CN107964509A - Promote the equipment of cell release vesica and its promote the method for cell release vesica - Google Patents
Promote the equipment of cell release vesica and its promote the method for cell release vesica Download PDFInfo
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- CN107964509A CN107964509A CN201610914751.3A CN201610914751A CN107964509A CN 107964509 A CN107964509 A CN 107964509A CN 201610914751 A CN201610914751 A CN 201610914751A CN 107964509 A CN107964509 A CN 107964509A
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000001737 promoting effect Effects 0.000 claims abstract description 10
- 210000004027 cell Anatomy 0.000 claims description 190
- 239000012530 fluid Substances 0.000 claims description 29
- 238000001802 infusion Methods 0.000 claims description 29
- 230000002572 peristaltic effect Effects 0.000 claims description 28
- 239000013589 supplement Substances 0.000 claims description 21
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 20
- 239000001301 oxygen Substances 0.000 claims description 20
- 229910052760 oxygen Inorganic materials 0.000 claims description 20
- 230000001954 sterilising effect Effects 0.000 claims description 19
- 239000001963 growth medium Substances 0.000 claims description 11
- 238000002512 chemotherapy Methods 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 7
- 239000011521 glass Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 210000004881 tumor cell Anatomy 0.000 claims description 6
- 239000002775 capsule Substances 0.000 claims description 5
- 239000003708 ampul Substances 0.000 claims description 4
- 239000010453 quartz Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000007789 gas Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000000523 sample Substances 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 2
- 230000006907 apoptotic process Effects 0.000 abstract description 15
- 230000001939 inductive effect Effects 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 3
- 230000000973 chemotherapeutic effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 210000004292 cytoskeleton Anatomy 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000033077 cellular process Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002483 medication Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000034217 membrane fusion Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 150000003904 phospholipids Chemical group 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
Abstract
The present invention is suitable for cell technology field, there is provided a kind of equipment for promoting cell release vesica and its method for promoting cell release vesica, the equipment include：Cell delivery device, is connected with cell release vesica device, by the cell delivery of the concentration after culture to cell release vesica device；Cell discharges vesica device, including container and irradiation light, and the irradiation light is fixed on the container, and carries out the irradiation of the scheduled time to the cell under the conditions of default, makes the cell release vesica.Whereby, the present invention provides special equipment inducing cell apoptosis to realize, and makes the batch operation of promotion cell release vesica more convenient.
Description
Technical field
The present invention relates to cell processing techniques field, more particularly to a kind of equipment for promoting cell release vesica and its promotion
The method that cell discharges vesica.
Background technology
A kind of tumor chemotherapeutic medicinal preparation and its preparation side are disclosed in the Chinese patent of Application No. CN102302784A
Method, said preparation are that the cell membrane fragments of Apoptosis release wrap up chemotherapeutics as carrier, and cell membrane is phospholipid bilayer,
With permeability, centripetal traction force that its chondritic is then formed by being referred to as the azelon silk of cytoskeleton into the cell
It is maintained, in the case of the chemotherapeutic in higher concentration, chemotherapeutics enters into the cell along concentration gradient, in the cell shape
Into homogeneous solute, when cell is upset or when apoptosis, the Partial Protein at cytoskeleton attached cell film position is fine
The fracture of dimension silk loses attachment, and centripetal traction force suddenly disappears so that local cells membrane structure is under export-oriented pulling force effect, outwards
Expand, protrude and wrap up cellular content and the extracellular sub-layer between cell and molecule time knot is discharged into form of vesicles
In structure, intracellular chemotherapeutics is wrapped up, release diameter contains chemotherapeutics vesica at 0.1~1 μm.But in inducing cell
Discharge vesica field, commonly using inducer of apoptosis, drug effect in cell or using ultraviolet irradiating cell to promote cell
Apoptosis, produces vesica.But inducer of apoptosis and introduction of medications foreign substance, the collection for later stage purpose product can produce
Influence.It is by ultraviolet thing processing inducing cell release vesica, however, purple that tumor chemotherapeutic medicinal preparation is disclosed in the patent
Outside line is apoptosis-induced to be in laboratory stage, apoptosis-induced without special equipment, and batch processing is restricted.Therefore existing
There is the technology for promoting cell release vesica in technology to have larger limitation.
In summary, existing promotion cell discharges the technology of vesica in actual use, it is clear that there is inconvenience and defect, institute
To be necessary to be improved.
