CN101738475A - Hepatitis E virus antibody detection kit and preparation method thereof - Google Patents
Hepatitis E virus antibody detection kit and preparation method thereof Download PDFInfo
- Publication number
- CN101738475A CN101738475A CN201010011330A CN201010011330A CN101738475A CN 101738475 A CN101738475 A CN 101738475A CN 201010011330 A CN201010011330 A CN 201010011330A CN 201010011330 A CN201010011330 A CN 201010011330A CN 101738475 A CN101738475 A CN 101738475A
- Authority
- CN
- China
- Prior art keywords
- gold mark
- preparation
- hepatitis
- kit
- box
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 66
- 241000724675 Hepatitis E virus Species 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims description 43
- 239000010931 gold Substances 0.000 claims abstract description 98
- 229910052737 gold Inorganic materials 0.000 claims abstract description 98
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 91
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 64
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 64
- 239000012530 fluid Substances 0.000 claims abstract description 54
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 238000003908 quality control method Methods 0.000 claims abstract description 41
- 238000005406 washing Methods 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000000427 antigen Substances 0.000 claims abstract description 21
- 102000036639 antigens Human genes 0.000 claims abstract description 21
- 108091007433 antigens Proteins 0.000 claims abstract description 21
- 239000000243 solution Substances 0.000 claims description 48
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 44
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 36
- 239000012141 concentrate Substances 0.000 claims description 28
- 238000010790 dilution Methods 0.000 claims description 24
- 239000012895 dilution Substances 0.000 claims description 24
- 239000012153 distilled water Substances 0.000 claims description 24
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 22
- 229940098773 bovine serum albumin Drugs 0.000 claims description 22
- 239000011780 sodium chloride Substances 0.000 claims description 22
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 22
- 241001494479 Pecora Species 0.000 claims description 20
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- 230000000274 adsorptive effect Effects 0.000 claims description 12
- 239000011259 mixed solution Substances 0.000 claims description 12
- 239000005030 aluminium foil Substances 0.000 claims description 11
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 150000002343 gold Chemical class 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000006193 liquid solution Substances 0.000 claims description 6
- 229910052708 sodium Inorganic materials 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 abstract description 58
- 238000006243 chemical reaction Methods 0.000 abstract description 38
- 210000002966 serum Anatomy 0.000 abstract description 14
- 238000012360 testing method Methods 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 241000283707 Capra Species 0.000 abstract 1
- 239000012528 membrane Substances 0.000 description 20
- 230000015572 biosynthetic process Effects 0.000 description 8
- 210000004369 blood Anatomy 0.000 description 8
- 239000008280 blood Substances 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 8
- 238000003745 diagnosis Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 239000003146 anticoagulant agent Substances 0.000 description 4
- 229940127219 anticoagulant drug Drugs 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 238000009666 routine test Methods 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 201000010284 hepatitis E Diseases 0.000 description 2
- 239000003547 immunosorbent Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010019786 Hepatitis non-A non-B Diseases 0.000 description 1
- 206010019799 Hepatitis viral Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 201000001862 viral hepatitis Diseases 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a hepatitis E virus antibody detection kit. The kit comprises a detection kit, a gold label working fluid bottle containing gold label working fluid and a washing liquid bottle containing washing liquid, wherein the detection kit comprises a detection kit body and a kit cover; the inner side of the kit cover is provided with a cellulose nitrate film; the kit cover corresponding to a position of the cellulose nitrate film is provided with a reaction hole; a water absorption pad is arranged in the kit body of the detection kit; the cellulose nitrate film is provided with a detection point and a quality control point; the detection point is coated with an HEV antigen; and the quality control point is coated with a goat anti-mouse antibody. The hepatitis E virus antibody can be diagnosed rapidly only by dripping serum to be test onto the cellulose nitrate film of the detection kit, so the kit has the characteristics of simple and convenient use and high detection speed. No apparatus is needed in clinical examination, so the effects on a result caused by various factors in an experimental process are prevented and the labor intensity of operators is relieved.
Description
Technical field
The present invention relates to a kind of hepatitis E virus antibody detection kit and preparation method thereof, belong to biological technical field.
Background technology
Hepatitis E is called intestinal transmitted non-A non-B hepatitis in the past.Hepatitis E virus (HEV) is mainly invaded liver, duplicates in liver cell, and this virus causes hepatocellular inflammation or necrosis by to hepatocellular coup injury and immunopathogenesis effect, shows as acute viral hepatitis clinically.Hepatitis E virus is mainly propagated through excrement-mouth approach.After the people infects hepatitis E virus, can show as clinical type and subclinical type and infect.This two classes patient all can discharge hepatitis E virus with ight soil, thus polluted source, food and surrounding environment and propagate.The detection of hepatitis E virus can be used as and cuts off the Hepatitis E route of transmission is main comprehensive preventive measure, wards off disease and propagates in the crowd.
The hepatitis E virus detection method mainly contains immunoelectron microscope at present, immunofluorescence technique, and enzyme linked immunological absorption reagent, albumen is inhaled seal test, RT-polymerase chain reaction method (RT-PCR).
The laboratory diagnostic method that HEV infects mainly contains Serological testing and Protocols in Molecular Biology, method commonly used has complement fixation test (CFT) and euzymelinked immunosorbent assay (ELISA) etc., the composition that complement fixation test (CFT) participates in reaction is many, the influence factor complexity, operation steps is complicated and requirement is very strict, occurs mistake easily; Euzymelinked immunosorbent assay (ELISA) complex operation, poor repeatability, required time are grown, are cost an arm and a leg, and need professional's operation experiments, have limited its application to a certain extent.
