CN206862926U - A kind of contained network support film prepares servicing unit - Google Patents
A kind of contained network support film prepares servicing unit Download PDFInfo
- Publication number
- CN206862926U CN206862926U CN201720414143.6U CN201720414143U CN206862926U CN 206862926 U CN206862926 U CN 206862926U CN 201720414143 U CN201720414143 U CN 201720414143U CN 206862926 U CN206862926 U CN 206862926U
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- CN
- China
- Prior art keywords
- liquid bottle
- liquid
- holding cup
- ventilation
- ventilation connector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Servicing unit is prepared the utility model discloses a kind of contained network support film, including liquid bottle, also include the liquid holding cup of open-top, the bottom of liquid holding cup is provided with ventilation connector, the catheter being connected with the bottom of liquid holding cup is provided with ventilation connector, ventilation connector is stretched out in the end of catheter；Ventilation nozzle is provided with described ventilation connector, ventilation nozzle is connected by tracheae with pulsometer；Described ventilation connector sealing coordinates when on liquid bottle, and the end of catheter is close with the bottom of liquid bottle but does not contact.Device provided by the utility model, it is easy to operate, during preparing contained network and supporting film, it can be forced the fluid into by pulsometer so as to flood glass sheet partial in liquid holding cup, then liquid is at the uniform velocity flowed back in liquid bottle by the slow deflation of pulsometer again, take out glass plate, contained network in uniform thickness can be obtained and support film, contained network is improved and support film quality, while improve follow-up electron microscopic observation and image quality.
Description
Technical field
The utility model belongs to field of biochemistry detection, is related to contained network and supports film, and in particular to a kind of contained network supports film
Prepare servicing unit.
Background technology
In transmission electron microscope ultramicrotomy, contained network supports film to be widely used for carrying ultra-thin section and particulate samples,
Formvar films are not easy to be broken up by electron beam because mechanical strength is good, thus are widely used, but Formvar film preparations get up compared with
For difficulty, it is even to easily cause non-uniform film thickness, or even a lot of ripple occurs.The preparation method of Formvar films was mostly in the past
In standing a moment in the Formvar solution for being 0.3% by the glass plate insertion mass concentration of wash clean, then slowly at the uniform velocity take out,
Thin film will be formed on a glass by drying slightly again, and film is carved into a square at the edge of film with sharp blade or syringe needle
Shape, by glass plate, gently oblique cutting enters to fill with the container of sterilized water, film is separated simultaneously with glass plate by water surface tension effect
Swim on the water surface, finally by the support film transfer made to contained network.
The difficulty of Formvar film preparations is that when slowly at the uniform velocity extracting glass plate from Formvar solution by hand, hand holds
Easily shake, it is difficult to accomplish at the uniform velocity, while the speed extracted also is not easily controlled.The result is that manufactured support film thickness is not easy
Hold, it is in uneven thickness, or even there is a lot of ripple, influence follow-up electron microscopic observation and image quality.
The content of the invention
In view of the deficienciess of the prior art, the purpose of this utility model is, there is provided a kind of contained network supports the preparation of film
Servicing unit, overcome during existing contained network supports film preparation, support that non-uniform film thickness is even caused by during manual extraction glass plate,
Even there is the technical problem of the follow-up electron microscopic observation of the influences such as a lot of ripple and image quality.
In order to solve the above-mentioned technical problem, the utility model, which adopts the following technical scheme that, is achieved：
A kind of contained network supports the liquid holding cup for preparing servicing unit, including liquid bottle, including open-top of film, liquid holding cup
Bottom be provided with ventilation connector, be provided with the catheter being connected with the bottom of liquid holding cup, catheter in connector of ventilating
End stretch out ventilation connector；
Ventilation nozzle is provided with described ventilation connector, ventilation nozzle is connected by tracheae with pulsometer, connector of ventilating
For with liquid bottle sealing coordinate connect, by pulsometer into liquid bottle inflating pressure；
Described ventilation connector sealing coordinates when on liquid bottle, the end of catheter and the bottom of liquid bottle it is close but
Do not contact.
The utility model also has following distinguishing feature：
Current stabilization orifice plate is additionally provided with described liquid holding cup.
Assembly connection is carried out using grinding head between described ventilation connector and liquid bottle.
The top that described liquid holding cup opens is configured with top cover.
For described ventilation connector with being also equipped with clamper on liquid bottle, described clamper includes handle attachment part,
One end of handle attachment part is provided with buckle, and the other end of handle attachment part is provided with lower buckle, and upper buckle is used to be clamped in
On the first thin neck between liquid holding cup and ventilation connector, lower buckle is used to be clamped in second thin neck of the liquid bottle close to bottleneck
In portion.
The second described thin neck is formed by the outer wall of liquid bottle with the flange for being positioned close to liquid bottle bottleneck.
Described upper buckle and the inwall of lower buckle are disposed as dentation.
Described pulsometer uses manual pneumatic pump or electropneumatic pump.
Described liquid bottle is conical flask.
The utility model compared with prior art, has the following technical effect that：
Contained network provided by the utility model supports the servicing unit for preparing of film, easy to operate, and film is supported preparing contained network
During, it can be forced the fluid into by pulsometer in liquid holding cup so as to flood glass sheet partial, then pass through pulsometer again
Slowly deflate, liquid is at the uniform velocity flowed back in liquid bottle, take out glass plate, contained network in uniform thickness can be obtained and support film, improved
Contained network supports film quality, while improves follow-up electron microscopic observation and image quality.
Brief description of the drawings
Fig. 1 is pulsometer when being manual pneumatic pump, overall structure diagram of the present utility model.
Fig. 2 is pulsometer when being electropneumatic pump, overall structure diagram of the present utility model.
Fig. 3 is the assembly relation schematic diagram of liquid holding cup and connector of ventilating.
Fig. 4 is the part schematic diagram of upper buckle.
The implication of each label is in figure：1- liquid bottles, 2- liquid holding cups, 3- ventilation connectors, 4- catheters, 5- ventilations
Mouth, 6- tracheaes, 7- pulsometers, 8- current stabilization orifice plates, 9- top covers, 10- clampers, the first thin necks of 11-, the second thin necks of 12-,
13- flanges, 14- glass plates；
(10-1)-handle attachment part, (10-2)-upper buckle, (10-3)-lower buckle.
Explanation is further explained in detail to particular content of the present utility model with reference to embodiments.
Embodiment
Specific embodiment of the utility model given below is, it is necessary to which explanation is that the utility model is not limited to following tool
Body embodiment, all equivalents done on the basis of technical scheme each fall within the scope of protection of the utility model.
Embodiment：
Above-mentioned technical proposal is deferred to, as shown in Figures 1 to 4, the present embodiment provides the preparation auxiliary that a kind of contained network supports film
Device, including liquid bottle 1, include the liquid holding cup 2 of open-top, and the bottom of liquid holding cup 2 is provided with ventilation connector 3, ventilation
The catheter 4 being connected with the bottom of liquid holding cup 2 is provided with connector 3, ventilation connector 3 is stretched out in the end of catheter 4；
Ventilation nozzle 5 is provided with described ventilation connector 3, ventilation nozzle 5 is connected by tracheae 6 with pulsometer 7, and ventilation connects
Joint 3 be used for liquid bottle 1 sealing coordinate connects, by pulsometer 7 into liquid bottle 1 inflating pressure；
The described sealing of ventilation connector 3 coordinates when on liquid bottle 1, and the end and the bottom of liquid bottle 1 of catheter 4 are leaned on
Closely but do not contact.
Specifically, current stabilization orifice plate 8 is additionally provided with described liquid holding cup 2, in order to which the liquid level of solution in liquid holding cup 2 can be put down
Steady uniform descent.
As a kind of preferred scheme, assembling company is carried out using grinding head between described ventilation connector 3 and liquid bottle 1
Connect, so that it is guaranteed that being tightly connected between ventilation connector 3 and liquid bottle 1.
Specifically, the top that described liquid holding cup 2 opens is configured with top cover 9, prevent dust impurity etc. from falling in liquid holding cup 2
On glass plate 14.
Specifically, described ventilation connector 3, with being also equipped with clamper 10 on liquid bottle 1, described clamper 10 wraps
Handle attachment part 10-1 is included, handle attachment part 10-1 one end is provided with buckle 10-2, the handle attachment part 10-1 other end
Lower buckle 10-3 is installed, upper buckle 10-2 is used for the first thin neck 11 for being clamped in liquid holding cup 2 between connector 3 of ventilating
On, lower buckle 10-3 is used to be clamped in second thin neck 12 of the liquid bottle 1 close to bottleneck, it is ensured that ventilation connector 3 and dress liquid
The close connection of the sealing all the time of bottle 1.
Specifically, the second described thin neck 12 by liquid bottle 1 outer wall and be positioned close to the flange of the bottleneck of liquid bottle 1
13 form, convenient lower buckle 10-3 clamping.
As a kind of preferred scheme, described upper buckle 10-2 and lower buckle 10-3 inwall are disposed as dentation, convenient
The dismounting of clamper 10.
Specifically, described pulsometer 7 uses manual pneumatic pump or electropneumatic pump, in order under different operating environment
Different pulsometers 7 is selected, it is easy to operation.
