CN205216828U - Parallel screening active material's of multichannel device - Google Patents
Parallel screening active material's of multichannel device Download PDFInfo
- Publication number
- CN205216828U CN205216828U CN201520850946.7U CN201520850946U CN205216828U CN 205216828 U CN205216828 U CN 205216828U CN 201520850946 U CN201520850946 U CN 201520850946U CN 205216828 U CN205216828 U CN 205216828U
- Authority
- CN
- China
- Prior art keywords
- bar magnet
- sleeve pipe
- row
- test tube
- elevating mechanism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The utility model discloses a parallel screening active material's of multichannel device, including quick -witted case, the bottom of quick -witted case is equipped with the mount of fixed multirow test tube, the bar magnet of one row of liftable and the bottom confined sleeve pipe of one row of liftable are established to the mount top, the sleeve pipe is located between mount and the bar magnet, bar magnet, sleeve pipe and same row's test tube from last one -to -one setting extremely down, bar magnet and sleeve pipe decline in -process, bar magnet can stretch into sleeve pipe and in vitro respectively, still be equipped with drive bar magnet and the sleeve pipe translation mechanism along perpendicular its array orientation in the box, the device is still including the control mechanism who controls the motion of bar magnet and sleeve pipe. This device utilizes the bar magnet to carry out the magnetic bead and in the transfer of different test tubes, realizes the automation mechanized operation of multistep reaction, avoids the operate miss, the design of multichannel realizes going on when the multiunit screening is operated, improves the screening efficiency.
Description
Technical field
The utility model relates to active material Rapid screening techniques field, is specifically related to a kind of device of multi-channel parallel screening active substances.
Background technology
Pharmaceutically active substance screening study is significant in new drug development design, toxicologic study etc.Target affinity selection method is the method that a class set becomes analytical instrument and drug targets, utilizes the affinity interaction of active material and target, deposition activity material.Based on these general principles, also there is the quick screening application for different pharmaceutical, such as medicaments sifting chip.Notification number is that the patent document of CN1996014B discloses a kind of array micro-fluidic chip device for drug metabolism screening, and comprise sample introduction interface and micro-fluidic chip, wherein sample introduction interface is made up of upper cover plate and lower cover slip, is provided with multiple sample introduction siphunculus in upper cover plate; Micro-fluidic chip system is one-body molded, one side is provided with multiple liquid storage and enters pond, the other end is provided with multiple liquid storage and goes out pond, multiple microchannel is also provided with in this micro-fluidic chip, be embedded with activated protein in it or contain active proteic substance, one end and this liquid storage of this microchannel enter one end, pond and are connected, and the other end and this liquid storage go out one end, pond and be connected, this micro-fluidic chip is installed with between the upper and lower cover plate of sample introduction interface, and the inner of this sample introduction siphunculus and this liquid storage are entered, and the other end in pond is corresponding communicates.This contrive equipment is easy to make, simple to operate, good stability and analysis throughput is high.
Immuno magnetic cell separation technology is a kind of new immunological technique combined by distinctive to the high degree of specificity of immunological response and magnetic bead magnetic responsiveness, is also that recent year popularizes use and studied more a kind of immunological technique.Immunomagnetic beads can combine with the specific antibody be connected on magnetic bead (or antigen) based on antigen (or antibody).In outside magnetic field, the antigen antibody complex be connected with magnetic bead is trapped in magnetic field by adsorbing, and other compositions be not connected with magnetic bead of reaction system are not owing to having magnetic, does not stop in magnetic field, thus antigen antibody complex is separated.The method have simple, specificity is high, it is little to lose, the advantage that also immune separation and consentration can be combined as a whole.In 500 known at present multi-medicament action targets, enzyme is a most important class.Utilize surface-functionalized magnetic bead, nano material etc. as the carrier of immobilized enzyme, the activity of firm enzyme, is conducive to the operation that outside realizes enrichment, separation on the other hand on the one hand.The existing magnetic bead separating device being applied to 96 hole microwell plates, most employing imposes magnetic field and is separated magnetic bead bottom microwell plate.But the interpolation of solution in each step (sieving sample, cleaning fluid, dissociation solution etc. as waited) and drawing completes by manual operation substantially, when needing to screen a large amount of sample, this workload is very huge.Manual operation also Problems existing be operate miss and operation time open environment on the impact of active material stability.Because the floor space of microwell plate is less, with bottom surface magnetic fields limited area, so effect is poor in Beads enrichment.
It is low to there is screening effeciency in existing drug screening device platform.In order to raise the efficiency further, screen while seeking to realize Mutiple Targets, the utility model adopts multi-channel parallel filtering mode, natural extracts is flowed through the passage of different target configuration simultaneously, obtains multiple active material.
Utility model content
The utility model provides a kind of device of multi-channel parallel screening active substances, realizes the object of high flux screening bioactive compounds, overcomes the inefficient problem of conventional medicament filtering mode.
A kind of device of multi-channel parallel screening active substances, comprise cabinet, the bottom of cabinet is provided with the fixed mount of fixing many row's test tubes, the sleeve pipe of the bar magnet establishing a row liftable above described fixed mount and the liftable bottom end closure of a row, described sleeve pipe is between fixed mount and bar magnet, described bar magnet, the test tube one_to_one corresponding setting from top to bottom of sleeve pipe and same row, in bar magnet and sleeve pipe decline process, bar magnet can stretch into sleeve pipe and in vitro respectively, also be provided with in described casing and drive bar magnet and the translation mechanism of sleeve pipe along its orientation vertical, described device also comprises the controlling organization controlling bar magnet and sleeve movement.
The utility model utilizes attracting each other of bar magnet and magnetic bead, drives bar magnet to move, realizes the transfer of magnetic bead between each row's test tube, carry out the operation of multi-step, realize separation and the enrichment of active material.Basic procedure is: 1, extract magnetic bead: bar magnet stretches in sleeve pipe, and entirety stretches into and is equipped with in the first row test tube of the magnetic bead combining target downwards, and absorption magnetic bead, rises, move to above next test tube; 2, hatch: bar magnet and sleeve pipe entirety stretch in the second row test tube being equipped with and waiting to sieve sample, and bar magnet rises, magnetic bead is dispersed in be treated in sieve sample; 3, the operation of 1 is repeated; 4, clean: bar magnet and sleeve pipe entirety stretch into and is equipped with in the 3rd row's test tube of cleaning fluid, and bar magnet rises, and magnetic bead is dispersed in cleaning fluid; 5, the operation of 1 is repeated; 6, wash-out: bar magnet and sleeve pipe entirety stretch into and is equipped with in the 4th row's test tube of eluent, and bar magnet rises, and magnetic bead is dispersed in eluent; 7, magnetic bead is reclaimed.Containing screening the active material obtained in eluent.Wait to sieve by adjusting in each test tube of same row the strategy that target that in the kind of sample or each test tube of same row, magnetic bead combines can realize multi-channel parallel screening.
PLC chip selected by described controlling organization, is mainly used in the setting of parameters, and controling parameters comprises the duration of oscillation of stand-by period above test tube of bar magnet and sleeve pipe and sleeve pipe, oscillation amplitude and frequency of oscillation.
All bar magnet tops are fixed on first connecting rod, the first elevating mechanism is provided with above first connecting rod, described cannula tip is fixed on second connecting rod, is provided with the second elevating mechanism above second connecting rod, and the first elevating mechanism and the second elevating mechanism are fixedly connected on translation mechanism.
First elevating mechanism and the second elevating mechanism control the vertical displacement movement of bar magnet and sleeve pipe respectively.First elevating mechanism and the second elevating mechanism are fixedly connected on translation mechanism, and along with the first elevating mechanism and the second elevating mechanism are along the movement of translation mechanism, bar magnet and sleeve pipe simultaneously occurred level move.
Preferred version, described first elevating mechanism and the second elevating mechanism are cylinder, and described translation mechanism is for relying on motor-driven leading screw, and cylinder is fixedly connected on the nut of leading screw.
First elevating mechanism and the second elevating mechanism are respectively using cylinder as power source, elevating mechanism is made up of two symmetrical cylinders, the piston rod of two cylinders of the first elevating mechanism is fixedly connected with head rod, the piston rod of two cylinders of the second elevating mechanism connects the second connecting rod, realizes the different heave amplitude of elevating mechanism by regulating the amount of gas pressure passing into cylinder.Described translation mechanism is for relying on motor-driven leading screw, and when motor drives the bolt rotary on leading screw, nut moves horizontally on screw rod, and the cylinder be connected with nut also moves thereupon.More firm in order to connect, connected by gusset piece between cylinder and nut.As preferably, motor is servomotor, and the output accuracy of servomotor is high, can the shift position of more accurately control cylinder.
Described leading screw is fixed on the inner chamber end face of cabinet.The inner chamber end face of cabinet is provided with a pair line slideway, and leading screw is arranged between two line slideways.
Described test tube is at least 4 rows.Complete above-mentioned basic procedure, 4 rows are at least arranged to by test tube, respectively add in conjunction with target magnetic bead liquid, wait sieve sample, cleaning fluid and eluent.Generally, the step of cleaning needs repetition three times, therefore the test tube adding cleaning fluid can be increased to 3 rows.
Preferred scheme, described fixed mount is placed in heating water bath case.Fixed mount is placed in heating water bath case, is controlled the temperature of in vitro liquid by the temperature controlling water-bath, thus ensures the stability treating sieve active material.
More preferred, be provided with dividing plate in described heating water bath case, its inner chamber is separated into some cells by dividing plate, and the test tube of same row is placed in same little indoor, and the test tube of different row is in the little indoor of difference.Heating water bath box cavity is separated into some cells by dividing plate, and each cell arranges heater respectively.According to the temperature requirement of concrete reactions steps, control the bath temperature of each cell respectively, while guarantee active material stability, the reaction making each walk is more thorough.
Preferred scheme, device of the present utility model also comprises sample charging mechanism and fluid reservoir.Device realizes automatic sample, not only improves precision and the speed of application of sample, also saves artificial and time cost, is applicable to large batch of screening substances.Sample charging mechanism connects fluid reservoir, controls sample introduction by negative pressure pump.Test tube for each row arranges a sample charging mechanism and fluid reservoir, realizes the interpolation of different solutions.Described sample charging mechanism comprises sample injector and manipulator.The movement of sample injector is realized by manipulator, manipulator comprises elevating mechanism and translation mechanism, translation mechanism is arranged on cabinet inner chamber end face, elevating mechanism is fixed on the sliding shoe of translation mechanism, sample injector is fixedly connected on elevating mechanism, moved to above corresponding test tube by sample injector by translation mechanism, sample injector is down to test tube internal orifice place by elevating mechanism, and liquid in fluid reservoir pumps in vitro by negative pressure pump effect.
Compared to prior art, the beneficial effect that the utility model possesses: (1) the utility model utilizes bar magnet to carry out the transfer of magnetic bead at different test tube, realizes the automation mechanized operation of multistep reaction, avoids operate miss; (2) multichannel design realizes carrying out while many groups screen operation, improve screening effect, and the applicability of this device is extensive, may be used on multiple technical fields such as bio-separation, immunity separation and drug screening.
Accompanying drawing explanation
Fig. 1 is the front schematic view of the device of the utility model multi-channel parallel screening active substances.
Fig. 2 is the side schematic view of the device of the utility model multi-channel parallel screening active substances.
Detailed description of the invention
Below in conjunction with accompanying drawing, preferred embodiment of the present utility model is described in detail, makes advantage of the present utility model and feature can be easier to be readily appreciated by one skilled in the art, thus more explicit defining is made to protection domain of the present utility model.
As shown in Figure 1, the device of the utility model multi-channel parallel screening active substances, comprises cabinet 1, and the bottom of cabinet is provided with the fixed mount (not indicating in figure) of fixing many row's test tubes 4.As shown in Figure 2, test tube is set to 6 rows.Fixed mount is placed in heating water bath case 14, is provided with dividing plate 141 in heating water bath case 14, and its inner chamber is separated into some cells by dividing plate 141, and the test tube of same row is placed in same little indoor, and the test tube of different row is in the little indoor of difference.Each cell arranges heater (not indicating in figure) respectively, and heater is connected with controlling organization 11, and user can arrange the temperature parameter of controlling organization as the case may be thus adjust the bath temperature of each cell respectively.
It is symmetrical that cabinet 1 inner chamber end face is provided with a pair line slideway, 71, two line slideways 71, and line slideway 71 two ends are fixed by holder 72.Article two, be provided with leading screw between line slideway, the two ends of the screw rod 8 of leading screw are connected on holder 73, and one end of screw rod 8 is connected by shaft coupling with the output shaft of servomotor 9, and the output shaft of servomotor rotarily drives screw rod 8 and rotates.The nut of leading screw is provided with gusset piece 10, and two line slideways 71 are arranged in the two ends of gusset piece 10 respectively, and when the screw rod 8 of leading screw rotates, nut can move horizontally on screw rod, drives gusset piece 10 to move along line slideway 71.
The bottom surface of gusset piece 10 be arranged in parallel the first elevating mechanism and the second elevating mechanism, and the first elevating mechanism is made up of two symmetrical cylinders 51,52, and the second elevating mechanism is made up of two symmetrical cylinders 61,62.The piston rod 511,521 of cylinder 51,52 connects row's bar magnet 2 by head rod 21, and the piston rod 611,621 of cylinder 61,62 connects a bar casing tube 3 by the second connecting rod 31.Sleeve pipe 3 is between bar magnet 2 and test tube 4, and the test tube 4 of bar magnet 2, sleeve pipe 3 and same row from top to bottom one_to_one corresponding is arranged, and bar magnet 2 and sleeve pipe 3 are in decline process, and bar magnet 2 can stretch in sleeve pipe 3 and test tube 4 respectively.Bar magnet 2 adopts the permanent magnet of column structure, and sleeve pipe 3 is plastics, bottom end closure, and inner tubal wall matches with bar magnet 2.When the screw rod 8 of leading screw rotates, the cylinder be connected with gusset piece 10, bar magnet and sleeve pipe also move thereupon, and servomotor accurately controls the shift position of bar magnet and sleeve pipe.When bar magnet and sleeve pipe are positioned at above target test tube, the air pressure in control cylinder realizes the lifting of bar magnet and sleeve pipe.The operation of the first elevating mechanism and the second elevating mechanism is controlled by controlling organization 11, and controlling organization 11 can select traditional PLC chip, with the automation mechanized operation of implement device.
Device of the present utility model also comprises sample charging mechanism and fluid reservoir 13.Sample charging mechanism connects fluid reservoir, controls sample introduction by negative pressure pump (not indicating in figure).The controlled mechanism 11 of negative pressure pump controls, and controlling organization 11 realizes application of sample optimum configurations.Test tube for each row arranges a sample charging mechanism and fluid reservoir, realizes the interpolation of different solutions.Described sample charging mechanism comprises sample injector 12 and manipulator (not indicating in figure).The movement of sample injector is realized by manipulator, manipulator comprises elevating mechanism and travel mechanism, travel mechanism is arranged on cabinet inner chamber end face, elevating mechanism is fixed on the sliding shoe of translation mechanism, sample injector is fixedly connected on elevating mechanism, moved to above corresponding test tube by sample injector by travel mechanism, sample injector is down to test tube internal orifice place by elevating mechanism, and liquid in fluid reservoir pumps in vitro by negative pressure pump effect.
For enzyme target affinity selection method, the operating process of the utility model device is described: (1) application of sample: as shown in Figure 2, from left to right successively toward in vitro adding immobilised enzymes magnetic bead liquid, waiting to sieve sample, cleaning fluid 1, cleaning fluid 2, cleaning fluid 3 and dissociation solution; (2) extract magnetic bead: sleeve pipe stretches in first row test tube downwards, up and down vibration mixing magnetic bead, bar magnet stretches into downwards in sleeve pipe, absorption magnetic bead, and entirety rises, and moves to above next test tube; (3) hatch: bar magnet and sleeve pipe entirety stretch in second row test tube downwards, bar magnet rises, and magnetic bead is distributed to be treated in sieve sample solution, sleeve pipe vibrates mixing up and down, hatches certain hour, catches active material by target, bar magnet decline is subsequently stretched in sleeve pipe, carries out the same absorption migration; (4) clean: magnetic rod sleeve entirety stretches into downwards in the 3rd row's test tube, and bar magnet rises, and magnetic bead is distributed in cleaning fluid 1, and sleeve pipe vibrates mixing up and down, and absorption migration is carried out in bar magnet decline subsequently, and this step repeats twice again; (5) wash-out: magnetic bead is transferred to and is equipped with in the test tube of dissociation solution, and sleeve pipe vibrates up and down, bar magnet decline absorption magnetic bead, overall rise, moves to first row test tube and reclaims, and gets dissociation solution and does analysis detection.
The foregoing is only embodiment of the present utility model, not thereby limit the scope of the claims of the present utility model, every equivalent structure transformation utilizing the utility model description and accompanying drawing content to do, all in scope of patent protection of the present utility model.
Claims (9)
1. the device of a multi-channel parallel screening active substances, it is characterized in that, comprise cabinet, the bottom of cabinet is provided with the fixed mount of fixing many row's test tubes, the sleeve pipe of the bar magnet establishing a row liftable above described fixed mount and the liftable bottom end closure of a row, described sleeve pipe is between fixed mount and bar magnet, described bar magnet, the test tube one_to_one corresponding setting from top to bottom of sleeve pipe and same row, in bar magnet and sleeve pipe decline process, bar magnet can stretch into sleeve pipe and in vitro respectively, also be provided with in described casing and drive bar magnet and the translation mechanism of sleeve pipe along its orientation vertical, described device also comprises the controlling organization controlling bar magnet and sleeve movement.
2. device as claimed in claim 1, it is characterized in that, all bar magnet tops are fixed on first connecting rod, the first elevating mechanism is provided with above first connecting rod, described cannula tip is fixed on second connecting rod, be provided with the second elevating mechanism above second connecting rod, the first elevating mechanism and the second elevating mechanism are fixedly connected on translation mechanism.
3. device as claimed in claim 2, it is characterized in that, described first elevating mechanism and the second elevating mechanism are cylinder, and described translation mechanism is for relying on motor-driven leading screw, and cylinder is fixedly connected on the nut of leading screw.
4. device as claimed in claim 3, it is characterized in that, described leading screw is fixed on the inner chamber end face of cabinet.
5. device as claimed in claim 1, is characterized in that, described test tube is at least 4 rows.
6. device as claimed in claim 1, it is characterized in that, described fixed mount is placed in heating water bath case.
7. device as claimed in claim 6, it is characterized in that, be provided with dividing plate in described heating water bath case, its inner chamber is separated into some cells by dividing plate, and the test tube of same row is placed in same little indoor, and the test tube of different row is in the little indoor of difference.
8. device as claimed in claim 1, is characterized in that, comprise sample charging mechanism and fluid reservoir.
9. device as claimed in claim 8, it is characterized in that, described sample charging mechanism comprises sample injector and manipulator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520850946.7U CN205216828U (en) | 2015-10-30 | 2015-10-30 | Parallel screening active material's of multichannel device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520850946.7U CN205216828U (en) | 2015-10-30 | 2015-10-30 | Parallel screening active material's of multichannel device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205216828U true CN205216828U (en) | 2016-05-11 |
Family
ID=55891924
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520850946.7U Expired - Fee Related CN205216828U (en) | 2015-10-30 | 2015-10-30 | Parallel screening active material's of multichannel device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205216828U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108801752A (en) * | 2018-08-02 | 2018-11-13 | 佛山科学技术学院 | A kind of sample loading attachment and sample driving device |
| CN109060783A (en) * | 2018-10-25 | 2018-12-21 | 成都博奥新景医学科技有限公司 | A kind of single part chemical luminous immune detection method and system based on bar magnet method |
-
2015
- 2015-10-30 CN CN201520850946.7U patent/CN205216828U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108801752A (en) * | 2018-08-02 | 2018-11-13 | 佛山科学技术学院 | A kind of sample loading attachment and sample driving device |
| CN108801752B (en) * | 2018-08-02 | 2023-11-28 | 佛山科学技术学院 | Sample loading device and sample driving device |
| CN109060783A (en) * | 2018-10-25 | 2018-12-21 | 成都博奥新景医学科技有限公司 | A kind of single part chemical luminous immune detection method and system based on bar magnet method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8506797B2 (en) | Downstream bioprocessing device | |
| CN103602582B (en) | Automatic plasmid-extracting device | |
| CN109055216B (en) | High-throughput 3D cell, tissue-like and organ-like dynamic culture system | |
| JP6026539B2 (en) | Single container manufacturing of biological products | |
| TW201920230A (en) | Integrated continuous manufacturing of therapeutic protein drug substances | |
| CN103756898A (en) | Three-dimensional stress cell culture device capable of applying dynamic load | |
| CN103861735B (en) | Magnetic separation device | |
| CN205590667U (en) | Biological sample preparation system | |
| CN205216828U (en) | Parallel screening active material's of multichannel device | |
| CN107345199A (en) | Biological sample preparation system | |
| CN104117429B (en) | A kind of heating oscillating magnetic flux separator | |
| CN211112037U (en) | High-flux nucleic acid extraction system | |
| CN103923829B (en) | The needle of bar magnet method extraction apparatus and sleeve pipe mobile system are applied with it | |
| US20220136938A1 (en) | Isolation device and isolation method for isolating target particles from liquid samples | |
| TWI829789B (en) | A large-scale magnetic purification system | |
| CN103131632A (en) | Screening method for proteinuria component induced renal interstitial fibrosis based on micro-fluidic chip | |
| CN100370022C (en) | Technology for culturing cell and recovering products, its integrating system thereof | |
| CN206580839U (en) | A kind of enclosed type electric transfers from one department to another system | |
| CN213141893U (en) | Negative-pressure controllable high-efficiency purification platform experimental device | |
| CN217600627U (en) | Full-automatic protein purification system | |
| CN208532766U (en) | A kind of bottom support type magnetic force rack device | |
| CN103694312A (en) | Protein purification chromatographic system | |
| CN210215392U (en) | Cell separation device | |
| CN219333240U (en) | Be used for protein gravity purification device | |
| CN2748466Y (en) | Integral system of cell culturing and product reclaiming |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160511 Termination date: 20181030 |