CN206686950U - A kind of culture of nematodes cup and culture of nematodes device - Google Patents
A kind of culture of nematodes cup and culture of nematodes device Download PDFInfo
- Publication number
- CN206686950U CN206686950U CN201720194001.3U CN201720194001U CN206686950U CN 206686950 U CN206686950 U CN 206686950U CN 201720194001 U CN201720194001 U CN 201720194001U CN 206686950 U CN206686950 U CN 206686950U
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- cup
- culture
- nematodes
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Abstract
Culture apparatus field is the utility model is related to, in particular to a kind of culture of nematodes cup and culture of nematodes device.The utility model discloses a kind of culture of nematodes cup, including the first culture cup and the second culture cup.Second culture cup is contained in the first culture cup, and location division is provided with the rim of a cup of the second culture cup, and location division and the rim of a cup of the first culture cup coordinate, and water space is formed between the bottom of a cup of the second culture cup and the bottom of a cup of the first culture cup.The bottom of a cup of second culture cup is provided with multiple through holes, and film is provided with the rim of a cup of the second culture cup, and film is provided with multiple air-vents.The culture cup can ensure the humidity in the second culture cup by the water in water space, realize the culture of sample, and can the more convenient culture situation intuitively observed in culture cup, simplify the operation of culture, experimenter is greatly facilitated, saves the time.Based on above-mentioned culture of nematodes cup, the invention also discloses a kind of culture of nematodes device.
Description
Technical field
Culture apparatus field is the utility model is related to, in particular to a kind of culture of nematodes cup and culture of nematodes device.
Background technology
Because the symptom of the linear parasitic disease of domestic animal intestines and stomach is generally deficient of specificity, therefore, only it is difficult by clinical symptoms
Reliable diagnosis is made to Biocontrol of Gastrointestinal Nematodes in Livestock, is largely dependent upon the inspection in laboratory.With laboratory examination side
Method inspection is detected the contents such as the excrement of domestic animal, urine, finds worm's ovum, larva, polypide or polypide fragment, and according to the worm to being found
Correct diagnosis is made in the identification of ovum etc..
The worm's ovum of most gastrointestinal nematode parasites excretes with excrement, and it is suitable in vitro under the conditions of further develop
As larva.Typically by checking the worm's ovum among excrement, and carry out identification can obtain animal infection gastrointestinal parasite
Information, but some worm's ovum similarities are very high, and the nematode worm's ovum as belonging to Strongylida is difficult morphologically to distinguish.Therefore need by
Worm's ovum is hatched, and when ovulation development is larva, larva is identified.
Traditional cultural method need to keep having higher humidity in culture dish during larvae, it is therefore desirable to
The continual humidity checked in control culture dish in the training period, traditional training method is due to using the side manually observed
Method so that must into ware, spray water is to keep necessary humidity within multiple periods by someone daily, such embodiment is not
The input of excessive personnel is only caused, also add the cost of culture.
Secondly, such training method needs to collect the globule that ware covers after 7 days, and observe under the microscope just can be with
It is determined that so that it can not accomplish to check and observe in real time among incubation, seriously affect whole incubation experiment
The collection of data and the analysis of result.
Utility model content
The purpose of this utility model is to provide a kind of culture of nematodes cup, and the second culture is able to maintain that by the first culture cup
The humidity of cup, can effectively reduce the labor intensity of experimenter, while can realize to culture cup by the first culture cup
Interior culture of nematodes situation carries out observation and Data Collection in real time, facilitates follow-up experimental analysis.The culture of nematodes cup can
The accuracy of experimental result is improved while using manpower and material resources sparingly.
Another object of the present utility model is the provision of a kind of culture of nematodes device, and the culture of nematodes device passes through constant temperature
Incubator is able to maintain that the temperature in incubator, and keeps wet in the second culture cup by the moisture evaporation in water space
Degree, simplifies the operation of culture, greatly facilitates experimenter, save the time.
What embodiment of the present utility model was realized in：
A kind of culture of nematodes cup, including the first culture cup and the second culture cup, the second culture cup are contained in the first culture cup
It is interior, and location division is provided with the rim of a cup of the second culture cup, location division and the rim of a cup of the first culture cup coordinate.Second culture cup
Water space is formed between the bottom of a cup of bottom of a cup and the first culture cup, the bottom of a cup of the second culture cup is provided with multiple through holes.Second training
Support and be provided with film at the rim of a cup of cup, film is provided with multiple air-vents.
The culture of nematodes cup includes the first culture cup and the second culture cup, and the second culture cup is contained in the first culture cup
Inside, formed water space.It is provided among the process cultivated, at the rim of a cup of the second culture cup and is provided with the thin of air-vent
Film, while air permeable effect is realized, by the evaporation of the water in water space, enter by the through hole of the second culture cup bottom
Into the second culture cup, so as to maintain the humidity in the second culture cup needed for culture sample.In addition, in the second culture cup
Sample in the nematode turned out will also pass through the through hole of the second culture cup bottom of a cup and enter in water space so that cultivated
Larva separates with the sample cultivated, so that the observation of naked eyes is more convenient, spent manpower and materials are also more in the training period
Few, operation is simpler, and make it that the Data Collection among incubation is more accurate, is made according to collected data
Interpretation of result is also relatively reliable.
In a kind of embodiment of the present utility model：
Location division is the hangers to be formed that stretches out at the rim of a cup of the second culture cup.
In a kind of embodiment of the present utility model：
Sealing gasket is provided between the rim of a cup of location division and the first culture cup.
In a kind of embodiment of the present utility model：
Ventilative backing plate is installed in the bottom of a cup of the second culture cup.
In a kind of embodiment of the present utility model：
First culture cup and the second culture cup are made of clear material.
In a kind of embodiment of the present utility model：
Water level is provided with the wall of cup of first culture cup below the bottom of a cup of the second culture cup to indicate.
In a kind of embodiment of the present utility model：
The diameter of second culture cup is gradually reduced from rim of a cup to bottom of a cup direction.
In a kind of embodiment of the present utility model：
The bottom of a cup of second culture cup is raised to rim of a cup direction.
In a kind of embodiment of the present utility model：
Second culture cup is also equipped with the cup lid coordinated with the rim of a cup of the second culture cup, and cup lid is provided with air-vent.
A kind of culture of nematodes device, including constant incubator and the above-mentioned culture of nematodes that is positioned in constant incubator
Cup.
The culture of nematodes device employs above-mentioned culture of nematodes cup, passes through constant incubator culture of nematodes cup interior energy
The enough humidity maintained needed for culture nematode, reduces required manpower and materials, operation is simpler, observes convenience more so that
Data Collection among incubation is more accurate.
The technical solution of the utility model at least possesses following beneficial effect：
Culture of nematodes cup provided by the utility model, can reduce needed for manpower and materials while, simplified culture
Operation so that the convenience observed more is direct, improves the degree of accuracy of Data Collection, the result point made according to collected data
Analysis is also relatively reliable.
Culture of nematodes device provided by the utility model, the culture of nematodes device employ above-mentioned culture of nematodes cup, lead to
The temperature crossed in constant incubator maintenance incubator, and keep wet in the second culture cup by the moisture in water space
Degree, simplifies the operation of culture, greatly facilitates experimenter, save the time.
Brief description of the drawings
, below will be to required use in embodiment in order to illustrate more clearly of the technical scheme of the utility model embodiment
Accompanying drawing be briefly described, it will be appreciated that the following drawings illustrate only some embodiments of the present utility model, therefore should not be by
Regard the restriction to scope as, for those of ordinary skill in the art, on the premise of not paying creative work, may be used also
To obtain other related accompanying drawings according to these accompanying drawings.
Fig. 1 is the structural representation of nematode culture cup in the utility model embodiment 1；
Fig. 2 is the structural representation of the second culture cup in the utility model embodiment 1；
Fig. 3 is the enlarged drawing at IV in Fig. 2；
Fig. 4 is the enlarged drawing at V in Fig. 1.
Icon：200- culture of nematodes cups；The culture cups of 210- first；The culture cups of 220- second；230- films；221- is positioned
Portion；222- through holes；231- air-vents.
Embodiment
It is new below in conjunction with this practicality to make the purpose, technical scheme and advantage of the utility model embodiment clearer
Accompanying drawing in type embodiment, the technical scheme in the embodiment of the utility model is clearly and completely described, it is clear that is retouched
The embodiment stated is the utility model part of the embodiment, rather than whole embodiments.Generally here described in accompanying drawing and
The component of the utility model embodiment shown can be configured to arrange and design with a variety of.
Therefore, the detailed description of the embodiment of the present utility model to providing in the accompanying drawings is not intended to limit requirement below
The scope of the utility model of protection, but it is merely representative of selected embodiment of the present utility model.Based in the utility model
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of the utility model protection.
It should be noted that：Similar label and letter represents similar terms in following accompanying drawing, therefore, once a certain Xiang Yi
It is defined, then it further need not be defined and explained in subsequent accompanying drawing in individual accompanying drawing.
In description of the present utility model, it is necessary to explanation, term " interior ", " under " etc. instruction orientation or position relationship
For based on orientation shown in the drawings or position relationship, or the utility model product using when the orientation or position usually put
Relation, it is for only for ease of description the utility model and simplifies description, rather than indicates or imply that signified device or element must
There must be specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to limitation of the present utility model.This
Outside, term " first ", " second " etc. are only used for distinguishing description, and it is not intended that instruction or hint relative importance.
Embodiment 1
Fig. 1 is refer to, Fig. 1 shows the primary structure of the culture of nematodes cup 200, from figure 1 it appears that culture of nematodes
Cup 200 mainly includes the first culture cup 210 and the second culture cup 220.And the second culture cup 220 is contained in the first culture cup
In 210.
From figure 2 it can be seen that be provided with location division 221 at the rim of a cup of the second culture cup 220, incorporated by reference to Fig. 1 as can be seen that
Coordinated by the rim of a cup of the culture cup 210 of location division 221 and first of the second culture cup 220 so that the second culture cup 220 is positioned over
In first culture cup 210.
Referring once again to Fig. 1, in the inner space of the first culture cup 210, the bottom of a cup of the second culture cup 220 and the first training
Water space is formed between the bottom of a cup of foster cup 210, the water space is used to store water.
Fig. 3 is refer to, and can be seen that with reference to Fig. 1 and multiple through holes 222 are provided with the bottom of a cup of the second culture cup 220, is led to
The multiple through holes 222 crossed at the bottom of a cup of the second culture cup 220 can turn in the second culture cup 220 and water space so that position
It can be entered in the steam of water space by through hole 222 in the second culture cup 220.
Fig. 4 is refer to, film 230 is additionally provided with the rim of a cup of the second culture cup 220, and film 230 is provided with multiple
Air-vent 231, the air that the air-vent 231 is used to ensure in nematode culture cup 200 can be with the air circulations in the external world.
In summary, the culture of nematodes cup 200 includes the first culture cup 210 and the second culture cup 220, and the second culture
Cup 220 is contained in the inside of the first culture cup 210, forms water space.Among the process cultivated, the second culture cup
The film 230 for being provided with air-vent 231 is provided with 220 rim of a cup, while air permeable effect is realized, passes through the water in water space
Evaporation, entered by the through hole 222 of the bottom of the second culture cup 220 in the second culture cup 220, so as to maintain the second training
Support the humidity needed for culture sample in cup 220.In addition, the nematode turned out in sample in the second culture cup 220 also can be through
The through hole 222 for crossing the bottom of a cup of the second culture cup 220 is entered in water space so that the larva cultivated and the sample point of culture
From, so that the observation of naked eyes is more convenient, spent manpower and materials are also less in the training period, and operation is simpler, and
And make it that the Data Collection among incubation is more accurate, the interpretation of result made according to collected data also more may be used
Lean on.
Below by realizing that the concrete structure of the effect above is described：
First, when the effect of location division 221 is to enable the second culture cup 220 be contained in the first culture cup 210
Keep a relative position, reference picture 1 finds out, due among the process of culture, it is necessary to ensure water in water space with
Second culture cup 220 keeps a relative distance, avoids the bottom of a cup of the second culture cup 220 from being immersed in water space and has influence on
Sample at the bottom of a cup of the second culture cup 220, therefore the relative card of rim of a cup for passing through the culture cup 220 of location division 221 second
At the rim of a cup of the first culture cup 210, certain protection and position-limiting action are played.Therefore in the present embodiment, location division 221 is preferred
For the hangers to be formed that stretches out at the rim of a cup of the second culture cup 220.Stretched out the hangers to be formed by this, can be effective
The second culture cup of limitation 220 be contained in when in the first culture cup 210 along moving in short transverse, prevent the second culture cup
220 bottom of a cup is immersed in water space, has influence on the culture of nematode.
Secondly, among the process for install the second culture cup 220, in order that obtaining the caused steam in water space
The through hole 222 at the bottom of a cup by the second culture cup 220 is merely able to enter in the second culture cup 220, therefore in location division 221
Sealing gasket is provided between the rim of a cup of the first culture cup 210.By the cup that the passage of steam is limited to the second culture cup 220
The through hole 222 of bottom so that steam can only be discharged into by through hole 222 in second culture cup 220 or via through holes 222 enter nematode
Outside culture cup 200, it ensure that caused air to the humidifying effect of the second culture cup 220, is improved and trained in water space
Support the stability among the process of nematode.
Among the process cultivated, common cultural method is by by the sample gauze wrapped of culture and placing
At the bottom of a cup of the second culture cup 220, such method can cause the process of preparation more, it is necessary to expend the regular hour.
In other embodiment of the present utility model, ventilative backing plate is installed in the bottom of a cup of the second culture cup 220.By the way that culture sample is straight
Connect that be placed on ventilative backing plate can be on the premise of culture effect not be influenceed, the process that reduces culture, reduce needed for preparation
Time.It is additionally, since and additional ventilative backing plate is provided with bottom of a cup, therefore chi can be opened up at the bottom of a cup of the second culture cup 220
Very little larger through hole 222, increases ventilative effect, can preferably maintain the humidity in the second culture cup 220.Meanwhile also can
Enough nematodes for more easily making to turn out in the second culture cup 220 are entered in water space so that the effect energy of culture
Enough more accurate.
In the present embodiment, in order in the water filling into water space, it was observed that the situation of water level, prevents water level mistake
It is high；When observing the sample culture situation of larva quantity and observation in water space in the second culture cup 220, Neng Gougeng
Directly observed to be simple, therefore the first culture cup 210 and the second culture cup 220 are made of clear material.Through the transparent of cup
Material can observe directly the inner case of the first culture cup 210 and the second culture cup 220.
Because the water level in water space needs to be maintained at the second culture cup 220 being positioned in the first culture cup 210
Below bottom, into water space among the process of water filling, when the second culture cup 220 is not positioned in the first culture cup 210
When, water level can not accurately be controlled by only observing water level by transparent cup, therefore in other embodiment of the present utility model
In, it is provided with water level below the bottom of a cup of the second culture cup 220 on the wall of cup of the first culture cup 210 and indicates.Water level sign is set
It is placed on the wall of cup of the first culture cup 210, and close to the bottom of a cup for the second culture cup 220 being contained in the first culture cup 210
Lower section, when thus even water level is added to water level sign, its water level does not interfere with the work of culture yet, as long as ensureing that water storage is empty
Between water level be no more than water level sign and just disclosure satisfy that the requirement of culture.
In the present embodiment, installed and taken relative to the first culture cup 210 in order that obtaining the second culture cup 220
Can more it facilitate among the process gone out and simple, therefore the diameter of the second culture cup 220 is gradually reduced from rim of a cup to bottom of a cup direction.
In other embodiment of the present utility model, in can also being arranged at bottom of a cup to cup for the second culture cup 220
Mouth direction is raised.By the way that bottom of a cup is arranged into raised shape, the receiving area of bottom of a cup, increase and sky below bottom of a cup can be increased
Between contact so that can improve the effect of culture more fully with moisture in the culture sample of the second culture cup 220
Fruit.
It should be noted that it is used for ventilative film 230 covered with one layer at the rim of a cup of the second culture cup 220, at this
In the other embodiment of utility model, because the culture cup belongs to the culture apparatus that can be recycled for multiple times, therefore second cultivates
Cup 220 is also equipped with the cup lid coordinated with the rim of a cup of the second culture cup 220, and cup lid is provided with air-vent 231.By set one can
The cup lid of dismounting, enable to save material among the process of culture, avoid the waste of resource, reduce the cost of experiment.
The operation principle of the culture of nematodes cup 200 is：
By the way that the second culture cup 220 to be contained in the inside of the first culture cup 210, and then form and pass through the second culture cup
220 bottom of a cup limits water space.
