CN205246517U - Container that is fit for use in survey - Google Patents
Container that is fit for use in survey Download PDFInfo
- Publication number
- CN205246517U CN205246517U CN201521128656.8U CN201521128656U CN205246517U CN 205246517 U CN205246517 U CN 205246517U CN 201521128656 U CN201521128656 U CN 201521128656U CN 205246517 U CN205246517 U CN 205246517U
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- Prior art keywords
- chamber
- container
- neck
- room
- opening
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Abstract
The utility model discloses a container that is fit for use in survey, include: the neck has the opening that runs through it, the main cavity room has upper portion room, middle part room and lower part room, with cell individual layer cavity, has the chamber of holding. The upper end of main cavity room is connected with the lower extreme of neck, and the lower extreme and the upper end of cell individual layer cavity of main cavity room are connected, and the inner chamber of opening, main cavity room and hold the chamber intercommunication. The utility model discloses a volume of container is big, can hold more virion, the neck open -ended sets up, helps the prevention possibility that the sample splashes and pollutes under the condition that the container dropped, the upper portion room of main cavity room and the effect that there is the buffering vibration middle part room, the cross -sectional area of the lower part room of main cavity room reduces gradually, and accessible centrifugation realizes virion's part and concentrates, only produce protruding meniscus in the cell individual layer cavity, help using the immunofluorescence detection method to carry out accurate quick required usefulness (monoclonal) the antibody of virus diagnosis and cover the cell accurately.
Description
Technical field
The utility model relates to a kind of container that is adapted at use in mensuration, espespecially a kind of for making the container of the fast quick-recovery of virus that comes from human body or animal specimen.
Background technology
Differentiate that it is to be inoculated in container that viral routine diagnosis process comprises the steps: the selected specific cells to certain viral susceptible, then supposition is inoculated in to this cell culture containing virulent biological sample, subsequently the cell culture of inoculation is cultivated, this cell detects or investigates via viral-induced cytopathic effect for carry out virus by immunofluorescence. In said process, the container of inoculating cell system plays important effect, and its shape, structure etc. have a great impact the tool such as cultivation, viral diagnosis of cell.
Utility model content
The utility model provides a kind of container that is adapted at use in mensuration, and it makes sample be easy to enter in it, and contributes to the possibility of preventing sample to splash, and also helps the Accurate Analysis in diagnostic procedure.
Especially, the utility model provides a kind of container that is adapted at use in mensuration, comprising:
Neck, described neck has the opening that runs through it;
Main chamber, described main chamber comprises:
Upper chambers, the cross-sectional area of its inner chamber increases gradually along direction from top to bottom,
Chamber, middle part, the cross-sectional area of its inner chamber remains unchanged along direction from top to bottom, and
Bottom compartment, the cross-sectional area of its inner chamber reduces gradually along direction from top to bottom;
And
Cell monolayer chamber, described cell monolayer chamber has the container cavity for holding sample,
The upper end of described main chamber is connected with the lower end of described neck, and the lower end of described main chamber is connected with the upper end of described cell monolayer chamber, and the inner chamber of described opening, described main chamber and described container cavity are communicated with.
Alternatively, the shape of the rounded approaching or similar circle of the cross section of the inner chamber of described upper chambers, chamber, described middle part and described bottom compartment.
Alternatively, described bottom compartment can be infundibulate.
Alternatively, the cross-sectional area of the described opening of described neck can be less than the cross-sectional area of the opening of described cell monolayer chamber.
Alternatively, the ratio of the described opening of described neck, the inner chamber of described main chamber and the degree of depth of described container cavity can be 2:10:3.
Alternatively, the described opening of described neck can be circular open, and described container cavity can be circular cylindrical cavity.
Alternatively, the diameter of the described opening of described neck can be 4mm, and the degree of depth can be 2mm.
Alternatively, the height of described main chamber can be 10mm, and the degree of depth of described container cavity can be 3mm.
Alternatively, described wall of a container is thick can be 1mm.
Alternatively, the volume of described cell monolayer chamber can be 28 microlitres.
Alternatively, the volume of described container can be 566 microlitres.
The container that the utility model provides has following beneficial effect:
The volume of this container is large, can hold more virion, is convenient to viral diagnosis;
The opening of neck is less, contribute to the prevention possibility that sample splashes in the situation that container drops pre-antipollution possibility, and opening is easy to sealing;
The effect of buffering vibration is played in the upper chambers of main chamber and chamber, middle part, and the bottom compartment of main chamber realizes in the segment set of virion by centrifugation, is convenient to viral diagnosis;
Cell monolayer chamber reduces or eliminates may the damaging of cell that is grown in cell monolayer cavity bottom, and in cell monolayer chamber, only produce protruding meniscus, ensure that all cells in cell monolayer chamber contacts with appropriate dyestuff-monoclonal antibody, is convenient to the accurate analysis in diagnostic procedure.
By means of accompanying drawing, other feature and advantage of container will be illustrated in description subsequently.
Brief description of the drawings
Embodiment of the present utility model describes in appended non-limitative drawings, wherein:
Fig. 1 is the structural representation according to container of the present utility model; And
Fig. 2 is the sectional structure schematic diagram of the container shown in Fig. 1.
Detailed description of the invention
Embodiment of the present utility model provides a kind of container that makes the fast quick-recovery of virus that comes from human body or animal specimen, and its concrete structure is as follows:
As shown in Figure 1, the container of the present embodiment comprises 3 parts substantially:
(1) neck 2: it has the circular open 1 for filling, the diameter of opening 1 can be 4mm, the degree of depth can be 2mm (, the height of neck 2 can be 2mm).
(2) main chamber 3: the height of main chamber can be 10mm, and main chamber comprises:
Upper chambers 30, can be highly 3mm, and the cross-sectional area of its inner chamber inner chamber area of the sidewall of main chamber (perpendicular to) increases from top to bottom gradually;
Chamber 31, middle part, can be highly 3mm, and the cross-sectional area of its inner chamber remains unchanged from top to bottom, and the diameter of cross section is 10mm;
Bottom compartment 32, is infundibulate, and it can be highly 4mm, and the cross-sectional area of its inner chamber reduces from top to bottom gradually.
(3) cell monolayer chamber 4: tissue is cultivated chamber, it has columniform container cavity, the degree of depth of container cavity can be 3mm, and the diameter of the opening of cell monolayer chamber is greater than the diameter of neck opening, make the cross-sectional area of the opening of cell monolayer chamber be greater than the cross-sectional area of neck opening.
The total measurement (volume) of container can be 566 microlitres, and wall thickness can be 1mm, the volume largest portion that main chamber 3 is container, and the volume of container cavity can be 28 microlitres.
The container of the present embodiment has the following advantages:
(I) volume: its total measurement (volume) can be 566 microlitres, volume is larger, in viral diagnosis process, the volume of container is larger, the sample holding in it is more, and the amount of the sample for filling is depended in the existence of virion in sample, more sample size provides more virion, is conducive to viral diagnosis.
(II) neck opening: it contributes to the possibility of preventing sample to splash has been avoided the possibility of being polluted by other materials simultaneously; Little and outstanding neck opening is suitable for applying plastic bonding film makes neck closure, and the sample in container in the situation that strongly rocking can not spilt from opening.
(III) main chamber: the cross-sectional area of the inner chamber of its upper chambers increases gradually, upper chambers represents " buffering " part of fierce not professional the controlling (knocking) of container opposing, can absorb the vibration from top; Its chamber, middle part can make the vibration of transmitting from upper chambers stop, and buffering vibration from bottom compartment from the vibration of cell monolayer chamber and part; The cross-sectional area of the inner chamber of chamber, its underpart reduces gradually, can realize concentrating of virion by centrifugation, so that the virion after concentrating enters cell monolayer chamber, is convenient to viral diagnosis.
(IV) cell monolayer chamber: reduce or eliminated because cell monolayer chamber top fluid sample is overweight to being grown in may damaging that the cell of cell monolayer cavity bottom causes; And funnel-form bottom compartment extension forms extending space together with the wall of cell monolayer chamber, allow only to produce protruding meniscus in cell monolayer chamber, this protruding meniscus ensures that all cells in cell monolayer chamber contacts with appropriate dyestuff-monoclonal antibody.
Particularly, as shown in Figure 2, container intracavity can be divided into three part A, the B and the B1 that separate with dotted line, and B is similar with B1 part. The flowing velocity and the weight that enter into the fluid sample of the process A part of cell monolayer chamber 4 from neck opening 1 are constant, and the flowing velocity of the flowing velocity of fluid sample in B and B1 part and the fluid sample in A part that varies in weight and weight (less volume produces less speed). In the time that the fluid sample in container bears the gravity producing due to centrifugal force, and in the time that fluid sample arrives bottom compartment, fluid sample does not flow straight down, but turns to certain angle. The fluid sample turning in B and B1 part produces turbulent flow, the fluid sample of turbulent flow disturbs the vertical of fluid sample in A part to flow, and slow down the flowing velocity of fluid sample in A part, make fluid sample in A part under less gravity, arrive the surface of cell monolayer chamber. The similar variation that turbulent flow causes also exists in B and B1 part. Reduce like this or eliminated because cell monolayer chamber top fluid sample is overweight to being grown in may damaging that the cell of cell monolayer cavity bottom causes.
In addition, the volume of cell monolayer chamber (28 microlitre) is designed for the dyestuff that absorbs 25 microlitres especially, and this is enough to cover all cells in cell monolayer chamber, and can the expensive monoclonal antibody of excessive use.
In above-described embodiment of the present utility model, the inner chamber of neck opening, main chamber and the degree of depth of container cavity are respectively 2mm, 10mm and 3mm, but the inner chamber of neck opening, main chamber and the degree of depth of container cavity can, for other values, be 2:10:3 as long as keep three's ratio.
Described container can have any version in shape, size and size, to be suitable for the being used as parts of diagnostic analysis as described in use of the present utility model and field. This area with should be understood that the container that can use many different sizes with the technical staff in the relevant use of sample analysis and the use of diagnostic analysis of diagnosis, particularly, as long as the design of container size and main chamber keep relative ratio.
In the application's a embodiment, this volume of a container is 566 microlitres, and the degree of depth of the opening of neck, the inner chamber of main chamber and container cavity is respectively 2mm, 10mm and 3mm, and this container is designed to be used in standard laboratory microplate, microwell plate or porous plate. In other embodiments, container can be designed in 6,24,96,384,1536,3456 or 9600 orifice plates and the microwell plate of or configuration big or small for any other.
It will be appreciated by those skilled in the art that and can carry out many variations and/or amendment to described embodiment, and do not depart from the wide in range overall range of present disclosure. Therefore, comprise these embodiment that allow the specific dimensions that is suitable for being used in the container in microwell plate, all should be considered in every respect illustrative and not restrictive.
Claims (10)
1. be adapted at the container using in measuring, comprise:
Neck, described neck has the opening that runs through it;
Main chamber, described main chamber comprises:
Upper chambers, the cross-sectional area of its inner chamber increases gradually along direction from top to bottom,
Chamber, middle part, the cross-sectional area of its inner chamber remains unchanged along direction from top to bottom, and
Bottom compartment, the cross-sectional area of its inner chamber reduces gradually along direction from top to bottom;
And
Cell monolayer chamber, described cell monolayer chamber has the container cavity for holding sample,
The upper end of described main chamber is connected with the lower end of described neck, the lower end of described main chamber and described thinThe upper end of born of the same parents' individual layer chamber connects, and the inner chamber of the described opening of described neck, described main chamber and described inContainer cavity is communicated with.
2. container according to claim 1, wherein said upper chambers, chamber, described middle part and described underThe cross section of the inner chamber of portion chamber is rounded.
3. container according to claim 1, wherein said bottom compartment is infundibulate.
4. container according to claim 1, the cross-sectional area of the described opening of wherein said neck is littleIn the cross-sectional area of the opening of described cell monolayer chamber.
5. according to the container described in any one in claim 1-4, the described opening of wherein said neck,The ratio of the inner chamber of described main chamber and the degree of depth of described container cavity is 2:10:3.
6. container according to claim 5, the described opening of wherein said neck is circular open,And described container cavity is circular cylindrical cavity.
7. container according to claim 6, the diameter of the described opening of wherein said neck is 4 millisRice, the degree of depth is 2 millimeters.
8. container according to claim 7, wherein said wall of a container is thick is 1 millimeter.
9. container according to claim 7, the volume of wherein said cell monolayer chamber is 28 microlitres.
10. container according to claim 9, the volume of wherein said container is 566 microlitres.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521128656.8U CN205246517U (en) | 2015-12-29 | 2015-12-29 | Container that is fit for use in survey |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521128656.8U CN205246517U (en) | 2015-12-29 | 2015-12-29 | Container that is fit for use in survey |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205246517U true CN205246517U (en) | 2016-05-18 |
Family
ID=55945590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521128656.8U Expired - Fee Related CN205246517U (en) | 2015-12-29 | 2015-12-29 | Container that is fit for use in survey |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205246517U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317711A (en) * | 2019-07-11 | 2019-10-11 | 湖北盛齐安生物科技股份有限公司 | The equipment induced cell apoptosis and its method for promoting cell to discharge vesica |
-
2015
- 2015-12-29 CN CN201521128656.8U patent/CN205246517U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317711A (en) * | 2019-07-11 | 2019-10-11 | 湖北盛齐安生物科技股份有限公司 | The equipment induced cell apoptosis and its method for promoting cell to discharge vesica |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20161229 Address after: Belize City Belize Patentee after: The Nautilus Foundation Address before: Australian Capital Territory Patentee before: RATCLIFFE PETER |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160518 Termination date: 20211229 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |