CN109085353A - A kind of Candida albicans colloidal-gold detecting-card and its application - Google Patents
A kind of Candida albicans colloidal-gold detecting-card and its application Download PDFInfo
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Abstract
The present invention relates to a kind of Candida albicans colloidal-gold detecting-cards, detection card includes test strips and detection card shell, test strips include colloidal gold film, nitrocellulose filter, sample pad, water absorption pad, PVC offset plate, sample pad, colloidal gold film, cellulose acetate film and water absorption pad are successively pasted on PVC offset plate, sample pad and colloidal gold film are side by side, cellulose acetate film one end slightly intersects with colloidal gold film, the other end slightly intersects with water absorption pad, test strips, which pass through, is cut into the strip slightly narrow with detection card width, the test strips of the strip are mounted in the flat shelly-shaped detection card shell of strip again, it detects card shell and is equipped with sample-adding window and testing result display window, sample pad is located at sample-adding window, colloidal gold film is located at testing result display window.The Candida albicans colloidal-gold detecting-card can be effectively used for measurement Candida albicans, and method is simple, convenient, fast, as a result accurately.
Description
Technical field
The present invention relates to candida albicans detection field, in particular to a kind of Candida albicans colloidal-gold detecting-card and its application.
Background technique
Candida albicans is typical conditioned pathogen, is carried on the internal of almost all people.With infection frequency height, face is infected
The features such as wide, especially in immunodeficiency type patient, organ transplant patients, diabetes patient, cancer patient, old man and baby
Disease incidence is relatively high.The Vulva vagina infection disease incidence of especially women is especially high, and almost everyone in life will face
Face more than once.
Currently, common detection method is vaginal discharge smear in hospital, then pass through optical microphotograph sem observation.Such method is detached from
Instrument readings lack the measurement standard of a quantization too much dependent on artificial judgement to define coccidioidomycosis.And it is some small
Type clinic, or even the family doctor of part America and Europe can be directly administered according to symptom, and misdiagnosis rate is higher.In daily life, because of disease
The reasons such as shape is not violent, and rhythm of life is fast, and difficult or even idea of seeing a doctor is conservative also often result in the ignorance to the disease and affect adversely and control
It treats.So establish it is a kind of simple, effectively, the measuring method of household be extremely necessary.
Summary of the invention
It is more suitable for patient the object of the present invention is to provide one kind and carries out self-test, and quick letter for Candida albicans
Just, low-cost qualitative detection card.
Technical scheme is as follows: a kind of Candida albicans colloidal-gold detecting-card, and the detection card includes test strips
And detection card shell, the test strips include colloidal gold film, nitrocellulose filter, sample pad, water absorption pad, PVC offset plate, described
Sample pad, colloidal gold film, cellulose acetate film and water absorption pad are successively pasted on PVC offset plate, and sample pad and colloidal gold film are simultaneously
Row, cellulose acetate film one end slightly intersect with colloidal gold film, and the other end slightly intersects with water absorption pad, and test strips are by being cut into and examining
The slightly narrow strip of card width is surveyed, then the test strips of the strip are mounted in the flat shelly-shaped detection card shell of strip, institute
It states detection card shell and is equipped with sample-adding window and testing result display window, sample pad is located at sample-adding window, the colloidal gold film
Positioned at testing result display window；The colloidal gold film has been coated with monoclonal antibody colloid gold label object；On nitrocellulose filter
The nature controlling line C for having the detection line T for being coated with Candida albicans surface antigen enolase Eno1 and being coated with sheep anti-mouse igg.
As one of preferred technical solution, the detection line T in the cellulose acetate film is located at a left side of nature controlling line C
Side, close to sample pad.
As one of preferred technical solution, the method for coating of the colloidal gold film are as follows:
(1) prepared by colloidal gold solution: taking 1L conical flask 1, ultrapure water 495mL is added, 1% gold chloride is then added
(HAuCl4·3H2O) 5mL is configured to 0.01% gold chloride (HAuCl of 500mL4·3H2O) aqueous solution, heating are being held after boiling
1% trisodium citrate (Na is added in the case where continuous stirring3C6H5O7·2H2O) solution 5-7mL continues agitating and heating, when solution
When color becomes transparent aubergine completely, stop heating after maintaining 5min, moisturizing to original volume is cooled to room temperature, 2-8 DEG C of guarantor
It deposits spare；
(2) pretreatment of antibody: the Eno1 antibody that will be marked is centrifuged 20min, takes under the conditions of 1000r/min, 4 DEG C
Supernatant is diluted to 1mg/mL with the PBS of 0.01mol/L, crosses 0.22 μm of filter membrane；
(3) preparation of colloid gold label object: taking the colloidal gold solution 40mL in step (1), with 0.25mol/L potassium carbonate
(K2CO3) colloidal gold solution pH to 8.5 is adjusted, 4mL antibody protein containing 0.3mg is added dropwise in magnetic stirrer 250r/min stirring
Solution reacts 10min；10% Bovine serum albumin of 4mL (BSA) is added dropwise, continues to be stirred to react 10min；Gold labeling antibody is molten
Liquid room temperature low speed (1500r/min) is centrifuged 10min, discards the precipitating formed by the gold particle agglomerated；4 DEG C of red supernatant solution,
12000r/min is centrifuged 20min, abandons supernatant, collects precipitating, precipitating is settled to 1mL with gold labeling antibody dilution, is prepared into
Eno1 monoclonal antibody colloid gold label object；
(4) preparation of colloidal gold film: by Eno1 monoclonal antibody colloid gold label object in step (3) with stroke film instrument with 5 μ l/
The even concentration of cm is sprayed on carrier glass cellulose membrane, and Dan Ke containing Eno1 is made in room temperature naturally dry or 37 DEG C of drying 3h
The colloidal gold film of grand antibody colloidal gold marker；
As one of preferred technical solution, the method for coating of the nitrocellulose filter are as follows: resist sheep anti-mouse igg
Body is diluted to 1mg/mL, is successively sprayed on nitrocellulose filter, is coated with into sheep anti-mouse igg with the concentration of 1 μ L/cm with film instrument is drawn
Antibody nature controlling line C；Eno1 antibody is diluted to 1mg/mL, nitrocellulose is successively sprayed on the concentration of 1 μ L/cm with film instrument is drawn
On film, it is coated with into Enol monoclonal antibody detection line T, then after 37 DEG C of coating 2h, room temperature naturally dry or 37 DEG C of drying.
As one of preferred technical solution, the sample pad the preparation method comprises the following steps: on glass fibre element film
After being coated with sample pad treatment fluid evenly, under conditions of air humidity is lower than 60%, room temperature naturally dry.
As one of preferred technical solution, the sample pad treatment fluid be by 1g BSA (Bovine serum albumin) and
0.8gNaCl (sodium chloride), with the 0.01mol/LPBS (phosphoric acid for containing 0.5%TRITON-100 (Triton X-100)
Salt buffer) it is settled to 100mL and forms.
The present invention also provides a kind of detection method of Candida albicans colloidal-gold detecting-card, the solids that needs are detected
The suspended matter sample detected after the sampling of sample object with the ultrapure water infiltration of 500 μ l or direct requirement draws 3-4 drop, drop with suction pipe
To window is loaded, read within 5 minutes as a result, then judging testing result, so i.e. completion according to the detection case of detection card
Pattern detection,
Test result judgement: display two lines, a detection line, a nature controlling line, then it represents that result is positive, and detects sample
There are Candida albicans in product；If only showing a line nature controlling line, then it represents that result is negative, and there is no whites in test sample
Candida albicans, if only showing a detection line, then it represents that testing result is invalid.
It is white in test sample that another object of the present invention is to provide a kind of Candida albicans colloidal-gold detecting-card
Application in candida albicans presence.
Compared with prior art, the invention has the benefit that Candida albicans colloidal gold provided by the present invention detects
Card has both high sensitivity and high specific, can quickly detect Candida albicans antigen；In addition, detection card have it is easy to operate,
The advantages that method is simple, rapid and simple, result is accurate, economic and practical.
