CN206396201U - A kind of collection device of cell culture fluid - Google Patents

Abstract

The utility model discloses a collection device for cell culture fluid, which comprises a cell fluid storage bottle, a support plate is installed inside the cell fluid storage bottle, and a clamp is fixedly installed on the outside of the pressure plate, and the clamp is placed through the The outer wall of the seat, the chuck runs through the installation opening, and the bottom end of the catheter is fixedly equipped with a liquid suction head. The collection device for cell culture fluid can take out the first connecting tube and the second connecting tube from the cell liquid storage bottle by pulling up the first connecting tube and the second connecting tube, and can take off the bottle cap by rotating the bottle cap , the chuck is pulled out from the installation port on the outer wall of the vertical plate, the fixed plate drives the bottom mounting cap away from the support rod, and the fixed plate is taken out of the cell solution and placed inside the bottle, the filter can be cleaned and replaced to avoid long-term use This makes the filtering effect of the filter screen poor, ensuring that the quality of the filtered cell fluid is better.

Description

A device for collecting cell culture fluid

technical field

The utility model relates to the technical field of cell culture test equipment structure, in particular to a collection device for cell culture fluid.

Background technique

In the cell culture of biomedical and chemical experiments, a large number of cells need to be cultured and expanded. A large amount of cell culture liquid will be produced in the cell expansion project. These culture liquids are either collected for harmless treatment, or recycled and reused. The aseptic conditions of cell culture operations and the cleanliness of the surrounding area are relatively high. A little carelessness in the operation will contaminate the cells, affect the normal operation of the experimenters, and cause the experiment to fail. However, there are still problems in the existing collection device for cell culture fluid. For example, the patent application number 201520765396.9 includes a cell fluid container, a pipette tip connected to the cell fluid container, and a vacuum that generates negative pressure in the cell fluid container. device, the upper mouth of the cell solution container is equipped with a sealing plug, and the cell liquid container and the pipette head are connected through the first hose through the sealing plug, and the hose is equipped with an operating handle to facilitate the manipulation of the pipette head to absorb cells. Liquid, a one-way valve is installed in the operating handle to control the flow of the cell liquid, the switch of the one-way valve is set on the operating handle, the vacuum device and the cell liquid container are connected through the second hose through the sealing plug, Although the substances in the cell fluid can be filtered and recovered, the filter screen cannot be cleaned and replaced. After a long period of use, the filter screen has a poor filtering effect, resulting in low quality of the filtered cell fluid.

Utility model content

The purpose of this utility model is to provide a collection device for cell culture fluid, so as to solve the problems raised in the above-mentioned background technology.

In order to achieve the above purpose, the utility model provides the following technical solutions: a collection device for cell culture fluid, including a cell fluid storage bottle, a support plate is installed inside the cell fluid storage bottle, and the left and right sides of the top of the support plate are Both are fixedly installed with vertical plates, the outer wall of the vertical plate is provided with a mounting opening, the outer side of the vertical plate is installed with a cross bar, the outer side of the horizontal bar is fixedly installed with a fixing seat, the left and right sides of the top of the support plate All are fixedly installed with a stable seat, the top of the stable seat is fixed with a support rod, the top of the support rod is fixed with a mounting cap, the top of the mounting cap is fixed with a fixed plate, and the inside of the fixed plate is fixed A filter screen is installed, the left and right sides of the fixed plate are fixedly installed with a placement seat, the inside of the placement seat is installed with a telescopic spring, the outer side of the telescopic spring is fixedly installed with a pressing plate, and the outer side of the described pressing plate is fixedly installed with a A chuck, the chuck runs through the outer wall of the placement seat, the chuck runs through the installation port, the top of the cell solution bottle is equipped with a bottle cap, and the left and right sides of the top of the bottle cap are fixedly installed with stabilizing sleeves , the left and right sides of the inner top of the cell liquid storage bottle are respectively equipped with a second connecting pipe and a first connecting pipe, and both the second connecting pipe and the first connecting pipe run through the outer wall of the cell liquid storage bottle and the bottle cap, so The bottom end of the second connecting pipe is fixedly equipped with a vacuum device, the bottom end of the first connecting pipe is fixedly installed with a liquid accumulation cylinder, the front outer wall of the described liquid accumulation cylinder is equipped with a valve, and the bottom end of the valve is fixedly installed There is a catheter, and the bottom end of the catheter is fixedly equipped with a liquid suction head.

Preferably, the outer walls of the second connecting pipe and the first connecting pipe are sleeved with collars, and a fixing block is fixedly installed on the outside of the collars.

Preferably, a fixing ring is fixedly installed on the outer wall of the right side of the liquid collection cylinder, and a hook is fixedly installed on the top right side of the cell solution storage bottle, and the fixing ring is matched with the hook.

Preferably, a sealing sleeve is fixedly installed at the connection between the second connecting pipe, the first connecting pipe and the cell solution storage bottle.

Preferably, a handle is fixedly installed on the left outer wall of the liquid accumulation cylinder.

Compared with the prior art, the beneficial effect of the utility model is: the collection device of the cell culture fluid, by pulling the first connecting tube and the second connecting tube upward, the first connecting tube and the second connecting tube can be separated from the cell liquid Put it in the bottle and take it out, rotate the bottle cap to remove the bottle cap, push the chuck inward, the chuck pushes the pressure plate to move inward, the pressure plate presses the telescopic spring to expand and contract, and the chuck pulls from the installation port on the outer wall of the vertical plate out, pull the fixing plate upwards, the fixing plate drives the mounting cap at the bottom away from the support rod, take the fixing plate out of the cell solution and place it inside the bottle, the filter can be cleaned and replaced, and the filtering effect of the filter can be avoided due to long-term use. This ensures that the filtered cell fluid is of good quality.

Description of drawings

Fig. 1 is a structural representation of the utility model;

Fig. 2 is the structural representation of filter screen of the present utility model;

Fig. 3 is a structural schematic diagram of the installation port of the present invention.

In the figure: 1, bottle cap, 2, collar, 3, fixed plate, 4, first connecting pipe, 5, hook, 6, effusion cylinder, 7, valve, 8, fixed ring, 9, handle, 10, Catheter, 11, suction head, 12, clamping head, 13, cell solution placement bottle, 14, stable seat, 15, support plate, 16, support rod, 17, vertical plate, 18, installation cap, 19, fixed Seat, 20, cross bar, 21, vacuum device, 22, the second connecting pipe, 23, fixed block, 24, hold cover firmly, 25, place seat, 26, filter screen, 27, pressing plate, 28, telescopic spring, 29 , installation port, 30, sealing sleeve.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

Please refer to Figures 1-3, the utility model provides a technical solution: a collection device for cell culture fluid, including a cell fluid placement bottle 13, a support plate 15 is installed inside the cell fluid placement bottle 13, and the top of the support plate 15 The left and right sides are all fixedly installed with vertical plate 17, and the outer wall of vertical plate 17 is provided with mounting opening 29, can realize the installation to chuck 12 by mounting opening 29, thereby fixed plate 3 is fixed, the outside of vertical plate 17 is installed with Cross bar 20 can be fixed to vertical plate 17 by cross bar 20, and the outer side of cross bar 20 is fixedly installed with fixing seat 19, and the top left and right sides of support plate 15 is all fixedly installed with stabilizing seat 14, and the top of stabilizing seat 14 is fixedly installed There is a support rod 16, the installation of the mounting cap 18 can be realized by the support rod 16, thereby ensuring the stability of the placement of the fixed plate 3, the top of the support rod 16 is fixedly installed with the mounting cap 18, and the top of the mounting cap 18 is fixedly installed with the fixed plate 3 , the interior of the fixed plate 3 is fixedly installed with a filter screen 26, and the left and right sides of the fixed plate 3 are all fixedly equipped with a placement seat 25, and the inside of the placement seat 25 is equipped with a telescopic spring 28, and the telescopic spring 28 is a spiral telescopic spring, and the coefficient of elasticity is 12N/CM, a pressing plate 27 is fixedly installed on the outside of the telescopic spring 28, and the telescopic spring 28 can be pressed by the pressing plate 27 to expand and contract. The chuck 12 runs through the outer wall of the placement seat 25, the chuck 12 runs through the installation port 29, the top of the cell solution bottle 13 is provided with a bottle cap 1, and the left and right sides of the top of the bottle cap 1 are fixedly equipped with stabilizing sleeves 24, and the cell liquid The left and right sides of the inner top of the bottle 13 are respectively equipped with a second connecting pipe 22 and a first connecting pipe 4, and the outer walls of the second connecting pipe 22 and the first connecting pipe 4 are sleeved with a collar 2, through which the collar 2 can To increase the stability of the placement of the second connecting pipe 22 and the first connecting pipe 4, a fixing block 23 is fixedly installed on the outside of the collar 2, and the collar 2 can be fixed by the fixing block 23. The second connecting pipe 22 and the first connecting pipe 22 are connected The tubes 4 all run through the outer wall of the cell fluid storage bottle 13 and the bottle cap 1, and the joints of the second connecting tube 22 and the first connecting tube 4 with the cell fluid storage bottle 13 are fixedly equipped with a sealing sleeve 30, through which the sealing sleeve 30 can increase the The tightness of the connection between the second connecting pipe 22 and the first connecting pipe 4 and the cell solution storage bottle 13, the bottom end of the second connecting pipe 22 is fixedly equipped with a vacuum device 21, and the bottom end of the first connecting pipe 4 is fixedly installed with a liquid accumulation Cylinder 6, a handle 9 is fixedly installed on the left outer wall of the effusion cylinder 6, and a fixing ring 8 is fixedly installed on the right outer wall of the effusion cylinder 6. The fixing ring 8 can realize the fixing of the effusion cylinder 6, and the cell solution is placed in the bottle. The top right side of 13 is fixedly installed with hook 5, can realize the hanging of fixed ring 8 by hook 5, and fixed ring 8 matches with hook 5, and valve 7 is installed on the front outer wall of effusion cylinder 6, and the bottom end of valve 7 is fixed A catheter 10 is installed, and a liquid suction head 11 is fixedly installed at the bottom end of the catheter 10 .

When the filter screen 26 needs to be cleaned and replaced, the first connecting tube 4 and the second connecting tube 22 can be pulled upwards, and the first connecting tube 4 and the second connecting tube 22 can be taken out from the cell solution placement bottle 13, and the bottle can be rotated Cap 1, the bottle cap 1 can be removed, by pushing the chuck 12 inward, the chuck 12 pushes the pressure plate 27 to move inward, the pressure plate 27 presses the telescopic spring 28 to expand and contract, and the chuck 12 is installed on the outer wall of the vertical plate 17 Pull out from the mouth 29, pull the fixed plate 3 upwards, the fixed plate 3 drives the installation cap 18 at the bottom away from the support rod 16, take the fixed plate 3 out of the cell liquid and place it inside the bottle 13, and then filter the filter 26 inside the fixed plate 3 Clean and replace.

In the description of the present utility model, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "one side", " The orientation or positional relationship indicated by "top", "inner", "front", "central", "both ends" etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the utility model and simplifying the description , rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the utility model.

In this utility model, unless otherwise clearly specified and limited, terms such as "installation", "setting", "connection", "fixation" and "rotation" should be understood in a broad sense, for example, it can be a fixed connection, It can also be a detachable connection, or integrated; it can be a mechanical connection, it can also be an electrical connection; it can be directly connected, or indirectly connected through an intermediary, and it can be the internal communication of two components or the interaction of two components Unless otherwise clearly defined, those skilled in the art can understand the specific meanings of the above terms in the present utility model according to specific situations.

Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes and modifications can be made to these embodiments without departing from the principle and spirit of the present invention , replacements and modifications, the scope of the present utility model is defined by the appended claims and their equivalents.

Claims (5)

1. a kind of collection device of cell culture fluid, including cell liquid place bottle (13), it is characterised in that：The cell liquid is placed The inside of bottle (13) is provided with supporting plate (15), and the top left and right sides of the supporting plate (15) is installed with riser (17), the outer wall of the riser (17) is provided with the outside of installing port (29), the riser (17) and is provided with cross bar (20), the horizontal stroke Fixed seat (19) is installed with the outside of bar (20), the top left and right sides of the supporting plate (15) is installed with stabilization Seat (14), the top of the stabilizing base (14) is installed with support bar (16), and the top of the support bar (16) is fixedly mounted There is mounting cap (18), the top of the mounting cap (18) is installed with fixed plate (3), and the inside of the fixed plate (3) is fixed Screen pack (26) is installed, the left and right sides of the fixed plate (3) is installed with placement rack (25), the placement rack (25) Inside be provided with the outside of adjustable spring (28), the adjustable spring (28) and be installed with pressing plate (27), the pressing plate (27) dop (12) is installed with the outside of, the dop (12) runs through the outer wall of placement rack (25), and the dop (12) is passed through Wear installing port (29), the top that the cell liquid places bottle (13) is provided with bottle cap (1), top of the bottle cap (1) or so two Side, which is installed with, holds set (24), and the inner tip left and right sides that the cell liquid places bottle (13) is separately installed with second Connecting tube (22) and the first connecting tube (4), second connecting tube (22) and the first connecting tube (4) place bottle through cell liquid (13) and bottle cap (1) outer wall, the bottom of second connecting tube (22) is installed with vacuum plant (21), and described first connects The bottom of adapter (4) is installed with hydrops cylinder (6), and the front outer wall of the hydrops cylinder (6) is provided with valve (7), the valve The bottom of door (7) is installed with catheter (10), and the bottom of the catheter (10) is installed with fluid suction head (11).

2. a kind of collection device of cell culture fluid according to claim 1, it is characterised in that：Second connecting tube (22) it is socketed with the outside of the collar (2), the collar (2) with the outer wall of the first connecting tube (4) and is installed with fixed block (23)。

3. a kind of collection device of cell culture fluid according to claim 1, it is characterised in that：The hydrops cylinder (6) Outer right wall is installed with retainer ring (8), and the right side top that the cell liquid places bottle (13) is installed with hook (5), The retainer ring (8) matches with hook (5).

4. a kind of collection device of cell culture fluid according to claim 1, it is characterised in that：Second connecting tube (22) place the junction of bottle (13) with the first connecting tube (4) and cell liquid and be installed with sealing shroud (30).

5. a kind of collection device of cell culture fluid according to claim 1, it is characterised in that：The hydrops cylinder (6) Left side outer wall is installed with handle (9).