Summary of the invention
The problem to be solved in the present invention is at the deficiencies in the prior art, a kind of hepatitis E virus antibody detection kit that is applicable to clinical assistant diagnosis and discriminating and preparation method thereof is provided, and this kit has advantage simple in structure, easy to use, that detection speed is fast.
For overcoming the above problems, technical scheme of the present invention is as follows: hepatitis E virus antibody detection kit is characterized in that: described kit comprises:
Detect box, comprise and detect box box body and lid, the inboard of lid is provided with nitrocellulose filter, and the lid corresponding with the cellulose nitrate film location is provided with reacting hole, detects in the box box body and is provided with adsorptive pads, and nitrocellulose filter has been provided with check point and Quality Control point;
Gold mark working fluid bottle fills gold mark working fluid, and this gold mark working fluid is mixed by gold mark concentrate and dilution; With
Washing liquid bottle fills cleansing solution, and this cleansing solution comprises trishydroxymethylaminomethane, sodium chloride, bovine serum albumin(BSA) and hydrochloric acid;
Wherein, check point is coated with HEV antigen, and the Quality Control point is coated with sheep anti-mouse antibody.
As further improvement in the technical proposal:
Described gold mark concentrate is 1 with the mixed volume ratio of dilution: 15-30.
Described gold mark concentrate is 1: 20 with the mixed volume ratio of dilution.
Described preparation method comprises the preparation that detects box, gold mark working fluid and cleansing solution;
Wherein, the preparation of gold mark working fluid may further comprise the steps:
A, preparation gold mark concentrate
Get the aqueous solution of chloraurate 100ml of 1-4%, be heated to boiling, add 0.5-1% citric acid three sodium solution 0.8ml, mixing treats that solution colour by yellowish → blueness → purple → complete transparent claret, continued to boil 10 minutes rapidly, stop heating, be cooled to room temperature;
In the solution that obtains, add the anti-human IgG monoclonal anti liquid solution 100 μ l of 1mg/ml, obtain mixed solution;
Then mixed solution is carried out centrifugal treating, abandoning supernatant obtains lower floor's gold mark concentrate, and is standby;
B, preparation dilution
The trishydroxymethylaminomethane of 121.1-363.3mg, the sodium chloride of 0.1-1mg and the bovine serum albumin(BSA) of 0.5-2.0mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, at last with hydrochloric acid with its pH regulator to 7.4, obtain dilution, preserve down at 2～8 ℃, standby;
C, dilution and gold mark concentrate are mixed in proportion, obtain gold mark working fluid, and be placed in the gold mark working fluid bottle.
The preparation of described cleansing solution may further comprise the steps:
The trishydroxymethylaminomethane of 121.1-242.2mg, the sodium chloride of 0.1-1mg and the bovine serum albumin(BSA) of 0.1-1mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, be 7.4 with hydrochloric acid with its pH regulator again, obtain cleansing solution, be placed in the Washing liquid bottle, place 2～8 ℃ to preserve down.
The preparation of described detection box may further comprise the steps:
At first at the check point bag of nitrocellulose filter by HEV antigen, at Quality Control point bag by sheep anti-mouse antibody;
Then lid, nitrocellulose filter, adsorptive pads and detection box box body are assembled and fastening according to order from top to down, be assembled into the detection box;
Be placed on humidity less than 40% environment dry 2 hours down with detecting box;
Be placed on dried detection box in the aluminium foil bag at last and seal.
Among the step b, the consumption of trishydroxymethylaminomethane is 242.2mg, and amount of sodium chloride is 0.9mg, and the consumption of bovine serum albumin(BSA) is 1mg.
The consumption of described trishydroxymethylaminomethane is 121.1mg, and amount of sodium chloride is 0.9mg, and the consumption of bovine serum albumin(BSA) is 0.5mg.
The present invention adopts above technical scheme, compared with prior art, have the following advantages: form by detecting box, gold mark working fluid bottle and Washing liquid bottle, simple in structure, only need test serum is splashed on the nitrocellulose filter that detects box, just can quick diagnosis hepatitis E virus specific antibody, use simple and convenient, detection speed is fast, clinical examination does not need any instrument, has avoided various factors in the experimentation to result's influence and alleviated labor intensity of operating personnel.
The invention will be further described below in conjunction with drawings and Examples:
Description of drawings
Accompanying drawing 1 is the structural representation of kit in the embodiment of the invention;
Accompanying drawing 2 is the structural representation that detects box in the embodiment of the invention;
Accompanying drawing 3 be in the accompanying drawing 2 A-A to cut-open view.
Among the figure,
1-gold mark working fluid bottle, the 2-Washing liquid bottle, the 3-lid, the 4-nitrocellulose filter, the 5-adsorptive pads, 6-detects box box body, 7-check point, 8-Quality Control point, 9-reacting hole, 10-aluminium foil bag.
Specific embodiment
Embodiment 1, and as shown in Figure 1, hepatitis E virus antibody detection kit comprises and detects box, gold mark working fluid bottle 1 and Washing liquid bottle 2, detects box and is placed in the aluminium foil bag 10.
As Fig. 2, shown in Figure 3, detect box and comprise detection box box body 6 and lid 3, the inboard of lid 3 is provided with nitrocellulose filter 4, the lid 3 corresponding with nitrocellulose filter 4 positions is provided with reacting hole 9, detect in the box box body 6 and be provided with adsorptive pads 5, nitrocellulose filter 4 is provided with check point 7 and Quality Control point 8, and check point 7 is coated with HEV antigen, and Quality Control point 8 is coated with sheep anti-mouse antibody.