As a kind of preferred scheme, described liquid bottle 1 is conical flask, stability easy to operation, can also be held more
Solution.
The utility model is in use, the film making solution used is for the mass concentration that pure dichloroethylene or chloroform are made into
0.3% Formvar solution, described Formvar chemical composition is polyvinyl formal.In described liquid bottle 1
Load enough Formvar solution, described ventilation connector 3 is then sealed into cooperation on liquid bottle 1, glass plate 14 is hung down
Directly be placed on current stabilization orifice plate 8, liquid holding cup 2 open top top cover 9, by pulsometer 7 with ventilation connector 3 on ventilation
Mouth 5 is tightly connected, and opens pulsometer 7, air is pressed into liquid bottle 1, compressed air is by the Formvar solution pressures in liquid bottle 1
Enter in liquid holding cup 2, solution flows through current stabilization orifice plate 8 and floods glass sheet partial 14, then adjusts pulsometer 7 and slowly deflate, and makes liquid holding cup 2
The slow uniform descent of interior solution, is flowed back in liquid bottle 1, then takes out glass plate 14, with sharp blade or syringe needle after drying
Film is carved into a rectangle at the edge of film, gently oblique cutting enters to fill with the container of sterilized water by glass plate 14, by the table of water
Face tension force effect makes film separate and swim on the water surface with glass plate 14, finally supports film transfer to contained network the contained network made
On.
Claims (9)
1. a kind of contained network support film prepares servicing unit, including liquid bottle (1), it is characterised in that also including open-top
Liquid holding cup (2), the bottom of liquid holding cup (2) are provided with ventilation connector (3), are provided with ventilation connector (3) and liquid holding cup (2)
The catheter (4) that is connected of bottom, ventilation connector (3) is stretched out in the end of catheter (4)；
Ventilation nozzle (5) is provided with described ventilation connector (3), ventilation nozzle (5) is connected by tracheae (6) with pulsometer (7),
Ventilation connector (3) be used for liquid bottle (1) sealing coordinate connects, by pulsometer (7) into liquid bottle (1) inflating pressure；
Described ventilation connector (3) sealing coordinates when on liquid bottle (1), the end of catheter (4) and the bottom of liquid bottle (1)
Portion is close but does not contact.
2. device as claimed in claim 1, it is characterised in that be additionally provided with current stabilization orifice plate (8) in described liquid holding cup (2).
3. device as claimed in claim 1, it is characterised in that used between described ventilation connector (3) and liquid bottle (1)
Grinding head carries out assembly connection.
4. device as claimed in claim 1, it is characterised in that the open top of described liquid holding cup (2) is configured with top cover
(9)。
5. device as claimed in claim 1, it is characterised in that described ventilation connector (3) on liquid bottle (1) with also installing
There is clamper (10), described clamper (10) includes handle attachment part (10-1), one end installation of handle attachment part (10-1)
There is upper buckle (10-2), the other end of handle attachment part (10-1) is provided with lower buckle (10-3), and upper buckle (10-2) is used to press from both sides
Hold on the first thin neck (11) between liquid holding cup (2) and ventilation connector (3), lower buckle (10-3) is used to be clamped in dress liquid
Bottle (1) is on the second thin neck (12) of bottleneck.
6. device as claimed in claim 5, it is characterised in that the second described thin neck (12) by liquid bottle (1) outer wall
With flange (13) formation for being positioned close to liquid bottle (1) bottleneck.
7. device as claimed in claim 5, it is characterised in that described upper buckle (10-2) and the inwall of lower buckle (10-3)
It is disposed as dentation.
8. device as claimed in claim 1, it is characterised in that described pulsometer (7) uses manual pneumatic pump or electronic gas
Press pump.
9. device as claimed in claim 1, it is characterised in that described liquid bottle (1) is conical flask.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720414143.6U CN206862926U (en) | 2017-04-19 | 2017-04-19 | A kind of contained network support film prepares servicing unit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720414143.6U CN206862926U (en) | 2017-04-19 | 2017-04-19 | A kind of contained network support film prepares servicing unit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206862926U true CN206862926U (en) | 2018-01-09 |
Family
ID=60817810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720414143.6U Expired - Fee Related CN206862926U (en) | 2017-04-19 | 2017-04-19 | A kind of contained network support film prepares servicing unit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206862926U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346389A (en) * | 2019-06-20 | 2019-10-18 | 中国人民解放军南部战区总医院 | One kind being film-made instrument for transmission electron microscope sample |
-
2017
- 2017-04-19 CN CN201720414143.6U patent/CN206862926U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346389A (en) * | 2019-06-20 | 2019-10-18 | 中国人民解放军南部战区总医院 | One kind being film-made instrument for transmission electron microscope sample |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180109 Termination date: 20180419 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |