CN206459939U - Detect the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase - Google Patents
Detect the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase Download PDFInfo
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- CN206459939U CN206459939U CN201720160468.6U CN201720160468U CN206459939U CN 206459939 U CN206459939 U CN 206459939U CN 201720160468 U CN201720160468 U CN 201720160468U CN 206459939 U CN206459939 U CN 206459939U
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- China
- Prior art keywords
- alkaline phosphatase
- polymorphonuclear leukocytes
- cell
- cell instrument
- test tube
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Abstract
The utility model detects the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase, including liquid fluid system, optical system and signal acquiring system, and liquid fluid system includes upper flow pond, and bottom is flow chamber；The flow cell is provided with generating laser, and the right side of generating laser is provided with light barrier, and the right side of light barrier is provided with diode；Test tube spray post and test tube sensor are provided with the top of the flow chamber, test tube spray post has sample and enters pipe, and the lower section that sample enters pipe attracts arm provided with waste liquid, and sample enters pipe rear and is provided with test tube guiding device；The utility model detects that Polymorphonuclear Leukocytes Membrane alkaline phosphatase result is accurate, it was therefore concluded that true using simple, effectively assesses the antibody number that each cell is combined（AB/c）, indirect reaction cell mNAP to be measured expression quantity overcomes the defect of NAP traditional detections, and Preliminary Clinical result shows that it shows good application prospect in antidiastole of the bacterium with virus infection.
Description
Technical field
The utility model belongs to detection device technical field, more particularly to detects the thin of Polymorphonuclear Leukocytes Membrane alkaline phosphatase
Born of the same parents' instrument.
Background technology
Alkaline phosphatase is a kind of catalysis phosplate hydrolase, is distributed widely in each internal organs organ of human body, wherein with
Liver at most, is secondly kidney, bone, the tissue such as intestines and placenta.At least 4 kinds isodynamic enzymes of ALP, neutrophil leucocyte alkaline phosphatase
Enzyme belongs to liver bone kidney type ALP.NAP is one of mark enzyme of ripe neutrophil leucocyte.Although its biological function is still unclear so far
Chu, but detection NAP can evaluate functions of neutrophils.Clinic is usually used in bacterium infection and the antidiastole of virus infection,
At present, detection mNAP methods mainly have cytochemical staining and chemical light substrate method both at home and abroad.Cytochemical staining method operation step
Rapid cumbersome, time-consuming, result is easily influenceed by subjective factor, needs certain experience, repeatability poor, and is difficult to standardize；Hair
Light method determines NAP activity, and neutrophil leucocyte need to be isolated and purified by significantly improving accuracy, but determining preceding sample, and operating process is complicated,
The factor that is affected is more, it is difficult to meet routine clinical application.
Utility model content
In order to assess the antibody number that each cell is combined（AB/c）, indirect reaction cell mNAP to be measured expression quantity, foundation
Drain cell art detects Polymorphonuclear Leukocytes Membrane alkaline phosphatase expression of enzymes, reaches the purpose of quantitative detection, proposes that a kind of detection is neutral
The cell instrument of granulocyte film alkaline phosphatase.
The technical solution adopted in the utility model is as follows：
Detect the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase, including liquid fluid system, optical system and signal acquisition
System, liquid fluid system includes upper flow pond, and bottom is flow chamber；The flow cell is provided with generating laser, Laser emission
The right side of device is provided with light barrier, and the right side of light barrier is provided with diode；Test tube spray post and test tube are provided with the top of the flow chamber
Sensor, test tube spray post has sample and enters pipe, and the lower section that sample enters pipe attracts arm provided with waste liquid, and sample enters after pipe
Side is provided with test tube guiding device.
Further, the sample includes sample introduction needle into pipe, and drop retains system.
Further, the sample introduction needle is externally provided with sleeve.
Further, the generating laser is correspondingly provided with detector.
Further, cell instrument is provided with drawer, inside there is sheath fluid cylinder, waste liquid cylinder and sheath liquid filter.
Further, the sheath liquid filter is located between sheath fluid cylinder and waste liquid cylinder.
Further, sheath liquid filter upper end is provided with air adjustment pipeline and sheath fluid effuser, and sheath liquid filter has
Pedestal, pedestal connection sheath fluid flows into pipe.
Further, the waste liquid attracts arm to be provided with waste liquid attractor armed lever.
Further, O-ring is provided between liquid filter and pedestal.
The utility model has the advantages that：
The utility model is detected that Polymorphonuclear Leukocytes Membrane alkaline phosphatase result is accurate, it was therefore concluded that true, had using simple
Effect assesses the antibody number that each cell is combined（AB/c）, indirect reaction cell mNAP to be measured expression quantity, overcome NAP tradition inspection
The defect of survey, Preliminary Clinical result show its bacterium with virus infection antidiastole in show good application before
Scape.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the utility model sheath fluid filter structure schematic representation.
Embodiment
The utility model is described further below in conjunction with the accompanying drawings：
As illustrated in fig. 1 and 2, the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase, including liquid fluid system, optical system are detected
And signal acquiring system, it is characterised in that：Liquid fluid system includes upper flow pond, and bottom is flow chamber；
The flow cell 7 is provided with generating laser 9, and the right side of generating laser is provided with light barrier 10, the right side of light barrier
Side is provided with diode 11；
Test tube spray post 1 and test tube sensor 2 are provided with the top of the flow chamber, test tube spray post has sample and enters pipe
3, the lower section that sample enters pipe attracts arm 6 provided with waste liquid, and sample enters pipe rear and is provided with test tube guiding device 4.The sample enters
Pipe includes sample introduction needle, and drop retains system.The sample introduction needle is externally provided with sleeve.The generating laser is correspondingly provided with detection
Device.Cell instrument is provided with drawer, inside there is sheath fluid cylinder, waste liquid cylinder and sheath liquid filter.The sheath liquid filter is located at sheath fluid cylinder and useless
Between fluid cylinder.Sheath liquid filter upper end is provided with air adjustment pipeline 12 and sheath fluid effuser 14, and sheath liquid filter has pedestal
15, pedestal connection sheath fluid flows into pipe 16.The waste liquid attracts arm to be provided with waste liquid attractor armed lever 5.Liquid filter and pedestal it
Between be provided with O-ring 17.
Embodiment：
1. open cell instrument power supply.
2. opening other peripheries matches somebody with somebody stand-by power source, such as printer and MO machines.
3. open computer.
4. confirming that sheath stream fluid cylinder there are eight points of full FACS Flow, screw really (sheath fluid cylinder capacity is 4L).
5. waste liquid is outwelled, and add in waste liquid cylinder 200 ml domestic bleaching waters (waste liquid cylinder capacity is 4L).
6. pressure-reducing valve direction is adjusted in pressurization（Pressurize）Position.
7. exclude fluid flow tube road and the bubble in sheath liquid filter.
8. removing sample cell, PRIME functions are performed twice.
9. use 1ml PBS, HIGH RUN two minutes.
10. it can start to analyze sample.
Based on above-mentioned application method, Lianyungang First People's Hospital directed toward bacteria infected patient, to wherein blood culture sun
Property 30 patients detect serum C-reactive protein and Procalcitonin simultaneously.
Select the phycoerythrin anti-human ALP antibody of mark category and isotype control Ab, hemolysin, PE reagents.
Detection mNAP is carried out using the utility model：PE is applied to the antibody that each cell of hybridoma supematant assesse is combined
Number, based on PE and anti-ALP antibody 1：1 ratio sets up 1 standard curve to calculate the antibody number combined on each detection cell.Between
It is reversed to reflect the expression quantity for treating mNAP on cell.
And then detected and analyzed by the utility model, draw evaluation or comment on：
Influence of the different anti-coagulants to testing result：EDTA-K is respectively adopted with portion blood preparation2, sodium citrate, liver
Plain sodium observes its influence to testing result as anti-coagulants.
Saving specimen different temperatures, time influence on testing result：By same blood samples of patients sample preserve respectively indoors,
4 DEG C, the influence of observation temperature, time to testing result.
Precision Experiment：The blood preparation for taking 2 parts of mNAP contents different, replication 10 times, calculates crowd interior CV respectively
（%）；It is another to take 2 parts of mNAP contents different blood preparation point, 5 batches of progress replications, 2 every crowd, every crowd of time interval 1h, meter
Calculate CV between criticizing（%）.
It is described above, embodiment only of the present utility model, but the protection domain of utility model is not limited to
This, any those skilled in the art the change that can readily occur in or replace in the technical scope that the utility model is disclosed
Change, should all cover within the protection domain of utility model.
Claims (9)
1. the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase is detected, including liquid fluid system, optical system and signal acquisition system
System, it is characterised in that：Liquid fluid system includes upper flow pond, and bottom is flow chamber；
The flow cell（7）It is provided with generating laser（9）, the right side of generating laser is provided with light barrier（10）, light barrier
Right side is provided with diode（11）；
Test tube spray post is provided with the top of the flow chamber（1）With test tube sensor（2）, test tube spray post have sample enter pipe
（3）, the lower section that sample enters pipe attracts arm provided with waste liquid（6）, sample is into pipe rear provided with test tube guiding device（4）.
2. the cell instrument of detection Polymorphonuclear Leukocytes Membrane alkaline phosphatase according to claim 1, it is characterised in that：The sample
This entrance pipe includes sample introduction needle, and drop retains system.
3. the cell instrument of detection Polymorphonuclear Leukocytes Membrane alkaline phosphatase according to claim 2, it is characterised in that：It is described enter
Sample pin is externally provided with sleeve.
4. the cell instrument of detection Polymorphonuclear Leukocytes Membrane alkaline phosphatase according to claim 1, it is characterised in that：It is described to swash
Optical transmitting set is correspondingly provided with detector.
5. the cell instrument of detection Polymorphonuclear Leukocytes Membrane alkaline phosphatase according to claim 1, it is characterised in that：Cell instrument
Provided with drawer, inside there are sheath fluid cylinder, waste liquid cylinder and sheath liquid filter.
6. the cell instrument of detection Polymorphonuclear Leukocytes Membrane alkaline phosphatase according to claim 5, it is characterised in that：The sheath
Liquid filter is located between sheath fluid cylinder and waste liquid cylinder.
7. the cell instrument of detection Polymorphonuclear Leukocytes Membrane alkaline phosphatase according to claim 1, it is characterised in that：Sheath fluid mistake
Filter upper end is provided with air adjustment pipeline（12）With sheath fluid effuser（14）, sheath liquid filter has pedestal（15）, pedestal company
Connect sheath fluid and flow into pipe（16）.
8. the cell instrument of detection Polymorphonuclear Leukocytes Membrane alkaline phosphatase according to claim 1, it is characterised in that：It is described useless
Liquid attracts arm to be provided with waste liquid attractor armed lever（5）.
9. the cell instrument of detection Polymorphonuclear Leukocytes Membrane alkaline phosphatase according to claim 7, it is characterised in that：Liquid is filtered
O-ring is provided between device and pedestal（17）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720160468.6U CN206459939U (en) | 2017-02-22 | 2017-02-22 | Detect the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720160468.6U CN206459939U (en) | 2017-02-22 | 2017-02-22 | Detect the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206459939U true CN206459939U (en) | 2017-09-01 |
Family
ID=59690629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720160468.6U Expired - Fee Related CN206459939U (en) | 2017-02-22 | 2017-02-22 | Detect the cell instrument of Polymorphonuclear Leukocytes Membrane alkaline phosphatase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206459939U (en) |
-
2017
- 2017-02-22 CN CN201720160468.6U patent/CN206459939U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170901 Termination date: 20210222 |
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| CF01 | Termination of patent right due to non-payment of annual fee |