Fill gold mark working fluid in the gold mark working fluid bottle 1, this gold mark working fluid is obtained through diluted by gold mark concentrate.
Fill cleansing solution in the Washing liquid bottle 2, this cleansing solution is obtained by trishydroxymethylaminomethane, sodium chloride, bovine serum albumin(BSA) and hydrochloric acid preparation.
This kit prepares by following steps:
(1) preparation of detection box
At first the check point 7 at nitrocellulose filter wraps by HEV antigen, wraps by sheep anti-mouse antibody at Quality Control point 8;
With lid 3, nitrocellulose filter 4, adsorptive pads 5 with detect box box body 6, be assembled into the detection box then according to from top to down order assembling and fastening;
Be placed on humidity less than 40% environment dry 2 hours down with detecting box;
At last dried detection box is placed in the aluminium foil bag 10 and seals;
(2) preparation of gold mark working fluid
A, preparation gold mark concentrate
Get 1% aqueous solution of chloraurate 100ml, be heated to boiling, add 0.5% citric acid three sodium solution 0.8ml, mixing treats that solution colour by yellowish → blueness → purple → complete transparent claret, continued to boil 10 minutes rapidly, stops heating, is cooled to room temperature;
In the solution that obtains, add the anti-human IgG monoclonal anti liquid solution 100 μ l of 1mg/ml, obtain mixed solution;
Then mixed solution is carried out centrifugal treating, abandoning supernatant obtains lower floor's gold mark concentrate, and is standby;
B, preparation dilution
The trishydroxymethylaminomethane of 121.1mg, the sodium chloride of 0.1mg and the bovine serum albumin(BSA) of 0.5mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, at last with hydrochloric acid with its pH regulator to 7.4, obtain dilution, preserve down at 2～8 ℃, standby;
C, dilution and gold mark concentrate are mixed by 15: 1 volume ratio, obtain gold mark working fluid, and be placed on golden marking in the working fluid bottle 1;
(3) preparing washing liquid
The trishydroxymethylaminomethane of 121.1mg, the sodium chloride of 0.1mg and the bovine serum albumin(BSA) of 0.1mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, be 7.4 with hydrochloric acid with its pH regulator again, obtain cleansing solution, be placed in the Washing liquid bottle, place 2～8 ℃ to preserve down;
(4) after posting label on gold mark working fluid bottle 1 and the Washing liquid bottle 2, be assembled into kit with detecting box.
The use of above kit, detection method
1, sample collection
Venous blood is placed totally, do not add in the container of anti-coagulants, leave standstill blood is shunk naturally, centrifuging and taking serum detects.
2, detect
A, taking-up kit, equilibrium at room temperature 20～30 minutes;
B, drip 1 of cleansing solution on nitrocellulose membrane 4, treat that liquid is moistening fully with nitrocellulose membrane 4;
C, add 150 μ l serum to be measured on nitrocellulose membrane 4, treat that liquid fully sucks;
D, 3 gold marks of dropping working fluids treat that liquid fully sucks on nitrocellulose membrane 4;
E, 3 cleansing solutions of dropping treat that liquid fully sucks back observations in 3 minutes on nitrocellulose membrane 4.
3, the result judges
Positive: if contain hepatitis E virus antibody in the tested sample, HEV specific antibody in the sample just combines with solid phase HEV antigen generation specificity on the nitrocellulose filter 4 and forms compound, other no related substances are then filtered, add gold mark mouse-anti human monoclonal antibodies, gold mark monoclonal antibody just combines with compound during filtration, can observe red reaction marking at check point 7.Simultaneously, the sheep anti-mouse antibody of bag quilt combines on gold mark mouse-anti human monoclonal antibodies and the Quality Control point 8, and at the red reaction marking of Quality Control point 8 formation, check point 7 has red reaction marking appearance, and this shows in the tested sample and contains hepatitis E virus antibody, positive result.
Negative: if do not contain hepatitis E virus antibody in the tested sample, gold mark mouse-anti human monoclonal antibodies in the reaction system can't form indirect antigen-antibody reaction with the HEV antigen of bag quilt, promptly at check point 7 redfree reaction markings, simultaneously, the sheep anti-mouse antibody of bag quilt combines on gold mark monoclonal antibody and the Quality Control point 8, in the red reaction marking of Quality Control point 8 formation, the appearance of check point 7 redfree reaction markings, this shows and does not contain hepatitis E virus antibody, negative result in the tested sample.
Invalid: as after the reaction,, to show that misoperation or reagent lost efficacy if Quality Control point does not develop the color.
Use the kit of the embodiment of the invention 1000 routine test specimens to be detected result such as following table:
From above detection method and testing result as can be seen, kit of the present invention only needs test serum is splashed on the nitrocellulose filter that detects box, with regard to energy quick diagnosis hepatitis E virus specific antibody, use simple and convenient, detection speed is fast, the coincidence rate height, clinical examination does not need any instrument, has avoided various factors in the experimentation to result's influence and alleviated labor intensity of operating personnel.
As Fig. 2, shown in Figure 3, detect box and comprise detection box box body 6 and lid 3, the inboard of lid 3 is provided with nitrocellulose filter 4, the lid 3 corresponding with nitrocellulose filter 4 positions is provided with reacting hole 9, detect in the box box body 6 and be provided with adsorptive pads 5, nitrocellulose filter 4 is provided with check point 7 and Quality Control point 8, and check point 7 is coated with HEV antigen, and Quality Control point 8 is coated with sheep anti-mouse antibody.
Fill gold mark working fluid in the gold mark working fluid bottle 1, this gold mark working fluid is obtained through diluted by gold mark concentrate.
Fill cleansing solution in the Washing liquid bottle 2, this cleansing solution is obtained by trishydroxymethylaminomethane, sodium chloride, bovine serum albumin(BSA) and hydrochloric acid preparation.
This kit prepares by following steps:
(1) preparation of detection box
At first the check point 7 at nitrocellulose filter wraps by HEV antigen, wraps by sheep anti-mouse antibody at Quality Control point 8;
With lid 3, nitrocellulose filter 4, adsorptive pads 5 with detect box box body 6, be assembled into the detection box then according to from top to down order assembling and fastening;
Be placed on humidity less than 40% environment dry 2 hours down with detecting box;
At last dried detection box is placed in the aluminium foil bag 10 and seals;
(2) preparation of gold mark working fluid
A, preparation gold mark concentrate
Get 2% aqueous solution of chloraurate 100ml, be heated to boiling, add 0.7% citric acid three sodium solution 0.8ml, mixing treats that solution colour by yellowish → blueness → purple → complete transparent claret, continued to boil 10 minutes rapidly, stops heating, is cooled to room temperature;
In the solution that obtains, add the anti-human IgG monoclonal anti liquid solution 100 μ l of 1mg/ml, obtain mixed solution;
Then mixed solution is carried out centrifugal treating, abandoning supernatant obtains lower floor's gold mark concentrate, and is standby;
B, preparation dilution
The trishydroxymethylaminomethane of 363.3mg, the sodium chloride of 0.5mg and the bovine serum albumin(BSA) of 1.5mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, at last with hydrochloric acid with its pH regulator to 7.4, obtain dilution, preserve down at 2～8 ℃, standby;
C, dilution and gold mark concentrate are mixed by 20: 1 volume ratio, obtain gold mark working fluid, and be placed on golden marking in the working fluid bottle 1;
(3) preparing washing liquid
The trishydroxymethylaminomethane of 242.2mg, the sodium chloride of 0.5mg and the bovine serum albumin(BSA) of 0.7mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, be 7.4 with hydrochloric acid with its pH regulator again, obtain cleansing solution, be placed in the Washing liquid bottle, place 2～8 ℃ to preserve down;
(4) after posting label on gold mark working fluid bottle 1 and the Washing liquid bottle 2, be assembled into kit with detecting box.
The use of above kit, detection method
1, sample collection
Venous blood is placed totally, do not add in the container of anti-coagulants, leave standstill blood is shunk naturally, centrifuging and taking serum detects.
2, detect
A, taking-up kit, equilibrium at room temperature 20～30 minutes;
B, drip 1 of cleansing solution on nitrocellulose membrane 4, treat that liquid is moistening fully with nitrocellulose membrane 4;
C, add 150 μ l serum to be measured on nitrocellulose membrane 4, treat that liquid fully sucks;
D, 3 gold marks of dropping working fluids treat that liquid fully sucks on nitrocellulose membrane 4;
E, 3 cleansing solutions of dropping treat that liquid fully sucks back observations in 3 minutes on nitrocellulose membrane 4.
3, the result judges
Positive: if contain hepatitis E virus antibody in the tested sample, HEV specific antibody in the sample just combines with solid phase HEV antigen generation specificity on the nitrocellulose filter 4 and forms compound, other no related substances are then filtered, add gold mark mouse-anti human monoclonal antibodies, gold mark monoclonal antibody just combines with compound during filtration, can observe red reaction marking at check point 7.Simultaneously, the sheep anti-mouse antibody of bag quilt combines on gold mark mouse-anti human monoclonal antibodies and the Quality Control point 8, and at the red reaction marking of Quality Control point 8 formation, check point 7 has red reaction marking appearance, and this shows in the tested sample and contains hepatitis E virus antibody, positive result.
Negative: if do not contain hepatitis E virus antibody in the tested sample, gold mark mouse-anti human monoclonal antibodies in the reaction system can't form indirect antigen-antibody reaction with the HEV antigen of bag quilt, promptly at check point 7 redfree reaction markings, simultaneously, the sheep anti-mouse antibody of bag quilt combines on gold mark monoclonal antibody and the Quality Control point 8, in the red reaction marking of Quality Control point 8 formation, the appearance of check point 7 redfree reaction markings, this shows and does not contain hepatitis E virus antibody, negative result in the tested sample.
Invalid: as after the reaction,, to show that misoperation or reagent lost efficacy if Quality Control point does not develop the color.
Use the kit of the embodiment of the invention 1000 routine test specimens to be detected result such as following table:
From above detection method and testing result as can be seen, kit of the present invention only needs test serum is splashed on the nitrocellulose filter that detects box, with regard to energy quick diagnosis hepatitis E virus specific antibody, use simple and convenient, detection speed is fast, the coincidence rate height, clinical examination does not need any instrument, has avoided various factors in the experimentation to result's influence and alleviated labor intensity of operating personnel.
As Fig. 2, shown in Figure 3, detect box and comprise detection box box body 6 and lid 3, the inboard of lid 3 is provided with nitrocellulose filter 4, the lid 3 corresponding with nitrocellulose filter 4 positions is provided with reacting hole 9, detect in the box box body 6 and be provided with adsorptive pads 5, nitrocellulose filter 4 is provided with check point 7 and Quality Control point 8, and check point 7 is coated with HEV antigen, and Quality Control point 8 is coated with sheep anti-mouse antibody.
Fill gold mark working fluid in the gold mark working fluid bottle 1, this gold mark working fluid is obtained through diluted by gold mark concentrate.