The content of the invention
For it is above-mentioned the defects of, it is an object of the invention to provide it is a kind of promote cell release vesica equipment and its promotion
The method that cell discharges vesica, to provide special device and method inducing cell apoptosis, makes batch of promotion cell release vesica
Amount operation is more convenient.
To achieve these goals, the present invention provides a kind of equipment for promoting cell release vesica, and the equipment includes：
Cell delivery device, is connected with cell release vesica device, the cell delivery of the concentration after culture is released to cell
Put in vesica device；
Cell discharges vesica device, including container and irradiation light, and the irradiation light is fixed on the container, and default
Under conditions of the irradiation of the scheduled time is carried out to the cell, make cell release vesica.
According to the equipment, the cell delivery device includes：
Fluid infusion bottle, the cell of the concentration after the culture are contained in the fluid infusion bottle；
Chemotherapy supplements bottle, and the chemotherapy supplement bottle houses chemotherapeutics, is completed in cell release vesica device to institute
After the irradiation for stating cell, the chemotherapeutics is transported in the cell release vesica device；
The fluid infusion bottle is connected by least two hoses, one of them described hose with peristaltic pump；Another described hose
The fluid infusion bottle is connected with peristaltic pump；
Peristaltic pump, its one end are connected to the hose, and the other end is connected with cell release vesica device.
According to the equipment, the cell release vesica device further includes：
Container cover, lid are located at the port of the container；At least two ventholes and fluid infusion are provided with the container cover；
Agitating paddle, the head end of its upper part are stretched out outside the container cover, and its underpart is provided with mixing part, and are stretched into described
In container；
At least two sterilizing filters, at least two sterilizing filters connect with least described two ventholes respectively
Connect；
One end of the peristaltic pump discharges the fluid infusion in vesica device with the cell and is connected.
According to the equipment, the irradiation light is fixedly mounted in the above-described container, and is set outside the irradiation light
It is equipped with quartz ampoule.
According to the equipment, the agitating paddle is vertically installed at the center of the container；The irradiation light with it is described
Agitating paddle is arranged in parallel.
According to the equipment, the agitating paddle includes the rotating handle for being arranged on portion；It is provided with the rotating handle
Irradiate hole；The irradiation light is arranged on the inside of the rotating handle, and the light of the irradiation light passes through the irradiation Kong Zhao
Penetrate the cell in the container.
According to the equipment, the mixing part includes multiple blades being uniformly distributed circumferentially, in the blade extremely
A few on piece is provided with the irradiation light；Or
The mixing part is anchor formula mixing part, and the anchor formula mixing part includes horizontal a line and perpendicular to the transverse direction
A line two vertical edges, and be provided with the anchor formula mixing part irradiation hole, in the anchor formula mixing part extremely
Be fixed with the irradiation light on few two sides, the light of the irradiation light pass through the irradiation hole irradiate it is thin in the container
Born of the same parents.
According to the equipment, the cross section of the port of the container is in circular ring shape；In the container cover and the container
Port between be provided with sealing ring；
The outside of the container is provided with fixing bracket, and the fixing bracket supports the container；
The irradiation light is ultraviolet radiator；
The preset condition is that the pH value that the temperature in the container reaches in 37 DEG C and the container is 7.0；
The scheduled time is 5~60 minutes；
The cell of concentration after the culture is tumour cell.
According to the equipment, the material of the container is transparent glass；The material of the sealing ring is rubber；
The bottle cap of the fluid infusion bottle is two logical bottle caps, and a wherein through holes for two logical bottle caps are connected with hose, another through hole and
The sterilizing filter connection.
According to the equipment, the agitating paddle is vertically installed at the center of the container；The irradiation light with it is described
Agitating paddle is arranged in parallel；And/or
The equipment further includes：
Hygrosensor, is arranged on the lower end of the container cover, and arranged in parallel with the agitating paddle；The temperature sensing
Device detects the temperature in the container；And/or
PH detectors, are arranged on the lower end of the container cover, and arranged in parallel with the agitating paddle；The pH detectors inspection
Survey the pH value in the container；And/or
Dissolved oxygen detector, is arranged on the lower end of the container cover, and arranged in parallel with the agitating paddle；The dissolved oxygen detection
Device detects the oxygen content amount in the container；And/or
CO2Venthole, is arranged in the container cover, passes through the CO2Venthole injects CO to the container2Gas, is adjusted
Save the pH value in the container.
