CN206607227U - A kind of collection device of cell culture fluid - Google Patents

Abstract

The utility model discloses a collection device for cell culture fluid, which comprises a collection cylinder. An end cap is installed on the top of the collection cylinder. A bottom cylinder is fixedly installed, and a top cylinder is installed on the top of the bottom cylinder, and filtering devices are installed on the outer walls of the top cylinder and the bottom cylinder, and the inner ring seat is connected with the placement seat through a collar. The device for collecting cell culture fluid filters impurities following the cell culture fluid through the cooperation of the top cylinder, the bottom cylinder and the placement seat, and through the inner nylon filter, the inner metal filter and the outer metal filter, which improves the efficiency of cell culture. To ensure the purity of the liquid after collection, the collar rotates on the inner ring seat through the collar when the placement seat is turned, so as to avoid the inner cell culture liquid from pouring due to the placement seat driving the inner collection barrel to rotate when taking the inner collection barrel, which is convenient for taking out Take the collected cell culture fluid.

Description

A device for collecting cell culture fluid

technical field

The utility model relates to the technical field of experimental instruments, in particular to a collection device for cell culture fluid.

Background technique

In the cell culture of biomedical and chemical experiments, a large number of cells need to be cultured and expanded. A large amount of cell culture liquid will be produced in the cell expansion project. These culture liquids are either collected for harmless treatment, or recycled and reused. The aseptic conditions of cell culture operation and the cleanliness of the surrounding area are relatively high. A little carelessness in the operation will contaminate the cells, affect the normal operation of the experimenter, and cause the experiment to fail. There are various collection devices for cell culture fluid. But most of them are to improve the collection effect, and do not improve the filtration effect of the collected cell culture fluid, and there is no convenience for taking the collected cell culture fluid. In the existing cell culture fluid collection device, for example, the application number is The utility model patent of 201520765396.9 includes a cell liquid container, a pipette head connected to the cell liquid container, and a vacuum device that generates negative pressure in the cell liquid container. The upper opening of the cell liquid container is equipped with a sealing plug, and the cell liquid container The container and the pipette head are communicated through the sealing plug through the first hose, and the hose is equipped with an operating handle. Although this patent facilitates the collection, it does not improve the filtration effect of the collected cell culture solution, and It is not convenient to take the collected cell culture fluid.

Utility model content

The purpose of this utility model is to provide a collection device for cell culture fluid, so as to solve the problems raised in the above-mentioned background technology.

In order to achieve the above object, the utility model provides the following technical solutions: a collection device for cell culture fluid, including a collection cylinder, an end cap is installed on the top of the collection cylinder, and a liquid inlet pipe is connected through the inside of the end cap, The outer side of the liquid inlet pipe is provided with a sleeve, the top end of the sleeve is installed with a gland, the outer wall of the sleeve and the junction of the end cap are provided with a sealing ring, and the inner bottom of the collection tube is fixedly installed There is a bottom cylinder, a top cylinder is installed on the top of the bottom cylinder, and the outer walls of the top cylinder and the bottom cylinder are respectively equipped with a second filter device and a first filter device, and the first filter device and the second filter device both include Net frame, the inside center of the net frame is equipped with an inner nylon filter screen, the left and right ends of the inner nylon filter screen are respectively equipped with an inner metal filter screen and an outer metal filter screen, and the bottom end of the collection cylinder is provided with The placement seat, the placement seat is connected with the collection tube through a threaded sleeve, the inner side of the placement seat is provided with an inner collection tube, the inner collection tube runs through the bottom tube, and the top of the outer wall of the inner collection tube is sleeved with an outer rubber sleeve , the inner wall of the placement seat is provided with an inner ring seat, the inner ring seat is connected to the placement seat through a collar, the inner wall of the inner ring seat is provided with a strip groove, and the outer wall of the inner collection tube is provided with a strip shape plate, the strip-shaped plate is matched with the strip-shaped groove.

Preferably, a push-pull rod is connected through the outside of the bottom end of the collection tube, a sealing rubber tube is provided at the connection between the push-pull rod and the collection tube, and an annular cover is installed on the outside of the seal rubber tube, and the push-pull rod A metal ring is installed on the top, and the outer wall of the metal ring is tightly wrapped with a leather plug, and the leather plug is matched with the collection tube and the bottom tube, and the bottom edge ring is installed at the bottom end of the outer wall of the push-pull rod.

