CN110317711A - The equipment induced cell apoptosis and its method for promoting cell to discharge vesica - Google Patents
The equipment induced cell apoptosis and its method for promoting cell to discharge vesica Download PDFInfo
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- CN110317711A CN110317711A CN201910624720.8A CN201910624720A CN110317711A CN 110317711 A CN110317711 A CN 110317711A CN 201910624720 A CN201910624720 A CN 201910624720A CN 110317711 A CN110317711 A CN 110317711A
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- 230000006907 apoptotic process Effects 0.000 title claims abstract description 93
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000001737 promoting effect Effects 0.000 title claims abstract description 19
- 230000002572 peristaltic effect Effects 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 12
- 239000003814 drug Substances 0.000 claims description 9
- 229940079593 drug Drugs 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 230000009514 concussion Effects 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims 1
- 230000006378 damage Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 219
- 210000004881 tumor cell Anatomy 0.000 description 6
- 230000000973 chemotherapeutic effect Effects 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 2
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- 239000000411 inducer Substances 0.000 description 2
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- 230000001105 regulatory effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000008215 water for injection Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002483 medication Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
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- 238000004659 sterilization and disinfection Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
Abstract
The present invention is suitable for cell technology field, provide a kind of equipment induced cell apoptosis and its method for promoting cell release vesica, the equipment includes: cell delivery unit, accommodates pipe with cell and is connected to connection, the cell delivery of concentration to the cell is accommodated and is managed；Cell accommodates pipe, for holding the cell of the concentration；Promote Apoptosis irradiation light, the above and or below of pipe is accommodated positioned at the cell, the cell of the concentration is irradiated, makes the cell release vesica.Whereby, the present invention is induced cell apoptosis with realizing the special equipment of offer, is kept the batch operation for promoting cell release vesica more convenient and is reduced mechanical force damage, increases the collection of purpose product.
Description
Technical field
The present invention relates to cell processing techniques field more particularly to a kind of equipment induced cell apoptosis and its promote cell
Discharge the method for vesica.
Background technique
A kind of tumor chemotherapeutic medicinal preparation and its preparation are disclosed in the Chinese patent of Publication No. (CN102302784A)
Method, said preparation are that the cell membrane fragments in balloon-shaped structure of Apoptosis release wrap up chemotherapeutics, cell membrane as carrier
For phospholipid bilayer, there is permeability, chondritic then passes through the azelon silk institute for being referred to as cytoskeleton into the cell
The centripetal traction force formed is maintained, and in the case of the chemotherapeutic in higher concentration, chemotherapeutics enters along concentration gradient
Into the cell, uniform solute is formed in the cell, when cell is stimulated or when apoptosis, cytoskeleton attached cell film
The part azelon silk at position is broken or loses attachment, and centripetal traction force suddenly disappears, so that local cells membrane structure is outside
To under pulling force effect, outward expansion, it is prominent and wrap up cellular content with form of vesicles be discharged into it is extracellular between cell and
In sub- hierarchical structure between molecule, intracellular chemotherapeutics is wrapped up, release diameter contains chemotherapeutics at 0.1~1 μm
Vesica.However vesica field is discharged in inducing cell, in cell or ultraviolet light is used commonly using inducer of apoptosis, drug effect
Irradiating cell generates vesica to promote Apoptosis.But inducer of apoptosis and introduction of medications foreign substance, for later period mesh
The collection of product can have an impact.Publication No. (CN107964509A), it is entitled to promote setting for cell release vesica
Disclosing tumor chemotherapeutic medicinal preparation in the Chinese patent of method that is standby and its promoting cell release vesica is by ultraviolet light object
Handle inducing cell discharge vesica, however, the equipment needed when apoptosis-induced agitating paddle agitation make cell be suspended in liquid it
In, but stirring can bring mechanical force to damage to cell, and due to the limitation of UV intensity, cell cannot be adequately exposed to ultraviolet
Line causes the collection of purpose product to tail off, therefore the technology of cell release vesica is promoted to have biggish limitation in the prior art
Property.
In conclusion existing induce cell apoptosis so that the equipment of cell release vesica there will naturally be in actual use
Inconvenient and defect, so it is necessary to be improved.
Summary of the invention
For above-mentioned defect, the purpose of the present invention is to provide a kind of equipment induced cell apoptosis and its promote cell
The method for discharging vesica, is induced cell apoptosis with providing special device and method, makes the batch behaviour for promoting cell release vesica
Make more convenient and reduce mechanical force damage, increases the collection of purpose product.
To achieve the goals above, the present invention provides a kind of equipment induced cell apoptosis, and the equipment includes:
Cell delivery unit accommodates pipe with cell and is connected to connection, the cell delivery of concentration to the cell is accommodated and is managed；
Cell accommodates pipe, for holding the cell of the concentration；
Promote Apoptosis irradiation light, the above and or below of pipe accommodated positioned at the cell, to the cell of the concentration into
Row irradiation.
According to the equipment induced cell apoptosis, the cell delivery unit includes:
Cell holding bottle, for storing the cell of the concentration；
First hose, one end of first hose are protruded into the cell holding bottle, and the other end and the cell accommodate
Pipe connection connection；
First peristaltic pump is fixed on first hose, by the work of first peristaltic pump by the concentration
Cell be delivered to the cell by first hose and accommodate pipe.
According to the equipment induced cell apoptosis, the left and right ends that the cell accommodates pipe offer port, right
The port at end is connected to connection with first hose；
The left end of pipe is accommodated by the cell by the cell for the concentration that the rush Apoptosis irradiation light irradiation finishes
The port discharge.
According to the equipment induced cell apoptosis, it is in cube shape that the cell, which accommodates pipe, and adjacent two are flat
Rounding off between face；Or
It is rounding off between two planes wave-shaped and adjacent of longitudinal cross-section that the cell, which accommodates pipe,.
According to the equipment induced cell apoptosis, the cell accommodates the size of the cross section of the left part of pipe
It is gradually reduced to the direction of the port of its left end；The size of the cross section of its right part is to the port of its right end
Direction be gradually reduced；
It constitutes in each face of the left part, rounding off between two adjacent faces；
It constitutes in each face of the right part, rounding off between two adjacent faces；Or
Multiple Apoptosis irradiation lights that promote are respectively arranged at the recess that the cell wave-shaped accommodates pipe
Place.
According to the equipment induced cell apoptosis, the equipment further include:
Cell receiving flask, for storing the thin of the concentration finished by the rush Apoptosis irradiation light irradiation
Born of the same parents；
Chemotherapeutics holding bottle, for storing the chemotherapeutics；
The cell receiving flask is protruded into second hose, one end of second hose, and the other end connects the cell and accommodates
The port of the left end of pipe；
The chemotherapeutics holding bottle is protruded into third hose, one end of the third hose, and the other end and the cell are received
Collect bottle connection；
Second peristaltic pump is fixed on second hose and the third hose；The second wriggling pump work, will be by
The cell for promoting the concentration that Apoptosis irradiation light irradiation finishes accommodates pipe by the cell and is expelled to the cell receipts
Collect bottle；And the chemotherapeutics stored in the chemotherapeutics holding bottle is sucked into the cell receiving flask.
According to the equipment induced cell apoptosis, the equipment further include:
Shaking table is shaken, the bottom of the cell receiving flask is set to；
Level meter is set to the bottom that the cell accommodates pipe, for detecting and showing whether the cell accommodates pipe
It is horizontal；
Can high low adjustment bracket, including two be set to left side first can high low adjustment support rod and two set
Be placed in right side second can high low adjustment support rod, two described first can high low adjustment support rod and two second can
The bottom of the support rod of high low adjustment is respectively arranged with first base and second base, the first base and second base
Equal regulating height；One end that the cell accommodates pipe be connected to two described first can high low adjustment support rod, the other end
Be connected to two described second can high low adjustment support rod；
It is described promote Apoptosis irradiation light one end can move up and down be connected to two described first can high low adjustment branch
Strut, the other end can move up and down be connected to two described second can high low adjustment support rod.
According to the equipment induced cell apoptosis, the rush Apoptosis irradiation light is ultraviolet radiator；
The cell holding bottle upper cover is equipped with the first bottle cap, and first through hole and second logical is offered on first bottle cap
Hole, first hose pass through the first through hole and protrude into the cell holding bottle；The first nothing is set on second through-hole
Bacterium filter；
The cell accommodates pipe and is made of quartz glass；
The cell receiving flask upper cover is equipped with the second bottle cap, opens up on second bottle cap there are two third through-hole, two
The second sterilizing filter is respectively provided on the third through-hole.
In order to realize another goal of the invention of the invention, set the present invention also provides a kind of using described in any of the above embodiments
The standby method for promoting cell release vesica, which comprises
The cell delivery of concentration to cell is accommodated and is managed by supplying step, cell delivery unit；
Cell vesicle release steps reach 0.2 milli in the liquid thickness that the cell accommodates the cell of the concentration of pipe
Meter or more under conditions of, open promote Apoptosis irradiation light, the cell of the concentration is irradiated 5~30 minutes.
According to the method, include: before the supplying step
The cell of the concentration is stored in cell holding bottle under sterile environment by the storing step of the cell of concentration；And
Whether the level meter by being set to the bottom that the cell accommodates pipe detects the cell receiving pipe horizontal；And by can be high
The first base and/or second base of the bracket of low adjustment adjust the level that the cell accommodates pipe.
The cell vesicle release steps further include:
After the rush Apoptosis irradiation light is irradiated 5~30 minutes to the cell of the concentration, described promote carefully is closed
Born of the same parents' apoptosis irradiation light；And under sterile environment, chemotherapeutics is stored in chemotherapeutics holding bottle；It will by the second peristaltic pump
Pipe is accommodated by the cell by the cell for the concentration that the rush Apoptosis irradiation light irradiation finishes and is expelled to the cell
Receiving flask, and will be described in the chemotherapeutics that stored in chemotherapeutics holding bottle sucking by second peristaltic pump
Cell receiving flask；Concussion shaking table is opened, so that the liquid in the cell receiving flask is uniformly mixed, is shone by the rush Apoptosis
The cell for the concentration that shot-light irradiation finishes is incubated for altogether with the chemotherapeutics, promotes the release of drug holding theca bubble.
The cell delivery of concentration to the cell is accommodated by cell delivery unit and is managed by the present invention, the cell of the concentration
For the tumour cell being concentrated after culture, the above and or below that the cell accommodates pipe is provided with rush Apoptosis
Irradiation light, so that the cell of the concentration receives uniform lamp and shines intensity, by promoting Apoptosis irradiation light to the concentration
Cell is irradiated can be with the apoptosis of inducing cell, so that cell be promoted to discharge vesica.It will be by the rush Apoptosis irradiation light
The cell and chemotherapeutics for the concentration that irradiation finishes are pumped into cell receiving flask, describedization by the effect of the second peristaltic pump
It treats drug to be loaded into cell, releases drug holding theca bubble.The equipment and its promote cell release that the present invention induces cell apoptosis as a result,
The method of vesica is induced cell apoptosis with providing special device and method, makes the batch operation for promoting cell to discharge vesica more
For it is convenient and reduce mechanical force damage, increase the collection of purpose product.
Detailed description of the invention
Fig. 1 is the main view of the equipment provided in an embodiment of the present invention induced cell apoptosis；
Fig. 2 is that cell provided in an embodiment of the present invention accommodates pipe and promotees one of the main view of Apoptosis irradiation light；
Fig. 3 is that cell provided in an embodiment of the present invention accommodates the two of pipe and the main view for promoting Apoptosis irradiation light；
Fig. 4 is the flow chart of the method for promotion cell release vesica provided in an embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Referring to FIG. 1 to FIG. 3, in the first embodiment of the present invention, a kind of equipment 100 induced cell apoptosis is provided,
Equipment 100 includes:
Cell delivery unit 10, accommodates pipe 20 with cell and is connected to connection, and the cell delivery of concentration to cell is accommodated pipe 20；
Cell accommodates pipe 20, for holding the cell of the concentration；
Promote Apoptosis irradiation light 30, the above and or below of pipe 20 accommodated positioned at cell, to the cell of the concentration into
Row irradiation.
In this embodiment, the cell delivery of the concentration by culture to cell is accommodated pipe 20 by cell delivery unit 10,
Rush 30 parallel cell of Apoptosis irradiation light receiving pipe 20 is disposed above and/or lower section, so that cell accommodates in pipe 20
The cell of the concentration is by 30 uniform irradiation of rush Apoptosis irradiation light.When the concentration being delivered in cell receiving pipe 20
When the liquid thickness of cell reaches 0.2~0.3 millimeter, open and promote Apoptosis irradiation light 30, irradiate the cell 5 of the concentration~
It 30 minutes, induces cell apoptosis, so that cell discharges vesica.Promoting Apoptosis irradiation light 30 is preferably ultraviolet light irradiation
Lamp；The cell of the concentration is the tumour cell being concentrated by culture, it is recommended that it is thin to be derived from institute's tumour to be treated
The tumour cell of born of the same parents' same type, the cell vesicle can easily be swallowed by the tumour cell of patient's body.Such as it is public
The number of opening is tumour cell described in the Chinese patent of (CN102302784A).In cell cultivation process, cell concentration 1~
10×106The concentration of a/ml, and with the ultraviolet induction vesica of ultraviolet radiator discharge when 1~2 × 107A/ml's is dense
Degree, by being centrifuged or carrying out quantitative concentration otherwise, obtains so cell concentration is promoted before ultraviolet light irradiation
Obtain the cell of the concentration after culture.
Referring to FIG. 1 to FIG. 3, in the second embodiment of the present invention, the cell delivery unit 10 includes:
Cell holding bottle 11, for storing the cell of the concentration；
One end of first hose 12, the first hose 12 is protruded into cell holding bottle 11, and the other end and cell accommodate pipe 20 and connect
Lead to and connects；
First peristaltic pump 13 is fixed on the first hose 12, by the work of the first peristaltic pump 13 by the concentration
Cell is delivered to cell by the first hose 12 and accommodates pipe 20.
In this embodiment, the cell by the concentration of culture is stored in advance in cell storage in the environment of sterilizing
It in bottle 11, is protruded into cell holding bottle 11 using one end of the first hose 12, the first peristaltic pump 13 is clipped in the outer of the first hose 12
Surface, when needing the cell delivery of the concentration to the cell accommodating pipe 20, the first peristaltic pump 13 is started to work, and is squeezed
First hose 12, so that the liquid in cell holding bottle 11 flows to cell and accommodates pipe 20.
Referring to FIG. 1 to FIG. 3, in the third embodiment of the present invention, the left and right ends that cell accommodates pipe 20 offer port,
The port of its right end is connected to connection with the first hose 12；
The institute of the left end of pipe 20 is accommodated by cell by the cell for the concentration that the irradiation of rush Apoptosis irradiation light 30 finishes
State port discharge.
Particularly, it is in cube shape, and rounding off between two adjacent planes that cell, which accommodates pipe 20,；；Or it is thin
It is rounding off between two planes wave-shaped and adjacent of longitudinal cross-section that born of the same parents, which accommodate pipe 20,.Cell is accommodated into pipe 20
Rounding off can reduce dead angle between two adjacent planes.
Preferably, cell accommodate pipe 20 left part cross section the port from size to its left end side
To being gradually reduced；The size of the cross section of its right part is gradually reduced to the direction of the port of its right end.It is similar to
The shape design that the size of the cross section on the top of the body of bottle is gradually reduced to bottleneck.Constitute each of the left part
In a face, rounding off between two adjacent faces；It constitutes in each face of the right part, it is round and smooth between two adjacent faces
Transition；Shape design can be further reduced the dead angle that cell accommodates pipe 20, avoid liquid holdup, and convenient for cleaning.It is more
A Apoptosis irradiation light that promotees is respectively arranged at the recess that the cell wave-shaped accommodates pipe 20, so that cell holds
The cell of the concentration in pipe 20 received is illuminated uniformly.
Referring to FIG. 1 to FIG. 3, in the fourth embodiment of the present invention, equipment 100 further include:
Cell receiving flask 40, for storing the thin of the concentration finished by the irradiation of rush Apoptosis irradiation light 30
Born of the same parents；
Chemotherapeutics holding bottle 50, for storing the chemotherapeutics；
Cell receiving flask 40 is protruded into second hose 60, one end of the second hose 60, and the other end connects cell and accommodates pipe 20
The port of left end；
Chemotherapeutics holding bottle 50, the other end and cell receiving flask 40 are protruded into third hose 70, one end of third hose 70
Connection；
Second peristaltic pump 80 is fixed on the second hose 60 and third hose 70；The work of second peristaltic pump 80 will be promoted thin
The cell that born of the same parents' apoptosis irradiation light 30 irradiates the concentration finished accommodates pipe 20 by cell and is expelled to cell receiving flask 40；And it will
The chemotherapeutics stored in chemotherapeutics holding bottle 50 sucks cell receiving flask 40.
In this embodiment, promote Apoptosis irradiation light 30 to after the cell irradiation of the concentration scheduled time (according to
The irradiation time that different environment specifically needs can be different), such as after ten minutes, the start-up operation of the second peristaltic pump 80 will be shone for irradiation
The cell of the concentration after penetrating is pumped into cell receiving flask 40 and the second peristaltic pump 80 and chemotherapeutics is sucked cell receiving flask
40, it is illuminated after the cell of the concentration be incubated for altogether with the chemotherapeutics so that the cell of the concentration after illuminated
Release drug holding theca bubble.
Referring to Fig. 1, in the fifth embodiment of the present invention, equipment 100 further include:
Shaking table 90 is shaken, the bottom of the cell receiving flask 40 is set to；Shaking shaking table 90 is preferably the adjustable water of revolving speed
Yawing bed, concussion shaking table 90 generates horizontal concussion so that the liquid in cell receiving flask 40 is uniformly mixed.
Level meter 110 is set to the bottom that the cell accommodates pipe 20, for detecting and showing that the cell accommodates pipe
Whether 20 is horizontal；
Can high low adjustment bracket 120, including two be set to left side first can high low adjustment support rod 121 and
Two be set to right side second can high low adjustment support rod 122, first can the support rod 121 and second of high low adjustment can
The support rod 122 of high low adjustment can be telescopic rod, be provided with position limiting structure on telescopic rod, is limited to scheduled height；
Two first can high low adjustment support rod 121 and two second can the bottom of support rod 122 of high low adjustment be respectively set
There are first base 123 and second base 124, first base 123 and the equal regulating height of second base 124；Cell accommodates
One end of pipe 20 be connected to two first can high low adjustment support rod 121, the other end is connected to two second can high low adjustment
Support rod 122, as a result, two first can high low adjustment support rod 121 and two second can high low adjustment support rod
122 common support cells accommodate pipe 20；When level meter 110 detects that cell receiving pipe 20 is not horizontal, by adjusting the first bottom
Seat 123 and the height of second base 124 are finely adjusted, so that the bubble of level meter 110 is in centre；
Promote Apoptosis irradiation light 30 one end can move up and down be connected to two first can high low adjustment support rod
121, the other end can move up and down be connected to two second can high low adjustment support rod 122.It can high low adjustment first
Support rod 121 and second can the support rod 122 of high low adjustment be respectively provided with the side that connect of Apoptosis irradiation light 30 is promoted
Sliding rail is respectively provided with sliding block on sliding rail, and the both ends for promoting Apoptosis irradiation light 30 are connected on the sliding block of two sides, thus in cunning
It is moved up and down in rail, while limiting the position for promoting Apoptosis irradiation light 30 by setting position limiting structure.Or described first
Can high low adjustment support rod 121 and second can the support rod 122 of high low adjustment connect with Apoptosis irradiation light 30 is promoted
Side is provided with multiple mounting holes, and rush Apoptosis irradiation light 30 is installed in the mounting hole realization of different height and is moved down
It is dynamic.It can move up and down due to promoting Apoptosis irradiation light 30, thereby, it is possible to adjust it to accommodate at a distance from pipe 20 with cell, from
And adjust the received UV intensity of cell institute that cell accommodates the concentration in pipe 20.
Preferably,
11 upper cover of cell holding bottle is equipped with the first bottle cap 130, offer on the first bottle cap 130 first through hole 131 with
And second through-hole 132, the first hose 12 pass through first through hole 131 and protrude into cell holding bottle 11；Is arranged on second through-hole 132
One sterilizing filter 140；
The material of cell holding bottle 11 can be transparency silica glass, be also possible to simple glass material and be made；
Cell accommodates pipe 20 and is made of quartz glass；
40 upper cover of cell receiving flask is equipped with the second bottle cap 150, opens up on the second bottle cap 150 there are two third through-hole 151, two
The second sterilizing filter 160 is respectively provided on a third through-hole 151.
Referring to fig. 4, in the sixth embodiment of the present invention, a kind of use equipment 100 described in any of the above embodiments is provided
Promote the method for cell release vesica, which comprises
The cell delivery of concentration to cell is accommodated pipe 20 by step S401, cell delivery unit 10；
Step S402 reaches 0.2 millimeter or more in the liquid thickness that the cell accommodates the cell of the concentration of pipe 20
Under conditions of, it opens and promotees Apoptosis irradiation light 30, the cell of the concentration is irradiated 5~30 minutes.
In the seventh embodiment of the present invention, include: before step S401
The cell of the concentration is stored in cell holding bottle 11 under sterile environment by the storing step of the cell of concentration；
And the level meter 110 by being set to the bottom that the cell accommodates pipe 20 detects the cell whether accommodate pipe 20 horizontal；With
And by can high low adjustment bracket 120 first base 123 and/or second base 124 adjust the cell and accommodate pipe 20
It is horizontal.
Step S402 further include:
After the rush Apoptosis irradiation light 30 is irradiated 5~30 minutes to the cell of the concentration, the rush is closed
Apoptosis irradiation light 30；And under sterile environment, chemotherapeutics is stored in chemotherapeutics holding bottle 50；It is compacted by second
The cell for irradiating the concentration finished by the rush Apoptosis irradiation light 30 is accommodated 20 row of pipe by the cell by dynamic pump 80
Out to the cell receiving flask 40, and the institute that will be stored in the chemotherapeutics holding bottle 50 by second peristaltic pump 80
It states chemotherapeutics and sucks the cell receiving flask 40；Concussion shaking table 90 is opened, so that the liquid in the cell receiving flask 40 is mixed
It closes uniformly, is incubated for, promotees altogether with the chemotherapeutics by the cell for the concentration that the rush Apoptosis irradiation light irradiation finishes
The release steeped into drug holding theca.
The specific implementation process of 7th embodiment:
1) it sterilizes to equipment 100, all parts of equipment 100 is first used into 0.5MNaOH circulation flushing half an hour；So
It is rinsed 20 minutes with purified water afterwards, then with water for injection circulation flushing 5 minutes；Water for injection rinses 5 minutes；In 121 DEG C of temperature
It spends moist heat sterilization 30 minutes lower；Sterilizing finishes, and is cooled to room temperature taking-up to temperature.
2) culture medium is added in gnotobasis in cell holding bottle 11, will be concentrated after culture by first peristaltic pump 13
Cell is pumped into cell and accommodates in pipe 20；Step 2) is step S401；
3) after after the liquid thickness that cell accommodates the cell for the concentration being added in pipe 20 reaches 0.2-0.3 millimeters
Ultraviolet radiator is opened, is irradiated 5~30 minutes；
4) ultraviolet radiator is closed, passes through chemotherapeutics in chemotherapeutics holding bottle 50 in gnotobasis
The cell that chemotherapeutics is pumped into the concentration in cell receiving flask 40 and after will be illuminated by the second peristaltic pump 80 is pumped into carefully
In born of the same parents' receiving flask 40；
5) open concussion shaking table 90, mix well chemotherapeutics, it is illuminated after the concentration cell and chemotherapeutic
Object is incubated for altogether, releases drug holding theca bubble.Step 3)~5) it is step S402.
It is managed in conclusion the present invention is accommodated the cell delivery of concentration to the cell by cell delivery unit, it is described
The cell of concentration is the tumour cell being concentrated after culture, is provided with positioned at the above and or below that the cell accommodates pipe
Promote Apoptosis irradiation light, so that the cell of the concentration receives uniform lamp and shines intensity, by promoting Apoptosis irradiation light pair
The cell of the concentration is irradiated can be with the apoptosis of inducing cell, so that cell be promoted to discharge vesica.It will be by the rush cell
The cell and chemotherapeutics for the concentration that apoptosis irradiation light irradiation finishes are pumped into cell by the effect of the second peristaltic pump and receive
Collect bottle, the chemotherapeutics is loaded into cell, releases drug holding theca bubble.As a result, the equipment that induces cell apoptosis of the present invention and its
The method for promoting cell release vesica, is induced cell apoptosis with providing special device and method, makes to promote cell release vesica
Batch operation it is more convenient and reduce mechanical force damage, increase the collection of purpose product.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention, ripe
It knows those skilled in the art and makes various corresponding changes and modifications, but these corresponding changes and change in accordance with the present invention
Shape all should fall within the scope of protection of the appended claims of the present invention.
Claims (10)
1. a kind of equipment induced cell apoptosis, which is characterized in that the equipment includes:
Cell delivery unit accommodates pipe with cell and is connected to connection, the cell delivery of concentration to the cell is accommodated and is managed；
Cell accommodates pipe, for holding the cell of the concentration；
Promote Apoptosis irradiation light, the above and or below of pipe is accommodated positioned at the cell, the cell of the concentration is shone
It penetrates.
2. the equipment according to claim 1 induced cell apoptosis, which is characterized in that the cell delivery unit includes:
Cell holding bottle, for storing the cell of the concentration；
First hose, one end of first hose are protruded into the cell holding bottle, and the other end and the cell accommodate Guan Lian
Lead to and connects；
First peristaltic pump is fixed on first hose, by the work of first peristaltic pump by the thin of the concentration
Born of the same parents are delivered to the cell by first hose and accommodate pipe.
3. the equipment according to claim 2 induced cell apoptosis, which is characterized in that the cell accommodates the left and right two of pipe
End offers port, and the port of right end is connected to connection with first hose；
The institute of the left end of pipe is accommodated by the cell by the cell for the concentration that the rush Apoptosis irradiation light irradiation finishes
State port discharge.
4. the equipment according to claim 3 induced cell apoptosis, which is characterized in that it is in cube that the cell, which accommodates pipe,
Shape, and rounding off between two adjacent planes；Or
It is rounding off between two planes wave-shaped and adjacent of longitudinal cross-section that the cell, which accommodates pipe,.
5. the equipment according to claim 4 induced cell apoptosis, which is characterized in that accommodated in the cell of cube shape
The size of the cross section of the left part of pipe is gradually reduced to the direction of the port of its left end；The cross section of its right part
Size be gradually reduced to the direction of the port of its right end；
It constitutes in each face of the left part, rounding off between two adjacent faces；
It constitutes in each face of the right part, rounding off between two adjacent faces；Or
Multiple Apoptosis irradiation lights that promote are respectively arranged at the recess that the cell wave-shaped accommodates pipe.
6. the equipment according to claim 3 induced cell apoptosis, which is characterized in that the equipment further include:
Cell receiving flask, for storing the cell of the concentration finished by the rush Apoptosis irradiation light irradiation；
Chemotherapeutics holding bottle, for storing the chemotherapeutics；
The cell receiving flask is protruded into second hose, one end of second hose, and the other end connects the cell and accommodates pipe
The port of left end；
The chemotherapeutics holding bottle, the other end and the cell receiving flask are protruded into third hose, one end of the third hose
Connection；
Second peristaltic pump is fixed on second hose and the third hose；The second wriggling pump work, will be described
The cell for promoting the concentration that Apoptosis irradiation light irradiation finishes accommodates pipe by the cell and is expelled to the cell receiving flask；
And the chemotherapeutics stored in the chemotherapeutics holding bottle is sucked into the cell receiving flask.
7. the equipment according to claim 6 induced cell apoptosis, which is characterized in that the equipment further include:
Shaking table is shaken, the bottom of the cell receiving flask is set to；
Level meter is set to the bottom that the cell accommodates pipe, for detecting and showing that it is whether horizontal that the cell accommodates pipe；
Can high low adjustment bracket, including two be set to left side first can high low adjustment support rod and two be set to
The second of right side can high low adjustment support rod, two described first can high low adjustment support rod and two second can height
The bottom of the support rod of adjusting is respectively arranged with first base and second base, the first base and second base
Adjust height；One end that the cell accommodates pipe be connected to two described first can high low adjustment support rod, other end connection
In two described second can high low adjustment support rod；
It is described promote Apoptosis irradiation light one end can move up and down be connected to two described first can high low adjustment support rod,
The other end can move up and down be connected to two described second can high low adjustment support rod.
8. the equipment according to claim 6 induced cell apoptosis, which is characterized in that
The rush Apoptosis irradiation light is ultraviolet radiator；
The cell holding bottle upper cover is equipped with the first bottle cap, offers first through hole and the second through-hole on first bottle cap,
First hose passes through the first through hole and protrudes into the cell holding bottle；First sterile mistake is set on second through-hole
Filter；
The cell accommodates pipe and is made of quartz glass；
The cell receiving flask upper cover is equipped with the second bottle cap, opens up that there are two third through-holes on second bottle cap, described in two
The second sterilizing filter is respectively provided on third through-hole.
9. a kind of method for promoting cell release vesica using according to any one of claims 1 to 88 described in any item equipment, which is characterized in that
The described method includes:
The cell delivery of concentration to cell is accommodated and is managed by supplying step, cell delivery unit；
Cell vesicle release steps, the cell accommodate pipe the concentration cell liquid thickness reach 0.2 millimeter with
Under conditions of upper, open and promote Apoptosis irradiation light, the cell of the concentration is irradiated 5~30 minutes.
10. according to the method described in claim 9, it is characterized in that,
Include: before the supplying step
The cell of the concentration is stored in cell holding bottle under sterile environment by the storing step of the cell of concentration；And pass through
Whether the level meter detection cell receiving pipe for being set to the bottom that the cell accommodates pipe is horizontal；And by can high low-key
The first base and/or second base of the bracket of section adjust the level that the cell accommodates pipe.
The cell vesicle release steps further include:
After the rush Apoptosis irradiation light is irradiated 5~30 minutes to the cell of the concentration, closes the rush cell and wither
Die irradiation light；And under sterile environment, chemotherapeutics is stored in chemotherapeutics holding bottle；It will be by institute by the second peristaltic pump
The cell for stating the concentration that rush Apoptosis irradiation light irradiation finishes accommodates pipe by the cell and is expelled to the cell collection
Bottle, and the chemotherapeutics stored in the chemotherapeutics holding bottle is sucked by the cell by second peristaltic pump
Receiving flask；Concussion shaking table is opened, so that the liquid in the cell receiving flask is uniformly mixed, by the rush Apoptosis irradiation light
The cell for irradiating the concentration finished is incubated for altogether with the chemotherapeutics, promotes the release of drug holding theca bubble.
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| CN201910624720.8A CN110317711A (en) | 2019-07-11 | 2019-07-11 | The equipment induced cell apoptosis and its method for promoting cell to discharge vesica |
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| CN201910624720.8A CN110317711A (en) | 2019-07-11 | 2019-07-11 | The equipment induced cell apoptosis and its method for promoting cell to discharge vesica |
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