In order to realize another goal of the invention of the present invention, setting described in any of the above-described is used present invention also offers a kind of
The standby method for promoting cell release vesica, the described method includes：
The step of conveying, cell delivery device is by the cell delivery of the concentration after culture to cell release vesica device；
The step of release, the irradiation light that cell is discharged in vesica device make a reservation for the cell under the conditions of default
The irradiation of time, makes the cell release vesica.
The present invention is by the way that the equipment for promoting cell to discharge vesica to be arranged to include cell delivery device and cell release capsule
Bulb apparatus, wherein cell delivery device are connected with cell release vesica device, by the cell delivery of the concentration after culture to cell
Discharge in vesica device；The container of cell release vesica device is after the cell of the concentration after housing culture is defeated, cell release capsule
The irradiation light of bulb apparatus carries out the cell under the conditions of default the irradiation of the scheduled time, to handle adding after cell
Medicine is treated, makes the cell release vesica.Thus, there is provided special equipment and released using promotion cell provided by the invention
Putting the method for vesica with inducing cell apoptosis, can make the batch operation of promotion cell release vesica more convenient.
Brief description of the drawings
Fig. 1 is the longitudinal sectional view of the equipment of promotion cell release vesica provided in an embodiment of the present invention；
Fig. 2 is the longitudinal sectional view of the equipment of promotion cell release vesica provided in an embodiment of the present invention；
Fig. 3 is the top view of the equipment of promotion cell release vesica provided in an embodiment of the present invention；
Fig. 4 is the top view of the equipment of promotion cell release vesica provided in an embodiment of the present invention；
Fig. 5 is the fixed form signal of the irradiation light in the equipment of promotion cell release vesica provided in an embodiment of the present invention
Figure；
Fig. 6 is the fixed form signal of the irradiation light in the equipment of promotion cell release vesica provided in an embodiment of the present invention
Figure；
Fig. 7 is the longitudinal sectional view of the equipment of promotion cell release vesica provided in an embodiment of the present invention；
Fig. 8 is the method flow diagram of promotion cell release vesica provided in an embodiment of the present invention.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, it is right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Referring to Fig. 1 and Fig. 2, in the first embodiment of the present invention, there is provided a kind of equipment for promoting cell release vesica
100, the equipment 100 includes：
Cell delivery device 20, is connected with cell release vesica device 10, by the cell delivery of the concentration after culture to carefully
Born of the same parents are discharged in vesica device 10；
Cell release vesica device 10, including container 11 and irradiation light 14, the irradiation light 14 are fixed on the container
11, and the irradiation under the conditions of default to the cell progress scheduled time, make the cell release vesica.
In this embodiment, there is provided promote the equipment 100 of cell release vesica, the equipment is by 20 He of cell delivery device
Cell release vesica device 10 is formed.Wherein, the cell for the concentration being equipped with cell delivery device 20 after culture, and this is thin
Born of the same parents' conveying device 20 is connected with cell release vesica device 10.By cell delivery device 20 by the cell of the concentration after culture
After being transported in the container of cell release vesica device 10, cell release vesica device 10 when reaching default condition, general
Start the irradiation that irradiation light 14 carries out the cell scheduled time, make the cell release vesica.The preset condition is institute
State the temperature in container 11 and reach 37 DEG C or so, such as 36 DEG C~37 DEG C；And the pH value in the container 11 is 7 or so, therefore
The preferable preset condition is that the pH value that the temperature in the container 11 reaches in 37 DEG C and the container 11 is 7.0.And
The irradiation light 14 is ultraviolet radiator 14；Certainly it can also be other kinds of irradiation light 14, be such as infrared light.
The cell of concentration after the culture is tumour cell, it is recommended that from same type of swollen with the tumour cell to be treated
Oncocyte, the cell membrane fusion that the cell vesicle can easily with the tumour cell of patient's body.For example, application number
Tumour cell described in Chinese patent for CN102302784A.In cell cultivation process, cell concentration 1~10 ×
106The concentration of a/ml, and with irradiation light 14, as ultraviolet radiator 14 the release of ultraviolet induction vesica when 1~2 × 107
The concentration of a/ml, so cell concentration is lifted in ultraviolet pre-irradiation, by centrifuging or carrying out otherwise in quantity
Concentration, the cell of the concentration after being cultivated.
Referring to Fig. 1 and Fig. 2, in the second embodiment of the present invention, the cell delivery device 20 includes：
Fluid infusion bottle 26, the cell of the concentration after the culture are contained in the fluid infusion bottle 26；
Chemotherapy supplements bottle 29, and the chemotherapy supplement bottle 29 houses chemotherapeutics, complete in cell release vesica device 10
After the irradiation of the paired cell, the chemotherapeutics is transported in the cell release vesica device 10；
The fluid infusion bottle 26 is connected by least two hoses 27, one of them described hose 27 with peristaltic pump 28；Another
The fluid infusion bottle 26 is connected by the hose 27 with peristaltic pump 28；
Peristaltic pump 28, its one end are connected to the hose 27, and the other end is connected with cell release vesica device 10.
In this embodiment, cell delivery device 20 have fluid infusion bottle 26 and chemotherapy supplement bottle 29, and fluid infusion bottle 26 and change
Supplement bottle 29 is treated to be connected with peristaltic pump 28 by hose 27, and peristaltic pump 28 is also connected with cell release vesica device 10.Tool
Body, one end of peristaltic pump 28 is connected to the hose 27, and the other end is connected with cell release vesica device 10.By compacted
The cell delivery of concentration after culture in fluid infusion bottle 26 to cell, can be discharged the appearance of vesica device 10 by the work of dynamic pump 28
Device 11；After the irradiation that irradiation light 14 completes the scheduled time, the time of irradiation is specific according to different environment at 5~60 minutes
The irradiation time needed can be different, and such as after ten minutes, the chemotherapeutics of chemotherapy supplement bottle 29 is transported to by peristaltic pump 28 for irradiation
In the container 11 of the cell release vesica device 10, promote cell release vesica.
Referring to Fig. 1 and Fig. 2, in the third embodiment of the present invention, the cell release vesica device 10 further includes：
Container cover 16, lid are located at the port of the container 11；At least two ventholes 17 are provided with the container cover 16
And fluid infusion 22；
Agitating paddle 101, the head end of its upper part are stretched out outside the container cover 16, and its underpart is provided with mixing part 1002, and
And stretch into the container 11；
At least two sterilizing filters 18, at least two sterilizing filters 18 with respectively with least described two ventilations
Hole 17 connects；
The other end of the peristaltic pump 28 is connected with the fluid infusion 22 in cell release vesica device 10.
In this embodiment, the port of the container 11 of cell release vesica device 10 is provided with container cover 16, Er Qie
Be provided with least two ventholes 17 and fluid infusion 22 in container cover 16, two sterilizing filters 18 respectively with it is at least described two
Venthole 17 connects；The other end of peristaltic pump 28 is connected with the fluid infusion 22 in cell release vesica device 10, is passed through
Chemotherapeutics can be transported in container 11 by the work of peristaltic pump 28.Main material-feeding port 22 is provided with container cover 16, is led to
Stomata 17 and spare hole 19；Material-feeding port 22 is provided with hose 27, is connected by hose 27 with fluid infusion bottle 26.Hose 27 is arranged on compacted
On dynamic pump 28, the bottle cap of fluid infusion bottle 26 is two logical bottle caps, and two logical one through holes of bottle cap are connected with hose 27, another through hole and sterile mistake
Filter 18 connects.Agitating paddle 101 includes upper and lower part, and outside the head end propelling container lid 16 of its upper part, its underpart is provided with
Mixing part 1002, and mixing part 1002 is stretched into container 11.
Referring to Fig. 1 and Fig. 3, in the 4th embodiment of the present invention, irradiation light 14 is fixedly mounted in the container 11,
And it is provided with quartz ampoule 13 outside the irradiation light 14.And agitating paddle 101 is vertically installed at the center of container 11；Irradiation
Lamp 14 is arranged in parallel with agitating paddle 101.
In this embodiment, irradiation light 14 is fixedly mounted on container 11, and 101 one end of agitating paddle is installed on the container cover 16
Upper to be used for liquid agitation in container 11 is uniform, irradiation light 14 (ultraviolet lamp) is separately installed in container 11, as shown in Figure 1.And
Agitating paddle 101 is preferably vertically installed at the center of container 11, easy to by the liquid agitation in container 11；In addition, irradiation light
14 are arranged in parallel with agitating paddle 101.
Referring to Fig. 2, Fig. 4 and Fig. 7, in the 5th embodiment of the present invention, agitating paddle 101 includes being arranged on portion
Rotating handle 1001；Irradiation hole is provided with the rotating handle 1001；Irradiation light 14 is arranged on the inside of the rotating handle 1001,
And the light of the irradiation light 14 irradiates the cell in the container 11 by the irradiation hole.
In this embodiment, irradiation light 14 is fixed on agitating paddle 101 (as shown in Fig. 2, irradiation light 14 not shown in the figure)
For the liquid in exposure cage 11 so as to promote Apoptosis, rotatable portion of the irradiation light 14 (ultraviolet lamp) in agitating paddle 101
The fixed position divided can be that irradiation light 14 is fixed on the rotating handle 1001 of agitating paddle 101；Preferably, ultraviolet lamp 10 is arranged on
The inside of rotating handle 1001 of agitating paddle 9 simultaneously passes through the liquid in the hole exposure cage 11 on rotating handle 1001.
Referring to Fig. 2 and Fig. 5, in the 6th embodiment of the present invention, the mixing part 1002 of agitating paddle 101 includes multiple edges
Circumferential equally distributed blade, at least on piece in the blade are provided with the irradiation light 14；Or as shown in fig. 6, stir
It is anchor formula mixing part 1002 to mix portion 1002, and anchor formula mixing part 1002 includes horizontal a line and perpendicular to described horizontal one
Two vertical edges on side, and irradiation hole is provided with the anchor formula mixing part 1002, in the anchor formula mixing part 1002
The irradiation light 14 is at least fixed with two sides, the light of the irradiation light 14 irradiates the container 11 by the irradiation hole
Interior cell.
In this embodiment, irradiation light 14 is fixed on the mixing part 1002 of agitating paddle 101；Preferably, agitating paddle 101
The blade being uniformly distributed circumferentially for more blades is divided in mixing part 1002, and at least on piece in the more blades is provided with irradiation
Lamp 14 (as shown in Figure 5).It can also be that the mixing part 1002 of agitating paddle 101 is divided for anchor formula, at least two on three sides of anchor formula
Irradiation light 14 (ultraviolet lamp) is fixed with bar side；It is further preferred that ultraviolet lamp 14 is arranged on the inside on the side of anchor formula and passes through
Liquid (as shown in Figure 6) in hole exposure cage on side.And it is preferable, the ultraviolet, which promotees Apoptosis equipment, to be included being used for
The stent 2 of container 11 is supported, to prevent container 11 because external force tilts or topples over；Container 11 is transparent glass tank or translucent glass
Tank；The container cover 16 is made of stainless steel；Sealing ring 15 is provided between container cover 16 and container 11, to ensure container
It is tightly connected with container cover 16, to prevent outside air, bacterium, floating dust etc. from entering in container.The material of container 11 is transparent
Glass or translucent glass, person easy to operation observes the situation of the object in container, and the material of sealing ring 15 is rubber.More into
One step, the present invention sets conducting ring and brush structure between the rotatable portion and container cover 16 of agitating paddle 101, in order to be purple
Outer lamp power supply.
In seventh embodiment of the invention, the equipment 100 of cell release vesica is promoted to further include：
Hygrosensor 21, is arranged on the lower end of the container cover 16, and arranged in parallel with the agitating paddle 101；It is described
Hygrosensor 21 detects the temperature in the container 11；
PH detectors 23, are arranged on the lower end of the container cover 16, and arranged in parallel with the agitating paddle 101；The pH
Detector 23 detects the pH value in the container 11；
Dissolved oxygen detector 25, is arranged on the lower end of the container cover 16, and arranged in parallel with the agitating paddle 101；It is described
Dissolved oxygen detector detects the oxygen content amount in the container 11；
CO2Venthole 24, is arranged in the container cover 16, passes through the CO2Venthole 24 is injected to the container 11
CO2Gas, adjusts the pH value in the container 11.
In this embodiment, hygrosensor 21, pH detectors can be included by promoting the equipment 100 of cell release vesica
23rd, one or a combination set of dissolved oxygen detector 25.Measured respectively by hygrosensor 21, pH detectors 23, dissolved oxygen detector 25
Temperature, pH value and oxygen content amount in container 11, it is thin to adapt to promote to adjust the numerical value of the above-mentioned multiple parameters in container 11
Born of the same parents discharge vesica.
In the eighth embodiment of the present invention, there is provided a kind of equipment provided using above-mentioned multiple embodiments
100 promote the method for cell release vesica, the described method includes：
In step S801, the cell delivery of the concentration after culture to cell is discharged vesica device 10 by cell delivery device 20
It is interior；The step of step is conveys；
In step S802, the irradiation light 14 in cell release vesica device 10 carries out the cell under the conditions of default
The irradiation of the scheduled time, makes the cell release vesica；The step of step is discharges.
In this embodiment, there is provided promote cell release vesica method by cell delivery device 20 by after culture
The cell delivery of concentration is discharged in vesica device 10 to cell；Then when reaching under preset condition, irradiation light 14 is to described thin
Born of the same parents carry out the irradiation of the scheduled time, finally so that cell release vesica.
In the 9th embodiment of the present invention, include before the step of conveying：
The step of culture medium conveys, adds under predetermined sterilising conditions in the supplement bottle of the cell delivery device 20
Culture medium, and the culture medium is pumped into by the cell release vesica device by the peristaltic pump 28 of the cell delivery device 20
In 10 container 11；
First whipping step, is 37 DEG C in the temperature that the hygrosensor 21 of the equipment is detected in the container 11
When, the agitating paddle 101 of the cell release vesica device 10 is opened, and the molten of the equipment was as a child opened predetermined 12~24
Oxygen probe 25, it is 100% to adjust the dissolved oxygen in the container 11, and opens the setting pH value of pH detectors 23 of the equipment
For 7.0, and pass through the CO of the equipment2Venthole 24 supplements CO to the container 112Adjust the pH value in the container 11；
The step of conveying, includes：Under predetermined sterilising conditions, the cell of the concentration is placed on the supplement bottle
In 26, and the cell pump 28 of the concentration is entered in the container 11 by the peristaltic pump 25；
Include in the step of release：
5 after the cell of the concentration to be pumped into the container~irradiation light 14, irradiation 5~60 are opened after sixty minutes
The irradiation light 14 is closed after minute；
Under predetermined sterilising conditions by chemotherapeutics in 20 chemotherapeutics of cell delivery device supplement bottle 29,
The culture medium is pumped into container 11 by the peristaltic pump 28；
Opening the agitating paddle 101 makes the chemotherapeutics fully mix, and cell is incubated altogether with chemotherapeutics, promotes cell
Discharge vesica.
In an embodiment of the invention, the equipment of uv induction apoptosis is to promote the equipment of cell release vesica
100 include container 11, container fixing bracket 2, quartz ampoule 13, ultraviolet lamp 14, sealing ring 15, container cover 16, venthole 17, sterile
Filter 18, spare hole 19, agitating paddle 101, hygrosensor 21, fluid infusion 22, pH detectors 23, CO2Venthole 24, dissolved oxygen
Detector 25, fluid infusion bottle 26, hose 27, peristaltic pump 28, chemotherapeutics supplement bottle 29, the mixing part 1002 of agitating paddle 101.This
Outside, which is heated by a heating mantle being socketed in outside the container 11.The heating mantle can be to holding after being powered
Device 11 is heated.The cellular processes of the uv induction apoptosis of the realization of equipment 100 of vesica are discharged by the promotion cell,
With advantage efficient, easy to operation, available for industrialized production.It is specific to promote cell release capsule in 9th embodiment
The equipment 100 of bubble promotes the step of method of cell release vesica to include：
1) it is each connecting line of equipment and cleaning container is clean；
2) using two-point method (pH 4.0 and pH 9.0) calibration pH detectors 23, slope value is 1.0 ± 0.2；
3) pH detectors 23 are rinsed well, is connected according to Fig. 1 connection modes；
4) moist heat sterilization, 121 DEG C, 30 minutes；
5) sterilizing finishes, and treats that temperature is cooled to room temperature taking-up, and solution supplement bottle 26 is added culture medium in gnotobasis,
Culture medium is pumped into container 11 by peristaltic pump 28；1) the step of~5) being conveyed for the culture medium；
6) opening temperature detector 21, it is 37 DEG C to adjust temperature, opens agitating paddle 1001, sets mixing speed 10~100
Rev/min；
7) dissolved oxygen probes 25 are opened after when 12-24 is small, it is 100% to adjust dissolved oxygen, that is, sets oxygen dissolving value under state at this time
For 100%,；PH detectors 23 are opened, set pH 7.0, due to container 11 by sterilizing filter with being air connected, container 11
Interior liquid pH can be higher than 7.0, at this time, can start pH control devices, and CO is supplemented through sterilizing filter 18 into container 112To adjust
PH value；6)~7) it is the first whipping step；
8) in gnotobasis, the cell of concentration is placed in solution supplement bottle, by peristaltic pump 28 by the cell of concentration
It is pumped into container 11；8) the step of being the conveying；
9) be added to 5 after the cell of concentration~ultraviolet illuminator 14 is opened after sixty minutes, irradiate 5~60 minutes；
10) ultraviolet lamp is closed, chemotherapeutics is passed through into peristaltic pump 28 in chemotherapeutics supplement bottle in gnotobasis
Culture medium is pumped into container 11；
11) stirring makes chemotherapeutics fully mix, and cell is incubated altogether with chemotherapeutics.9)~11) to discharge the step of.
In conclusion the present invention is arranged to include cell delivery device and carefully by the equipment that cell will be promoted to discharge vesica
Born of the same parents discharge vesica device, and wherein cell delivery device is connected with cell release vesica device, and the cell of the concentration after culture is defeated
It is sent in cell release vesica device；The container of cell release vesica device is after the cell of the concentration after housing culture is defeated, carefully
Born of the same parents discharge irradiation of the irradiation light of vesica device under the conditions of default to the cell progress scheduled time, after handling cell
Chemotherapeutics is added, makes the cell release vesica.Thus, there is provided special equipment inducing cell apoptosis, makes promotion thin
The batch operation that born of the same parents discharge vesica is more convenient.
Certainly, the present invention can also have other various embodiments, ripe in the case of without departing substantially from spirit of the invention and its essence
Know those skilled in the art and make various corresponding changes and deformation in accordance with the present invention, but these change and become accordingly
Shape should all belong to the protection domain of appended claims of the invention.
Claims (10)
1. a kind of equipment for promoting cell release vesica, it is characterised in that the equipment includes：
Cell delivery device, is connected with cell release vesica device, the cell delivery of the concentration after culture to cell is discharged capsule
In bulb apparatus；
Cell discharges vesica device, including container and irradiation light, the irradiation light are fixed on the container, and in default bar
The irradiation of the scheduled time is carried out under part to the cell, makes the cell release vesica.
2. equipment according to claim 1, it is characterised in that the cell delivery device includes：
Fluid infusion bottle, the cell of the concentration after the culture are contained in the fluid infusion bottle；
Chemotherapy supplements bottle, and the chemotherapy supplement bottle houses chemotherapeutics, is completed in cell release vesica device to described thin
After the irradiation of born of the same parents, the chemotherapeutics is transported in the cell release vesica device；
The fluid infusion bottle is connected by least two hoses, one of them described hose with peristaltic pump；Another described hose is by institute
Fluid infusion bottle is stated to be connected with peristaltic pump；
Peristaltic pump, its one end are connected to the hose, and the other end is connected with cell release vesica device.
3. equipment according to claim 2, it is characterised in that the cell release vesica device further includes：
Container cover, lid are located at the port of the container；At least two ventholes and fluid infusion are provided with the container cover；
Agitating paddle, the head end of its upper part are stretched out outside the container cover, and its underpart is provided with mixing part, and stretches into the container
It is interior；
At least two sterilizing filters, at least two sterilizing filters are connected with least described two ventholes respectively；
One end of the peristaltic pump discharges the fluid infusion in vesica device with the cell and is connected.
4. equipment according to claim 3, it is characterised in that the irradiation light is fixedly mounted in the above-described container, and
Quartz ampoule is provided with outside the irradiation light；
The agitating paddle includes the rotating handle for being arranged on portion；Irradiation hole is provided with the rotating handle；The irradiation light is set
Put in the inside of the rotating handle, and the light of the irradiation light irradiates the cell in the container by the irradiation hole.
5. equipment according to claim 3, it is characterised in that the mixing part includes multiple leaves being uniformly distributed circumferentially
Piece, at least on piece in the blade are provided with the irradiation light；Or
The mixing part is anchor formula mixing part, and the anchor formula mixing part includes horizontal a line and perpendicular to described horizontal one
Two vertical edges on bar side, and irradiation hole is provided with the anchor formula mixing part, at least two in the anchor formula mixing part
The irradiation light is fixed with bar side, the light of the irradiation light irradiates the cell in the container by the irradiation hole.
