole (3).

3. detection means according to claim 1, it is characterised in that：The external diameter of the protuberance (4) is 17mm, and internal diameter is 15mm。

4. detection means according to claim 1, it is characterised in that：A diameter of 17mm of the core filter disc (6), it is described The internal package of core filter disc (6) has a core, and the core is diameter 15mm thickness 4mm cylindrical structure, the core and sand Core filter disc (6) is concentric.

5. according to the detection means described in claim any one of 1-4, it is characterised in that：The nitrocellulose filter it is a diameter of 17mm, the test point are 3 × 3 squares that the corresponding antibodies or antigen of hepatitis A, five indexes of hepatitis b, hepatitis, syphilis and AIDS are formed Lattice point, the test point is the round dot that diameter is less than or equal to 2mm, and the distance between each test point is 2mm；The nature controlling line For sheep anti-mouse igg；The test point and nature controlling line are located in border circular areas of the diameter no more than 15mm, and in the border circular areas The center of the heart and nitrocellulose filter is concentric.

6. detection means according to claim 1, it is characterised in that：The bottom of the reservoir (7) is provided with liquid overflow pipe (8), the liquid overflow pipe (8) is provided with valve (9).

7. detection means according to claim 1, it is characterised in that：The bottom of the reservoir (7) is ramped shaped.

8. detection means according to claim 1, it is characterised in that：The porch of the exhaust tube (11) is provided with baffle plate (19), the baffle plate (19) has gap between entrance.

9. detection means according to claim 1, it is characterised in that：Manhole (3) place is additionally provided with closure (16), the edge of the closure (16) is provided with sealing ring (17).

10. detection means according to claim 1, it is characterised in that：Exhaust nozzle (18) on the vavuum pump (10) is stretched Go out to the outside of box body (1).