CN219333240U

CN219333240U - Be used for protein gravity purification device

Info

Publication number: CN219333240U
Application number: CN202223409458.1U
Authority: CN
Inventors: 张帆; 魏迎东; 许飞; 徐文正
Original assignee: Jiangsu Huakang Biotechnology Co ltd
Current assignee: Jiangsu Huakang Biotechnology Co ltd
Priority date: 2022-12-16
Filing date: 2022-12-16
Publication date: 2023-07-14
Anticipated expiration: 2032-12-16

Abstract

The utility model discloses a gravity purification device for protein, which comprises a support, wherein the support is divided into three layers, a collecting frame is arranged on a first layer, a beaker and a test tube are arranged on the collecting frame, a gravity column is arranged on a second layer of the support, three preassembled columns, namely, a preassembled column A, a preassembled column B, a preassembled column C and a preassembled column D, are respectively arranged on a third layer of the support, the support is integrated and made of organic glass, a filter membrane is arranged in the gravity column, scales are marked on the gravity column, the preassembled column A, the preassembled column B, the preassembled column C and the preassembled column D are marked on the gravity column, and the gravity column is connected with the beaker and the test tube below through hoses. The preassembled column is connected with the gravity column below through a hose, and speed controllers are arranged on the hoses; the gravity purification device for the protein has the advantages of being provided with a designed bracket, a matched gravity column, a preassembled column and a collection frame, and solves the problems in the prior art.

Description

Be used for protein gravity purification device

Technical Field

The utility model relates to the technical field of protein purification, in particular to a gravity purification device for protein.

Background

The separation and purification of proteins are widely used in biochemical research applications and are an important operation technique. A typical eukaryotic cell may contain thousands of different proteins, some in very rich quantities, and some containing only a few copies. In order to study a protein, it is necessary to first purify the protein from other proteins and non-protein molecules. The traditional gravity column purification device is obviously not suitable for purifying a large amount of proteins, and the AKTA, SCG and other protein purification instruments can be applied to various chromatographic columns, can observe elution conditions in real time and can adjust the flow rate at any time. However, the protein purification instrument has the advantages of more pipelines, longer preparation and balancing time, higher loss rate if the sample amount is smaller, and low experimental efficiency because only one purification column can be used by one instrument. The traditional gravity column is mainly applied to scientific research, and has the advantages of simple operation, time saving and simultaneous purification of a plurality of or a plurality of proteins compared with a purification instrument.

The application number CN201711448428.2 is a protein purification automatic device suitable for a gravity column, and comprises a bracket, a sample adding component, a gravity column purification component, a receiving component, a second lifting part and a first lifting part, wherein the sample adding component and the gravity column purification component are connected to the bracket in a sliding manner from top to bottom, the receiving component is arranged below the gravity column purification component, and the first lifting part is arranged between the sample adding component and the gravity column purification component and is used for automatically controlling the distance between the sample adding component and the gravity column purification component; the second lifting part is arranged between the guide rail and the gravity column purification assembly or the sample adding assembly and is used for controlling the distance between the sample adding assembly and the receiving assembly. According to the utility model, the distance between the receiving component and the gravity purification component and the distance between the gravity purification component and the sample adding component are required to be regulated by a motor, so that the preparation time before the experiment is increased, the positive production cost of the whole device is increased, one gravity purification component corresponds to only one sample adding component, and after the sample adding component is added with the reagent which cannot be added in sequence, the reagent added later is polluted by the residual of the reagent added earlier, so that the rigor of the experiment is not facilitated.

Disclosure of Invention

The utility model aims to provide a gravity purification device for protein, which has the advantages of a designed bracket, a matched gravity column, preassembled columns and a collection frame, wherein the preassembled columns can improve the experimental rigor, and each pipeline is matched with a speed controller to accurately control the experimental reaction speed, so that the problems in the prior art are solved.

In order to achieve the above purpose, the present utility model provides the following technical solutions: a gravity purification device for protein comprises a support, wherein the support is divided into three layers, a collecting frame is arranged on a first layer, a beaker and a test tube are arranged on the collecting frame, a gravity column is arranged on a second layer of the support, four preassembling columns are arranged on a third layer of the support, and the preassembling columns are respectively a preassembling column A, a preassembling column B, a preassembling column C and a preassembling column D

Preferably, the support is integral and made of plexiglas.

Preferably, a filter membrane is arranged in the gravity column, and scales are marked on the gravity column.

Preferably, the preassembled column A, the preassembled column B, the preassembled column C and the preassembled column D are marked with scales.

Preferably, the gravity column is connected to the beaker and test tube below by a hose. The preassembled column is connected with the gravity column below through a hose.

Preferably, the hoses are provided with speed controllers.

Preferably, the size of the collecting rack is exactly matched with the size of the first layer of the bracket.

The gravity column is made of organic plastic materials, and the preassembled column A, the preassembled column B, the preassembled column C and the preassembled column D are made of organic plastic materials.

The center of the second layer of the bracket is provided with a positioning hole, and the third layer of the bracket is provided with four positioning holes.

Compared with the prior art, the utility model has the following beneficial effects:

this a be used for protein gravity purification device has avoided a large amount of preparation work before the experiment through using the support that designs, through using the speed controller, the speed that control experiment that can be better goes on, through adopting a plurality of pre-assembled post application of sample, the rigor of experiment has been improved, let the experimental result more accurate, through the support of adopting the preparation of organic glass material, let the experimental process more do benefit to the observation, through adopting the partial shipment structure, let the change of each part simpler, cost of maintenance has been reduced, the whole body adopts environmental protection material, the environment has been protected, but reuse after retrieving.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a gravity purification device for proteins according to the present utility model;

FIG. 2 is a schematic front view of a gravity purification device for proteins according to the present utility model;

FIG. 3 is a schematic right-hand view of a gravity purification device for proteins according to the present utility model;

FIG. 4 is a schematic top view of a gravity purification device for proteins according to the present utility model.

The reference numerals in the drawings indicate: 1. a bracket; 2. a collection rack; 3. a beaker; 4. a test tube; 5. a gravity column; 6. preassembling a column A; 7. preassembling a column B; 8. preassembling a column C; 9. preassembling a column D; 10. a hose; 11. a speed controller.

Detailed Description

The technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, but not all embodiments, and all other embodiments obtained by those skilled in the art without making creative efforts based on the embodiments of the present utility model are included in the protection scope of the present utility model.

In the description of the present utility model, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present utility model and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.

Example 1:

referring to FIGS. 1, 2, 3 and 4, a gravity purification device for protein comprises a support 1, wherein the support 1 is divided into three layers, a collection frame 2 is arranged on a first layer, a beaker 3 and a test tube 4 are arranged on the collection frame 2, a gravity column 5 is arranged on a second layer of the support 1, four preassembled columns, namely, a preassembled column A6, a preassembled column B7, a preassembled column C8 and a preassembled column D9, are respectively arranged on a third layer of the support 1, the support 1 is integrated and made of organic glass, a filter membrane is arranged in the gravity column 5, scales are marked on the gravity column 5, the preassembled column A6, the preassembled column B7, the preassembled column C8 and the preassembled column D9 are respectively connected with the lower beaker 3 and the test tube 4 through a hose 10, the preassembled column is connected with the lower gravity column 5 through the hose 10, a speed controller 11 is respectively arranged on the hose 10, the size of the collection frame 2 is exactly matched with the size of the first layer of the support 1, the gravity column 5 is made of organic plastic, the preassembly column A6, the preassembly column B7, the preassembly column C8 and the preassembly column D9 are made of organic plastic, a positioning hole is formed in the center of the second layer of the support 1, and four positioning holes are formed in the third layer of the support 1. Can be reused after being recovered.

Example 2:

referring to fig. 1, 2, 3 and 4, a gravity purification device for protein is shown, wherein a nickel column is firstly arranged in a gravity column 5, plasma water is arranged in a preassembly column A, a sample is arranged in a preassembly column B, a buffer solution is arranged in a preassembly column C, and an eluent is arranged in a preassembly column D before experiments. The preassembled column A6, the preassembled column B7, the preassembled column C8, the preassembled column D9 and the gravity column 5 are placed on the support 1, the collection frame 2 is placed on the support 1, the

preassembled columns

6, 7, 8 and 9 are connected with the gravity column 5 through the hose 10, the gravity column 5 is connected with the beaker 3 and the test tube 4 through the hose 10, and the speed controller 11 between the gravity column 5 and the beaker 3 is opened. Firstly, opening a speed controller 11 under a preassembled column A, allowing plasma water to enter a gravity column, cleaning a nickel column once, and then closing the speed controller 11 under the preassembled column A; secondly, opening the speed controller 11 under the preassembled column B, allowing the sample to enter the gravity column 5, and closing the speed controller 11 after the sample is completed; thirdly, opening a speed controller 11 under the preassembled column C to allow buffer solution to enter the gravity column 5; fourth, closing the speed controller 11 between the gravity column 5 and the beaker 3, and opening the speed controller 11 between the gravity column 5 and the test tube 4; fifthly, opening a speed controller 11 below the preassembled column D to allow eluent to enter the gravity column 5; after the experiment is completed, the liquid in the beaker 3 is the waste liquid, and the liquid in the test tube is the target protein.

Example 3;

unlike the above embodiments, the device is not limited to the use method of the above embodiments, and the reagents in the

pre-loading columns

6, 7, 8, 9 and the gravity column 5 can be flexibly replaced according to the types of the target protein to be extracted, such as the sample, the nickel column and other test materials, so as to meet the requirements of different experimental scenes.

Further, this a support 1 that is used for protein gravity purification device through using the design, a large amount of preparation work before having avoided the experiment, through using the speed controller 11, the speed that control experiment that can be better goes on, through adopting a plurality of pre-installation post A6, pre-installation post B7, pre-installation post C8, and pre-installation post D9, the rigor of experiment has been improved, let the experimental result more accurate, support 1 through adopting organic glass material preparation, let the experimentation more do benefit to the observation, through adopting the partial shipment structure, let the change of each part simpler, maintenance cost has been reduced, the environmental protection material has been adopted in the whole, but reuse after retrieving.

Working principle: the utility model relates to a gravity purifying device for protein, which uses the principle that the target protein or antibody is combined with the corresponding protein or antigen, and the combination is reversible under certain conditions. After the corresponding protein or antigen is solidified, the target protein or antibody existing in the liquid phase can be selectively combined on the solid phase carrier, thereby being separated from other proteins in the liquid phase, and achieving the purpose of separation and purification. This device is based on this principle design promptly, and the support of integration, can significantly reduce the preparation time before the experiment, a plurality of pre-installation posts can improve the rigor of experiment, and the accuse fast ware of being equipped can make things convenient for the experimenter to the control of experimental velocity, transparent material of whole body, more can conveniently observe experimental process.

While the fundamental and principal features of the utility model and advantages of the utility model have been shown and described, it will be apparent to those skilled in the art that the utility model is not limited to the details of the foregoing exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof; the present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims

1. A device for the gravitational purification of proteins, comprising a support (1), characterized in that: the support (1) is divided into three layers, a collecting frame (2) is arranged on a first layer, a beaker (3) and a test tube (4) are arranged on the collecting frame (2), a gravity column (5) is arranged on a second layer of the support (1), and four preassembling columns, namely a preassembling column A (6), a preassembling column B (7), a preassembling column C (8) and a preassembling column D (9), are arranged on a third layer of the support (1).

2. A gravity purification device for proteins according to claim 1, wherein: the bracket (1) is integrated and made of plexiglas.

3. A gravity purification device for proteins according to claim 1, wherein: the gravity column (5) is internally provided with a filter membrane, and scales are marked on the gravity column (5).

4. A gravity purification device for proteins according to claim 1, wherein: the preassembled column A (6), the preassembled column B (7), the preassembled column C (8) and the preassembled column D (9) are marked with scales.

5. A gravity purification device for proteins according to claim 1, wherein: the gravity column (5) is connected with the beaker (3) and the test tube (4) below through the hose (10), and the preassembled column is connected with the gravity column (5) below through the hose (10).

6. A gravity purification device for proteins according to claim 5, wherein: the hoses (10) are provided with speed controllers (11).

7. A gravity purification device for proteins according to claim 1, wherein: the size of the collecting frame (2) is just matched with the size of the first layer of the bracket (1).

8. A gravity purification device for proteins according to claim 1, wherein: the gravity column (5) is made of organic plastic, and the preassembled column A (6), the preassembled column B (7), the preassembled column C (8) and the preassembled column D (9) are made of organic plastic.

9. A gravity purification device for proteins according to claim 1, wherein: a locating hole is formed in the center of the second layer of the support (1), and four locating holes are formed in the third layer of the support (1).