6. equipment according to claim 3, it is characterised in that the cross section of the port of the container is in circular ring shape；Institute
State and be provided with sealing ring between the port of container cover and the container；
The outside of the container is provided with fixing bracket, and the fixing bracket supports the container；
The irradiation light is ultraviolet radiator；
The preset condition is that the pH value that the temperature in the container reaches in 37 DEG C and the container is 7.0；
The scheduled time is 5~60 minutes；
The cell of concentration after the culture is tumour cell.
7. equipment according to claim 6, it is characterised in that the material of the container is transparent glass；The sealing ring
Material be rubber；
The bottle cap of the fluid infusion bottle is two logical bottle caps, and a wherein through holes for two logical bottle caps are connected with hose, another through hole with it is described
Sterilizing filter connects.
8. equipment according to claim 4, it is characterised in that the agitating paddle is vertically installed in the center of the container
Place；The irradiation light is arranged in parallel with the agitating paddle；And/or
The equipment further includes：
Hygrosensor, is arranged on the lower end of the container cover, and arranged in parallel with the agitating paddle；The hygrosensor is visited
Survey the temperature in the container；And/or
PH detectors, are arranged on the lower end of the container cover, and arranged in parallel with the agitating paddle；The pH detectors detect institute
State the pH value in container；And/or
Dissolved oxygen detector, is arranged on the lower end of the container cover, and arranged in parallel with the agitating paddle；The dissolved oxygen detector inspection
Survey the oxygen content amount in the container；And/or
CO2Venthole, is arranged in the container cover, passes through the CO2Venthole injects CO to the container2Gas, adjusts institute
State the pH value in container.
9. a kind of usage right requires the method that 1~8 any one of them equipment promotes cell to discharge vesica, it is characterised in that
The described method includes：
The step of conveying, cell delivery device is by the cell delivery of the concentration after culture to cell release vesica device；
The step of release, the irradiation light that cell is discharged in vesica device carry out the scheduled time under the conditions of default to the cell
Irradiation, make cell release vesica.
10. according to the method described in claim 9, it is characterized in that, include before the step of conveying：
The step of culture medium conveys, adds culture under predetermined sterilising conditions in the supplement bottle of the cell delivery device
Base, and the culture medium is pumped into by the container that the cell discharges vesica device by the peristaltic pump of the cell delivery device
In；
First whipping step, when the temperature that the hygrosensor of the equipment is detected in the container is 37 DEG C, opens institute
The agitating paddle of cell release vesica device is stated, and in predetermined 12~24 dissolved oxygen probes for as a child opening the equipment, adjusting
Dissolved oxygen in the container is 100%, and the pH detectors of the unlatching equipment set pH value as 7.0, and is set by described
Standby CO2Venthole supplements CO to the container2Adjust the pH value in the container；
The step of conveying, includes：Under predetermined sterilising conditions, the cell of the concentration is placed in the supplement bottle, and
The cell of the concentration is pumped into the container by the peristaltic pump；
Include in the step of release：
5 after the cell of the concentration to be pumped into the container~open the irradiation light after sixty minutes, irradiation 5~after sixty minutes
Close the irradiation light；
Under predetermined sterilising conditions by chemotherapeutics in cell delivery device chemotherapeutics supplement bottle, by described
The culture medium is pumped into container by peristaltic pump；
Opening the agitating paddle makes the chemotherapeutics fully mix, and cell is incubated altogether with chemotherapeutics, promotes cell release capsule
Bubble.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610914751.3A CN107964509A (en) | 2016-10-20 | 2016-10-20 | Promote the equipment of cell release vesica and its promote the method for cell release vesica |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610914751.3A CN107964509A (en) | 2016-10-20 | 2016-10-20 | Promote the equipment of cell release vesica and its promote the method for cell release vesica |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107964509A true CN107964509A (en) | 2018-04-27 |
Family
ID=61997193
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610914751.3A Withdrawn CN107964509A (en) | 2016-10-20 | 2016-10-20 | Promote the equipment of cell release vesica and its promote the method for cell release vesica |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107964509A (en) |
Cited By (2)
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| CN110317711A (en) * | 2019-07-11 | 2019-10-11 | 湖北盛齐安生物科技股份有限公司 | The equipment induced cell apoptosis and its method for promoting cell to discharge vesica |
| CN110628719A (en) * | 2019-09-23 | 2019-12-31 | 山东省医学科学院基础医学研究所 | Method for inducing cells to rapidly produce vesicles and application thereof |
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