Detailed description of the invention:
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention without any creative labor, may be used also for those of ordinary skill in the art
To obtain other drawings based on these drawings.
Fig. 1 is the structural schematic diagram of Candida albicans colloidal-gold detecting-card of the present invention；
Fig. 2 is the structural schematic diagram of test strips built in Candida albicans colloidal-gold detecting-card of the present invention；
Wherein, 1, sample-adding window；2, testing result display window；3, sample pad；4, colloidal gold film；5, PVC offset plate；6, vinegar
Acid cellulose film；7, nature controlling line C；8, detection line T；9, water absorption pad,
Hollow square indicates the Enol in test sample；Filled circles represent Jin Biao.
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification
Other advantages and efficacy of the present invention can be easily understood for disclosed content.
The nomenclature of drug table of comparisons is as follows in the present invention:
Embodiment 1
As depicted in figs. 1 and 2, a kind of Candida albicans colloidal-gold detecting-card, including test strips and detection card shell, institute
Stating test strips includes colloidal gold film 4, nitrocellulose filter 6, sample pad 3, water absorption pad 9, PVC offset plate 5, the sample pad 3, colloid
Golden film 4, cellulose acetate film 6 and water absorption pad 9 are successively pasted on PVC offset plate 5, sample pad 3 and colloidal gold film 4 side by side, vinegar
6 one end of acid cellulose film slightly intersects with colloidal gold film 4, and the other end slightly intersects with water absorption pad 9, and test strips are by being cut into and detecting
The slightly narrow strip of card width, then the test strips of strip are mounted in the flat shelly-shaped detection card shell of strip, the detection
Card shell is equipped with sample-adding window 1 and testing result display window 2.
It is as shown in Figure 2: the attached PVC offset plate of bottom in figure；A is sample pad；G is colloidal gold film, is coated in the colloidal gold film
Monoclonal antibody colloid gold label object；T, informal voucher where C is coated film, i.e. nitrocellulose filter, is wrapped on the nitrocellulose filter
By Eno1 monoclonal antibody and sheep anti-mouse igg；B is water absorption pad, is made of water-absorbent material such as filter paper.
(sample end) adheres to sample pad on one end of PVC offset plate A, is side by side configuration with colloidal gold film.
In PVC offset plate intermediate adhesion nitrocellulose filter, sheep anti-mouse igg nature controlling line C is provided on nitrocellulose filter
With Eno1 monoclonal antibody detection line T.
Water absorption pad is adhered at the end offset plate B PVC, one end of nitrocellulose filter slightly intersects with film, and the other end and water absorption pad B are omited
Intersect, which can be incorporated in detection card shell made of plastic mould, detection card be made, the A in detection card shell Fig. 1
Place is equipped with sample-adding window 1 and testing result display window 2, the sample pad face sample-adding window 1, nitrocellulose filter face Fig. 1
Middle testing result display window 2.
It detects the assembling of card: successively pasting sample pad, colloidal gold film, coated film and absorbent wool on PVC offset plate, be assembled into examination
Paper slip is cut into one fixed width strip, then test strips is mounted in the flat shelly-shaped detection card shell of strip.
Embodiment 2
The preparation method of Candida albicans colloidal-gold detecting-card is realized by following steps:
(1) prepared by colloidal gold solution: taking 1L conical flask 1, ultrapure water 495mL is added, 1% gold chloride is then added
(HAuCl43H2O) 5mL is configured to 500mL0.01% aqueous solution of chloraurate, heats after boiling in the case where lasting stirring
1% trisodium citrate (Na3C6H5O72H2O) solution 5-7mL is added, continues agitating and heating, when the color of solution becomes completely
When transparent aubergine, stop heating after maintaining 5min, moisturizing to original volume is cooled to room temperature, and 2-8 DEG C saves backup；
(2) pretreatment of antibody: the Eno1 antibody that will be marked is centrifuged 20min, takes under the conditions of 1000r/min, 4 DEG C
Supernatant is diluted to 1mg/mL with 0.01mol/LPBS, crosses 0.22 μm of filter membrane；
(3) preparation of colloid gold label object: taking the colloidal gold solution 40mL in step (1), with 0.25mol/LK2CO3 tune
Colloidal gold solution pH to 8.5 is saved, 4mL antibody protein solution containing 0.3mg is added dropwise, instead in magnetic stirrer 250r/min stirring
Answer 10min；4mL10% Bovine serum albumin (BSA) is added dropwise, continues to be stirred to react 10min；Gold labeling antibody solution room temperature is low
Fast (1500r/min) is centrifuged 10min, discards the precipitating formed by the gold particle agglomerated；4 DEG C of red supernatant solution, 12000r/
Min is centrifuged 20min, abandons supernatant, collects precipitating, precipitating is settled to 1mL with gold labeling antibody dilution, is prepared into Eno1 monoclonal
Antibody colloidal gold marker；
(4) preparation of colloidal gold film: by step (3) Eno1 monoclonal antibody colloid gold label object with stroke film instrument with 5 μ L/cm
Even concentration be sprayed on carrier glass cellulose membrane, monoclonal containing Eno1 is made in room temperature naturally dry or 37 DEG C of drying 3h
The colloidal gold film of antibody colloidal gold marker；
(5) it is coated with film preparation: sheep anti-mouse igg antibody, Eno1 antibody is diluted to 1mg/mL, with stroke film instrument successively with 1 μ L/
The concentration of cm is sprayed on nitrocellulose filter, is prepared into coated film, after 37 DEG C of coating 2h, room temperature naturally dry or 37 DEG C of bakings
It is dry；
(6) sample pad pre-treatment: sample pad treatment fluid is uniformly coated on glass fibre element film, is lower than in air humidity
Under conditions of 60%, room temperature naturally dry；
(7) assembling of detection card: sample pad, colloidal gold film, coated film and absorbent wool are successively pasted on PVC offset plate, is assembled
At test strips, it is cut into the strip of one fixed width, then test strips are mounted in the flat shelly-shaped detection card shell of strip.
In step (5) coated detection line T be located at the left (such as Fig. 1) of nature controlling line C；
Sample pad treatment fluid in step (6) is by 1g Bovine serum albumin (BSA) and 0.8g sodium chloride (NaCl), with containing
There is the 0.01mol/LPBS of 0.5%TRITON-100 to be settled to 100mL.
Embodiment 3
A kind of detection method of Candida albicans colloidal-gold detecting-card is used after needing the solids sample object detected sampling
The ultrapure water infiltration of 500 μ l or the suspended matter sample of direct requirement detection, draw 3-4 drop with suction pipe, drip to sample-adding window, and 5 points
It is read within clock as a result, then judge testing result according to the detection case of detection card, is in this way to complete pattern detection,
Test result judgement: display two lines, a detection line, a nature controlling line, then it represents that result is positive, and detects sample
There are Candida albicans in product；If only showing a line nature controlling line, then it represents that result is negative, and there is no whites in test sample
Candida albicans, if only showing a detection line, then it represents that testing result is invalid.
The above shows and describes the basic principle, main features and advantages of the invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (8)
1. a kind of Candida albicans colloidal-gold detecting-card, it is characterised in that: the detection card includes that test strips and detection card are outer
Shell, the test strips include colloidal gold film, nitrocellulose filter, sample pad, water absorption pad, PVC offset plate, the sample pad, colloid
Golden film, cellulose acetate film and water absorption pad are successively pasted on PVC offset plate, sample pad and colloidal gold film side by side, acetate fiber
Plain film one end slightly intersects with colloidal gold film, and the other end slightly intersects with water absorption pad, and test strips are omited by being cut into detection card width
Narrow strip, then the test strips of the strip are mounted in the flat shelly-shaped detection card shell of strip, the detection card is outer
Shell is equipped with sample-adding window and testing result display window, and sample pad is located at sample-adding window, and the colloidal gold film is located at detection knot
Fruit display window；The colloidal gold film has been coated with monoclonal antibody colloid gold label object；It has been coated on nitrocellulose filter white
The detection line T of the color candida albicans surface antigen enolase Eno1 and nature controlling line C for being coated with sheep anti-mouse igg.
2. a kind of Candida albicans colloidal-gold detecting-card according to claim 1, it is characterised in that: the cellulose acetate
Detection line T on film is located at the left of nature controlling line C, close to sample pad.
3. a kind of Candida albicans colloidal-gold detecting-card according to claim 1, it is characterised in that: the colloidal gold film
Method for coating are as follows:
(1) prepared by colloidal gold solution: taking 1L conical flask 1, ultrapure water 495mL is added, 1%HAuCl is then added4·3H2O
5mL is configured to 500mL 0.01%HAuCl4·3H2O aqueous solution, heating are added 1% in the case where lasting stirring after boiling
Na3C6H5O7·2H2O solution 5-7mL continues agitating and heating, when the color of solution becomes transparent aubergine completely, maintains
Stop heating after 5min, moisturizing to original volume is cooled to room temperature, and 2-8 DEG C saves backup；
(2) pretreatment of antibody: the Eno1 antibody that will be marked is centrifuged 20min, takes supernatant under the conditions of 1000r/min, 4 DEG C,
It is diluted to 1mg/mL with 0.01mol/L, the PBS of pH7.2-7.4, crosses 0.22 μm of filter membrane；
(3) preparation of colloid gold label object: the colloidal gold solution 40mL in step (1) is taken, 0.25mol/LK is used2CO3Adjust colloid
Gold solution pH to 8.5, magnetic stirrer 250r/min stirring, are added dropwise 4mL antibody protein solution containing 0.3mg, react
10min；4mL 10%BSA is added dropwise, continues to be stirred to react 10min；Gold labeling antibody solution room temperature is with the low speed of 1500r/min
It is centrifuged 10min, discards the precipitating formed by the gold particle agglomerated；4 DEG C of red supernatant solution, 12000r/min are centrifuged 20min, abandon
Supernatant collects precipitating, precipitating is settled to 1mL with gold labeling antibody dilution, is prepared into Eno1 monoclonal antibody colloid gold label
Object；
(4) preparation of colloidal gold film: by Eno1 monoclonal antibody colloid gold label object in step (3) with stroke film instrument with 5 μ l/cm
Even concentration be sprayed on carrier glass cellulose membrane, monoclonal containing Eno1 is made in room temperature naturally dry or 37 DEG C of drying 3h
The colloidal gold film of antibody colloidal gold marker.
4. a kind of Candida albicans colloidal-gold detecting-card according to claim 1, it is characterised in that: the nitrocellulose
The method for coating of film are as follows: sheep anti-mouse igg antibody is diluted to 1mg/mL, nitre is successively sprayed on the concentration of 1 μ L/cm with film instrument is drawn
On acid cellulose film, it is coated with into sheep anti-mouse igg antibody nature controlling line C；Eno1 antibody is diluted to 1mg/mL, with draw film instrument successively with
The concentration of 1 μ L/cm is sprayed on nitrocellulose filter, and coating is at Enol monoclonal antibody detection line T, then 37 DEG C of coating 2h
Afterwards, room temperature naturally dry or 37 DEG C of drying.
5. a kind of Candida albicans colloidal-gold detecting-card according to claim 1, it is characterised in that: the system of the sample pad
Preparation Method are as follows: after being equably coated with upper sample pad treatment fluid on glass fibre element film, be lower than 60% condition in air humidity
Under, room temperature naturally dry.
6. a kind of Candida albicans colloidal-gold detecting-card according to claim 5, it is characterised in that: the sample pad processing
Liquid is to be settled to 100mL by 1g BSA and 0.8g NaCl with the 0.01mol/LPBS containing 0.5%TRITON-100 and formed.
7. a kind of detection method of Candida albicans colloidal-gold detecting-card according to claim 1-6, feature
It is:
The suspended matter sample that will be detected after the solids sample object sampling for needing to detect with the ultrapure water infiltration of 500 μ l or direct requirement
Product, draw 3-4 drop with suction pipe, drip to sample-adding window, are read within 5 minutes as a result, then being sentenced according to the detection case of detection card
Disconnected testing result, completes pattern detection in this way；
Test result judgement: display two lines, a detection line, a nature controlling line, then it represents that there is white in test sample and read
Pearl bacterium；If only showing a line nature controlling line, then it represents that Candida albicans is not present in test sample, if only showing a detection
Line, then it represents that testing result is invalid.
8. a kind of application of Candida albicans colloidal-gold detecting-card in test sample in Candida albicans presence.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109613239A (en) * | 2018-12-27 | 2019-04-12 | 迈克生物股份有限公司 | A rotavirus Test paper |
| CN110244049A (en) * | 2019-07-31 | 2019-09-17 | 河南美凯生物科技有限公司 | Pathogenic Candida detection and identification method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002088741A1 (en) * | 2001-04-25 | 2002-11-07 | Rockeby Biomed Ltd | Detection of candida |
| CN1645145A (en) * | 2004-08-09 | 2005-07-27 | 中国人民解放军南京军区南京总医院 | Method and reagent box for inspecting mycelian protein antibody of white candida |
| CN103808930A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Zilpaterol immune colloidal gold detection card and preparation method thereof |
| CN104991060A (en) * | 2015-06-01 | 2015-10-21 | 上海凯创生物技术有限公司 | Canidia albicans antigen colloidal gold detection kit |
| CN105004865A (en) * | 2015-07-21 | 2015-10-28 | 贺政新 | Candida albicans enolase antibody test paper |
| CN206057335U (en) * | 2016-08-31 | 2017-03-29 | 长沙励思生物技术有限公司 | Chromatography and enzyme chemical method joint-detection gynecology colpitis test kit |
| CN107389940A (en) * | 2017-08-23 | 2017-11-24 | 潍坊市康华生物技术有限公司 | A kind of vaginitis multi-link detection reagent kit and preparation method thereof |
| CN207067145U (en) * | 2017-08-23 | 2018-03-02 | 潍坊市康华生物技术有限公司 | A kind of vaginitis multi-link detection reagent kit |
-
2018
- 2018-06-13 CN CN201810619649.XA patent/CN109085353A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002088741A1 (en) * | 2001-04-25 | 2002-11-07 | Rockeby Biomed Ltd | Detection of candida |
| CN1645145A (en) * | 2004-08-09 | 2005-07-27 | 中国人民解放军南京军区南京总医院 | Method and reagent box for inspecting mycelian protein antibody of white candida |
| CN103808930A (en) * | 2012-11-06 | 2014-05-21 | 江苏维赛科技生物发展有限公司 | Zilpaterol immune colloidal gold detection card and preparation method thereof |
| CN104991060A (en) * | 2015-06-01 | 2015-10-21 | 上海凯创生物技术有限公司 | Canidia albicans antigen colloidal gold detection kit |
| CN105004865A (en) * | 2015-07-21 | 2015-10-28 | 贺政新 | Candida albicans enolase antibody test paper |
| CN206057335U (en) * | 2016-08-31 | 2017-03-29 | 长沙励思生物技术有限公司 | Chromatography and enzyme chemical method joint-detection gynecology colpitis test kit |
| CN107389940A (en) * | 2017-08-23 | 2017-11-24 | 潍坊市康华生物技术有限公司 | A kind of vaginitis multi-link detection reagent kit and preparation method thereof |
| CN207067145U (en) * | 2017-08-23 | 2018-03-02 | 潍坊市康华生物技术有限公司 | A kind of vaginitis multi-link detection reagent kit |
Non-Patent Citations (1)
| Title |
|---|
| 胡毓安 等: "白念珠菌烯醇化酶双抗体夹心ELISA检测方法的建立", 《临床检验杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109613239A (en) * | 2018-12-27 | 2019-04-12 | 迈克生物股份有限公司 | A rotavirus Test paper |
| CN109613239B (en) * | 2018-12-27 | 2022-02-08 | 迈克生物股份有限公司 | Group A rotavirus detection test paper |
| CN110244049A (en) * | 2019-07-31 | 2019-09-17 | 河南美凯生物科技有限公司 | Pathogenic Candida detection and identification method |
| CN110244049B (en) * | 2019-07-31 | 2022-05-06 | 河南美凯生物科技有限公司 | Pathogenic candida detection and identification method |
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