CN205263096U - Trace immunity chromatography detects card - Google Patents
Trace immunity chromatography detects card Download PDFInfo
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- CN205263096U CN205263096U CN201521050427.9U CN201521050427U CN205263096U CN 205263096 U CN205263096 U CN 205263096U CN 201521050427 U CN201521050427 U CN 201521050427U CN 205263096 U CN205263096 U CN 205263096U
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- test paper
- card
- panel
- skeptophylaxis
- carminative
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Abstract
The utility model discloses a trace immunity chromatography detects card. Trace immunity chromatography detects card includes panel and bottom plate, and the panel is in the same place with the bottom plate lock, is equipped with immunity chromatography trace test paper between panel and the bottom plate, be equipped with carminative hole, sample well and observation window on the panel in proper order, the inboard of bottom plate is equipped with the fixed unit of test paper, be fixed in the bottom plate through the fixed unit of test paper with immunity chromatography trace test paper on, the inboard of panel is equipped with the test paper and compresses tightly the unit, and the test paper compresses tightly the straight line setting that carminative hole, sample well and observation window place are followed to the unit, is used for compressing tightly immunity chromatography trace test paper through compressing tightly the unit. The utility model discloses trace immunity chromatography detects card is provided with carminative hole and sample well, can part sample solution and carminative, can greatly reduce required sample size, realizes that the trace of immunity chromatography test paper detects, satisfies some samples and precious very much or acquire special circumstances's such as difficult very much demand.
Description
Technical field
The utility model relates to a kind of skeptophylaxis chromatography test card.
Background technology
Immuno-chromatographic assay technology rises in the nineties in last century, is able in recent years fast development. This technology both canRealize qualitative detection taking collaurum, electroselenium and color latex microballoon as label, also can with fluorescence latex beads,Superparamagnetism microballoon, quantum dot are that label is realized quantitatively detection. Easy to carry, operation that immunochromatographydetection detection card hasSimply, detect the advantages such as quick, with low cost, be widely used in medical treatment, food, chemistry, the field such as biological.
On existing immunochromatographydetection detection card panel, only have two holes, one is well, and another is observation window, willSample drips in sample pad by well, by blotting paper active force, sample slowly moves in test strips,Finally observe the colored variation of bar on nitrocellulose filter by observation window and realize specific immune detection, thisAdd the needed sample size of quadrat method larger, sample solution will flow to the other end from one end of test strips, soaks into completelyWhole test strips, causes sample solution to waste greatly, and in actual applications, runs into sometimes sample precious especiallyOr obtain the situation of difficulty especially, existing immunochromatographydetection detection card cannot meet the demand of trace detection.
Utility model content
The purpose of this utility model is to provide a kind of skeptophylaxis chromatography test card, has overcome existing immunochromatographydetection detection cardCannot realize the defect of trace detection.
Skeptophylaxis chromatography test card provided by the utility model, comprises a panel and a base plate, described panel and instituteState base plate and snap together, between described panel and described base plate, be provided with immunochromatography trace detection test paper;
On described panel, be provided with successively carminative hole, sample well and observation window, and described carminative hole, described sample wellWith described observation window respectively with carminative pad, sample pad and the nitrocellulose filter of described immunochromatography trace detection test paperCorresponding;
The inner side of described base plate is provided with test paper fixed cell, by described test paper fixed cell by described immunochromatography traceTest paper is fixed on described base plate;
The inner side of described panel is provided with test paper compressing unit, and described test paper compressing unit is along described carminative hole, described sampleThe straight line setting at product hole and described observation window place, is used for compressing the inspection of described immunochromatography trace by described compressing unitTest paper.
In described skeptophylaxis chromatography test card, described panel and described base plate are realized and being joined by locating hole and locating dowelClose, as described in panel and as described in the inner side of base plate all arrange as described in locating hole and as described in locating dowel.
In described skeptophylaxis chromatography test card, described test paper fixed cell comprises draw-in groove A, draw-in groove B, bracing frameA, bracing frame B and brace summer, draw-in groove A and draw-in groove B lay respectively at the two ends of described base plate, described brace summer and instituteThe position of stating observation window is corresponding, and support frame as described above A is corresponding with the position in described carminative hole, described immunochromatographyTrace detection test paper is located at the top of support frame as described above A, support frame as described above B and described brace summer, and two ends are fixed onIn described draw-in groove A and draw-in groove B.
In described skeptophylaxis chromatography test card, along the opening direction of described draw-in groove A, support frame as described above A is inclinationShape, and then can make the carminative adding be stored in the inside of described draw-in groove A.
In described skeptophylaxis chromatography test card, described test paper compressing unit comprise card column A, card A, card B,Card C and 2 card column B;
Described card column A is located at the one end in described carminative hole, and away from described sample well setting; Described card A and cardSheet B is located at respectively the two ends of described sample well, and described card C and described 2 card column B are located at respectively described observation windowTwo ends.
In described skeptophylaxis chromatography test card, described panel is provided with decoration grid, plays decoration and anti-skidding.
In described skeptophylaxis chromatography test card, on described panel, be provided with the mark of detection line in a side of described observation windowKnow the mark C of T and nature controlling line, correspond respectively to detection line and the nature controlling line of described immunochromatography trace detection test paper.
In described skeptophylaxis chromatography test card, described carminative hole is round rectangle funnel-form, and described sample well isCircular funnel-form, the described observation window shape that is rectangle.
The skeptophylaxis chromatography test card that the utility model provides, is provided with carminative hole and sample well, can sample is moltenLiquid and carminative separately, can greatly reduce required sample size, realize the trace detection of immune chromatography test paper, meetSome samples are precious especially or obtain the demand of the special circumstances such as difficulty especially.
Brief description of the drawings
Fig. 1 is the front view of the utility model skeptophylaxis chromatography test card.
Fig. 2 is the panel top view of the utility model skeptophylaxis chromatography test card.
Fig. 3 is the panel cut-away view of the utility model skeptophylaxis chromatography test card.
Fig. 4 is the baseplate internal structure figure of the utility model skeptophylaxis chromatography test card.
In figure, each mark is as follows:
100 panels; 101 carminative holes; 102 wells; 103 observation windows; 104 detection line marks; 105 matterControl line mark; 106 decorate grid; 107 card column A; 108 card A; 109 card B; 110 card C; 111Card column B; 112 locating holes; 113 locating dowels; 200 base plates; 201 draw-in groove A; 202 bracing frame A; 203Support beam; 204 bracing frame B; 205 draw-in groove B; 206 locating holes; 207 locating dowels.
Detailed description of the invention
Below in conjunction with accompanying drawing, the utility model is described further, but the utility model is not limited to following examples.
Fig. 1 and Fig. 2 are the external structure schematic diagrames of the utility model embodiment skeptophylaxis chromatography test card.
As shown in Figure 1, 2, the utility model skeptophylaxis chromatography test card comprises panel 100 and closely fastens with itBase plate 200, test strips is fixed on the centre of panel 100 and base plate 200, panel 100 and base plate 200 are by establishingPut locating dowel 113 on panel 100, locating hole 112 and the locating hole 206 on corresponding base plate 200, fixed with itPosition post 207 snaps together. On described panel 100, be provided with carminative hole 101, sample well 102, observation window103 and decorate grid 106; Observation window 103 1 sides are provided with the mark " T " of detection line and the mark " C " of nature controlling line. DriveMoving agent hole 101 is the design of round rectangle funnel-form, the rounded funnel-form design of sample well 102, the shape of observation window 103Shape is rectangle, decorates grid 106 and has 5, from low to high, be short to long being arranged in order. Panel 100 correspondencesThe part of test strips is convex, and projection is around according to the shape of carminative hole 101, sample well 102 and observation window 103Shape is airflow design.
Fig. 3 is the panel cut-away view of the utility model skeptophylaxis chromatography test card. As shown in Figure 3, panel 100Inner side is provided with compressing unit, comprises a card column A107, is placed in the left side in carminative hole 101, for compressing test paperOne end, three cards: card A108, card B109 and card C110, be placed in respectively sample well 102 the left side,The right and the left side of observation window 103, two card column B111 side by side, are placed in the right of observation window 103, are used for respectivelyCompress test paper junction.
Fig. 4 is the baseplate internal structure figure of the utility model skeptophylaxis chromatography test card. As shown in Figure 4, base plate 200Inner side is provided with test paper fixed cell, comprises two draw-in grooves: draw-in groove A201 and draw-in groove B205, and for blocking test stripsTwo ends, two bracing frames (bracing frame A202 and bracing frame B204) and a brace summer 203, for holding up wholeTest strips. The bracing frame A202 position corresponding with carminative hole 101 on panel 100 is " slope " shape, canMake the carminative adding be stored in the inside of draw-in groove 201, slowly flow to the other end of test strips, coutroi velocity.
In the present embodiment, the long 71mm of skeptophylaxis chromatography test card, wide 22mm, with not containing background fluorescence signalMake with the ABS engineering plastics of background magnetic.
Use the present embodiment skeptophylaxis chromatography test card to detect:
Embodiment 1,
Hg immunochromatography qualitative detection test paper is (by Vegetable Research Centre, Beijing Academy of Agriculture and Forest Sciences's development and production, collaurumMethod) be cut into 4mm width, be placed in the middle of panel 100 of the present utility model and base plate 200, fixing, compress, addDrier, Hg skeptophylaxis chromatography qualitative detection card. While carrying out pattern detection, get 1 μ l sample and 1 μ lEDTASolution (concentration 2mM/L) mixes, and in dripping, in inner test strips, then drives by adding by sample well 102Moving agent hole 101 adds 150 μ l deionized waters as carminative, under the effect of test strips other end blotting paper, go fromSub-water-driven sample slowly moves in test strips inside, waits for 5 minutes, can be by naked eyes in the interior sight of observation window 103Examine testing result. Negative: C line and T line all develop the color, and shade is identical, the positive: the colour developing of C line, T line is notColour developing, or the color of T line is weaker than C line, and T line shoals along with the increase of Hg ion concentration in sample.
Embodiment 2,
Hg immunochromatography quantitative testing test paper (have Vegetable Research Centre, Beijing Academy of Agriculture and Forest Sciences's development and production, divide by the timeDistinguish fluorescent microsphere method) be cut into 4mm width, be placed in the middle of panel 100 of the present utility model and base plate 200, fixing,Compress, add drier, Hg skeptophylaxis chromatography quantitative test card. While carrying out pattern detection, get 1 μ l sampleThis and 1 μ lEDTA solution (concentration 2mM/L) mix, drips in inner test strips by sample well 102,Then add 150 μ l deionized waters as carminative by adding carminative hole 101, at test strips other end blotting paperUnder effect, deionized water drives sample slowly to move in test strips inside, waits for 5 minutes, inserts fluorescent quantitation and detectsIn the test paper hole clipping of instrument, instrument reads the fluorescence signal intensity of C, T line automatically, by the built-in calibration curve of instrumentEquation, calculates the concentration value of contained Hg ion in sample.
Claims (8)
1. a skeptophylaxis chromatography test card, is characterized in that: it comprises a panel and a base plate, described panelSnap together with described base plate, between described panel and described base plate, be provided with immunochromatography trace detection test paper;
On described panel, be provided with successively carminative hole, sample well and observation window, and described carminative hole, described sample wellWith described observation window respectively with carminative pad, sample pad and the nitrocellulose filter of described immunochromatography trace detection test paperCorresponding;
The inner side of described base plate is provided with test paper fixed cell, by described test paper fixed cell by described immunochromatography traceTest paper is fixed on described base plate;
The inner side of described panel is provided with test paper compressing unit, and described test paper compressing unit is along described carminative hole, described sampleThe straight line setting at product hole and described observation window place, is used for compressing the inspection of described immunochromatography trace by described compressing unitTest paper.
2. skeptophylaxis chromatography test card according to claim 1, is characterized in that: described panel with described inBase plate is realized and being coordinated by locating hole and locating dowel.
3. skeptophylaxis chromatography test card according to claim 1 and 2, is characterized in that: described test paper is solidOrder unit comprises draw-in groove A, draw-in groove B, bracing frame A, bracing frame B and brace summer, and draw-in groove A and draw-in groove B be position respectivelyIn the two ends of described base plate, described brace summer is corresponding with the position of described observation window, support frame as described above A with described in driveThe position in moving agent hole is corresponding, and described immunochromatography trace detection test paper is located at support frame as described above A, support frame as described above BWith the top of described brace summer, and two ends are fixed in described draw-in groove A and draw-in groove B.
4. skeptophylaxis chromatography test card according to claim 3, is characterized in that: along described draw-in groove A'sOpening direction, support frame as described above A is skewed.
5. skeptophylaxis chromatography test card according to claim 1 and 2, is characterized in that: described test paper is pressedClamping unit comprises card column A, card A, card B, card C and 2 card column B;
Described card column A is located at the one end in described carminative hole, and away from described sample well setting; Described card A and cardSheet B is located at respectively the two ends of described sample well, and described card C and described 2 card column B are located at respectively described observation windowTwo ends.
6. skeptophylaxis chromatography test card according to claim 1 and 2, is characterized in that: on described panelBe provided with decoration grid.
7. skeptophylaxis chromatography test card according to claim 1 and 2, is characterized in that: on described panelBe provided with the mark T of detection line and the mark C of nature controlling line in a side of described observation window, correspond respectively to described immunity layerAnalyse detection line and the nature controlling line of trace detection test paper.
8. skeptophylaxis chromatography test card according to claim 1 and 2, is characterized in that: described carminativeHole is round rectangle funnel-form, the rounded funnel-form of described sample well, the described observation window shape that is rectangle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521050427.9U CN205263096U (en) | 2015-12-16 | 2015-12-16 | Trace immunity chromatography detects card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521050427.9U CN205263096U (en) | 2015-12-16 | 2015-12-16 | Trace immunity chromatography detects card |
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| Publication Number | Publication Date |
|---|---|
| CN205263096U true CN205263096U (en) | 2016-05-25 |
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| CN201521050427.9U Expired - Fee Related CN205263096U (en) | 2015-12-16 | 2015-12-16 | Trace immunity chromatography detects card |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106405079A (en) * | 2016-11-18 | 2017-02-15 | 百奥森（江苏）食品安全科技有限公司 | Nine-link detection card for beta-incitant |
| CN110133264A (en) * | 2019-06-11 | 2019-08-16 | 上海雄图生物科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed |
| CN110361529A (en) * | 2019-07-19 | 2019-10-22 | 广东盛泽康华生物医药有限公司 | A kind of reagent card, detection method and application |
-
2015
- 2015-12-16 CN CN201521050427.9U patent/CN205263096U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106405079A (en) * | 2016-11-18 | 2017-02-15 | 百奥森（江苏）食品安全科技有限公司 | Nine-link detection card for beta-incitant |
| CN110133264A (en) * | 2019-06-11 | 2019-08-16 | 上海雄图生物科技有限公司 | A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed |
| CN110361529A (en) * | 2019-07-19 | 2019-10-22 | 广东盛泽康华生物医药有限公司 | A kind of reagent card, detection method and application |
| CN110361529B (en) * | 2019-07-19 | 2024-04-19 | 广东盛泽康华生物医药有限公司 | Reagent card, detection method and application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160525 Termination date: 20181216 |