CN106405079A - Nine-link detection card for beta-incitant - Google Patents

Nine-link detection card for beta-incitant Download PDF

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Publication number
CN106405079A
CN106405079A CN201611015106.4A CN201611015106A CN106405079A CN 106405079 A CN106405079 A CN 106405079A CN 201611015106 A CN201611015106 A CN 201611015106A CN 106405079 A CN106405079 A CN 106405079A
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CN
China
Prior art keywords
beta
card
sample
stimulants
pad
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201611015106.4A
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Chinese (zh)
Inventor
胡彬
张进
吴念绮
周朱晨
张根义
周合
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100 Olson Jiangsu Food Safety Technology Co Ltd
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100 Olson Jiangsu Food Safety Technology Co Ltd
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Application filed by 100 Olson Jiangsu Food Safety Technology Co Ltd filed Critical 100 Olson Jiangsu Food Safety Technology Co Ltd
Priority to CN201611015106.4A priority Critical patent/CN106405079A/en
Publication of CN106405079A publication Critical patent/CN106405079A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a nine-link detection card for beta-incitant. The nine-link detection card comprises a shell and a detection card arranged in the shell, wherein the shell comprises an upper shell and a lower shell; a detection card accommodating cavity is formed between the upper shell and the lower shell; four corners of the lower shell are provided with bosses; the bosses are provided with clamping holes; the upper shell is provided with clamping posts corresponding to the bosses, and the clamping posts are matched with the clamping holes in the bosses; the detection card comprises a bottom plate; the middle of the top of the bottom plate is provided with nine reaction films; one side of each reaction film is provided with a sample pad and a structure release pad, and the other side of each reaction film is provided with a water absorption pad; the tops of the sample pads, the structure release pads and the water absorption pads are provided with fixing films; the parts, corresponding to the middles of the sample pads, of the fixing films are provided with sample feeding holes; nine sample pads and nine reaction films are arranged; the nine sample pads are respectively used for detecting the clenbuterol hydrochloride, ractopamine, salbutamol, cimbuterol, mabuterol, bombuterol, tulobuterol, terbutaline and clorprenaline in the beta-incitant; positions corresponding to the reaction films are observation areas.

Description

Card is surveyed in a kind of beta-stimulants nine joint inspection
Technical field
The present invention relates to biological technical field, specifically a kind of beta-stimulants nine joint inspection survey card.
Background technology
Beta-stimulants(β-agonist)Full name receptor,β agonist(β-adrenoceptor agonist), It is beta-2-agonists again, be the phenethylamine class medicine of class chemical constitution epinephrine similar with physiological function and norepinephrine Thing.Common beta-stimulants are clenbuterol, Ractopamine, albuterol, cimaterol, Mabuterol, fenoterol, spy Bu Talin etc..Beta-stimulants can be optionally combined with the receptor,β on cell membrane, plays regulation sympathetic nerve emerging Put forth energy, the function of lax tracheal smooth muscle, be therefore clinically usually used in the treatment of humans and animals asthma class disease.80 years 20th century In generation, there are some researches show, beta-stimulants affect nutrient substance flow direction in animal body and redistribute, and make internal steatolysiss Metabolism enhancing, protein synthesis increase, and significantly improve lean meat percentage.Beta-stimulants residual can be gathered in animal edible tissues, Because this kind of compound has Orally active, if illegal Use out of range will produce to internal organs such as the liver of people and animal, kidneys Raw toxic and side effects.Therefore this kind of material is classified as the chemical substance forbidden for food animal by countries in the world.
Content of the invention
The present invention is intended to provide card is surveyed in a kind of beta-stimulants nine joint inspection.
For realizing described purpose, the present invention provides following technical scheme:Card is surveyed in a kind of beta-stimulants nine joint inspection, including housing With the detection card in housing, described housing includes upper casing and lower casing, forms detection card accommodating chamber, institute between upper casing and lower casing State lower casing corner and be additionally provided with boss, boss offers hole clipping;Described upper casing is provided with the card column corresponding with boss, card column with Hole clipping on boss matches;
Described detection card includes base plate, and base plate top centre position is provided with reaction film, and reaction film side is provided with sample pad and structure Thing release pad, reaction film opposite side is provided with adsorptive pads, and described sample pad, works release pad and adsorptive pads top are equipped with fixation Film, fixing film is provided with well with sample pad medium position corresponding position;
Described sample pad and reaction film have nine, and nine sample pad are respectively and detect clenobuterol hydrochloride, Rec in beta-stimulants The sample of dopamine, albuterol, western Boot sieve, Mabuterol, bromine Boot sieve, Tulobuterol, terbutaline and clorprenaline Pad;The corresponding position of described reaction film is observation area.
Preferably, described lower casing corner is provided with right angle limiting plate.
Preferably, described upper casing is provided with sample-adding port lid.
Preferably, it is additionally provided with timing and alarming device in described housing.
Compared with prior art, the invention has the beneficial effects as follows:
1st, present configuration is simple, novel, can detect simultaneously clenobuterol hydrochloride in beta-stimulants, Ractopamine, The residual of albuterol, western Boot sieve, Mabuterol, bromine Boot sieve, Tulobuterol, terbutaline and clorprenaline, detection speed Degree is fast, and Detection results are good.
2nd, lower casing corner is provided with and is stuck in housing not it is ensured that detecting for limiting the right angle limiting plate that detection card moves left and right Can be moved, it is to avoid impact routine testing.
3rd, upper casing is provided with sample-adding port lid, can close well when not in use, prevents environmental liquids from entering well, Impact testing result.
4th, it is additionally provided with timing and alarming device in housing, opening timing and alarming device to after well Deca sample liquid, reaching 5min When automatic alarm, remind staff observe testing result, prevent time-out.
Brief description
Fig. 1 is the internal structure schematic diagram that card is surveyed in a kind of beta-stimulants nine joint inspection provided by the present invention;
Fig. 2 is the front view that card is surveyed in a kind of beta-stimulants nine joint inspection provided by the present invention.
Shown in figure:1st, lower casing, 2, upper casing, 3, boss, 4, card column, 5, right angle limiting plate, 6, base plate, 7, observation area, 8, Well, 9, works release pad, 10, sample pad, 11, fixing film, 12, reaction film, 13, adsorptive pads, 14, sample-adding port lid, 15, Timing and alarming device.
Specific embodiment
The enforcement it is clear that described will be clearly and completely described to the technical scheme in the embodiment of the present invention below Example is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common The every other embodiment that technical staff is obtained under the premise of not making creative work, broadly falls into the model of present invention protection Enclose.
Refer to Fig. 1-2, in the embodiment of the present invention, card is surveyed in a kind of beta-stimulants nine joint inspection, including housing with located at housing Interior detection card, described housing includes upper casing 2 and lower casing 1, forms detection card accommodating chamber, described lower casing between upper casing 2 and lower casing 1 1 four jiaos are provided with for limiting the detection right angle limiting plate 5 that moves left and right of card it is ensured that detection is stuck in housing and will not be moved, Avoid affecting routine testing.1 four jiaos of described lower casing is additionally provided with boss 3, and boss 3 offers hole clipping;Described upper casing 2 be provided with The corresponding card column of boss 34, card column 4 is matched with the hole clipping on boss 3, so that upper casing 2 and lower casing 1 is connected together.
Described detection card includes base plate 6, and base plate 6 crown center position is provided with reaction film 12, and reaction film 12 side is provided with sample Product pad 10 and works release pad 9, reaction film 12 opposite side is provided with adsorptive pads 13, described sample pad 10, works release pad 9 and Adsorptive pads 13 top is equipped with fixing film 11, and fixing film 11 is provided with well 8 with sample pad 10 medium position corresponding position.
Described sample pad 10 can fully absorb detection liquid and gold labeling antibody completely attaches to and fully reacts, then chromatograph to Reacted on reaction film 12, can effectively be reduced error.It is also prevented from detecting that in sample, some interfering components make gold mark Albumen in antibody loses activity, the combination of impact gold labeling antibody and coating antigen.
Described sample pad 10 and reaction film 12 have nine, and nine sample pad 10 are respectively and detect hydrochloric acid gram in beta-stimulants Lun Teluo, Ractopamine, albuterol, western Boot sieve, Mabuterol, bromine Boot sieve, Tulobuterol, terbutaline and chlorine third The sample pad 10 of that woods.The corresponding position of described reaction film 12 is observation area 7.
Article nine, reaction film 12 is equipped with T line and C line, T line is detection line, C line is control line, by observing T line and C line Colour developing situation, so that it is determined that testing result is positive or negative;
The operation principle of the present invention is:When the beta-stimulants in sample are combined with gold labeling antibody, and then close in gold labeling antibody The antigen-combining site of beta-stimulants, is stoped gold labeling antibody from being combined with beta-stimulants conjugate on cellulose membrane T line position, makes T line Do not develop the color;Conversely, as do not contained beta-stimulants in sample liquid, then can not stop β on gold labeling antibody and cellulose membrane T line position-emerging Put forth energy agent conjugate combine, thus assuming aubergine on T line position.
The using method of the present invention is as follows:
With 3 bubble-free blood of the vertical Deca of plastic suction pipe(About 60-80ul)In well 8, treat that liquid is climbed to observation area 7; Display result is observed, after 10min, result is meaningless in 5min.
Described upper casing 2 is provided with sample-adding port lid 14, can close well 8 when not in use, prevents environmental liquids from entering Well 8, affects testing result.
It is additionally provided with timing and alarming device 15 in described housing, opening timing and alarming device 15 to after well 8 Deca sample liquid, arriving Reach automatic alarm during 5min, remind staff to observe testing result, prevent time-out.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto, Any those familiar with the art the invention discloses technical scope in, technology according to the present invention scheme and its Inventive concept equivalent or change in addition, all should be included within the scope of the present invention.

Claims (4)

1. card is surveyed in a kind of beta-stimulants nine joint inspection, and the detection card including housing and in housing is it is characterised in that described housing Including upper casing(2)And lower casing(1), upper casing(2)And lower casing(1)Between formed detection card accommodating chamber, described lower casing(1)Corner also sets There is boss(3), boss(3)On offer hole clipping;Described upper casing(2)It is provided with and boss(3)Corresponding card column(4), card column (4)With boss(3)On hole clipping match;
Described detection card includes base plate(6), base plate(6)Crown center position is provided with reaction film(12), reaction film(12)Side sets There is sample pad(10)With works release pad(9), reaction film(12)Opposite side is provided with adsorptive pads(13), described sample pad(10)、 Works release pad(9)And adsorptive pads(13)Top is equipped with fixing film(11), fixing film(11)Go up and sample pad(10)Middle part Position corresponding position is provided with well(8);
Described sample pad(10)And reaction film(12)There are nine, nine sample pad(10)It is respectively hydrochloric acid in detection beta-stimulants Clenbuterol, Ractopamine, albuterol, western Boot sieve, Mabuterol, bromine Boot sieve, Tulobuterol, terbutaline and chlorine The sample pad of third that woods(10);Described reaction film(12)Corresponding position is observation area(7).
2. beta-stimulants nine joint inspection according to claim 1 surveys card it is characterised in that described lower casing(1)Corner is provided with directly Angle limiting plate(5).
3. beta-stimulants nine joint inspection according to claim 1 surveys card it is characterised in that described upper casing(2)It is provided with sample-adding Port lid(14).
4. beta-stimulants nine joint inspection according to claim 1 surveys card it is characterised in that being additionally provided with timing report in described housing Alert device(15).
CN201611015106.4A 2016-11-18 2016-11-18 Nine-link detection card for beta-incitant Pending CN106405079A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611015106.4A CN106405079A (en) 2016-11-18 2016-11-18 Nine-link detection card for beta-incitant

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Application Number Priority Date Filing Date Title
CN201611015106.4A CN106405079A (en) 2016-11-18 2016-11-18 Nine-link detection card for beta-incitant

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CN106405079A true CN106405079A (en) 2017-02-15

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101477124A (en) * 2008-12-17 2009-07-08 北京望尔康泰生物技术有限公司 Method for detecting melamine and its special test paper
CN202256342U (en) * 2011-09-29 2012-05-30 广州瑞森生物科技有限公司 Rapid detection card for detecting beta agonist residues in animal blood
CN204575661U (en) * 2015-05-13 2015-08-19 杭州傲敏生物科技有限公司 Beta-stimulants nine test card
CN204594994U (en) * 2015-04-24 2015-08-26 苏州快捷康生物技术有限公司 A kind of beta-agonist immune chromatography test card
CN205263096U (en) * 2015-12-16 2016-05-25 北京市农林科学院 Trace immunity chromatography detects card
CN205581117U (en) * 2016-04-05 2016-09-14 杭州泰熙生物技术有限公司 Beta - drug -testing card
CN106053797A (en) * 2016-07-18 2016-10-26 河南华程氏生物科技股份有限公司 Chromatographic analysis system
CN206348341U (en) * 2016-11-18 2017-07-21 百奥森(江苏)食品安全科技有限公司 Card is surveyed in a kind of joint inspection of beta-agonist nine

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101477124A (en) * 2008-12-17 2009-07-08 北京望尔康泰生物技术有限公司 Method for detecting melamine and its special test paper
CN202256342U (en) * 2011-09-29 2012-05-30 广州瑞森生物科技有限公司 Rapid detection card for detecting beta agonist residues in animal blood
CN204594994U (en) * 2015-04-24 2015-08-26 苏州快捷康生物技术有限公司 A kind of beta-agonist immune chromatography test card
CN204575661U (en) * 2015-05-13 2015-08-19 杭州傲敏生物科技有限公司 Beta-stimulants nine test card
CN205263096U (en) * 2015-12-16 2016-05-25 北京市农林科学院 Trace immunity chromatography detects card
CN205581117U (en) * 2016-04-05 2016-09-14 杭州泰熙生物技术有限公司 Beta - drug -testing card
CN106053797A (en) * 2016-07-18 2016-10-26 河南华程氏生物科技股份有限公司 Chromatographic analysis system
CN206348341U (en) * 2016-11-18 2017-07-21 百奥森(江苏)食品安全科技有限公司 Card is surveyed in a kind of joint inspection of beta-agonist nine

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Application publication date: 20170215