CN206219598U - A kind of device for cell spinner bottle culture - Google Patents

A kind of device for cell spinner bottle culture Download PDF

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Publication number
CN206219598U
CN206219598U CN201621220702.1U CN201621220702U CN206219598U CN 206219598 U CN206219598 U CN 206219598U CN 201621220702 U CN201621220702 U CN 201621220702U CN 206219598 U CN206219598 U CN 206219598U
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China
Prior art keywords
suction pipe
bottle
inlet
rolling
blake
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Active
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CN201621220702.1U
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Chinese (zh)
Inventor
肖华春
王成业
汪鑫鑫
李玉梅
王楠楠
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Anhui Divinity Biological Products Co Ltd
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Anhui Divinity Biological Products Co Ltd
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Priority to CN201621220702.1U priority Critical patent/CN206219598U/en
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Abstract

The utility model discloses a kind of device for cell spinner bottle culture, it is characterized in that, described device includes blake bottle, rolling bottle, collecting vessel and modular converter, the modular converter is provided with inlet I, inlet II and liquid outlet, switch is equipped with the inlet I and inlet II, suction pipe I is provided between the inlet I and rolling bottle, the bottom of blake bottle is inserted in one end of the suction pipe I, and the other end is connected with inlet I;Suction pipe II is provided between the inlet II and collecting vessel, in one end insertion collecting vessel of the suction pipe II, the other end is connected with inlet II;Suction pipe III is connected with the liquid outlet, in the insertion rolling bottle of the suction pipe III;Peristaltic pump is housed on the suction pipe II;The blake bottle is provided with suction pipe IV, and in one end insertion blake bottle of the suction pipe IV, the other end is connected with air compressor.The utility model is simple to operate, is capable of achieving than smoother operation, and speed operations speed, reduces pollution rate.

Description

A kind of device for cell spinner bottle culture
Technical field
The utility model is related to a kind of device of cell culture, specifically a kind of device for cell spinner bottle culture, category In biological technical field.
Background technology
Rolling bottle (generally using 10 liters or 15 liters of serum bottles, the abbreviation rolling bottle) culture of cell, is mainly used in viral vaccine The increment of antigen harvests or changes the operation such as liquid, it is necessary to carry out passage, nutrient solution.If desired the liquid in rolling bottle(Bag Include cell culture fluid, cell dispersion liquid, PBS liquid etc.)When removing or collecting, typically directly the liquid of rolling bottle is directly poured out, Larger strength is needed, schoolgirl's operation has difficulties, and liquid easily spills and, and there is the hidden danger of pollution.If desired toward rolling bottle During interior addition liquid, then mouth to mouth in cell culture fluid addition rolling bottle.This mode is simple to operate, it also requires larger power Gas, schoolgirl's operation has difficulties, and boy student's operation is also inconvenient.And liquid is easily flowed out outside bottle, contaminated hidden danger is equally existed, And pass on, nutrient solution harvest or change liquid speed it is slow, it is necessary to staff is more, be unfavorable for large-scale production.In a word, existing skill Art is although simple to operate, but not easy to operate, and maximum problem is to be very easy to cause pollution.
The content of the invention
For the problem that above-mentioned prior art is present, the utility model provides a kind of device for cell spinner bottle culture, Can effectively solve the problem that liquid is easily contaminated and operates slack technical barrier.
To achieve these goals, a kind of device for cell spinner bottle culture that the utility model is used, its feature exists In described device includes blake bottle, rolling bottle, collecting vessel and modular converter, and the modular converter is provided with inlet I, inlet II and liquid outlet, switch is equipped with the inlet I and inlet II, it is provided with suction pipe between the inlet I and blake bottle I, the bottom of blake bottle is inserted in one end of the suction pipe I, and the other end is connected with inlet I;The inlet II and collecting vessel Between be provided with suction pipe II, in one end insertion collecting vessel of the suction pipe II, the other end is connected with inlet II;The liquid outlet On be connected with suction pipe III, in the bottom insertion rolling bottle of the suction pipe III;Being equipped with the suction pipe II is used for the liquid in rolling bottle The peristaltic pump of sucking-off;Suction pipe IV is provided with the top of the blake bottle, in one end insertion blake bottle of the suction pipe IV, the other end connects Be connected to the air compressor for the liquid in blake bottle being pressed into rolling bottle.
The suction pipe III is provided with bell jar, and the bell jar is sleeved on the bottleneck of rolling bottle.
One end of the suction pipe II is bent into 75 degree of angles.
The bottom of the suction pipe III is bent into 75 degree of angles.
The rolling bottle is horizontal positioned.
Described device also includes the matched bactericidal unit for using.
Compared with prior art, the utility model uses some simple instruments, including suction pipe, cover clock, peristaltic pump and pressure Contracting air engine etc., draws or collects liquid by suction pipe, and power is provided by peristaltic pump or air compressor, and duct type operation subtracts Few liquid can reduce the probability of pollution, it is not necessary to lift rolling bottle outside air, and effortlessly, ordinary person is operable, Simple and convenient, efficiency high is capable of achieving than smoother operation, and speed operations speed, reduces pollution rate.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model;
Wherein:1st, blake bottle;2nd, rolling bottle;3rd, collecting vessel;4th, modular converter;5th, bell jar;41st, inlet I;42nd, inlet Ⅱ;43rd, liquid outlet;6th, suction pipe I;7th, suction pipe II;8th, suction pipe III;9th, suction pipe IV;10th, air compressor;11st, peristaltic pump.
Specific embodiment
The utility model is described in further detail below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of device for cell spinner bottle culture, the device includes blake bottle 1, rolling bottle 2, collecting vessel 3 With modular converter 4.Inlet I 41, inlet II 41 and liquid outlet 43 are provided with modular converter 4, and in inlet I 41 and are entered Switch is equipped with liquid mouthful II 42, is switched for controlling the open-minded of suction pipe.Suction pipe I is provided between inlet I 41 and blake bottle 1 6, the bottom of blake bottle 1 is inserted in one end of suction pipe I 6, and the other end is connected with inlet I 41.In inlet II 42 and collecting vessel 3 Between be provided with suction pipe II 7, in one end insertion collecting vessel 3 of suction pipe II 7, the other end is connected with inlet II 42.In liquid outlet Suction pipe III 8 is connected with 43, in the bottom insertion rolling bottle 2 of suction pipe III 8.Being equipped with suction pipe II 7 is used for the liquid in rolling bottle 2 Suction out the peristaltic pump 11 that power is provided.Suction pipe IV 9 is provided with the top of blake bottle 1, in one end insertion blake bottle 1 of suction pipe IV 9, The other end is connected with the air compressor 10 for being pressed into the liquid in blake bottle in rolling bottle.
Further, suction pipe III 8 is provided with bell jar 5, and the bell jar 5 is sleeved on the bottleneck of rolling bottle 1.During design cover clock, Make the size of cover clock 5 that just the whole bottleneck of rolling bottle 2 be surrounded, when adding liquid bacterium can be avoided to fall into and dirt is caused in bottle Dye.
Further, cause the probability of pollution higher because bacterium falls into bottleneck by vertical approach, operating Journey need to as far as possible avoid bottleneck upwards, therefore, one end of suction pipe II 7 is bent into behind 75 degree of angles and the phase of inlet II 42 in the device Connection, the bottom of suction pipe III 8 is inserted in rolling bottle 2 after being bent into 75 degree of angles, and rolling bottle 2 is horizontal positioned.
Due to the process in operation, the pipeline of device and transport for holding liquid is needed in germ-free condition, because This, the device also includes the matched bactericidal unit for using.
Above example is only that, to explanation of the present utility model, it is not to limitation of the present utility model.For ability For the technical staff in domain, it can still modify to technical scheme described in previous embodiment, or carry out etc. With replacement.It is all it is of the present utility model spirit and principle in, any modification, equivalent substitution and improvements made etc. should be included in Within protection domain of the present utility model.

Claims (6)

1. a kind of device for cell spinner bottle culture, it is characterised in that described device includes blake bottle(1), rolling bottle(2), receive Collection bucket (3) and modular converter (4), the modular converter (4) are provided with inlet I (41), inlet II (42) and liquid outlet (43), the inlet I (41) and the place of inlet II (42) are equipped with switch, the inlet I (41) and blake bottle(1)Between It is provided with suction pipe I(6), the suction pipe I(6)One end insertion blake bottle(1)Bottom, the other end and inlet I(41)It is connected; The inlet II(42)And collecting vessel(3)Between be provided with suction pipe II(7), the suction pipe II(7)One end insertion collecting vessel(3) In, the other end and inlet II(42)It is connected;The liquid outlet(43)On be connected with suction pipe III(8), the suction pipe III(8)'s Insert rolling bottle in bottom(2)It is interior;The suction pipe II(7)The upper peristaltic pump equipped with for the liquid in rolling bottle to be suctioned out(11);It is described Blake bottle(1)Top be provided with suction pipe IV(9), the suction pipe IV(9)One end insertion blake bottle(1)Interior, the other end is connected with For the air compressor being pressed into the liquid in blake bottle in rolling bottle(10).
2. a kind of device for cell spinner bottle culture according to claim 1, it is characterised in that the suction pipe III(8) It is provided with bell jar(5), the bell jar(5)It is sleeved on rolling bottle(2)Bottleneck on.
3. a kind of device for cell spinner bottle culture according to claim 1, it is characterised in that the suction pipe II(7) One end be bent into 75 degree of angles.
4. a kind of device for cell spinner bottle culture according to claim 1, it is characterised in that the suction pipe III(8) Bottom be bent into 75 degree of angles.
5. a kind of device for cell spinner bottle culture according to claim 1, it is characterised in that the rolling bottle(2)For Horizontal positioned.
6. a kind of device for cell spinner bottle culture according to claim 1, it is characterised in that described device also includes The matched bactericidal unit for using.
CN201621220702.1U 2016-11-11 2016-11-11 A kind of device for cell spinner bottle culture Active CN206219598U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201621220702.1U CN206219598U (en) 2016-11-11 2016-11-11 A kind of device for cell spinner bottle culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201621220702.1U CN206219598U (en) 2016-11-11 2016-11-11 A kind of device for cell spinner bottle culture

Publications (1)

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CN206219598U true CN206219598U (en) 2017-06-06

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CN201621220702.1U Active CN206219598U (en) 2016-11-11 2016-11-11 A kind of device for cell spinner bottle culture

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CN (1) CN206219598U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111073803A (en) * 2020-01-19 2020-04-28 兆丰华生物科技(福州)有限公司 Spinner flask cell passage device and cell passage method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111073803A (en) * 2020-01-19 2020-04-28 兆丰华生物科技(福州)有限公司 Spinner flask cell passage device and cell passage method

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