Fill cleansing solution in the Washing liquid bottle 2, this cleansing solution is obtained by trishydroxymethylaminomethane, sodium chloride, bovine serum albumin(BSA) and hydrochloric acid preparation.
This kit prepares by following steps:
(1) preparation of detection box
At first the check point 7 at nitrocellulose filter wraps by HEV antigen, wraps by sheep anti-mouse antibody at Quality Control point 8;
With lid 3, nitrocellulose filter 4, adsorptive pads 5 with detect box box body 6, be assembled into the detection box then according to from top to down order assembling and fastening;
Be placed on humidity less than 40% environment dry 2 hours down with detecting box;
At last dried detection box is placed in the aluminium foil bag 10 and seals;
(2) preparation of gold mark working fluid
A, preparation gold mark concentrate
Get 3% aqueous solution of chloraurate 100ml, be heated to boiling, add 0.8% citric acid three sodium solution 0.8ml, mixing treats that solution colour by yellowish → blueness → purple → complete transparent claret, continued to boil 10 minutes rapidly, stops heating, is cooled to room temperature;
In the solution that obtains, add the anti-human IgG monoclonal anti liquid solution 100 μ l of 1mg/ml, obtain mixed solution;
Then mixed solution is carried out centrifugal treating, abandoning supernatant obtains lower floor's gold mark concentrate, and is standby;
B, preparation dilution
The trishydroxymethylaminomethane of 242.2mg, the sodium chloride of 0.9mg and the bovine serum albumin(BSA) of 1.0mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, at last with hydrochloric acid with its pH regulator to 7.4, obtain dilution, preserve down at 2～8 ℃, standby;
C, dilution and gold mark concentrate are mixed by 25: 1 volume ratio, obtain gold mark working fluid, and be placed on golden marking in the working fluid bottle 1;
(3) preparing washing liquid
The trishydroxymethylaminomethane of 121.1mg, the sodium chloride of 0.9mg and the bovine serum albumin(BSA) of 0.5mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, be 7.4 with hydrochloric acid with its pH regulator again, obtain cleansing solution, be placed in the Washing liquid bottle, place 2～8 ℃ to preserve down;
(4) after posting label on gold mark working fluid bottle 1 and the Washing liquid bottle 2, be assembled into kit with detecting box.
The use of above kit, detection method
1, sample collection
Venous blood is placed totally, do not add in the container of anti-coagulants, leave standstill blood is shunk naturally, centrifuging and taking serum detects.
2, detect
A, taking-up kit, equilibrium at room temperature 20～30 minutes;
B, drip 1 of cleansing solution on nitrocellulose membrane 4, treat that liquid is moistening fully with nitrocellulose membrane 4;
C, add 150 μ l serum to be measured on nitrocellulose membrane 4, treat that liquid fully sucks;
D, 3 gold marks of dropping working fluids treat that liquid fully sucks on nitrocellulose membrane 4;
E, 3 cleansing solutions of dropping treat that liquid fully sucks back observations in 3 minutes on nitrocellulose membrane 4.
3, the result judges
Positive: if contain hepatitis E virus antibody in the tested sample, HEV specific antibody in the sample just combines with solid phase HEV antigen generation specificity on the nitrocellulose filter 4 and forms compound, other no related substances are then filtered, add gold mark mouse-anti human monoclonal antibodies, gold mark monoclonal antibody just combines with compound during filtration, can observe red reaction marking at check point 7.Simultaneously, the sheep anti-mouse antibody of bag quilt combines on gold mark mouse-anti human monoclonal antibodies and the Quality Control point 8, and at the red reaction marking of Quality Control point 8 formation, check point 7 has red reaction marking appearance, and this shows in the tested sample and contains hepatitis E virus antibody, positive result.
Negative: if do not contain hepatitis E virus antibody in the tested sample, gold mark mouse-anti human monoclonal antibodies in the reaction system can't form indirect antigen-antibody reaction with the HEV antigen of bag quilt, promptly at check point 7 redfree reaction markings, simultaneously, the sheep anti-mouse antibody of bag quilt combines on gold mark monoclonal antibody and the Quality Control point 8, in the red reaction marking of Quality Control point 8 formation, the appearance of check point 7 redfree reaction markings, this shows and does not contain hepatitis E virus antibody, negative result in the tested sample.
Invalid: as after the reaction,, to show that misoperation or reagent lost efficacy if Quality Control point does not develop the color.
Use the kit of the embodiment of the invention 1000 routine test specimens to be detected result such as following table:
From above detection method and testing result as can be seen, kit of the present invention only needs test serum is splashed on the nitrocellulose filter that detects box, with regard to energy quick diagnosis hepatitis E virus specific antibody, use simple and convenient, detection speed is fast, the coincidence rate height, clinical examination does not need any instrument, has avoided various factors in the experimentation to result's influence and alleviated labor intensity of operating personnel.
As Fig. 2, shown in Figure 3, detect box and comprise detection box box body 6 and lid 3, the inboard of lid 3 is provided with nitrocellulose filter 4, the lid 3 corresponding with nitrocellulose filter 4 positions is provided with reacting hole 9, detect in the box box body 6 and be provided with adsorptive pads 5, nitrocellulose filter 4 is provided with check point 7 and Quality Control point 8, and check point 7 is coated with HEV antigen, and Quality Control point 8 is coated with sheep anti-mouse antibody.
Fill gold mark working fluid in the gold mark working fluid bottle 1, this gold mark working fluid is obtained through diluted by gold mark concentrate.
Fill cleansing solution in the Washing liquid bottle 2, this cleansing solution is obtained by trishydroxymethylaminomethane, sodium chloride, bovine serum albumin(BSA) and hydrochloric acid preparation.
This kit prepares by following steps:
(1) preparation of detection box
At first the check point 7 at nitrocellulose filter wraps by HEV antigen, wraps by sheep anti-mouse antibody at Quality Control point 8;
With lid 3, nitrocellulose filter 4, adsorptive pads 5 with detect box box body 6, be assembled into the detection box then according to from top to down order assembling and fastening;
Be placed on humidity less than 40% environment dry 2 hours down with detecting box;
At last dried detection box is placed in the aluminium foil bag 10 and seals;
(2) preparation of gold mark working fluid
A, preparation gold mark concentrate
Get 4% aqueous solution of chloraurate 100ml, be heated to boiling, add 1.0% citric acid three sodium solution 0.8ml, mixing treats that solution colour by yellowish → blueness → purple → complete transparent claret, continued to boil 10 minutes rapidly, stops heating, is cooled to room temperature;
In the solution that obtains, add the anti-human IgG monoclonal anti liquid solution 100 μ l of 1mg/ml, obtain mixed solution;
Then mixed solution is carried out centrifugal treating, abandoning supernatant obtains lower floor's gold mark concentrate, and is standby;
B, preparation dilution
The trishydroxymethylaminomethane of 242.2mg, the sodium chloride of 1mg and the bovine serum albumin(BSA) of 2mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, at last with hydrochloric acid with its pH regulator to 7.4, obtain dilution, preserve down at 2～8 ℃, standby;
C, dilution and gold mark concentrate are mixed by 30: 1 volume ratio, obtain gold mark working fluid, and be placed on golden marking in the working fluid bottle 1;
(3) preparing washing liquid
The trishydroxymethylaminomethane of 121.1mg, the sodium chloride of 1mg and the bovine serum albumin(BSA) of 1mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, be 7.4 with hydrochloric acid with its pH regulator again, obtain cleansing solution, be placed in the Washing liquid bottle, place 2～8 ℃ to preserve down;
(4) after posting label on gold mark working fluid bottle 1 and the Washing liquid bottle 2, be assembled into kit with detecting box.
The use of above kit, detection method
1, sample collection
Venous blood is placed totally, do not add in the container of anti-coagulants, leave standstill blood is shunk naturally, centrifuging and taking serum detects.
2, detect
A, taking-up kit, equilibrium at room temperature 20～30 minutes;
B, drip 1 of cleansing solution on nitrocellulose membrane 4, treat that liquid is moistening fully with nitrocellulose membrane 4;
C, add 150 μ l serum to be measured on nitrocellulose membrane 4, treat that liquid fully sucks;
D, 3 gold marks of dropping working fluids treat that liquid fully sucks on nitrocellulose membrane 4;
E, 3 cleansing solutions of dropping treat that liquid fully sucks back observations in 3 minutes on nitrocellulose membrane 4.
3, the result judges
Positive: if contain hepatitis E virus antibody in the tested sample, HEV specific antibody in the sample just combines with solid phase HEV antigen generation specificity on the nitrocellulose filter 4 and forms compound, other no related substances are then filtered, add gold mark mouse-anti human monoclonal antibodies, gold mark monoclonal antibody just combines with compound during filtration, can observe red reaction marking at check point 7.Simultaneously, the sheep anti-mouse antibody of bag quilt combines on gold mark mouse-anti human monoclonal antibodies and the Quality Control point 8, and at the red reaction marking of Quality Control point 8 formation, check point 7 has red reaction marking appearance, and this shows in the tested sample and contains hepatitis E virus antibody, positive result.
Negative: if do not contain hepatitis E virus antibody in the tested sample, gold mark mouse-anti human monoclonal antibodies in the reaction system can't form indirect antigen-antibody reaction with the HEV antigen of bag quilt, promptly at check point 7 redfree reaction markings, simultaneously, the sheep anti-mouse antibody of bag quilt combines on gold mark monoclonal antibody and the Quality Control point 8, in the red reaction marking of Quality Control point 8 formation, the appearance of check point 7 redfree reaction markings, this shows and does not contain hepatitis E virus antibody, negative result in the tested sample.
Invalid: as after the reaction,, to show that misoperation or reagent lost efficacy if Quality Control point does not develop the color.
Use the kit of the embodiment of the invention 1000 routine test specimens to be detected result such as following table:
From above detection method and testing result as can be seen, kit of the present invention only needs test serum is splashed on the nitrocellulose filter that detects box, with regard to energy quick diagnosis hepatitis E virus specific antibody, use simple and convenient, detection speed is fast, the coincidence rate height, clinical examination does not need any instrument, has avoided various factors in the experimentation to result's influence and alleviated labor intensity of operating personnel.
Claims (8)
1. hepatitis E virus antibody detection kit, it is characterized in that: described kit comprises:
Detect box, comprise and detect box box body (6) and lid (3), the inboard of lid (3) is provided with nitrocellulose filter (4), the lid (3) corresponding with nitrocellulose filter (4) position is provided with reacting hole (9), detect in the box box body (6) and be provided with adsorptive pads (5), nitrocellulose filter (4) has been provided with check point (7) and Quality Control point (8);
Gold mark working fluid bottle (1) fills gold mark working fluid, and this gold mark working fluid is mixed by gold mark concentrate and dilution; With
Washing liquid bottle (2) fills cleansing solution, and this cleansing solution comprises trishydroxymethylaminomethane, sodium chloride, bovine serum albumin(BSA) and hydrochloric acid;
Wherein, check point (7) is coated with HEV antigen, and Quality Control point (8) is coated with sheep anti-mouse antibody.
2. hepatitis E virus antibody detection kit as claimed in claim 1 is characterized in that: described gold mark concentrate is 1 with the mixed volume ratio of dilution: 15-30.
3. hepatitis E virus antibody detection kit as claimed in claim 2 is characterized in that: described gold mark concentrate is 1: 20 with the mixed volume ratio of dilution.
4. the preparation method of hepatitis E virus antibody detection kit is characterized in that: described preparation method comprises the preparation that detects box, gold mark working fluid and cleansing solution;
Wherein, the preparation of gold mark working fluid may further comprise the steps:
A, preparation gold mark concentrate
Get the aqueous solution of chloraurate 100ml of 1-4%, be heated to boiling, add 0.5-1% citric acid three sodium solution 0.8ml, mixing treats that solution colour by yellowish → blueness → purple → complete transparent claret, continued to boil 10 minutes rapidly, stop heating, be cooled to room temperature;
In the solution that obtains, add the anti-human IgG monoclonal anti liquid solution 100 μ l of 1mg/ml, obtain mixed solution;
Then mixed solution is carried out centrifugal treating, abandoning supernatant obtains lower floor's gold mark concentrate, and is standby;
B, preparation dilution
The trishydroxymethylaminomethane of 121.1-363.3mg, the sodium chloride of 0.1-1mg and the bovine serum albumin(BSA) of 0.5-2.0mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, at last with hydrochloric acid with its pH regulator to 7.4, obtain dilution, preserve down at 2～8 ℃, standby;
C, dilution and gold mark concentrate are mixed in proportion, obtain gold mark working fluid, and be placed in the gold mark working fluid bottle (1).
5. the preparation method of hepatitis E virus antibody detection kit as claimed in claim 4, it is characterized in that: the preparation of described cleansing solution may further comprise the steps:
The trishydroxymethylaminomethane of 121.1-242.2mg, the sodium chloride of 0.1-1mg and the bovine serum albumin(BSA) of 0.1-1mg are placed in the volumetric flask, add distilled water, jog makes it to dissolve fully, continue then to add distilled water to 100mL, be 7.4 with hydrochloric acid with its pH regulator again, obtain cleansing solution, be placed in the Washing liquid bottle (2), place 2～8 ℃ to preserve down.
6. the preparation method of hepatitis E virus antibody detection kit as claimed in claim 4, it is characterized in that: the preparation of described detection box may further comprise the steps:
At first the check point (7) at nitrocellulose filter wraps by HEV antigen, wraps by sheep anti-mouse antibody at Quality Control point (8);
Then lid (3), nitrocellulose filter (4), adsorptive pads (5) and detection box box body (6) are assembled and fastening according to order from top to down, be assembled into the detection box;
Be placed on humidity less than 40% environment dry 2 hours down with detecting box;
Be placed on dried detection box in the aluminium foil bag at last and seal.
7. the preparation method of hepatitis E virus antibody detection kit as claimed in claim 4, it is characterized in that: among the step b, the consumption of trishydroxymethylaminomethane is 242.2mg, and amount of sodium chloride is 0.9mg, and the consumption of bovine serum albumin(BSA) is 1mg.
8. the preparation method of hepatitis E virus antibody detection kit as claimed in claim 5, it is characterized in that: the consumption of described trishydroxymethylaminomethane is 121.1mg, and amount of sodium chloride is 0.9mg, and the consumption of bovine serum albumin(BSA) is 0.5mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010011330 CN101738475B (en) | 2010-01-11 | 2010-01-11 | Hepatitis E virus antibody detection kit and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010011330 CN101738475B (en) | 2010-01-11 | 2010-01-11 | Hepatitis E virus antibody detection kit and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101738475A true CN101738475A (en) | 2010-06-16 |
| CN101738475B CN101738475B (en) | 2013-04-17 |
Family
ID=42462215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010011330 Expired - Fee Related CN101738475B (en) | 2010-01-11 | 2010-01-11 | Hepatitis E virus antibody detection kit and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101738475B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288759A (en) * | 2011-07-29 | 2011-12-21 | 北京中检安泰诊断科技有限公司 | Hepatitis E virus (HEV) IgG antibody colloidal gold method detection kit and preparation method thereof |
| CN103033616A (en) * | 2012-12-24 | 2013-04-10 | 潍坊市康华生物技术有限公司 | Detection method of hemorrhagic fever with renal syndrome IgM antibodies and reagent kit |
| CN103420038A (en) * | 2012-05-17 | 2013-12-04 | 讯映光电股份有限公司 | Biological test block containing barrel with dehumidification capacity detection device |
| CN104090105A (en) * | 2014-07-10 | 2014-10-08 | 广州市丰华生物工程有限公司 | Method and kit for detecting hepatitis E virus (HEV) antibody and method for preparing kit |
| CN108303550A (en) * | 2018-02-09 | 2018-07-20 | 广东优尼德生物科技有限公司 | A kind of the spot gold diafiltration kit and its quantitative detecting method of detection adiponectin |
| CN110300893A (en) * | 2017-02-21 | 2019-10-01 | 硕腾服务有限责任公司 | Diagnostic test reader system |
| CN111208298A (en) * | 2020-02-11 | 2020-05-29 | 潍坊市康华生物技术有限公司 | 2019 novel coronavirus antibody detection kit and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1013638B (en) * | 1986-02-26 | 1991-08-28 | 海茵茨·乔治·伯斯 | Shower partition |
| CN1409111A (en) * | 2001-09-28 | 2003-04-09 | 上海数康生物科技有限公司 | Device for simultanously detecting multiple antigen and antibody associated with diseases |
| CN101051049A (en) * | 2007-05-11 | 2007-10-10 | 杨致亭 | Card type mycoplasma pneumiae anti-body detection reagent box |
-
2010
- 2010-01-11 CN CN 201010011330 patent/CN101738475B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102288759A (en) * | 2011-07-29 | 2011-12-21 | 北京中检安泰诊断科技有限公司 | Hepatitis E virus (HEV) IgG antibody colloidal gold method detection kit and preparation method thereof |
| CN103420038A (en) * | 2012-05-17 | 2013-12-04 | 讯映光电股份有限公司 | Biological test block containing barrel with dehumidification capacity detection device |
| CN103033616A (en) * | 2012-12-24 | 2013-04-10 | 潍坊市康华生物技术有限公司 | Detection method of hemorrhagic fever with renal syndrome IgM antibodies and reagent kit |
| CN103033616B (en) * | 2012-12-24 | 2015-02-11 | 潍坊市康华生物技术有限公司 | Detection method of hemorrhagic fever with renal syndrome IgM antibodies and reagent kit |
| CN104090105A (en) * | 2014-07-10 | 2014-10-08 | 广州市丰华生物工程有限公司 | Method and kit for detecting hepatitis E virus (HEV) antibody and method for preparing kit |
| CN110300893A (en) * | 2017-02-21 | 2019-10-01 | 硕腾服务有限责任公司 | Diagnostic test reader system |
| CN110300893B (en) * | 2017-02-21 | 2023-09-05 | 硕腾服务有限责任公司 | Diagnostic Test Reader System |
| CN108303550A (en) * | 2018-02-09 | 2018-07-20 | 广东优尼德生物科技有限公司 | A kind of the spot gold diafiltration kit and its quantitative detecting method of detection adiponectin |
| CN111208298A (en) * | 2020-02-11 | 2020-05-29 | 潍坊市康华生物技术有限公司 | 2019 novel coronavirus antibody detection kit and preparation method thereof |
| CN111208298B (en) * | 2020-02-11 | 2023-10-27 | 山东康华生物医疗科技股份有限公司 | 2019 novel coronavirus antibody detection kit and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101738475B (en) | 2013-04-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101738475B (en) | Hepatitis E virus antibody detection kit and preparation method thereof | |
| CN105004862B (en) | Reagent for high throughput combined detection of hepatitis c virus antigen-antibody | |
| CN111378018B (en) | Test strip for detecting novel coronavirus antibody and preparation method and application thereof | |
| CN201965131U (en) | Quick test paper of hepatitis A virus (HAV) IgM antibody | |
| CN104126120A (en) | Paper based diagnostic test | |
| CN108918866A (en) | A kind of marker of inflammation POCT combined detection kit suit | |
| CN106093383A (en) | Porcine reproductive and respiratory syndrome virus antibody ELISA diagnosis reagent kit and preparation method thereof | |
| CN105717308A (en) | Immunochromatography kit for fast and quantitatively detecting fecal lactoferrin | |
| CN101178404A (en) | Human immunodeficiency virus antibody chemiluminescence immune analyzing diagnose reagent box and method of producing the same | |
| CN105753981B (en) | The immune chromatography reagent kit of anti-human Respiratory Syncytial Virus(RSV) N protein antibody and the application antibody | |
| CN101762702A (en) | Hepatitis A virus antibody assay kit and preparation method thereof | |
| CN106908602A (en) | A kind of preparation method of plasmodium pf/pan Test papers | |
| CN106596968A (en) | Dot gold infiltration kit for detecting urine microalbumin, and application of dot gold infiltration kit | |
| CN1159580C (en) | Reagent for colloidal gold chromatographic analysis of SARS coronavirus antigen | |
| CN103033616B (en) | Detection method of hemorrhagic fever with renal syndrome IgM antibodies and reagent kit | |
| CN102692502B (en) | Rapid hepatitis A virus IgM antibody detection kit and preparation method thereof | |
| CN111796103A (en) | Colloidal gold test strip for detecting echinococcosis and preparation method thereof | |
| CN104391124B (en) | A kind of myoglobins detects the preparation method of reagent | |
| CN101762696A (en) | Chlamydia pneumoniae antibody assay kit and preparation method thereof | |
| CN105585633B (en) | The immune chromatography reagent kit of anti-human haemophilus influenzae P6 protein antibodies and the application antibody | |
| CN106932592A (en) | Detect colloidal gold strip of people's surfactant protein A and its preparation method and application | |
| JP4769171B2 (en) | Body fluid sample filtration separation apparatus and method | |
| CN102360012A (en) | Immunochromatography detection reagent strip for combined detection of toxoplasmagondii IgG antibodies and total antibodies, and preparation method thereof | |
| CN104515850B (en) | Device for quick testing and method | |
| CN202256347U (en) | Reagent strips for joint detection of toxoplasma immunoglobulin M (IgM) antibody and total antibody |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180427 Address after: 261023 No. 699, Yue He road, Weifang Economic Development Zone, Shandong Patentee after: WEIFANG KANGHUA BIOTECH Co.,Ltd. Address before: 261041 No. 699, Yue He road, Weifang Economic Development Zone, Shandong Patentee before: Yang Zhiting |
|
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: No.699, Yuehe Road, Weifang Economic Development Zone, Shandong Province Patentee after: Shandong Kanghua Biomedical Technology Co.,Ltd. Address before: 261023 Shandong city of Weifang Province River Road Economic Development Zone No. 699 Patentee before: WEIFANG KANGHUA BIOTECH Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130417 Termination date: 20220111 |