Preferably, a triangular pyramid is installed at the inner bottom end of the top cylinder.

Preferably, a sealing groove is provided on the outer side of the bottom end of the end cover, the top cylinder matches the sealing groove, and a sealing rubber pad is attached to the inner wall of the sealing groove.

Preferably, the outer wall of the placement seat is tightly wrapped with a non-slip rubber sleeve.

Compared with the prior art, the beneficial effect of the utility model is: the collection device of the cell culture fluid, through the cooperation of the top cylinder, the bottom cylinder and the placement seat, the cell culture fluid enters the collection cylinder and falls into the top cylinder, and passes The filter device falls into the inside of the collection cylinder, and after accumulation, it passes through the filter device on the outer wall of the bottom cylinder and enters the inner collection cylinder in the bottom cylinder for collection, and then passes through the inner nylon filter, the inner metal filter and the outer metal filter to follow the cell culture medium Filter the impurities to improve the purity of the cell culture medium after collection. When taking the inner collection tube, turn the placement seat to make it rotate on the collection tube through the threaded sleeve thread. At the same time, because the inner collection tube contains a certain The liquid of the cell culture medium has a certain weight. When the placement seat is rotated, the collar is rotated on the inner ring seat, so that the placement seat is in a certain stable state during rotation, and the placement seat is prevented from driving the inner ring when the inner collection tube is taken. The rotation of the collection cylinder causes the cell culture fluid inside to pour out, which facilitates the taking of the collected cell culture fluid.

Description of drawings

Fig. 1 is the structural representation of the utility model;

Fig. 2 is the structural representation of the first filtering device of the present utility model;

Fig. 3 is a structural schematic diagram of the placement seat of the present utility model;

Fig. 4 is a structural schematic diagram of the strip plate of the present invention.

In the figure: 1. Sealing rubber pad, 2. End cap, 3. Gland, 4. Liquid inlet pipe, 5. Sleeve, 6. Sealing ring, 7. Sealing groove, 8. Collection cylinder, 9. Triangular cone , 10, the first filtering device, 101, the inner metal filter screen, 102, the inner nylon filter screen, 103, the outer metal filter screen, 104, the grid frame, 11, the inner collection cylinder, 12, the leather plug, 13, the sealing rubber cylinder , 14, push-pull rod, 15, second filter device, 16, top cylinder, 17, bottom cylinder, 18, outer rubber sleeve, 19, metal ring, 20, ring cover, 21, bottom edge ring, 22, thread sleeve, 23, place seat, 24, collar, 25, anti-slip rubber cover, 26, inner ring seat, 27, strip plate, 28, strip groove.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