The sample of culture is positioned in the bottom of a cup of the second culture cup 220, among the process cultivated, the second culture
The film 230 for being provided with air-vent 231 is provided with the rim of a cup of cup 220, while air permeable effect is realized, by water space
The evaporation of water, entered by the through hole 222 of the bottom of the second culture cup 220 in the second culture cup 220, so as to maintain second
Humidity in culture cup 220 needed for culture sample.In addition, the nematode turned out in sample in the second culture cup 220 also can
Through hole 222 by the bottom of a cup of the second culture cup 220 is entered in water space so that the larva cultivated and the sample point of culture
From, so that the observation of naked eyes is more convenient, spent manpower and materials are also less in the training period, and operation is simpler, and
And make it that the Data Collection among incubation is more accurate, the interpretation of result made according to collected data also more may be used
Lean on.
It should be noted that：Using above-mentioned culture of nematodes cup 200, the invention also discloses a kind of culture of nematodes dress
Put.
The culture of nematodes device includes constant incubator and the culture of nematodes cup 200 being positioned in constant incubator.
The culture of nematodes device employs above-mentioned culture of nematodes cup 200, and is able to maintain that culture by constant incubator
Temperature in case, and the humidity in the second culture cup 220 is kept by the moisture in water space, simplify the behaviour of culture
Make, greatly facilitate experimenter, save the time.
Preferred embodiment of the present utility model is these are only, is not limited to the utility model, for this area
Technical staff for, the utility model can have various modifications and variations.It is all within the spirit and principles of the utility model,
Any modification, equivalent substitution and improvements made etc., should be included within the scope of protection of the utility model.
Claims (10)
- A kind of 1. culture of nematodes cup, it is characterised in that：The culture of nematodes cup includes the first culture cup and the second culture cup；Second culture cup is contained in first culture cup, and positioning is provided with the rim of a cup of second culture cup Portion, the location division and the rim of a cup of first culture cup coordinate；Water space is formed between the bottom of a cup of the bottom of a cup of second culture cup and first culture cup；The bottom of a cup of second culture cup is provided with multiple through holes；Film is provided with the rim of a cup of second culture cup, the film is provided with multiple air-vents.
- 2. culture of nematodes cup according to claim 1, it is characterised in that：The location division is in second culture cup Stretch out the hangers to be formed at rim of a cup.
- 3. culture of nematodes cup according to claim 1, it is characterised in that：In the location division and first culture cup Sealing gasket is provided between rim of a cup.
- 4. culture of nematodes cup according to claim 1, it is characterised in that：It is provided with the bottom of a cup of second culture cup Air cushion plate.
- 5. culture of nematodes cup according to claim 1, it is characterised in that：First culture cup and second culture cup It is made of clear material.
- 6. culture of nematodes cup according to claim 1, it is characterised in that：Close to described on the wall of cup of first culture cup Water level is provided with below the bottom of a cup of second culture cup to indicate.
- 7. culture of nematodes cup according to claim 1, it is characterised in that：The diameter of second culture cup is from rim of a cup to cup Bottom direction is gradually reduced.
- 8. culture of nematodes cup according to claim 1, it is characterised in that：The bottom of a cup of second culture cup is to rim of a cup direction It is raised.
- 9. culture of nematodes cup according to claim 1, it is characterised in that：Second culture cup is also equipped with and described second The cup lid that the rim of a cup of culture cup coordinates, the cup lid are provided with air-vent.
- A kind of 10. culture of nematodes device, it is characterised in that：The culture of nematodes device includes constant incubator and is positioned over institute State the culture of nematodes cup as described in any one in claim 1-9 items in constant incubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720194001.3U CN206686950U (en) | 2017-03-01 | 2017-03-01 | A kind of culture of nematodes cup and culture of nematodes device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720194001.3U CN206686950U (en) | 2017-03-01 | 2017-03-01 | A kind of culture of nematodes cup and culture of nematodes device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206686950U true CN206686950U (en) | 2017-12-01 |
Family
ID=60439431
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720194001.3U Expired - Fee Related CN206686950U (en) | 2017-03-01 | 2017-03-01 | A kind of culture of nematodes cup and culture of nematodes device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206686950U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642465A (en) * | 2020-06-02 | 2020-09-11 | 石河子大学 | Breeding method of Altai hepialus larvae |
-
2017
- 2017-03-01 CN CN201720194001.3U patent/CN206686950U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642465A (en) * | 2020-06-02 | 2020-09-11 | 石河子大学 | Breeding method of Altai hepialus larvae |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171201 Termination date: 20200301 |