CN110133264A - A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed - Google Patents
A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed Download PDFInfo
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- CN110133264A CN110133264A CN201910499683.2A CN201910499683A CN110133264A CN 110133264 A CN110133264 A CN 110133264A CN 201910499683 A CN201910499683 A CN 201910499683A CN 110133264 A CN110133264 A CN 110133264A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
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Abstract
A kind of time-resolved fluoroimmunoassay detecting Mycotoxins in Feed chromatographs kit, including box body, reagent strip, box cover, the first sealing-plug, the second sealing-plug, catheter and for the tightening component of fixating reagent item；Place reagent strip in the bottom of box body；Reagent strip compresses tightening component towards the end face of stripper plate respectively；Tightening component is slidably connected box body respectively；Box cover is equipped with the liquid feeding groove for sample to be tested to be added dropwise to reagent strip and the observation groove for observing reagent strip；Box cover is fastened and connected box body；The bottom of liquid feeding groove is respectively equipped with the second mounting hole；Catheter is threaded into respectively in the second mounting hole；Second sealing-plug is detachably arranged in liquid feeding groove；The bottom of observation groove is respectively equipped with third mounting hole；First sealing-plug is detachably arranged in observation groove.Kit provided by the invention is convenient to use, to reagent strip can be rapidly secured in box body；Box body and box cover easy disassembly, and it is avoided that foreign matter is fallen on reagent strip.
Description
Technical field
The present invention relates to detection kit technical field more particularly to a kind of time resolutions for detecting Mycotoxins in Feed
Fluorescence immune chromatography kit.
Background technique
Aflatoxin B1 is the derivative of dihydrofuran cumarin, has strong carcinogenicity and hypertoxicity, is so far
One kind most stable in the various mycotoxins of the present discovery, carcinogenicity is strongest.State General Administration for Quality Supervision provides aflatoxin B1
It is one of the essential items for inspection of most of food." the mycotoxin in national food safety standard food currently, GB 2761-2017
Limitation " in in foodstuff grain and its product aflatoxin B1 highest limitation be 20 μ g/kg.GB13078-2001 " feed
Sanitary standard " in be 10-50 μ g/kg to the highest limit standard of aflatoxin B1 in different types of feed.Cattle and sheep pig is raised
Material, corn, dregs of beans, DDGS(vinasse albumen feed) etc. the threat polluted by aflatoxin B1 of food, especially southern
High temperature, high Humid Area are contaminated the most serious.
Currently, aflatoxin B1, which usually passes through fluorescence immunoassay, quantifies the detection of POCT analyzer；In use, will test test paper
Item is placed in detection box；But when in use, checkbox is inconvenient to dismount, more inconvenient to be rapidly secured to test strip
In box body；In addition, falling in the foreign matter in test strip and falling the foreign matter in box body peep hole or well
It will affect the accuracy of testing result.
Summary of the invention
(1) goal of the invention
To solve technical problem present in background technique, the present invention proposes a kind of time resolution for detecting Mycotoxins in Feed
Fluorescence immune chromatography kit, kit provided by the invention is convenient to use, to reagent strip can be rapidly secured in box body；
Box body and box cover easy disassembly, and it is avoided that foreign matter is fallen on reagent strip.
(2) technical solution
To solve the above problems, the present invention provides a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed to chromatograph examination
Agent box, including box body, reagent strip, box cover, the first sealing-plug, the second sealing-plug, catheter and tightening for fixating reagent item
Component；Wherein, tightening component includes stripper plate, tightening piece and sliding part；
Box body is equipped with opening, and the peripheral end face of the opening of box body is equipped with flange plate；Place reagent strip in the bottom of box body；Reagent
The end face of item towards stripper plate compresses stripper plate respectively；Stripper plate is separately positioned on sliding part；Sliding part is slidably connected respectively
The inner wall of box body is respectively equipped with the first mounting hole on sliding part；Tightening piece is threaded into respectively in the first mounting hole, and tightening piece
The end face for being respectively facing box body compresses box body respectively；
Box cover is equipped with the liquid feeding groove for sample to be tested to be added dropwise to reagent strip and the observation groove for observing reagent strip；
The inner face of box cover is equipped with groove, and box cover fastens the opening that box body is arranged in；Flange plate is located in groove；
The bottom of liquid feeding groove is respectively equipped with the second mounting hole；The inside of liquid feeding groove, the second mounting hole and box body is sequentially communicated；
One end of catheter is threaded into respectively in the second mounting hole, and the other end of catheter is located at the upper of the sample application zone of reagent strip
Side；Second sealing-plug is detachably arranged in liquid feeding groove；
The bottom of observation groove is respectively equipped with third mounting hole；The inside of observation groove, third mounting hole and box body is sequentially communicated；
First sealing-plug is detachably arranged in observation groove.
Preferably, elastic cushion is respectively equipped on the end face of stripper plate and reagent strip fitting.
Preferably, flange plate is end to end is arranged on the outer end face of box body, and the one end of flange plate far from box body is ball
Face.
Preferably, catheter is towards being respectively equipped with back taper liquid outlet in one end nozzle of reagent strip.
Preferably, the first sealing-plug is made of rubber material, and the first sealing-plug is equipped with the first handle.
Preferably, the first sealing-plug includes the first sealing and the second sealing；First sealing connects the second sealing,
And first sealing and the second sealing be structure as a whole；First sealing is rectangular parallelepiped structure, the first sealing interference setting
In observation groove；Second sealing position rectangular parallelepiped structure, the second sealing position is in third mounting hole, the cross of the second sealing
Sectional area is not more than the cross-sectional area of the first sealing.
Preferably, the second sealing-plug selection is made of rubber material, and the second sealing-plug is equipped with the second handle.
Preferably, for the length value of the second sealing-plug not less than the depth value of pressurization groove, the second sealing-plug includes that third is close
Envelope portion and the 4th sealing；Third sealing and the connection of the 4th sealing, and third sealing and the 4th sealing are integrated knot
Structure；Third sealing is cylindrical structure, and third sealing interference is arranged in liquid feeding groove；4th sealing is truncated cone-shaped knot
Structure, the 4th sealing are identical as the diameter of the diameter of third sealing connecting end surface and third sealing.
Preferably, there are gaps between reagent strip and the surrounding side wall of box body.
Preferably, reagent strip includes substrate, sample pad, bonding pad, nitrocellulose filter and water absorption pad；Nitrocellulose filter
It is arranged on the end face of substrate, nitrocellulose filter is equipped with detection T line and Quality Control C line；Bonding pad and water absorption pad difference
The two sides of substrate are set, and bonding pad and water absorption pad compress nitrocellulose filter respectively；Sample pad is overlapped on bonding pad respectively
On, and sample pad is located at the underface of liquid feeding groove.
Above-mentioned technical proposal of the invention has following beneficial technical effect:
In use, reagent strip is placed in box body, sliding part is mobile towards the side of reagent strip；Stripper plate is compressed respectively
Reagent strip rotates tightening piece, the end face of tightening piece towards box body is compressed box body respectively, so that reagent strip is fixedly mounted on box
In vivo, also facilitate and reagent strip is dismantled；
It opens the second sealing-plug test specimens to be checked are added in liquid feeding groove, detects sample successively from the second mounting hole, drain
The inside of pipe is added drop-wise in the sample pad of reagent strip；Sample is added dropwise by the catheter of setting to avoid dropper extending into box body
It is interior, to avoid bringing foreign matter into box body；
After being added dropwise to complete, the second sealing-plug is being sealed in pressurization groove；First sealing-plug is taken out in groove from, is led to
It crosses observation groove and the second mounting hole facilitates observation reagent strip；
Box body is connected by the way of fastening with box cover, and box cover is mounted on box body to facilitate, and box body and box cover are repeatable
It utilizes, greatly improves its availability.
Detailed description of the invention
Fig. 1 is that a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed proposed by the present invention chromatographs kit
Structural schematic diagram.
Fig. 2 is that a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed proposed by the present invention chromatographs in kit
The structural schematic diagram of partial enlargement at A.
Fig. 3 is that a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed proposed by the present invention chromatographs in kit
The structural schematic diagram of partial enlargement at B.
Fig. 4 is that a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed proposed by the present invention chromatographs in kit
The structural schematic diagram of partial enlargement at C.
Fig. 5 is that a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed proposed by the present invention chromatographs kit
Top view.
Fig. 6 is that a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed proposed by the present invention chromatographs in kit
The schematic perspective view of first sealing-plug.
Fig. 7 is that a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed proposed by the present invention chromatographs in kit
The schematic perspective view of second sealing-plug.
Fig. 8 is that a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed proposed by the present invention chromatographs in kit
The schematic perspective view of reagent strip.
Appended drawing reference: 1, box body；2, reagent strip；3, box cover；4, the first sealing-plug；41, the first sealing；42, second is close
Envelope portion；5, the second sealing-plug；51, third sealing；52, the 4th sealing；6, catheter；61, back taper liquid outlet；7, flange
Plate；8, stripper plate；9, tightening piece；10, sliding part；11, elastic cushion；21, substrate；22, sample pad；23, bonding pad；24, nitric acid
Cellulose membrane；25, T line is detected；26, Quality Control C line；27, water absorption pad.
Specific embodiment
In order to make the objectives, technical solutions and advantages of the present invention clearer, With reference to embodiment and join
According to attached drawing, the present invention is described in more detail.It should be understood that these descriptions are merely illustrative, and it is not intended to limit this hair
Bright range.In addition, in the following description, descriptions of well-known structures and technologies are omitted, to avoid this is unnecessarily obscured
The concept of invention.
As shown in figures 1-8, a kind of time-resolved fluoroimmunoassay chromatography detecting Mycotoxins in Feed proposed by the present invention
Kit, including box body 1, reagent strip 2, box cover 3, the first sealing-plug 4, the second sealing-plug 5, catheter 6 and be used for fixating reagent
The tightening component of item 2；Wherein, tightening component includes stripper plate 8, tightening piece 9 and sliding part 10；
Box body 1 is equipped with opening, and the peripheral end face of the opening of box body 1 is equipped with flange plate 7；Place reagent strip in the bottom of box body 1
2；Reagent strip 2 compresses stripper plate 8 towards the end face of stripper plate 8 respectively；Stripper plate 8 is separately positioned on sliding part 10；Sliding part
10 inner walls for being slidably connected box body 1 respectively are respectively equipped with the first mounting hole on sliding part 10；Tightening piece 9 is threaded into respectively
In one mounting hole, and tightening piece 9 is respectively facing the end face of box body 1 and compresses box body 1 respectively；
It should be noted that box body 1 is made of plastic material；Sliding slot is equipped on the inner wall of box body 1；It is slidably installed in sliding slot
Sliding part 10；Tightening piece 9 compresses the bottom surface of sliding slot respectively；
Box cover 3 is equipped with the liquid feeding groove for sample to be tested to be added dropwise to reagent strip 2 and the observation for observing reagent strip 2 is recessed
Slot；The inner face of box cover 3 is equipped with groove；When box cover 3 and box body 1 connect, box cover 3 is fastened to the opening that box body 1 is arranged in, it is convex
Listrium 7 is located in groove, and box cover 3 compresses box body 1 towards the end face of box body 1 respectively；
It should be noted that box cover 3 is made of transparent plastics material；Box cover 3 is connect by way of fastening with box body 1, side
Just box cover 3 and box body 1 are dismounted, facilitates and box body 1 is placed or taken out to reagent strip 2, to realize box body 1 and box cover 3
Recoverable, improve its availability；
The bottom of liquid feeding groove is respectively equipped with the second mounting hole；The inside of liquid feeding groove, the second mounting hole and box body 1 successively connects
It is logical；One end of catheter 6 is threaded into respectively in the second mounting hole, and the other end of catheter 6 is located at the sample-adding of reagent strip 2
The top in area；Test specimens to be checked are added in liquid feeding groove, and detection sample is successively dripped from the inside of the second mounting hole, catheter 6
It is added in the sample pad 22 of reagent strip 2；Sample is added dropwise by the catheter 6 of setting to avoid for dropper extending into box body 1, thus
Band people's impurity is avoided to enter in box body 1；
Further, liquid feeding groove is multiple along the width direction spaced set of box cover 3；It avoids only being separately provided one big
Liquid filling hole, increase foreign matter equipped with liquid filling hole and fall to the probability in box body 1, can be reduced foreign matter equipped with multiple liquid feeding grooves
The probability of entrance, and the setting of liquid feeding groove is multiple, equidistantly can carry out that test specimens to be checked are added dropwise in the upper of sample pad 22；
Second sealing-plug 5 is detachably arranged in liquid feeding groove；By the second sealing-plug 5 of setting by liquid feeding groove seal plug
Firmly, it is fallen into liquid feeding groove to avoid foreign matter when detection；
The bottom of observation groove is respectively equipped with third mounting hole；The inside of observation groove, third mounting hole and box body 1 successively connects
It is logical；First sealing-plug 4 is detachably arranged in observation groove, will be observed groove sealing by the first sealing-plug 4 of setting and is blocked,
It is fallen into observation groove to avoid foreign matter when detection.
In an alternative embodiment, elastic cushion 11 is respectively equipped on the end face that stripper plate 8 and reagent strip 2 are bonded；It is logical
The elastic cushion 11 for crossing setting avoids stripper plate 8 from damaging reagent strip 2.
In an alternative embodiment, on the end to end outer end face that box body 1 is arranged in of flange plate 7, flange plate 7 is remote
One end from box body 1 is spherical surface.
In an alternative embodiment, catheter 6 goes out liquid towards being respectively equipped with back taper in one end nozzle of reagent strip 2
Mouth 61；Liquid can slow down falling speed when passing through back taper liquid outlet 61, avoid liquid from falling too urgent too fast.
In an alternative embodiment, the first sealing-plug 4 is made of rubber material, and the first sealing-plug 4 is equipped with
First handle；
In an alternative embodiment, the first sealing-plug 4 includes the first sealing 41 and the second sealing 42；First sealing
41 the second sealings 42 of connection, and the first sealing 41 and the second sealing 42 are structure as a whole；
First sealing 41 is rectangular parallelepiped structure, and 41 interference of the first sealing setting is in observation groove；
Second sealing, 42 bit length cube structure, 42 interference of the second sealing are arranged in third mounting hole, the second sealing 42
Cross-sectional area be not more than the first sealing 41 cross-sectional area.
In an alternative embodiment, the second sealing-plug 5 selection is made of rubber material, and on the second sealing-plug 5
Equipped with the second handle.
It should be noted that the first handle and the second handgrips are identical, link is selected respectively；Link is rubber material system
At user pinches suspension loop preventing with hand to install or take sealing-plug.
In an alternative embodiment, for the length value of the second sealing-plug 5 not less than the depth value of pressurization groove, second is close
Blocking 5 includes third sealing 51 and the 4th sealing 52；Third sealing 51 and the connection of the 4th sealing 52, and third seals
Portion 51 and the 4th sealing 52 are structure as a whole；
Third sealing 51 is cylindrical structure, and 51 interference of third sealing is arranged in liquid feeding groove；
4th sealing 52 is truncated cone-shaped structure, and the diameter and third of the 4th sealing 52 and 51 connecting end surface of third sealing are close
The diameter in envelope portion 51 is identical.
In an alternative embodiment, there are gaps between reagent strip 2 and the surrounding side wall of box body 1.
In an alternative embodiment, reagent strip 2 includes substrate 21, sample pad 22, bonding pad 23, nitrocellulose filter
24 and water absorption pad 27；Nitrocellulose filter 24 is arranged on the end face of substrate 21, and nitrocellulose filter 24 is equipped with detection T
Line 25 and Quality Control C line 26；Substrate 21 uses PVC plate；The aflatoxin AFB1 antigen embedded on detection T line 25；Quality Control C line
Two antiantibody of goat-anti rabbit embedded on 26；
Bonding pad 23 and water absorption pad 27 are separately positioned on the two sides of substrate 21, and bonding pad 23 and water absorption pad 27 compress nitric acid respectively
Cellulose membrane 24；Sample pad 22 is overlapped on respectively on bonding pad 23, and sample pad 22 is located at the underface of liquid feeding groove.
In the present invention, in use, reagent strip 2 is placed in box body 1, by sliding part 10 towards a sidesway of reagent strip 2
It is dynamic；Stripper plate 8 is compressed into reagent strip 2 respectively, tightening piece 9 is rotated, the end face of tightening piece 9 towards box body 1 is compressed into box body respectively
1, so that reagent strip 2 is fixedly mounted in box body 1, also facilitates and reagent strip 2 is dismantled；Opening the second sealing-plug 5 will be to
Detection sample is added in liquid feeding groove, and detection sample is successively added drop-wise to reagent strip 2 from the inside of the second mounting hole, catheter 6
Sample pad 22 on；Sample is added dropwise by the catheter 6 of setting to avoid for dropper extending into box body 1, thus avoid impurity into
Enter in box body 1；After being added dropwise to complete, the second sealing-plug 5 is being sealed in pressurization groove；By the first groove from of sealing-plug 4
Interior taking-up facilitates observation reagent strip 2 by observation groove and the second mounting hole.
It should be understood that above-mentioned specific embodiment of the invention is used only for exemplary illustration or explains of the invention
Principle, but not to limit the present invention.Therefore, that is done without departing from the spirit and scope of the present invention is any
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.In addition, appended claims purport of the present invention
Covering the whole variations fallen into attached claim scope and boundary or this range and the equivalent form on boundary and is repairing
Change example.
Claims (10)
1. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed chromatographs kit, which is characterized in that including box body
(1), reagent strip (2), box cover (3), the first sealing-plug (4), the second sealing-plug (5), catheter (6) and be used for fixating reagent item
(2) tightening component；Wherein, tightening component includes stripper plate (8), tightening piece (9) and sliding part (10)；
Box body (1) is equipped with opening, and the peripheral end face of the opening of box body (1) is equipped with flange plate (7)；It puts the bottom of box body (1)
Set reagent strip (2)；The end face of reagent strip (2) towards stripper plate (8) compresses stripper plate (8) respectively；Stripper plate (8) is separately positioned on
On sliding part (10)；Sliding part (10) is slidably connected the inner wall of box body (1) respectively, and sliding part is respectively equipped with the first installation on (10)
Hole；Tightening piece (9) is threaded into respectively in the first mounting hole, and tightening piece (9) is respectively facing the end face of box body (1) and compresses respectively
Box body (1)；
Box cover (3) is equipped with for the liquid feeding groove of sample to be tested to be added dropwise to reagent strip (2) and for observing reagent strip (2)
Observe groove；The inner face of box cover (3) is equipped with groove, and box cover (3) fastens setting in the opening of box body (1)；Flange plate (7) position
In in groove；
The bottom of liquid feeding groove is respectively equipped with the second mounting hole；The inside of liquid feeding groove, the second mounting hole and box body (1) successively connects
It is logical；One end of catheter (6) is threaded into respectively in the second mounting hole, and the other end of catheter (6) is located at reagent strip (2)
Sample application zone top；Second sealing-plug (5) is detachably arranged in liquid feeding groove；
The bottom of observation groove is respectively equipped with third mounting hole；The inside of observation groove, third mounting hole and box body (1) successively connects
It is logical；First sealing-plug (4) is detachably arranged in observation groove.
2. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 1 chromatographs kit,
It is characterized in that, being respectively equipped with elastic cushion (11) on the end face of stripper plate (8) and reagent strip (2) fitting.
3. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 1 chromatographs kit,
It is arranged on the outer end face of box body (1) it is characterized in that, flange plate (7) is end to end, flange plate (7) is far from box body (1)
One end is spherical surface.
4. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 1 chromatographs kit,
It is characterized in that, catheter (6) is towards being respectively equipped with back taper liquid outlet (61) in one end nozzle of reagent strip (2).
5. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 1 chromatographs kit,
It is characterized in that, the first sealing-plug (4) is made of rubber material, and the first sealing-plug (4) is equipped with the first handle.
6. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 5 chromatographs kit,
It is characterized in that, the first sealing-plug (4) includes the first sealing (41) and the second sealing (42)；First sealing (41) connection
Second sealing (42), and the first sealing (41) and the second sealing (42) are structure as a whole；First sealing (41) is length
Cube structure, the first sealing (41) interference setting is in observation groove；Second sealing (42) bit length cube structure, second is close
Envelope portion (42) is located in third mounting hole, and the cross-sectional area of the second sealing (42) is not more than the cross section of the first sealing (41)
Product.
7. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 1 chromatographs kit,
It is characterized in that, the second sealing-plug (5) selection is made of rubber material, and the second sealing-plug (5) is equipped with the second handle.
8. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 7 chromatographs kit,
It is characterized in that, depth value of the length value of the second sealing-plug (5) not less than pressurization groove, the second sealing-plug (5) includes third
Sealing (51) and the 4th sealing (52)；Third sealing (51) and the connection of the 4th sealing (52), and third sealing
(51) it is structure as a whole with the 4th sealing (52)；Third sealing (51) is cylindrical structure, third sealing (51) interference
It is arranged in liquid feeding groove；4th sealing (52) is truncated cone-shaped structure, and the 4th sealing (52) and third sealing (51) are even
The diameter of contact surface is identical with the diameter of third sealing (51).
9. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 1 chromatographs kit,
It is characterized in that, between reagent strip (2) and the surrounding side wall of box body (1), there are gaps.
10. a kind of time-resolved fluoroimmunoassay for detecting Mycotoxins in Feed according to claim 9 chromatographs kit,
It is characterized in that, reagent strip (2) includes substrate (21), sample pad (22), bonding pad (23), nitrocellulose filter (24) and water suction
It pads (27)；Nitrocellulose filter (24) is arranged on the end face of substrate (21), and nitrocellulose filter (24) is equipped with detection T
Line (25) and Quality Control C line (26)；Bonding pad (23) and water absorption pad (27) are separately positioned on the two sides of substrate (21), and bonding pad
(23) and water absorption pad (27) compresses nitrocellulose filter (24) respectively；Sample pad (22) is overlapped on respectively on bonding pad (23), and
Sample pad (22) is located at the underface of liquid feeding groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910499683.2A CN110133264A (en) | 2019-06-11 | 2019-06-11 | A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed |
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|---|---|---|---|
| CN201910499683.2A CN110133264A (en) | 2019-06-11 | 2019-06-11 | A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110133264A true CN110133264A (en) | 2019-08-16 |
Family
ID=67580961
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|---|---|---|---|
| CN201910499683.2A Pending CN110133264A (en) | 2019-06-11 | 2019-06-11 | A kind of time-resolved fluoroimmunoassay chromatography kit detecting Mycotoxins in Feed |
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| CN (1) | CN110133264A (en) |
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