Please refer to Figures 1-4, the utility model provides a technical solution: a collection device for cell culture fluid, including a collection cylinder 8, an end cap 2 is installed on the top of the collection cylinder 8, and a bottom end outside the end cap 2 is provided The sealing groove 7, the top cylinder 16 is matched with the sealing groove 7, the inner wall of the sealing groove 7 is pasted with a sealing rubber pad 1, and the inside of the end cover 2 is connected with a liquid inlet pipe 4, and the cell culture liquid is collected through the liquid inlet pipe 4 Inside the cylinder 8, a sleeve 5 is provided on the outside of the liquid inlet pipe 4, a gland 3 is installed on the top of the sleeve 5, a sealing ring 6 is provided at the joint between the outer wall of the sleeve 5 and the end cover 2, and the sleeve 5 is socketed On the outside of the liquid inlet pipe 4, it is fixed by the gland 3 and the sealing ring 6, and the sealing performance is improved. The inner bottom of the collection tube 8 is fixedly installed with a bottom tube 17, and the top end of the bottom tube 17 is installed with a top tube 16 , the inner bottom of the top cylinder 16 is equipped with a triangular cone 9, the outer wall of the top cylinder 16 and the bottom cylinder 17 are respectively equipped with a second filter device 15 and a first filter device 10, the first filter device 10 and the second filter device 15 Both include a grid frame 104, the inside center of the grid frame 104 is equipped with an inner nylon filter screen 102, and the left and right ends of the inner nylon filter screen 102 are respectively equipped with an inner metal filter screen 101 and an outer metal filter screen 103, and the cell culture solution enters and collects Fall into the top cylinder 16 after the cylinder 8, and fall into the inside of the collection cylinder 8 through the filter device 10, and after accumulation, enter the inner collection cylinder 11 in the bottom cylinder 17 through the filter device 10 on the outer wall of the bottom cylinder 17 for collection. When the culture liquid passes through the filter device 10, the impurities following the cell culture liquid are filtered through the inner nylon filter screen 102, the inner metal filter screen 101 and the outer metal filter screen 103, which improves the purity of the cell culture liquid after collection, and the collection cylinder 8 The bottom end of the base is provided with a placement seat 23, the outer wall of the placement seat 23 is tightly wrapped with a non-slip rubber sleeve 25, the placement seat 23 is connected with the collection tube 8 through a threaded sleeve 22, and the inner side of the placement seat 23 is provided with an inner collection tube 11, and the inner collection tube 11 runs through the bottom cylinder 17, the top of the outer wall of the inner collection tube 11 is sleeved with an outer rubber sleeve 18, the inner wall of the placement seat 23 is provided with an inner ring seat 26, and the inner ring seat 26 is connected with the placement seat 23 through a collar 24, and the inner ring seat The inwall of 26 is provided with strip groove 28, and the outer wall of inner collection cylinder 11 is provided with strip plate 27, and strip plate 27 matches with strip groove 28, and interior collection cylinder 11 is placed in the placement seat 23, and strip plate 27 is inserted in the bar-shaped groove 28, improves the stability after placing, avoids inner collecting tube 11 to move, and rotates placement seat 23, makes it be threadedly connected on the collecting tube 8 by threaded sleeve 22, simultaneously because adornment is contained in the inner collecting tube 11 The liquid of a certain cell culture fluid has a certain weight, and when the placement seat 23 is rotated, it is rotated on the inner ring seat 26 by the collar 24, so that the placement seat 23 is in a certain stable state when rotating, and it is avoided to be collected in the taking place. When the tube 11 is in place, the placement seat 23 drives the inner collection tube 11 to rotate, causing the cell culture solution inside to pour. The outside of the bottom end of the collection tube 8 is connected with a push-pull rod 14. Sealing rubber cylinder 13, the outer side of sealing rubber cylinder 13 is installed An annular cover 20 is installed, a metal ring 19 is installed on the top of the push-pull rod 14, the outer wall of the metal ring 19 is tightly wrapped with a leather plug 12, the leather plug 12 is matched with the collection tube 8 and the bottom tube 17, and the bottom end of the outer wall of the push-pull rod 14 The bottom edge ring 21 is installed, and the push-pull rod 14 is manually pushed to drive the metal ring 19 and the belt 12 to move upwards, and the residual cell culture solution is pushed to the filter device 10 on the bottom tube 17 and then flows into the inner collection tube 11, avoiding Collect the residual cell culture samples in the cylinder 8 .

The cell culture solution enters the collection tube 8 through the liquid inlet pipe 4, the sleeve 5 is sleeved on the outside of the liquid inlet pipe 4, and is fixed by the gland 3 and the sealing ring 6, and the sealing performance is improved, and the cell culture solution enters the collection tube 8. Fall into the top cylinder 16 after the cylinder 8, and fall into the inside of the collection cylinder 8 through the filter device 10, and after accumulation, enter the inner collection cylinder 11 in the bottom cylinder 17 through the filter device 10 on the outer wall of the bottom cylinder 17 for collection. When the culture solution passes through the filter device 10, the impurities following the cell culture solution are filtered through the inner nylon filter screen 102, the inner metal filter screen 101 and the outer metal filter screen 103, which improves the purity of the cell culture solution after collection. When the inner collection tube 11 is rotated, the placement seat 23 is threaded to rotate on the collection tube 8 through the threaded sleeve 22. Simultaneously, the inner collection tube 11 has a certain weight due to the liquid containing a certain amount of cell culture fluid. When the seat 23 is rotated on the inner ring seat 26 by the collar 24, the placement seat 23 is in a certain stable state when rotating, so as to prevent the inner collection tube 11 from being driven by the placement seat 23 to rotate when the inner collection tube 11 is taken. Causing the internal cell culture fluid to spill.

In the description of the present utility model, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "one side", " The orientation or positional relationship indicated by "top", "inner", "front", "central", "both ends" etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the utility model and simplifying the description , rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the utility model.

In addition, the terms "first", "second", "third" and "fourth" are used for descriptive purposes only, and should not be understood as indicating or implying relative importance or implicitly specifying the number of indicated technical features, Thus, a feature defined as "first", "second", "third" and "fourth" may explicitly or implicitly include at least one of the features.

In this utility model, unless otherwise clearly specified and limited, terms such as "installation", "setting", "connection", "fixation" and "rotation" should be understood in a broad sense, for example, it may be a fixed connection, It can also be detachable connection, or integrated; it can be mechanical connection or electrical connection; it can be directly connected or indirectly connected through an intermediary, and it can be the internal communication of two components or the interaction of two components Unless otherwise clearly defined, those skilled in the art can understand the specific meanings of the above terms in the present utility model according to specific situations.

Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes and modifications can be made to these embodiments without departing from the principle and spirit of the present invention , replacements and modifications, the scope of the present utility model is defined by the appended claims and their equivalents.

Claims (5)

1. a kind of collection device of cell culture fluid, including surge drum（8）, the surge drum（8）Top end cap is installed （2）, the end cap（2）Inside through being connected with liquid-in pipe（4）, it is characterised in that：The liquid-in pipe（4）Outside provided with set Cylinder（5）, the sleeve（5）Top gland is installed（3）, the sleeve（5）Outer wall and end cap（2）Junction provided with close Seal ring（6）, the surge drum（8）Inner bottom be installed with bottom cylinder（17）, the bottom cylinder（17）Top top is installed Cylinder（16）, the top cylinder（16）With bottom cylinder（17）Outer wall be separately installed with the second filter（15）With the first filter （10）, first filter（10）With the second filter（15）Include rack（104）, the rack（104）It is interior Side center is provided with interior nylon filter（102）, the interior nylon filter（102）Left and right ends be separately installed with interior gold Belong to screen pack（101）With outer metal filter screen（103）, the surge drum（8）Bottom be provided with placement rack（23）, the placement rack （23）Pass through thread bush（22）With surge drum（8）It is connected, the placement rack（23）Inner side be provided with interior surge drum（11）, it is described interior Surge drum（11）Through bottom cylinder（17）, the interior surge drum（11）Outer wall top be socketed with outer rubber set（18）, it is described to place Seat（23）Inwall be provided with interior ring seat（26）, the interior ring seat（26）Pass through collar（24）With placement rack（23）It is connected, it is described interior Ring seat（26）Inwall be provided with bar-shaped trough（28）, the interior surge drum（11）Outer wall be provided with stripe board（27）, the stripe board （27）With bar-shaped trough（28）Match.

2. a kind of collection device of cell culture fluid according to claim 1, it is characterised in that：The surge drum（8）'s Basolateral, which runs through, is connected with pull bar（14）, the pull bar（14）With surge drum（8）Junction provided with caulking gum cylinder （13）, the caulking gum cylinder（13）Outside ring cover is installed（20）, the pull bar（14）Top metal is installed Ring（19）, the becket（19）Outer wall tight have leather plug（12）, the leather plug（12）With surge drum（8）With bottom cylinder （17）It is engaged, the pull bar（14）Outer wall bottom base ring is installed（21）.

3. a kind of collection device of cell culture fluid according to claim 1, it is characterised in that：The top cylinder（16）It is interior Portion bottom is provided with triangular pyramids（9）.

4. a kind of collection device of cell culture fluid according to claim 1, it is characterised in that：The end cap（2）Bottom End outside is provided with seal groove（7）, the top cylinder（16）With seal groove（7）Match, the seal groove（7）Inwall be fitted with it is close Potting pad（1）.

5. a kind of collection device of cell culture fluid according to claim 1, it is characterised in that：The placement rack（23）'s Outer wall tight has anti-slip rubber set（25）.