CN206308369U - The equipment for promoting cell to discharge vesica - Google Patents
The equipment for promoting cell to discharge vesica Download PDFInfo
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- CN206308369U CN206308369U CN201621145718.0U CN201621145718U CN206308369U CN 206308369 U CN206308369 U CN 206308369U CN 201621145718 U CN201621145718 U CN 201621145718U CN 206308369 U CN206308369 U CN 206308369U
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Abstract
The utility model is applied to cell technology field, there is provided a kind of equipment that promotion cell discharges vesica, the equipment includes：Cell delivery device, is connected with cell release vesica device, by the cell delivery of the concentration after culture to cell release vesica device；Cell discharges vesica device, including container and irradiation light, and the irradiation light is fixed on the container, and carries out the irradiation of the scheduled time to the cell under the conditions of default, makes the cell release vesica.Whereby, the utility model provides special equipment inducing cell apoptosis to realize, and makes the batch operation of promotion cell release vesica more convenient.
Description
Technical field
The utility model is related to cell processing techniques field, more particularly to a kind of equipment for promoting cell to discharge vesica.
Background technology
At entitled " a kind of tumor chemotherapeutic medicinal preparation and preparation method thereof " of Application No. CN102302784A
Chinese patent disclose a kind of tumor chemotherapeutic medicinal preparation and preparation method thereof, said preparation is the cell membrane of Apoptosis release
Fragment wraps up chemotherapeutics as carrier, and cell membrane is phospholipid bilayer, and with permeability, its chondritic then passes through cell
The azelon centripetal traction force that is formed of silk for being inside referred to as cytoskeleton is maintained, when the chemotherapeutic in higher concentration
In the case of, chemotherapeutics enters intracellular along concentration gradient, and homogeneous solute is formed in the cell, be upset when cell or
When apoptosis, the Partial Protein broken fiber filaments at cytoskeleton attached cell film position or attachment is lost, centripetal traction force is dashed forward
So disappear so that local cells membrane structure outwards expands, protrudes and wrap up cellular content with vesica under export-oriented pulling force effect
Form is discharged into the extracellular sub- hierarchical structure between cell and molecule, by intracellular chemotherapeutics parcel, release
Diameter contains chemotherapeutics vesica at 0.1~1 μm.But in inducing cell release vesica field, commonly using apoptosis induction
Agent, medicine act on cell or promote Apoptosis using ultraviolet irradiating cell, produce vesica.But inducer of apoptosis and
Introduction of medications foreign substance, collection for later stage purpose product can produce influence.Chemotherapy of tumors medicine is disclosed in the patent
Thing preparation is that, by ultraviolet thing treatment inducing cell release vesica, however, ultraviolet induction apoptosis is in laboratory stage, do not have
There is special equipment apoptosis-induced, and batch processing is restricted.Therefore cell is promoted to discharge the skill of vesica in the prior art
Art has larger limitation.
In summary, it is existing promote cell release vesica technology in actual use, it is clear that exist inconvenience and defect, institute
To be necessary to be improved.
Utility model content
For above-mentioned defect, the purpose of this utility model is to provide a kind of equipment for promoting cell to discharge vesica, with
Special equipment inducing cell apoptosis are provided, make the batch operation of promotion cell release vesica more convenient.
To achieve these goals, the utility model provides a kind of equipment for promoting cell to discharge vesica, the equipment bag
Include：
Cell delivery device, is connected with cell release vesica device, and the cell delivery of the concentration after culture is released to cell
Put in vesica device；
Cell discharges vesica device, including container and irradiation light, and the irradiation light is fixed on the container, and default
Under conditions of the irradiation of the scheduled time is carried out to the cell, make the cell release vesica.
According to described equipment, the cell delivery device includes：
Fluid infusion bottle, the cell of the concentration after the culture is contained in the fluid infusion bottle；
Chemotherapy supplements bottle, and the accommodating chemotherapeutics of chemotherapy supplement bottle is completed to institute in cell release vesica device
After stating the irradiation of cell, the chemotherapeutics is transported in the cell release vesica device；
With peristaltic pump be connected fluid infusion bottle by least two flexible pipes, one of them described flexible pipe；Another described flexible pipe
Fluid infusion bottle is connected with peristaltic pump；
Peristaltic pump, its one end is connected to the flexible pipe, and the other end is connected with cell release vesica device.
According to described equipment, the cell release vesica device also includes：
Container cover, lid is located at the port of the container；At least two passages and fluid infusion are provided with the container cover；
Agitating paddle, the head end on its top is stretched out outside the container cover, and its underpart is provided with mixing part, and stretches into described
In container；
At least two sterilizing filters, sterilizing filter described at least two connects with least described two passages respectively
Connect；
One end of the peristaltic pump is connected with the fluid infusion in cell release vesica device.
According to described equipment, the irradiation light fixed installation in the above-described container, and sets outside the irradiation light
It is equipped with quartz ampoule.
According to described equipment, the agitating paddle is vertically installed in the center of the container；The irradiation light with it is described
Agitating paddle be arranged in parallel.
According to described equipment, the agitating paddle includes being arranged on the rotating handle in portion；It is provided with the rotating handle
Irradiation hole；The irradiation light is arranged on the inside of the rotating handle, and the irradiation light light by the irradiation Kong Zhao
The cell penetrated in the container.
According to described equipment, the mixing part includes multiple blades being uniformly distributed circumferentially, in the blade extremely
Lack and be provided with the irradiation light on a piece of；Or
The mixing part is anchor formula mixing part, and the anchor formula mixing part includes a line of transverse direction and perpendicular to the transverse direction
A line two vertical edges, and be provided with the anchor formula mixing part irradiation hole, in the anchor formula mixing part extremely
The irradiation light is fixed with few two sides, the light of the irradiation light irradiates thin in the container by the irradiation hole
Born of the same parents.
According to described equipment, the cross section of the port of the container is in annular；In the container cover and the container
Port between be provided with sealing ring；
The outside of the container is provided with fixed support, and the fixed support supports the container；
The irradiation light is ultraviolet radiator.
According to described equipment, the material of the container is clear glass；The material of the sealing ring is rubber；
The bottle cap of fluid infusion bottle is two logical bottle caps, and a wherein through holes of two logical bottle caps are connected with flexible pipe, another through hole and
The sterilizing filter connection.
According to described equipment, the equipment also includes：
The lower end of the container cover is arranged on, and arranged in parallel with the agitating paddle, detects temperature in the container
Hygrosensor；And/or
The lower end of the container cover is arranged on, and arranged in parallel with the agitating paddle, detects pH value in the container
PH detectors；And/or
The lower end of the container cover is arranged on, and it is arranged in parallel with the agitating paddle, detect the oxygen content amount in the container
Dissolved oxygen detector；And/or
It is arranged in the container cover, CO is injected to the container2Gas, adjusts the CO of the pH value in the container2Ventilation
Hole.
The utility model is set to include that cell delivery device and cell are released by by the equipment for promoting cell to discharge vesica
Vesica device is put, wherein cell delivery device is connected with cell release vesica device, the cell delivery of the concentration after culture is arrived
In cell release vesica device；After the cell of concentration of the container of cell release vesica device after accommodating culture is defeated, cell is released
Put the irradiation light of vesica device carries out the irradiation of the scheduled time under the conditions of default to the cell, to add again after processing cell
Enter chemotherapeutics, make the cell release vesica.Thus, there is provided special equipment inducing cell apoptosis, release promotion cell
The batch operation for putting vesica is more convenient.
Brief description of the drawings
Fig. 1 is the longitudinal sectional view of the equipment for promoting cell release vesica that the utility model embodiment is provided；
Fig. 2 is the longitudinal sectional view of the equipment for promoting cell release vesica that the utility model embodiment is provided；
Fig. 3 is the top view of the equipment for promoting cell release vesica that the utility model embodiment is provided；
Fig. 4 is the top view of the equipment for promoting cell release vesica that the utility model embodiment is provided；
Fig. 5 is the fixed form of the irradiation light in the equipment for promoting cell release vesica that the utility model embodiment is provided
Schematic diagram；
Fig. 6 is the fixed form of the irradiation light in the equipment for promoting cell release vesica that the utility model embodiment is provided
Schematic diagram；
Fig. 7 is the longitudinal sectional view of the equipment for promoting cell release vesica that the utility model embodiment is provided.
Specific embodiment
In order that the purpose of this utility model, technical scheme and advantage become more apparent, below in conjunction with accompanying drawing and implementation
Example, is further elaborated to the utility model.It should be appreciated that specific embodiment described herein is only used to explain
The utility model, is not used to limit the utility model.
Referring to Fig. 1 and Fig. 2, in first embodiment of the present utility model, there is provided a kind of to promote cell to discharge vesica
Equipment 100, the equipment 100 includes：
Cell delivery device 20, is connected, by the cell delivery of the concentration after culture to carefully with cell release vesica device 10
In born of the same parents' release vesica device 10；
Cell release vesica device 10, including container 11 and irradiation light 14, the irradiation light 14 are fixed on the container
11, and the irradiation of the scheduled time is carried out to the cell under the conditions of default, make the cell release vesica.
In this embodiment, there is provided promote cell to discharge the equipment 100 of vesica, the equipment is by the He of cell delivery device 20
Cell release vesica device 10 is constituted.Wherein, the cell of the concentration after culture is equipped with cell delivery device 20, and this is thin
Born of the same parents' conveying device 20 is connected with cell release vesica device 10.By cell delivery device 20 by the cell of the concentration after culture
After being transported in the container of cell release vesica device 10, cell release vesica device 10 when default condition is reached, will
Starting 14 pairs of cells of irradiation light carries out the irradiation of the scheduled time, makes the cell release vesica.It is described it is pre-conditioned be institute
State the temperature in container 11 and reach 37 DEG C or so, such as 36 DEG C~37 DEG C；And the pH value in the container 11 is 7 or so, therefore
The pH value that the preferred pre-conditioned temperature in the container 11 reaches in 37 DEG C and the container 11 is 7.0.And
The irradiation light 14 is ultraviolet radiator 14；Certainly other kinds of irradiation light 14 is can also be, is such as infrared light.
The cell of the concentration after the culture is tumour cell, it is recommended that from same type of swollen with the tumour cell to be treated
Oncocyte, the cell vesicle can easily with patient's body in tumour cell cell membrane fusion.Such as Application No.
Tumour cell described in the Chinese patent of CN102302784A.In cell cultivation process, cell concentration is 1~10 × 106
The concentration of individual/ml, and with irradiation light 14,1~2 × 10 when such as the ultraviolet induction vesica of ultraviolet radiator 14 discharges7Individual/
The concentration of ml, so cell concentration is lifted in ultraviolet pre-irradiation, by centrifugation or
Quantitative concentration is carried out otherwise, the cell of the concentration after being cultivated.
Referring to Fig. 1 and Fig. 2, in second embodiment of the present utility model, the cell delivery device 20 includes：
Fluid infusion bottle 26, the cell of the concentration after the culture is contained in the fluid infusion bottle 26；
Chemotherapy supplements bottle 29, and the chemotherapy supplement bottle 29 houses chemotherapeutics, complete in cell release vesica device 10
After the irradiation of the paired cell, the chemotherapeutics is transported in the cell release vesica device 10；
With peristaltic pump 28 be connected fluid infusion bottle 26 by least two flexible pipes 27, one of them described flexible pipe 27；Another
With peristaltic pump 28 be connected fluid infusion bottle 26 by the flexible pipe 27；
Peristaltic pump 28, its one end is connected to the flexible pipe 27, and the other end is connected with cell release vesica device 10.
In this embodiment, cell delivery device 20 is with fluid infusion bottle 26 and chemotherapy supplement bottle 29, and fluid infusion bottle 26 and change
Supplement bottle 29 is treated to be connected with peristaltic pump 28 by flexible pipe 27, and peristaltic pump 28 is also connected with cell release vesica device 10.Tool
Body, one end of peristaltic pump 28 is connected to the flexible pipe 27, and the other end is connected with cell release vesica device 10.By compacted
The work of dynamic pump 28, can be by the appearance of the cell delivery of the concentration after the culture in fluid infusion bottle 26 to cell release vesica device 10
Device 11；After the irradiation that irradiation light 14 completes the scheduled time, the time of irradiation is specific according to different environment at 5~60 minutes
The irradiation time of needs can be different, and after such as irradiating 10 minutes, be transported to for the chemotherapeutics of chemotherapy supplement bottle 29 by peristaltic pump 28
In the container 11 of the cell release vesica device 10, promote cell release vesica.
Referring to Fig. 1 and Fig. 2, in 3rd embodiment of the present utility model, the cell release vesica device 10 also includes：
Container cover 16, lid is located at the port of the container 11；At least two passages 17 are provided with the container cover 16
And fluid infusion 22；
Agitating paddle 101, the head end on its top is stretched out outside the container cover 16, and its underpart is stretched into the container 11, and
It is provided with mixing part 1002；
At least two sterilizing filters 18, sterilizing filter 18 described at least two with respectively with least described two ventilations
Hole 17 connects；
The other end of the peristaltic pump 28 is connected with the fluid infusion 22 in cell release vesica device 10.
In this embodiment, the port of the container 11 of cell release vesica device 10 is provided with container cover 16, Er Qie
Be provided with least two passages 17 and fluid infusion 22 in container cover 16, two sterilizing filters 18 respectively with it is at least described two
Passage 17 is connected；The other end of peristaltic pump 28 is connected with the fluid infusion 22 in cell release vesica device 10, is passed through
Can be transported to chemotherapeutics in container 11 by the work of peristaltic pump 28.Main material-feeding port 22 is provided with container cover 16, is led to
Stomata 17 and standby hole 19；Material-feeding port 22 is provided with flexible pipe 27, is connected with fluid infusion bottle 26 by flexible pipe 27.Flexible pipe 27 is arranged on compacted
On dynamic pump 28, the bottle cap of fluid infusion bottle 26 is two logical bottle caps, and the two logical through holes of bottle cap one are connected with flexible pipe 27, another through hole and aseptic mistake
Filter 18 is connected.Agitating paddle 101 includes upper and lower part, and outside the head end propelling container lid 16 on its top, its underpart is provided with
Mixing part 1002, and mixing part 1002 is stretched into container 11.
Referring to Fig. 1 and Fig. 3, in the 4th embodiment of the present utility model, irradiation light 14 is fixedly mounted on the container
In 11, and quartz ampoule 13 is provided with outside the irradiation light 14.And agitating paddle 101 is vertically installed in the center of container 11
Place；Irradiation light 14 be arranged in parallel with agitating paddle 101.
In this embodiment, irradiation light 14 is fixedly mounted on container 11, and the one end of agitating paddle 101 is arranged on the container cover 16
Upper to be used for liquid agitation in container 11 is uniform, irradiation light 14 (uviol lamp) is separately installed in container 11, as shown in Figure 1.And
Agitating paddle 101 is preferably vertically installed in the center of container 11, is easy to the liquid agitation in container 11；Additionally, irradiation light
14 are be arranged in parallel with agitating paddle 101.
Referring to Fig. 2, Fig. 4 and Fig. 7, in the 5th embodiment of the present utility model, agitating paddle 101 includes being arranged at it
The rotating handle 1001 on top；Irradiation hole is provided with the rotating handle 1001；Irradiation light 14 is arranged on the rotating handle 1001
Inside, and the irradiation light 14 the cell that is irradiated in the container 11 by the irradiation hole of light.
In this embodiment, irradiation light 14 is fixed on agitating paddle 101 (as shown in Fig. 2 irradiation light 14 not shown in the figure)
For the liquid in exposure cage 11 so as to promote Apoptosis, rotatable portion of the irradiation light 14 (uviol lamp) in agitating paddle 101
The fixed position divided can be that irradiation light 14 is fixed on the rotating handle 1001 of agitating paddle 101；Preferably, uviol lamp 10 is arranged on
The inside of rotating handle 1001 of agitating paddle 9 simultaneously passes through the liquid in the hole exposure cage 11 on rotating handle 1001.
Referring to Fig. 2 and Fig. 5, in the 6th embodiment of the present utility model, the mixing part 1002 of agitating paddle 101 is including more
The irradiation light 14 is provided with the individual blade being uniformly distributed circumferentially, at least a piece of in the blade；Or such as Fig. 6 institutes
Show, mixing part 1002 is anchor formula mixing part 1002, anchor formula mixing part 1002 includes a line of transverse direction and perpendicular to the transverse direction
A line two vertical edges, and be provided with the anchor formula mixing part 1002 irradiation hole, in the anchor formula mixing part
The irradiation light 14 is fixed with 1002 at least two sides, the light of the irradiation light 14 irradiates described by the irradiation hole
Cell in container 11.
In this embodiment, irradiation light 14 is fixed on the mixing part 1002 of agitating paddle 101；Preferably, agitating paddle 101
Irradiation is provided with 1002 points of the mixing part blade being uniformly distributed circumferentially for more blades, at least a piece of in the more blades
Lamp 14 (as shown in Figure 5).1002 points of the mixing part that can also be agitating paddle 101 is anchor formula, at least two on three sides of anchor formula
Irradiation light 14 (uviol lamp) is fixed with bar side；It is further preferred that uviol lamp 14 is arranged on the inside on the side of anchor formula and passes through
Liquid (as shown in Figure 6) in hole exposure cage on side.And it is preferred, the ultraviolet promotees Apoptosis equipment to be included being used for
The support 2 of container 11 is supported, in case container 11 is because external force is inclined or is toppled over；Container 11 is clear glass tank or translucent glass
Tank；The container cover 16 is made up of stainless steel；Sealing ring 15 is provided between container cover 16 and container 11, to ensure container
It is tightly connected with container cover 16, to prevent outside air, bacterium, floating dust etc. from entering into container.The material of container 11 is transparent
Glass or translucent glass, are easy to operator to observe the situation of the object in container, and the material of sealing ring 15 is rubber.More enter
One step, the utility model sets conducting ring and brush structure between the rotatable portion and container cover 16 of agitating paddle 101, in order to
For uviol lamp is powered.
In the embodiment of the utility model the 7th, promoting the equipment 100 of cell release vesica also includes：
The lower end of the container cover 16 is arranged on, and it is arranged in parallel with the agitating paddle 101, detect the temperature in the container
The hygrosensor 21 of degree；And/or
The lower end of the container cover 16 is arranged on, and it is arranged in parallel with the agitating paddle 101, detect the pH in the container
The pH detectors 23 of value；And/or
The lower end of the container cover 16 is arranged on, and it is arranged in parallel with the agitating paddle 101, detect the appearance in the container
The dissolved oxygen detector 25 of oxygen amount；And/or
It is arranged in the container cover 16, CO is injected to the container 112Gas, adjusts pH value in the container 11
CO2Passage 24.
In this embodiment, promoting the equipment 100 of cell release vesica can include hygrosensor 21, pH detectors
23rd, one or a combination set of dissolved oxygen detector 25.Measured respectively by hygrosensor 21, pH detectors 23, dissolved oxygen detector 25
Temperature, pH value and oxygen content amount in container 11, to adjust the numerical value of the above-mentioned multiple parameters in container 11, to adapt to promote carefully
Born of the same parents discharge vesica.
In the eighth embodiment of the present invention, there is provided a kind of described equipment provided using above-mentioned multiple embodiments
100 methods for promoting cell release vesica, methods described includes：
Cell delivery device 20 is by the cell delivery of the concentration after culture to cell release vesica device 10；The step is
The step of conveying；
Irradiation light 14 in cell release vesica device 10 carries out the scheduled time under the conditions of default to the cell
Irradiation, makes the cell release vesica；The step of step is for release.
In this embodiment, the method for promoting cell release vesica is by the concentration after culture by cell delivery device 20
Cell delivery in cell release vesica device 10；Then reach it is pre-conditioned under when, 14 pairs of cells of irradiation light enter
The irradiation of the row scheduled time, finally causes the cell release vesica.
In the 9th embodiment of the invention, the step of the conveying before include：
The step of culture medium is conveyed, adds under predetermined sterilising conditions in the supplement bottle of the cell delivery device 20
Culture medium, and the culture medium is pumped into by the cell by the peristaltic pump 28 of the cell delivery device 20 discharge vesica device
In 10 container 11；
First whipping step, the temperature detected in the container 11 in the hygrosensor 21 of the equipment is 37 DEG C
When, the agitating paddle 101 of the cell release vesica device 10 is opened, and the molten of the equipment was as a child opened predetermined 12~24
Oxygen probe 25, it is 100% to adjust the dissolved oxygen in the container 11, and opens the setting pH value of pH detectors 23 of the equipment
It is 7.0, and by the CO of the equipment2Passage 24 supplements CO to the container 112Adjust the pH value in the container 11；
The step of conveying, includes：Under predetermined sterilising conditions, the cell of the concentration is placed on the supplement bottle
In 26, and the cell pump 28 of the concentration is entered in the container 11 by the peristaltic pump 25；
Include the step of the release：
The irradiation light 14, irradiation 5~60 are opened behind after the cell of the concentration is pumped into the container 5~60 minutes
The irradiation light 14 is closed after minute；
Under predetermined sterilising conditions by chemotherapeutics in the chemotherapeutics of cell delivery device 20 supplement bottle 29,
The culture medium is pumped into container 11 by the peristaltic pump 28；
Opening the agitating paddle 101 makes the chemotherapeutics fully mix, and cell is incubated altogether with chemotherapeutics, promotes cell
Release vesica.
In an embodiment of the invention, the equipment of uv induction apoptosis is the equipment for promoting cell to discharge vesica
100 include container 11, container fixed support 2, quartz ampoule 13, uviol lamp 14, sealing ring 15, container cover 16, passage 17, aseptic
Filter 18, standby hole 19, agitating paddle 101, hygrosensor 21, fluid infusion 22, pH detectors 23, CO2Passage 24, dissolved oxygen
Detector 25, fluid infusion bottle 26, flexible pipe 27, peristaltic pump 28, chemotherapeutics supplement bottle 29, the mixing part 1002 of agitating paddle 101.This
Outward, the container 11 is heated by a heating mantle being socketed in outside the container 11.The heating mantle can be to holding after being powered
Device 11 is heated.The cellular processes of the uv induction apoptosis that the equipment 100 for discharging vesica by the promotion cell is realized,
With efficiency high, the advantage for being easy to operate, can be used for industrialized production.It is specific to promote cell release capsule in 9th embodiment
The step of equipment 100 of bubble promotes cell to discharge the method for vesica includes：
1) it is each connecting line of equipment and cleaning container is clean；
2) using two-point method (pH 4.0 and pH 9.0) calibration pH detectors, slope value is 1.0 ± 0.2；
3) pH detectors are rinsed well, is connected according to Fig. 1 connected modes；
4) moist heat sterilization, 121 DEG C, 30 minutes；
5) sterilizing is finished, and is taken out after temperature is down to room temperature, and solution supplement bottle 26 is added into culture medium in gnotobasis,
Culture medium is pumped into container 11 by peristaltic pump 28；1)~the step of 5) conveyed for the culture medium；
6) opening temperature detector 21, regulation temperature is 37 DEG C, opens agitating paddle 1001, sets mixing speed 10~100
Rev/min；
7) dissolved oxygen probes 25 are opened after 12-24 hours, regulation dissolved oxygen is 100%, that is, set oxygen dissolving value under now state
It is 100%,；PH detectors 23 are opened, pH 7.0, because container 11 is connected by sterilizing filter with air, container 11 is set
Interior liquid pH can be higher than 7.0, now, can start pH control devices, and CO is supplemented through sterilizing filter 18 in container 112To adjust
pH；6)~7) it is the first whipping step；
8) in gnotobasis, the cell of concentration is placed in solution supplement bottle, the cell that will be concentrated by peristaltic pump 28
Pump into container 11；8) for conveying the step of；
9) ultraviolet illuminator 14 is opened after 5~60 minutes after replenishing the cell of concentration, is irradiated 5~60 minutes；
10) uviol lamp is closed, in gnotobasis by chemotherapeutics in chemotherapeutics supplement bottle, by peristaltic pump 28
Culture medium is pumped into container 11；
11) stirring makes chemotherapeutics fully mix, and cell is incubated altogether with chemotherapeutics.9)~11) for release the step of.
In sum, the utility model is set to include cell delivery device by by the equipment for promoting cell to discharge vesica
Vesica device is discharged with cell, wherein cell delivery device is connected with cell release vesica device, by the thin of the concentration after culture
Born of the same parents are transported in cell release vesica device；The cell of concentration of the container of cell release vesica device after accommodating culture is defeated
Afterwards, the irradiation light of cell release vesica device carries out the irradiation of the scheduled time under the conditions of default to the cell, to process
Chemotherapeutics is added after cell, makes the cell release vesica.Thus, there is provided special equipment inducing cell apoptosis, make
Promote the batch operation of cell release vesica more convenient.
Certainly, the utility model can also have other various embodiments, spiritual and its essence without departing substantially from the utility model
In the case of, those of ordinary skill in the art work as can make various corresponding changes and deformation, but these according to the utility model
Corresponding change and deformation should all belong to the scope of the claims appended by the utility model.
Claims (10)
1. the equipment that a kind of promotion cell discharges vesica, it is characterised in that the equipment includes：
Cell delivery device, is connected with cell release vesica device, and the cell delivery of the concentration after culture to cell is discharged into capsule
In bulb apparatus；
Cell discharges vesica device, including container and irradiation light, and the irradiation light is fixed on the container, and in default bar
The irradiation of the scheduled time is carried out under part to the cell, makes the cell release vesica.
2. equipment according to claim 1, it is characterised in that the cell delivery device includes：
Fluid infusion bottle, the cell of the concentration after the culture is contained in the fluid infusion bottle；
Chemotherapy supplements bottle, and the accommodating chemotherapeutics of chemotherapy supplement bottle is completed to described thin in cell release vesica device
After the irradiation of born of the same parents, the chemotherapeutics is transported in the cell release vesica device；
With peristaltic pump be connected fluid infusion bottle by least two flexible pipes, one of them described flexible pipe；Another described flexible pipe is by institute
Fluid infusion bottle is stated to be connected with peristaltic pump；
Peristaltic pump, its one end is connected to the flexible pipe, and the other end is connected with cell release vesica device.
3. equipment according to claim 2, it is characterised in that the cell release vesica device also includes：
Container cover, lid is located at the port of the container；At least two passages and fluid infusion are provided with the container cover；
Agitating paddle, the head end on its top is stretched out outside the container cover, and its underpart is provided with mixing part, and stretches into the container
It is interior；
At least two sterilizing filters, sterilizing filter described at least two is connected with least described two passages respectively；
One end of the peristaltic pump is connected with the fluid infusion in cell release vesica device.
4. equipment according to claim 3, it is characterised in that the irradiation light fixed installation in the above-described container, and
Quartz ampoule is provided with outside the irradiation light.
5. equipment according to claim 4, it is characterised in that the agitating paddle is vertically installed in the center of the container
Place；The irradiation light be arranged in parallel with the agitating paddle.
6. equipment according to claim 3, it is characterised in that the agitating paddle includes being arranged on the rotating handle in portion；
Irradiation hole is provided with the rotating handle；The irradiation light is arranged on the inside of the rotating handle, and the irradiation light light
The cell that line is irradiated in the container by the irradiation hole.
7. equipment according to claim 3, it is characterised in that the mixing part includes multiple leaves being uniformly distributed circumferentially
The irradiation light is provided with piece, at least a piece of in the blade；Or
The mixing part is anchor formula mixing part, and the anchor formula mixing part includes horizontal a line and perpendicular to described horizontal one
Two vertical edges on bar side, and irradiation hole is provided with the anchor formula mixing part, at least the two of the anchor formula mixing part
The irradiation light is fixed with bar side, the light of the irradiation light is by the cell in the irradiation hole irradiation container.
8. equipment according to claim 3, it is characterised in that the cross section of the port of the container is in annular；Institute
Sealing ring is provided between the port for stating container cover and the container；
The outside of the container is provided with fixed support, and the fixed support supports the container；
The irradiation light is ultraviolet radiator.
9. equipment according to claim 8, it is characterised in that the material of the container is clear glass；The sealing ring
Material be rubber；
The bottle cap of fluid infusion bottle is two logical bottle caps, and a wherein through holes of two logical bottle caps are connected with flexible pipe, another through hole with it is described
Sterilizing filter is connected.
10. equipment according to claim 4, it is characterised in that the equipment also includes：
The lower end of the container cover is arranged on, and it is arranged in parallel with the agitating paddle, detect the temperature of the temperature in the container
Detector；And/or
The lower end of the container cover is arranged on, and it is arranged in parallel with the agitating paddle, detect that the pH of the pH value in the container is visited
Survey device；And/or
The lower end of the container cover is arranged on, and arranged in parallel with the agitating paddle, detects the molten of oxygen content amount in the container
Oxygen detector；And/or
It is arranged in the container cover, CO is injected to the container2Gas, adjusts the CO of the pH value in the container2Passage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621145718.0U CN206308369U (en) | 2016-10-20 | 2016-10-20 | The equipment for promoting cell to discharge vesica |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621145718.0U CN206308369U (en) | 2016-10-20 | 2016-10-20 | The equipment for promoting cell to discharge vesica |
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| Publication Number | Publication Date |
|---|---|
| CN206308369U true CN206308369U (en) | 2017-07-07 |
Family
ID=59243317
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621145718.0U Active CN206308369U (en) | 2016-10-20 | 2016-10-20 | The equipment for promoting cell to discharge vesica |
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| CN (1) | CN206308369U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964509A (en) * | 2016-10-20 | 2018-04-27 | 湖北盛齐安生物科技股份有限公司 | Promote the equipment of cell release vesica and its promote the method for cell release vesica |
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2016
- 2016-10-20 CN CN201621145718.0U patent/CN206308369U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964509A (en) * | 2016-10-20 | 2018-04-27 | 湖北盛齐安生物科技股份有限公司 | Promote the equipment of cell release vesica and its promote the method for cell release vesica |
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