CN205687905U - Direct PCR amplification kit - Google Patents
Direct PCR amplification kit Download PDFInfo
- Publication number
- CN205687905U CN205687905U CN201620605914.5U CN201620605914U CN205687905U CN 205687905 U CN205687905 U CN 205687905U CN 201620605914 U CN201620605914 U CN 201620605914U CN 205687905 U CN205687905 U CN 205687905U
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- CN
- China
- Prior art keywords
- capsule body
- centrifuge tube
- pcr amplification
- direct pcr
- amplification kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model discloses a kind of Direct PCR amplification kit, solve the technical problem that be easy for carrying, easy to use and it can be avoided that reagent contamination.This utility model test kit includes that box body, described test kit also include that centrifuge tube, respectively subpackage have capsule body and the grinding pestle of several reagent needed for Direct PCR amplification；The center of the lid of described centrifuge tube is provided with the pricker for puncturing capsule body upper and lower two ends end face, and the cross section of capsule body is circular, and the diameter of the cross section of capsule body is equal with the internal diameter of centrifuge tube, is provided with the chimb of one week annular in the periphery wall upper end of capsule body.Compared with prior art, this utility model Direct PCR amplification kit can avoid reagent to be contaminated；And the capsule body of respective amount can be carried as required, and the pricker that can puncture capsule body is set on centrifuge tube, easy to use, and low cost, it is possible to bring huge change to exempting from DNA extraction PCR, therefore there is good market application foreground.
Description
Technical field
This utility model relates to a kind of field of bioexperiment technology, particularly a kind of Direct PCR amplification kit.
Background technology
At present, traditional Direct PCR amplification kit, generally comprise and be respectively provided with solution A, solution B and PCR
The various Reagent Tubes of MagicMix 3.0, and in every kind of Reagent Tube, it is generally equipped with enough detecting the reagent of 100 times, and also it comprises
Grind pestle and experiment need use centrifuge tube be all scattering device, in use, by reagent by suction pipe from container
Centrifuge tube mixing is put in sucking-off, although this mode is simple, but is used for multiple times or carries and easily cause reagent contamination and lose
The problem of effect, and each reagent and consumptive material are all scattering devices, also need to coordinate liquid-transfering gun and the rifle head of different size during use,
It is the most convenient to operate.
Summary of the invention
The purpose of this utility model is to provide a kind of Direct PCR amplification kit, will solve the technical problem that and be easy for taking
Band, easy to use and it can be avoided that reagent contamination.
For solving the problems referred to above, this utility model realizes by the following technical solutions: a kind of Direct PCR amplification kit,
Described test kit includes that box body, described test kit also include that centrifuge tube, respectively subpackage have several examinations needed for Direct PCR amplification
The capsule body of agent and grinding pestle；The center of the lid of described centrifuge tube is provided with for puncturing capsule body upper and lower two ends end face
Pricker, the cross section of capsule body is circular, and the diameter of the cross section of capsule body is equal with the internal diameter of centrifuge tube, outside capsule body
Perisporium upper end is provided with the chimb of one week annular.
Several reagent needed for Direct PCR amplification kit described in the utility model include solution A, solution B and
PCR MagicMix 3.0 reagent.
Each capsule body of the present utility model is placed on and its shape adaptation and the capsule pipe that is easily extruded to centrifuge tube
In.
The lower end of capsule body described in the utility model is cambered surface.
The upper end of capsule body described in the utility model is provided with pellicular front.
The dividing plate that the inner chamber of box body is divided at least five compartment it is provided with in box body described in the utility model, described
Centrifuge tube, capsule body and grinding pestle are individually positioned in compartment.
It is provided with grinding pestle box in the compartment placing grinding pestle of the present utility model, grinds pestle and be placed in grinding pestle box,
Grind pestle box and be provided with lid.
Placement of the present utility model is provided with in the cavity equipped with the compartment of the capsule body of PCR MagicMix 3.0 reagent
Thermal insulation thermal insulation board.
It is provided with centrifuge tube bracket in the compartment of placement centrifuge tube described in the utility model, described centrifuge tube bracket sets
At least one is had to erect the putting hole of placement after being inserted by centrifuge tube.
This utility model Direct PCR amplification kit compared with prior art, uses each subregion will exempt from DNA extraction PCR
In required consumptive material and reagent Unified Set placed separately, then with a box body, the receiving of each subregion is come in, it is simple to carry；Use one
Quantitative exempt from DNA extraction PCR needed for several reagent be individually enclosed in capsule body, reagent can be avoided to be contaminated；And
The capsule body of respective amount can be carried as required, and the pricker that can puncture capsule body is set on centrifuge tube, easy to use, and
And low cost, it is possible to bring huge change to exempting from DNA extraction PCR, therefore have good market application foreground.
Accompanying drawing explanation
Fig. 1 is the structural representation of this utility model test kit.
Fig. 2 is the sectional view of this utility model centrifuge tube.
Fig. 3 is the sectional view of this utility model capsule body.
Fig. 4 is the structural representation of centrifuge tube during this utility model use and capsule body.
Detailed description of the invention
With embodiment, this utility model is described in further detail below in conjunction with the accompanying drawings.
As it is shown in figure 1, test kit of the present utility model includes box body 1, being provided with on box body 1 can uncovered by box body 1
The lid 12 closed, lid 12 realizes mutually fastening by the buckle structure of prior art with box body 1.
This test kit also includes centrifuge tube 2, is respectively provided with solution A, solution B and the PCR required for Direct PCR amplification
The capsule body 3 of MagicMix 3.0 and grinding pestle 4；Therein equipped with solution A, solution B and PCR MagicMix 3.0
The capacity of capsule body 3 can carry out fill according to the dosage that reality test is used；Of the present utility model exempt from DNA extraction PCR reagent
The model that solution A, solution B and PCR MagicMix 3.0 reagent source in box produces in precious Man is 130985-100
The reagent in DNA extraction PCR kit exempted from by type.
As in figure 2 it is shown, be provided with the thorn for puncturing capsule body about 3 two ends end face in the center of the lid of centrifuge tube 2
Pin 5；It is additionally provided with and the tube chamber of centrifuge tube 2 on a side end face relative with the lumen open of centrifuge tube 2 on the lid of centrifuge tube 2
The boss 13 of the suitable connection of opening.
As it is shown on figure 3, the cross section of capsule body 3 is circular, the diameter of its cross section is equal with the internal diameter of centrifuge tube 2, glue
The lower end of utricule 3 is cambered surface, and cambered surface can flow into centrifuge tube 2 with the liquid in guide capsule body 3 from the punctured hole of capsule body 3
In tube chamber；And the upper end of capsule body 3 is provided with the thickness pellicular front 15 less than capsule body 3 remainder thickness.Outside capsule body 3
Perisporium upper end is provided with the chimb 6 of one week annular, when capsule body 3 puts into centrifuge tube 2, and the lower edge of chimb 6 and centrifuge tube 2
Body upper end-face edge abut, so that capsule body 3 will not be fallen in the tube chamber of centrifuge tube 2.Each capsule body 3 can be put
Put with its shape adaptation and can easily extruding to the capsule pipe of centrifuge tube, to avoid depositing or be contaminated during the time,
Only need to clamp-on centrifuge tube by the bottom of extruding capsule pipe by one of capsule body 3 during use, utilize capsule pipe to be pressed by capsule body 3
Enter in centrifuge tube.
As it is shown in figure 1, be provided with the dividing plate 7 that the inner chamber of box body 1 is divided at least five compartment in box body 1, preferably 5
Individual, be respectively located at the centrifuge tube compartment 16 on the upside of box body 1 left side, be located at the grinding pestle compartment 17 on the downside of box body 1 left side,
Be located on the right of box body 1 upside for placing the first capsule body compartment 18 of the capsule body equipped with solution A, to be located at box body 1 right
Equipped with the second capsule body compartment 19 of the capsule body of solution B and being located on the downside of the right of box body 1 for placement in the middle part of limit
For placing the 3rd capsule body compartment 20 of capsule body equipped with PCR MagicMix 3.0, centrifuge tube 2 is placed on centrifugal
Pipe compartment 16, capsule body 3 is individually positioned in three capsule body compartments 18,19,20 according to the different reagent being contained in, and grinds
Pestle 4 is placed in grinding pestle compartment 17.
Because PCR MagicMix 3.0 needs low-temperature preservation, placing dividing of the capsule body 3 equipped with PCR MagicMix3.0
Being provided with thermal insulation thermal insulation board 9 in the cavity of separate space (the i.e. the 3rd capsule body compartment 20), this thermal insulation thermal insulation board 9 is by PE cotton outsourcing plastics
Material is made, and is also provided with the cover plate 14 being made by thermal insulation thermal insulation board, makes the 3rd on the 3rd capsule body compartment 20
Capsule body compartment 20 forms a cavity that can be used for low-temperature preservation.When needs go out to carry, the bag of-20 DEG C will be positioned over
Capsule pipe taking-up containing capsule body 3 is put into this utility model and is exempted from DNA extraction PCR kit, or will be positioned over-20 in advance
DEG C preserve this utility model Direct PCR amplification kit preserving capsule body 3 in directly take out, because of equipped with PCR
It is provided with thermal insulation thermal insulation board 9, even if going out, additionally it is possible to make it maintain in the cavity of the compartment of the capsule body 3 of MagicMix 3.0
At low temperature environment, it is to avoid lost efficacy.
It is provided with centrifuge tube bracket 10, centrifuge tube bracket in the compartment (i.e. centrifuge tube compartment 16) placing centrifuge tube 2
The two ends of 10 are fixed in the cavity wall of centrifuge tube compartment opposite sides, are provided with at least one for inciting somebody to action on centrifuge tube bracket 10
Centrifuge tube 2 erects the putting hole 11 of placement after inserting.
In grinding pestle compartment 17, it is also provided with the grinding pestle box 8 that can be taken off, grinds pestle box 8 and be provided with lid, can
So that it is cleaner, from pollution to grind pestle 3.
As shown in Figure 4, according to the order of addition of reagent, capsule body 3 is put into centrifuge tube 2, when the lid covering centrifuge tube 2
The period of the day from 11 p.m. to 1 a.m, pricker 5 punctures upper surface and the lower surface of capsule body 3 successively so that the thorn from lower surface of the reagent in capsule body 3
Holes flows in centrifuge tube 2, when being pressed on by the lid of centrifuge tube 2 further on the body of centrifuge tube 2, due to the table of boss 13
In the face of the extruding of the upper surface of capsule body 3, make the reagent in capsule body 3 be pushed completely in the tube chamber delivering to centrifuge tube 2, then take
Go out capsule body 3, repeat the above steps, make three kinds of reagent all be squeezed in the tube chamber of centrifuge tube 2.
Specifically used method is as follows:
Whether the capsule body 3 needing detailed inspection solution A and solution B when using this product first has crystallization.As
Fruit is rocked the most stand-by after there is a need to 37 DEG C of dissolvings.That does not breaks a seal can put room temperature preservation equipped with solution A and solution B capsule body 3.
2. take a small amount of sample in the centrifuge tube 2 of 1.5mL plastics according to material difference with reference to following table.
| Sample type | The amount of taking |
| Animal tissue | 1-5mg |
| Rat-tail | 2mm length |
| Blood sample | Less than 20uL |
| Plant leaf blade | 1-5mg |
| Plant seed (is removed the peel) | 1-5mg |
| Yeast culture | 0.2-0.5mL culture precipitates |
| Bacterial cultures | 0.2-0.5mL culture precipitates |
Noting: unlisted sample, user needs oneself to grope optimum amount.To rich in many quinones polyphenol vegetable material (as
Cotton Gossypii), tissue usage amount is preferably not more than 0.5mg.
3. adding 100uL solution A, the capsule body 3 that will be equipped with solution A is placed on centrifuge tube 2, closes the lid, and makes pricker 5 sting
Broken pellicular front 15 and the lower surface of capsule body 3, allow solution A flow in centrifuge tube 2, press centrifuge tube 2 lid, it is ensured that capsule body
Solution A in 3 is extruded completely and guarantees that sample is flowed or blow over and cover completely by solution A completely, and takes out used capsule body 3.
4. grind pestle 4 carefully to be smashed to pieces in centrifuge tube by sample.Blood typically smashs 30 seconds to pieces；Solid tissue needs to smash
The broken color to solution becomes cloudy (if plant leaf blade, the color of solution will become green).As needed more grinding pestles 4, can be another
Purchase.To thorny sample, it is also possible to increase a 10-60 minute step of 95 DEG C of insulations, enter back into next after temperature is cooled to room temperature
Step.The solid tissue fragment of residual does not interferes with follow-up test.
5. adding 100uL solution B, the capsule body 3 that will be equipped with solution B is placed on centrifuge tube 2, closes the lid, and makes pricker 5 sting
Broken pellicular front 15 and the lower surface of capsule body 3, allow solution B flow in centrifuge tube 2, press centrifuge tube 2 lid, it is ensured that capsule body
Solution B in 3 is extruded completely, takes out used capsule body 3, and centrifuge tube 2 shakes mixing in 10 seconds.
6. room temperature 12000rpm is centrifuged 2 minutes.
7. supernatant is transferred in a clean 1.5mL centrifuge tube.If need not ,-80 DEG C long-term guarantors can be put
Deposit.If for follow-up PCR, lower step please be directly entered.
Directly as follows as the PCR reaction system of template-setup 30uL system with lysate:
| Composition | Consumption |
| PCR MagicMix3.0 | 15uL |
| Cell pyrolysis liquid (comes in upper step) | 1-5uL |
| Provide PCR primer 1 (10uM) for oneself | 1uL |
| Provide PCR primer 2 (10uM) for oneself | 1uL |
| Moisturizing is arrived | 30uL |
Note: the capsule body 3 equipped with PCR MagicMix3.0 needs to put in centrifuge tube 2, punctures, make reagent flow into from
In heart pipe, lysate volume is preferably not more than the 1/10 of PCR volume.If needing to increase the addition of template, then PCR system
Should accordingly increase.If adding 5uL lysate, then can use the PCR system of 50uL.
Claims (9)
1. a Direct PCR amplification kit, described test kit includes box body (1), it is characterised in that: described test kit also includes
Centrifuge tube (2), respectively subpackage have capsule body (3) and grinding pestle (4) of several reagent needed for Direct PCR amplification；Described
The center of the lid of centrifuge tube (2) is provided with the pricker (5) for puncturing capsule body (3) two ends end face up and down, capsule body (3)
Cross section is circular, and the diameter of the cross section of capsule body (3) is equal with the internal diameter of centrifuge tube (2), at the periphery wall of capsule body (3)
Upper end is provided with the chimb (6) of one week annular.
Direct PCR amplification kit the most according to claim 1, it is characterised in that: described Direct PCR amplification kit institute
Several reagent needed include solution A, solution B and PCR MagicMix 3.0 reagent.
Direct PCR amplification kit the most according to claim 1, it is characterised in that: each capsule body (3) is placed on and it
Shape adaptation and be easily extruded to the capsule pipe of centrifuge tube (2).
Direct PCR amplification kit the most according to claim 2, it is characterised in that: the lower end of described capsule body (3) is arc
Face.
Direct PCR amplification kit the most according to claim 4, it is characterised in that: the upper end of described capsule body (3) is provided with
Pellicular front (15).
Direct PCR amplification kit the most according to claim 5, it is characterised in that: described box body is provided with box body in (1)
(1) inner chamber is divided into the dividing plate (7) of at least five compartment, described centrifuge tube (2), capsule body (3) and grinding pestle (4) point
It is not placed in compartment.
Direct PCR amplification kit the most according to claim 6, it is characterised in that: place in the compartment grinding pestle (4)
It is provided with grinding pestle box (8), grinds pestle (4) and be placed in grinding pestle box (8), grind pestle box (8) and be provided with lid.
Direct PCR amplification kit the most according to claim 7, it is characterised in that: place equipped with PCR MagicMix
It is provided with thermal insulation thermal insulation board (9) in the cavity of the compartment of the capsule body (3) of 3.0 reagent.
Direct PCR amplification kit the most according to claim 8, it is characterised in that: the separation of described placement centrifuge tube (2)
Intracavity is provided with centrifuge tube bracket (10), and described centrifuge tube bracket (10) is provided with at least one after being inserted by centrifuge tube (2)
Erect the putting hole (11) placed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620605914.5U CN205687905U (en) | 2016-06-20 | 2016-06-20 | Direct PCR amplification kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620605914.5U CN205687905U (en) | 2016-06-20 | 2016-06-20 | Direct PCR amplification kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205687905U true CN205687905U (en) | 2016-11-16 |
Family
ID=57415787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620605914.5U Expired - Fee Related CN205687905U (en) | 2016-06-20 | 2016-06-20 | Direct PCR amplification kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205687905U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108641899A (en) * | 2018-05-24 | 2018-10-12 | 金陵科技学院 | A kind of pre- point of lid of centrifuge tube |
| CN110068679A (en) * | 2018-01-23 | 2019-07-30 | 深圳市易瑞生物技术股份有限公司 | Immuno-chromatography detection device |
| CN113941384A (en) * | 2021-11-21 | 2022-01-18 | 洛阳职业技术学院 | Capsule type secondary centrifuge tube capable of being applied to rapid nondestructive analysis |
-
2016
- 2016-06-20 CN CN201620605914.5U patent/CN205687905U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110068679A (en) * | 2018-01-23 | 2019-07-30 | 深圳市易瑞生物技术股份有限公司 | Immuno-chromatography detection device |
| CN108641899A (en) * | 2018-05-24 | 2018-10-12 | 金陵科技学院 | A kind of pre- point of lid of centrifuge tube |
| CN113941384A (en) * | 2021-11-21 | 2022-01-18 | 洛阳职业技术学院 | Capsule type secondary centrifuge tube capable of being applied to rapid nondestructive analysis |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Fu Wei Inventor after: Li Xinshi Inventor after: Wei Shuang Inventor after: Wang Tianjie Inventor after: Wang Chenguang Inventor after: Zhu Pengyu Inventor after: Lin Chungui Inventor after: Cai Xueyan Inventor after: Zhu Shuifang Inventor before: Fu Wei Inventor before: Wei Shuang Inventor before: Wang Tianjie Inventor before: Wang Chenguang Inventor before: Zhu Pengyu Inventor before: Lin Chungui Inventor before: Cai Xueyan Inventor before: Zhu Shuifang |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161116 Termination date: 20180620 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |